Title:
PROCESSES USING VLPS WITH CAPSIDS RESISTANT TO HYDROLASES
Document Type and Number:
WIPO Patent Application WO/2013/096866
Kind Code:
A3
Abstract:
Novel processes and compositions are described which use viral capsid proteins resistant to hydrolases to prepare virus-like particles to enclose and subsequently isolate and purify target cargo molecules of interest including nucleic acids such as siRNA's and shRNA's, and small peptides.
Inventors:
ARHANCET JUAN PEDRO HUMBERTO (US)
ARHANCET JUAN P (US)
DELANEY KIMBERLY (US)
HALL KATHLEEN B (US)
SUMMERS NEENA (US)
ARHANCET JUAN P (US)
DELANEY KIMBERLY (US)
HALL KATHLEEN B (US)
SUMMERS NEENA (US)
Application Number:
PCT/US2012/071419
Publication Date:
October 03, 2013
Filing Date:
December 21, 2012
Export Citation:
Assignee:
APSE LLC (US)
ARHANCET JUAN PEDRO HUMBERTO (US)
ARHANCET JUAN P (US)
DELANEY KIMBERLY (US)
HALL KATHLEEN B (US)
SUMMERS NEENA (US)
ARHANCET JUAN PEDRO HUMBERTO (US)
ARHANCET JUAN P (US)
DELANEY KIMBERLY (US)
HALL KATHLEEN B (US)
SUMMERS NEENA (US)
International Classes:
C12N7/00; C07H21/00
Domestic Patent References:
| WO2009095791A1 | 2009-08-06 |
Foreign References:
| US20110250675A1 | 2011-10-13 | |||
| US20060177819A1 | 2006-08-10 | |||
| US20030099668A1 | 2003-05-29 |
Other References:
RHEE ET AL.: "Colorful Virus-Like Particles: Fluorescent Protein Packaging by the Qbeta Capsid.", BIOMACROMOLECULES, vol. 12, no. 11, 14 November 2011 (2011-11-14), pages 3977 - 3981, XP055155114
BEEN ET AL.: "Optimal self-cleavage activity of the hepatitis delta virus RNA is dependent on a homopurine base pair the ribozyme core.", RNA, vol. 1, no. 10, December 1995 (1995-12-01), pages 1061 - 1070, XP055155116
ZLOTNICK ET AL.: "Localization of the C terminus of the assembly domain of hepatitis B virus capsid protein: Implications for morphogenesis and organization of encapsidated RNA.", PROC NATL ACAD SCI USA, vol. 94, no. 18, September 1997 (1997-09-01), pages 9556 - 9561, XP002982400
LI ET AL.: "Essential Elements of the Capsid Protein for Self-Assembly into Empty Virus-Like Particles of Hepatitis E Virus.", J VIROLOGY, vol. 79, no. 20, October 2005 (2005-10-01), pages 12999 - 13006, XP002718141
MORGENSTERN ET AL.: "Multiple sequence alignment with user-defined anchor points.", ALGORITHMS FOR MOLECULAR BIOLOGY, vol. 1, no. 1, 19 April 2006 (2006-04-19), pages 6, XP021020303
PATKAR ET AL.: "Functional Requirements of the Yellow Fever Virus Capsid Protein.", J VIROLOGY, vol. 81, no. 12, June 2007 (2007-06-01), pages 6471 - 6481, XP002532339
BEAMES ET AL.: "Insertions within the Hepatitis B Virus Capsid Protein Influence Capsid Formation and RNA Encapsidation.", J VIROLOGY, vol. 69, no. 11, November 1995 (1995-11-01), pages 6833 - 6838, XP055155119
BEEN ET AL.: "Optimal self-cleavage activity of the hepatitis delta virus RNA is dependent on a homopurine base pair the ribozyme core.", RNA, vol. 1, no. 10, December 1995 (1995-12-01), pages 1061 - 1070, XP055155116
ZLOTNICK ET AL.: "Localization of the C terminus of the assembly domain of hepatitis B virus capsid protein: Implications for morphogenesis and organization of encapsidated RNA.", PROC NATL ACAD SCI USA, vol. 94, no. 18, September 1997 (1997-09-01), pages 9556 - 9561, XP002982400
LI ET AL.: "Essential Elements of the Capsid Protein for Self-Assembly into Empty Virus-Like Particles of Hepatitis E Virus.", J VIROLOGY, vol. 79, no. 20, October 2005 (2005-10-01), pages 12999 - 13006, XP002718141
MORGENSTERN ET AL.: "Multiple sequence alignment with user-defined anchor points.", ALGORITHMS FOR MOLECULAR BIOLOGY, vol. 1, no. 1, 19 April 2006 (2006-04-19), pages 6, XP021020303
PATKAR ET AL.: "Functional Requirements of the Yellow Fever Virus Capsid Protein.", J VIROLOGY, vol. 81, no. 12, June 2007 (2007-06-01), pages 6471 - 6481, XP002532339
BEAMES ET AL.: "Insertions within the Hepatitis B Virus Capsid Protein Influence Capsid Formation and RNA Encapsidation.", J VIROLOGY, vol. 69, no. 11, November 1995 (1995-11-01), pages 6833 - 6838, XP055155119
Attorney, Agent or Firm:
NEALEY, Tara A. et al. (100 South Fourth Street Suite 100, St. Louis MO, US)
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