Title:
PRODUCTION METHOD, BROCCOLI, AND SEED
Document Type and Number:
WIPO Patent Application WO/2024/063076
Kind Code:
A1
Abstract:
The present invention provides a crop having more suitable properties by using genome editing, and promotes understanding of genome editing. The present invention uses, as target broccoli, broccoli to be subjected to genetic manipulation, and involves performing genetic manipulation for knocking out, among the genes of the target broccoli, ACO3 that causes production of ethylene which deteriorates the broccoli in a predetermined post-harvesting period. Next, seeds are produced from the target broccoli after the genetic manipulation. The seeds are sown, and grown into broccoli to be shipped. As a result, broccoli to be shipped is produced.
Inventors:
KOIZUKA NOBUYA (JP)
Application Number:
PCT/JP2023/034032
Publication Date:
March 28, 2024
Filing Date:
September 20, 2023
Export Citation:
Assignee:
TAMAGAWA UNIV AND TAMAGAWA ACADEMY (JP)
International Classes:
C12N15/09; A01H1/06; A01H6/20
Foreign References:
| JP2020521512A | 2020-07-27 |
Other References:
YANG CHIH-YUAN, CHU FANG HUA, WANG YUH TAI, CHEN YU-TING, YANG SHANG FA, SHAW JEI-FU: "Novel Broccoli 1-Aminocyclopropane-1-carboxylate Oxidase Gene ( Bo-ACO3 ) Associated with the Late Stage of Postharvest Floret Senescence", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 51, no. 9, 1 April 2003 (2003-04-01), US , pages 2569 - 2575, XP093150230, ISSN: 0021-8561, DOI: 10.1021/jf034007m
GAPPER N E, ET AL.: "AGROBACTERIUM TUMEFACIENS-MEDIATED TRANSFORMATION TO ALTER ETHYLENE AND CYTOKININ BIOSYNTHESIS IN BROCCOLI", PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC), SPRINGER NETHERLANDS, DORDRECHT, vol. 70, no. 01, 1 July 2002 (2002-07-01), Dordrecht, pages 41 - 50, XP008033202, ISSN: 0167-6857, DOI: 10.1023/A:1016057208267
SUZUKI YASUO, ASODA TOSHIYA, MATSUMOTO YUKIE, TERAI HIROFUMI, KATO MASAYA: "Suppression of the expression of genes encoding ethylene biosynthetic enzymes in harvested broccoli with high temperature treatment", POSTHARVEST BIOLOGY AND TECHNOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 36, no. 3, 1 June 2005 (2005-06-01), AMSTERDAM, NL , pages 265 - 271, XP093150232, ISSN: 0925-5214, DOI: 10.1016/j.postharvbio.2005.02.005
GAPPER N E, ET AL.: "AGROBACTERIUM TUMEFACIENS-MEDIATED TRANSFORMATION TO ALTER ETHYLENE AND CYTOKININ BIOSYNTHESIS IN BROCCOLI", PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC), SPRINGER NETHERLANDS, DORDRECHT, vol. 70, no. 01, 1 July 2002 (2002-07-01), Dordrecht, pages 41 - 50, XP008033202, ISSN: 0167-6857, DOI: 10.1023/A:1016057208267
SUZUKI YASUO, ASODA TOSHIYA, MATSUMOTO YUKIE, TERAI HIROFUMI, KATO MASAYA: "Suppression of the expression of genes encoding ethylene biosynthetic enzymes in harvested broccoli with high temperature treatment", POSTHARVEST BIOLOGY AND TECHNOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 36, no. 3, 1 June 2005 (2005-06-01), AMSTERDAM, NL , pages 265 - 271, XP093150232, ISSN: 0925-5214, DOI: 10.1016/j.postharvbio.2005.02.005
Attorney, Agent or Firm:
IWAIKE Mitsuru et al. (JP)
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