Title:
PRODUCTION OF C-TERMINAL AMIDATED PEPTIDES FROM REPRODUCTION OF C-TERMINAL AMIDATED PEPTIDES FROM RECOMBINANT PROTEIN CONSTRUCTS COMBINANT PROTEIN CONSTRUCTS
Document Type and Number:
WIPO Patent Application WO1996017941
Kind Code:
A3
Abstract:
A method for the production of C-terminal amidated recombinant peptides is provided. The method employs a recombinant protein construct having multiple copies of a target peptide linked by intraconnecting peptides. The intraconnecting peptides permit the multicopy construct to be selectively reacted to produce product peptides having a C-terminal alpha -carboxamide. A recombinant gene containing a DNA sequence coding for the recombinant protein construct and an expression cassette, an expression vector and a transformed cell including the recombinant gene are also provided.
Inventors:
STOUT JAY S
PATRIDGE BRUCE E
HERIKSEN DENNIS B
HOLMQUIST BARTON
WAGNER FRED W
PATRIDGE BRUCE E
HERIKSEN DENNIS B
HOLMQUIST BARTON
WAGNER FRED W
Application Number:
PCT/US1995/015799
Publication Date:
August 22, 1996
Filing Date:
December 07, 1995
Export Citation:
Assignee:
BIONEBRASKA INC (US)
International Classes:
C07K1/113; C07K14/60; C07K14/605; C07K14/635; C12N9/88; C12N15/62; C12P21/02; C12P21/06; (IPC1-7): C12N15/62; C07K1/113; C07K7/22; C07K14/60; C07K14/605; C07K14/635; C12N1/21; C12N15/16; C12N15/60; C12P21/06
Domestic Patent References:
| WO1992001707A1 | 1992-02-06 | |||
| WO1990008194A1 | 1990-07-26 |
Foreign References:
| EP0499990A2 | 1992-08-26 | |||
| EP0401657A1 | 1990-12-12 |
Other References:
DATABASE CAPLUS [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; AASMUL-OLSEN, STIG ET AL: "Carboxypeptidase mediated C - terminal amidation of polypeptide acids", XP002005078, accession no. STN Database accession no. 1992:512045
U. MORTENSEN ET AL: "Site-directed mutagenesis on (Serine) Carboxypeptidase Y", BIOCHEMISTRY, vol. 33, 1994, pages 508 - 517, XP002005076
ENGELS J W ET AL: "ENZYMATIC AMIDATION OF RECOMBINANT (LEU27) GROWTH HORMONE RELEASING HORMONE-GLY45", PROTEIN ENGINEERING, EYNSHAM, OXFORD, GB, vol. 1, no. 3, June 1987 (1987-06-01), pages 195 - 199, XP000000195
D. HENRIKSEN ET AL: "Peptide amidation by chemical protein engineering", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 114, 1992, DC US, pages 1876 - 1877, XP002005077
U. MORTENSEN ET AL: "Site-directed mutagenesis on (Serine) Carboxypeptidase Y", BIOCHEMISTRY, vol. 33, 1994, pages 508 - 517, XP002005076
ENGELS J W ET AL: "ENZYMATIC AMIDATION OF RECOMBINANT (LEU27) GROWTH HORMONE RELEASING HORMONE-GLY45", PROTEIN ENGINEERING, EYNSHAM, OXFORD, GB, vol. 1, no. 3, June 1987 (1987-06-01), pages 195 - 199, XP000000195
D. HENRIKSEN ET AL: "Peptide amidation by chemical protein engineering", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 114, 1992, DC US, pages 1876 - 1877, XP002005077
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