Title:
PRODUCTION OF 3-FUCOSYLLACTOSE AND LACTOSE CONVERTING α-1,3-FUCOSYLTRANSFERASE ENZYMES
Document Type and Number:
WIPO Patent Application WO/2020/127417
Kind Code:
A3
Abstract:
The present invention relates to methods for producing 3-fucosyllactose (3-FL) as well as novel fucosyltransferases, more specifically novel lactose binding alpha-1,3-fucosyltransferase polypeptides, and their applications. Furthermore, the present invention provides methods for producing3-fucosyllactose (3-FL) using the novel lactose binding alpha-1,3-fucosyltransferases.
Inventors:
BEAUPREZ JOERI (BE)
LANNOO NAUSICAÄ (BE)
VANDEWALLE KRISTOF (BE)
VERCAUTEREN ANNELIES (BE)
LANNOO NAUSICAÄ (BE)
VANDEWALLE KRISTOF (BE)
VERCAUTEREN ANNELIES (BE)
Application Number:
PCT/EP2019/085841
Publication Date:
July 30, 2020
Filing Date:
December 18, 2019
Export Citation:
Assignee:
INBIOSE NV (BE)
International Classes:
C07H3/06; C07H5/06; C12N9/10; C12P19/18
Domestic Patent References:
| WO2016040531A1 | 2016-03-17 |
Other References:
K L WHITESON: "Uncharacterized protein from Basilea psittacipulmonis DSM 2470", 5 December 2018 (2018-12-05), XP055667225, Retrieved from the Internet [retrieved on 20200211]
MARTIN S L ET AL: "Lewis X biosynthesis in Helicobacter pylori", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 272, no. 34, 22 August 1997 (1997-08-22), pages 21349 - 21356, XP002085414, ISSN: 0021-9258, DOI: 10.1074/JBC.272.34.21349
PETSCHACHER BARBARA ET AL: "Biotechnological production of fucosylated human milk oligosaccharides: Prokaryotic fucosyltransferases and their use in biocatalytic cascades or whole cell conversion systems", JOURNAL OF BIOTECHNOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 235, 1 April 2016 (2016-04-01), pages 61 - 83, XP029733274, ISSN: 0168-1656, DOI: 10.1016/J.JBIOTEC.2016.03.052
MARTIN S L ET AL: "Lewis X biosynthesis in Helicobacter pylori", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 272, no. 34, 22 August 1997 (1997-08-22), pages 21349 - 21356, XP002085414, ISSN: 0021-9258, DOI: 10.1074/JBC.272.34.21349
PETSCHACHER BARBARA ET AL: "Biotechnological production of fucosylated human milk oligosaccharides: Prokaryotic fucosyltransferases and their use in biocatalytic cascades or whole cell conversion systems", JOURNAL OF BIOTECHNOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 235, 1 April 2016 (2016-04-01), pages 61 - 83, XP029733274, ISSN: 0168-1656, DOI: 10.1016/J.JBIOTEC.2016.03.052
Attorney, Agent or Firm:
SAELENS, Claire (BE)
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