Title:
PURINES AND PYRIMIDINES LINKED TO A QUENCHER
Document Type and Number:
WIPO Patent Application WO1999064431
Kind Code:
A3
Abstract:
Compounds are described comprising purines substituted at the C-8 position and pyrimidines substituted at the C-4 or C-5 position with a linker and quencher. These compounds when incorporated into hairpin oligonucleotides quench the fluorescence of fluorophores linked to the 5'-terminus of the oligonucleotide. These nucleotide-quencher compounds are also easy to incorporate into oligonucleotides using conventional, automated, oligonucleotide synthetic techniques.
Inventors:
NARDONE GLENN (US)
NAZARENKO IRENA (US)
BOAL JILA (US)
NAZARENKO IRENA (US)
BOAL JILA (US)
Application Number:
PCT/US1999/013107
Publication Date:
June 15, 2000
Filing Date:
June 10, 1999
Export Citation:
Assignee:
INTERGEN COMPANY (US)
NARDONE GLENN (US)
NAZARENKO IRENA (US)
BOAL JILA (US)
NARDONE GLENN (US)
NAZARENKO IRENA (US)
BOAL JILA (US)
International Classes:
C07H19/06; C07H19/10; C07H19/16; C07H19/20; C07H21/00; (IPC1-7): C07H19/10; C07H19/20; C07H21/00
Domestic Patent References:
| WO1998002449A1 | 1998-01-22 |
Foreign References:
| US4948882A | 1990-08-14 | |||
| EP0267996A1 | 1988-05-25 |
Other References:
M. R. MAURIZI ET AL.: "Distances between active site probes in glutamine synthetase from Escherichia Coli: fluorescence energy transfer in free and stacked dodecamers", BIOCHEMISTRY, vol. 25, no. 1, 1986, pages 141 - 151, XP002131927
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