Title:
PRODUCTION OF CARRIER-PEPTIDE CONJUGATES USING CHEMICALLY REACTIVE UNNATURAL AMINO ACIDS
Document Type and Number:
WIPO Patent Application WO/2010/068278
Kind Code:
A3
Abstract:
Provided are methods of making carrier polypeptide that include incorporating a first unnatural amino acid into a carrier polypeptide variant, incorporating a second unnatural amino acid into a target polypeptide variant, and reacting the first and second unnatural amino acids to produce the conjugate. Conjugates produced using the provided methods are also provided. In addition, orthogonal translation systems in methylotrophic yeast and methods of using these systems to produce carrier and target polypeptide variants comprising unnatural amino acids are provided.
Inventors:
YOUNG TRAVIS (US)
SCHULTZ PETER G (US)
SCHULTZ PETER G (US)
Application Number:
PCT/US2009/006500
Publication Date:
December 29, 2010
Filing Date:
December 09, 2009
Export Citation:
Assignee:
SCRIPPS RESEARCH INST (US)
YOUNG TRAVIS (US)
SCHULTZ PETER G (US)
YOUNG TRAVIS (US)
SCHULTZ PETER G (US)
International Classes:
C07K19/00; C07K14/39; C07K16/16; C12N5/10
Domestic Patent References:
| WO2007136778A2 | 2007-11-29 |
Foreign References:
| US20070160598A1 | 2007-07-12 | |||
| US20070202552A1 | 2007-08-30 |
Other References:
I. NEUNDROF ET AL.: "Detailed Analysis Concerning the Biodistribution and Metabolism of Human Calcitonin-Derived Cell-Penetrating Peptides", BIOCONJUGAT E CHEM., vol. 19, August 2008 (2008-08-01), pages 1596 - 1603, XP002575961
Attorney, Agent or Firm:
QUINE, Jonathan, Alan et al. (P.C.P.O.box 45, Alameda CA, US)
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