Title:
PROTEASE B, AND LACTASE SOLUTION UTILIZING PROPERTIES THEREOF AND METHOD FOR MANUFACTURING SAME
Document Type and Number:
WIPO Patent Application WO/2017/159723
Kind Code:
A1
Abstract:
The protease B according to the present invention having the enzymatic properties below is a factor affecting heat stability in a lactase product, and by using this factor as an index, a lactase solution having excellent heat stability is provided. 1) The optimum temperature of the protease B is approximately 40°C. 2) The optimum pH of the protease B is approximately 8.0. 3) The protease B is stable at pH 5.0 to 8.0. 4) The protease B has high substrate specificity to FITC-casein and lactase. 5) The protease B has neutral lactase fragmentation action. 6) The molecular weight of the protease B is approximately 29,700 to 30,000 (by SDS-PAGE).
Inventors:
SUGAWARA ASAMI (JP)
YOSHIKAWA JUN (JP)
YOSHIKAWA JUN (JP)
Application Number:
PCT/JP2017/010356
Publication Date:
September 21, 2017
Filing Date:
March 15, 2017
Export Citation:
Assignee:
GODO SHUSEI KK (JP)
International Classes:
C12N15/09; C12N9/38; C12N9/50; C12N9/60; C12Q1/34; C12Q1/37
Domestic Patent References:
| WO1988006621A1 | 1988-09-07 |
Foreign References:
| JPH07508166A | 1995-09-14 | |||
| JPH08256770A | 1996-10-08 | |||
| JP2004534527A | 2004-11-18 | |||
| JP2009517061A | 2009-04-30 | |||
| JPS6018394B2 | 1985-05-10 | |||
| JP2004534527A | 2004-11-18 | |||
| JP2009517061A | 2009-04-30 | |||
| JPH05501197A | 1993-03-11 | |||
| JP2018105593A | 2018-07-05 | |||
| JPS59162833A | 1984-09-13 |
Other References:
MOEHLE C. M. ET AL.: "Protease B of the Lysosomelike Vacuole of the Yeast Sacchromyces cerevisiae Is Homologous to the Subtilisin Family of Serine Proteases", MOLECULAR AND CELLULAR BIOLOGY, vol. 7, no. 12, 1987, pages 4390 - 4399, XP009043558
DATABASE UNIPROT [online] 20 January 2016 (2016-01-20), "RecName: Full=Cerevisin; AltName: Full=Proteinase YSCB; AltName: Full=Vacuolar proteinase B; Short=PrB; Flags: Precursor", XP055601620, retrieved from NCBI Database accession no. P09232
HUSSEIN L. ET AL.: "Reduction of Lactose in Milk by Purified Lactose Produced by Kluyveromyces lactis", JOURNAL OF FOOD PROTECTION, vol. 52, no. 1, 1988, pages 30 - 34, XP55601620
KAZUHIRO HAMAGUCHI: "Yeast Neutral Lactase", MILK SCIENCE, vol. 60, no. 2, 2011, pages 99 - 104
POCH ET AL., GENE, vol. 118, no. 1, 1 September 1992 (1992-09-01), pages 55 - 63
T. MANIATIS ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY
"Food Chemicals Codex (FCC", 1 July 1996, pages: 801 - 802
See also references of EP 3431596A4
DATABASE UNIPROT [online] 20 January 2016 (2016-01-20), "RecName: Full=Cerevisin; AltName: Full=Proteinase YSCB; AltName: Full=Vacuolar proteinase B; Short=PrB; Flags: Precursor", XP055601620, retrieved from NCBI Database accession no. P09232
HUSSEIN L. ET AL.: "Reduction of Lactose in Milk by Purified Lactose Produced by Kluyveromyces lactis", JOURNAL OF FOOD PROTECTION, vol. 52, no. 1, 1988, pages 30 - 34, XP55601620
KAZUHIRO HAMAGUCHI: "Yeast Neutral Lactase", MILK SCIENCE, vol. 60, no. 2, 2011, pages 99 - 104
POCH ET AL., GENE, vol. 118, no. 1, 1 September 1992 (1992-09-01), pages 55 - 63
T. MANIATIS ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY
"Food Chemicals Codex (FCC", 1 July 1996, pages: 801 - 802
See also references of EP 3431596A4
Attorney, Agent or Firm:
ITOH, Atsushi (JP)
Download PDF: