Title:
PROTEIN IMMOBILIZATION METHOD AND QUANTIFICATION METHOD
Document Type and Number:
WIPO Patent Application WO/2005/070968
Kind Code:
A1
Abstract:
It is intended to provide a method of immobilizing a protein on a solid phase characterized by comprising bringing the protein into contact with the solid phase having hydrophobic surface in the presence of a lower alcohol, a halogenocarboxylic acid and/or a long-chain alkyl sulfate; a liquid reagent for the immobilization to be used in this method; a method of quantifying a protein characterized by comprising bringing a solid phase carrying the protein immobilized by the immobilization method as described above into contact with a protein-staining solution and quantifying the protein depending on the degree of color development thus induced; and an immunoblotting method characterized by using a solid phase having a protein immobilized thereon by the immobilization method as described above. Moreover, it is intended to provide a method of detecting abnormal PrP and a method of judging BSE which are superior in quickness and accuracy to the existing methods.
Inventors:
KOHNO NAOYUKI (JP)
UEMORI HITOSHI (JP)
NISHIBU TAKAHIRO (JP)
HIRAYASU KAZUNARI (JP)
KOBAYASHI YOSHITERU (JP)
UEMORI HITOSHI (JP)
NISHIBU TAKAHIRO (JP)
HIRAYASU KAZUNARI (JP)
KOBAYASHI YOSHITERU (JP)
Application Number:
PCT/JP2004/000504
Publication Date:
August 04, 2005
Filing Date:
January 21, 2004
Export Citation:
Assignee:
WAKO PURE CHEM IND LTD (JP)
KOHNO NAOYUKI (JP)
UEMORI HITOSHI (JP)
NISHIBU TAKAHIRO (JP)
HIRAYASU KAZUNARI (JP)
KOBAYASHI YOSHITERU (JP)
KOHNO NAOYUKI (JP)
UEMORI HITOSHI (JP)
NISHIBU TAKAHIRO (JP)
HIRAYASU KAZUNARI (JP)
KOBAYASHI YOSHITERU (JP)
International Classes:
C07K17/02; G01N33/53; G01N33/68; (IPC1-7): C07K17/02; G01N33/53
Domestic Patent References:
| WO2002057793A2 | 2002-07-25 |
Foreign References:
| JPS58205855A | 1983-11-30 | |||
| JP2002236127A | 2002-08-23 |
Other References:
GONCALVES C A, ET AL: "Electrotransfer of fixed phosphoproteins from pieces of dried polyacrylamide gel to small disks of nitrocellulose nylon or polyvinylidene difluoride", ELECTROPHORESIS, vol. 14, no. 8, 1993, pages 789 - 793, XP002976461
JACOBSON G, ET AL: "Important parameters in semi-dry electrophoretic transfer", ELECTROPHORESIS, vol. 11, no. 1, 1990, pages 46 - 52, XP002976462
SHEFFIELD J B, ET AL: "A solid-phase method for the quantitation of protein in the presence of sodium dodecyl sulfate and other interfering substances", ANALYTICAL BIOCHEMISTRY, vol. 166, no. 1, 1987, pages 49 - 54, XP002976463
YAMAGUCHI K, ET AL: "Immunostaining method of isoferritins separated by isoelectric focusing on cellulose acetate membrane", JAPANESE JOURNAL OF ELECTROPHORESIS, vol. 35, no. 4, 1991, pages 275 - 277, XP002976464
JACOBSON G, ET AL: "Important parameters in semi-dry electrophoretic transfer", ELECTROPHORESIS, vol. 11, no. 1, 1990, pages 46 - 52, XP002976462
SHEFFIELD J B, ET AL: "A solid-phase method for the quantitation of protein in the presence of sodium dodecyl sulfate and other interfering substances", ANALYTICAL BIOCHEMISTRY, vol. 166, no. 1, 1987, pages 49 - 54, XP002976463
YAMAGUCHI K, ET AL: "Immunostaining method of isoferritins separated by isoelectric focusing on cellulose acetate membrane", JAPANESE JOURNAL OF ELECTROPHORESIS, vol. 35, no. 4, 1991, pages 275 - 277, XP002976464
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