Title

Process for the synthesis of aryloxypropylamine and heteroaryloxypropylamine

Description

The present invention is related to a process for the synthesis of aryloxypropylamine and heteroaryloxypropylamine of formula I:

(I) where:

A is aryl or heteroaryl, where aryl is preferably a phenyl, optionally substituted, selected from benzyl and tolyl and the heteroaryl is preferably thiophenyl; Y is an aryl, preferably phenyl, a substituted phenyl or a naphthyl, where the substituted phenyl is preferably selected from tolyl, trihalomethyltolyl and alkoxytolyl; starting from a suitable amino alcohol of formula II:

(H) in which the hydroxyl is substituted with a halogen to give compounds of formula III, which are obtained as hydro -halogenated salts:

(II) (III)

The compounds of formula III are reacted with a protective agent of amino groups to give compounds of formula IV

(III) (IV)

where G indicates a protector group of the amino group, of formula R-C(=O)-, where R is an alkyl, optionally substituted, residue, aryl or alkoxy residue, or of formula R'-S(=O) ₂ - where R' indicates a alkyl residue optionally substituted or aryl residue.

The compounds of formula IV are reacted with the anions of the aryl alcohols of interest, preferably phenols, substituted phenols or naphthols to give the compounds of formula V

(IV) (V)

The compounds of formula V are then appropriately deprotected. PRIOR ART

Aryloxypropylamines are an important class of pharmaceutical products used in the care of attention-deficit disorder and hyperactivity disturbances. Some of the most important products belonging to this class are the following:

Fluoxetine - formula VI

Atomoxetine -formula VII

(VIII)

Nisoxetine - formula VIII

Duloxetine is a chemically very similar product having the following formula:

It is used as drug for the care of depression and incontinence. The known synthesis paths for obtaining this product type starting from the corresponding amino alcohol can be divided into two groups: A) Aromatic nucleophilic substitution processes like those described in WO2000290239.

This kind of process provides for the substitution of a halogen atom on the ring by the OH of the amino alcohol of formula II, suitably activated as an alcoholate. This process type is applicable with good results only when electron-attractor groups are present on the aromatic ring which bears the halogen to be substituted (in the diagram below, when A and X have the meanings defined above and Y' is an electron-attractor group).

(II) (X) (XI)

B) Nucleophilic substitution reactions of the hydroxyl of the amino alcohol. (see for example US4018895 and US4314081).

This kind of process normally provides for the following reaction sequence: a) protection of the amino group;

b) activation of the hydroxyl by means of its transformation into a good leavingt group (preferably a halogen); c) nucleophilic substitution with the appropriate aryloxy, optionally substituted; d) deprotection of the amino group

(H) (XII) (IV)

(XIII) (XI)

The processes of this type are longer but have a much wider range of application.

DESCRIPTION OF THE INVENTION

During the study of the synthesis of aryloxy and heteroaryloxy propylamine we surprisingly found that if in the processes of B type the order of the amino group protection and hydroxyl activation stages is reversed, greater overall yields are obtained. Moreover, taking into consideration that the activation of the hydroxyl is generally carried out in an acidic environment, reversing the two reaction stages permits using protective groups of the amino group which are not compatible with acidic environments.

The reaction sequence of the present invention is therefore the following: a) activation of the hydroxyl of an amino alcohol of formula II by means of its substitution with a halogen atom, preferably by means of reaction with a suitable halogenating agent ( for example of the type SOX 2, PX3, POX3, etc., where X

indicates a halogen), to obtain the salifϊed amino halogen-derivative of formula III.

A _H ^A N HX

(H) (III)

This reaction is carried out in a halogenated solvent, preferably methylene chloride, at a temperature in the range of 0 ⁰ C - 40 ⁰ C, preferably in the range of 20 ⁰ C - 25 ⁰ C. The quantity of halogenating agent, preferably thionyl chloride, is in the range of 0.8 - 3 equivalents with respect to the starting amino alcohol, preferably in the range of 1 - 2 equivalents. The reaction time is in the range of 1 - 4 hours, preferably in the range of 1 - 2 hours. b) protection of the amino group of the amino halogen-derivative of formula III, to obtain the protected amino halogen-derivative of formula IV.

(HI) (IV) where G is an amino protecting group.

