Title:
PRODUCTION METHOD FOR LARGE REGION DUPLICATION IN ASPERGILLUS CHROMOSOME
Document Type and Number:
WIPO Patent Application WO/2011/158623
Kind Code:
A1
Abstract:
Disclosed is a technique which is for duplicating across a wide region any large region on a chromosome of a bacterium belonging to the Aspergillus strain, and which enables the stable and systematic acquisition of a previously un-acquirable bacterium belonging to the Aspergillus strain having novel traits. Disclosed is a transformant wherein a transformation marker gene sequence is arranged on the outside of terminals (5', 3') of a region so as to sandwich an arbitrary region of a chromosome, and chromosome duplication has taken place by means of homologous recombination after double strand breakage generated in a homologous region inside the gene. Also disclosed is a method for duplicating an arbitrary region of a chromosome of a bacterium belonging to the Aspergillus strain, by cultivating said transformant.
Inventors:
TAKAHASHI Tadashi (250 Noda, Noda-sh, Chiba 01, 〒2788601, JP)
Application Number:
JP2011/062009
Publication Date:
December 22, 2011
Filing Date:
May 25, 2011
Export Citation:
Assignee:
Kikkoman Corporation (250 Noda, Noda-shi Chiba, 01, 〒2788601, JP)
キッコーマン株式会社 (〒01 千葉県野田市野田２５０番地 Chiba, 〒2788601, JP)
キッコーマン株式会社 (〒01 千葉県野田市野田２５０番地 Chiba, 〒2788601, JP)
International Classes:
C12N1/15; A23L1/238; C12N15/09
Attorney, Agent or Firm:
ABE Masahiro (Yasuda Bldg. 10F 14-2 Maebara-nishi 2-chome, Funabashi-sh, Chiba 25, 〒2740825, JP)
Claims: