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Title:
PRODUCTION METHOD FOR PROTEIN ARRAY OR PEPTIDE ARRAY, IDENTIFICATION METHOD FOR FUNCTIONAL PROTEIN OR FUNCTIONAL PEPTIDE, PROTEIN ARRAY OR PEPTIDE ARRAY, AND IDENTIFICATION KIT FOR FUNCTIONAL PROTEIN OR FUNCTIONAL PEPTIDE
Document Type and Number:
WIPO Patent Application WO/2015/029714
Kind Code:
A1
Abstract:
Provided is a production method for a protein array or a peptide array, the method being suitable for efficient screening of functional proteins or functional peptides. The production method for a protein array or a peptide array of the present invention is characterized by having: (a) with regard to a reactor array (1) that comprises a reactor (2) having a specific aperture shape and that is provided with protein capturing molecules or peptide capturing molecules (6) on at least one portion of a wall surface (2a) and a bottom surface (2b) of the inside of the reactor (2), a step for preparing inside the reactor (2) nucleic acids and a cell-free synthesis system (9) that are fixed to a solid support; and (c) a step for using the cell-free synthesis system (9) inside the reactor (2) to synthesize proteins or peptides (7) from the nucleic acids and for fixing the proteins or peptides (7) inside the reactor (2).

Inventors:
ICHIKI TAKANORI (JP)
UENO SHINGO (JP)
OSAWA HISAO (JP)
Application Number:
PCT/JP2014/070583
Publication Date:
March 05, 2015
Filing Date:
August 05, 2014
Export Citation:
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Assignee:
UNIV TOKYO (JP)
NIKON CORP (JP)
International Classes:
C12N15/09; C07K17/14; C12M1/00; C12P21/02; G01N33/53; G01N37/00
Domestic Patent References:
WO2012026541A12012-03-01
WO2013065782A12013-05-10
WO2007000972A12007-01-04
WO2002014860A12002-02-21
WO2013063126A22013-05-02
Foreign References:
JP2004506898A2004-03-04
JP2012070654A2012-04-12
JP2005514913A2005-05-26
JP2007528718A2007-10-18
JP2013193988A2013-09-30
JP2008116218A2008-05-22
Other References:
MORIYASU J. ET AL.: "Intaglio printing of protein array pattern using coupled transcription-translation system.", EXTENDED ABSTRACTS; THE JAPAN SOCIETY OF APPLIED PHYSICS, 2010, pages 12 - 401-15A-E-7, XP008182805
MORIYASU J. ET AL.: "Intaglio printing of protein array pattern using coupled transcription-translation system [II].", EXTENDED ABSTRACTS, JAPAN SOCIETY OF APPLIED PHYSICS AND RELATED SOCIETIES, 2011, pages 12 - 238-26P-CB-14, XP008182805
See also references of EP 3040418A4
Attorney, Agent or Firm:
SHIGA Masatake et al. (JP)
Masatake Shiga (JP)
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