HOLUB, Miroslav (Vodarenska 2559, Louny, 44001, CZ)
KRUPICKOVA, Jaroslava (Chudericka 47, Litvinov, 43603, CZ)
SAROCH, Jan (Vlkov 46, Dekov, CZ-27004, CZ)
HOLUB, Miroslav (Vodarenska 2559, Louny, 44001, CZ)
KRUPICKOVA, Jaroslava (Chudericka 47, Litvinov, 43603, CZ)
| CLAIMS 1. Propolis containing active substances, whose composition is specific according to the season of harvesting and according to the origin, characterised in that it is homogenised containing dry basis of 80 to 99 % by weight, whereas the dry basis of homogenised propolis contains 0,2 to 1 ,1 % by weight of benzoic acid, 0,9 to 1 ,05 % by weight of ferulic acid, 0,15 to 0,35 % by weight of isoferulic acid, 0,038 to 0,051 % by weight of cinnamic acid, 1 ,4 to 2,22 % by weight of Galangin, 1 ,4 to 2,52 % by weight of Chrysin, 19,5 to 3,02 % by weight of isoesters, 0,25 to 1 ,26 Pinobanskin-3-acetate and maximally 0,159 % by weight of tectochrysin. 2. Method for production of propolis containing active substances of composition being specific according to the season of harvesting and according to the origin of a natural propolis, characterised in that, the batch of natural propolis is subject to freezing and subsequently to crushing, after which the first extraction of propolis is performed, at which the crushed propolis is put into ethanol, the created mixture is being macerated and subsequently is heated and stirred up, after stirring up it is let to settle until the extract is clear, after then the clear extract is sucked off and the remaining propolis paste is subject to freezing and subsequently crushing, after which the second extraction of propolis is performed, whose steps and conditions are the same as at the first extraction, the produced extract is thickened by freezing out, after which it is vacuum filtered at temperature under the freezing point and subsequently is thickened in an evaporator, through which a homogenized propolis containing dry basis of 80 to 99 % by weight is produced.. 3. Method according to the claim 2, characterised in that, the weight of ethanol for the first extraction makes a quadruple of weight of the batch of natural propolis and weight of ethanol for the second extraction makes a triple of weight of original batch of natural propolis. 4. Method according to any of the claims 2 or 3, characterised in that, the freezing of natural propolis or of propolis paste after the first extraction is performed at the temperature of - 22 °C. 5. Method according to any of the claims 2 to 4, characterised in that, the macerating lasts for a period of 10 to 12 hours at the temperature of 50 °C. 6. Method according to any of the claims 2 to 5, characterised in that, the freezing out of extract is performed at the temperature of -22 °C. 7. Method according to any of the claims 2 to 6, characterised in that, the vacuum filtration is performed at the temperature of -15 to -20 °C. |
Technical field
The invention relates to propolis containing active substances, whose composition is specific according to the season of harvesting and according to the origin and method of production of such propolis.
Background art
Propolis is a substance of orange colour for which the bees have a multiple usage. It is created of a resin which the bees enrich by secretion of their glands and the bees use it especially for disinfection of walls of hive, surface of honeycombs, etc.
Propolis is acquired by scraping off from ceiling of a hive and from frames, possibly from special gadgets which are for this purpose positioned in the hive, and it does not have a stable chemical composition.
Propolis is characteristic by very strong antibacterial and antiseptic effects, however its medical application is complicated by unstability of its chemical composition, which differs especially according to the place of harvesting and according to the season of harvesting. Therefore the processed propolis always has a different content and composition of active substances, thus also a different degree and intensity of action on the given range of health problems or quality of products produced of this propolis.
The goal of the invention is to obtain a homogenised propolis containing certain active substances in the predetermined range.
Principle of technical solution
The goal of the invention has been achieved by propolis according to the invention, whose principle consists in that it is homogenised containing dry basis 80 to 99 % by weigh, whereas the dry basis of the homogenised propolis contains 0,2 to 1 ,1 % by weight of benzoic acid, 0,9 to 1 ,05 % by weight of ferulic acid, 0,15 to 0,35 % by weight of isoferulic acid, 0,038 to 0,051 % by weight of cinnamic acid, 1 ,4 to 2,22 % by weight of Galangin, 1 ,4 to 2,52 % by weight of Chrysin, 19,5 to 3,02 % by weight of isoesters, 0,25 to 1 ,26 Pinobanskin-3-acetate and maximally 0,159 % by weight of tectochrysin.
