Title:
PROTOCOL FOR IDENTIFYING AND ISOLATING ANTIGEN-SPECIFIC B CELLS AND PRODUCING ANTIBODIES TO DESIRED ANTIGENS
Document Type and Number:
WIPO Patent Application WO/2014/146074
Kind Code:
A3
Abstract:
Methods of identifying antigen-specific antibody-secreting and antibody-forming cells, such as antigen-specific B cells, and methods for cloning the antigen-specific antibody sequences of the antibody produced by these cells are provided. In particular, the methods include enriching B cells for antigen-specific B cells, culturing the antigen-specific B cells to generate clonal B cell populations, detecting clonal B cells that produce a single antigen-specific antibody, optionally screening the clonal B cell populations for functional activity, staining and sorting the cells to isolate the antigen-specific B cells, sequencing the nucleic acids encoding the antigen-specific antibody sequences, expressing the sequences to produce an antibody, isolating the antibody and screening the antibody for antigen recognition. The methods provide improved enrichment and selection of antigen-specific antibody-secreting and antibody-forming cells, which enhances recovery of antigen-specific antibodies.
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Inventors:
ALLISON DANIEL S (US)
DUTZAR BENJAMIN H (US)
GARCIA-MARTINEZ LEON F (US)
ANDERSON KATIE (US)
OJALA ETHAN W (US)
LATHAM JOHN A (US)
BILLGREN JENS (US)
JENSEN ANNE ELISABETH CARVALHO (US)
DUTZAR BENJAMIN H (US)
GARCIA-MARTINEZ LEON F (US)
ANDERSON KATIE (US)
OJALA ETHAN W (US)
LATHAM JOHN A (US)
BILLGREN JENS (US)
JENSEN ANNE ELISABETH CARVALHO (US)
Application Number:
PCT/US2014/030994
Publication Date:
November 06, 2014
Filing Date:
March 18, 2014
Export Citation:
Assignee:
ALDER BIOPHARMACEUTICALS INC (US)
International Classes:
G01N33/50
Domestic Patent References:
| WO1990000625A1 | 1990-01-25 |
Foreign References:
| US20120141982A1 | 2012-06-07 | |||
| US20050287607A1 | 2005-12-29 | |||
| US5213960A | 1993-05-25 |
Other References:
KODITUWAKKU ET AL.: "Isolation of antigen-specific B cells.", IMMUNOL CELL BIOL., vol. 81, 1 June 2003 (2003-06-01), pages 163 - 170, XP002499888
Attorney, Agent or Firm:
TESKIN, Robin, L. et al. (P.C.Intellecutal Property Department,2318 Mill Road, Suite 110, Alexandria Virginia, US)
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