Title:
PROTEIN DEAMIDATING ENZYME
Document Type and Number:
WIPO Patent Application WO/2022/168927
Kind Code:
A1
Abstract:
This invention provides a novel protein deamidating enzyme. This protein deamidating enzyme comprises a polypeptide containing an amino acid sequence shown in any of sequence numbers 1-11, or a subsequence thereof (the amino acid sequence of positions 338-515 in sequence number 1, positions 381-564 in sequence number 2, positions 122-309 in sequence number 4, positions 1-146 in sequence number 5, positions 19-235 in sequence number 6, positions 19-205 in sequence number 6, positions 10-303 in sequence number 7, positions 10-186 in sequence number 7, positions 162-426 in sequence number 9, or positions 162-341 in sequence number 9), a polypeptide which contains an amino acid sequence obtained by substituting, adding, inserting, or deleting one or multiple amino acids in the amino acid sequence in any of sequence numbers 1-11 or a subsequence thereof, and which has protein glutaminase activity, or a polypeptide which contains an amino acid sequence that has a 70% or higher sequence identity with an amino acid sequence shown in any of sequence number 1-11, or a subsequence thereof, and that has protein glutaminase activity.
Inventors:
MATSUBARA HIROTAKA (JP)
HIURA KEITA (JP)
HIURA KEITA (JP)
Application Number:
PCT/JP2022/004322
Publication Date:
August 11, 2022
Filing Date:
February 03, 2022
Export Citation:
Assignee:
AMANO ENZYME INC (JP)
International Classes:
C12N5/10; C12N1/15; C12N1/19; C12N1/21; C12N15/55; C12N15/63; C12P21/02
Other References:
DATABASE Protein 14 August 2019 (2019-08-14), ANONYMOUS: "hypothetical protein DAY19_09640 [Halobacteriovorax sp. BL9]", XP055957248, retrieved from Genbank Database accession no. RZF21941
DATABASE Protein 8 April 2019 (2019-04-08), ANONYMOUS : "hypothetical protein EP118_08880 [Halobacteriovorax sp. Y22]", XP055957253, retrieved from Genbank Database accession no. TGD47217
YAMAGUCHI S, YOKOE M: "A novel protein-deamidating enzyme from Chryseobacterium protolyticum sp. nov., a newly isolated bacterium from soil", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 66, no. 8, 1 August 2000 (2000-08-01), US , pages 3337 - 3343, XP002162600, ISSN: 0099-2240, DOI: 10.1128/AEM.66.8.3337-3343.2000
"A novel protein-deamidating enzyme from Chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil ", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 66, no. 8, 2000, pages 3337 - 43
"A novel protein glutaminase from Bacteroides helcogenes-characterization and comparison", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 104, 2020, pages 187 - 199
TATIANA A. TATSUSOVATHOMAS L. MADDEN, FEMS MICROBIOL., vol. 174, 1999, pages 247 - 250
TATIANA A. TATSUSOVATHOMAS L. MADDEN, FEMS MICROBIOL. LETT., vol. 174, 1999, pages 247 - 250
MCKENZIE, T. ET AL., PLASMID, vol. 15, no. 2, 1986, pages 93 - 103
YANGMIN GONG ET AL., JOURNAL OF BASIC MICROBIOLOGY, vol. 51, 2011, pages 666 - 672
DATABASE Protein 8 April 2019 (2019-04-08), ANONYMOUS : "hypothetical protein EP118_08880 [Halobacteriovorax sp. Y22]", XP055957253, retrieved from Genbank Database accession no. TGD47217
YAMAGUCHI S, YOKOE M: "A novel protein-deamidating enzyme from Chryseobacterium protolyticum sp. nov., a newly isolated bacterium from soil", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 66, no. 8, 1 August 2000 (2000-08-01), US , pages 3337 - 3343, XP002162600, ISSN: 0099-2240, DOI: 10.1128/AEM.66.8.3337-3343.2000
"A novel protein-deamidating enzyme from Chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil ", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 66, no. 8, 2000, pages 3337 - 43
"A novel protein glutaminase from Bacteroides helcogenes-characterization and comparison", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 104, 2020, pages 187 - 199
TATIANA A. TATSUSOVATHOMAS L. MADDEN, FEMS MICROBIOL., vol. 174, 1999, pages 247 - 250
TATIANA A. TATSUSOVATHOMAS L. MADDEN, FEMS MICROBIOL. LETT., vol. 174, 1999, pages 247 - 250
MCKENZIE, T. ET AL., PLASMID, vol. 15, no. 2, 1986, pages 93 - 103
YANGMIN GONG ET AL., JOURNAL OF BASIC MICROBIOLOGY, vol. 51, 2011, pages 666 - 672
Attorney, Agent or Firm:
TANAKA, Junya et al. (JP)
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