Title:
PRODUCTION METHOD FOR BACTERIAL CELLS
Document Type and Number:
WIPO Patent Application WO/2019/059396
Kind Code:
A1
Abstract:
The present invention provides a production method for bacterial cells that, in a multi-stage liquid cultivation process for bacteria, includes a step in which, in the stage prior to the final stage, the bacteria are cultivated using a liquid medium containing an antibiotic substance, and in the final stage, the bacteria are cultivated upon reducing the concentration of the antibiotic substance to below that of the prior stage. It is thereby possible to produce the bacterial cells with excellent yield while suppressing the appearance of mutant individuals forming aberrant colonies in the bacteria obtained after cultivation.
Inventors:
MORISHITA YASUYUKI (JP)
Application Number:
PCT/JP2018/035361
Publication Date:
March 28, 2019
Filing Date:
September 25, 2018
Export Citation:
Assignee:
IDEMITSU KOSAN CO (JP)
International Classes:
C12N1/20; C12N1/00
Domestic Patent References:
| WO2016163534A1 | 2016-10-13 | |||
| WO2015056666A1 | 2015-04-23 | |||
| WO2018066686A1 | 2018-04-12 |
Other References:
OCHI, KOZO ET AL.: "Effect of Antibiotics on Sporulation Caused by the Stringent Response in Bacillus subtilis", OURNAL OF GENERAL MICROBIOLOGY, vol. 129, 1983, pages 3709 - 3720, XP055498642
CARLISLE, G. E. ET AL.: "Enzyme Activities and Antibiotic Susceptibility of Colonial Variants of Bacillus subtilis and Bacillus licheniformis", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 55, no. 11, 1989, pages 3026 - 3028, XP055584210
BIOTECHNOLOGY PROGRESS, vol. 21, no. 4, 2005, pages 1026 - 1031
APPL. MICROBIOL. BIOTECHNOL., vol. 99, no. 4, 2015, pages 1897 - 1910
ZHIWU BINGLI XUEBAO, vol. 38, no. 2, 2008, pages 185 - 191
APPL. ENVIRON. MICROBIOL., vol. 73, no. 20, 2007, pages 6620 - 6628
APPL. ENVIRON. MICROBIOL., vol. 55, 1989, pages 3026 - 3028
See also references of EP 3690025A4
CARLISLE, G. E. ET AL.: "Enzyme Activities and Antibiotic Susceptibility of Colonial Variants of Bacillus subtilis and Bacillus licheniformis", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 55, no. 11, 1989, pages 3026 - 3028, XP055584210
BIOTECHNOLOGY PROGRESS, vol. 21, no. 4, 2005, pages 1026 - 1031
APPL. MICROBIOL. BIOTECHNOL., vol. 99, no. 4, 2015, pages 1897 - 1910
ZHIWU BINGLI XUEBAO, vol. 38, no. 2, 2008, pages 185 - 191
APPL. ENVIRON. MICROBIOL., vol. 73, no. 20, 2007, pages 6620 - 6628
APPL. ENVIRON. MICROBIOL., vol. 55, 1989, pages 3026 - 3028
See also references of EP 3690025A4
Attorney, Agent or Firm:
SANUKI, Shinichi et al. (JP)
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