Title:
PRODUCTION METHOD FOR RETINAL TISSUE
Document Type and Number:
WIPO Patent Application WO/2016/063986
Kind Code:
A1
Abstract:
The present invention provides a production method for retinal cells or retinal tissue, the method including steps (1)-(3): (1) a first step for culturing human pluripotent stem cells in the absence of feeder cells in a culture medium that includes a non-differentiation maintenance factor; (2) a second step for culturing the pluripotent stem cells obtained in the first step in suspension in the presence of a sonic hedgehog signal transduction pathway activator so as to form an aggregate of cells; and (3) a third step for culturing the aggregate obtained in the second step in suspension in the presence of a BMP signal transduction pathway activator so as to obtain an aggregate that includes retinal cells or retinal tissue.
Inventors:
KUWAHARA ATSUSHI (JP)
YAMASAKI SUGURU (JP)
HIRAMINE YASUSHI (JP)
TAKAHASHI MASAYO (JP)
SASAI YOSHIKI 笹井 芳
YAMASAKI SUGURU (JP)
HIRAMINE YASUSHI (JP)
TAKAHASHI MASAYO (JP)
SASAI YOSHIKI 笹井 芳
Application Number:
PCT/JP2015/080017
Publication Date:
April 28, 2016
Filing Date:
October 23, 2015
Export Citation:
Assignee:
SUMITOMO DAINIPPON PHARMA CO LTD (JP)
RIKEN (JP)
SUMITOMO CHEMICAL CO (JP)
RIKEN (JP)
SUMITOMO CHEMICAL CO (JP)
International Classes:
C12N5/079; A61K35/44; A61L27/00; A61P27/02; C12N5/10; C12Q1/02
Domestic Patent References:
| WO2013077425A1 | 2013-05-30 |
Foreign References:
| JP2012245007A | 2012-12-13 | |||
| JP2013099345A | 2013-05-23 |
Other References:
OSAKADA F. ET AL.: "Neural Induction and Patterning in Mammalian Pluripotent Stem Cells, CNS & Neurological Disorders", DRUG TARGETS, vol. 10, no. 4, 2011, pages 419 - 432, XP009502411
MUGURUMA K. ET AL.: "Ontogeny-recapitulating generation and tissue integration of ES cell - derived Purkinje cells", NATURE NEUROSCIENCE, vol. 13, no. 10, 2010, pages 1171 - 1180, XP055059517, DOI: doi:10.1038/nn.2638
YOSHIKI SASAI: "Shinkei-kei Soshiki no Pattern Keisei ni Miru Jiko Soshikika: Sohatsu Seibutsugaku eno Chosen", BRAIN SCIENCE REVIEW, February 2014 (2014-02-01), pages 99 - 112, XP008185658
MUGURUMA K. ET AL.: "Ontogeny-recapitulating generation and tissue integration of ES cell - derived Purkinje cells", NATURE NEUROSCIENCE, vol. 13, no. 10, 2010, pages 1171 - 1180, XP055059517, DOI: doi:10.1038/nn.2638
YOSHIKI SASAI: "Shinkei-kei Soshiki no Pattern Keisei ni Miru Jiko Soshikika: Sohatsu Seibutsugaku eno Chosen", BRAIN SCIENCE REVIEW, February 2014 (2014-02-01), pages 99 - 112, XP008185658
Attorney, Agent or Firm:
TAKASHIMA, HAJIME (JP)
Takashima 1 (JP)
Takashima 1 (JP)
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