PYRROLIDINE AND PIPERIDINE COMPOUNDS

FIELD

[0001] The present technology relates to pyrrolidine and piperidine compounds, stereoisomers, and pharmaceutically acceptable salts thereof having inhibitory activity on fibroblast activation protein (FAP). Processes for the preparation, pharmaceutical compositions, and methods of use are also provided.

BACKGROUND

[0002] The fibroblast activation protein (FAP) is a homodimeric type II integral membrane serine protease belonging to the dipeptidyl peptidase-4 (dipeptidyl peptidase IV, DPP4) group. This protein group includes other subtype proteins such as DPP7, 8, 9, etc. FAP and DPP4 have about 50% amino acid sequence similarity. However, unlike DPP4 which is expressed in most biological tissues, FAP is expressed in tissue remodeling sites including, but not limited to, malignant tumor, fibrosis, atherosclerosis, and arthritis.

[0003] FAP, like DPP4, has dipeptidyl peptidase activities capable of cleaving the post-proline bond at two or more residues from the N-terminus but, unlike DPP4, FAP also has specific enzymatic activities of endopeptidase activities capable of cleaving the glycine-proline bond present in the substrate (Aertgeerts et al., J. Biol. Chem., 2005, 280, 19441). Moreover, FAP has collagenase activities capable of degrading gelatin and type 1 collagen (CN - 1) (Fuming Zi et al., Molecular Medicine Reports, November 2015, 3203).

[0004] FAP is expressed where tissue remodeling is in progress, for example, liver fibrosis (Levy et al., Hepatol., 1999, 29: 1768). It was found that patients suffering from fibrosis as a result of alcohol abuse or viral infection have a great quantity of FAP expressed in cells associated with excessive scarring and liver diseases. As an example, it was reported that the expression of FAP increases in activated hepatic stellate cells (hereinafter, “aHSC”) and myofibroblasts, which play important roles in liver fibrosis or liver fibrosis (Levy et al., Hepatol., 1999, 29, 1768; Lay AJ et al., Front. Biosci. [Landmark Ed] 2019, 24, 1-17).

[0005] The substrates degraded by FAP include α2-antiplasmin, Sprouty-2 protein, and gelatin and type 1 collagen (CN-1). Recently, it was reported that fibroblast growth factor-21 (FGF21) which is one of non-mitogenic hepatokines is also a substrate degraded by FAP (D. R. Dunshee et al., J. Biol. Chem., 2016, 291, 5986).

[0006] Accordingly, there is a need for FAP inhibitors, which may be usefully applied for the prophylaxis and treatment of diseases. SUMMARY

[0007] Provided herein, in one aspect is a compound of Formula 1 : wherein n is 1 or 2;

R ₁ is selected from the group consisting of Formulas I, II, III, IV, and IV;

R ₂ is a hydrogen or a C _1-3 alkyl;

R ₃ is a 5- to 12-membered heteroaryl, wherein said heteroaryl contains 1 to 3 heteroatoms selected from O, N, and S and is optionally substituted by 1 to 3 Z, a 3 - to 12-membered non-aromatic heterocycle, wherein said heterocycle contains 1 to 3 heteroatoms selected from O, N, and S and is optionally substituted by 1 to 3 Z,

-NR ₄ R ₅ , -OR ₄ , -C(O)NHR ₄ , -NHC(O)(CH ₂ )mR ₄ , -NHS(O) ₂ R ₄ , -(CH ₂ ) _m CH ₂ R ₄ , or - (CH ₂ ) _m NHR ₄ , wherein m is 0 or 1; R ₄ is a phenyl substituted with 1 to 3 Z, a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2- oxo-1,2-dihydroquinolinyl, and 2- oxo-1,2,3,4-tetrahydroquinolinyl, wherein said heterocycle is optionally substituted with 1 to 3 Z, a naphthyl optionally substituted with 1 to 3 Z, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2- c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, acridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z;

R ₅ is a hydrogen or a C _1-3 alkyl; and each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted by 1 to 3 Z ¹ , C _2-6 alkenyl optionally substituted by Z ¹ , C _2-6 alkynyl optionally substituted by Z ¹ , C _1-5 alkoxy optionally substituted by 1 to 3 Z ¹ , C _1-5 alkylthio optionally substituted by Z ¹ , mono- or di-C _1-5 alkylamino optionally substituted by Z ¹ , piperazinyl optionally substituted by Z ¹ , C _1-5 alkylsulfonylamino optionally substituted by Z ¹ , C _1-5 alky1carbonylamino optionally substituted by Z ¹ , aminosulfonyl optionally substituted by Z ¹ , aminocarbonyl optionally substituted by Z ¹ , C _{1- 5} alkylaminocarbonyl optionally substituted by Z ¹ , phenyl optionally substituted by Z ¹ , phenoxy optionally substituted by Z ¹ , benzyl optionally substituted by Z ¹ , benzoyl optionally substituted by Z ¹ , phenylaminocarbonyl optionally substituted by Z ¹ , pyrazolyl optionally substituted by Z ¹ , benzoxazolyl optionally substituted by Z ¹ , C _1-5 alkoxy carbonyl optionally substituted by Z ¹ , benzyloxy optionally substituted by Z ¹ , C _1-5 alkylsulfonyl optionally substituted by Z ¹ , acetyl, morpholinyl optionally substituted by Z ¹ , -NR ₆ C(O)R ₇ , and - C(O)NR6R ₇ ; wherein each Z ¹ is independently chosen from halogen, hydroxyl, amino, C _1-5 alkylamino, cyano, acetyl, C _1-5 alkyl, C _1-5 haloalkyl. C _3-6 carbocycle, C _3-6 heterocycle, wherein said C _3-6 carbocycle and said C _3-6 heterocycle are optionally substituted with halogen, hydroxyl, C _1-5 alkyl or C _1-5 haloalkyl;

R ₆ is hydrogen or a C _1-3 alkyl group;

R ₇ is a C _1-3 alkyl group optionally substituted with phenyl, a phenyl optionally substituted with 1 to 3 Z ² , or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2- c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, acridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z ² ; and each Z ² is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, hydroxycarbonyl, C _1-5 alkyl optionally substituted by 1 to 3 Z ³ , C _2-6 alkenyl, C _2-6 alkynyl, C _{1- 5} alkoxy optionally substituted byl to 3 Z ³ , C _1-5 alkylthio, mono- or di-C _1-5 alkylamino, piperazinyl optionally substituted by Z ³ , C _1-5 alkylsulfonylamino, C _1-5 alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _1-5 alkylaminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; wherein each Z ³ is independently chosen from halogen, amino, or acetyl; or a stereoisomer or a mixture of stereoisomers, or a pharmaceutically acceptable salt thereof.

[0008] In some embodiments, R ₁ is selected from the group consisting of compounds of Formulas

I, II, III, and V:

[0009] In some embodiments, R ₁ is a compound of Formula I : In some embodiments, R ₁ is a compound of Formula II: In some embodiments, R ₁ is a compound of Formula III: In some embodiments, R ₁ is a compound of Formula

IV: In some embodiments, R ₁ is a compound of Formula V:

[0010] In some embodiments, R ₁ is a substituent selected from the group consisting of compounds of Formulas Ia-Va,

[0011] In some embodiments, R ₁ is a substituent selected from the group consisting of compounds of Formulas la, Ila, Ilia, and Va:

[0012] In some embodiments, R ₁ is a compound of Formula la: . In some embodiments, R ₁ is a compound of Formula Ila: In some embodiments, R ₁ is a compound of Formula Ilia: . In some embodiments, R ₁ is a compound of Formula

IVa: . In some embodiments, R ₁ is a compound of Formula Va:

[0013] In some embodiments, R ₂ is hydrogen. In some embodiments, R ₂ is a C _1-3 alkyl.

[0014] In some embodiments, the compound, or its stereoisomer or mixture of stereoisomers, or its pharmaceutically acceptable salt has a structure of Formula la:

In further embodiments, R ₅ is hydrogen. In other embodiments, R ₅ is a C _1-3 alkyl.

[0015] In some embodiments, the compound, or its stereoisomer or mixture of stereoisomers, or its pharmaceutically acceptable salt has a structure of Formula 1b:

[0016] In some embodiments, the compound, or its stereoisomer or mixture of stereoisomers, or its pharmaceutically acceptable salt has a structure of Formula 1c:

[0017] In some embodiments, the compound, or its stereoisomer or mixture of stereoisomers, or its pharmaceutically acceptable salt has a structure of Formula 1d: embodiments, m is 0. In other embodiments, m is 1.

[0018] In some embodiments, the compound, or its stereoisomer or mixture of stereoisomers, or its pharmaceutically acceptable salt has a structure of Formula 1e: [0019] In some embodiments, R ₃ is a substituent selected from the group consisting of -NR ₄ R ₅ , - OR ₄ , -C(O)NHR ₄ , -NHC(O)(CH ₂ ) _m R ₄ , and -NHS(O) ₂ R ₄ . In some embodiments, R ₃ is a substituent selected from the group consisting of -NR ₄ R ₅ , -OR ₄ , and -NHC(O)(CH ₂ ) _m R ₄ . In some embodiments,

R ₃ is a substituent selected from the group consisting of -NR ₄ R ₅ and -OR ₄ .

[0020] In some embodiments, R ₄ is a phenyl substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted with 1 to 3 halogens or amino, C _2-6 alkenyl, C _2-6 alkynyl, C _{1- 5} alkoxy, C _1-5 alkylthio, mono- or di-C _1-5 alkylamino, piperazinyl optionally substituted with acetyl, C _{1- 5} alkylsulfonylamino, C _1-5 alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _{1- 5} alkylaminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl. In further embodiments, R ₄ is a phenyl substituted with a substituent selected from the group consisting of piperazinyl optionally substituted with acetyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl.

[0021] In some embodiments, R ₄ is a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2- oxo-1,2- dihydroquinolinyl, and 2-oxo-1,2,3,4-tetrahydroquinolinyl, wherein said heterocycle is optionally substituted with 1 to 3 Z. In further embodiments, R ₄ is a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2- oxo- 1 ,2,3 ,4-tetrahydroquinolinyl .

[0022] In some embodiments, R ₄ is a naphthyl optionally substituted with halogen.

[0023] In some embodiments, R ₄ is a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2-c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, acridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _{1- 5} alkylsulfonyl, morpholinyl, -NR ₆ C(O)R ₇ , and -C(O)NR ₆ R ₇ . In further embodiments, R ₄ is a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzofuranyl, indolinyl, benzimidazolyl, benzothiophenyl, benzothiazolyl, furo[3,2- c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, acridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, acetyl, morpholinyl, -NR ₁ C(O)R ₇ , -C(O)NR ₆ R ₇ . and phenyl optionally substituted with halogen; wherein R ₆ is hydrogen or a C _1-3 alkyl group; and R ₇ is a C _1-3 alkyl group optionally substituted with phenyl, a phenyl, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0024] In some embodiments, R ₄ is a phenyl substituted with a substituent selected from the group consisting of piperazinyl optionally substituted with acetyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, 2,3- dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2-oxo-1,2,3,4-tetrahydroquinolinyl; a naphthyl optionally substituted with halogen; or a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzofuranyl, indolinyl, benzimidazolyl, benzothiophenyl, benzothiazolyl, furo[3,2-c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, acridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1- 5alkoxycarbonyl, benzyloxy, acetyl, morpholinyl, -N R ₆ C(O)R ₇ , -C(O)NR ₆ R ₇ . and phenyl optionally substituted with halogen; wherein R ₅ is hydrogen or a C _1-3 alkyl group; and R ₇ is a C _1-3 alkyl group optionally substituted with phenyl, a phenyl, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0025] In some embodiments, R ₄ is a phenyl substituted with phenyl; a naphthyl; or a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzofuranyl, indolinyl, benzimidazolyl, benzothiophenyl, benzothiazolyl, furo[3,2-c]pyridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, benzyloxy, acetyl, morpholinyl, -NR ₁ C(O) R ₇ , and -C(O)NR ₅ R ₇ . In further embodiments, R ₄ is a phenyl substituted with phenyl. In other embodiments, R ₄ is a naphthyl. In still other embodiments, R ₄ is a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzofuranyl, indolinyl, benzimidazolyl, benzothiophenyl, benzothiazolyl, furo[3,2-c]pyridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, benzyloxy, acetyl, morpholinyl, -NR ₆ C(O)R ₇ , and -C(O)NR ₆ R ₇ : R ₆ is hydrogen or a C _1-3 alkyl group; and R ₇ is a C _1-3 alkyl group optionally substituted with phenyl, a phenyl, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl. In further embodiments, R ₄ is quinolinyl or isoquinolinyl, each of which is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, benzyloxy, acetyl, morpholinyl, -NR ₆ C(O)R ₇ , and - C(O)N R ₆ ,R ₇ . In still further embodiments, R ₄ is quinolinyl, optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, benzyloxy, acetyl, morpholinyl, -NR ₆ C(O)R ₇ . and -C(O)NRr,R ₇ . In other embodiments, R ₄ is benzofuranyl, benzothiophenyl, or furo[3,2-c]pyridinyl, each of which is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of halogen, C _1-5 alkyl. and C _1-5 alkoxy. In further embodiments, R ₄ is benzofuranyl or benzothiophenyl, each of which is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of halogen, C _1-5 alkyl, and C _1-5 alkoxy.

[0026] In some embodiments, R ₄ is a phenyl substituted with a substituent selected from the group consisting of piperazinyl optionally substituted with acetyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, 2,3- dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2-oxo-1,2,3,4-tetrahydroquinolinyl; a naphthyl optionally substituted with halogen; or a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, quinazolinyl, thieno[2,3-d]pyrimidinyl, isoxazolyl, and acridinyl wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of cyano, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, benzyloxy, and phenyl optionally substituted with halogen. In further embodiments, R ₄ is a phenyl substituted with a substituent selected from the group consisting of piperazinyl optionally substituted with acetyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl. In other embodiments, R ₄ is a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2- dihydroquinolinyl, and 2-oxo-1,2,3,4-tetrahydroquinolinyl. In other embodiments, R ₄ is a naphthyl optionally substituted with halogen. In other embodiments, R ₄ is a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, quinazolinyl, thieno[2,3-d]pyrimidinyl, isoxazolyl, and acridinyl wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of cyano, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, benzyloxy, and phenyl optionally substituted with halogen. In further embodiments, R ₄ is quinolinyl, isoquinolinyl, or quinazolinyl, each of which is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of cyano, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, and C _1-5 alkoxy optionally substituted with 1 to 3 halogens.

[0027] In some embodiments, R ₄ is quinolinyl.

[0028] In some embodiments, R ₄ is a phenyl substituted with phenyl; a naphthyl; or a heteroaryl selected from the group consisting of quinolinyl, isoquinolinyl, benzofuranyl, and benzothiophenyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, halogen, C _1-5 alky l . and C _1-5 alkoxy. In further embodiments, R ₄ is a phenyl substituted with phenyl. In other embodiments, R ₄ is a naphthyl. In other embodiments, R ₄ is a heteroaryl selected from the group consisting of quinolinyl, isoquinolinyl, benzofuranyl, and benzothiophenyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, halogen, C _1-5 alkyl and C _1-5 alkoxy. In further embodiments, R ₄ is benzofuranyl, optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, halogen, C _1-5 alkyl, and C _1-5 alkoxy. In other embodiments, R ₄ is quinolinyl or isoquinolinyl, each of which is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, halogen, C _1-5 alkyl, and C _1-5 alkoxy.

[0029] In some embodiments, R ₄ is benzofuranyl or benzothiophenyl.

[0030] In some embodiments, R ₃ is a 3- to 12-membered non-aromatic heterocycle comprising nitrogen, wherein said heterocycle is optionally substituted by 1 to 3 Z. In some embodiments, R ₃ is a 5- to 12-membered heteroaryl optionally substituted by 1 to 3 Z. In some embodiments, R ₃ is 2-oxo- 2,3 -dihydro-1H-benzo [d]imidazol- 1 -yl .

[0031] In some embodiments, n is 1 or 2; R ₁ is a substituent selected from the group consisting of compounds of Formulas I, II, III, and V; R ₂ is hydrogen; R ₃ is a substituent selected from the group consisting of -NR ₄ R ₅ , -OR ₄ , -C(O)NHR ₄ , -NHC(O)(CH ₂ ) _m R ₄ , and -NHS(O) ₂ R ₄ ; wherein m is 0 or 1; wherein R ₄ is a phenyl substituted with a substituent selected from the group consisting of piperazinyl optionally substituted with acetyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, 2,3- dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2-oxo-1,2,3,4-tetrahydroquinolinyl; a naphthyl optionally substituted with halogen; or a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, quinazolinyl, benzofuranyl, benzothiophenyl, furo[3,2- c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, and acridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, and phenyl optionally substituted with halogen; and R ₅ is hydrogen.

[0032] In some embodiments, n is 1. In some embodiments, n is 2.

[0033] In some embodiments, the compound, or its stereoisomer or mixture of stereoisomers, or its pharmaceutically acceptable salt is selected from Table 1, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof.

[0034] Provided herein, in another aspect, is a pharmaceutical composition comprising a compound described herein, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable excipient.

[0035] Provided herein, in another aspect, is a method of inhibiting fibroblast activation protein in a mammal, comprising administering, to the mammal, a therapeutically effective amount of a compound, or its stereoisomer or mixture of stereoisomers, or its pharmaceutically acceptable salt described herein.

[0036] Provided herein, in another aspect, is a method of treating NASH in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of a compound described herein, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, or a therapeutically effective amount of a pharmaceutical composition described herein.

[0037] Provided herein, in another aspect, is a use of a compound described herein, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, for the manufacture of a medicament for the treatment of NASH.

[0038] Provided herein, in another aspect, is a compound described herein, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, for use in treating NASH.

[0039] Provided herein, in another aspect, is a composition described herein for use in treating NASH.

[0040] Provided herein, in another aspect, is a compound described herein, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, for use in inhibiting fibroblast activation protein.

[0041] Provided herein, in another aspect, is a composition described herein for use in inhibiting fibroblast activation protein.

[0042] Provided herein, in another aspect, is a method of treating a disease mediated by fibroblast activation protein in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of a compound described herein, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, or a therapeutically effective amount of a pharmaceutical composition described herein. In some embodiments, the disease mediated by fibroblast activation protein is selected from the group consisting of lipid and lipoprotein disorders, conditions and diseases which result from chronic fatty and fibrotic degeneration of organs due to accumulated lipid and specifically triglyceride accumulation and subsequent activation of profibrotic pathways, Type I or Type II Diabetes and clinical complications of Type I and Type II Diabetes, chronic intrahepatic or some forms of extrahepatic cholestatic conditions, liver fibrosis, acute intraheptic cholestatic conditions, obstructive or chronic inflammatory disorders that arise out of improper bile composition, gastrointestinal conditions with a reduced uptake of dietary fat and fat- soluble dietary vitamins, inflammatory bowel diseases, obesity and metabolic syndrome (combined conditions of dyslipidemia, diabetes and abnormally high body-mass index), persistent infections by intracellular bacteria or parasitic protozoae, non-malignant hyperproliferative disorders, malignant hyperproliferative disorders, colon adenocarcinoma and hepatocellular carcinoma in particular, liver steatosis and associated syndromes, Hepatitis B infection, Hepatitis C infection and/or of cholestatic and fibrotic effects that are associated with alcohol-induced cirrhosis or with viral-borne forms of hepatitis, liver failure or liver malfunction as an outcome of chronic liver diseases or of surgical liver resection, acute myocardial infarction, acute stroke, thrombosis which occurs as an endpoint of chronic obstructive atherosclerosis, osteoarthritis, rheumatoid arthritis, psoriasis, and cerebral infarction, individually or any combination thereof.

[0043] Provided herein, in another aspect, is a compound described herein, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, for use in treating a disease mediated by fibroblast activation protein. In some embodiments, the the disease mediated by fibroblast activation protein is selected from the group consisting of lipid and lipoprotein disorders, conditions and diseases which result from chronic fatty and fibrotic degeneration of organs due to accumulated lipid and specifically triglyceride accumulation and subsequent activation of profibrotic pathways, Type I or Type II Diabetes and clinical complications of Type I and Type II Diabetes, chronic intrahepatic or some forms of extrahepatic cholestatic conditions, liver fibrosis, acute intraheptic cholestatic conditions, obstructive or chronic inflammatory disorders that arise out of improper bile composition, gastrointestinal conditions with a reduced uptake of dietary fat and fat- soluble dietary vitamins, inflammatory bowel diseases, obesity and metabolic syndrome (combined conditions of dyslipidemia, diabetes and abnormally high body-mass index), persistent infections by intracellular bacteria or parasitic protozoae, non-malignant hyperproliferative disorders, malignant hyperproliferative disorders, colon adenocarcinoma and hepatocellular carcinoma in particular, liver steatosis and associated syndromes, Hepatitis B infection, Hepatitis C infection and/or of cholestatic and fibrotic effects that are associated with alcohol-induced cirrhosis or with viral-borne forms of hepatitis, liver failure or liver malfunction as an outcome of chronic liver diseases or of surgical liver resection, acute myocardial infarction, acute stroke, thrombosis which occurs as an endpoint of chronic obstructive atherosclerosis, osteoarthritis, rheumatoid arthritis, psoriasis, and cerebral infarction, individually or any combination thereof.

[0044] Provided herein, in another aspect, is a method of treating cancer in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of a compound described herein, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, or a therapeutically effective amount of a pharmaceutical composition described herein.

[0045] Provided herein, in another aspect, is a compound described herein, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, for use in treating cancer.

[0046] Provided herein, in another aspect, is a method of inhibiting degradation of fibroblast growth factor-21 in a subject, the method comprising administering, to the subject, a therapeutically effective amount of the compound, or its stereoisomer or mixture of stereoisomers, or its pharmaceutically acceptable salt described herein.

[0047] Provided herein, in another aspect, is a compound described herein, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, for use in inhibiting degradation of fibroblast growth factor-21.

[0048] Provided herein, in another aspect, is a method of preparing a compound of Formula la, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, the method comprising reacting a compound of Formula 2a with a compound of Formula 2aa under metal -mediated conditions to obtain a compound of Formula 3a:

R ₄ X (Formula 2aa); removing Boc from the compound of Formula 3a under reaction conditions to obtain the compound of Formula 4a: reacting the compound of Formula 4a with a compound of Formula 5 to obtain the compound of Formula la, or the isomer thereof, or the pharmaceutically acceptable salt thereof: wherein n is 1 or 2, wherein X is halogen, wherein R ₁ is a substituent selected from the group consisting of compounds of Formulas I-

V, wherein R ₂ is hydrogen or a C _1-3 alkyl, wherein R ₄ is a phenyl substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted with 1 to 3 halogens or amino, C _2-6 alkenyl, C _2-6 alkynyl, C _1-5 alkoxy, C _{1- 5} alkylthio, mono- or di-C _1-5 alkylamino, piperazinyl optionally substituted with acetyl, C _{1- 5} alkylsulfonylamino, C _1-5 alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _{1- 5} alkylaminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2-oxo- 1 ,2,3,4-tetrahydroquinolinyl; a naphthyl optionally substituted with halogen; or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2-c]pyridinyl, thieno[2,3- d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, acridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy, C _1-5 alkoxycarbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₁ C(O)R ₇ , and -C(O)NR ₆ R ₇ ; wherein R ₅ is hydrogen or a C _1-3 alkyl; wherein R ₆ is hydrogen or a C _1-3 alkyl group; and wherein R ₇ is a C _1-3 alkyl group optionally substituted with phenyl; a phenyl; or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0049] Provided herein, in another aspect, is a method of preparing a compound of Formula la, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, the method comprising reacting a compound of Formula 2a with a compound of Formula 2aa under metal -mediated conditions to obtain a compound of Formula 3a: R ₄ X (Formula 2aa); removing Boc from the compound of Formula 3a under reaction conditions to obtain the compound of Formula 4a: reacting the compound of Formula 4a with a compound of Formula 5 to obtain the compound of Formula la, or the isomer thereof, or the pharmaceutically acceptable salt thereof: wherein n is 1 or 2, wherein X is halogen, wherein R ₁ is a substituent selected from the group consisting of compounds of Formulas I-

V, wherein R ₂ is hydrogen or a C _1-3 alkyl, wherein R ₄ is a phenyl substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted with 1 to 3 halogens or amino, C _2-6 alkenyl, C _2-6 alkynyl, C _1-5 alkoxy, C _{1- 5} alkylthio, mono- or di-C _1-5 alkylamino, piperazinyl optionally substituted with acetyl, C _{1- 5} alkylsulfonylamino, C _1-5 alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _{1- 5} alkylaminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinobnyl, and 2-oxo- 1 ,2,3,4-tetrahydroquinolinyl; a naphthyl optionally substituted with halogen; or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinobnyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2-c]pyridinyl, thieno[2,3- d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, and acridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _1-5 alkoxy, C _1-5 alkoxycarbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, and C _1-5 alkylsulfonyl, and wherein R ₅ is hydrogen or a C _1-3 alkyl.

[0050] In some embodiments, the metal-mediated conditions comprise a palladium catalyst, a ligand, and base at a reaction temperature ranging from 50°C to 150°C.

[0051] Provided herein, in another aspect, is a method of preparing a compound of Formula 1b, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, the method comprising reacting a compound of Formula 2b with a compound of Formula 2ba under Mitsunobu reaction conditions to obtain a compound of Formula 3b:

removing Boc from the compound of Formula 3b under reaction conditions to obtain the compound of Formula 4b: reacting the compound of Formula 4b with a compound of Formula 5 to obtain the compound of Formula 1b, or the isomer thereof, or the pharmaceutically acceptable salt thereof: wherein n is 1 or 2, wherein R ₁ is a substituent selected from the group consisting of compounds of Formulas I-

V, wherein R ₂ is hydrogen or a C _1-3 alkyl, and wherein R ₄ is a phenyl substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted with 1 to 3 halogens or amino, C _2-6 alkenyl, C _2-6 alkynyl, C _1-5 alkoxy, C _{1- 5} alkylthio, mono- or di-C _1-5 alkylamino, piperazinyl optionally substituted with acetyl, C _{1- 5} alkylsulfonylamino, C _1-5 alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _{1- 5} alkylaminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinobnyl, and 2-oxo- 1 ,2,3,4-tetrahydroquinolinyl; a naphthyl optionally substituted with halogen; or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinobnyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2-c]pyridinyl, thieno[2,3- d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, acridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy, C _1-5 alkoxycarbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NRr,C(O)R ₇ . and -C(O)NR ₆ R ₇ : wherein R ₆ is hydrogen or a C _1-3 alkyl group; and wherein R ₇ is a C _1-3 alkyl group optionally substituted with phenyl; a phenyl; or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0052] Provided herein, in another aspect, is a method of preparing a compound of Formula 1b, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, the method comprising reacting a compound of Formula 2b with a compound of Formula 2ba under Mitsunobu reaction conditions to obtain a compound of Formula 3b: R ₄ -OH (Formula 2ba); removing Boc from the compound of Formula 3b under reaction conditions to obtain the compound of Formula 4b: reacting the compound of Formula 4b with a compound of Formula 5 to obtain the compound of Formula 1b, or the isomer thereof, or the pharmaceutically acceptable salt thereof: wherein n is 1 or 2, wherein R ₁ is a substituent selected from the group consisting of compounds of Formulas I-

V, wherein R ₂ is hydrogen or a C _1-3 alkyl, and wherein R ₄ is a phenyl substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted with 1 to 3 halogens or amino, C _2-6 alkenyl, C _2-6 alkynyl, C _1-5 alkoxy, C _{1- 5} alkylthio, mono- or di-C _1-5 alkylamino, piperazinyl optionally substituted with acetyl, C _{1- 5} alkylsulfonylamino, C _1-5 alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _{1- 5} alkylaminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2-oxo- 1 ,2,3,4-tetrahydroquinolinyl; a naphthyl optionally substituted with halogen; or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2-c]pyridinyl, thieno[2,3- d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, and acridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _1-5 alkoxy, C _1-5 alkoxycarbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, and C _1-5 alkylsulfonyl.

