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Title:
PUROMYCIN LINKER AND APPLICATION THEREOF IN SYNTHESIS OF IN-VITRO NUCLEIC ACID DISPLAYED PEPTIDE
Document Type and Number:
WIPO Patent Application WO/2022/087804
Kind Code:
A1
Abstract:
Provided are a puromycin linker and an application thereof in synthesis of an in-vitro nucleic acid displayed peptide. The puromycin linker is a modified and unbranched single-stranded DNA; the single-stranded DNA comprises a first stretch of nucleotides and a second stretch of nucleotides; the 5' end of the first stretch of nucleotides is linked to the 5' end of the second stretch of nucleotides to form a linker structure containing two 3' ends; puromycin is modified at the 3' end of the first stretch of nucleotides; and the second stretch of nucleotides comprise, in an order of 5' to 3', an mRNA linking site and a reverse transcription site. A synthesis method for the puromycin linker is simple; the efficiency is up to 53%; the efficiency of obtaining key raw materials is greatly improved; and the system efficiency is further improved and synthesis costs of a cDNA-protein are reduced.

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Inventors:
SANG GUOQIN (CN)
JIAO SHAOZHUO (CN)
XIE YINGYING (CN)
XU MENG (CN)
LI ZONGWEN (CN)
Application Number:
PCT/CN2020/123835
Publication Date:
May 05, 2022
Filing Date:
October 27, 2020
Export Citation:
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Assignee:
BEIJING SEEKGENE BIOSCIENCES CO LTD (CN)
International Classes:
C12N15/10; C07K17/00; C12Q1/68
Foreign References:
US10294472B22019-05-21
CN102227638A2011-10-26
US20190085322A12019-03-21
US20180100146A12018-04-12
CN104774923A2015-07-15
Other References:
MOCHIZUKI, Y. ET AL.: "A versatile puromycin-linker using cnvK for high-throughput in vitro selection by cDNA display", JOURNAL OF BIOTECHNOLOGY, vol. 212, 28 August 2015 (2015-08-28), pages 174 - 180, XP055769425
Attorney, Agent or Firm:
BEIJING SANYOU INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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