Title:
PRODUCTION METHOD OF FRAGMENTED EXTRACELLULAR MATRIX COMPONENT
Document Type and Number:
WIPO Patent Application WO/2023/286611
Kind Code:
A1
Abstract:
One aspect of the present invention pertains to a production method of a fragmented extracellular matrix component, said production method comprising: a step for neutralizing a solution in which an extracellular matrix component is dissolved; a step for, after the neutralization step, removing the solvent by freeze-drying to give a mass containing the extracellular matrix component; a step for dispersing the mass in a solution containing a polar organic solvent and fragmenting the extracellular matrix component in the solution to give a liquid containing the fragmented extracellular matrix component; and a step for removing the liquid component from the liquid containing the fragmented extracellular matrix component.
Inventors:
YAMADA ASUKA (JP)
KITANO SHIRO (JP)
MATSUSAKI MICHIYA (JP)
KITANO SHIRO (JP)
MATSUSAKI MICHIYA (JP)
Application Number:
PCT/JP2022/026062
Publication Date:
January 19, 2023
Filing Date:
June 29, 2022
Export Citation:
Assignee:
TOPPAN INC (JP)
UNIV OSAKA (JP)
UNIV OSAKA (JP)
International Classes:
C07K1/02; C07K1/14; C07K5/09; C07K14/78; C12N1/00; C12N5/071
Domestic Patent References:
| WO2012015055A1 | 2012-02-02 | |||
| WO2019208832A1 | 2019-10-31 | |||
| WO2019189786A1 | 2019-10-03 | |||
| WO2019208831A1 | 2019-10-31 | |||
| WO2018143286A1 | 2018-08-09 |
Other References:
TOHID REZAEI TOPRAGGALEH, MOJTABA REZAZADEH VALOJERDI, LEILA MONTAZERI, HOSSEIN BAHARVAND: "A testis-derived macroporous 3D scaffold as a platform for the generation of mouse testicular organoids", BIOMATERIALS SCIENCE, R S C PUBLICATIONS, GB, vol. 7, no. 4, 26 March 2019 (2019-03-26), GB , pages 1422 - 1436, XP055703552, ISSN: 2047-4830, DOI: 10.1039/C8BM01001C
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
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