[0053] Provided herein, in another aspect, is a method of preparing a compound of Formula 1c, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, the method comprising reacting a compound of Formula 2c with a compound of Formula 2ca under amide coupling conditions to obtain a compound of Formula 3c: removing Boc from the compound of Formula 3c under reaction conditions to obtain the compound of Formula 4c: reacting the compound of Formula 4c with a compound of Formula 5 to obtain the compound of Formula 1c, or the isomer thereof, or the pharmaceutically acceptable salt thereof: wherein n is 1 or 2, wherein R ₁ is a substituent selected from the group consisting of compounds of Formulas I-

V, wherein R ₂ is hydrogen or a C _1-3 alkyl, and wherein R ₄ is a phenyl substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted with 1 to 3 halogens or amino, C _2-6 alkenyl, C _2-6 alkynyl, C _1-5 alkoxy, C _{1- 5} alkylthio, mono- or di-C _1-5 alkylamino, piperazinyl optionally substituted with acetyl, C _{1- 5} alkylsulfonylamino, C _1-5 alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _{1- 5} alkylaminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2-oxo- 1 ,2,3,4-tetrahydroquinolinyl; a naphthyl optionally substituted with halogen; or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2-c]pyridinyl, thieno[2,3- d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, acridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy, C _1-5 alkoxycarbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NRr,C(O)R ₇ . and -C(O)NR ₆ R ₇ : wherein R ₆ , is hydrogen or a Cwalkyl group; and wherein R ₇ is a C _1-3 alkyl group optionally substituted with phenyl; a phenyl; or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0054] Provided herein, in another aspect, is a method of preparing a compound of Formula 1c, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, the method comprising reacting a compound of Formula 2c with a compound of Formula 2ca under amide coupling conditions to obtain a compound of Formula 3c: removing Boc from the compound of Formula 3c under reaction conditions to obtain the compound of Formula 4c: reacting the compound of Formula 4c with a compound of Formula 5 to obtain the compound of Formula 1c, or the isomer thereof, or the pharmaceutically acceptable salt thereof: wherein n is 1 or 2, wherein R ₁ is a substituent selected from the group consisting of compounds of Formulas I-

V, wherein R ₂ is hydrogen or a C _1-3 alkyl, and wherein R ₄ is a phenyl substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted with 1 to 3 halogens or amino, C _2-6 alkenyl, C _2-6 alkynyl, C _1-5 alkoxy, C _{1- 5} alkylthio, mono- or di-C _1-5 alkylamino, piperazinyl optionally substituted with acetyl, C _{1- 5} alkylsulfonylamino, C _1-5 alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _{1- 5} alkylaminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2-oxo- 1 ,2,3,4-tetrahydroquinolinyl; a naphthyl optionally substituted with halogen; or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2-c]pyridinyl, thieno[2,3- d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, and acridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy, C _1-5 alkoxycarbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, and C _1-5 alkylsulfonyl.

[0055] Provided herein, in another aspect, is a method of preparing a compound of Formula 1d, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, the method comprising reacting a compound of Formula 5 with a compound of Formula 9 to obtain a compound of Formula 10: removing Boc from the compound of Formula 10 under reaction conditions to obtain the compound of Formula 11: reacting the compound of Formula 11 with a compound of Formula 1 la to obtain the compound of Formula 1d, or the isomer thereof, or the pharmaceutically acceptable salt thereof: R ₄ -(CH ₂ ) _m COOH (Formula 1la); wherein n is 1 or 2, wherein R ₁ is a substituent selected from the group consisting of compounds of Formulas I-

V, wherein R ₂ is hydrogen or a C _1-3 alkyl, wherein m is 0 or 1, and wherein R ₄ is a phenyl substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted with 1 to 3 halogens or amino, C _2-6 alkenyl, C _2-6 alkynyl, C _1-5 alkoxy, C _{1- 5} alkylthio, mono- or di-C _1-5 alkylamino, piperazinyl optionally substituted with acetyl, C _{1- 5} alkylsulfonylamino, C _1-5 alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _{1- 5} alkylaminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2-oxo- 1 ,2,3,4-tetrahydroquinolinyl; a naphthyl optionally substituted with halogen; or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2-c]pyridinyl, thieno[2,3- d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, acridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy, C _1-5 alkoxycarbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₆ C(O)R ₇ , and -C(O)NR ₆ R ₇ ; wherein R ₅ is hydrogen or a C _1-3 alkyl group; and wherein R ₇ is a C _1-3 alkyl group optionally substituted with phenyl; a phenyl; or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0056] Provided herein, in another aspect, is a method of preparing a compound of Formula 1d, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, the method comprising reacting a compound of Formula 5 with a compound of Formula 9 to obtain a compound of Formula 10: removing Boc from the compound of Formula 10 under reaction conditions to obtain the compound of Formula 11: reacting the compound of Formula 11 with a compound of Formula 1 la to obtain the compound of Formula 1d, or the isomer thereof, or the pharmaceutically acceptable salt thereof: R ₄ -(CH ₂ )mCOOH (Formula 1la); wherein n is 1 or 2, wherein R ₁ is a substituent selected from the group consisting of compounds of Formulas I-

V, wherein R ₂ is hydrogen or a C _1-3 alkyl, wherein m is 0 or 1, and wherein R ₄ is a phenyl substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted with 1 to 3 halogens or amino, C _2-6 alkenyl, C _2-6 alkynyl, C _1-5 alkoxy, C _{1- 5} alkylthio, mono- or di-C _1-5 alkylamino, piperazinyl optionally substituted with acetyl, C _{1- 5} alkylsulfonylamino, C _1-5 alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _{1- 5} alkylaminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2-oxo- 1 ,2,3,4-tetrahydroquinolinyl; a naphthyl optionally substituted with halogen; or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2-c]pyridinyl, thieno[2,3- d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, and acridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy, C _1-5 alkoxycarbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, and C _1-5 alkylsulfonyl.

[0057] Provided herein, in another aspect, is a method of preparing a compound of Formula le, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, the method comprising reacting a compound of Formula 5 with a compound of Formula 9 to obtain a compound of

Formula 10: removing Boc from the compound of Formula 10 under reaction conditions to obtain the compound of Formula 11: reacting the compound of Formula 11 with a compound of Formula 11b to obtain the compound of Formula 1d, or the isomer thereof, or the pharmaceutically acceptable salt thereof:

R ₄ -S(O) ₂ CI (Formula 11b); wherein n is 1 or 2, wherein R ₁ is a substituent selected from the group consisting of compounds of Formulas I-

V, wherein R ₂ is hydrogen or a C _1-3 alkyl, and wherein R ₄ is a phenyl substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted with 1 to 3 halogens or amino, C _2-6 alkenyl, C _2-6 alkynyl, C _1-5 alkoxy, C _{1- 5} alkylthio, mono- or di-C _1-5 alkylamino, piperazinyl optionally substituted with acetyl, C _{1- 5} alkylsulfonylamino, C _1-5 alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _{1- 5} alkylaminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2-oxo- 1 ,2,3,4-tetrahydroquinolinyl; a naphthyl optionally substituted with halogen; or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2-c]pyridinyl, thieno[2,3- d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, acridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy, C _1-5 alkoxycarbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₆ C(O)R ₇ , and -C(O)NR ₆ R ₇ ; wherein R ₅ is hydrogen or a Cwalkyl group; and wherein R ₇ is a C _1-3 alkyl group optionally substituted with phenyl; a phenyl; or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0058] Provided herein, in another aspect, is a method of preparing a compound of Formula le, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, the method comprising reacting a compound of Formula 5 with a compound of Formula 9 to obtain a compound of

Formula 10: removing Boc from the compound of Formula 10 under reaction conditions to obtain the compound of Formula 11: reacting the compound of Formula 11 with a compound of Formula 11b to obtain the compound of Formula 1d, or the isomer thereof, or the pharmaceutically acceptable salt thereof:

R ₄ -S(O) ₂ CI (Formula 11b); wherein n is 1 or 2, wherein R ₁ is a substituent selected from the group consisting of compounds of Formulas I-

V, wherein R ₂ is hydrogen or a C _1-3 alkyl, and wherein R ₄ is a phenyl substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted with 1 to 3 halogens or amino, C _2-6 alkenyl, C _2-6 alkynyl, C _1-5 alkoxy, C _{1- 5} alkylthio, mono- or di-C _1-5 alkylamino, piperazinyl optionally substituted with acetyl, C _{1- 5} alkylsulfonylamino, C _1-5 alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _{1- 5} alkylaminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2-oxo- 1 ,2,3,4-tetrahydroquinolinyl; a naphthyl optionally substituted with halogen; or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2-c]pyridinyl, thieno[2,3- d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, and acridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _1-5 alkoxy, C _1-5 alkoxycarbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, and C _1-5 alkylsulfonyl.

[0059] In some embodiments, the compound of Formula 5 is prepared by reacting a compound of Formula 6 with a compound of any one of Formulas R ₁ a-R ₁ e to obtain the compound of Formula 5:

[0060] Provided herein, in another aspect, is a method of preparing a compound of Formula 1b, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, the method comprising reacting a compound of Formula 5 with a compound of Formula 7 to obtain a compound of

Formula 8: reacting the compound of Formula 8 with a compound of Formula 2ba under Mitsunobu reaction conditions to obtain the compound of Formula 1b, or the isomer thereof, or the pharmaceutically acceptable salt thereof: R ₄ -OH (Formula 2ba); wherein n is 1 or 2, wherein R ₁ is a substituent selected from the group consisting of compounds of Formulas I-

V, wherein R ₂ is hydrogen or a C _1-3 alkyl, wherein R ₄ is a phenyl substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted with 1 to 3 halogens or amino, C _2-6 alkenyl, C _2-6 alkynyl, C _1-5 alkoxy, C _{1- 5} alkylthio, mono- or di-C _1-5 alkylamino, piperazinyl optionally substituted with acetyl, C _{1- 5} alkylsulfonylamino, C _1-5 alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _{1- 5} alkylaminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2-oxo- 1 ,2,3,4-tetrahydroquinolinyl; a naphthyl optionally substituted with halogen; or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2-c]pyridinyl, thieno[2,3- d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, acridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy, C _1-5 alkoxycarbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₆ C(O)R ₇ , and -C(O)NR ₆ R ₇ ; wherein R ₅ is hydrogen or a Cwalkyl group; and wherein R ₇ is a C _1-3 alkyl group optionally substituted with phenyl; a phenyl; or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0061] Provided herein, in another aspect, is a method of preparing a compound of Formula 1b, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, the method comprising reacting a compound of Formula 5 with a compound of Formula 7 to obtain a compound of Formula 8: reacting the compound of Formula 8 with a compound of Formula 2ba under Mitsunobu reaction conditions to obtain the compound of Formula 1b, or the isomer thereof, or the pharmaceutically acceptable salt thereof:

R ₄ -OH (Formula 2ba); wherein n is 1 or 2, wherein R ₁ is a substituent selected from the group consisting of compounds of Formulas I-

V, wherein R ₂ is hydrogen or a C _1-3 alkyl, wherein R ₄ is a phenyl substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted with 1 to 3 halogens or amino, C _2-6 alkenyl, C _2-6 alkynyl, C _1-5 alkoxy, C _{1- 5} alkylthio, mono- or di-C _1-5 alkylamino, piperazinyl optionally substituted with acetyl, C _{1- 5} alkylsulfonylamino, C _1-5 alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _{1- 5} alkylaminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2-oxo- 1 ,2,3,4-tetrahydroquinolinyl; a naphthyl optionally substituted with halogen; or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2-c]pyridinyl, thieno[2,3- d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, and acridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _1-5 alkoxy, C _1-5 alkoxycarbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, and C _1-5 alkylsulfonyl.

DETAILED DESCRIPTIONS

[0062] It is noted that, as used herein and in the appended claims, the singular forms "a", "an", and "the" include plural referents unless the context clearly dictates otherwise. It is further noted that the claims may be drafted to exclude any optional element. As such, this statement is intended to serve as antecedent basis for use of such exclusive terminology as "solely", "only" and the like in connection with the recitation of claim elements, or use of a "negative" limitation.

[0063] As used herein, the term "comprising" or "comprises" is intended to mean that the compositions and methods include the recited elements, but not excluding others. A composition or method "consisting essentially" of the elements as defined herein would not exclude other materials or steps that do not materially affect the basic and novel characteristic(s) of the claimed technology. "Consisting of' shall mean excluding more than trace elements of other ingredients and substantial method steps. Embodiments defined by each of these transition terms are within the scope of this technology. When an embodiment is defined by one of these terms (e.g., "comprising") it should be understood that this disclosure also includes alternative embodiments, such as "consisting essentially of' and "consisting of' for said embodiment.

[0064] "Substantially" or "essentially" means nearly totally or completely, for instance, 95%,

96%, 97%, 98%, 99%, or greater of some given quantity.

[0065] As used herein, the term "about" will be understood by persons of ordinary skill in the art and will vary to some extent depending upon the context in which it is used. If there are uses of the term which are not clear to persons of ordinary skill in the art given the context in which it is used, "about" will mean up to plus or minus 10% of the particular term.

[0066] Certain ranges are presented herein with numerical values being preceded by the term "about". The term "about" is used herein to provide literal support for the exact number that it precedes, as well as a number that is near to or approximately the number that the term precedes. In determining whether a number is near to or approximately a specifically recited number, the near or approximating unrecited number may be a number which, in the context in which it is presented, provides the substantial equivalent of the specifically recited number.

[0067] Where a range of values is provided, it is understood that each intervening value, to the tenth of the unit of the lower limit unless the context clearly dictates otherwise, between the upper and lower limit of that range and any other stated or intervening value in that stated range, is encompassed within the present technology. The upper and lower limits of these smaller ranges may independently be included in the smaller ranges and are also encompassed within the present technology, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the present technology.

[0068] In general, "substituted" refers to an organic group (e.g., an alkyl group) in which one or more bonds to a hydrogen atom contained therein are replaced by a bond to non-hydrogen or non- carbon atoms. Substituted groups also include groups in which one or more bonds to a carbon(s) or hydrogen(s) atom are replaced by one or more bonds, including double or triple bonds, to a heteroatom. The present disclosure is understood to include embodiments where, for instance a "substituted alkyl" optionally contains one or more alkene and/or alkyne. A substituted group will be substituted with one or more substituents, unless otherwise specified. In some embodiments, a substituted group is substituted with 1, 2, 3, 4, 5, or 6 substituents. Examples of substituent groups include: alkyls, haloalkyls, halogens (i.e., F, Cl, Br, and I); hydroxyls; alkoxy, alkenoxy, alkynoxy, aryloxy, aralkyloxy, heterocyclyloxy, and heterocyclylalkoxy groups; aryl groups; heteroaryl groups; cycloalkyl groups; heterocyclyl groups; carbonyls (oxo); carboxyls; esters; carbamates; urethanes; ureas; oximes; hydroxylamines; alkoxyamines; aralkoxyamines; thiols; sulfides; sulfoxides; sulfones; sulfonyls; sulfonamides; amines; N-oxides; hydrazines; hydrazides; hydrazones; azides; amides; ureas; amidines; guanidines; enamines; imides; isocyanates; isothiocyanates; cyanates; thiocyanates; imines; nitro groups; nitriles (i.e., CN); and the like. As used herein, an “optionally substituted” group refers to a group that is substituted or unsubstituted. As such, “optionally substituted” and “substituted or unsubstituted” may be used interchangeably.

[0069] Substituted ring groups such as substituted carbocyclic, substituted cycloalkyl, substituted aryl, substituted heterocyclic and substituted heteroaryl groups also include rings and fused ring systems in which a bond to a hydrogen atom is replaced with a bond to a carbon atom. Therefore, substituted carbocyclic, substituted cycloalkyl, substituted aryl, substituted heterocyclic and substituted heteroaryl groups may also be substituted with substituted or unsubstituted alkyl, alkenyl, and alkynyl groups as defined below.

[0070] As used herein, the term "carbocyclic group" or “carbocycle” includes aromatic (also referred to as aryl) and non-aromatic ring compounds (such as cycloalkyl) containing 3 or more ring members and that do not contain any heteroatom ring members. In some embodiments, a carbocycle includes 3 to 20 ring members, whereas other such groups have 3 to 6, 3 to 10, 3 to 12, 3 to 15, or 5 to 12 ring members.

[0071] As used herein, an "aryl group" refers to a cyclic aromatic hydrocarbon that does not contain heteroatoms. Aryl groups include monocyclic, bicyclic and polycyclic ring systems. Thus, aryl groups include, but are not limited to, phenyl, azulenyl, heptalenyl, biphenylenyl, indacenyl, fluorenyl, phenanthrenyl, triphenylenyl, pyrenyl, naphthacenyl, chrysenyl, biphenyl, anthracenyl, indenyl, indanyl, pentalenyl, and naphthyl groups. In some embodiments, aryl groups contain 6-14 carbons, and in others from 6 to 12 or even 6-10 carbon atoms in the ring portions of the groups. Although the phrase "aryl groups" includes groups containing fused rings, such as fused aromatic- aliphatic ring systems (e.g., indanyl, tetrahydronaphthyl, and the like), it does not include aryl groups that have other groups, such as alkyl or halo groups, bonded to one of the ring members. Rather, groups such as tolyl are referred to as substituted aryl groups. Representative substituted aryl groups may be mono-substituted or substituted more than once. For example, monosubstituted aryl groups include, but are not limited to, 2-, 3-, 4-, 5-, or 6-substituted phenyl or naphthyl groups, which may be substituted with substituents such as those listed above. [0072] As used herein, the term "cycloalkyl group" refers to a cyclic alkyl group such as, but not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl groups. In some embodiments, the cycloalkyl group has 3 to 8 carbon ring members, whereas in other embodiments the number of ring carbon atoms range from 3 to 5, 3 to 6, or 3 to 7. Cycloalkyl groups further include mono-, bicyclic and polycyclic ring systems, such as, for example bridged cycloalkyl groups as described below, and fused rings, such as, but not limited to, decalinyl, and the like. In some embodiments, polycyclic cycloalkyl groups have three rings. Substituted cycloalkyl groups may be substituted one or more times with non-hydrogen and non-carbon groups as defined above. However, substituted cycloalkyl groups also include rings that are substituted with straight or branched chain alkyl groups as defined above. Representative substituted cycloalkyl groups may be mono-substituted or substituted more than once, such as, but not limited to, 2,2-, 2,3-, 2,4- 2,5- or 2,6-di-substituted cyclohexyl groups, which may be substituted with substituents such as those listed above. In some embodiments, a cycloalkyl group has one or more alkene bonds, but is not aromatic.

[0073] As used herein, the term "heterocyclic group" or “heterocycle” includes aromatic (also referred to as heteroaryl) and non-aromatic ring compounds containing 3 or more ring members, of which one or more is a heteroatom such as, but not limited to, N, O, S or B. In some embodiments, heterocyclic groups include 3 to 20 ring members, whereas other such groups have 3 to 6, 3 to 10, 3 to 12, 3 to 15, or 5 to 12 ring members. Heterocyclic groups encompass unsaturated, partially saturated and saturated ring systems, such as, for example, imidazolyl, imidazolinyl and imidazolidinyl groups. The phrase "heterocyclic group" includes fused ring species including those comprising fused aromatic and non-aromatic groups, such as, for example, benzotriazolyl, 2,3- dihydrobenzo[1,4]dioxinyl, and benzo[1,3]dioxolyl. The phrase also includes bridged polycyclic ring systems containing a heteroatom such as, but not limited to, quinuclidyl. However, the phrase does not include heterocyclic groups that have other groups, such as alkyl, oxo or halo groups, bonded to one of the ring members. Rather, these are referred to as "substituted heterocyclic groups". Heterocyclic groups include, but are not limited to, acridinyl, aziridinyl, azetidinyl, pyrrolidinyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl, tetrahydrothiophenyl, tetrahydrofuranyl, dioxolyl, furanyl, thiophenyl, pyrrolyl, pyrrolinyl, imidazolyl, imidazolinyl, pyrazolyl, pyrazolinyl, triazolyl, tetrazolyl, oxazolyl, isoxazolyl, thiazolyl, thiazolinyl, isothiazolyl, thiadiazolyl, oxadiazolyl, chromenonyl, piperidyl, piperazinyl, morpholinyl, thiomorpholinyl, tetrahydropyranyl, tetrahydrothiopyranyl, oxathiane, dioxyl, dithianyl, pyranyl, pyridyl, pyrimidinyl, pyridazinyl, pyrazinyl, triazinyl, dihydropyridyl, dihydrodithiinyl, dihydrodithionyl, homopiperazinyl, quinuclidyl, indolyl, indolinyl, isoindolyl,azaindolyl (pyrrolopyridyl), indazolyl, indolizinyl, benzotriazolyl, benzimidazolyl, benzofuranyl, benzothiophenyl, benzthiazolyl, benzoxadiazolyl, benzoxazinyl, benzodithiinyl, benzoxathiinyl, benzothiazinyl, benzoxazolyl, benzothiazolyl, benzothiadiazolyl, benzo[1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2- dihydroquinolinyl, 2-oxo-1,2,3,4-tetrahydroquinolinyl, pyrazolopyridyl, imidazopyridyl (azabenzimidazolyl), triazolopyridyl, isoxazolopyridyl, furo[3,2-c]pyridinyl, thieno[2,3- d]pyrimidinyl, thieno[3,2-c]pyridinyl, purinyl, xanthinyl, adeninyl, guaninyl, quinolinyl, isoquinolinyl, quinolizinyl, quinoxalinyl, quinazolinyl, cinnolinyl, phthalazinyl, naphthyridinyl, pteridinyl, thianaphthalenyl, dihydrobenzothiazinyl, dihydrobenzofuranyl, dihydroindolyl, dihydrobenzodioxinyl, tetrahydroindolyl, tetrahydroindazolyl, tetrahydrobenzimidazolyl, tetrahydrobenzotriazolyl, tetrahydropyrrolopyridyl, tetrahydropyrazolopyridyl, tetrahydroimidazopyridyl, tetrahydrotriazolopyridyl, and tetrahydroquinolinyl groups. Representative substituted heterocyclic groups may be mono-substituted or substituted more than once, such as, but not limited to, pyridyl or piperazinyl groups, which are 2-, 3-, 4-, 5-, or 6-substituted, or disubstituted with various substituents such as those listed above. In some embodiments, the term “naphthyridinyl” refers to 1,8 -naphthyridinyl; 2,6-naphthyridinyl; or 1,5-naphthyridinyl, or any combination of two or more thereof.

[0074] As used herein, the term "heteroaryl group" refers to an aromatic ring compound containing 5 or more ring members, of which, one or more is a heteroatom such as, but not limited to, N, O, S or B. In some embodiments, one or more heteroatoms are independently chosen from N, O, or S. In some embodiments, 1 to 4 heteroatoms are independently chosen from N, O, or S. In some embodiments, 1 to 5 heteroatoms are independently chosen from N, O, or S. In some embodiments, heteroaryl groups include 5 to 14 ring members, whereas other such groups have 5 to 6, 5 to 9, 5 to 10, 6 to 9, 6 to 10, or 6 to 14 ring members. For example, a 5-membered heteroaryl group has 5 ring members; a 6-membered heteroaryl group has 6 ring members; and a 9-membered heteroaryl group has 9 ring members (such as, but not limited to, benzothiophene). Heteroaryl groups include, but are not limited to, groups such as pyrrolyl, pyrazolyl, triazolyl, tetrazolyl, oxazolyl, isoxazolyl, thiazolyl, pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, thiophenyl, benzothiophenyl, furanyl, benzofuranyl, indolyl, azaindolyl (pyrrolopyridyl), indazolyl, benzimidazolyl, imidazopyridyl (azabenzimidazolyl), pyrazolopyridyl, triazolopyridyl, benzotriazolyl, benzoxazolyl, benzothiazolyl, benzothiadiazolyl, imidazopyridyl, isoxazolopyridyl, thianaphthalenyl, purinyl, xanthinyl, adeninyl, guaninyl, naphthyridinyl, quinolinyl, isoquinolinyl, tetrahydroquinolinyl, quinoxalinyl, and quinazolinyl groups. Although the phrase "heteroaryl groups" includes fused ring compounds such as indolyl and 2,3- dihydro indolyl, the phrase does not include heteroaryl groups that have other groups bonded to one of the ring members, such as alkyl groups. Rather, heteroaryl groups with such substitution are referred to as "substituted heteroaryl groups." Representative substituted heteroaryl groups may be substituted one or more times with various substituents such as those listed above. An azolyl group is a 5- membered heteroaryl group containing a nitrogen atom and at least one other atom selected from nitrogen, sulfur, and oxygen as part of the ring. Azolyl groups include imidazole, pyrazole, 1,2,3- triazole, 1,2,4-triazole, tetrazole, pentazole, oxazole, isoxazole, 1,2,4-oxadiazole, 1,2,5-oxadiazole,

1.3.4-oxadiazole, thiazole, isothiazole, 1,2,3-thiadiazole, 1,2,4-thiadiazole, 1,2,5-thiadiazole, and

1.3.4-thiadiazole.

[0075] As used herein, the term "alkyl" refers to an aliphatic hydrocarbon radical, which encompasses both straight and branched hydrocarbon radicals. In some embodiments, alkyl has from 1 to about 20 carbon atoms, from 1 to 12 carbons, from 1 to 8 carbons, 1 to 6 carbons, or 1 to 4 carbon atoms. For example, C _1-6 alkyl refers to an aliphatic hydrocarbon having 1 to 6 carbons, which includes methyl, ethyl, propyl, n-butyl, n-pentyl, n-hexyl, isopropyl, isobutyl, sec-butyl, tert-butyl, neopentyl, isopentyl, and the like.

[0076] In addition, as used herein, the term "hydroxy" is defined as -OH.

[0077] As used herein, the term "alkoxy," unless particularly defined herein, refers to a radical formed by substituting the hydrogen atom of a hydroxyl group with an alkyl, as defined above. For example, C _1-6 alkoxy includes methoxy, ethoxy, propoxy, n-butoxy, n-pentyloxy, isopropoxy, sec- butoxy, tert-butoxy, neopentyloxy, isopentyloxy, and the like.

[0078] In addition, as used herein, the term "halogen" refers to fluorine, bromine, chlorine, and iodine.

[0079] In addition, as used herein, the term "amino" is defined as -NH2, and the term "alkylamino" refers to a mono- or di-alkyl substituted amino. For example, C _1-6 alkylamino includes mono- or di-C _1-6 alkyl substituted amino.

[0080] In addition, as used herein, the term "alkylthio" is defined as -SR* (wherein R* is alkyl), and the term "cyano" is defined as -CN.

[0081] Those of skill in the art will appreciate that compounds of the present technology may exhibit the phenomena of tautomerism, conformational isomerism, geometric isomerism and/or optical isomerism. As the formula drawings within the specification and claims can represent only one of the possible tautomeric, conformational isomeric, optical isomeric or geometric isomeric forms, it should be understood that the present technology encompasses any tautomeric, conformational isomeric, optical isomeric and/or geometric isomeric forms of the compounds having one or more of the utilities described herein, as well as mixtures of these various different forms. As used herein, "isomer" refers to a tautomer, conformation isomer, optical isomer, geometric isomer, or any combination thereof, of a compound.

