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Patent Searching and Data


Title:
PRODUCTION METHOD FOR PRIMITIVE GUT TUBE CELLS
Document Type and Number:
WIPO Patent Application WO/2019/208787
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method for producing primitive gut tube cells from endodermal cells that have been induced to differentiate from pluripotent stem cells, wherein the method makes it possible to produce high-quality pancreatic β cells. The present invention provides a production method for primitive gut tube (PGT) cells, the production method including a step for culturing endodermal cells that have been induced to differentiate from pluripotent stem cells, the endodermal cells being cultured in the absence of a bone morphogenetic protein (BMP) signal inhibitor.

Inventors:
IBUKI Masato (KOBE MI R&D CENTER 6-7-3, Minatojima-minamimachi, Chuo-ku, Kobe-sh, Hyogo 47, 〒6500047, JP)
MATSUTA Hirotoshi (KOBE MI R&D CENTER 6-7-3, Minatojima-minamimachi, Chuo-ku, Kobe-sh, Hyogo 47, 〒6500047, JP)
OKOCHI Hitoshi (1-21-1 Toyama, Shinjuku-k, Tokyo 55, 〒1628655, JP)
YABE Shigeharu (1-21-1 Toyama, Shinjuku-k, Tokyo 55, 〒1628655, JP)
FUKUDA Satsuki (1-21-1 Toyama, Shinjuku-k, Tokyo 55, 〒1628655, JP)
Application Number:
JP2019/017977
Publication Date:
October 31, 2019
Filing Date:
April 26, 2019
Export Citation:
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Assignee:
KANEKA CORPORATION (3-18, Nakanoshima 2-chome Kita-ku, Osaka-sh, Osaka 88, 〒5308288, JP)
NATIONAL CENTER FOR GLOBAL HEALTH AND MEDICINE (1-21-1, Toyama Shinjuku-k, Tokyo 55, 〒1628655, JP)
International Classes:
C12N5/071; C12N5/10; A61K35/37; A61K35/545; A61L27/38; A61P1/00; C12N15/09
Foreign References:
JP2009545302A2009-12-24
JP2016520291A2016-07-14
Other References:
AMERI, J. ET AL.: "Efficient generation of glucose- responsive beta cells from isolated GP2+ human pancreatic progenitors", CELL REPORTS, vol. 19, 4 April 2017 (2017-04-04), pages 36 - 49, XP055648152
MILLMAN, J. R. ET AL.: "Generation of stem cell - derived beta-cells from patients with type 1 diabetes", NATURE COMMUNICATIONS, vol. 7, 10 May 2016 (2016-05-10), XP055648467
LOO, L. S. W. ET AL.: "An arduous journey from human pluripotent stem cells to functional pancreatic beta cells", DIABETES OBES. METAB., vol. 20, January 2018 (2018-01-01), pages 3 - 13, XP055647061
Attorney, Agent or Firm:
OIKAWA Shu et al. (1-9-2, Marunouchi Chiyoda-k, Tokyo 20, 〒1006620, JP)
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