The methods for protecting amines are well known in the art and are described for example in Greene et al., Protective Groups in Organic Synthesis, chapter 7,

Third Edition, 1999, Wiley-Interscience, pages 494-653, incorporated here for reference.

Preferably, G indicates a protector group of the amino group, preferably of formula R-C(=O)- where R is an alkyl residue, ptionally substituted, aryl or alkoxy residue, or of formula R'-S(=0)2- where R' indicates an alkyl, optionally substituted, alkyl residue or aryl residue.

The protection reaction is carried out in a halogenated solvent, preferably methylene chloride, and water, (in ratios in the range of 0.5 - 1.5 to 1, preferably 0.75 to 1) at a temperature in the range of 0 ⁰ C - 30 ⁰ C, preferably in the range of 5 ⁰ C - 10 ⁰ C, and in the presence of a base. The quantity of protective agent, which can be an activated derivative of an acyl, preferably an acetyl or a benzoyl, of a sulfonyl, preferably a tosyl, or of an oxyformyl, preferably a benzyloxyformyl or an ethoxyformyl, is in the range of 0.8 - 3 equivalents with respect to the starting amino halogen-derivative, preferably in the range of 1 - 2 equivalents. The quantity of base, preferably NaOH, is in the range of 1 - 4 equivalents with respect to the starting amino halogen-derivative, preferably in the range of 2 - 3 equivalents. The reaction time is in the range of 1 - 4 hours, preferably 1 - 2 hours, c) substitution of the halogen of the protected amino halogen-derivative of formula IV with the appropriate aryloxy, so to obtain the protected aryloxypropylamine (or heteroaryloxypropylamine) of formula XIII.

(IV) (XIII)

This reaction is carried out in an ether solvent, preferably polar, still more preferably tetrahydrofuran, at a temperature in the range of 25 ⁰ C - 65 ⁰ C, preferably 50 ⁰ C - 65 ⁰ C. The quantity of aryloxy, preferably obtained from o- cresol by means of a base, is in the range of 1 - 5 equivalents with respect to the starting protected amino halogen-derivative, preferably in the range of 2 - 4 equivalents. The quantity of base, preferably KOH, is in the range of 1 - 5 equivalents with respect to the starting protected amino halogen-derivative, preferably in the range of 2 - 4 equivalents. The reaction time is in the range of 5 - 20 hours, preferably in the range of 10 - 12 hours.

d) Deprotection of the formula compounds (XIII) to obtain the aryloxypropanolamine or heteroaryloxypropylamine of the invention, of formula (XI).

The deprotection reaction type to be used varies in relation to the protective group and is well known in the art (see Greene et al. above). In the case of amides or carbamates, hydrolysis reactions in basic environment are used.

If the carbamate is a benzylcarbamate, catalytic hydrogenolysis reactions are preferably utilised for the deprotection. Such hydrogenolysis reactions can be conducted with hydrogen, formic acid, ammonium formate, tertiary amine salts with formic acid or mixtures of formic acid and its salts.

The invention will be further illustrated by the following examples, which should not be considered as limiting the object of the invention.

Stage a) - Activation of the hydroxyl

Preparation of a compound of formula III

EXAMPLE 1

(XIV) (XV)

N-methyl-3-pheny 1-3 -hydroxy-propylamine (10Og; O.βOmoles) is loaded while being stirred at room temperature into methylene chloride (400ml), obtaining a solution. A solution Of SOCl ₂ (52.7ml; 0.73moles) is dripped in about 30 minutes

into methylene chloride (100ml), and the resulting liquid is stirred at room temperature for l-2h. The vacuum solvent is evaporated at 40 ⁰ C until a solid residue is attained. Acetone (400ml) is added; the suspension is left to stir for 30 minutes and the solvent is removed by vacuum evaporation. Acetone (500ml) is once again added; the suspension is reflux heated (56°C) for Ih, then it is cooled to room temperature and left stirring for Ih. The solid is filtered, washing the panel with acetone (100ml) and it is dried in a 50 ⁰ C oven so to obtain the compound of formula XV (117.6g; 88% yield).

Stage b) - Protection of the amino group

Preparation of a compound of formula IV

The protection of the amino group of the compounds of formula III can be carried out in different modes known in the art. Several of these are illustrated in examples 2-7.

