Title:
PROTEIN (ENZYME) FOR L-THREONINE DEHYDROGENATION, AND METHOD FOR SCREENING AND METHOD FOR PREPARING PROTEINS HAVING TARGET ACTIVITY
Document Type and Number:
WIPO Patent Application WO/2018/101403
Kind Code:
A1
Abstract:
The present invention relates to a protein having L-threonine dehydrogenase activity (including for L-threonine analysis), a method for screening said protein, a method for preparing said protein, a method for screening and a method for preparing proteins having a target activity, a method for preparing a novel synthetic protein having a target enzyme activity such as L-threonine dehydrogenase activity, and a method for analyzing L-threonine. Provided is a protein (enzyme) having L-threonine dehydrogenation activity suited for use in L-threonine analysis, the protein having higher affinity than in the past for NAD+ as well as high enzyme stability. Provided are a novel method for screening enzymes having a target activity on the basis of amino acid sequence data, irrespective of the enzyme name registered in a database, and a method for preparing enzymes screened by this method. Provided is a method for preparing proteins having a target enzyme activity, not limited to existing proteins. Provided is a method for analyzing L-threonine using newly discovered proteins having L-threonine dehydrogenation activity.
Inventors:
ASANO YASUHISA (JP)
NAKANO SHOGO (JP)
ITO SOHEI (JP)
MOTOYAMA TOMOHARU (JP)
MATSUNAGA REMI (JP)
NAKANO SHOGO (JP)
ITO SOHEI (JP)
MOTOYAMA TOMOHARU (JP)
MATSUNAGA REMI (JP)
Application Number:
PCT/JP2017/043059
Publication Date:
June 07, 2018
Filing Date:
November 30, 2017
Export Citation:
Assignee:
PUBLIC UNIV CORPORATION TOYAMA PREFECTURAL UNIV (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/06; C12P21/02; C12Q1/32; C40B30/02; C40B50/02; G06F19/22
Foreign References:
| JP2011200227A | 2011-10-13 |
Other References:
HOEHNE, MATTHIAS ET AL.: "Rational assignment of key motifs for function guides in silico enzyme identification", NAT CHEM BIOL, vol. 6, no. 11, November 2010 (2010-11-01), pages 807 - 813, XP002656961, DOI: doi:10.1038/NCHEMBIO.447
LEHMANN, MARTIN ET AL.: "From DNA sequence to improved functionality: using protein sequence comparisons to rapidly design a thermostable consensus phytase", PROTEIN ENGINEERING, vol. 13, no. 1, 2000, pages 49 - 57, XP002186780, DOI: doi:10.1093/protein/13.1.49
DATABASE PROTEIN [online] 18 March 2015 (2015-03-18), LUCAS, S ET AL.: "nucleoside-diphosphate-sugar epimerase [Thermanaerovibrio velox DSM 12556", XP055605949, retrieved from NCBI Database accession no. EHM10758.1
DATABASE PROTEIN [online] 11 December 2013 (2013-12-11), CHOVATIA, M ET AL.: "NAD-dependent epimerase/dehydratase [Thermanaerovibrio acidaminovorans DSM 6589", XP055605951, retrieved from NCBI Database accession no. ACZ19867.1
DATABASE Protein [online] 2 May 2010 (2010-05-02), GHAI, R ET AL.: "Definition: NAD dependent epimerase/dehydratase family [uncultured archaeon MedDCM-OCT-S05-C57", XP055605952, retrieved from NCBI Database accession no. ADD93128.1
DATABASE PROTEIN [online] 31 January 2014 (2014-01-31), HAWARD, J. ET AL.: "NAD-dependent epimerase/dehydratase [Collimonas fungivorans Ter331", XP055605957, retrieved from NCBI Database accession no. AEK62377.1
YAGASAKI MAKOTO: "Bioinformatics and fermentation process development", SOCIETY OF CHEMICAL ENGINEERRS, vol. 42, 6 August 2010 (2010-08-06)
MORLEY, L, KRISTA ET AL.: "Improving enzyme properties: when are closer mutations better?", TRENDS IN BIOTECHNOLOGY, vol. 23, no. 5, May 2005 (2005-05-01), pages 231 - 237, XP004872218
LEHMANN, MARTIN ET AL.: "From DNA sequence to improved functionality: using protein sequence comparisons to rapidly design a thermostable consensus phytase", PROTEIN ENGINEERING, vol. 13, no. 1, 2000, pages 49 - 57, XP002186780, DOI: doi:10.1093/protein/13.1.49
DATABASE PROTEIN [online] 18 March 2015 (2015-03-18), LUCAS, S ET AL.: "nucleoside-diphosphate-sugar epimerase [Thermanaerovibrio velox DSM 12556", XP055605949, retrieved from NCBI Database accession no. EHM10758.1
DATABASE PROTEIN [online] 11 December 2013 (2013-12-11), CHOVATIA, M ET AL.: "NAD-dependent epimerase/dehydratase [Thermanaerovibrio acidaminovorans DSM 6589", XP055605951, retrieved from NCBI Database accession no. ACZ19867.1
DATABASE Protein [online] 2 May 2010 (2010-05-02), GHAI, R ET AL.: "Definition: NAD dependent epimerase/dehydratase family [uncultured archaeon MedDCM-OCT-S05-C57", XP055605952, retrieved from NCBI Database accession no. ADD93128.1
DATABASE PROTEIN [online] 31 January 2014 (2014-01-31), HAWARD, J. ET AL.: "NAD-dependent epimerase/dehydratase [Collimonas fungivorans Ter331", XP055605957, retrieved from NCBI Database accession no. AEK62377.1
YAGASAKI MAKOTO: "Bioinformatics and fermentation process development", SOCIETY OF CHEMICAL ENGINEERRS, vol. 42, 6 August 2010 (2010-08-06)
MORLEY, L, KRISTA ET AL.: "Improving enzyme properties: when are closer mutations better?", TRENDS IN BIOTECHNOLOGY, vol. 23, no. 5, May 2005 (2005-05-01), pages 231 - 237, XP004872218
Attorney, Agent or Firm:
SIKS & CO. (JP)
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