Title:
PRODUCTION METHOD FOR CELL MASS INCLUDING NEURAL CELLS/TISSUE AND NON-NEURAL EPITHELIAL TISSUE, AND CELL MASS FROM SAME
Document Type and Number:
WIPO Patent Application WO/2019/103125
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a means of efficiently producing, from pluripotent stem cells, a cell mass that includes neural cells/tissue and non-neural epithelial tissue. Provided is a production method for a cell mass that includes 1) neural cells or neural tissue, and 2) non-neural epithelial tissue, said method comprising steps (1) and (2): (1) a first step in which pluripotent stem cells are suspension-cultured in the presence of a Wnt-signalling pathway inhibitor, and a cell aggregation is formed; and (2) a second step in which the aggregate obtained in the first step is suspension-cultured in the presence of a BMP-signalling pathway activator, and a cell mass that includes 1) neural cells/tissue, and 2) non-neural epithelial tissue, is obtained.
Inventors:
NAKANO TOKUSHIGE (JP)
Application Number:
PCT/JP2018/043280
Publication Date:
May 31, 2019
Filing Date:
November 22, 2018
Export Citation:
Assignee:
SUMITOMO CHEMICAL CO (JP)
International Classes:
C12N5/0793; A61K35/30; A61K35/545; A61L27/36; A61L27/38; A61P27/02; C12N5/071; C12N5/079; C12Q1/02
Domestic Patent References:
WO2017183732A1 | 2017-10-26 | |||
WO2015020091A1 | 2015-02-12 | |||
WO2016114285A1 | 2016-07-21 | |||
WO2017043604A1 | 2017-03-16 | |||
WO2016063986A1 | 2016-04-28 | |||
WO2013086236A2 | 2013-06-13 | |||
WO2016167372A1 | 2016-10-20 | |||
WO2015020091A1 | 2015-02-12 | |||
WO1996022362A1 | 1996-07-25 | |||
WO2002101057A1 | 2002-12-19 | |||
WO1998030679A1 | 1998-07-16 |
Foreign References:
JP2017111065A | 2017-06-22 | |||
US5843780A | 1998-12-01 | |||
US6200806B1 | 2001-03-13 | |||
US6280718B1 | 2001-08-28 | |||
JP2001508302A | 2001-06-26 | |||
JP2017226308A | 2017-12-28 |
Other References:
FOSTER ET AL., SCIENTIFIC REPORTS, vol. 7, 2017
FU ET AL., INVESTIGATIVE OPHTHALMOLOGY VISUAL SCIENCE, vol. 58.1, 2017, pages 517 - 527
HAYASHI ET AL., NATURE, vol. 531.7594, 2016, pages 376 - 380
CELL, vol. 70, 1992, pages 841 - 847
CELL, vol. 126, no. 4, 2006, pages 663 - 676
CELL, vol. 131, no. 5, 2007, pages 861 - 872
SCIENCE, vol. 318, no. 5858, 2007, pages 1917 - 1920
NAT. BIOTECHNOL., vol. 26, no. 1, 2008, pages 101 - 106
SCIENCE, vol. 341, 2013, pages 651 - 654
"Manipulating the Mouse Embryo, A Laboratory Manual", 1994, COLD SPRING HARBOR LABORATORY PRESS
"Gene Targeting, A Practical Approach", 1993, IRL PRESS AT OXFORD UNIVERSITY PRESS
"Biomanual Series 8, Gene Targeting, Making of Mutant Mouse using ES cell", 1995, YODOSHA CO., LTD.
"Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
J. CELL BIOL., vol. 105, 1987, pages 589 - 598
CELL STEM CELL, vol. 10, no. 6, 2012, pages 771 - 775
NATURE METHODS, vol. 8, 2011, pages 424 - 429
NAKAGAWA ET AL., SCIENTIFIC REPORTS, vol. 4, 2014, pages 3594
NAT BIOTECHNOL, vol. 28, 2010, pages 611 - 615
NAT COMMUN, vol. 3, 2012, pages 1236
NAT BIOTECHNOL, vol. 19, 2001, pages 971 - 974
EMBO J., vol. 3, 1984, pages 1463 - 1468
ONCOGENE, vol. 26, 2007, pages 5163 - 5168
AM J CANCER RES., vol. 5, no. 8, 2015, pages 2344 - 2360
DEV BIOL., vol. 361, no. 2, 15 January 2012 (2012-01-15), pages 392 - 402
BIOCHEM J., vol. 402, 15 March 2007 (2007-03-15), pages 515 - 523
See also references of EP 3715452A4
FU ET AL., INVESTIGATIVE OPHTHALMOLOGY VISUAL SCIENCE, vol. 58.1, 2017, pages 517 - 527
HAYASHI ET AL., NATURE, vol. 531.7594, 2016, pages 376 - 380
CELL, vol. 70, 1992, pages 841 - 847
CELL, vol. 126, no. 4, 2006, pages 663 - 676
CELL, vol. 131, no. 5, 2007, pages 861 - 872
SCIENCE, vol. 318, no. 5858, 2007, pages 1917 - 1920
NAT. BIOTECHNOL., vol. 26, no. 1, 2008, pages 101 - 106
SCIENCE, vol. 341, 2013, pages 651 - 654
"Manipulating the Mouse Embryo, A Laboratory Manual", 1994, COLD SPRING HARBOR LABORATORY PRESS
"Gene Targeting, A Practical Approach", 1993, IRL PRESS AT OXFORD UNIVERSITY PRESS
"Biomanual Series 8, Gene Targeting, Making of Mutant Mouse using ES cell", 1995, YODOSHA CO., LTD.
"Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
J. CELL BIOL., vol. 105, 1987, pages 589 - 598
CELL STEM CELL, vol. 10, no. 6, 2012, pages 771 - 775
NATURE METHODS, vol. 8, 2011, pages 424 - 429
NAKAGAWA ET AL., SCIENTIFIC REPORTS, vol. 4, 2014, pages 3594
NAT BIOTECHNOL, vol. 28, 2010, pages 611 - 615
NAT COMMUN, vol. 3, 2012, pages 1236
NAT BIOTECHNOL, vol. 19, 2001, pages 971 - 974
EMBO J., vol. 3, 1984, pages 1463 - 1468
ONCOGENE, vol. 26, 2007, pages 5163 - 5168
AM J CANCER RES., vol. 5, no. 8, 2015, pages 2344 - 2360
DEV BIOL., vol. 361, no. 2, 15 January 2012 (2012-01-15), pages 392 - 402
BIOCHEM J., vol. 402, 15 March 2007 (2007-03-15), pages 515 - 523
See also references of EP 3715452A4
Attorney, Agent or Firm:
TAKASHIMA, Hajime et al. (JP)
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