Diagram of examples 2-3

(XV) (XVI)

G=

O ^' O (XVIII)

EXAMPLE 2

Compound of formula XVII (G = benzyloxyformyl)

NaOH (63.8g; 1.59moles) is loaded at 5/10 ⁰ C into H ₂ O (800ml). Ethyl acetate is added (585ml). The compound of formula XV is loaded (117g; 0.53moles) and finally, in about Ih and still at 5/10 ⁰ C, the benzyl chloroformate (83.5ml; 0.58moles) is loaded. The resulting mixture is stirred at 5/10 ⁰ C for 2-3h. The 2

phases are separated; the organic phase is vacuum evaporated to form an oily residue (compound of formula XVII; 191g; 94% yield), which is used as is for the subsequent step (see example 8).

EXAMPLE 3

Compound of formula XVIII (G = ethoxyformyl)

In an analogous manner, the compound of formula XVIII (which is used for such for the subsequent step, see example 8) is prepared by using ethyl chloroformate.

Diagram of examples 4-6

(XV) (XVI)

EXAMPLE 4

Compounds of formula XIX (G = acetyl)

The compound XV (55g; 0.25moles) is loaded at room temperature into methylene chloride (550ml) and triethylamine (83ml; O.βmoles). Acetyl chloride (21,3ml; 0.3moles) is added, still at room temperature and in about 30 minutes. The resulting mixture is stirred at room temperature for Ih. H ₂ O (200ml) is added and the 2 phases are separated; the organic phase is vacuum evaporated to form an

oily residue ( compound XIX) ; 55g; 97% yield), which is used as is for the subsequent step (see example 8).

EXAMPLE 5

Compound of formula XX (G = benzoyl)

In an analogous manner, the compound of formula XX is prepared (which is used as is for the subsequent step, see example 8) by utilising benzoyl chloride.

EXAMPLE 6

Compound of formula XXI (G = tosyl)

In an analogous manner, the compound of formula XXI (which is used as is for the subsequent step, see example 8) is prepared by using tosyl chloride.

Diagram of example 7

(XV) (XVI)

G= (At- (XXII)

EXAMPLE 7

Compound of formula XXII (G = t-butoxyformyl)

H ₂ O (135ml) and 30% NaOH (30ml; 0.3moles) are mixed at room temperature.

The compound of formula XV (3Og; 0.14moles) and t-BuOH (135ml) are added.

Finally, BoC ₂ O (32.7g; 0.15moles) is loaded to portions in about 30 minutes. The resulting mixture is stirred at room temperature for Ih. The 2 phases are separated; the organic phase is vacuum evaporated to form an oily residue

(compound of formula XXII; 35g; 90% yield), which is utilised as such for the subsequent step (see example 8).

Stage c) - Substitution

Preparation of a compound of formula V

EXAMPLE 8

(XVI) (XXIII)

KOH (135.9g; 2.18moles) is loaded at room temperature into THF (420ml), and this is heated to 50/60 ⁰ C. 0-cresol (23Og; 2.13moles) is added into THF (230ml). The resulting mixture is stirred at 50/60 ⁰ C for Ih, then a solution of compound XVI (168g; 0.53mol) is added into THF (170ml). The reaction mixture is reflux heated (66°C), and stirred for 12h. It is cooled to room temperature; the THF is evaporated, toluene (400ml) and 10% NaOH (100ml) are added; the 2 phases are separated; the organic phase is washed with 10% NaOH (2* 100ml). The combined organic phases are vacuum evaporated to form a small residue. Methanol (250ml) is added at 50/60 ⁰ C, and the resultant is cooled at 0/5 ⁰ C for 2- 3h. Filtering is then carried out, washing the panel with methanol (50ml), and the solid is vacuum dried at 50 ⁰ C to obtain the compound XXIII (154g; 74.4% yield). Stage d) — Deprotection Preparation of a compound of formula VII

EXAMPLE 9

(XXIII) (VII)

The compound of formula VII is obtained from the compound of formula XXIII, by means of methods known in literature.

EXAMPLE 10

Compound of formula XXIV

(VII) (XXIV)

The compound of formula XXIV is obtained from the compound VII, by means of methods known in literature.