Title:
PRODUCTION METHOD FOR PROTEASE OF M23A FAMILY
Document Type and Number:
WIPO Patent Application WO/2019/142773
Kind Code:
A1
Abstract:
Provided is a method for efficiently producing a protease of the M23A family. This method is for producing a protease of the M23A family, and comprises culturing a bacterium of the genus Bacillus into which a polynucleotide encoding a pro-protein of the protease of the M23A family has been introduced, and thereby producing a mature form of the protease of the M23A family outside the cells of the bacterium of the genus Bacillus.
Inventors:
HIOKI TAKAHIRO (JP)
YAMASHITA DAICHI (JP)
TOHATA MASATOSHI (JP)
YAMASHITA DAICHI (JP)
TOHATA MASATOSHI (JP)
Application Number:
PCT/JP2019/000894
Publication Date:
July 25, 2019
Filing Date:
January 15, 2019
Export Citation:
Assignee:
KAO CORP (JP)
International Classes:
C12N15/57; C12N1/12; C12N9/48; C12R1/125
Domestic Patent References:
| WO2006068148A1 | 2006-06-29 |
Foreign References:
| JPH04108387A | 1992-04-09 | |||
| JP2003116563A | 2003-04-22 | |||
| JPH04108387A | 1992-04-09 | |||
| JP2018005193A | 2018-01-11 | |||
| JP2000210081A | 2000-08-02 | |||
| JP2011010387A | 2011-01-13 | |||
| JP2009089708A | 2009-04-30 | |||
| JPS492935B1 | 1974-01-23 | |||
| JP2006174707A | 2006-07-06 | |||
| JP2007049986A | 2007-03-01 |
Other References:
HOHMANN, HANS-PETER ET AL.: "Host Organisms: Bacillus subtilis", INDUSTRIAL BIOTECHNOLOGY: MICROORGANISMS, vol. 1, 25 November 2016 (2016-11-25) - 2017, pages 221 - 297, XP055625397
SPENCER, J. ET AL.: "Crystal structure of the LasA virulence factor from Pseudomonas aeruginosa: substrate specificity and mechanism of M23 metallopeptidases", JOURNAL OF MOLECULAR BIOLOGY, vol. 396, no. 4, 2010, pages 908 - 923, XP027234820, doi:10.1016/j.jmb.2009.12.021
LOEWY, AG ET AL.: "Purification and characterization of a novel zinc-proteinase from cultures of Aeromonas hydrophila", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 268, no. 12, 25 April 1993 (1993-04-25), pages 9071 - 9078, XP055625373
GOKCEN, A. ET AL.: "Biofilm-degrading enzymes from Lysobacter gummosus", VIRULENCE, vol. 5, no. 3, 1 April 2014 (2014-04-01), pages 378 - 387, XP055625383
DATABASE UniProt 17 February 2016 (2016-02-17), "Beta-lytic metalloendopeptidase", Database accession no. AOAOS2FCI9_9GAMM
MOLECULES, vol. 19, 2014, pages 4779 - 4790
JOURNAL OF BACTERIOLOGY, vol. 178, 1996, pages 6608 - 6617
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 95, 2003, pages 27 - 34
SCIENCE, vol. 227, 1985, pages 1435 - 1441
NUCLEIC ACIDS RES., vol. 22, 1994, pages 4673 - 4680
SHINE, J.DALGARNO, L., PROC. NATL. ACAD. SCI. USA., vol. 71, 1974, pages 1342 - 1346
JPN J GENET, vol. 60, 1985, pages 235 - 243
NUCLEIC ACIDS RES, vol. 16, 1988, pages 8732
J BACTERIOL, vol. 134, 1978, pages 318 - 329
PLASMID, vol. 18, 1987, pages 8 - 15
NATURE, vol. 390, 1997, pages 249
See also references of EP 3741856A4
SPENCER, J. ET AL.: "Crystal structure of the LasA virulence factor from Pseudomonas aeruginosa: substrate specificity and mechanism of M23 metallopeptidases", JOURNAL OF MOLECULAR BIOLOGY, vol. 396, no. 4, 2010, pages 908 - 923, XP027234820, doi:10.1016/j.jmb.2009.12.021
LOEWY, AG ET AL.: "Purification and characterization of a novel zinc-proteinase from cultures of Aeromonas hydrophila", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 268, no. 12, 25 April 1993 (1993-04-25), pages 9071 - 9078, XP055625373
GOKCEN, A. ET AL.: "Biofilm-degrading enzymes from Lysobacter gummosus", VIRULENCE, vol. 5, no. 3, 1 April 2014 (2014-04-01), pages 378 - 387, XP055625383
DATABASE UniProt 17 February 2016 (2016-02-17), "Beta-lytic metalloendopeptidase", Database accession no. AOAOS2FCI9_9GAMM
MOLECULES, vol. 19, 2014, pages 4779 - 4790
JOURNAL OF BACTERIOLOGY, vol. 178, 1996, pages 6608 - 6617
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 95, 2003, pages 27 - 34
SCIENCE, vol. 227, 1985, pages 1435 - 1441
NUCLEIC ACIDS RES., vol. 22, 1994, pages 4673 - 4680
SHINE, J.DALGARNO, L., PROC. NATL. ACAD. SCI. USA., vol. 71, 1974, pages 1342 - 1346
JPN J GENET, vol. 60, 1985, pages 235 - 243
NUCLEIC ACIDS RES, vol. 16, 1988, pages 8732
J BACTERIOL, vol. 134, 1978, pages 318 - 329
PLASMID, vol. 18, 1987, pages 8 - 15
NATURE, vol. 390, 1997, pages 249
See also references of EP 3741856A4
Attorney, Agent or Firm:
THE PATENT CORPORATE BODY ARUGA PATENT OFFICE (JP)
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