Title:
PROCESSES FOR ADENOVIRUS PURIFICATION USING CONTINUOUS FLOW CENTRIFUGATION
Document Type and Number:
WIPO Patent Application WO2005086658
Kind Code:
A3
Abstract:
The present invention relates to methods for the scalable preparation for adenoviral preparations comprising the steps of: culturing host cells comprising adenovirus; obtaining supernatants from the host cells; applying said supernatants to a centrifugal apparatus comprising a 50% w/v solution of non-ionic gradient; applying centrifugal force to said supernatants such that the flow is continuous and directed from bottom-to-top; separating the adenoviral particles according to their density; and obtaining high-yield fractions comprising active adenoviral particles.
Inventors:
FORRESTER KATHY (US)
Application Number:
PCT/US2005/005810
Publication Date:
October 05, 2006
Filing Date:
February 25, 2005
Export Citation:
Assignee:
ALFA WASSERMANN INC (US)
International Classes:
B01D17/038; B01D17/02; C12N7/00; C12N15/86
Foreign References:
| US20050272029A1 | 2005-12-08 | |||
| EP1284287A1 | 2003-02-19 |
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