Title:
PRODUCTION OF 1,4 BUTANEDIOL IN A MICROORGANISM
Document Type and Number:
WIPO Patent Application WO/2010/085731
Kind Code:
A3
Abstract:
A biological method for the conversion of L-glutamate to 1,4-butanediol that involves a decarboxylation step and avoids production of 4-hydroxybutyrate as an intermediate is described. The method includes: (a) conversion of L-glutamate to L-glutamate 5- phosphate; (b) conversion of L-glutamate 5 -phosphate to L-glutamate 5-semialdehyde; (c) conversion of L-glutamate 5-semialdehyde to 5-hydroxy-L-norvaline;(d) conversion of 5-hydroxy-L-norvaline to 5-hydroxy-2-oxopentanoate; (e) conversion of 5-hydroxy-2- oxopentanoate to 4-hydroxybutanal; and (f) the conversion of 4-hydroxybutanal to 1,4- butanediol.
Inventors:
MADDEN KEVIN T (US)
YOUNG DAVID M (US)
BOWER STANLEY (US)
LIU CHI-LI (US)
YOUNG DAVID M (US)
BOWER STANLEY (US)
LIU CHI-LI (US)
Application Number:
PCT/US2010/021952
Publication Date:
November 25, 2010
Filing Date:
January 25, 2010
Export Citation:
Assignee:
MICROBIA INC (US)
TATE & LYLE INGREDIENTS (US)
MADDEN KEVIN T (US)
YOUNG DAVID M (US)
BOWER STANLEY (US)
LIU CHI-LI (US)
TATE & LYLE INGREDIENTS (US)
MADDEN KEVIN T (US)
YOUNG DAVID M (US)
BOWER STANLEY (US)
LIU CHI-LI (US)
International Classes:
C12N1/21; C12N15/74; C12P7/18; C12P13/00
Domestic Patent References:
| WO2007030830A2 | 2007-03-15 | |||
| WO2009011974A1 | 2009-01-22 |
Foreign References:
| KR20070096348A | 2007-10-02 | |||
| JPS62285779A | 1987-12-11 |
Other References:
YU ZHENG ET AL.: "One-step production of 2,3-butanediol from starch by secretory over-expression of amylase in Klebsiella pneumoniae.", J CHEM TECHNOL BIOTECHNOL., vol. 83, no. 10, 2008, pages 1409 - 1412
HIKMET GECKI ET AL.: "Enhanced production of acetoin and butanediol in recombinant Enterobacter aerogenes carrying Vitreoscilla hemoglobin gene.", BIOPROCESS BIOSYST ENG., vol. 26, no. 5, 2004, pages 325 - 330
HIKMET GECKI ET AL.: "Enhanced production of acetoin and butanediol in recombinant Enterobacter aerogenes carrying Vitreoscilla hemoglobin gene.", BIOPROCESS BIOSYST ENG., vol. 26, no. 5, 2004, pages 325 - 330
Attorney, Agent or Firm:
MEIKLEJOHN, Anita, L. (P.O. Box 1022Minneapolis, MN, US)
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