Title:
PRODUCTION AND USE OF LUMINESCENT MICROPARTICLES AND NANOPARTICLES
Document Type and Number:
WIPO Patent Application WO2001006227
Kind Code:
A3
Abstract:
The invention relates to the composition, production and use of luminescent microparticles and nanoparticles. These particles can either be used as internal standards for referencing fluorescence signals, or as markers for marking and detecting biomolecules. Luminescence dyes are incorporated, in inert form, into solid materials such that they are protected from the influence of chemical and biological compounds in aqueous sample constituents. In this incorporated form, the photophysical characteristics of the dyes (spectral characteristic, luminescence quantum efficiency, luminescence fading time and polarization) remain unaffected by sample parameters that vary. Compact inorganic materials or organic polymers are selected, in particular, as an incorporating matrix which, due to their structure, do not receive biomolecules, small neutral molecules as well as ionic compounds. In particular, the interfering influence of molecular oxygen, of an efficient luminescence quencher, on luminescence measurements is eliminated in this manner. The surface of the nanoparticles and microparticles can be provided with reactive surfaces in order to enable covalent coupling of biochemicals or to eliminate the aggregation of the particles.
Inventors:
KLIMANT INGO (DE)
Application Number:
PCT/EP2000/006832
Publication Date:
July 19, 2001
Filing Date:
July 17, 2000
Export Citation:
Assignee:
PRESENS PREC SENSING GMBH (DE)
KLIMANT INGO (DE)
KLIMANT INGO (DE)
International Classes:
B01D1/18; B01J19/00; B01D9/02; C07F13/00; C07F15/00; C09K11/06; G01N1/00; G01N33/533; G01N33/58; G01N33/96; (IPC1-7): G01N33/58; G01N33/96
Domestic Patent References:
| WO1999006821A1 | 1999-02-11 | |||
| WO1995014928A1 | 1995-06-01 | |||
| WO1996021154A1 | 1996-07-11 |
Foreign References:
| GB2132348A | 1984-07-04 |
Other References:
PATENT ABSTRACTS OF JAPAN vol. 011, no. 379 (C - 463) 10 December 1987 (1987-12-10)
HUBER, CHRISTIAN ET AL: "Optical sensor for seawater salinity", FRESENIUS' J. ANAL. CHEM. (2000), 368(2-3), 196-202, XP000974975
LIEBSCH, GREGOR ET AL: "Luminescence lifetime temperature sensing based on sol -gels and poly ( acrylonitrile )s dyed with ruthenium metal-ligand complexes", ADV. MATER. (WEINHEIM, GER.) (1999), 11(15), 1296-1299, XP000971460
HUBER, CHRISTIAN ET AL: "Optical sensor for seawater salinity", FRESENIUS' J. ANAL. CHEM. (2000), 368(2-3), 196-202, XP000974975
LIEBSCH, GREGOR ET AL: "Luminescence lifetime temperature sensing based on sol -gels and poly ( acrylonitrile )s dyed with ruthenium metal-ligand complexes", ADV. MATER. (WEINHEIM, GER.) (1999), 11(15), 1296-1299, XP000971460
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