Title:
PRODUCTION METHOD OF HETEROMULTIMERIC PROTEIN, PROTEIN, NUCLEIC ACID, EXPRESSION VECTOR, TRANSFORMANT, AND PRODUCTION METHOD OF PROTEIN
Document Type and Number:
WIPO Patent Application WO/2024/071043
Kind Code:
A1
Abstract:
Provided is a novel production method of a hetero that enables the production of a heteromultimeric protein such as a bispecific antibody. The production method of the present disclosure, which is for producing a heteromultimeric protein, comprises the following two steps. A first complex-formation step for contacting two proteins to form a first complex of the two proteins, wherein: the two proteins include a first protein and a second protein; the first protein includes, from the N-terminus to the C-terminus, a first binding tag, a first cleavage domain, and a first domain in this order; the second protein includes, from the N-terminus to the C-terminus, a first binding partner capable of binding to the first binding tag, a second cleavage domain, and a second domain, in this order; the first protein and the second protein are capable of forming a dimer by binding between the first domain and the second domain; and the first binding tag binds to the first binding partner and the first domain binds to the second domain to form the first complex. A generation step for cleaving the first cleavage domain and the second cleavage domain in the first complex to generate a heterodimer of the first domain and the second domain.
Inventors:
MAKABE KOKI (JP)
Application Number:
PCT/JP2023/034748
Publication Date:
April 04, 2024
Filing Date:
September 25, 2023
Export Citation:
Assignee:
UNIV YAMAGATA NAT UNIV CORP (JP)
International Classes:
C12N15/62; C07K14/315; C07K14/33; C07K16/00; C07K16/46; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/13; C12N15/31; C12N15/63; C12P21/02; C12P21/06
Attorney, Agent or Firm:
IP LAW FIRM LEXCEED TECH (JP)
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