PROTEIN HAVING EFFECT OF IMPROVING STRINGINESS OF FERMENTED MILK, AND FERMENTED MILK USING SAME AND METHOD FOR PRODUCING SAME

WIPO Patent Application WO/2022/080246

A1

At least one protein selected from the group consisting of proteins of (a)-(d). (a) A protein that has an amino acid sequence indicated by SEQ ID NO. 1. (b) A protein that has an amino acid sequence having at least one amino acid substitution, deletion, insertion, and/or addition in the amino acid sequence indicated by SEQ ID NO. 1, except for tyrosine at position 40, and that has an effect of improving stringiness of fermented milk. (c) A protein that has an amino acid sequence having an identity of 80% or more with the amino acid sequence indicated by SEQ ID NO. 1, that has tyrosine as an amino acid corresponding to that at position 40 in the amino acid sequence indicated by SEQ ID NO. 1, and that has an effect of improving stringiness of fermented milk. (d) A protein that has an amino acid sequence encoded by DNA that hybridizes, under a strict condition, with a complementary strand DNA having a nucleotide sequence indicated by SEQ ID NO. 2, that has tyrosine as an amino acid corresponding to the amino acid at position 40 in the amino acid sequence indicated by SEQ ID NO. 1, and that has an effect of improving stringiness of fermented milk.

FUJIWARA SHIN (JP)
HONME YOSHIKO (JP)
SASAKI YASUKO (JP)
KUDO HIROMU (JP)

PCT/JP2021/037285

April 21, 2022

October 08, 2021

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MEIJI CO LTD (JP)

C12N15/52; A23C9/123; A23C9/137; A23J3/20; A23L29/269; C07K14/335; C12N1/20; C12N1/21; C12N15/31; C12N15/63; C12P19/04; C12Q1/04

JP2005278638A	2005-10-13
JP2006520590A	2006-09-14

MAKINO, SEIYA ET AL.: "Application of exopolysaccharides (EPS) produced from Lactobacillus delbrueckii ss. bulgaricus, and studies on increasing the production of EPS", JAPANESE JOURNAL OF LACTIC ACID BACTERIA, vol. 24, no. 1, 2013, pages 10 - 17, XP055586897
PEI HAO, HUAJUN ZHENG, YAO YU, GUOHUI DING, WENYI GU, SHUTING CHEN, ZHONGHAO YU, SHUANGXI REN, MUNEHIRO ODA, TOMONOBU KONNO, SHENG: "Complete Sequencing and Pan-Genomic Analysis of Lactobacillus delbrueckii subsp. bulgaricus Reveal Its Genetic Basis for Industrial Yogurt Production", PLOS ONE, vol. 6, no. 1, pages e15964, XP055287606, DOI: 10.1371/journal.pone.0015964
DATABASE PROTEIN 2 April 2021 (2021-04-02), ANONYMOUS : " tyrosine-protein kinase [Lactobacillus delbrueckii subsp. bulgaricus]", XP055921171, retrieved from NCBI Database accession no. BCT69955
FUJISAWA, MINORI : "The effects of eps-gene deletion on the growth and EPS production in Lactobacillus delbrueckii subsp. bulgaricus OLL1073R-1", JAPANESE JOURNAL OF LACTIC ACID BACTERIA, vol. 31, no. 3, 1 January 2020 (2020-01-01), JP , pages 156, XP009535803, ISSN: 1343-327X

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