Title:
PURITY ANALYSIS METHOD FOR EXTRACELLULAR VESICLES, USING SIZE EXCLUSION CHROMATOGRAPHY
Document Type and Number:
WIPO Patent Application WO/2019/066485
Kind Code:
A1
Abstract:
The present invention relates to a purity analysis method for extracellular vesicles, using size exclusion chromatography, and more specifically the present invention relates to a method of using the size-specific separation ability of size exclusion chromatography and spectroscopy, that is single or in which two or more kinds are combined, in order to quantify, from various samples, the volume of extracellular vesicles and various contaminants, simultaneously, and thereby analyze the purity, total amount and yield of extracellular vesicles. The purity analysis method for extracellular vesicles, according to the present invention, has the benefit of being capable of effective analysis whilst preserving the form or properties of the extracellular vesicles. Also, the purity analysis method for extracellular vesicles, according to the present invention, makes it possible to rapidly determine, simultaneously, the purity, total amount and yield of extracellular vesicles that have been separated using a conventional method, and can be utilized in, for example, disease diagnosis, disease treatment, multi-omics research and research into the properties of extracellular vesicles which use separated extracellular vesicles. In addition, the purity analysis method according to the present invention can also be applied to the development of a new separation method.
Inventors:
GHO YONG SONG (KR)
LEE CHANG JIN (KR)
PARK HYUN TAEK (KR)
LEE CHANG JIN (KR)
PARK HYUN TAEK (KR)
Application Number:
PCT/KR2018/011415
Publication Date:
April 04, 2019
Filing Date:
September 27, 2018
Export Citation:
Assignee:
ROSETTA EXOSOME (KR)
POSTECH ACAD IND FOUND (KR)
POSTECH ACAD IND FOUND (KR)
International Classes:
G01N30/88; B01D15/34; G01N21/31
Foreign References:
| KR20140139122A | 2014-12-04 | |||
| US6002986A | 1999-12-14 |
Other References:
KOTMAKCI, M.: "Exosome isolation: Is there an optimal method with regard to diagnosis or treatment?", NOVEL IMPLICATIONS OF EXOSOMES IN DIAGNOSIS AND TREATMENT OF CANCER AND INFECTIOUS DISEASES, 12 July 2017 (2017-07-12), pages 163 - 182, XP055586020
BOND, M. D.: "Evaluation of a dual-wavelength size exclusion HPLC method with improved sensitivity to detect protein aggregates and its use to better characterize degradation pathways of an IgG1 monoclonal antibody", JOURNAL
CASTLEDINE, J. B.: "Assessment of chromatographic peak purity by means of multi-wavelength detection and correlation-based algorithms", JOURNAL OF - CHROMATOGRAPHY, vol. 592, 1992, pages 27 - 36, XP026503508, DOI: doi:10.1016/0021-9673(92)85069-6
CASTLEDINE, J. B. ET AL.: "A multiwavelength approach to the selection of absorbance ratios for the assessment of chromatographic peak purity", JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 8 September 1991 (1991-09-08), pages 619 - 624, XP055586021
BOND, M. D.: "Evaluation of a dual-wavelength size exclusion HPLC method with improved sensitivity to detect protein aggregates and its use to better characterize degradation pathways of an IgG1 monoclonal antibody", JOURNAL
CASTLEDINE, J. B.: "Assessment of chromatographic peak purity by means of multi-wavelength detection and correlation-based algorithms", JOURNAL OF - CHROMATOGRAPHY, vol. 592, 1992, pages 27 - 36, XP026503508, DOI: doi:10.1016/0021-9673(92)85069-6
CASTLEDINE, J. B. ET AL.: "A multiwavelength approach to the selection of absorbance ratios for the assessment of chromatographic peak purity", JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 8 September 1991 (1991-09-08), pages 619 - 624, XP055586021
Attorney, Agent or Firm:
ERUUM & LEEON INTELLECTUAL PROPERTY LAW FIRM (KR)
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