PROCESS FOR PRODUCTION OF N-ACETYL-D-NEURAMINIC ACID

Title:

PROCESS FOR PRODUCTION OF N-ACETYL-D-NEURAMINIC ACID

Document Type and Number:

WIPO Patent Application WO/2011/086834

Kind Code:

A1

Abstract:

Disclosed are: NANA aldolase which is stable under alkaline conditions having high pH values; and a process for producing NANA in a large amount with high efficiency using the NANA aldolase. Specifically disclosed are: a protein which exhibits pH stability at pH 8.0 to 11.0 and has an N-acetyl-D-neuraminic acid aldolase activity; a polynucleotide encoding the protein; an expression vector carrying the polynucleotide; a transformant having the expression vector introduced thereinto; a bacterium capable of producing the protein; a process for producing N-acetyl-D-neuraminic acid aldolase using the protein; and others.

More Like This:

JP4860869	A method of amplifying and detecting multiple polynucleotides on a solid phase support
WO/1998/031806	Fc RECEPTORS AND POLYPEPTIDES
JP6257634	The new UDP-sugar transfer enzyme and its use of the Korea carrot origin

Inventors:

YOKOZEKI KENZO (JP)
HAGISHITA TAIRO (JP)
SHIMIZU SAKAYU (JP)

Application Number:

PCT/JP2010/073379

Publication Date:

July 21, 2011

Filing Date:

December 24, 2010

Export Citation:

Click for automatic bibliography generation Help

Assignee:

AJINOMOTO KK (JP)
YOKOZEKI KENZO (JP)
HAGISHITA TAIRO (JP)
SHIMIZU SAKAYU (JP)

International Classes:

C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/88; C12P19/26

Domestic Patent References:

WO1994029476A1

1994-12-22

Foreign References:

JPS61219384A	1986-09-29
JPH05211884A	1993-08-24

Other References:

TRAVING C. ET AL.: "Cloning, sequencing and expression of the acylneuraminate lyase gene from Clostridium perfringens A99", GLYCOCONJ. J., vol. 14, no. 7, 1997, pages 821 - 830
BLAYER S. ET AL.: "Alkaline biocatalysis for the direct synthesis of N-acetyl-D-neuraminic acid (Neu5Ac) from N-acetyl-D-glucosamine (GlcNAc)", BIOTECHNOL. BIOENG., vol. 66, no. 2, 1999, pages 131 - 136

Attorney, Agent or Firm:

SAKAI, Hiroaki et al. (JP)
Hiroaki Sakai (JP)

Download PDF:

View/Download PDF PDF Help

Claims:

Previous Patent: POWER-GENERATING UNIT

Next Patent: TWO-SHAFT CLAMP DEVICE