Title:
PRODUCTION METHOD FOR DARBEPOETIN COMPOSITION, AND CULTURE METHOD FOR DARBEPOETIN-PRODUCING CELLS
Document Type and Number:
WIPO Patent Application WO/2018/159842
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for culturing darbepoetin-producing cells and thereby producing a darbepoetin composition, wherein the method increases the total amount of the darbepoetin composition produced and increases the sialic acid content of each darbepoetin molecule excreted into a culture solution by the cells. The present invention relates to a production method for a darbepoetin composition. The production method includes: a first step for culturing darbepoetin-producing cells in a culture medium to which a plant-derived protein hydrolysate has been added and obtaining a culture solution; and a second step for collecting the darbepoetin composition from the culture solution obtained in step 1.
Inventors:
TSUKAHARA MASAYOSHI (JP)
YAMAMOTO KOICHI (JP)
ISHIHARA TAKASHI (JP)
HOSONO MARETO (JP)
NAKAGAWA TAISHIRO (JP)
OKUMURA NAOFUMI (JP)
ISODA TOMOKO (JP)
YAMAMOTO KOICHI (JP)
ISHIHARA TAKASHI (JP)
HOSONO MARETO (JP)
NAKAGAWA TAISHIRO (JP)
OKUMURA NAOFUMI (JP)
ISODA TOMOKO (JP)
Application Number:
PCT/JP2018/008156
Publication Date:
September 07, 2018
Filing Date:
March 02, 2018
Export Citation:
Assignee:
KYOWA HAKKO KIRIN CO LTD (JP)
International Classes:
C12P21/02; C07K1/18; C07K1/20; C07K1/34; C07K14/505; C12N5/10
Domestic Patent References:
| WO2014035475A1 | 2014-03-06 |
Foreign References:
| JP2016538261A | 2016-12-08 | |||
| JP2011525492A | 2011-09-22 | |||
| KR20070001569A | 2007-01-04 | |||
| JPH08506023A | 1996-07-02 |
Other References:
SHUKUKOV, R. R. ET AL.: "Design of a stable cell line producing recombinant darbepoetin alpha based on CHO cells", APPL. BIOCHEM. MICROBIOL ., vol. 50, no. 9, 2014, pages 812 - 818, XP055538844
Attorney, Agent or Firm:
Eikoh Patent Firm, P.C. (JP)
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