Homogenised propolis in a standard composition of active substances in the specified range enables an industrial applicability and production of products with defined composition, thus also with defined effects.
The goal of the invention has also been achieved by a production method of propolis according to the invention, whose principle consists in that the batch of natural propolis is subject to freezing and subsequently to crushing, after which the first extraction, of propolis is performed, at which the crushed propolis is put into ethanol, the created j mixture is being, macerated and subsequently is heated and stirred up, after stirring up it is let to settle until the extract is clear, after then the clear extract is sucked off and the remaining propolis paste is subject to freezing and subsequently crushing, after which the second extraction of propolis is performed, whose steps and conditions are the same as at the first extraction, the produced extract is thickened by freezing out, after which it is vacuum filtered at temperature under the freezing point and subsequently is thickened on an evaporator, through which the homogenised propolis containing the dry basis of 80 to 99 % by ' weight is produced.
The method secures homogenisation of propolis, and as a result of this it enables an industrial applicability of the produced homogenised propolis.
The method of production is further described in details in depending patent claims.
Examples of embodiment
Natural propolis with various composition of active substances according to a season of harvesting and according to its origin is homogenised, whereas one weight part of natural propolis, for example a batch having weight of 50 kg, is frozen to -22 °C and subsequently crushed or ground using some of the known devices to small pieces, nearly powder. The freezing time depends on temperature of input raw material, its quantity and output of the freezing device. An optimum minimum time of freezing in case of the batch having weight of 50 kg seems to be at least 5 hours.
Subsequently the first extraction of propolis is performed, at which the crushed propolis is added into the kettle with stirrer and four weight parts of ethanol are added, thus 200 kg of ethanol, at the surrounding temperature. Propolis with ethanol is stirred and the created mixture is macerated usually for a period of 10 to 12 hours and subsequently is heated up to the temperature of 50 °C and is stirred up. After stirring up the mixture is let to settle until the extract is clear. Then, the liquid extract is sucked off and positioned in a freezing box, whose inner space has a temperature lower than 0°C, in advantage embodiment minus 20 to minus 22 °G. The liquid extract is let in the freezing box for at least 3 hours, optimally 5 to 6 hours or even longer, while there occurs its freezing out and coagulation of extracted propolis into small particles.
In a next step the frozen out extract is transferred into a known filtration device, such as a vacuum filtration device, whereas its temperature remains under freezing point and it usually varies in the range from -15 to -20 °C. Here, the particles of extracted propolis are separated from ethanol and subsequently are transferred into a known evaporator, in which by affect of heat its thickening occurs. The result of mentioned production procedure is homogenised propolis having a content of dry basis from 80 to 99 % by weight.
The remaining propolis paste, which remains after the first extraction, is frozen to -22 °C and subsequently is crushed in the same way as before the first extraction, then the crushed mass is transferred into a kettle with stirrer and there is added 150 kg of ethanol, i.e. 3 weight parts according to the weight of original batch (50 kg), and the second extraction of propolis is carried out, whose steps and conditions are identical as at the first extraction. After sedimentation the clear liquid extract is sucked off and is processed identically as after the first extraction.
The liquid extract, which remained in sediment, may be acquired by a subsequent filtration of sediment, whereas processing of this extract is also the same as after the first extraction and the result is, as after the first extraction, homogenised propolis with content of the dry basis of 80 to 99 % by weight.
The resultant homogenised propolis contains the below mentioned active substances in various quantities according to the season of harvesting and according to the origin, whereas the dry basis of homogenised propolis contains 0,2 to 1 ,1 % by weight of benzoic acid, 0,9 to 1,05 % by weight of ferulic acid, 0,15 to 0,35 % by weight of isoferulic acid, 0,038 to 0,051 % by weight of cinnamic acid, ,1 ,4.to 2,22 % by weight of Galangin, 1 ,4 to 2,52 % by weight of Chrysin, 19,5 to 3,02 % by weight of isoesters, 0,25 to 1 ,26 Pinobanskin-3-acetate and maximally 0,159 % by weight of tectochrysin.
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Industrial applicability {
The homogenised propolis may be industrially utilised either by crushing to powder, which may be added into various products, for example food supplements or cosmetics, or it may be dissolved in alcohol at production of propolis tincture. The propolis tincture of various concentrations of alcohol and active substances is a ' basic substance for production of new medicaments, medicine forms, food ! supplements, cosmetic preparations, etc. The homogenised propolis is applicable also in known forms, like the propolis emulsion, propolis extract,' propolis ointment or balsam, whose advantage is a repeatable composition of active substances.