PROMOTER VARIANT AND METHOD FOR PROTEIN PRODUCTION USING SAME

WIPO Patent Application WO/2010/064861

A3

The present invention relates to a promoter variant of Bacillus thuringiensis cry3Aa, and more particularly, to a polynucleotide having the enhanced expression rate of downstream genes by the mutation of at least one region from among the -35 region, space region, and -10 region of a Bacillus thuringiensis cry3Aa promoter, to a vector comprising the sequence of the polynucleotide, to a host cell comprising the vector, and to a method for protein production comprising (a) a step of preparing a vector comprising a cry3Aa promoter variant, (b) a step of introducing the prepared vector into a host cell, and (c) a step of recovering a target protein from the host cell.

CHOI SOO-KEUN (KR)
PAN JAE-GU (KR)
PARK SEUNG-HWAN (KR)
LEE SU-JIN (KR)

PCT/KR2009/007209

September 10, 2010

December 03, 2009

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KOREA RES INST OF BIOSCIENCE (KR)
CHOI SOO-KEUN (KR)
PAN JAE-GU (KR)
PARK SEUNG-HWAN (KR)
LEE SU-JIN (KR)

C12N15/31; C12N15/00; C12N15/09

WO2008140615A2

2008-11-20

US20020165139A1	2002-11-07
US20050112603A1	2005-05-26

BIEDENDIEK, R. ET AL.: "Plasmid System for the Intracellular Production and Purification of Affinity-Tagged Proteins in Bacillus megaterium", BIOTECHNOL. BIOENG., vol. 96, no. 3, 15 February 2007 (2007-02-15), pages 525 - 537
AGAISSE, H. ET AL.: "Structural and Functional Analysis of the Promoter Region Involved in Full Expression of the cryIIIA toxin gene of Bacillus thuringiensis", MOL. MICROBIOL., vol. 13, no. 1, 1994, pages 97 - 107

SON, Min (6th Floor City Air Tower,159-9 Samseong-dong, Gangnam-gu, Seoul 135-973, KR)

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