Title:
PROXIMITY-BASED SORTASE-MEDIATED PROTEIN PURIFICATION AND LIGATION
Document Type and Number:
WIPO Patent Application WO/2018/053180
Kind Code:
A3
Abstract:
The invention relates to proximity-based sortase-mediated protein purification and ligation. Specifically, the invention relates to techniques that links protein expression/purification with conjugation to therapeutic agents, imaging agents, or linkers.
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Inventors:
TSOURKAS ANDREW (US)
ALTUN BURCIN (US)
WANG HEJIA HENRY (US)
YU FEIFAN (US)
ALTUN BURCIN (US)
WANG HEJIA HENRY (US)
YU FEIFAN (US)
Application Number:
PCT/US2017/051636
Publication Date:
May 23, 2019
Filing Date:
September 14, 2017
Export Citation:
Assignee:
UNIV PENNSYLVANIA (US)
International Classes:
C12P21/00; C07K16/46; G01N33/58
Foreign References:
| US20160032346A1 | 2016-02-04 |
Other References:
BEERLI ET AL.: "Sortase Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody Drug Conjugates with High In Vitro and In Vivo Potency", PLOS ONE, vol. 10, no. 7, 1 July 2015 (2015-07-01), pages e0131177, XP002756397, DOI: doi:10.1371/journal.pone.0131177
FERNANDEZ-SUAREZ ET AL.: "Protein-Protein Interaction Detection In Vitro and in Cells by Proximity Biotinylation", J AM CHEM SOC., vol. 130, no. 29, 23 July 2008 (2008-07-23), pages 9251 - 9253, XP002724821, DOI: doi:10.1021/JA801445P
FERNANDEZ-SUAREZ ET AL.: "Protein-Protein Interaction Detection In Vitro and in Cells by Proximity Biotinylation", J AM CHEM SOC., vol. 130, no. 29, 23 July 2008 (2008-07-23), pages 9251 - 9253, XP002724821, DOI: doi:10.1021/JA801445P
Attorney, Agent or Firm:
COHEN, Mark et al. (US)
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