[0082] As readily understood by one skilled in the art, a wide variety of functional groups and other structures may exhibit tautomerism, and all tautomers of compounds as described herein are within the scope of the present technology.

[0083] Stereoisomers of compounds, also known as "optical isomers," include all chiral, diastereomeric, and racemic forms of a structure, unless the specific stereochemistry is expressly indicated. Thus, compounds used in the present technology include enriched or resolved optical isomers at any or all stereogenic atoms as are apparent from the depictions. Both racemic and diastereomeric mixtures, as well as the individual optical isomers can be isolated or synthesized so as to be substantially free of their enantiomeric or diastereomeric partners, and these are all within the scope of the present technology.

[0084] By "pharmaceutically acceptable" is meant a material that is not biologically or otherwise undesirable, e.g., the material may be incorporated into a pharmaceutical composition administered to a patient without causing any undesirable biological effects or interacting in a deleterious manner with any of the other components of the composition in which it is contained. When the term "pharmaceutically acceptable" is used to refer to a pharmaceutical carrier or excipient, it is implied that the carrier or excipient has met the required standards of toxicological and manufacturing testing or that it is included on the Inactive Ingredient Guide prepared by the U.S. Food and Drug administration.

[0085] Generally, reference to a certain moiety capable of being protected (such as hydroxy, amine, carbonyl, etc.) includes the protected groups in some embodiments of the disclosure. For example, in some embodiments, an -OH moiety as included herein also includes -OP, where P is a protecting group. Protecting groups, as referred to herein may be selected by one of ordinary skill in the art, and include the groups and strategies set forth in the art, for example, those such as described in R. Larock, Comprehensive Organic Transformations, VCH Publishers (1989); T. W. Greene and P. G. M. Wuts, Greene's protective groups in organic synthesis, John Wiley & Sons (2006); L. Fieser and M. Fieser, Fieser and Fieser's Reagents for Organic Synthesis, John Wiley and Sons (1994); and L. Paquette, ed., Encyclopedia of Reagents for Organic Synthesis, John Wiley and Sons (1995), and subsequent editions thereof.

[0086] "Subject" refers to an animal, such as a mammal (including a human), that has been or will be the object of treatment, observation or experiment. "Subject" and "patient" may be used interchangeably, unless otherwise indicated. The methods described herein may be useful in human therapy and/or veterinary applications. In some embodiments, the subject is a mammal. In some embodiments, the subject is a human.

[0087] The terms "therapeutically effective amount" and "effective amount" are used interchangibly and refer to an amount of a compound that is sufficient to effect treatment as defined below, when administered to a patient (e.g., a human) in need of such treatment in one or more doses. The therapeutically effective amount will vary depending upon the patient, the disease being treated, the weight and/or age of the patient, the severity of the disease, or the manner of administration as determined by a qualified prescriber or care giver.

[0088] The term "treatment" or "treating" means administering a compound disclosed herein for the purpose of: (i) delaying the onset of a disease, that is, causing the clinical symptoms of the disease not to develop or delaying the development thereof; (ii) inhibiting the disease, that is, arresting the development of clinical symptoms; and/or (iii) relieving the disease, that is, causing the regression of clinical symptoms or the severity thereof.

[0089] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this present technology belongs. Although any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of the present technology, representative illustrative methods and materials are described herein.

[0090] The present inventors found that specific pyrrolidine or piperidine compounds, while exhibiting low inhibitory activity on other DPP family enzymes (DPP4, DPP7, DPP8, DPP9, PREP), exhibit selective inhibitory activity on FAP Therefore, the pyrrolidine or piperidine compounds or their salts can be usefully used in the treatment and prophylaxis of various FAP -mediated diseases, for example, cancer, wound healing, obesity, diabetes, steatosis, nonalcoholic steatohepatitis (NASH), and liver cirrhosis.

[0091] Therefore, the present technology provides the pyrrolidine and piperidine compounds, their stereoisomers, or their pharmaceutically acceptable salts, preparation processes thereof, pharmaceutical compositions comprising the same, and the use thereof.

[0092] In accordance with one aspect of the present technology, there is provided a pyrrolidine or piperidine compound, its stereoisomer or mixture of stereoisomers, or its pharmaceutically acceptable salt.

[0093] In accordance with another aspect of the present technology, there is provided a preparation process of the pyrrolidine or piperidine compound.

[0094] In accordance with another aspect of the present technology, there is provided a pharmaceutical composition comprising the pyrrolidine or piperidine compound as an active ingredient.

[0095] In accordance with another aspect of the present technology, there is provided a method of treatment comprising administering the pyrrolidine or piperidine compound described herein.

[0096] In accordance with another aspect of the present technology, there is provided the use of the pyrrolidine or piperidine compound or its stereoisomer or mixture of stereoisomers, or its pharmaceutically acceptable salt in the manufacture of a medicament for inhibition of fibroblast activation proteins.

[0097] It was found by the present technology that the compounds according to the present technology, i.e., the pyrrolidine or piperidine compounds, or their stereoisomers, or their pharmaceutically acceptable salts, exhibit inhibitory activity on FAR Therefore, the compounds according to the present technology, or stereoisomers thereof, or pharmaceutically acceptable salts thereof can be usefully applied for the treatment and prophylaxis of FAP-mediated various diseases, for example, cancer, wound healing, obesity, diabetes, steatosis, nonalcoholic steatohepatitis (NASH), and liver cirrhosis.

[0098] In one aspect, provided herein is a compound of Formula X, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof: wherein n is 1 or 2;

R ₁ is a substituent selected from the group consisting of compounds of Formulas I-V:

R ₂ is hydrogen or a C _1-3 alkyl;

R ₃ is a 5- to 12-membered substituted or unsubstituted heteroaryl, wherein said heteroaryl contains 1 to 3 heteroatoms independently selected from O, N, and S, a 3- to 12-membered substituted or unsubstituted non-aromatic heterocycle, wherein said heterocycle contains 1 to 3 heteroatoms independently selected from O, N, and S,

-NR ₄ R ₅ , -OR ₄ , -C(O)NHR ₄ , -NHC(O)(CH ₂ )mR ₄ , or -NHS(O) ₂ R ₄ , wherein m is 0 or 1; R ₄ is a substituted phenyl; a substituted or unsubstituted naphthyl; a substituted or unsubstituted non aromatic heterocycle; or a substituted or unsubstituted heteroaryl; and

R ₅ is hydrogen or a C _1-3 alkyl.

[0099] In another aspect, provided herein is a compound of Formula Xa, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof: wherein n is 1 or 2,

R ₁ is a substituent selected from the group consisting of compounds of Formulas I-V:

R ₂ is hydrogen or a C _1-3 alkyl;

R ₃ is a 5- to 12-membered substituted or unsubstituted heteroaryl, wherein said heteroaryl contains 1 to 3 heteroatoms independently selected from O, N, and S, a 3- to 12-membered substituted or unsubstituted non-aromatic heterocycle, wherein said heterocycle contains 1 to 3 heteroatoms independently selected from O, N, and S,

-NR ₄ R ₅ , -OR ₄ , -C(O)NHR ₄ , -NHC(O)(CH ₂ )mR ₄ , or -NHS(O) ₂ R ₄ , wherein m is 0 or 1; R ₄ is a substituted phenyl; a substituted or unsubstituted naphthyl; a substituted or unsubstituted non aromatic heterocycle; or a substituted or unsubstituted heteroaryl; and

R ₅ is hydrogen or a C _1-3 alkyl.

[0100] In another aspect, provided herein is a compound of Formula Xb, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof: wherein n is 1 or 2;

R ₁ is a substituent selected from the group consisting of compounds of Formulas I-V,

R ₂ is hydrogen or a C _1-3 alkyl;

R ₃ is a 5- to 12-membered substituted or unsubstituted heteroaryl, wherein said heteroaryl contains 1 to 3 heteroatoms independently selected from O, N, and S, a 3- to 12-membered substituted or unsubstituted non-aromatic heterocycle, wherein said heterocycle contains 1 to 3 heteroatoms independently selected from O, N, and S,

-NR ₄ R ₅ , -OR ₄ , -C(O)NHR ₄ , -NHC(O)(CH ₂ )mR ₄ , or -NHS(O) ₂ R ₄ , wherein m is 0 or 1 ;

R ₄ is a substituted phenyl; a substituted or unsubstituted naphthyl; a substituted or unsubstituted non aromatic heterocycle; or a substituted or unsubstituted heteroaryl; and

R ₅ is hydrogen or a C _1-3 alkyl.

[0101] In another aspect, provided herein is a compound of Formula la, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof: wherein R ₁ , R ₂ , R ₄ , R ₅ , and n are as defined in the embodiments above or below. [0102] In another aspect, provided herein is a compound of Formula 1b, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof: wherein R ₁ , R ₂ , R ₄ , and n are as defined in the embodiments above or below.

[0103] In another aspect, provided herein is a compound of Formula 1c, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof: wherein R ₁ , R ₂ , R ₄ , and n are as defined in the embodiments above or below.

[0104] In another aspect, provided herein is a compound of Formula 1d, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof: wherein R ₁ , R ₂ , R ₄ , m, and n are as defined in the embodiments above or below.

[0105] In another aspect, provided herein is a compound of Formula le, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof: wherein R ₁ , R ₂ , R ₄ , and n are as defined in the embodiments above or below.

[0106] In some embodiments of Formulas X, Xa, Xb, 1a, 1b, 1c, 1d, and le, R ₄ is a phenyl substituted with 1 to 3 Z; a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2- dihydroquinolinyl, and 2-oxo-1,2,3,4-tetrahydroquinolinyl, wherein said heterocycle is optionally substituted with 1 to 3 Z; a naphthyl optionally substituted with 1 to 3 Z, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2- c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, and acridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z; and each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted by 1 to 3 Z ¹ , C _2-6 alkenyl optionally substituted by Z ¹ , C _2-6 alkynyl optionally substituted by Z ¹ , C _1-5 alkoxy optionally substituted by 1 to 3 Z ¹ , C _1-5 alkylthio optionally substituted by Z ¹ , mono- or di-C _1-5 alkylamino optionally substituted by Z ¹ , piperazinyl optionally substituted by Z ¹ , C _1-5 alkylsulfonylamino optionally substituted by Z ¹ , C _1-5 alkylcarbonylamino optionally substituted by Z ¹ , aminosulfonyl optionally substituted by Z ¹ , aminocarbonyl optionally substituted by Z ¹ , C _{1- 5} alkylaminocarbonyl optionally substituted by Z ¹ , phenyl optionally substituted by Z ¹ , phenoxy optionally substituted by Z ¹ , benzyl optionally substituted by Z ¹ , benzoyl optionally substituted by Z ¹ , phenylaminocarbonyl optionally substituted by Z ¹ , pyrazolyl optionally substituted by Z ¹ , benzoxazolyl optionally substituted by Z ¹ , C 1-5 alkoxy carbonyl optionally substituted by Z ¹ , benzyloxy optionally substituted by Z ¹ , and C _1-5 alkylsulfonyl optionally substituted by Z ¹ ; wherein each Z ¹ is independently chosen from halogen, hydroxyl, amino, C _1-5 alkylamino, cyano, acetyl, C _1-5 alkyl, C _1-5 haloalkyl. C _3-6 carbocycle, C _3-6 heterocycle, wherein said C _3-6 carbocycle and said C _3-6 heterocycle are optionally substituted with halogen, hydroxyl, C _1-5 alkyl or C _1-5 haloalkyl.

[0107] In some embodiments of Formulas X, Xa, Xb, 1a, 1b, 1c, 1d, and le, R ₄ is a phenyl substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted with 1 to 3 halogens or amino, C _2-6 alkenyl, C _2-6 alkynyl, C _1-5 alkoxy, C _1-5 alkylthio, mono- or di-C _1-5 alkylamino, piperazinyl optionally substituted with acetyl, C _1-5 alkylsulfonylamino, C _1-5 alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _1-5 alkylaminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; or

R ₄ a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinobnyl, and 2-oxo- 1,2,3,4-tetrahydroquinolinyl; or R ₄ is a naphthyl optionally substituted with halogen; or R ₄ is a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2-c]pyridinyl, thieno[2,3- d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl and acridinyl, wherein the heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxycarbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, and C _1-5 alkylsulfonyl.

[0108] In another aspect, the present technology provides a compound having inhibitory activity on FAP or its salt, that is, a compound of Formula 1, or its stereoisomer or mixture of stereoisomers, or its pharmaceutically acceptable salt: wherein n is 1 or 2;

R ₁ is selected from the group consisting of Formulas I, II, III, IV, and IV;

R ₂ is a hydrogen or a C _1-3 alkyl;

R ₃ is a 5- to 12-membered heteroaryl, wherein said heteroaryl contains 1 to 3 heteroatoms independently selected from O, N, and S and is optionally substituted by 1 to 3 Z, a 3 - to 12-membered non-aromatic heterocycle, wherein said heterocycle contains 1 to 3 heteroatoms independently selected from O, N, and S and is optionally substituted by 1 to 3

Z,

-NR ₄ R ₅ , -OR ₄ , -C(O)NHR ₄ , -NHC(O)(CH ₂ )mR ₄ , -NHS(O) ₂ R ₄ , -(CH ₂ ) _m CH ₂ R ₄ , or - (CH ₂ ) _m NHR ₄ , wherein m is 0 or 1; R ₄ is a phenyl substituted with 1 to 3 Z, a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2- oxo-1,2,3,4-tetrahydroquinolinyl, wherein said heterocycle is optionally substituted with 1 to 3 Z, a naphthyl optionally substituted with 1 to 3 Z, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, fiiro[3,2- c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, acridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z;

R ₅ is a hydrogen or a C _1-3 alkyl; and each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted by 1 to 3 Z ¹ , C _2-6 alkenyl optionally substituted by Z ¹ , C _2-6 alkynyl optionally substituted by Z ¹ , C _1-5 alkoxy optionally substituted by 1 to 3 Z ¹ , C _1-5 alkylthio optionally substituted by Z ¹ , mono- or di-C _1-5 alkylamino optionally substituted by Z ¹ , piperazinyl optionally substituted by Z ¹ , C _1-5 alkylsulfonylamino optionally substituted by Z ¹ , C _1-5 alky1carbonylamino optionally substituted by Z ¹ , aminosulfonyl optionally substituted by Z ¹ , aminocarbonyl optionally substituted by Z ¹ , C _{1- 5} alkylaminocarbonyl optionally substituted by Z ¹ , phenyl optionally substituted by Z ¹ , phenoxy optionally substituted by Z ¹ , benzyl optionally substituted by Z ¹ , benzoyl optionally substituted by Z ¹ , phenylaminocarbonyl optionally substituted by Z ¹ , pyrazolyl optionally substituted by Z ¹ , benzoxazolyl optionally substituted by Z ¹ , C 1-5 alkoxy carbonyl optionally substituted by Z ¹ , benzyloxy optionally substituted by Z ¹ , C _1-5 alkylsulfonyl optionally substituted by Z ¹ , acetyl, morpholinyl optionally substituted by Z ¹ , -NR ₁ C(O)R ₇ , and - C(O)NR6R ₇ ; wherein each Z ¹ is independently chosen from halogen, hydroxyl, amino, C _1-5 alkylamino, cyano, acetyl, C _1-5 alkyl, C _1-5 haloalkyl. C _3-6 carbocycle, C _3-6 heterocycle, wherein said C _3-6 carbocycle and said C _3-6 heterocycle are optionally substituted with halogen, hydroxyl, C _{1- 5} alkyl, or C _1-5 haloalkyl;

R ₆ is hydrogen or a C _1-3 alkyl group;

R ₇ is a C _1-3 alkyl group optionally substituted with phenyl, a phenyl optionally substituted with 1 to 3 Z ² , or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2- c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, acridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z ² ; and each Z ² is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, hydroxycarbonyl, C _1-5 alky l optionally substituted by 1 to 3 Z ³ , C _2-6 alkenyl, C _2-6 alkynyl, C _{1- 5} alk oxy optionally substituted byl to 3 Z ³ , C _1-5 alky lthio. mono- or di-C _1-5 alkylamino, piperazinyl optionally substituted by Z ³ , C _1-5 alkylsulfonylamino, C _1-5 alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _1-5 alky laminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; wherein each Z ³ is independently chosen from halogen, amino, or acetyl.

[0109] In some embodiments of a compound of Formula 1, or its stereoisomer or mixture of stereoisomers, or its pharmaceutically acceptable salt: n is 1 or 2;

R ₁ is selected from the group consisting of Formulas I, II, III, IV, and IV;

R ₂ is a hydrogen or a C _1-3 alkyl;

R ₃ is a 5- to 12-membered heteroaryl, wherein said heteroaryl contains 1 to 3 heteroatoms independently selected from O, N, and S and is optionally substituted by 1 to 3 Z, a 3 - to 12-membered non-aromatic heterocycle, wherein said heterocycle contains 1 to 3 heteroatoms independently selected from O, N, and S and is optionally substituted by 1 to 3

Z,

-NR ₄ R ₅ , -OR ₄ , -C(O)NHR ₄ , -NHC(O)(CH ₂ )mR ₄ , or -NHS(O) ₂ R ₄ , wherein m is 0 or 1; R ₄ is a phenyl substituted with 1 to 3 Z, a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2- oxo-1,2,3,4-tetrahydroquinolinyl, wherein said heterocycle is optionally substituted with 1 to 3 Z, a naphthyl optionally substituted with 1 to 3 Z, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, fiiro[3,2- c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, and acridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z;

R ₅ is a hydrogen or a C _1-3 alkyl; and each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted by 1 to 3 Z ¹ , C _2-6 alkenyl optionally substituted by Z ¹ , C _2-6 alkynyl optionally substituted by Z ¹ , C _1-5 alkoxy optionally substituted by 1 to 3 Z ¹ , C _1-5 alkylthio optionally substituted by Z ¹ , mono- or di-C _1-5 alkylamino optionally substituted by Z ¹ , piperazinyl optionally substituted by Z ¹ , C _1-5 alkylsulfonylamino optionally substituted by Z ¹ , C _1-5 alky1carbonylamino optionally substituted by Z ¹ , aminosulfonyl optionally substituted by Z ¹ , aminocarbonyl optionally substituted by Z ¹ , C _{1- 5} alkylaminocarbonyl optionally substituted by Z ¹ , phenyl optionally substituted by Z ¹ , phenoxy optionally substituted by Z ¹ , benzyl optionally substituted by Z ¹ , benzoyl optionally substituted by Z ¹ , phenylaminocarbonyl optionally substituted by Z ¹ , pyrazolyl optionally substituted by Z ¹ , benzoxazolyl optionally substituted by Z ¹ , C 1-5 alkoxy carbonyl optionally substituted by Z ¹ , benzyloxy optionally substituted by Z ¹ , and C _1-5 alkylsulfonyl optionally substituted by Z ¹ ; wherein each Z ¹ is independently chosen from halogen, hydroxyl, amino, C _1-5 alkylamino, cyano, acetyl, C _1-5 alkyl, C _1-5 haloalkyl. C _3-6 carbocycle, C _3-6 heterocycle, wherein said C _3-6 carbocycle and said C _3-6 heterocycle are optionally substituted with halogen, hydroxyl, C _{1- 5} alkyl or C _1-5 haloalkyl.

[0110] In some embodiments, R ₁ is selected from the group consisting of compounds of Formulas I, II, III, and V:

In some embodiments, R ₁ is a substituent of Formula I. In some embodiments, R ₁ is a substituent of Formula II. In some embodiments, R ₁ is a substituent of Formula III. In some embodiments, R ₁ is a substituent of Formula IV. In some embodiments, R ₁ is a substituent of Formula V.

[0111] In some embodiments, R ₁ is a substituent selected from the group consisting of compounds of Formulas Ia-Va,

[0112] In some embodiments, R ₁ is a substituent selected from the group consisting of compounds of Formulas la, Ila, IlIa, and Va:

[0113] In some embodiments, R ₁ is a compound of Formula la: [0114] In some embodiments, R ₁ is a compound of Formula Ila:

[0115] In some embodiments, R ₁ is a compound of Formula Ilia:

[0116] In some embodiments, R ₁ is a compound of Formula IVa:

[0117] In some embodiments, R ₁ is a compound of Formula Va:

[0118] In some embodiments, R ₂ is hydrogen. In some embodiments, R ₂ is a C _1-3 alkyl.

[0119] In some embodiments, m is 0. In some embodiments, m is 1.

[0120] In some embodiments, n is 1. In some embodiments, n is 2.

[0121] In some embodiments, R ₃ is a substituent selected from the group consisting of -NR ₄ R ₅ , - OR ₄ , -C(O)NHRi, -NHC(O)(CH ₂ ) _m R ₄ , and -NHS(O) ₂ R ₄ . In some embodiments, R ₃ is a substituent selected from the group consisting of -NR ₄ R ₅ , -OR ₄ , and -NHC(O)(CH ₂ ) _m R ₄ . In some embodiments,

R ₃ is a substituent selected from the group consisting of -NR ₄ R ₅ and -OR ₄ . In some embodiments, R ₃ is -NR ₄ R ₅ . In some embodiments, R ₃ is -OR ₄ . In some embodiments, R ₃ is -C(O)NHR ₄ . In some embodiments, R ₃ is -NHC(O)(CH ₂ ) _m R ₄ . In some embodiments, R ₃ is -NHS(O) ₂ R ₄ .

[0122] In some embodiments, R ₃ is a 3- to 12-membered non-aromatic heterocycle containing 1-3 nitrogens, wherein said heterocycle is optionally substituted by 1 to 3 Z. In some embodiments, R ₃ is a 5- to 12-membered heteroaryl optionally substituted by 1 to 3 Z. In some embodiments, R ₃ is 2-oxo- 2,3 -dihydro- IH-benzo [d]imidazol- 1 -yl .

[0123] In some embodiments, R ₄ is a phenyl substituted with a substituent selected from the group consisting of piperazinyl optionally substituted with acetyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; or R ₄ is a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2- oxo-1,2,3,4-tetrahydroquinolinyl; or R ₄ is a naphthyl optionally substituted with halogen; or or R ₄ is a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, quinazolinyl, benzofuranyl, benzothiophenyl, furo[3,2-c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2- c]pyridinyl, isoxazolyl, and acridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, halogen, C _1-5 alky l optionally substituted 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1- 5alkoxycarbonyl, benzyloxy, and phenyl optionally substituted with halogen.

[0124] In some embodiments, R ₄ is a phenyl substituted with phenyl; or R ₄ is a naphthyl; or or R ₄ is a heteroaryl selected from the group consisting of quinolinyl, isoquinolinyl, benzofuranyl, benzothiophenyl, and furo[3,2-c]pyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, and C _1-5 alkoxy optionally substituted with 1 to 3 halogens.

[0125] In some embodiments, R ₄ is a phenyl substituted with phenyl; or R ₄ is a naphthyl; or or R ₄ is a heteroaryl selected from the group consisting of quinolinyl, isoquinolinyl, benzofuranyl, and benzothiophenyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, halogen, C _1-5 alkyl, and C _1-5 alkoxy.

[0126] In some embodiments, R ₄ is a phenyl substituted with a substituent selected from the group consisting of piperazinyl optionally substituted with acetyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; or R ₄ is a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2- oxo-1,2,3,4-tetrahydroquinolinyl; or R ₄ is a naphthyl optionally substituted with halogen; or or R ₄ is a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, quinazolinyl, thieno[2,3-d]pyrimidinyl, isoxazolyl, and acridinyl wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of halogen, C _{1- 5} alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, benzyloxy, and phenyl optionally substituted with halogen.

[0127] In some embodiments, R ₄ is a phenyl substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, carboxylic acid, C _1-5 alkyl optionally substituted with 1 to 3 halogens or amino, C _2-6 alkenyl, C _2-6 alkynyl, C _{1- 5} alkoxy, C _1-5 alkylthio, mono- or di-C _1-5 alkylamino, piperazinyl optionally substituted with acetyl, C _{1- 5} alkylsulfonylamino, C _1-5 alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _{1- 5} alkylaminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl.

[0128] In some embodiments, R ₄ is a phenyl substituted with a substituent selected from the group consisting of piperazinyl optionally substituted with acetyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl. In some embodiments, R ₄ is a phenyl substituted with phenyl.

[0129] In some embodiments, R ₄ is a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, dihydrobenzo[1,4]dioxinyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2- dihydroquinolinyl, and 2-oxo-1,2,3,4-tetrahydroquinolinyl. In some embodiments, R ₄ is a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, 2,3-dihydrobenzofuranyl, 2-oxo-1,2- dihydroquinolinyl, and 2-oxo-1,2,3,4-tetrahydroquinolinyl.

[0130] In some embodiments, R ₄ is a naphthyl optionally substituted with halogen. In some embodiments, R ₄ is a naphthyl.

[0131] In some embodiments, R ₄ is a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2-c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, acridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _{1- 5} alkylsulfonyl, morpholinyl, -NR ₁ C(O)R ₇ , and -C(O)NR ₆ R ₇ . In some embodiments, R ₄ is a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzofuranyl, indolinyl, benzimidazolyl, benzothiophenyl, benzothiazolyl, furo[3,2- c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, acridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1- 5alkoxycarbonyl, benzyloxy, acetyl, morpholinyl, -NR ₆ C(O)R ₇ , -C(O)NR ₆ R ₇ . and phenyl optionally substituted with halogen; R ₅ is hydrogen or a C _1-3 alkyl group; and R ₇ is a Cwalkyl group optionally substituted with phenyl, a phenyl, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl. In some embodiments, R ₄ is a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzofuranyl, indolinyl, benzimidazolyl, benzothiophenyl, benzothiazolyl, furo[3,2-c]pyridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, cyano, nitro, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, benzyloxy, acetyl, morpholinyl, - NR ₆ C(O)R ₇ , and -C(O)NR ₆ R ₇ In some embodiments, R ₄ is a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzofuranyl, indolinyl, benzimidazolyl, benzothiophenyl, benzothiazolyl, furo[3,2-c]pyridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, cyano, nitro, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, benzyloxy, acetyl, morpholinyl, - NR ₆ ,C(O)R ₇ . and -C(O)NR ₆ ,R ₇ : R ₆ is hydrogen or a C _1-3 alkyl group; and R ₇ is a C _1-3 alkyl group optionally substituted with phenyl, a phenyl, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0132] In some embodiments, R ₄ is quinolinyl or isoquinolinyl, each of which is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, cyano, nitro, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, benzyloxy, acetyl, morpholinyl, -NR ₆ C(O)R ₇ , and - C(O)NR ₆ R ₇ : R ₆ is hydrogen or a C _1-3 alkyl group; and R ₇ is a C _1-3 alkyl group optionally substituted with phenyl, a phenyl, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl. In some embodiments, R ₄ quinolinyl, optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, cyano, nitro, halogen, C _{1- 5} alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, benzyloxy, acetyl, morpholinyl, -NR ₆ C(O)R ₇ , and -C(O)NR ₆ R ₇ ; R ₆ is hydrogen or a C _1- 3alkyl group; and R ₇ is a C _1-3 alkyl group optionally substituted with phenyl, a phenyl, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0133] In some embodiments, R ₄ is a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, ftiro[3,2-c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, and acridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _{1- 5} alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxycarbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, and C _{1- 5} alkylsulfonyl. In some embodiments, R ₄ is a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, quinazolinyl, benzofuranyl, benzothiophenyl, furo[3,2- c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, and acridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, and phenyl optionally substituted with halogen. In some embodiments, R ₄ is a heteroaryl selected from the group consisting of quinolinyl, isoquinolinyl, benzofuranyl, benzothiophenyl, and furo[3,2-c]pyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, and C _1-5 alkoxy optionally substituted with 1 to 3 halogens.

