Title:
PROTEIN ASSAY
Document Type and Number:
WIPO Patent Application WO2007028983
Kind Code:
A8
Abstract:
The invention provides a method for determining the relative amount of a sulphydryl-containing protein, such as albumin, compared with lower molecular weight thiol-containing compounds in a sample containing both sulphydryl containing protein and lower molecular weight thiol-containing compounds, comprising the step of contacting the sample with substituted or non-substituted 2.2'-dithio(bis)benzothiazole reagent (the first reagent) or a derivative thereof and determining directly or indirectly an amount of reacted thiol compounds that have reacted with the first reagent (reacted thiols) and/or thiol-containing compounds that have not reacted (unreacted thiol) with the first reagent. The method allows the discrimination between, for example, albumin and low molecular weight species such as glutathione, cysteine and homocysteine. The first reagent may be provided in a column and the sample passed through the column.
Inventors:
CHAHINE SAMIR (GB)
LIVINGSTONE CALLUM (GB)
DAVIS JAMES (GB)
LIVINGSTONE CALLUM (GB)
DAVIS JAMES (GB)
Application Number:
PCT/GB2006/003293
Publication Date:
December 13, 2007
Filing Date:
September 06, 2006
Export Citation:
Assignee:
UNIV NOTTINGHAM TRENT (GB)
CHAHINE SAMIR (GB)
LIVINGSTONE CALLUM (GB)
DAVIS JAMES (GB)
CHAHINE SAMIR (GB)
LIVINGSTONE CALLUM (GB)
DAVIS JAMES (GB)
International Classes:
G01N33/68; C07K14/76
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