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Title:
PURIFICATION METHOD FOR PRIMARY TREATMENT OF CHO CELL CULTURE MEDIUM
Document Type and Number:
WIPO Patent Application WO/2018/053797
Kind Code:
A1
Abstract:
A purification method for a primary treatment of a CHO cell culture medium is provided, comprising the following steps: 1) harvesting a CHO cell culture medium; 2) adding a flocculant, adjusting a pH value and stirring; and 3) clarifying the CHO cell culture medium from step 2). The purification method utilizes a combination method of chitosan, polyaluminum chloride and pH to flocculate cells, cell debris, HCP, DNA and other impurities in the cell culture medium. In addition, the residual chitosan and polyaluminum chloride can be further removed in a downstream process, which can the amount of final chitosan residue to less than 1 ppm.

Inventors:
YAN CHENGWEI (CN)
YANG BIN (CN)
SUEN WEN-CHEN (CN)
JIANG JINLONG (CN)
YAN XU (CN)
DENG CHONGFEI (CN)
Application Number:
PCT/CN2016/099851
Publication Date:
March 29, 2018
Filing Date:
September 23, 2016
Export Citation:
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Assignee:
SUNSHINE LAKE PHARMA CO LTD (CN)
International Classes:
C12N5/18; C07K1/30
Domestic Patent References:
WO2015163783A12015-10-29
Foreign References:
CN105777897A2016-07-20
CN103275877A2013-09-04
CN101591625A2009-12-02
US20090162919A12009-06-25
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