[0134] In some embodiments, R ₄ is a heteroaryl selected from the group consisting of quinolinyl, isoquinolinyl, benzofuranyl, and benzothiophenyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, halogen, C _1-5 alkyl. and C _1-5 alkoxy.

[0135] In some embodiments, R ₄ is a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, quinazolinyl, thieno[2,3-d]pyrimidinyl, isoxazolyl, and acridinyl wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of cyano, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _{1- 5} alkoxy optionally substituted with 1 to 3 halogens, benzyloxy, and phenyl optionally substituted with halogen. In some embodiments, R ₄ is a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, quinazolinyl, thieno[2,3-d]pyrimidinyl, isoxazolyl, and acridinyl wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, benzyloxy, and phenyl optionally substituted with halogen.

[0136] In some embodiments, R ₄ is quinolinyl, isoquinolinyl, or quinazolinyl, each of which is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of cyano, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, and C _1-5 alkoxy optionally substituted with 1 to 3 halogens. In some embodiments, R ₄ is quinolinyl, isoquinolinyl, or quinazolinyl, each of which is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, and C _1-5 alkoxy optionally substituted with 1 to 3 halogens. [0137] In some embodiments, R ₄ is quinolinyl or isoquinolinyl, each of which is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, and C _1-5 alkoxy optionally substituted with 1 to 3 halogens.

[0138] In some embodiments, R ₄ is quinolinyl or isoquinolinyl, each of which is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, halogen, C _1-5 alkyl, and C _1-5 alkoxy. In some embodiments, R ₄ quinolinyl, optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, and C _1-5 alkoxy optionally substituted with 1 to 3 halogens.

[0139] In some embodiments, R ₄ is benzofuranyl, benzothiophenyl, or furo[3,2-c]pyridinyl, each of which is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of halogen, C _1-5 alkyl. and C _1-5 alkoxy. In some embodiments, R ₄ is benzofuranyl or benzothiophenyl, each of which is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of halogen, C _1-5 alky l . and C _1-5 alkoxy. In some embodiments, R ₄ is benzofuranyl, optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, halogen, C _1-5 alkyl and C _1-5 alkoxy. In some embodiments, R ₄ benzofuranyl or benzothiophenyl.

[0140] In some embodiments, R ₄ is pyridinyl, wherein said R ₄ is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _{1- 5} alkylamino, cyano, nitro, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₆ C(O)R ₇ . and -C(O)NR ₆ R ₇ . In some embodiments, R ₄ is pyridinyl, wherein said R ₄ is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₁ C(O)R ₇ , and -C(O)NR ₆ R ₇ ; R ₆ is hydrogen or a C _1-3 alkyl group; and R ₇ is a C _1-3 alkyl group optionally substituted with phenyl, a phenyl, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0141] In some embodiments, R ₄ is chromenonyl, wherein said R ₄ is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _{1- 5} alkylamino, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₁ C(O)R ₇ , and -C(O)NR ₆ R ₇ . In some embodiments, R ₄ is chromenonyl, wherein said R ₄ is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _{1- 5} alkylamino, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₆ C(O)R ₇ , and -C(O)NR ₆ ,R ₇ : R ₆ is hydrogen or a C _1-3 alkyl group; and R ₇ is a C _1-3 alkyl group optionally substituted with phenyl, a phenyl, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0142] In some embodiments, R ₄ is quinazolinyl, wherein said R ₄ is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _{1- 5} alkylamino, cyano, nitro, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₆ C(O)R ₇ , and -C(O)NR ₆ R ₇ . In some embodiments, R ₄ is quinazolinyl, wherein said R ₄ is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₆ C(O)R ₇ , and -C(O)NR ₆ ,R ₇ : R ₆ is hydrogen or a C _1-3 alkyl group; and R ₇ is a C _1-3 alkyl group optionally substituted with phenyl, a phenyl, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0143] In some embodiments, R ₄ is benzothiophenyl, wherein said R ₄ is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _{1- 5} alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₁ C(O)R ₇ , and -C(O)NR ₆ R ₇ . In some embodiments, R ₄ is benzothiophenyl, wherein said R ₄ is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _{1- 5} alkylamino, cyano, nitro, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₁ C(O)R ₇ , and -C(O)NR ₆ R ₇ ; R ₆ is hydrogen or a Cwalkyl group; and R ₇ is a C _1-3 alkyl group optionally substituted with phenyl, a phenyl, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0144] In some embodiments, R ₄ is indolinyl, wherein said R ₄ is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _{1- 5} alkylamino, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl. morpholinyl, -NR ₁ C(O)R ₇ , and -C(O)NR ₆ R ₇ . In some embodiments, R ₄ is indolinyl, wherein said R ₄ is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₁ C(O)R ₇ , and -C(O)NR ₆ R ₇ ; R ₆ is hydrogen or a C _1-3 alkyl group; and R ₇ is a C _1-3 alkyl group optionally substituted with phenyl, a phenyl, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0145] In some embodiments, R ₄ is benzothiazolyl, wherein said R ₄ is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di- C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₆ C(O)R ₇ , and -C(O)NR ₆ R ₇ . In some embodiments, R ₄ is benzothiazolyl, wherein said R ₄ is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _{1- 5} alkylamino, cyano, nitro, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₁ C(O)R ₇ , and -C(O)NR ₆ R ₇ ; R ₆ is hydrogen or a C _1-3 alkyl group; and R ₇ is a C _1-3 alkyl group optionally substituted with phenyl, a phenyl, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0146] In some embodiments, R ₄ is furo[3,2-c]pyridinyl, wherein said R ₄ is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _{1- 5} alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₁ C(O)R ₇ , and -C(O)NR ₆ R ₇ . In some embodiments, R ₄ furo[3,2-c]pyridinyl, wherein said R ₄ is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _{1- 5} alkylamino, cyano, nitro, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₁ C(O)R ₇ , and -C(O)NR ₆ R ₇ ; R ₆ is hydrogen or a C _1-3 alkyl group; and R ₇ is a C _1-3 alkyl group optionally substituted with phenyl, a phenyl, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0147] In some embodiments, R ₄ is naphthyridinyl, wherein said R ₄ is optionally substituted with

1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di- C _1-5 alkylamino, cyano, nitro, halogen, C _1-5 alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₁ C(O)R ₇ , and -C(O)NR ₆ R ₇ . In some embodiments, R ₄ is naphthyridinyl, wherein said R ₄ is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, amino, di-C _{1- 5} alkylamino, cyano, nitro, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxy carbonyl, benzyloxy, phenyl optionally substituted with halogen, acetyl, C _1-5 alkylsulfonyl, morpholinyl, -NR ₁ C(O)R ₇ , and -C(O)NR ₆ R ₇ ; R ₆ is hydrogen or a C _1-3 alkyl group; and R ₇ is a C _1-3 alkyl group optionally substituted with phenyl, a phenyl, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0148] In some embodiments, R ₄ is substituted or unsubstituted pyridinyl. In some embodiments, R ₄ is substituted or unsubstituted pyrimidinyl. In some embodiments, R ₄ is substituted or unsubstituted pyrazinyl. In some embodiments, R ₄ is substituted or unsubstituted imidazolyl. In some embodiments, R ₄ is substituted or unsubstituted pyrazolyl. In some embodiments, R ₄ is substituted or unsubstituted triazolyl. In some embodiments, R ₄ substituted or unsubstituted oxazolyl. In some embodiments, R ₄ is substituted or unsubstituted thiazolyl. In some embodiments, R ₄ is substituted or unsubstituted furanyl. In some embodiments, R ₄ is substituted or unsubstituted pyrrolyl. In some embodiments, R ₄ is substituted or unsubstituted thiophenyl. In some embodiments, R ₄ is substituted or unsubstituted quinolinyl. In some embodiments, R ₄ substituted or unsubstituted isoquinolinyl. In some embodiments, R ₄ is substituted or unsubstituted chromenonyl. In some embodiments, R ₄ is substituted or unsubstituted quinazobnyl. In some embodiments, R ₄ is substituted or unsubstituted benzoxazolyl. In some embodiments, R ₄ is substituted or unsubstituted benzofuranyl. In some embodiments, R ₄ is substituted or unsubstituted benzothiophenyl. In some embodiments, R ₄ substituted or unsubstituted indolyl. In some embodiments, R ₄ substituted or unsubstituted indolinyl. In some embodiments, R ₄ is substituted or unsubstituted benzimidazolyl. In some embodiments, R ₄ is substituted or unsubstituted benzoxazolyl. In some embodiments, R ₄ is substituted or unsubstituted benzothiazolyl.

In some embodiments, R ₄ is substituted or unsubstituted indazolyl. In some embodiments, R ₄ is substituted or unsubstituted furo[3,2-c]pyridinyl. In some embodiments, R ₄ substituted or unsubstituted thieno[2,3-d]pyrimidinyl. In some embodiments, R ₄ substituted or unsubstituted thieno[3,2-c]pyridinyl. In some embodiments, R ₄ substituted or unsubstituted isoxazolyl. In some embodiments, R ₄ is substituted or unsubstituted acridinyl. In some embodiments, R ₄ is substituted or unsubstituted naphthyridinyl.

[0149] In some embodiments, R ₄ is a phenyl substituted with a substituent selected from the group consisting of piperazinyl optionally substituted with acetyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, 2,3- dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2-oxo-1,2,3,4-tetrahydroquinolinyl; a naphthyl optionally substituted with halogen; or a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzofuranyl, indolinyl, benzimidazolyl, benzothiophenyl, benzothiazolyl, furo[3,2-c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, acridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, cyano, nitro, halogen, C _{1- 5} alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxycarbonyl, benzyloxy, acetyl, morpholinyl, -NR ₆ C(O)R ₇ . -C(O)NR ₆ R ₇ . and phenyl optionally substituted with halogen; wherein R ₆ is hydrogen or a C _1-3 alkyl group; and

R ₇ is a C _1-3 alkyl group optionally substituted with phenyl, a phenyl, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl. [0150]

In some embodiments, R ₄ is a phenyl substituted with a substituent selected from the group consisting of piperazinyl optionally substituted with acetyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; a heterocycle selected from the group consisting of benzo[d][1,3]dioxolyl, 2,3- dihydrobenzofuranyl, 2-oxo-1,2-dihydroquinolinyl, and 2-oxo-1,2,3,4-tetrahydroquinolinyl; a naphthyl optionally substituted with halogen; or a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, quinazolinyl, benzofuranyl, benzothiophenyl, furo[3,2-c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, and acridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, halogen, C _{1- 5} alkyl optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxycarbonyl, benzyloxy, and phenyl optionally substituted with halogen.

[0151] In some embodiments, R ₄ is a phenyl substituted with phenyl; a naphthyl; or a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzofuranyl, indolinyl, benzimidazolyl, benzothiophenyl, benzothiazolyl, furo[3,2-c]pyridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, cyano, nitro, halogen, C _1-5 alkyl optionally substituted 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, benzyloxy, acetyl, morpholinyl, -NR ₁ C(O)R ₇ , and -C(O)NR ₆ R ₇ .

[0152] In some embodiments, R ₄ is a phenyl substituted with phenyl; a naphthyl; or a heteroaryl selected from the group consisting of quinolinyl, isoquinolinyl, benzofuranyl, benzothiophenyl, and furo[3,2-c]pyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of halogen, C _1-5 alkyl optionally substituted 1 to 3 halogens, and C _1-5 alkoxy optionally substituted with 1 to 3 halogens.

[0153] In some embodiments, R ₅ is hydrogen. In some embodiments, R ₅ is a C _1-3 alkyl.

[0154] In some embodiments, R ₆ is hydrogen. In some embodiments, R ₆ is a C _1-3 alkyl group.

[0155] In some embodiments, R ₇ is a C _1-3 alkyl group optionally substituted with phenyl.

[0156] In some embodiments, R ₇ is a phenyl optionally substituted with 1 to 3 Z ² ; wherein each Z ² is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, hydroxycarbonyl, C _1-5 alkyl optionally substituted by 1 to 3 Z ³ , C _2-6 alkenyl, C2- 6alkynyl, C _1-5 alkoxy optionally substituted byl to 3 Z ³ , C _1-5 alky lthio. mono- or di-C _{1- 5} alkylamino, piperazinyl optionally substituted by Z ³ , C _1-5 alkylsulfonylamino, C _{1- 5} alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _1-5 alkylaminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; wherein each Z ³ is independently chosen from halogen, amino, or acetyl

[0157] In some embodiments, R ₇ is a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, pyrazinyl, imidazolyl, pyrazolyl, triazolyl, oxazolyl, thiazolyl, furanyl, pyrrolyl, thiophenyl, quinolinyl, isoquinolinyl, chromenonyl, quinazolinyl, benzoxazolyl, benzofuranyl, benzothiophenyl, indolyl, indolinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indazolyl, furo[3,2-c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, acridinyl, and naphthyridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z ² ; wherein each Z ² is independently selected from the group consisting of hydroxy, amino, halogen, cyano, nitro, hydroxycarbonyl, C _1-5 alkyl optionally substituted byl to 3 Z ³ , C _2-6 alkenyl, C _{2- 6} alkynyl, C _1-5 alkoxy optionally substituted byl to 3 Z ³ , C _1-5 alkylthio, mono- or di-C _{1- 5} alkylamino, piperazinyl optionally substituted by Z ³ , C _1-5 alkylsulfonylamino, C _{1- 5} alky1carbonylamino, aminosulfonyl, aminocarbonyl, C _1-5 alkylaminocarbonyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; wherein each Z ³ is independently chosen from halogen, amino, or acetyl.

[0158] In some embodiments, R ₅ is hydrogen or a C _1-3 alkyl group; and R ₇ is a C _1-3 alkyl group optionally substituted with phenyl, a phenyl, or a heteroaryl selected from the group consisting of pyridinyl, pyrimidinyl, and isoxazolyl.

[0159] In one embodiment of the present technology, there is provided a compound of Formulas X, Xa, Xb, 1, 1a, 1b, 1c, 1d, or 1e, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, wherein n is 1 or 2;

R ₁ is a substituent selected from the group consisting of Formulas I, II, III, and V;

R ₂ is hydrogen;

R ₃ is a substituent selected from the group consisting of -NR ₄ R ₅ , -OR ₄ , -C(O)NHR ₄ , - NHC(O)(CH ₂ ) _m R ₄ , and -NHS(O) ₂ R ₄ , wherein m is 0 or 1; R ₄ is a phenyl substituted with a substituent selected from the group consisting of piperazinyl optionally substituted with acetyl, phenyl, phenoxy, benzyl, benzoyl, phenylaminocarbonyl, pyrazolyl, and benzoxazolyl; a naphthyl optionally substituted with halogen; or a heteroaryl selected from the group consisting of pyridinyl, quinolinyl, isoquinolinyl, 2-oxo- 1,2-dihydroquinolinyl, 2-oxo-1,2,3,4-tetrahydroquinolinyl, quinazolinyl, benzofuranyl, 2,3- dihydrobenzofuranyl, benzothiophenyl, benzo[d][1,3]dioxolyl, furo[3,2-c]pyridinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-c]pyridinyl, isoxazolyl, and acridinyl, wherein said heteroaryl is optionally substituted with 1 to 3 Z, wherein each Z is independently selected from the group consisting of hydroxy, halogen, C _1-5 alky l optionally substituted with 1 to 3 halogens, C _1-5 alkoxy optionally substituted with 1 to 3 halogens, C _1-5 alkoxycarbonyl, benzyloxy, and phenyl optionally substituted with halogen), and

R ₅ is hydrogen.

[0160] In another aspect, a compound of Formulas X, Xa, Xb, 1, 1, 1a, 1b, 1c, 1d, or 1e, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof is selected from the following compounds, or stereoisomers thereof, or a pharmaceutically acceptable salt thereof:

(R)- 1 -(2-((S)-2-cyanopyrrolidin- 1 -yl)-2-oxoethyl)-N-(quinolin-3 -yl)pyrrolidine-3 - carboxamide;

(R)-1-(2-((S)-2-cyanopynOlidin-1-yl)-2-oxoethyl)-N-(quino lin-4-yl)pyrrolidine-3- carboxamide;

(S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)-N-(quin olin-3-yl)pyrrolidine-3- carboxamide;

(S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)-N-(quin olin-4-yl)pyrrolidine-3- carboxamide;

(S)- 1 -(2-((S)-3-(quinolin-4-ylamino)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2-carbonitrile;

(S)-4,4-difluoro-1-(2-((S)-3-(quinolin-3-ylamino)pyrrolid in-1-yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-4,4-difluoro- 1 -(2-((S)-3 -(quinolin-4-ylamino)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-4,4-difluoro- 1 -(2-((S)-3 -(isoquinolin-4-ylamino)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-1-(2-((S)-3-(benzo[b]thiophen-4-ylamino)pyrrolidin-1- yl)acetyl)-4,4- difluoropyrrolidine-2-carbonitrile;

(S)- 1 -(2-((S)-3-(benzo[b]thiophen-7-ylamino)pyrrolidin- 1 -yl)acetyl)-4,4- difluoropyrrolidine-2-carbonitrile;

(S)-1-(2-((S)-3-(benzofuran-7-ylamino)pyrrolidin-1-yl)ace tyl)-4,4-difluoropyrrolidine-2- carbonitrile;

(S)-4,4-difluoro- 1 -(2-((S)-3 -(furo [3 ,2-c]pyridin-7-ylamino)pyrrolidin- 1 - yl)acetyl)pyrrolidine-2-carbonitrile;

(S)- 1 -(2-((S)-3 -( [ 1 , 1 '-biphenyl] -2-ylamino)pyrrolidin- 1 -yl)acetyl)-4,4-difluoropyrrolidine-2- carbonitrile;

(S)- 1 -(2-((S)-3 -( [ 1 , 1 '-biphenyl] -4-ylamino)pyrrolidin- 1 -yl)acetyl)-4,4-difluoropyrrolidine-2- carbonitrile;

(S)-4,4-difluoro-1-(2-((S)-3-(naphthalen-2-ylamino)pyrrol idin-1-yl)acetyl)pyrrolidine-2- carbonitrile;

(S)- 1 -(2-((S)-3 -( [ 1 , 1 '-biphenyl] -3 -ylamino)pyrrolidin- 1 -yl)acetyl)-4,4-difluoropyrrolidine-2- carbonitrile; (S)-1-(2-((R)-3-(quinolin-3-ylamino)pyrrolidin-1-yl)acetyl)p yrrolidine-2-carbonitrile;

(S)- 1 -(2-((R)-3-([ 1 , 1 '-biphenyl] -3 -ylamino)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2-carbonitrile ;

(S)- 1 -(2-((S)-3 -(isoquinolin-4-ylamino)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2-carbonitrile;

(S)-1-(2-((S)-3-(benzo[b]thiophen-4-ylamino)pyrrolidin-1- yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-1-(2-((S)-3-(benzo[b]thiophen-7-ylamino)pyrrolidin-1- yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-1-(2-((S)-3-(benzofuran-7-ylamino)pyrrolidin-1-yl)ace tyl)pyrrolidine-2-carbonitrile;

(S)- 1 -(2-((S)-3 -(f iro [3 ,2-c]pyridin-7 -ylamino)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2- carbonitrile;

(S)- 1 -(2-((R)-3 -(isoquinolin-4-ylamino)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2-carbonitrile;

(S)-1-(2-((R)-3-(benzo[b]thiophen-7-ylamino)pyrrolidin-1- yl)acetyl)pyrrolidine-2- carbonitrile;

( 1 S,3 S,5 S)-2-(2-((S)-3 -(fiiro [3 ,2-c]pyridin-7 -ylamino)pyrrolidin- 1 -yl)acetyl)-2- azabicyclo[3.1.0]hexane-3-carbonitrile;

(S)-1-(2-((S)-3-(quinolin-5-ylamino)pynOlidin-1-yl)acetyl )pyrrolidine-2-carbonitrile;

(2S,4S)-4-fluoro-1-(2-((S)-3-(furo[3,2-c]pyridin-7-ylamin o)pyrrolidin-1- yl)acetyl)pyrrolidin-2-carbonitrile;

N-((S)-1-(2-((2S,4S)-2-cyano-4-fluoropyrrolidin-1-yl)-2-o xoethyl)pyrrolidin-3-yl)-6- fluorobenzofuran-3 -carboxamide ;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)quinoline-4- carboxamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)quinoline-3- carboxamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)quinoline-5- carboxamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)quinoline-2- carboxamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)quinoline-8- carboxamide; N-((S)- 1 -(2-((S)-2-cyanopyrrolidin- 1 -yl)-2-oxoethyl)pyrrolidin-3-yl)isoquinoline- 1 - carboxamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)benzo[b]thiophene-2- carboxamide;

N-((S)- 1 -(2-((S)-2-cyanopyrrolidin- 1 -yl)-2-oxoethyl)pyrrolidin-3-yl)benzofuran-3- carboxamide;

N-((S)- 1 -(2-((S)-2-cyanopyrrolidin- 1 -yl)-2-oxoethyl)pyrrolidin-3-yl)-2-(naphthalen- 1 - yl)acetamide;

N-((S)- 1 -(2-((S)-2-cyanopyrrolidin- 1 -yl)-2-oxoethyl)pyrrolidin-3-yl)-[ 1 , 1 '-biphenyl]-2- carboxamide;

N-((S)- 1 -(2-((S)-2-cyanopyrrolidin- 1 -yl)-2-oxoethyl)pyrrolidin-3-yl)benzofuran-2- carboxamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)benzo[b]thiophene-3- carboxamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)-2-methylbenzofuran- 3 -carboxamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)-5-fluorobenzofuran-3- carboxamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)-6-fluorobenzofuran-3- carboxamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)-7-methylbenzofuran- 3 -carboxamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)-5-methoxybenzofuran- 3 -carboxamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)-7-methoxybenzofuran- 3 -carboxamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)-4-hydroxybenzofuran- 3 -carboxamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)-6-hydroxybenzofuran- 3 -carboxamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)-7-fluorobenzofuran-3- carboxamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)-5,7- difluorobenzofuran-3 -carboxamide ;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)-5-methylbenzofuran- 3 -carboxamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)-6-methylbenzofuran- 3 -carboxamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)-6-methoxybenzofuran- 3 -carboxamide;

N-((S)- 1 -(2-((S)-2-cyanopyrrolidin- 1 -yl)-2-oxoethyl)pyrrolidin-3-yl)benzofuran-4- carboxamide;

N-((S)- 1 -(2-((S)-2-cyanopyrrolidin- 1 -yl)-2-oxoethyl)pyrrolidin-3-yl)benzofuran-5- carboxamide;

N-((S)- 1 -(2-((S)-2-cyanopyrrolidin- 1 -yl)-2-oxoethyl)pyrrolidin-3-yl)benzofuran-6- carboxamide;

N-((S)- 1 -(2-((S)-2-cyanopyrrolidin- 1 -yl)-2-oxoethyl)pyrrolidin-3-yl)benzofuran-7- carboxamide;

N-((S)- 1 -(2-((S)-2-cyanopyrrolidin- 1 -yl)-2-oxoethyl)pyrrolidin-3-yl)benzofuran-2- sulfonamide;

N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrro lidin-3-yl)benzo[b]thiophene-2- sulfonamide;

(S)-1-(2-((S)-3-(quinolin-4-yloxy)pyrrolidin-1-yl)acetyl) pyrrolidine-2-carbonitrile;

(S)- 1 -(2-((S)-3 -(naphthalen- 1 -yloxy)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2-carbonitrile;

(S)-1-(2-((S)-3-(quinolin-6-yloxy)pyrrolidin-1-yl)acetyl) pyrrolidine-2-carbonitrile;

(S)-1-(2-((S)-3-(quinolin-8-yloxy)pyrrolidin-1-yl)acetyl) pyrrolidine-2-carbonitrile;

(S)-1-(2-((S)-3-(isoquinolin-5-yloxy)pyrrolidin-1-yl)acet yl)pyrrolidine-2-carbonitrile;

(S)-1-(2-((S)-3-(quinolin-5-yloxy)pyrrolidin-1-yl)acetyl) pyrrolidine-2-carbonitrile;

(S)- 1 -(2-((S)-3 -(benzo [d] [ 1 ,3]dioxol-5 -yloxy)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-1-(2-((S)-3-(thieno[2,3-d]pyrimidin-4-yloxy)pynOlidin -1-yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-1-(2-((S)-3-(isoquinolin-3-yloxy)pyrrolidin-1-yl)acet yl)pyrrolidine-2-carbonitrile;

(S)-1-(2-((S)-3-((3-bromothieno[3,2-c]pyridin-4-yl)oxy)py rrolidin-1-yl)acetyl)pyrrolidine-

2-carbonitrile;

(S)- 1 -(2-((S)-3 -((4-chloronaphthalen- 1 -yl)oxy)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2- carbonitrile;

(S)- 1 -(2-((S)-3 -(2-( 1H-pyrazol-3 -yl)phenoxy)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-1-(2-((S)-3-((4-chloroquinazolin-8-yl)oxy)pynOlidin-1 -yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-1-(2-((S)-3-((4-chlorc>quinazolin-6-yl)oxy)pyrroli din-1-yl)acetyl)pyrrolidine-2- carbonitrile;

(S)- 1 -(2-((S)-3-((2-oxo- 1 ,2-dihydroquinolin-5 -yl)oxy)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2- carbonitrile;

(S)- 1 -(2-((S)-3-((2-oxo- 1 ,2,3 ,4-tetrahydroquinolin-5 -yl)oxy)pyrrolidin- 1 - yl)acetyl)pyrrolidine-2-carbonitrile;

(S)- 1 -(2-((S)-3-((2-oxo- 1 ,2,3 ,4-tetrahydroquinolin-6-yl)oxy)pyrrolidin- 1 - yl)acetyl)pyrrolidine-2-carbonitrile;

(S)-1-(2-((S)-3-((2-methylquinolin-8-yl)oxy)pyrrolidin-1- yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-1-(2-((S)-3-((7-chloroquinolin-4-yl)oxy)pyrrolidin-1- yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-1-(2-((S)-3-((7-bromoquinolin-4-yl)oxy)pynOlidin-1-yl )acetyl)pyrrolidine-2- carbonitrile;

(S)-1-(2-((S)-3-((5-chloroquinolin-8-yl)oxy)pyrrolidin-1- yl)acetyl)pyrrolidine-2- carbonitrile;

(S)- 1 -(2-((S)-3 -([ 1 , 1 '-biphenyl] -3 -yloxy)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2 -carbonitrile;

(S)- 1 -(2-((S)-3 -([ 1 , 1 '-biphenyl] -4-yloxy)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2 -carbonitrile;

(S)-1-(2-((S)-3-((6-methoxyquinolin-4-yl)oxy)pyrrolidin-1 -yl)acetyl)pyrrolidine-2- carbonitrile; (S)-1-(2-((S)-3-((7,8-difluoroquinolin-4-yl)oxy)pyrrolidin-1 -yl)acetyl)pyrrolidine-2- carbonitrile; methyl 6-(((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrroli din-3-yl)oxy)-2,3- dihy drobenzofuran-2-carboxy late ;

(S)- 1 -(2-((S)-3 -(4-phenoxyphenoxy)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2 -carbonitrile;

(S)-1-(2-((S)-3-(3-phenoxyphenoxy)pyrrolidin-1-yl)acetyl) pyrrolidine-2 -carbonitrile;

