Title:
PRODUCTION OF RECOMBINANT PLASMA GELSOLIN CONTAINING A DISULFIDE BOND
Document Type and Number:
WIPO Patent Application WO1998004589
Kind Code:
A3
Abstract:
A method is provided for producing recombinant gelsolin containing a disulfide bond. The oxidized gelsolin may be produced by treating intracellular recombinant gelsolin with an oxidant, or by denaturing gelsolin and then refolding gelsolin.
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Inventors:
PEPINSKY R BLAKE (US)
MILLER STEPHAN (US)
HURKMANS KAREN (US)
WEN DINGYI (US)
MILLER STEPHAN (US)
HURKMANS KAREN (US)
WEN DINGYI (US)
Application Number:
PCT/US1997/013277
Publication Date:
March 26, 1998
Filing Date:
July 30, 1997
Export Citation:
Assignee:
BIOGEN INC (US)
PEPINSKY R BLAKE (US)
MILLER STEPHAN (US)
HURKMANS KAREN (US)
WEN DINGYI (US)
PEPINSKY R BLAKE (US)
MILLER STEPHAN (US)
HURKMANS KAREN (US)
WEN DINGYI (US)
International Classes:
C07K14/47; A61K38/00; (IPC1-7): C07K14/47; A61K38/17; C12N5/20
Domestic Patent References:
| WO1991017170A1 | 1991-11-14 |
Foreign References:
| EP0312358A2 | 1989-04-19 |
Other References:
ALLEN PG: "Functional consequences of disulfide bond formation in gelsolin.", FEBS LETT, JAN 13 1997, 401 (1) P89-94, NETHERLANDS, XP002045562
WEN D ET AL: "The plasma and cytoplasmic forms of human gelsolin differ in disulfide structure.", BIOCHEMISTRY, JUL 30 1996, 35 (30) P9700-9, UNITED STATES, XP002045563
BURTNICK L D ET AL: "The structure of horse plasma gelsolin to 2.5. A", BIOPHYSICAL JOURNAL, 70 (2 PART 2). 1996. A14., XP002045564
ITO H ET AL: "Affinity chromatography of human plasma gelsolin with polyphosphate compounds on immobilized Cibacron Blue F3GA.", J CHROMATOGR, APR 6 1990, 526 (2) P397-406, NETHERLANDS, XP002045565
WEN D ET AL: "The plasma and cytoplasmic forms of human gelsolin differ in disulfide structure.", BIOCHEMISTRY, JUL 30 1996, 35 (30) P9700-9, UNITED STATES, XP002045563
BURTNICK L D ET AL: "The structure of horse plasma gelsolin to 2.5. A", BIOPHYSICAL JOURNAL, 70 (2 PART 2). 1996. A14., XP002045564
ITO H ET AL: "Affinity chromatography of human plasma gelsolin with polyphosphate compounds on immobilized Cibacron Blue F3GA.", J CHROMATOGR, APR 6 1990, 526 (2) P397-406, NETHERLANDS, XP002045565
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