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Title:
PRODUCTION METHOD FOR COPOLYMER POLYHYDROXYALKANOATE USING GENETICALLY MODIFIED STRAIN OF FATTY ACID Β-OXIDATION PATHWAY
Document Type and Number:
WIPO Patent Application WO/2014/133088
Kind Code:
A1
Abstract:
 The purpose of the present invention is to provide a method for producing poly(3-hydroxybutanoic acid-co-3-hydroxyhexanoic acid) [P(3HB-co-3HHx)] having a high 3-hydroxyhexanoic acid fraction, using a plant oil as a basic raw material. The present invention provides a method for producing P(3HB-co-3HHx) having a high 3-HHx acid fraction, using a plant oil as a basic raw material, by destroying at least one gene coding 3-hydroxyacyl-CoA dehydrogenase or at least one gene coding 2-enoyl-CoA hydratase in a chromosome of a modified Cupriavidus necator strain imparted with [P(3HB-co-3HHx)] production capability, and thus suppressing the expression of said gene.

Inventors:
FUKUI TOSHIAKI (JP)
ORITA IZUMI (JP)
Application Number:
PCT/JP2014/054905
Publication Date:
September 04, 2014
Filing Date:
February 27, 2014
Export Citation:
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Assignee:
TOKYO INST TECH (JP)
KANEKA CORP (JP)
International Classes:
C12P7/62; C12N1/21; C12N15/09
Domestic Patent References:
WO2011105379A12011-09-01
WO2011105379A12011-09-01
Foreign References:
JPH0593049A1993-04-16
JPH07265065A1995-10-17
JPH0362459B21991-09-26
Other References:
ESCAPA, I. F. ET AL.: "Disruption of beta-oxidation pathway in Pseudomonas putida KT2442 to produce new functionalized PHAs with thioester groups", APPL. MICROBIOL. BIOTECHNOL., vol. 89, 2011, pages 1583 - 1598, XP019880838
DATABASE GENBANK [online] 6 February 2013 (2013-02-06), XP055283893, retrieved from NCBI Database accession no. B0KH74
DATABASE GENBANK [online] 22 December 2012 (2012-12-22), XP055283898, retrieved from NCBI Database accession no. YP_724979
DATABASE GENBANK [online] 22 December 2012 (2012-12-22), XP055283906, retrieved from NCBI Database accession no. YP_726026
DATABASE GENBANK [online] 22 December 2012 (2012-12-22), XP055283911, retrieved from NCBI Database accession no. YP_728886
INSOMPHUN, C. ET AL.: "Modification of beta- oxidation pathway in Ralstonia eutropha for production of poly (3-hydroxybutyrate-co-3- hydroxyhexanoate) from soybean oil", J. BIOSCI. BIOENG., vol. 117, no. 2, 30 August 2013 (2013-08-30), pages 184 - 190, XP055283886
KAWASHIMA, Y. ET AL.: "Characterization and functional analyses of R-specific enoyl coenzyme A hydratases in polyhydroxyalkanoate- producing Ralstonia eutropha", APPL. ENVIRON. MICROBIOL., vol. 78, no. 2, 2012, pages 493 - 502, XP055217593
See also references of EP 2963119A4
DOI ET AL., MACROMOLECULES, vol. 28, 1995, pages 4822 - 4828
FUKUI ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 49, 1998, pages 333 - 336
BRIGHAM ET AL., J. BACTERIOL., vol. 192, 2010, pages 5454 - 5464
SAMBROOK, J. ET AL.: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY
SCHAFER ET AL., GENE, vol. 145, 1994, pages 69 - 73
QUANDT, J.; HYNES, M.P.: "Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria", GENE, vol. 127, 1993, pages 15 - 21, XP023539727, DOI: doi:10.1016/0378-1119(93)90611-6
COHEN ET AL., PROC. NATL. ACAD. SCI. USA, vol. 69, 1972, pages 2110
MOL. GEN. GENET., vol. 168, 1979, pages 111
J. MOL. BIOL., vol. 56, 1971, pages 209
J. BACTERIOL., vol. 147, 1981, pages 198
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
Aoki 篤 (JP)
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