(S)-1-(2-((S)-3-(4-benzylphenoxy)pynOlidin-1-yl)acetyl)py rrolidine-2-carbonitrile;

(S)-1-(2-((S)-3-(2-benzylphenoxy)pynOlidin-1-yl)acetyl)py rrolidine-2-carbonitrile;

(S)-1-(2-((S)-3-(3-benzoylphenoxy)pyrrolidin-1-yl)acetyl) pyrrolidine-2-carbonitrile;

(S)- 1 -(2-((S)-3 -(4-benzoylphenoxy)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2-carbonitrile;

(S)- 1 -(2-((S)-3 -((4-(benzyloxy)pyridin-2-yl)oxy)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-1-(2-((S)-3-((7-(trifluoromethyl)quinolin-4-yl)oxy)py rrolidin-1-yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-1-(2-((S)-3-((8-(trifluoromethyl)quinolin-4-yl)oxy)py rrolidin-1-yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-1-(2-((S)-3-(acridin-4-yloxy)pyrrolidin-1-yl)acetyl)p yrrolidine-2-carbonitrile;

(S)-1-(2-((S)-3-(2-(benzo[d]oxazol-2-yl)phenoxy)pyrrolidi n-1-yl)acetyl)pyrrolidine-2- carbonitrile;

(S)- 1 -(2-((S)-3 -(4-(4-acetylpiperazin- 1 -yl)phenoxy)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2- carbonitrile;

2-(((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrr olidin-3-yl)oxy)-N- pheny1benzamide ;

(S)- 1 -(2-((S)-3-((7-methoxyquinolin-4-yl)oxy)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-1-(2-((S)-3-((6,7-dimethoxyquinolin-4-yl)oxy)pyrrolid in-1-yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-1-(2-((S)-3-((3-(4-bromophenyl)isoxazol-5-yl)oxy)pyrr olidin-1-yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-4,4-difluoro- 1 -(2-((S)-3 -((6-methoxyquinolin-4-yl)oxy)pyrrolidin- 1 - yl)acetyl)pyrrolidine-2-carbonitrile;

(S)-4,4-difluoro-1-(2-((S)-3-((8-(trifluoromethyl)quinoli n-4-yl)oxy)pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile;

(S)-4,4-difluoro-1-(2-((S)-3-(quinolin-5-yloxy)pynOlidin- 1-yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-4,4-difluoro-1-(2-((S)-3-(isoquinolin-5-yloxy)pyrroli din-1-yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-1-(2-((S)-3-((7-chloroquinolin-4-yl)oxy)pyrrolidin-1- yl)acetyl)-4,4-difluoropyrrolidine-

2-carbonitrile;

(S)-4,4-difluoro- 1 -(2-((S)-3 -((2-oxo- 1 ,2-dihydroquinolin-5 -yl)oxy)pyrrolidin- 1 - yl)acetyl)pyrrolidine-2-carbonitrile;

(S)-1-(2-((S)-3-((5-chloroquinolin-8-yl)oxy)pyrrolidin-1- yl)acetyl)-4,4-difluoropyrrolidine-

2-carbonitrile;

(S)-1-(2-(4-(quinolin-5-ylamino)piperidin-1-yl)acetyl)pyr rolidine-2-carbonitrile;

(S)- 1 -(2-(4-(quinolin-4-ylamino)piperidin- 1 -yl)acetyl)pyrrolidine-2-carbonitrile ;

(15.35.55)-2-(2-(4-(quinolin-4-ylamino)piperidin-1-yl)ace tyl)-2-azabicyclo[3.1.0]hexane-3- carbonitrile;

(2S,4S)-4-fluoro-1-(2-(4-(quinolin-5-ylamino)piperidin-1- yl)acetyl)pyrrolidine-2- carbonitrile;

(S)-4,4-difluoro- 1 -(2-(4-(quinolin-5 -ylamino)piperidin- 1 -yl)acetyl)pyrrolidine-2- carbonitrile;

(15.35.55)-2-(2-(4-(quinolin-5-ylamino)piperidin-1-yl)ace tyl)-2-azabicyclo[3.1.0]hexane-3- carbonitrile;

(S)- 1 -(2-(4-(furo [3 ,2-c]pyridin-7 -ylamino)piperidin- 1 -yl)acetyl)pyrrolidine-2-carbonitrile;

(S)-1-(2-(4-(quinolin-3-yloxy)piperidin-1-yl)acetyl)pyrro lidine-2 -carbonitrile;

(S)- 1 -(2-(4-(quinolin-4-yloxy)piperidin- 1 -yl)acetyl)pyrrolidine-2 -carbonitrile;

(S)-1-(2-(4-(quinolin-5-yloxy)piperidin-1-yl)acetyl)pyrro lidine-2 -carbonitrile;

(S)- 1 -(2-(4-(quinolin-6-yloxy)piperidin- 1 -yl)acetyl)pyrrolidine-2 -carbonitrile;

(S)-1-(2-(4-((3-methylquinolin-5-yl)amino)piperidin-1-yl) acetyl)pyrrolidine-2 -carbonitrile; (S)-1-(2-(4-((6-methylquinolin-5-yl)amino)piperidin-1-yl)ace tyl)pyrrolidine-2-carbonitrile;

(S)-1-(2-(4-((7-fluoroquinolin-5-yl)amino)piperidin-1-yl) acetyl)pyrrolidine-2-carbonitrile;

(S)- 1 -(2-(4-((7-methoxyquinobn-5 -yl)amino)piperidin- 1 -yl)acetyl)pyrrolidine-2- carbonitrile;

(S)- 1 -(2-(4-((8-methoxyquinobn-5 -yl)amino)piperidin- 1 -yl)acetyl)pyrrolidine-2- carbonitrile;

(25.45)-4-fluoro-1-(2-(4-((6-fluoroquinolin-4-yl)oxy)pipe ridin-1-yl)acetyl)pyrrolidine-2- carbonitrile;

(25.45)-1-(2-(4-((6-chloroquinolin-4-yl)oxy)piperidin-1-y l)acetyl)-4-fluoropyrrolidine-2- carbonitrile;

(25.45)-4-fluoro-1-(2-(4-((6-methoxyquinolin-4-yl)oxy)pip eridin-1-yl)acetyl)pyrrolidine-2- carbonitrile;

(25.45)-4-fluoro-1-(2-(4-((7-methoxyquinolin-4-yl)oxy)pip eridin-1-yl)acetyl)pyrrolidine-2- carbonitrile;

(25.45)-1-(2-(4-((6,7-dimethoxyquinolin-4-yl)oxy)piperidi n-1-yl)acetyl)-4- fluoropyrrolidine-2-carbonitrile;

(25.45)-4-fluoro-1-(2-(4-((3-methylquinolin-5-yl)amino)pi peridin-1-yl)acetyl)pyrrolidine-2- carbonitrile;

(25.45)-4-fluoro-1-(2-(4-((7-fluoroquinobn-5-yl)amino)pip eridin-1-yl)acetyl)pyrrolidine-2- carbonitrile;

(25.45)-4-fluoro-1-(2-(4-((7-methoxyquinolin-5-yl)amino)p iperidin-1-yl)acetyl)pyrrolidine- 2-carbonitrile;

(25.45)-4-fluoro-1-(2-(4-((6-methylquinolin-5-yl)amino)pi peridin-1-yl)acetyl)pyrrolidine-2- carbonitrile; and

(25.45)-4-fluoro-1-(2-(4-((8-methoxyquinolin-5-yl)amino)p iperidin-1-yl)acetyl)pyrrolidine- 2-carbonitrile.

[0161] In another aspect, a compound of Formulas X, Xa, Xb, 1, 1, 1a, 1b, 1c, 1d, or 1e, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof is selected from the compounds listed in Table 1, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof.

[0162] The compound of Formulas X, Xa, Xb, 1, 1a, 1b, 1c, 1d, or 1e, or the pharmaceutically acceptable salt thereof can have a substituent comprising asymmetric carbon (e.g., a substituent of

R ₂ ). In this case, the compound of Formula 1 or a salt thereof may exist as an optical isomer, such as (R) or (S), or a racemate (RS). Thus, unless indicated otherwise, the compound of Formula 1 or a salt thereof comprises both an optical isomer, such as (R) or (S), and a racemate (RS). Further, in some embodiments, the compound of Formula 1 or a salt thereof may exist as the geometric isomer of a double-bond cis- or trans-structure depending on the substituent. Thus, in some embodiments, unless indicated otherwise, the compound of Formula 1 or salt thereof comprises both geometric isomers of cis- and trans-structures. Further, the compound of Formula 1 or a salt thereof may exist as a diastereomer and, unless indicated otherwise, comprises both diastereomers and mixtures thereof.

[0163] The compounds provided in the description are inhibitors of FAR FAP inhibition may be measured, for example, by determining the half maximal inhibitory concentration (IC50). One method for determining an IC50 for FAP is provided in the experimental section.

[0164] In some embodiments, the compounds are selective inhibitors of FAR Selectivity may be determined, for example, by comparing inhibition of FAP to inhibition to other DPP families such as DPP7 (dipeptidyl peptidase 7), DPP8 (dipeptidyl peptidase 8), DPP9 (dipeptidyl peptidase 9), or PREP (prolyl oligopeptidase). In one embodiment, "selective inhibitory activity" means IC50 for FAP obtained from the in vitro enzyme analysis (in vitro enzyme assay) test is at least 100 times lower than IC50 of DPP7, at least 100 times lower than IC50 of DPP8, at least 100 times lower than IC50 of DPP9, or at least 100 times lower than IC50 of PREP In an alternative embodiment, "selective inhibitory activity" means the IC50 for FAP obtained from the in vitro enzyme analysis (in vitro enzyme assay) test is at least 100 times lower than IC50 of DPP7, at least 100 times lower than IC50 of DPP8, at least 100 times lower than IC50 of DPP9, and at least 100 times lower than IC50 of PREP.

[0165] The compound of Formula 1 of the present technology can be in the form of a pharmaceutically acceptable salt. The term "pharmaceutically acceptable salt," as used herein, represents salts or zwitterionic forms of the compounds of the present technology which are water or oil-soluble or dispersible; which are suitable for treatment of diseases without undue toxicity, irritation, and allergic-response; which are commensurate with a reasonable benefit/risk ratio; and which are effective for their intended use. The salts can be prepared during the final isolation and purification of the compounds or separately by, for example, reacting the appropriate compound in the form of the free base with a suitable acid. Such salts include conventional acid addition salts, e.g., a salt derived from inorganic acid such as hydrochloric acid, bromic acid, sulfuric acid, sulfamic acid, phosphoric acid, or nitric acid and a salt derived from organic acid such as acetic acid, propionic acid, succinic acid, glycolic acid, stearic acid, citric acid, maleic acid, malonic acid, methanesulfonic acid, tartaric acid, malic acid, phenylacetic acid, glutamic acid, benzoic acid, salicylic acid, 2- acetoxybenzoic acid, fumaric acid, p-toluenesulfonic acid, oxalic acid or trifluoroacetic acid. Further, said salts include conventional metal salt types, e.g. a salt derived from a metal such as lithium, sodium, potassium, magnesium, or calcium. Said acid addition salt or metal salt can be prepared according to conventional methods.

[0166] The compound of Formula 1 or a salt thereof according to the technology may be prepared by various methods. For example, a compound of Formula la wherein R ₃ of Formula 1 is -NR ₄ R ₅ , or a salt thereof can be prepared by a preparation process comprising the step of reacting a compound of Formula 2a with R ₄ X to prepare a compound of Formula 3a; the step of conducting deprotection reaction of the compound of Formula 3a above to prepare a compound of Formula 4a or a salt thereof; and the step of reacting the compound of Formula 4a above or a salt thereof with a compound of Formula 5 to form a compound of Formula la.

In said Formulas 1a, 2a, 3a, 4a, and 5, X is a halogen (e.g., Br, F, Cl, Br, or I), Boc is an amine protecting group (e.g., tert-butoxycarbonyl, 9-fluorenylmethyloxycarbonyl (FMOC), benzyloxycarbonyl (CBZ), triphenylmethyl(trityl), etc.), and R ₁ , R ₂ , R ₄ , R ₅ , and n are the same as defined above. [0167] The compound of Formula 2a is commercially available. The reaction of the compound of Formula 2a above with R ₄ X may be carried out by using a palladium catalyst via Buchwald-Hartwig reaction. The palladium catalyst includes palladium diacetate (Pd(OAc) ₂ ), tris(dibenzylideneacetone)dipalladium (Pd ₂ (dba) ₃ ), tetrakis(triphenylphosphine)palladium (Pd(PPh ₃ ) ₄ ) or palladiumdi[1, 1'-bis(diphenylphosphino)fenOcene]dichloride (PdCI ₂ (dppf) ₂ ), etc. Further, a ligand and a base can be added in addition to the palladium catalyst. Said ligand includes (S)-2,2- bis(diphenylphospino)- 1,1 -binaphthyl (BINAP), 1,1'-bis(diphenylphospino)ferrocene(dppf) or (tri-O- tolyl)phosphine(P(O-Tol) ₃ ), etc., and said base includes an inorganic base such as cesium carbonate (Cs2CO ₃ ), sodium carbonate (Na ₂ CO ₃ ), potassium carbonate (K ₂ CO ₃ ) potassium fluoride (KF), cesium fluoride (CsF), sodium hydroxide (NaOH), potassium phosphonate (K3PO4), sodium tert- butoxide (tert-BuONa), potassium tert-butoxide (tert-BuOK), or the like. The reaction may be carried out, in a non-polar organic solvent such as benzene or toluene, or a polar organic solvent such as dioxane, tetrahydrofuran, acetonitrile, 1,2-dimethoxyethane, N,N-dimethylformamide, or the like, at a temperature ranging from 50°C to 150°C, preferably from 80°C to 110°C. Other reaction conditions, including e.g., reaction time, may be determined according to known methods for the Buchwald- Hartwig reaction (Barbara Czako et al, STRATEGIC APPLICATIONS of NAMED REACTIONS in ORGANIC SYNTHESIS, 2005).

[0168] In some embodiments, the reaction of the compound of Formula 2a with R ₄ X is carried out via nucleophilic substitution. The nucleophilic substitution reaction may be carried out at room temperature or reflux conditions (20 °C to 120 °C) by using a base such as cesium carbonate, potassium carbonate, etc., in a solvent such as acetonitrile, dichloromethane, or the like.

[0169] Deprotection of the compound of Formula 3a can be carried out by conventional methods of removing an amine protecting group. For example, said amine protecting group in an organic solvent such as dichloromethane, etc. can be removed by using an acid such as trifluoroacetic acid or can be removed in the form of a hydrochloride salt by using hydrogen chloride dissolved in the organic solvents, such as diethyl ether, 1,4-dioxane, etc.

[0170] The reaction of the compound of Formula 4a above or a salt thereof with a compound of Formula 5 may be carried out via nucleophilic substitution. The nucleophilic substitution reaction can be carried out at room temperature or warm temperature conditions (20°C to 60°C) by using a base such as cesium carbonate, potassium carbonate, etc., in a solvent such as acetonitrile, dichloromethane, or the like.

[0171] The compound of Formula 5 above can be prepared by reacting the compound of Formula 6 with the compounds of Formulas R ₁ a-R ₁ e, respectively.

In said Formula 6, R ₂ is the same as defined in the above.

[0172] The compound of Formula 6 and the compounds of Formulas R ₁ a to R ₁ e are commercially available. The reaction of the compound of Formula 6 with the compounds of Formulas R ₁ a-R ₁ e can be converted to a compound of Formula 5 via nucleophilic acylsubstitution. The nucleophilic acyl- substitution reaction can be carried out by using cesium carbonate, potassium carbonate, etc., in a solvent such as acetonitrile, dichloromethane, or the like, and can be carried out at 0 °C to room temperature.

[0173] Further, the present technology includes a preparation process of a compound of Formula 1b wherein R ₃ is -OR ₄ , or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof. That is, the present technology includes a preparation process of a compound of Formula 1b, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, comprising the step of reacting a compound of Formula 2b with R ₄ -OH to prepare a compound of Formula 3b; the step of carrying out deprotection reaction of the compound of Formula 3b above to prepare a compound of Formula 4b or a salt thereof; and the step of reacting a compound of Formula 4b or a salt thereof with a compound of Formula 5 to give a compound of Formula 1b.

In said Formulas 1b, 2b, 3b, 4b, and 5, Boc is an amine protecting group (e.g., tert-butoxycarbonyl, 9- fluorenylmethyloxycarbonyl (FMOC), benzyloxycarbonyl(CBZ), triphenylmethyl(trityl), etc.) and R ₁ ,

R ₂ , R ₄ and n are the same as defined in the above.

[0174] The compound of Formula 2b is commercially available. The reaction of the compound of Formula 2b above with R ₄ -OH may be carried out via Mitsunobu reaction. The Mitsunobu reaction can be carried out at room temperature or warm temperature condidtions (20°C to 60°C) by using dialkyl azodicarboxylate such as diethyl azodicarboxylate (DEAD), diisopropyl azodicarboxylate (DIAD), di-2-methoxyethyl azodicarboxylate (DMEAD), 1,1'-(azodicarbonyl)dipiperidine, etc. and trialkylphosphine or triarylphosphine such as triphenylphosphine (PPh ₃ ), tributylphosphine (PBu ₃ ), etc. Other reaction conditions, including e.g., reaction time, may be determined according to known methods for the Mitsunobu reaction (Barbara Czako and Laszlo Kurti, STRATEGIC APPLICATIONS of NAMED REACTIONS in ORGANIC SYNTHESIS, 2005).

[0175] Deprotection of the compound of Formula 3b can be carried out by conventional methods of removing an amine protecting group. For example, said amine protecting group in an organic solvent such as dichloromethane, etc. can be removed by using an acid such as trifluoroacetic acid or can be removed in the form of a hydrochloride salt by using hydrogen chloride dissolved in the organic solvents, such as diethyl ether, 1,4-dioxane, etc.

[0176] The reaction of the compound of Formula 4b above or a salt thereof with a compound of Formula 5 may be carried out via nucleophilic substitution. The nucleophilic substitution reaction can be carried out at room temperature or warm temperature conditions (20°C to 60°C) by using a base such as cesium carbonate, potassium carbonate, etc., in a solvent such as acetonitrile, dichloromethane, or the like. [0177] The present technology includes a preparation process of a compound of Formula 1b, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, comprising the step of reacting a compound of Formula 5 with a compound of Formula 7 to prepare a compound of Formula 8; the step of reacting the compound of Formula 8 above with R ₄ -OH to prepare a compound of Formula 1b.

In said Formulas 1b, 5, 7, and 8, R ₁ , R ₂ , R ₄ , and n are the same as defined in the above.

[0178] The compound of Formula 7 is commercially available. The reaction of the compound of Formula 5 above with the compound of Formula 7 may be carried out via nucleophilic substitution. The nucleophilic substitution reaction can be carried out at room temperature or warm temperature conditions (20°C to 60°C) by using a base such as cesium carbonate, potassium carbonate, etc., in a solvent such as acetonitrile, dichloromethane, or the like.

[0179] The reaction of the compound of Formula 8 above with R ₄ -OH may be carried out via the Mitsunobu reaction.

[0180] Further, the present technology includes a preparation process of a compound of Formula 1c wherein R ₃ of Formula 1 is -C(O)NHR ₄ , or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof. That is, the present technology includes a preparation process of a compound of Formula 1c, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, comprising the step of reacting a compound of Formula 2c with R ₄ -NH ₂ to prepare a compound of Formula 3c; the step of carrying out deprotection reaction of the compound of Formula 3c above to prepare a compound of Formula 4c or a salt thereof; and the step of reacting the compound of Formula 4c or a salt thereof with a compound of Formula 5 to give a compound of Formula 1c.

In said Formulas 1c, 2c, 3c, 4c, and 5, Boc is an amine protecting group (e.g., tert-butoxycarbonyl, 9- fluorenylmethyloxycarbonyl (FMOC), benzyloxycarbonyl (CBZ), triphenylmethyl(trityl), etc.) and

R ₁ , R ₂ , R ₄ , and n are the same as defined in the above.

[0181] The compound of Formula 2c is commercially available. The reaction of the compound of Formula 2c above with R ₄ -NH2 can be carried out via amide coupling reaction. Said amide coupling reaction can be performed using a base such as diisopropylamine, diisopropylethylamine, triethylamine, etc., and a coupling reagent such as O-(7-azabenzotriazol-1-yl)-N,N,N',N'- tetramethylurinium hexafluorophosphate (HATU), O-(benzotriazol-1-yl)-N,N,N',N'- tetramethyluronium hexafluorophosphate (HBTU), etc. Said coupling reaction can be performed in organic solvents such as dichloromethane, N,N-dimethylformamide, etc., and can be carried out at room temperature or warm temperature conditions (20°C to 60°C).

[0182] Deprotection of the compound of Formula 3c can be carried out by conventional methods of removing an amine protecting group. For example, said amine protecting group in an organic solvent such as dichloromethane, etc. can be removed by using an acid such as trifluoroacetic acid or can be removed in the form of a hydrochloride salt by using hydrogen chloride dissolved in the organic solvents, such as diethyl ether, 1,4-dioxane, etc.

[0183] The reaction of the compound of Formula 4c above or a salt thereof with a compound of Formula 5 can be carried out via nucleophilic substitution. The nucleophilic substitution reaction can be carried out at room temperature or warm temperature conditions (20°C to 60°C) by using a base such as cesium carbonate, potassium carbonate, etc., in a solvent such as acetonitrile, dichloromethane, or the like.

[0184] Further, the present technology includes a preparation process of a compound of Formula 1d, wherein R ₃ in Formula 1 is -NHC(O)(CH ₂ ) _m R ₄ , or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof. That is, the present technology includes a preparation process of a compound of Formula 1d, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, comprising the step of reacting a compound of Formula 5 with a compound of Formula 9 to prepare a compound of Formula 10; the step of carrying out deprotection reaction of the compound of Formula 10 above to prepare a compound of Formula 11 or a salt thereof; and the step of reacting the compound of Formula 11 with R ₄ -(CH ₂ ) _m COOH to give a compound of Formula 1d.

In said Formulas 1d, 5, 9, 10, and 11, Boc is an amine protecting group (e.g., tert-butoxycarbonyl, 9- fluorenylmethyloxycarbonyl (FMOC), benzyloxycarbonyl (CBZ), triphenylmethyl(trityl), etc.) and

R ₁ , R ₂ , R ₄ , n, and m are the same as defined in the above.

[0185] The compound of Formula 9 is commercially available. The reaction of the compound of Formula 5 above with the compound of Formula 9 can be carried out via nucleophilic substitution. The nucleophilic substitution reaction can be carried out at room temperature or warm temperature conditions (20°C to 60°C) by using a base such as cesium carbonate, potassium carbonate, etc., in a solvent such as acetonitrile, dichloromethane, or the like.

[0186] Deprotection of the compound of Formula 10 can be carried out by conventional methods of removing an amine protecting group. For example, said amine protecting group in an organic solvent such as dichloromethane, etc. can be removed by using an acid such as trifluoroacetic acid or can be removed in the form of a hydrochloride salt by using hydrogen chloride dissolved in the organic solvents, such as diethyl ether, 1,4-dioxane, etc.

[0187] The reaction of the compound of Formula 11 above with R ₁ -(CH ₂ ) _m COOH can be carried out via amide coupling reaction. Said amide coupling reaction can be performed using a base such as diisopropylamine, diisopropylethylamine, triethylamine, etc., and a coupling reagent such as 0(7- azabenzotriazol-1-yl)-N,N,N',N'-tetramethylurinium hexafluorophosphate (HATU), O-(benzotriazol- 1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (HBTU), etc. Said coupling reaction can be performed in organic solvents such as dichloromethane, N,N-dimethylformamide, etc., and can be carried out at room temperature or warm temperature conditions (20°C to 60°C).

[0188] Further, the present technology includes a preparation process of a compound of Formula le, wherein R ₃ in Formula 1 is -NHS(O) ₂ R ₄ , or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof. That is, the present technology includes a preparation process of a compound of Formula le, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, comprising the step of reacting a compound of Formula 11 or a salt thereof with R ₄ -S(O) ₂ C1 to prepare a compound of Formula le.

In said Formulas le and 11, R ₁ , R ₂ , R ₄ , and n are the same as defined in the above.

[0189] The reaction of the compound of Formula 11 above or a salt thereof with R ₄ -S(O) ₂ C1 may be carried out via nucleophilic substitution. The nucleophilic substitution reaction can be carried out at room temperature or warm temperature conditions (20°C to 60°C) by using a base such as cesium carbonate, potassium carbonate, etc., in a solvent such as acetonitrile, dichloromethane, or the like.

[0190] Generally, FAP is not expressed in normal tissues but its expression increases in stromal fibroblasts of malignant tumors such as breast cancer, colorectal cancer, ovarian cancer, and the like (Garin-Chesa et al., Proc. Natl. Acad. Sci., 1990, 87: 7235). Accordingly, the inhibition of FAP can be a target of treatment of a malignant tumor. In fact, it was reported that Va1boroPro (PT-100, Talobostat) known as an FAP inhibitor, when orally administered, exhibits anticancer activities in mice by delaying the proliferation of tumors and inducing the degeneration of tumors (Adams et al., Cancer Res., 2004, 64:5471). However, Val-boroPro (PT-100, talabostat) known as an FAP inihibitor also has DPP4, PREP inhibitory activity, etc. Thus, the low selectivity for FAP needs to be improved (O. Ryabtsova et al. Bioorg. Med. Chem. Lett., 2012, 3412-3417).

[0191] In addition, since FAP regulates the metabolic functions and is expressed in activated hepatic stellate cells (aHSC) and tissue remodeling sites associated with liver fibrosis, the FAP inhibition may be useful for the treatment of nonalcoholic fatty liver disease (NAFLD). Nonalcoholic fatty liver disease (NAFLD), histologically, encompasses simple steatosis, nonalcoholic steatohepatitis (NASH), and liver cirrhosis. Among these, unlike simple steatosis (non-alcoholic fatty liver, NAFL), NASH may potentially progress to liver cirrhosis and hepatocellular carcinoma. In addition to insulin resistance, oxidative stress, inflammatory cascade, and fibrosis are known to play important roles in the progress of NASH. Thus, the inhibition of FAP may improve insulin resistance which is one of the key factors of NASH and may regulate the hepatoprotective effect and the function of activated hepatic stellate cells (aHSC) by FGF21 regulation and thereby may have the potential for treating NASH (Lay AJ et al., Front. Biosci. (Landmark Ed), 2019, 24, 1-17).

[0192] The pyrrolidine and piperidine compounds according to the present technology, i.e., the compound of Formulas X, Xa, Xb, 1, 1a, 1b, 1c, 1d, or 1e, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, have a inhibitory activity on FAP and thus can be usefully applied in the prevention or treatment of a disease mediated by fibroblast activation protein (FAP). Preferably, the compound of Formula 1 according to the present technology, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof can be usefully applied for the prevention or treatment of obesity, diabetes, steatosis, and nonalcoholic steatohepatitis (NASH).

