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Title:
PURIFICATION OF A TRIPLE HELIX FORMATION WITH AN IPURIFICATION OF A TRIPLE HELIX FORMATION WITH AN IMMOBILIZED OLIGONUCLEOTIDE MMOBILIZED OLIGONUCLEOTIDE
Document Type and Number:
WIPO Patent Application WO1996018744
Kind Code:
A3
Abstract:
A novel method for purifying double stranded (ds) DNA comprises passing a mixt. contg. the DNA in soln. over a support to which an oligonucleotide (ON) is covalently attached, where the ON is able to form a triple helix by hybridisation with a specific sequence (I) present in the DNA.

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Inventors:
CROUZET JOEL (FR)
SCHERMAN DANIEL (FR)
WILS PIERRE (FR)
Application Number:
PCT/FR1995/001468
Publication Date:
August 29, 1996
Filing Date:
November 08, 1995
Export Citation:
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Assignee:
RHONE POULENC RORER SA (FR)
CROUZET JOEL (FR)
SCHERMAN DANIEL (FR)
WILS PIERRE (FR)
International Classes:
C07H21/04; C12N15/09; C12Q1/68; C12Q1/6839; (IPC1-7): C12Q1/68
Domestic Patent References:
WO1993000352A11993-01-07
WO1992011390A11992-07-09
WO1992013963A11992-08-20
WO1992009705A11992-06-11
WO1994017086A11994-08-04
Foreign References:
US4766072A1988-08-23
Other References:
AUSUBEL ET AL.: "CURRENT PROTOCOLS IN MOLECULAR BIOLOGY", 1994, WILEY AND SONS, INC., NEW YORK, XP002005961
AUSUBEL ET AL.: "CURRENT PROTOCOLS IN MOLECULAR BIOLOGY", 1991, WILEY AND SONS, INC., NEW YORK, XP002005962
SAMBROOK ET AL.: "Laboratory Manual", 1987, COLD SPRING HARBOUR PRESS, NEW YORK, XP002005963
HE ET AL.: "An improved and rapid procedure for isolating RNA free E. coli plasmid DNA", GENE ANAL. TECH. APPL., vol. 8, no. 3, 1991, NEW YORK, pages 107 - 110, XP000573842
ITO ET AL.: "Sequence-specific DNA purification by triplex affinity capture", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 89, WASHINGTON US, pages 495 - 498, XP002000128
H. JARRET: "AFFINITY CHROMATOGRAPHY WITH NUCLEIC ACID POLYMERS.", J CHROMATOGR BIOMED APPL 618 (1-2). 1993. 315-339., XP002000129
KIESSLING ET AL.: "Flanking sequence effects within the pyrimidine Triple Helix motif characterized by affininty cleaving", BIOCHEMISTRY, vol. 31, 1992, EASTON, PA US, pages 2829 - 2834, XP002000130
DUVAL ET AL.: "Specific inhibition of transcription by triple helix forming oligonucleotides", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 89, 1992, WASHINGTON US, pages 504 - 508, XP002000131
JAYASENA ET AL.: "Oligonucleotide directed triple helix formation at adjacent oligopurine and olygopyrimidine DNA tracts by alternate strand recognition", NUCLEIC ACIDS RESEARCH, vol. 20, no. 20, 1992, OXFORD GB, pages 5279 - 5288, XP002000132
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