Title:
PRODUCTION METHOD FOR COPOLYMER POLYESTER USING RECOMBINANT STRAIN HAVING MODIFIED PROTEIN LOCUS FOR POLYESTER GRANULE BINDING
Document Type and Number:
WIPO Patent Application WO/2015/133468
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method by which the composition ratio of monomer units in a copolymer polyhydroxyalkanoic acid is controlled. Provided is a method by which the composition ratio of monomer units within a copolymer PHA is controlled, and in which, in a recombinant strain having copolymer PHA productivity imparted thereto by the insertion of one of more genes that code a polyhydroxyalkanoic acid (PHA) polymer enzyme having wide-substrate specificity into a host that is different to the living organism of the inserted gene, a gene that codes the main PHA granule binding protein in the recombinant strain is made into a gene that codes a PHA granule binding protein deriving from the same living organism as the gene that codes the PHA polymer enzyme.
Inventors:
FUKUI TOSHIAKI (JP)
ORITA IZUMI (JP)
KAWASHIMA YUI (JP)
ORITA IZUMI (JP)
KAWASHIMA YUI (JP)
Application Number:
PCT/JP2015/056201
Publication Date:
September 11, 2015
Filing Date:
March 03, 2015
Export Citation:
Assignee:
TOKYO INST TECH (JP)
KANEKA CORP (JP)
KANEKA CORP (JP)
International Classes:
C12N15/09; C12P7/62
Domestic Patent References:
| WO2009145164A1 | 2009-12-03 | |||
| WO2011105379A1 | 2011-09-01 | |||
| WO2008090873A1 | 2008-07-31 |
Foreign References:
| JP2013042697A | 2013-03-04 | |||
| JPH10108682A | 1998-04-28 |
Other References:
HAN JING ET AL.: "Engineered Aeromonas hydrophila for enhanced production of poly(3- hydroxybutyrate-co-3-hydroxyhexanoate) with alterable monomers composition", FEMS MICROBIOLOGY, vol. 239, no. 1, 2004, pages 195 - 201, XP004579971, ISSN: 0378-1097
KAZUNORI USHIMARU ET AL.: "An in vitro study of PHA synthase activation by PHA binding protein", ABSTRACTS OF THE ANNUAL MEETING OF THE SOCIETY FOR BIOTECHNOLOGY, JAPAN, vol. 64, 2012, pages 26
KUCHTA KENNY ET AL.: "Studies on the Influence of Phasins on Accumulation and Degradation of PHB and Nanostructure of PHB Granules in Ralstonia eutropha H16", BIOMACROMOLECULES, vol. 8, no. 2, 2007, pages 657 - 662, XP055223102, ISSN: 1525-7797
TIAN SHU-JUN ET AL.: "Effect of over- expression of phasin gene from Aeromonas hydrophila on biosynthesis of copolyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate", FEMS MICROBIOL. LETT., vol. 244, no. 1, 2005, pages 19 - 25, XP027871660, ISSN: 0378-1097
KAZUNORI USHIMARU ET AL.: "An in vitro study of PHA synthase activation by PHA binding protein", ABSTRACTS OF THE ANNUAL MEETING OF THE SOCIETY FOR BIOTECHNOLOGY, JAPAN, vol. 64, 2012, pages 26
KUCHTA KENNY ET AL.: "Studies on the Influence of Phasins on Accumulation and Degradation of PHB and Nanostructure of PHB Granules in Ralstonia eutropha H16", BIOMACROMOLECULES, vol. 8, no. 2, 2007, pages 657 - 662, XP055223102, ISSN: 1525-7797
TIAN SHU-JUN ET AL.: "Effect of over- expression of phasin gene from Aeromonas hydrophila on biosynthesis of copolyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate", FEMS MICROBIOL. LETT., vol. 244, no. 1, 2005, pages 19 - 25, XP027871660, ISSN: 0378-1097
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
Aoki 篤 (JP)
Aoki 篤 (JP)
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