[0193] In some embodiments, provided herein is the use of the pyrrolidine and piperidine compounds according to the present technology, i.e., the compound of Formulas X, Xa, Xb, 1, 1a, 1b, 1c, 1d, or 1e, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, in the preparation of a medicament for the prophylaxis and/or treatment of lipid and lipoprotein disorders (such as, but not limited to, hypercholesterolemia, hypertriglyceridemia, and atherosclerosis), of conditions and diseases which result from chronic fatty and fibrotic degeneration of organs due to accumulated lipid and specifically triglyceride accumulation and subsequent activation of profibrotic pathways (such as, but not limited to, NASH and chronic cholestatic conditions in the liver, Glomerulosclerosis and Diabetic Nephropathy in the kidney, Macular Degeneration and Diabetic Retinopathy in the eye and neurodegenerative diseases, such as Alzheimer's Disease in the brain, or Diabetic Neuropathies in the peripheral nervous system), of Type I or Type II Diabetes and clinical complications of Type I and Type II Diabetes (such as, but not limited to, Diabetic Nephropathy, Diabetic Retinopathy, Diabetic Neuropathies, or Peripheral Arterial Occlusive Disease (PAOD)), of chronic intrahepatic or some forms of extrahepatic cholestatic conditions, of liver fibrosis, of acute intraheptic cholestatic conditions, of obstructive or chronic inflammatory disorders that arise out of improper bile composition (such as, but not limited to, cholelithiasis also known as cholesterol gallstones), of gastrointestinal conditions with a reduced uptake of dietary fat and fat-soluble dietary vitamins, of inflammatory bowel diseases, of obesity and metabolic syndrome (combined conditions of dyslipidemia, diabetes and abnormally high body-mass index), of persistent infections by intracellular bacteria or parasitic protozoae, of non-malignant hyperproliferative disorders, of malignant hyperproliferative disorders (such as, but not limited to, different forms of cancer, specifically certain forms of breast, liver or colon cancer, or a disorder selected from the group consisting of hepatocellular carcinoma, colon adenoma, and polyposis), of colon adenocarcinoma and hepatocellular carcinoma in particular, of liver steatosis and associated syndromes, of Hepatitis B infection, of Hepatitis C infection and/or of cholestatic and fibrotic effects that are associated with alcohol-induced cirrhosis or with viral-bome forms of hepatitis, of liver failure or liver malfunction as an outcome of chronic liver diseases or of surgical liver resection, of acute myocardial infarction, of acute stroke, of thrombosis which occurs as an endpoint of chronic obstructive atherosclerosis, of osteoarthritis, of rheumatoid arthritis, of psoriasis, or of cerebral infarction, individually or of any combination thereof.

[0194] In some embodiments, the compounds and/or pharmaceutical compositions disclosed herein are used for prophylaxis and/or treatment of chronic intrahepatic conditions, such as Primary Biliary Cirrhosis (PBC), Primary Sclerosing Cholangitis (PSC), progressive familiar cholestasis (PFIC), alcohol-induced cirrhosis and associated cholestasis, and some forms of extrahepatic cholestatic conditions, or liver fibrosis.

[0195] In some embodiments, provided herein is a method to treat chronic intrahepatic conditions and/or some forms of extrahepatic cholestatic conditions in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein. In some embodiments, the chronic intrahepatic conditions are selected from PBC, PSC, PFIC, and alcohol-induced cirrhosis and associated cholestasis.

[0196] In some embodiments, provided herein is a method to treat liver fibrosis in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0197] In some embodiments, provided herein is a method to treat a lipid and lipoprotein disorder in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein. In some embodiments, the lipid and lipoprotein disorder is selected from hypercholesterolemia, hypertriglyceridemia, and atherosclerosis.

[0198] In some embodiments, provided herein is a method to treat a condition or disease which results from chronic fatty and fibrotic degeneration of organs due to accumulated lipid and specifically triglyceride accumulation and subsequent activation of profibrotic pathways in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein. In some embodiments, the condition or disease which results from chronic fatty and fibrotic degeneration of organs due to accumulated lipid and specifically triglyceride accumulation and subsequent activation of profibrotic pathways is selected from NASH and chronic cholestatic conditions in the liver, Glomerulosclerosis and Diabetic Nephropathy in the kidney, Macular Degeneration and Diabetic Retinopathy in the eye, and neurodegenerative diseases. In some further embodiments, neurodegenerative diseases are selected from Alzheimer's Disease in the brain, and Diabetic Neuropathies in the peripheral nervous system.

[0199] In some embodiments, provided herein is a method to treat Type I or Type II Diabetes and clinical complications of Type I and Type II Diabetes in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein. In some embodiments, provided herein is a method to treat Type I Diabetes in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein. In some embodiments, provided herein is a method to treat Type II Diabetes in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein. In some embodiments, provided herein is a method to treat one or more clinical complications of Type I and Type II Diabetes in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein. In some embodiments, the clinical complications of Type I and Type II Diabetes are selected from Diabetic Nephropathy, Diabetic Retinopathy, Diabetic Neuropathies, and Peripheral Arterial Occlusive Disease (PAOD), or any combination thereof.

[0200] In some embodiments, provided herein is a method to treat acute intraheptic cholestatic conditions in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0201] In some embodiments, provided herein is a method to treat obstructive or chronic inflammatory disorders that arise out of improper bile composition in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein. In some embodiments, the obstructive or chronic inflammatory disorders that arise out of improper bile composition is cholelithiasis also known as cholesterol gallstones.

[0202] In some embodiments, provided herein is a method to treat gastrointestinal conditions with a reduced uptake of dietary fat and fat-soluble dietary vitamins in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0203] In some embodiments, provided herein is a method to treat inflammatory bowel diseases in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0204] In some embodiments, provided herein is a method to treat obesity and metabolic syndrome in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0205] In some embodiments, provided herein is a method to treat persistent infections by intracellular bacteria or parasitic protozoae in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0206] In some embodiments, provided herein is a method to treat non-malignant hyperproliferative disorders in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0207] In some embodiments, provided herein is a method to treat malignant hyperproliferative disorders in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein. In some embodiments, malignant hyperproliferative disorders are selected from different forms of cancer, specifically certain forms of breast, liver or colon cancer, or a disorder selected from the group consisting of hepatocellular carcinoma, colon adenoma, and polyposis.

[0208] In some embodiments, provided herein is a method to treat colon adenocarcinoma in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0209] In some embodiments, provided herein is a method to treat hepatocellular carcinoma in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0210] In some embodiments, provided herein is a method to treat liver steatosis and associated syndromes in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein. [0211] In some embodiments, provided herein is a method to treat Hepatitis B infection in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0212] In some embodiments, provided herein is a method to treat Hepatitis C infection in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0213] In some embodiments, provided herein is a method to treat cholestatic and fibrotic effects that are associated with alcohol-induced cirrhosis or with viral-bome forms of hepatitis in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0214] In some embodiments, provided herein is a method to treat liver failure or liver malfunction as an outcome of chronic liver diseases or of surgical liver resection in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0215] In some embodiments, provided herein is a method to treat acute myocardial infarction in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0216] In some embodiments, provided herein is a method to treat acute stroke in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0217] In some embodiments, provided herein is a method to treat thrombosis which occurs as an endpoint of chronic obstructive atherosclerosis in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0218] In some embodiments, provided herein is a method to treat osteoarthritis in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0219] In some embodiments, provided herein is a method to treat rheumatoid arthritis in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0220] In some embodiments, provided herein is a method to treat psoriasis in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0221] In some embodiments, provided herein is a method to treat cerebral infarction in a patient in need thereof, the method comprising, consisting essentially of, or consisting of administering to the patient a therapeutically effective amount of a compound or a composition disclosed herein.

[0222] FAP is expressed not only in the form of being attached to a cell membrane but also in the form of being secreted from a cell and circulated in blood (circulating FAP, hereinafter, "c-FAP") and the two forms have the same enzymatic activity. c-FAP can be quantified at antigen levels using an enzyme-linked immunosorbent assay (ELISA) or can be quantified at enzymatic activity levels using an FAP-specific substrate, and this FAP quantitative analysis revealed that said levels correlate with the degree of induction of said diseases (Ultte de Willige S et al., PLoS One, June 5, 2017, 12). That is, c-FAP can be utilized as an indicator for diagnosis of a disease such as chronic liver disease, malignant tumor, etc .Accordingly, in another aspect, provided herein are methods of using the compounds disclosed herein with c-FAP detection. In some embodiments, provided herein are methods of diagnosing a disease described herein in a subject, the method comprising contacting blood from the subject with a compound described herein and ascertaining a concentration of c-FAP from the blood.

[0223] FGF21 is known to have anti-obesity and anti-diabetic effects (M. J. Potthoff et al., Nature

Reviews Endocrinology, 2017, 13, and 74), lipid metabolic regulation effect (Murata Y et al. J Nutr Metab. 2011; 2011: 981315), and is known to have a potent hepatoprotective effect (Y. Yu et al, Molecular & Cellular Biochemistry, 2015, 403, 287). Thus, the inhibition of FAP activity may regulate the degradation of FGF-21 by FAP and may be useful for the treatment of metabolic syndromes such as obesity, diabetes, etc.

[0224] Accordingly, in another aspect, provided herein are methods of regulating the degradation of FGF-21 by the inhibition of FAP using a compound described herein.

[0225] Thus, the present technology includes a pharmaceutical composition for inhibiting FAP, comprising a therapeutically effective amount of a compound of Formulas X, Xa, Xb, 1, 1a, 1b, 1c, 1d, or 1e, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof as an active ingredient. In one embodiment, the present technology provides a pharmaceutical composition for preventing or treating obesity, diabetes, steatosis, and nonalcoholic steatohepatitis

(NASH), comprising a therapeutically effective amount of a compound of Formulas X, Xa, Xb, 1, 1a, 1b, 1c, 1d, or 1e, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof as an active ingredient.

[0226] In some embodiments, the compounds of the present disclosure may be combined with one or more additional therapies for the prevention or treatment of a disease or condition amenable to treatment by inhibition of FAP.

[0227] In some embodiments, the compositions disclosed herein contain at least one additional active agent.

[0228] Exemplary additional active agents include, but are not limited to, one or more of a(n) ACE inhibitor, Acetyl CoA carboxylase inhibitor (e.g., firsocostat), Adenosine A3 receptor agonist, Adiponectin receptor agonist, AKT protein kinase inhibitor, AMP-activated protein kinases (AMPK), Amylin receptor agonist, Angiotensin II AT-1 receptor antagonist, Apoptosis Signaling Kinase 1 inhibitor, Autotaxin inhibitors, Bioactive lipid, Calcitonin agonist, Caspase inhibitor, Caspase-3 stimulator, Cathepsin inhibitor, Caveolin 1 inhibitor, CCR ₂ chemokine antagonist, CCR ₃ chemokine antagonist, CCR ₅ chemokine antagonist, CCR ₂ /CCR ₅ dual antagonist (e.g., cenicriviroc), Chloride channel stimulator, CNR1 inhibitor, Cyclin D1 inhibitor, Cytochrome P450 7A1 inhibitor, DGAT1/2 inhibitor, Dipeptidyl peptidase IV inhibitor, Endosialin modulator, Eotaxin ligand inhibitor, Extracellular matrix protein modulator, Famesoid X receptor agonist (e.g., obetacholic acid, tropofexor, or cilofexor), Fatty acid synthase inhibitors, FGF1 receptor agonists, Pegylated FGF21, Fibroblast growth factor (FGF-15, FGF-19, FGF-21) ligands, Galectin-3 inhibitor, Glucagon receptor agonist, Glucagon-like peptide 1 agonist, G-protein coupled bile acid receptor 1 agonist, Hedgehog (Hh) modulator, Hepatitis C virus NS3 protease inhibitor, Hepatocyte nuclear factor 4 alpha modulator (HNF4A), Hepatocyte growth factor modulator, HMG CoA reductase inhibitor, IL-10 agonist, IL-17 antagonist, Ileal sodium bile acid cotransporter inhibitor, Insulin sensitizer, integrin modulator, intereukin-1 receptor-associated kinase 4 (IRAK4) inhibitor, Jak2 tyrosine kinase inhibitor, ketohexokinase inhibitors, Klotho beta stimulator, 5 -Lipoxygenase inhibitor, Lipoprotein lipase inhibitor, Liver X receptor, LPL gene stimulator, Lysophosphatidate- 1 receptor antagonist, Lysyl oxidase homolog 2 inhibitor, Matrix metalloproteinase s (MMPs) inhibitor, MEKK-5 protein kinase inhibitor, Membrane copper amine oxidase (VAP-1) inhibitor, Methionine aminopeptidase-2 inhibitor, Methyl CpG binding protein 2 modulator, MicroRNA-21(miR-21) inhibitor, Mitochondrial uncoupler, Myelin basic protein stimulator, NACHT LRR PYD domain protein 3 (NLRP3) inhibitor, NAD- dependent deacetylase sirtuin stimulator, NADPH oxidase inhibitor (NOX), Nicotinic acid receptor 1 agonist, P2Y13 purinoceptor stimulator, PDE 3 inhibitor, PDE 4 inhibitor, PDE 5 inhibitor, PDGF receptor beta modulator, Phospholipase C inhibitor, PPAR alpha agonist, PPAR delta agonist, PPAR gamma agonist, PPAR gamma modulator (e.g., elafibrinor), Protease -activated receptor-2 antagonist, Protein kinase modulator, Rho associated protein kinase inhibitor, Sodium glucose transporter-2 inhibitor, SREBP transcription factor inhibitor, STAT-1 inhibitor, Stearoyl CoA desaturase-1 inhibitor, Suppressor of cytokine signalling- 1 stimulator, Suppressor of cytokine signalling-3 stimulator, Thyroid hormone receptor b-selective agonists such as MGL-3196, Transforming growth factor b (TGF-b), Transforming growth factor b activated Kinase 1 (TAK1), Thyroid hormone receptor beta agonist, TLR-4 antagonist, Transglutaminase inhibitor, Tyrosine kinase receptor modulator, GPCR modulator, nuclear hormone receptor modulator, WNT modulators, and YAP/TAZ modulator. Examples of JAK inhibitors include, but are not limited to, fdgotonib and tofacitinib. A non-limiting example of an apoptosis signal kinase inhibitor is selonsertib.

[0229] The compound of Formulas X, Xa, Xb, 1, 1a, 1b, 1c, 1d, or 1e, or the stereoisomer or mixture of stereoisomers thereof, or the pharmaceutically acceptable salt thereof, and at least one additional active agent may be administered in any order or even simultaneously. The multiple active agents may be provided in a single, unified form, or in multiple forms (by way of example only, either as a single pill or as two separate pills). One of the active agents may be given in multiple doses, or both may be given as multiple doses. If not simultaneous, the timing between the multiple doses may vary from more than zero weeks to less than four weeks. In addition, the combination methods, compositions and formulations are not to be limited to the use of only two agents.

[0230] The pharmaceutical composition of the present technology may comprise a pharmaceutically acceptable carrier, such as diluents, disintegrants, sweetening agents, glidants, or flavoring agents and may be formulated into an oral dosage form such as tablets, capsules, powders, granules, suspensions, emulsions, or syrups; or a parenteral dosage form such as liquids for external use, suspensions for external use, emulsions for external use, gels (ointments or the like), inhaling agents, spraying agents, injections, etc. Said dosage forms may be formulated in various forms, e.g., a dosage form for single administration or for multiple administrations.

[0231] The pharmaceutical composition of the present technology may comprise excipients such as lactose, com starch, or the like, glidants such as magnesium stearate, etc., emulsifying agents, suspending agents, stabilizers, and isotonic agents, etc. If desired, a sweetening agent and/or a flavoring agent may be added. Exemplary excipients include, without limitation, polyethylene glycol (PEG), hydrogenated castor oil (HCO), cremophors, carbohydrates, starches (e.g., com starch), inorganic salts, antimicrobial agents, antioxidants, binders/fillers, surfactants, lubricants (e.g., calcium or magnesium stearate), glidants such as talc, disintegrants, diluents, buffers, acids, bases, film coats, combinations thereof, and the like.

[0232] Specific carbohydrate excipients include, for example: monosaccharides, such as fructose, maltose, galactose, glucose, D-mannose, sorbose, and the like; disaccharides, such as lactose, sucrose, trehalose, cellobiose, and the like; polysaccharides, such as raffmose, melezitose, maltodextrins, dextrans, starches, and the like; and alditols, such as mannitol, xylitol, maltitol, lactitol, xylitol, sorbitol (glucitol), pyranosyl sorbitol, myoinositol, and the like.

[0233] Inorganic salt or buffers include, but are not limited to, citric acid, sodium chloride, potassium chloride, sodium sulfate, potassium nitrate, sodium phosphate monobasic, sodium phosphate dibasic, and combinations thereof.

[0234] Suitable antioxidants for use in the present disclosure include, for example, ascorbyl palmitate, butylated hydroxyanisole, butylated hydroxytoluene, hypophosphorous acid, monothioglycerol, propyl gallate, sodium bisulfite, sodium formaldehyde sulfoxylate, sodium metabisulfite, and combinations thereof.

[0235] Additional exemplary excipients include surfactants such as polysorbates, e.g., “Tween 20” and “Tween 80,” and pluronics such as F68 and F88 (both of which are available from BASF, Mount Olive, N.J.), sorbitan esters, lipids (e.g., phospholipids such as lecithin and other phosphatidy1cholines, and phosphatidylethanolamines), fatty acids and fatty esters, steroids such as cholesterol, and chelating agents, such as EDTA, zinc and other such suitable cations.

[0236] Further, a composition disclosed herein may optionally include one or more acids or bases. Non-limiting examples of acids that can be used include those acids selected from the group consisting of hydrochloric acid, acetic acid, phosphoric acid, citric acid, malic acid, lactic acid, formic acid, trichloroacetic acid, nitric acid, perchloric acid, phosphoric acid, sulfuric acid, fumaric acid, and combinations thereof. Non-limiting examples of suitable bases include bases selected from the group consisting of sodium hydroxide, sodium acetate, ammonium hydroxide, potassium hydroxide, ammonium acetate, potassium acetate, sodium phosphate, potassium phosphate, sodium citrate, sodium formate, sodium sulfate, potassium sulfate, potassium fumerate, and combinations thereof.

[0237] The amount of any individual excipient in the composition will vary depending on the role of the excipient, the dosage requirements of the active agent components, and particular needs of the composition.

[0238] Generally, however, the excipient will be present in the composition in an amount of about 1% to about 99% by weight, preferably from about 5% to about 98% by weight, more preferably from about 15 to about 95% by weight of the excipient. In general, the amount of excipient present in a composition of the disclosure is selected from the following: at least about 2%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or even 95% by weight.

[0239] The composition of the present technology can be administered orally or parenterally, including inhalation, intravenous, intraperitoneal, subcutaneous, rectal and topical administration routes. Therefore, the composition of the present technology can be formulated into various forms such as tablets, capsules, aqueous solutions, suspensions, or the like. In the case of tablets for oral administration, carriers such as lactose, com starch, and lubricating agents, e.g. magnesium stearate, can be conventionally added thereto. In the case of capsules for oral administration, lactose and/or dried com starch can be used as a diluent. When an aqueous suspension is required for oral administration, the active ingredient can be combined with emulsifying and/or suspending agents. If desired, certain sweetening agents and/or flavoring agents can be added thereto. For intramuscular, intraperitoneal, subcutaneous and intravenous administration, sterile solutions of the active ingredient are usually prepared, and the pH of the solutions should be suitably adjusted and buffered. For intravenous administration, the total concentration of solutes should be controlled in order to render the preparation isotonic. The composition of the present technology may be in the form of an aqueous solution containing pharmaceutically acceptable carriers, e.g., saline having a pH level of 7.4. The solutions may be introduced into a patient's intramuscular blood-stream by local bolus injection.

[0240] Said pyrrolidine or piperidine compound, i.e., the compound of Formulas X, Xa, Xb, 1, 1a, 1b, 1c, 1d, or 1e, or its stereoisomer or mixture of stereoisomers, or its pharmaceutically acceptable salt, can be administered to a patient in an effective amount ranging from about 0.001 mg/kg to about 100 mg/kg per day. Of course, the dosage may be changed according to the patient's age, weight, susceptibility, symptom, or the efficacy of the compound. This includes 0.001, 0.0025,

0.005, 0.0075, 0.01, 0.025, 0.05, 0.075, 0.1, 0.25, 0.5, 0.75, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30,

35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or 100 mg/kg.

[0241] Generally, a therapeutically effective amount of the compound of Formulas X, Xa, Xb, 1, 1a, 1b, 1c, 1d, or 1e, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, will range from a total daily dosage of about 0.1 mg/day to 1000 mg/day, about 30-720 mg/day, about 60-600 mg/day, or about 100-480 mg/day, or more. In some embodiments, a therapeutically effective amount of the compound of Formulas X, Xa, Xb, 1, 1a, 1b, 1c, 1d, or 1e, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, will range from about 1-240 mg/day, about 30-240 mg/day, about 30-200 mg/day, about 30-120 mg/day, about 1-120 mg/day, about 50-150 mg/day, about 60-150 mg/day, about 60-120 mg/day, or about 60-100 mg/day, administered as either a single dosage or as multiple dosages. In some embodiments, multiple dosages include two, three, or four doses per day.

[0242] In some embodiments, the therapeutically effective amount of the compound of Formulas

X, Xa, Xb, 1, 1a, 1b, 1c, 1d, or 1e, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof, is at least 0.1 mg/day, at least 0.5 mg/day, at least 1 mg/day, at least 5 mg/day, at least 10 mg/day, at least 20 mg/day, at least 30 mg/day, at least 40 mg/day, at least

50 mg/day, at least 60 mg/day, at least 70 mg/day, at least 80 mg/day, at least 90 mg/day, at least 100 mg/day, at least 110 mg/day, at least 120 mg/day, at least 130 mg/day, at least 140 mg/day, at least 150 mg/day, at least 160 mg/day, at least 170 mg/day, at least 180 mg/day, at least 190 mg/day, at least 200 mg/day, at least 225 mg/day, at least 250 mg/day, at least 275 mg/day, at least 300 mg/day, at least 325 mg/day, at least 350 mg/day, at least 375 mg/day, at least 400 mg/day, at least 425 mg/day, at least 450 mg/day, at least 475 mg/day, at least 500 mg/day, at least 525 mg/day, at least 550 mg/day, at least 575 mg/day, at least 600 mg/day, at least 625 mg/day, at least 650 mg/day, at least 675 mg/day, at least 700 mg/day, at least 725 mg/day, at least 750 mg/day, at least 775 mg/day, at least 800 mg/day, at least 825 mg/day, at least 850 mg/day, at least 875 mg/day, at least 900 mg/day, at least 925 mg/day, at least 950 mg/day, at least 975 mg/day, or at least 1000 mg/day.

[0243] The present technology also includes a method of inhibiting a fibroblast activation protein (FAP) in a mammal, comprising administering to the mammal a therapeutically effective amount of the compound of Formulas X, Xa, Xb, 1, 1a, 1b, 1c, 1d, or 1e, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof. In one embodiment, the present technology provides a method for treating obesity, diabetes, steatosis, and nonalcoholic steatohepatitis (NASH), comprising administering, to a mammal, a therapeutically effective amount of the compound of Formulas X, Xa, Xb, 1, 1a, 1b, 1c, 1d, or 1e, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof. In some embodiments, provided herein is a method for treating NASH in a subject in need thereof, the method comprising, consisting essentially of, or consisting of administering to the subject a therapeutically effective amount of a compound of Formulas X, Xa, Xb, 1, 1a, 1b, 1c, 1d, or 1e, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof. Mammals include, but are not limited to, mice, rodents, rats, simians, humans, farm animals, dogs, cats, sport animals, and pets.

[0244] The present technology provides a use of the compound of Formulas X, Xa, Xb, 1, 1a, 1b, 1c, 1d, or 1e, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof for use in the manufacture of a medicament for inhibiting a fibroblast activation protein (FAP) in mammals. In one embodiment, the present technology provides a use of the compound of Formulas X, Xa, Xb, 1, 1a, 1b, 1c, 1d, or 1e, or a stereoisomer or mixture of stereoisomers thereof, or a pharmaceutically acceptable salt thereof for use in the manufacture of a medicament for treating or preventing obesity, diabetes, steatosis, and nonalcoholic steatohepatitis (NASH).

[0245] Hereinafter, the present technology is further elaborated through Examples and Experimental Examples. However, the following examples and experimental examples are provided for illustration purposes only, and are not intended to limit the scope of the invention.

[0246] As will be apparent to those of skill in the art upon reading this disclosure, each of the individual embodiments described and illustrated herein has discrete components and features which may be readily separated from or combined with the features of any of the other several embodiments without departing from the scope or spirit of the present technology. Any recited method can be carried out in the order of events recited or in any other order which is logically possible.

EXAMPLES

[0247] The analyses of the compounds prepared in the following examples were carried out as follows: Nuclear magnetic resonance (NMR) spectrum analysis was carried out using Bruker 400 MHz spectrometer and Agilent 600 MHz spectrometer and chemical shifts thereof were analyzed in ppm. Further, the indicated molecular weights were measured by using liquid chromatography/mass selective detector (MSD) of Agilent 1260 Infinity series equipped with an electrostatic spray interface (by using Single Quadrupole, it indicates a value of m/z in ESI+ (ESI-MS (cation), which is represented by the [M + H] + peak). Column chromatography was carried out on silica gel (Merck, 70-230 mesh). (W.C. Still, J. Org. Chem., 43, 2923, 1978). Further, the abbreviations used in the following examples are as follows: methyl is abbreviated to Me, ethyl is abbreviated to Et, phenyl is abbreviated to Ph, and tert-butyloxycarbonyl is abbreviated to Boc. Further, the starting materials in each [0248] [0249] Example are known compounds, which were synthesized according to literatures or obtained from the market such as Sigma- Aldrich.

