Title:
PRODUCTION METHOD FOR POLYISOPRENOID, VECTOR, TRANSGENIC PLANT, PRODUCTION METHOD FOR PNEUMATIC TIRE, AND PRODUCTION METHOD FOR RUBBER PRODUCT
Document Type and Number:
WIPO Patent Application WO/2017/002818
Kind Code:
A1
Abstract:
The present invention provides a method that is for producing a polyisoprenoid, that augments the rubber-synthesis activity of rubber particles, and that makes it possible to increase production of natural rubber. The present invention is a method that is for producing a polyisoprenoid in vitro, that uses a gene that codes for a protein in the cis-prenyltransferase (CPT) family and a gene that codes for a protein in the Nogo-B receptor (NgBR) family, and that uses rubber particles to which are bound the proteins for which the genes code. In the method for producing the isoprenoid, a vector wherein a gene that codes for a protein in the CPT family and a gene that codes for a protein in the NgBR family have been bound to a promoter that promotes latex-vessel-specific gene expression is introduced into a plant and latex-vessel-specific expression of the proteins for which the genes code is induced.
Inventors:
YAMAGUCHI HARUHIKO (JP)
INOUE YUKINO (JP)
FUSHIHARA KAZUHISA (JP)
TAKAHASHI SEIJI (JP)
YAMASHITA SATOSHI (JP)
NAKAYAMA TORU (JP)
INOUE YUKINO (JP)
FUSHIHARA KAZUHISA (JP)
TAKAHASHI SEIJI (JP)
YAMASHITA SATOSHI (JP)
NAKAYAMA TORU (JP)
Application Number:
PCT/JP2016/069172
Publication Date:
January 05, 2017
Filing Date:
June 28, 2016
Export Citation:
Assignee:
SUMITOMO RUBBER IND (JP)
UNIV TOHOKU (JP)
UNIV TOHOKU (JP)
International Classes:
C12P5/02; A01H1/00; A01H5/00; C12N15/09; B60C1/00
Foreign References:
| JP2009221306A | 2009-10-01 | |||
| JP2011052146A | 2011-03-17 | |||
| JP2014227487A | 2014-12-08 | |||
| JP2015136296A | 2015-07-30 |
Other References:
ASAWATRERATANAKUL KASEM ET AL.: "Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliens is.", A KEY FACTOR PARTICIPATING IN NATURAL RUBBER BIOSYNTHESIS, EUR.J. BIOCHEM., vol. 270, no. 23, 2003, pages 4671 - 80, XP002299740
QU YANG ET AL.: "A lettuce (Lactuca sativa) homolog of human Nogo-B receptor interacts with cis-prenyltransferase and is necessary for natural rubber biosynthesis", J.BIOL.CHEM., vol. 290, no. 4, 29 January 2015 (2015-01-29), pages 1898 - 914, XP055213281
BRASHER MEGAN I. ET AL.: "A two-component enzyme complex is required for dolichol biosynthesis in tomato", PLANT J., vol. 82, no. 6, 2015, pages 903 - 14, XP055343416
HARRISON KENNETH D. ET AL.: "Nogo-B receptor is necessary for cellular dolichol biosynthesis and protein N-glycosylation", EMBO J., vol. 30, no. 12, 2011, pages 2490 - 500, XP055213277
SEIJI TAKAHASHI: "Tennen Gomu Koseisan e Hazumi o Tsukeru", JOURNAL OF THE SOCIETY FOR BIOSCIENCE AND BIOENGINEERING, vol. 93, no. 3, 2015, Japan, pages 155, XP009507894
SATOSHI YAMASHITA ET AL.: "Para Gomunoki no Gomu Ryushi ni Fukumareru Tennen Gomu Gosei Koso no Kaiseki", THE SOCIETY FOR ISOPRENOIDS, JAPAN REIKAI KOEN YOSHISHU, vol. 24, 12 September 2014 (2014-09-12), pages 17, XP009507900
QU YANG ET AL.: "A lettuce (Lactuca sativa) homolog of human Nogo-B receptor interacts with cis-prenyltransferase and is necessary for natural rubber biosynthesis", J.BIOL.CHEM., vol. 290, no. 4, 29 January 2015 (2015-01-29), pages 1898 - 914, XP055213281
BRASHER MEGAN I. ET AL.: "A two-component enzyme complex is required for dolichol biosynthesis in tomato", PLANT J., vol. 82, no. 6, 2015, pages 903 - 14, XP055343416
HARRISON KENNETH D. ET AL.: "Nogo-B receptor is necessary for cellular dolichol biosynthesis and protein N-glycosylation", EMBO J., vol. 30, no. 12, 2011, pages 2490 - 500, XP055213277
SEIJI TAKAHASHI: "Tennen Gomu Koseisan e Hazumi o Tsukeru", JOURNAL OF THE SOCIETY FOR BIOSCIENCE AND BIOENGINEERING, vol. 93, no. 3, 2015, Japan, pages 155, XP009507894
SATOSHI YAMASHITA ET AL.: "Para Gomunoki no Gomu Ryushi ni Fukumareru Tennen Gomu Gosei Koso no Kaiseki", THE SOCIETY FOR ISOPRENOIDS, JAPAN REIKAI KOEN YOSHISHU, vol. 24, 12 September 2014 (2014-09-12), pages 17, XP009507900
Attorney, Agent or Firm:
YASUTOMI & Associates (JP)
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