[0250] Reference Example 1. (S)-1-(2-chloroacetyl)pyrrolidine-2-carbonitrile

[0251] (S)-Pyrrolidine-2-carbonitrile hydrochloride (7 g, 52.8 mmol) was dissolved in dichloromethane (264 mL). The resulting solution was dropwise added with triethylamine (22.1 mL, 158.3 mmol) and then cooled to 0°C. The solution in which 2-chloroacety1chloride (4.63 mL, 58.1 mmol) was dissolved in dichloromethane (10 mL) was slowly added dropwise to the reaction mixture. The reaction mixture was stirred for 2 hours at 0°C and then added with water to extract an aqueous layer with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 2/1, v/v) to give the title compound (7.6 g). ¹ H-NMR (CDCI ₃ , 400MHz) δ 4.81 (d, 1H), 4.24-4.11 (m, 2H), 3.73 (s, 2H), 3.61 (s, 1H), 3.61 (s, 1H) 2.42-2.05 (m, 4H)

[0252] Reference Example 2. (2S,4S)-1-(2-chloroacetyl)-4-fluoro-pyrrolidine-2-carbonitri le

[0253] The title compound as a solid (6.31 g) was prepared in the same fashion as Reference Example 1 except that (2S,4S)-4-fluoro-pyrrolidine-2-carbonitrile hydrochloride (7.95 g, 52.8 mmol) was used instead of (S)-pyrrolidine-2-carbonitrile hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 5.52-5.31 (m, 1H), 5.06-4.94 (m, 1H), 4.26-3.84 (m, 3H) 2.80-2.68 (m, 1H), 2.42-2.32 (m, 1H)

[0254] Reference Example 3. (2S)-1-(2-chloroacetyl)-4,4-difluoro-pyrrolidine-2-carbonitr ile

[0255] The title compound as a solid (8.57 g) was prepared in the same fashion as Reference Example 1 except that (2S)-4,4-difluoropyrrolidine-2-carbonitrile 4-methylbenzenesulfonate (16.1 g, 52.8 mmol) was used instead of (S)-pyrrolidine-2-carbonitrile hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 4.97 (s, 1H), 4.04 (s, 3H), 2.79 (s, 2H), 1.55 (s, 1H)

[0256] Reference Example 4. (1S,3S,5S)-2-(2-chloroacetyl)-2-azabicyclo[3.1.0]hexane-3- carbonitrile

[0257] The title compound as a liquid (8 g) was prepared in the same fashion as Reference Example 1 except that (1S,3S,5S)-2-azabicyclo[3.1.0]hexane-3-carbonitrile 4-methylbenzenesulfonate (14.8 g, 52.8 mmol) was used instead of (S)-pyrrolidine-2-carbonitrile hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 4.96 (d, 1H), 4.25-4.14 (m, 2H), 3.62-3.58 (m, 1H), 2.65-2.58 (m, 1H), 2.42 (d, 1H), 1.94-1.90 (m, 1H), 1.16-1.05 (m, 2H)

[0258] Reference Example 5. (S)-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0259] Step 1: tert-butyl (S)-3-(quinolin-4-ylamino)pyrrolidine-1-carboxylate

[0260] Tert-butyl (S)-3-aminopyrrolidine-1-carboxylate (500 mg, 2.68 mmol) was dissolved in anhydrous toluene. To the resulting solution, 4-bromoquinoline (670.2 mg, 3.22 mmol), cesium carbonate (1.31 g, 4.02 mmol), tris(dibenzylideneacetone)dipalladium(0) (245.8 mg, 0.27 mmol), and (+/-)-2,2'-bis(diphenylphospino)- 1,1'-binaphthalene (167. lmg, 0.27 mmol) were added. The reaction mixture was refluxed and stirred at 100°C overnight. After the completion of the reaction, the reaction mixture was cooled to room temperature and then added with water to extract an aqueous layer with ethyl acetate. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 1/1, v/v) to give the title compound (633.5 mg). ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.53 (d, 1H), 8.14 (d, 1H), 7.99 (d, 1H), 7.60 (t, 1H), 7.36 (t, 1H), 6.47 (d, 1H), 4.80 (m, 1H), 4.39 (m, 1H), 3.89-3.98 (m, 1H), 3.79-3.89 (m, 1H), 3.63-3.74 (m, 1H), 3.51-3.63 (m, 1H), 2.21-2.30 (m, 1H), 1.96-2.08 (m, 1H), 1.46 (s, 9H)

[0261] Step 2: (S)-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0262] Tert-butyl (S)-3-(quinolin-4-ylamino)pyrrolidine-1-carboxylate (472 mg, 1.51 mmol) prepared in Step 1 was dissolved in dichloromethane (8.4 ml) and then 4.0 M of hydrochloric acid dissolved in 1,4-dioxane was added thereto in excess drops. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, the solid compound obtained therefrom was filtered, washed, and vacuum dried to prepare the title compound (394.5 mg). ¹ H- NMR ((CD ₃ ) ₂ SO, 400MHz) δ 14.6 (s, 1H), 9.75 (s, 1H), 9.46 (d, 1H), 8.98 (d, 1H), 8.66 (d, 1H), 8.08 (d, 1H), 8.00 (dd, 1H), 7.74 (dd, 1H), 6.98 (d, 1H), 4.74-4.82 (m, 1H), 3.35-3.59 (m, 2H), 2.23-2.32 (m, 1H), 2.18-2.28 (m, 1H) [0263] Reference Example 6. (S)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride

[0264] Step 1: tert-butyl (S)-3-(quinolin-3-ylamino)pyrrolidine-1-carboxylate

[0265] The title compound (576.3 mg) was prepared in the same fashion as Step 1 of Reference Example 5, except that 3-bromoquinoline (670.2 mg, 3.22 mmol) was used instead of 4- bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.40-8.53 (m, 1H), 7.88-8.02 (m, 1H), 7.60-7.74 (m, 1H), 7.34-7.55 (m, 2H), 6.95-7.09 (m, 1H), 4.17-4.33 (m, 1H), 3.80 (m, 1H), 3.42-3.64 (m, 2H), 3.21- 3.41 (m, 1H), 2.13-2.42 (m, 2H), 1.87-2.02 (m, 1H), 1.34-1.60 (m, 9H)

[0266] Step 2: (S)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride

[0267] The title compound (473.5 mg) was prepared in the same fashion as Step 2 of Reference Example 5 and used in the next step without any further purification.

[0268] Reference Example 7. (S)-N-(pyrrolidin-3-yl)isoquinolin-4-amine hydrochloride

[0269] Step 1: tert-butyl (S)-3-(isoquinolin-4-ylamino)pyrrolidine-1-carboxylate

[0270] The title compound (457.9 mg) was prepared in the same fashion as Step 1 of Reference Example 5, except that 4-bromoisoquinoline (670.2 mg, 3.22 mmol) was used instead of 4- bromoquinoline. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.85 (s, 1H), 8.11 (d, 1H), 8.01-8.15 (m, 1H), 7.93 (d, 1H), 7.54-7.68 (m, 2H), 4.91-4.94 (m, 1H), 4.39-4.48 (m, 1H), 3.62-3.86 (m, 2H), 3.29-3.43 (m, 2H), 2.31-2.41 (m, 1H), 1.90-1.99 (m, 1H), 1.47 (s, 9H)

[0271] Step 2: (S)-N-(pyrrolidin-3-yl)isoquinolin-4-amine hydrochloride

[0272] The title compound (343.2 mg) was prepared in the same fashion as Step 2 of Reference Example 5 and used in the next step without any further purification.

[0273] Reference Example 8. (S)-N-(benzo[b]thiophen-4-yl)pyrrolidin-3-amine hydrochloride

[0274] Step 1: tert-butyl (S)-3-(benzo[b]thiophen-4-ylamino)pyrrolidine-1-carboxylate

[0275] The title compound (351.6 mg) was prepared in the same fashion as Step 1 of Reference Example 5, except that 4-bromobenzo[b]thiophene (686.2 mg, 3.22 mmol) was used instead of 4- bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.26-7.37 (m, 2H), 7.15-7.24 (m, 2H), 6.53 (d, 1H), 4.07-4.25 (m, 2H), 3.77-3.82 (m, 1H), 3.43-3.63 (m, 2H), 3.26-3.42 (m, 1H), 2.25-2.35 (m, 1H), 1.93- 2.07 (m, 1H), 1.42-1.52 (m, 9H)

[0276] Step 2: (S)-N-(benzo[b]thiophen-4-yl)pyrrolidin-3-amine hydrochloride

[0277] The title compound (200.3 mg) was prepared in the same fashion as Step 2 of Reference Example 5 and used in the next step without any further purification.

[0278] Reference Example 9. (S)-N-(benzo[b]thiophen-7-yl)pyrrolidin-3-amine hydrochloride [0279] Step 1: tert-butyl (S)-3-(benzo[b]thiophen-7-ylamino)pyrrolidine-1-carboxylate

[0280] The title compound (264.5 mg) was prepared in the same fashion as Step 1 of Reference Example 5, except that 7-bromobenzo[b]thiophene (686.2 mg, 3.22 mmol) was used instead of 4- bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.34-7.40 (m, 1H), 7.26-7.33 (m, 3H), 6.59 (d, 1H), 4.23-4.32 (m, 1H), 3.79-3.88 (m, 1H), 3.70-3.82 (m, 1H), 3.46-3.61 (m, 2H), 3.24-3.42 (m, 1H), 2.24- 2.32 (m, 1H), 1.90-2.04 (m, 1H), 1.47 (s, 9H)

[0281] Step 2: (S)-N-(benzo[b]thiophen-7-yl)pyrrolidin-3-amine hydrochloride

[0282] The title compound (172 mg) was prepared in the same fashion as Step 2 of Reference Example 5 and used in the next step without any further purification.

[0283] Reference Example 10. (S)-N-(benzofuran-7-yl)pyrrolidin-3-amine hydrochloride

[0284] Step 1: tert-butyl (S)-3-(benzofuran-7-ylamino)pyrrolidine-1-carboxylate

[0285] The title compound (555.9mg) was prepared in the same fashion as Step 1 of Reference Example 5, except that 7-bromobenzofuran (641.2 mg, 3.22 mmol) was used instead of 4- bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.55-7.67 (m, 1H), 7.10-7.35 (m, 1H), 6.99-7.05 (m, 1H), 6.72-6.85 (m, 1H), 6.54 (d, 1H), 4.19-4.31 (m, 2H), 3.69-3.82 (m, 1H), 3.48-3.62 (m, 2H), 3.27- 3.43 (m, 1H), 2.18-2.27 (m, 1H), 1.97-2.03 (m, 1H), 1.46 (s, 9H)

[0286] Step 2: (S)-N-(benzofuran-7-yl)pyrrolidin-3-amine hydrochloride

[0287] The title compound (342.8 mg) was prepared in the same fashion as Step 2 of [0288]

Reference Example 5 and used in the next step without any further purification.

[0289] Reference Example 11. (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7-amine hydrochloride

[0290] Step 1: tert-butyl (S)-3-(furo[3,2-c]pyridin-7-ylamino)pyrrolidine-1-carboxylat e

[0291] The title compound (171.5 mg) was prepared in the same fashion as Step 1 of Reference

Example 5, except that 7-bromofuro[3,2-c]pyridine (640.2 mg, 3.22 mmol) was used instead of 4- bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.40 (s, 1H), 7.91 (s, 1H), 7.60 (d, 1H), 6.81 (d, 1H), 4.30-4.45 (m, 1H), 4.07-4.29 (m, 1H), 3.76-3.89 (m, 1H), 3.47-3.60 (m, 2H), 3.28-3.44 (m, 1H), 2.43- 2.65 (m, 1H), 2.19-2.34 (m, 11H), 1.94-2.03 (m, 1H), 1.46 (s, 9H)

[0292] Step 2: (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7-amine hydrochloride

[0293] The title compound (100.9 mg) was prepared in the same fashion as Step 2 of Reference

Example 5 and used in the next step without any further purification.

[0294] Reference Example 12. (S)-N-([1,1'-biphenyl]-2-yl)pyrrolidin-3-amine hydrochloride

[0295] Step 1: tert-butyl (S)-3-([1,1'-biphenyl]-2-ylamino)pyrrolidine-1-carboxylate [0296] The title compound (836.3 mg) was prepared in the same fashion as Step 1 of Reference Example 5, except that 2-bromobiphenyl (750.0 mg, 3.22 mmol) was used instead of 4- bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.40-7.58 (m, 2H), 7.32-7.40 (m, 3H), 7.18-7.24 (m, 1H), 7.04-7.13 (m, 1H), 6.75-6.86 (m, 1H), 6.66-6.72 (m, 1H), 4.03-4.10 (m, 1H), 3.93-4.01 (m, 1H), 3.67-3.75 (m, 1H), 3.27-3.47 (m, 2H), 3.11-3.25 (m, 1H), 2.09-2.19 (m, 1H), 1.48-1.58 (m, 1H), 1.38- 1.47 (s, 9H)

[0297] Step 2: (S)-N-([1,1'-biphenyl]-2-yl)pyrrolidin-3-amine hydrochloride

[0298] The title compound (562 mg) was prepared in the same fashion as Step 2 of Reference Example 5 and used in the next step without any further purification.

[0299] Reference Example 13. (S)-N-([1,1'-biphenyl]-4-yl)pyrrolidin-3-amine hydrochloride

[0300] Step 1: tert-butyl (S)-3-([1,1'-biphenyl]-4-ylamino)pyrrolidine-1-carboxylate

[0301] The title compound (756.5 mg) was prepared in the same fashion as Step 1 of Reference Example 5, except that 4-bromobiphenyl (750.0 mg, 3.22 mmol) was used instead of 4- bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.47-7.56 (m, 2H), 7.40-7.46 (m, 2H), 7.35-7.40 (m, 2H), 7.22-7.29 (m, 2H), 6.66-6.68 (m, 1H), 3.99-4.08 (m, 1H), 3.87-3.91 (m, 1H), 3.70-3.85 (m, 1H), 3.42-3.54 (m, 2H), 3.18-3.32 (m, 1H), 2.13-2.21 (m, 1H), 1.42-1.51 (m, 9H)

[0302] Step 2: (S)-N-([1,1'-biphenyl]-4-yl)pyrrolidin-3-amine hydrochloride

[0303] The title compound (414 mg) was prepared in the same fashion as Step 2 of Reference Example 5 and used in the next step without any further purification.

[0304] Reference Example 14. (S)-N-(naphthalen-2-yl)pyrrolidin-3-amine

[0305] Step 1: tert-butyl (S)-3-(naphthalen-2-ylamino)pyrrolidine-1-carboxylate

[0306] Tert-butyl (S)-3-aminopyrrolidine-1-carboxylate (500 mg, 2.68 mmol) was dissolved in anhydrous toluene. To the resulting solution, 2-bromonaphthalene (665.2 mg, 3.22 mmol), cesium carbonate (1.31 g, 4.02 mmol), tris(dibenzylideneacetone)dipalladium(0) (245.8 mg, 0.27 mmol), and (+/-)-2,2'-bis(diphenylphospino)-1,1'-binaphthalene (167. lmg, 0.27 mmol) were added. The reaction mixture was refluxed and stirred at 100°C overnight. After the completion of the reaction, the reaction mixture was cooled to room temperature and then added with water to extract an aqueous layer with ethyl acetate. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 1/1, v/v) to give the title compound (633.5 mg). MS (ESI) m/z= 312.4 (M + H)+

[0307] Step 2: (S)-N-(naphthalen-2-yl)pyrrolidin-3-amine

[0308] Tert-butyl (S)-3-(naphthalen-2-ylamino)pyrrolidine-1-carboxylate (633.5 mg, 2.02 mmol) prepared in Step 1 was dissolved in dichloromethane (11.2 ml) and then 4.0 M hydrochloric acid dissolved in 1,4-dioxane was added thereto in excess drops. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, the residue obtained by concentration under reduced pressure was dissolved in dichloromethane, and the solution was washed with an IN sodium hydroxide aqueous solution and brine, dried over anhydrous magnesium sulfate, and then concentrated under reduced pressure to produce the title compound (318.6 mg). ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.58-7.71 (m, 3H), 7.36 (dd, 1H), 7.19 (dd, 1H), 6.84 (dd, 1H), 6.74-6.82 (m,

1H), 4.01-4.12 (m, 1H), 3.88-3.98 (m, 0.9H), 3.73-3.79 (m, 0.2H), 3.57-3.65 (m, 0.3H), 3.04-3.30 (m, 2H), 2.81-3.00 (m, 2H), 2.16-2.28 (m, 1H), 1.64-1.78 (m, 1H)

[0309] Reference Example 15. (S)-N-([1,1'-biphenyl]-3-yl)pyrrolidin-3-amine hydrochloride

[0310] Step 1: tert-butyl (S)-3-([1,1'-biphenyl]-3-ylamino)pyrrolidine-1-carboxylate

[0311] The title compound (562.4 mg) was prepared in the same fashion as Step 1 of Reference Example 5, except that 3-bromobiphenyl (750.0 mg, 3.22 mmol) was used instead of 4- bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.52-7.60 (m, 2H), 7.36-7.47 (m, 3H), 7.31-7.35 (m, 1H), 6.96-7.05 (m, 1H), 6.80-6.93 (m, 1H), 6.60-6.75 (m, 1H), 4.03-4.15 (m, 1H), 3.84-3.95 (m, 1H), 3.65-3.77 (m, 1H), 3.47-3.52 (m, 1H), 3.18-3.35 (m, 1H), 2.15-2.25 (m, 1H), 1.86-1.98 (m, 1H), 1.40- 1.52 (m, 9H)

[0312] Step 2: (S)-N-([1,1'-biphenyl]-3-yl)pyrrolidin-3-amine hydrochloride

[0313] The title compound (342.2 mg) was prepared in the same fashion as Step 2 of Reference Example 5 and used in the next step without any further purification.

[0314] Reference Example 16. (S)-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0315] Step 1: tert-butyl (S)-3-(quinolin-5-ylamino)pyrrolidine-1-carboxylate

[0316] The title compound (297.4 mg) was prepared in the same fashion as Step 1 of Reference Example 5, except that 5-bromoquinoline (670.2 mg, 3.22 mmol) was used instead of 4- bromoquinoline. ¹ H-NMR (CD ₃ OD, 400MHz) δ 9.67-9.72 (m, 1H), 9.06-9.21 (m, 1H), 7.90-8.00 (m, 2H), 7.46-7.52 (m, 1H), 7.01-7.11 (m, 1H), 4.58 (m, 1H), 3.60-3.75 (m, 2H), 3.53-3.59 (m, 2H), 2.45-2.52 (m, 1H), 2.37-2.42 (m, 1H)

[0317] Step 2: (S)-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0318] The title compound (342.2 mg) was prepared in the same fashion as Step 2 of Reference Example 5 and used in the next step without any further purification.

[0319] Reference Example 17. (R)-N-(pyrrolidin-3-yl)quinolin-3 -amine hydrochloride

[0320] Step 1: tert-butyl (R)-3-(quinolin-3-ylamino)pyrrolidine-1-carboxylate [0321] Tert-butyl (R)-3-aminopyrrolidine-1-carboxylate (500 mg, 2.68 mmol) was dissolved in anhydrous toluene. To the resulting solution, 3-bromoquinoline (670.2 mg, 3.22 mmol), cesium carbonate (1.31 g, 4.02 mmol), tris(dibenzylideneacetone)dipalladium(0) (245.8 mg, 0.27 mmol), and (+/-)-2,2'-bis(diphenylphospino)-1,1'-binaphthalene (167. lmg, 0.27 mmol) were added. The reaction mixture was refluxed and stirred at 100°C overnight. After the completion of the reaction, the reaction mixture was cooled to room temperature and then added with water to extract an aqueous layer with ethyl acetate. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 1/1, v/v) to give the title compound (472 mg). ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.41-8.52 (m, 1H), 7.94-8.11 (m, 1H), 7.57-7.68 (m, 1H), 7.38-7.51 (m, 2H), 7.02-7.13 (m, 1H), 4.27 (m, 1H), 3.89-3.92 (m, 1H), 3.51-3.75 (m, 2H), 3.32-3.42 (m, 1H), 2.26-2.31 (m, 1H), 1.90-2.13 (m, 2H), 1.40-1.60 (m, 9H)

[0322] Step 2: (R)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride

[0323] The title compound (344.7 mg) was prepared in the same fashion as Step 2 of Reference Example 5 and used in the next step without any further purification.

[0324] Reference Example 18. (R)-N-([1,1'-biphenyl]-3-yl)pyrrolidin-3-amine hydrochloride

[0325] Step 1 : tert-butyl (R)-3 -([1,1 '-biphenyl] -3 -yl)pyrrolidine- 1 -carboxylate

[0326] The title compound (621.3 mg) was prepared in the same fashion as Step 1 of Reference Example 17, except that 3-bromobiphenyl (750.0 mg, 3.22 mmol) was used instead of 3- bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.55-7.68 (m, 2H), 7.38-7.53 (m, 2H), 7.30-7.35 (m, 1H), 7.23-7.28 (m, 1H), 6.94-7.08 (m, 1H), 6.79-6.84 (m, 1H), 6.58-6.63 (m, 1H), 4.04-4.12 (m, 1H), 3.90-4.03 (m, 1H), 3.71-3.85 (m, 1H), 3.46-3.52 (m, 2H), 3.15-3.35 (m, 1H), 2.16-2.20 (m, 1H), 1.82- 1.98 (m, 1H), 1.46-1.58 (m, 9H)

[0327] Step 2: (R)-N-([1,1'-biphenyl]-3-yl)pyrrolidin-3-amine hydrochloride

[0328] The title compound (383.7 mg) was prepared in the same fashion as Step 2 of Reference Example 5 and used in the next step without any further purification.

[0329] Reference Example 19. (R)-N-(pyrrolidin-3-yl)isoquinolin-4-amine hydrochloride

[0330] Step 1: tert-butyl (R)-3-(isoquinolin-4-ylamino)pyrrolidine-1-carboxylate

[0331] The title compound (447.7 mg) was prepared in the same fashion as Step 1 of Reference Example 17, except that 4-bromoisoquinoline (670.2 mg, 3.22 mmol) was used instead of 3- bromoquinoline. MS (ESI) m/z= 314.2 (M + H)+

[0332] Step 2: (R)-N-(pyrrolidin-3-yl)isoquinolin-4-amine hydrochloride [0333] The title compound (299.7 mg) was prepared in the same fashion as Step 2 of Reference Example 5. MS (ESI) m/z= 214.2 (M + H)+

[0334] Reference Example 20. (R)-N-(benzo[b]thiophen-7-yl)pyrrolidin-3-amine hydrochloride

[0335] Step 1: tert-butyl (R)-3-(benzo[b]thiophen-7-ylamino)pyrrolidine-1-carboxylate

[0336] The title compound (128 mg) was prepared in the same fashion as Step 1 of Reference Example 17, except that 7-bromobenzo[b]thiophene (685.0 mg, 3.22 mmol) was used instead of 3- bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.21-7.45 (m, 4H), 6.57-6.62 (m, 1H), 4.21-4.31 (m, 1H), 3.73-3.82 (m, 2H), 3.44-3.60 (m, 2H), 3.26-3.40 (m, 1H), 2.23-2.25 (m, 1H), 1.97-2.21 (m, 1H), 1.40-1.56 (m, 9H)

[0337] Step 2: (R)-N-(benzo[b]thiophen-7-yl)pyrrolidin-3-amine hydrochloride

[0338] The title compound (79.0 mg) was prepared in the same fashion as Step 2 of Reference Example 5 and used in the next step without any further purification.

[0339] Reference Example 21. (S)-N-(quinolin-4-yl)pyrrolidine-3-carboxamide hydrochloride

[0340] Step 1: tert-butyl (S)-3-(quinolin-4-y1cabamoyl)pyrrolidine-1-carboxylate

[0341] (S)-1-(Tert-butoxycarbonyl)pyrrolidine-3-carboxylic acid (300 mg, 1.39 mmol) was dissolved in dichloromethane. To the resulting solution, 4-aminoquinoline (200.9 mg, 1.39 mmol), HATU (O-(7-azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluranium hexafluorophosphate) (794.9 mg, 2.09 mmol), and diisopropylethylamine (0.49 mL, 2.79 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol= 20/1, v/v) to give the title compound (250 mg). ¹ H-NMR (CDCI ₃ , 400MHz) δ 9.05 (s, 1H), 8.80 (s, 1H), 8.63 (s, 1H), 8.38 (s, 1H), 7.95 (d, 2H), 7.71 (s, 1H), 3.71-3.46 (m, 5H), 2.83 (s, 10H), 2.32-2.18 (m, 2H), 1.50-1.43 (d, 9H)

[0342] Step 2: (S)-N-(quinolin-4-yl)pyrrolidine-3-carboxamide hydrochloride

[0343] Tert-butyl (S)-3-(quinolin-4-y1cabamoyl)pyrrolidine-1-carboxylate (450 mg, 1.32 mmol) prepared in Step 1 was dissolved in dichloromethane (7.3 ml) and then 4.0 M of hydrochloric acid dissolved in 1,4-dioxane was added thereto in excess drops. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, the solid produced therefrom was filtered and dried to give the title compound (220 mg). The title compound was used in the next step without any further purification. [0344] Reference Example 22. (S)-N-(quinolin-3-yl)pyrrolidine-3-carboxamide hydrochloride

[0345] Step 1: tert-butyl (S)-3-(quinolin-3-y1cabamoyl)pyrrolidine-1-carboxylate

[0346] The title compound (100 mg) was prepared in the same fashion as Step 1 of Reference Example 21, except that 3-aminoquinoline (200.9 mg, 1.39 mmol) was used instead of 4- aminoquinoline. MS (ESI) m/z= 342.4 (M + H)+

[0347] Step 2: (S)-N-(quinolin-3-yl)pyrrolidine-3-carboxamide hydrochloride

[0348] The title compound (190.7 mg) was prepared in the same fashion as Step 2 of Reference Example 21 and used in the next step without any further purification.

[0349] Reference Example 23. (R)-N-(quinolin-4-yl)pyrrolidine-3-carboxamide hydrochloride

[0350] Step 1: tert-butyl (R)-3-(quinolin-4-y1cabamoyl)pyrrolidine-1-carboxylate

[0351] The title compound (340 mg) was prepared in the same fashion as Step 1 of Reference Example 21 except that (R)-1-(tert-butoxycarbonyl)pyrrolidine-3-carboxylic acid (300 mg, 1.39 mmol) was used instead of (S)-1-(tert-butoxycarbonyl)pyrrolidine-3-carboxylic acid. ¹ H-NMR (CDCI ₃ , 400MHz) δ 9.05 (s, 1H), 8.80 (s, 1H), 8.63 (s, 1H), 8.38 (s, 1H), 7.95 (d, 2H), 7.71 (s, 1H), 3.71-3.46 (m, 5H), 2.83 (s, 10H), 2.32-2.18 (m, 2H), 1.50-1.43 (d, 9H)

[0352] Step 2: (R)-N-(quinolin-4-yl)pyrrolidine-3-carboxamide hydrochloride

[0353] The title compound (260 mg) was prepared in the same fashion as Step 2 of Reference Example 21 and used in the next step without any further purification.

[0354] Reference Example 24. (R)-N-(quinolin-3-yl)pyrrolidine-3-carboxamide hydrochloride

[0355] Step 1: tert-butyl (R)-3-(quinolin-3-y1cabamoyl)pyrrolidine-1-carboxylate

[0356] The title compound (450 mg) was prepared in the same fashion as Step 1 of Reference Example 21, except that 3-aminoquinoline (200.9 mg, 1.39 mmol) was used instead of 4- aminoquinoline. MS (ESI) m/z= 342.4 (M + H)+

[0357] Step 2: (R)-N-(quinolin-3-yl)pyrrolidine-3-carboxamide hydrochloride

[0358] The title compound (310.7 mg) was prepared in the same fashion as Step 2 of Reference

Example 21 and used in the next step without any further purification.

[0359] Reference Example 25. (S)-1-(2-((S)-3-aminopyrrolidin-1-yl)acetyl)pyrrolidine-2- carbonitrile trifluoroacetate

[0360] Step 1: tert-butyl ((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolidin -3- yl)carbamate [0361] (S)-1-(2-Chloroacetyl)pyrrolidine-2-carbonitrile(3 g, 17.4 mmol) prepared in Reference Example 1 was dissolved in anhydrous dichloromethane (34.8 ml). To the resulting solution, (3S)-(-)- 3-(tert-butoxycarbonylamino)pyrrolidine (3.56 g, 19.1 mmol), and potassium carbonate (4.8 g, 34.8 mmol) was added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 4/1, v/v) to give the title compound (2.33 g). ¹ H-

NMR (CDCI ₃ , 400MHz) δ 4.77-4.82 (m, 1H), 3.60-3.74 (m, 1H), 3.30-3.55 (m, 6H), 3.05-3.28 (m, 1H), 2.10-2.44 (m, 4H), 1.68-1.86 (m, 4H), 1.41-1.54 (m, 9H)

[0362] Step 2: (S)-1-(2-((S)-3-aminopyrrolidin-1-yl)acetyl)pyrrolidine-2-ca rbonitrile trifluoroacetate

[0363] Tert-butyl ((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolidin -3-yl)carbamate

(2.6 g, 8.06 mmol) prepared in Step 1 was dissolved in dichloromethane (44.8 ml) and trifluoroacetic acid was added thereto in excess drops. The reaction mixture was stirred overnight at room temperature and concentrated to give the title compound (1.3 g). ¹ H-NMR (CD ₃ OD, 400MHz) δ 4.79-4.91 (m, 1H), 4.41 (q, 2H), 4.09-4.21 (m, 1H), 3.96-4.05 (m, 1H), 3.54-3.81 (m, 5H), 2.55-2.67 (m, 1H), 2.10-2.31 (m, 5H)

[0364] Reference Example 26. (S)-1-(2-((R)-3-hydroxypyrrolidin-1-yl)acetyl)pyrrolidine-2- carbonitrile

[0365] (S)-1-(2-Chloroacetyl)pyrrolidine-2-carbonitrile (2 g, 11.6 mmol) prepared in Reference

Example 1 was dissolved in anhydrous acetonitrile (23.2 ml). To the resulting solution, (R)-3- pyrrolidinol (1.12 mL, 13.9 mmol) and potassium carbonate (4 g, 28.9 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, the reaction mixture was filtered. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 1/1, v/v) to give the title compound (2.42 g). ¹ H-

NMR (CDC1 ₃ , 400MHz) δ 4.74(d, 1H), 4.35(s, 1H), 3.65-3.63(m, 1H), 3.51-3.33(m, 3H), 3.11- 2.96(m, 2H), 2.88-2.80(m, 2H), 2.55-2.47(m, 1H), 2.35-2.16(m, 5H), 1.90-1.75(m, 1H)

[0366] Reference Example 27. (S)-4,4-difluoro-1-(2-((R)-3-hydroxypyrrolidin-1-yl)-2- oxoethyl)pyrrolidine-2-carbonitrile

[0367] The title compound (2.52 g) was prepared in the same fashion as Reference Example 26, except that (2S)-1-(2-chloroacetyl)-4,4-difluoro-pyrrolidine-2-carbonitr ile (1.4 g, 11.6 mmol) prepared in Reference Example 3 was used instead of (S)-1-(2-chloroacetyl)pyrrolidine-2-carbonitrile. MS (ESI) m/z= 260.2 (M + H)+ [0368] Reference Example 28. N-(piperidin-4-yl)quinolin-4-amine hydrochloride

[0369] Step 1: tert-butyl 4-(quinolin-4-ylamino)piperidine-1-carboxylate

[0370] Tert-butyl 4-aminopiperidine-1-carboxylate (500 mg, 2.68 mmol) was dissolved in anhydrous toluene. To the resulting solution, 4-bromoquinoline (670.2 mg, 3.22 mmol), cesium carbonate (1.31 g, 4.02 mmol), tris(dibenzylideneacetone)dipalladium(0) (245.8 mg, 0.27 mmol), and (+/-)-2,2'-bis(diphenylphospino)-1,1'-binaphthalene (167.1 mg, 0.27 mmol) were added. The reaction mixture was refluxed and stirred at 100°C overnight. After the completion of the reaction, the reaction mixture was cooled to room temperature and then added with water to extract an aqueous layer with ethyl acetate. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 1/1, v/v) to give the title compound (720 mg). ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.56 (d, 1H), 7.99 (d, 1H), 7.72 (d, 1H), 7.63 (t, 1H), 7.43 (t, 1H), 6.47 (d, 1H), 4.93 (d, 1H), 4.12 (d, 2H), 3.68 (s, 1H), 3.00 (t, 1H), 2.17-2.14 (m, 2H), 1.53-1.44 (m, 12H)

[0371] Step 2: N-(piperidin-4-yl)quinolin-4-amine hydrochloride

[0372] Tert-butyl 4-(quinolin-4-ylamino)piperidine-1-carboxylate (720 mg, 2.20 mmol) prepared in Step 1 was dissolved in dichloromethane (12.2 ml). To the resulting solution, 4.0 M of hydrochloric acid dissolved in 1,4-dioxane was added in excess drops. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, the solid produced therefrom was filtered and dried to give the title compound (320 mg). The title compound was used in the next step without any further purification.

[0373] Reference Example 29. N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[0374] Step 1: tert-butyl 4-(quinolin-5-ylamino)piperidine-1-carboxylate

[0375] The title compound (500 mg) was prepared in the same fashion as Step 1 of Reference Example 28, except that 5-bromoquinoline (500 mg, 2.50 mmol) was used instead of 4- bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.87 (s, 1H), 8.15 (d, 1H), 7.58-7.49 (m, 2H), 7.34- 7.32 (m, 1H), 6.69 (d, 1H), 4.17 (d, 3H), 3.64 (s, 1H), 3.02 (t, 2H), 2.18-2.15 (m, 2H), 1.49-1.45 (m, 11H)

[0376] Step 2: N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[0377] The title compound (390 mg) was prepared in the same fashion as Step 2 of Reference Example 28 and used in the next step without any further purification.

[0378] Reference Example 30. N-(piperidin-4-yl)furo[3,2-c]pyridin-7-amine hydrochloride

[0379] The title compound (324 mg) was prepared in the same fashion as Reference Example 28, except that 7-bromofuro[3,2-c]pyridine (495.1 mg, 2.50 mmol) was used instead of 4-bromoquinoline in Step 1. ¹ H-NMR (CD ₃ OD, 400MHz) δ 8.6 (s, 1H), 8.26 (s, 1H), 8.17 (s, 1H), 7.29 (s, 1H), 4.00 (s, 1H), 3.52 (d, 2H), 3.24 (t, 2H), 2.32 (d, 2H), 1.91 (q, 2H)

[0380] Reference Example 31. 3-(piperidin-4-yloxy)quinobne hydrochloride

[0381] Step 1: tert-butyl 4-(quinolin-3-yloxy)piperidine-1-carboxylate

[0382] Tert-butyl 4-hydroxypiperidine-1-carboxylate (200 mg, 1.0 mmol) was dissolved in tetrahydrofuran (4.97 ml). To the resulting solution, 3-quinolinol (144.25 mg, 1.0 mmol) and triphenylphosphine (391 mg, 1.5 mmol) were added. The mixture solution was cooled to 0°C and then, DIAD (diisopropyl azodicarboxylate) (0.29 mL, 1.5 mmol) was slowly added thereto dropwise for 15 minutes. The reaction mixture was stirred for 12 hours at room temperature. After the completion of the reaction, a saturated ammonium chloride solution and a saturated sodium hydrogen carbonate solution were added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with ethyl acetate. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol= 10/1, v/v) to give the title compound (280 mg). MS (ESI) m/z= 330.2 (M + H)+

[0383] Step 2: 3-(piperidin-4-yloxy)quinoline hydrochloride

[0384] Tert-butyl 4-(quinolin-3-yloxy)piperidine-1-carboxylate (280 mg, 0.85 mmol) was dissolved in dichloromethane (4.7 ml). To the resulting solution, 4.0 M of hydrochloric acid dissolved in 1,4-dioxane was added in excess drops. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, the solid produced therefrom was filtered and dried to give the title compound (47 mg). The title compound was used in the next step without any further purification. MS (ESI) m/z= 230.1 (M + H)+

[0385] Reference Example 32. 4-(piperidin-4-yloxy)quinoline hydrochloride

[0386] The title compound (100 mg) was prepared in the same fashion as Reference Example 31, except that 4-quinolinol (144.25 mg, 1.0 mmol) was used instead of 3-quinolinol in Step 1. MS (ESI) m/z= 230.1 (M + H)+

[0387] Reference Example 33. 5-(piperidin-4-yloxy)quinoline hydrochloride

[0388] The title compound (60 mg) was prepared in the same fashion as Reference Example 31, except that 5-quinolinol (144.25 mg, 1.0 mmol) was used instead of 3-quinolinol in Step 1. The title compound was used in the next step without any further purification. MS (ESI) m/z= 230.1 (M + H)+ [0389] Reference Example 34. 6-(piperidin-4-yloxy)quinoline hydrochloride

[0390] The title compound (40 mg) was prepared in the same fashion as Reference Example 31, except that 6-quinolinol (144.25 mg, 1.0 mmol) was used instead of 3-quinolinol in Step 1. The title compound was used in the next step without any further purification. MS (ESI) m/z= 230.1 (M + H)+

[0391] Reference Example 35. 3-methyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[0392] The title compound (81.8 mg) was prepared in the same fashion as Reference Example 28, except that 5-bromo-3-methylquinoline (554.4 mg, 2.50 mmol) was used instead of 4-bromoquinoline in Step 1. The title compound was used in the next step without any further purification. MS (ESI) m/z= 242.3 (M + H)+

[0393] Reference Example 36. 6-methyl -N-(piperidin-4-yl)quinolin-5 -amine hydrochloride

[0394] The title compound (40.2 mg) was prepared in the same fashion as Reference Example 28, except that 5-bromo-6-methylquinoline (554.4 mg, 2.50 mmol) was used instead of 4-bromoquinoline in Step 1. The title compound was used in the next step without any further purification. MS (ESI) m/z= 242.3 (M + H)+

[0395] Reference Example 37. 7-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[0396] The title compound (251 mg) was prepared in the same fashion as Reference Example 28, except that 5-bromo-7-fluoroquinoline (564 mg, 2.50 mmol) was used instead of 4-bromoquinoline in Step 1. The title compound was used in the next step without any further purification. MS (ESI) m/z= 246.3 (M + H)+

[0397] Reference Example 38. 7-methoxy-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[0398] The title compound (106 mg) was prepared in the same fashion as Reference Example 28, except that 5-bromo-7-methoxyquinoline (594 mg, 2.50 mmol) was used instead of 4-bromoquinoline in Step 1. The title compound was used in the next step without any further purification. MS (ESI) m/z= 258.3 (M + H)+

[0399] Reference Example 39. 8-methoxy-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[0400] The title compound (27 mg) was prepared in the same fashion as Reference Example 28, except that 5-bromo-8-methoxyquinoline (594 mg, 2.50 mmol) was used instead of 4-bromoquinoline in Step 1. The title compound was used in the next step without any further purification. MS (ESI) m/z= 258.3 (M + H)+

[0401] Reference Example 40. 6-fluoro-4-(piperidin-4-yloxy)quinoline hydrochloride [0402] The title compound (214 mg) was prepared in the same fashion as Reference Example 31, except that 6-fluoroquinolin-4-ol (202.6 mg, 1.2 mmol) was used instead of 3-quinolinol in Step 1. The title compound was used in the next step without any further purification. MS (ESI) m/z= 246.3 (M + H)+

[0403] Reference Example 41. 6-chloro-4-(piperidin-4-yloxy)quinoline hydrochloride

[0404] The title compound (197 mg) was prepared in the same fashion as Reference Example 31, except that 6-chloroquinolin-4-ol (223.1 mg, 1.2 mmol) was used instead of 3-quinolinol in Step 1. The title compound was used in the next step without any further purification. MS (ESI) m/z= 262.8 (M + H)+

[0405] Reference Example 42. 6-methoxy-4-(piperidin-4-yloxy)quinoline hydrochloride

[0406] The title compound (108.6 mg) was prepared in the same fashion as Reference Example 31, except that 6-methoxyquinolin-4-ol (217.6 mg, 1.2 mmol) was used instead of 3-quinolinol in Step 1. The title compound was used in the next step without any further purification. MS (ESI) m/z= 258.3 (M + H)+

[0407] Reference Example 43. 7-methoxy-4-(piperidin-4-yloxy)quinoline hydrochloride

[0408] The title compound (39.2 mg) was prepared in the same fashion as Reference Example 31, except that 7-methoxyquinolin-4-ol (217.6 mg, 1.2 mmol) was used instead of 3-quinolinol in Step 1. The title compound was used in the next step without any further purification. MS (ESI) m/z= 258.3 (M + H)+

[0409] Reference Example 44. 6,7-dimethoxy-4-(piperidin-4-yloxy)quinoline hydrochloride

[0410] The title compound (24.7 mg) was prepared in the same fashion as Reference Example 31, except that 6,7-dimethoxyquinolin-4-ol (254.9 mg, 1.2 mmol) was used instead of 3-quinolinol in Step 1. The title compound was used in the next step without any further purification. MS (ESI) m/z= 288.3 (M + H)+

[0411] Reference Example 45. (S)-6-fluoro-N-(pyrrolidin-3-yl)benzofuran-3-carboxamide hydrochloride

[0412] Step 1: tert-butyl (S)-3-(6-fluorobenzofuran-3-carboxamido)pyrrolidin-1-carboxy late

[0413] Tert-butyl (S)-3-aminopyrrolidin-1-carboxylate (500 mg, 2.68 mmol) was dissolved in dimethylformamide (5.4 ml). To the resulting solution, 6-fluorobenzofuran-3 -carboxylic acid (725.4 mg, 4.02 mmol), HATU(O-(7-azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate) (1541.4 mg, 4.05 mmol) and diisopropylethylamine (4.67mL, 26.8 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with ethyl acetate. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (hexane/ethyl acetate = 1/4, v/v) to give the title compound (766 mg). 1H NMR (CDC13, 400MHz) δ 8.17 (s, 1H), 7.95 (s, 1H), 7.22 (d, 1H), 7.10 (t, 1H), 6.73-6.52 (m, 1H), 4.67 (s, 1H), 3.70 (d, 1H), 3.47 (d, 2H), 3.32 (s, 1H), 2.23 (s, 1H), 2.00 (d, 1H), 1.46 (s, 9H)

[0414] Step 2: (S)-6-fluoro-N-(pyrrolidin-3-yl)benzofuran-3-carboxamide hydrochloride

[0415] Tert-butyl (S)-3-(6-fluorobenzofuran-3-carboxamido)pyrrolidin-1-carboxy late (280 mg,

0.85 mmol) was dissolved in dichloromethane (4.7 ml) and then 4.0 M of hydrochloric acid dissolved in 1,4-dioxane was added thereto in excess drops. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, the solid produced therefrom was filtered and dried to give the title compound (47 mg). The title compound was used in the next step without any further purification. MS (ESI) m/z= 249.2 (M + H)+

[0416] Reference Example 46. (S)-6-methoxy-N-(pyrrolidin-3-yl)benzofuran-3-carboxamide hydrochloride

[0417] Step 1: tert-butyl (S)-3-(6-methoxybenzofuran-3-carboxamido)pyrrolidine-1-carbo xylate

[0418] The title compound (399.3 mg, 41.3 %) was prepared in the same fashion as Step 1 of

Reference Example 45, except that 6-methoxybenzofuran-3-carboxylic acid (772.5 mg, 4.02 mmol) was used instead of 6-fluorobenzofuran-3 -carboxyl acid. ¹ H NMR (CDCI ₃ , 400MHz) δ 8.06 (s, 1H), 7.78 (d, 1H), 7.03 (s, 1H), 6.97 (d, 1H), 4.68 (s, 1H), 3.86 (s, 3H), 3.72 (s, 1H), 3.51-3.32 (m, 3H), 2.26-2.25 (m, 1H), 1.98-1.83 (m, 2H), 1.47 (s, 9H).

[0419] Step 2: (S)-6-methoxy-N-(pyrrolidin-3-yl)benzofuran-3-carboxamide hydrochloride

[0420] The title compound (295.9 mg, 90.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification. ¹ H NMR (MeOD ₄ , 400MHz) δ 8.35 (s, 1H), 7.90 (d, 1H), 7.14 (s, 1H), 697 (d, 1H), 4.63-4.62 (m, 1H), 3.85 (s, 3H), 3.61-3.58 (m, 2H), 3.43-3.41 (m, 2H), 2.45-2.40 (m, 1H), 2.23-2.22 (m, 1H).

[0421] Reference Example 47. (R)-N-methyl-N-(pyrrolidin-3-yl)quinolin-2 -amine hydrochloride

[0422] Step 1: tert-butyl (R)-3-(methyl(quinolin-2-yl)amino)pyrrolidine-1-carboxylate

[0423] (R) -tert-butyl 3-(methylamino)pyrrolidine-1-carboxylate (250.0 mg, 1.25 mmol) was dissolved in anhydrous toluene. To the resulting solution, 2-bromoquinoline (259.7 mg, 1.25 mmol), cesium carbonate (488.1 mg, 1.50 mmol), tris(dibenzylideneacetone)dipalladium(0) (114.3 mg, 0.13 mmol), and (+/-)-2,2'-bis(diphenylphospino)-1,1'-binaphthalene (93.3 mg, 0.15 mmol) were added. The reaction mixture was refluxed and stirred at 100 °C overnight. After the completion of the reaction, the reaction mixture was cooled to room temperature and then added with water to extract an aqueous layer with ethyl acetate. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 1/1, v/v) to give a title compound (56.4 mg, 13.8 %). MS (ESI) m/z= 328.2 (M + H)+

[0424] Step 2: (R)-N-methyl-N-(pyrrolidin-3-yl)quinolin-2 -amine hydrochloride

[0425] The title compound (25.5 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0426] Reference Example 48. (R)-N-methyl-N-(pyrrolidin-3-yl)quinolin-3 -amine hydrochloride

[0427] Step 1: tert-butyl (R)-3-(methyl(quinolin-3-yl)amino)pyrrolidine-1-carboxylate

[0428] The title compound (220.4 mg, 53.9 %) was prepared in the same fashion as Step 1 of

Reference Example 47, except that 3-bromoquinoline (259.7 mg, 1.25 mmol) was used instead of 2- bromoquinoline. MS (ESI) m/z= 328.2 (M + H)+

[0429] Step 2: (R)-N-methyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride

[0430] The title compound (168.8 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0431] Reference Example 49. (R)-N-methyl-N-(pyrrolidin-3-yl)quinolin-5 -amine hydrochloride

[0432] Step 1: tert-butyl (R)-3-(methyl(quinolin-5-yl)amino)pyrrolidine-1-carboxylate

[0433] The title compound (109.4 mg, 26.8 %) was prepared in the same fashion as Step 1 of

Reference Example 47, except that 5-bromoquinoline (259.7 mg, 1.25 mmol) was used instead of 2- bromoquinoline. MS (ESI) m/z= 328.1 (M + H)+

[0434] Step 2: (R)-N-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0435] The title compound (88.1 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0436] Reference Example 50. (R)-N-methyl-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0437] Step 1: tert-butyl (R)-3-(methyl(quinolin-6-yl)amino)pyrrolidine-1-carboxylate

[0438] The title compound (73.9 mg, 18.1 %) was prepared in the same fashion as Step 1 of

Reference Example 47, except that 6-bromoquinoline (259.7 mg, 1.25 mmol) was used instead of 2- bromoquinoline. MS (ESI) m/z= 328.3 (M + H)+ [0439] Step 2: (R)-N-methyl-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0440] The title compound (59.5 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0441] Reference Example 51. (R)-N-methyl-N-(pyrrolidin-3-yl)quinolin-7-amine hydrochloride

[0442] Step 1: tert-butyl (R)-3-(methyl(quinolin-7-yl)amino)pyrrolidine-1-carboxylate

[0443] The title compound (131.9 mg, 32.3 %) was prepared in the same fashion as Step 1 of

Reference Example 47, except that 7-bromoquinoline (259.7 mg, 1.25 mmol) was used instead of 2- bromoquinoline. MS (ESI) m/z= 358.2 (M + H)+

[0444] Step 2: (R)-N-methyl-N-(pyrrolidin-3-yl)quinolin-7-amine hydrochloride

[0445] The title compound (106.3 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0446] Reference Example 52. (S)-N-methyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride

[0447] Step 1: tert-butyl (S)-3 -(methyl (quinolin-3-yl)amino)pyrrolidine-1-carboxylate

[0448] (S)-tert-butyl 3-(methylamino)pyrrolidine-1-carboxylate (250.0 mg, 1.25 mmol) was dissolved in anhydrous toluene. To the resulting solution, 3-bromoquinoline (259.7 mg, 1.25 mmol), cesium carbonate (488.1 mg, 1.50 mmol), tris(dibenzylideneacetone)dipalladium(0) (114.3 mg, 0.13 mmol), and (+/-)-2,2'-bis(diphenylphospino)-1,1'-binaphthalene (93.3 mg, 0.15 mmol) were added. The reaction mixture was refluxed and stirred at 100 °C overnight. After the completion of the reaction, the reaction mixture was cooled to room temperature and then added with water to extract an aqueous layer with ethyl acetate. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 1/1, v/v) to give atitle compound (252.5 mg, 61.8 %). MS (ESI) m/z= 328.3 (M + H)+

[0449] Step 2: (S)-N-methyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride

[0450] The title compound (203.4 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0451] Reference Example 53. (S)-N-methyl-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0452] Step 1: tert-butyl (S)-3 -(methyl (quinolin-4-yl)amino)pyrrolidine-1-carboxylate

[0453] The title compound (74.2 mg, 18.2 %) was prepared in the same fashion as Step 1 of

Reference Example 52, except that 4-bromoquinoline (259.7 mg, 1.25 mmol) was used instead of 3- bromoquinoline. MS (ESI) m/z= 328.3 (M + H)+ [0454] Step 2: (S)-N-methyl-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0455] The title compound (59.8 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0456] Reference Example 54. (S)-N-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0457] Step 1: tert-butyl (S)-3 -(methyl (quinolin-5-yl)amino)pyrrolidine-1-carboxylate

[0458] The title compound (124.2 mg, 30.4 %) was prepared in the same fashion as Step 1 of

Reference Example 52, except that 5-bromoquinoline (259.7 mg, 1.25 mmol) was used instead of 3- bromoquinoline. MS (ESI) m/z= 328.2 (M + H)+

[0459] Step 2: (S)-N-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0460] The title compound (100.1 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0461] Reference Example 55. (S)-N-methyl-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0462] Step 1: tert-butyl (S)-3 -(methyl (quinolin-6-yl)amino)pyrrolidine-1-carboxylate

[0463] The title compound (120.5 mg, 29.5 %) was prepared in the same fashion as Step 1 of

Reference Example 52, except that 6-bromoquinoline (259.7 mg, 1.25 mmol) was used instead of 3- bromoquinoline. MS (ESI) m/z= 328.3 (M + H)+

[0464] Step 2: (S)-N-methyl-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0465] The title compound (97.1 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0466] Reference Example 56. (S)-N-methyl-N-(pyrrolidin-3-yl)quinolin-7-amine hydrochloride

[0467] Step 1: tert-butyl (S)-3 -(methyl (quinolin-7-yl)amino)pyrrolidine-1-carboxylate

[0468] The title compound (260.0 mg, 63.6 %) was prepared in the same fashion as Step 1 of

Reference Example 52, except that 7-bromoquinoline (259.7 mg, 1.25 mmol) was used instead of 3- bromoquinoline. MS (ESI) m/z= 328.2 (M + H)+

[0469] Step 2: (S)-N-methyl-N-(pyrrolidin-3-yl)quinolin-7-amine hydrochloride

[0470] The title compound (209.5 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0471] Reference Example 57. (S)-N-methyl-N-(pyrrolidin-3-yl)isoquinolin-4-amine hydrochloride

[0472] Step 1: tert-butyl (S)-3-(isoquinolin-4-yl(methyl)amino)pyrrolidine-1-carboxyla te [0473] The title compound (69.0 mg, 16.9 %) was prepared in the same fashion as Step 1 of

Reference Example 52, except that 4-bromoisoquinoline (259.7 mg, 1.25 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 328.3 (M + H)+

[0474] Step 2: (S)-N-methyl-N-(pyrrolidin-3-yl)isoquinolin-4-amine hydrochloride

[0475] The title compound (55.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0476] Reference Example 58. (S)-N-methyl-N-(pyrrolidin-3-yl)isoquinolin-5-amine hydrochloride

[0477] Step 1: tert-butyl (S)-3-(isoquinolin-5-yl(methyl)amino)pyrrolidine-1-carboxyla te

[0478] The title compound (87.8 mg, 21.5 %) was prepared in the same fashion as Step 1 of

Reference Example 52, except that 5-bromoisoquinoline (259.7 mg, 1.25 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 328.3 (M + H)+

[0479] Step 2: (S)-N-methyl-N-(pyrrolidin-3-yl)isoquinolin-5-amine hydrochloride

[0480] The title compound (70.7 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0481] Reference Example 59. (S)-N,4-dimethyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride

[0482] Step 1: tert-butyl (S)-3 -(methyl (4-methylquinolin-3-yl)amino)pyrrolidine-1-carboxylate

[0483] The title compound (60.4 mg, 14.2 %) was prepared in the same fashion as Step 1 of

Reference Example 52, except that 3-bromo-4-methylquinoline (277.2 mg, 1.25 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 342.2 (M + H)+

[0484] Step 2: (S)-N,4-dimethyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride

[0485] The title compound (49.1 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0486] Reference Example 60. (S)-N,6-dimethyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride

[0487] Step 1: tert-butyl (S)-3-(methyl(6-methylquinolin-3-yl)amino)pyrrolidine-1-carb oxylate

[0488] The title compound (244.0 mg, 57.3 %) was prepared in the same fashion as Step 1 of

Reference Example 52, except that 3-bromo-6-methylquinoline (277.2 mg, 1.25 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 342.2 (M + H)+ [0489] Step 2: (S)-N,6-dimethyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride

[0490] The title compound (198.5 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0491] Reference Example 61. (S)-6-fluoro-N-methyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride

[0492] Step 1: tert-butyl (S)-3-((6-fluoroquinolin-3-yl)(methyl)amino)pyrrolidine-1-ca rboxylate

[0493] The title compound (314.7 mg, 73.0 %) was prepared in the same fashion as Step 1 of

Reference Example 52, except that 3-bromo-6-fluoroquinoline (282.2 mg, 1.25 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 346.3 (M + H)+

[0494] Step 2: (S)-6-fluoro-N-methyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride

[0495] The title compound (256.7 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0496] Reference Example 62. (S)-6-methoxy-N-methyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride

[0497] Step 1: tert-butyl (S)-3-((6-methoxyquinolin-3-yl)(methyl)amino)pyrrolidine-1- carboxylate

[0498] The title compound (264.3 mg, 59.2 %) was prepared in the same fashion as Step 1 of

Reference Example 52, except that 3 -bromo-6-methoxy quinoline (297.2 mg, 1.25 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 358.2 (M + H)+

[0499] Step 2: (S)-6-methoxy-N-methyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride

[0500] The title compound (217.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0501] Reference Example 63. (S)-7-methoxy-N-methyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride

[0502] Step 1: tert-butyl (S)-3-((7-methoxyquinolin-3-yl)(methyl)amino)pyrrolidine-1- carboxylate

[0503] The title compound (177.0 mg, 39.7 %) was prepared in the same fashion as Step 1 of

Reference Example 52, except that 3 -bromo-7-methoxy quinoline (297.2 mg, 1.25 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 358.2 (M + H)+

[0504] Step 2: (S)-7-methoxy-N-methyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride [0505] The title compound (145.5 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0506] Reference Example 64. (S)-8-methoxy-N-methyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride

[0507] Step 1: tert-butyl (S)-3-((8-methoxyquinolin-3-yl)(methyl)amino)pyrrolidine-1- carboxylate

[0508] The title compound (359.9 mg, 80.7 %) was prepared in the same fashion as Step 1 of

Reference Example 52, except that 3-bromo-8-methoxyquinoline (297.2 mg, 1.25 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 358.2 (M + H)+

[0509] Step 2: (S)-8-methoxy-N-methyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride

[0510] The title compound (295.8 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0511] Reference Example 65. (S)-N,3-dimethyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0512] Step 1: tert-butyl (S)-3-(methyl(3-methylquinolin-5-yl)amino)pyrrolidine-1-carb oxylate

[0513] The title compound (115.1 mg, 27.0 %) was prepared in the same fashion as Step 1 of

Reference Example 52, except that 5-bromo-3-methylquinoline (277.2 mg, 1.25 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 342.2 (M + H)+

[0514] Step 2: (S)-N,3-dimethyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0515] The title compound (93.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0516] Reference Example 66. (S)-3-fluoro-N-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0517] Step 1: tert-butyl (S)-3-((3-fluoroquinolin-5-yl)(methyl)amino)pyrrolidine-1-ca rboxylate

[0518] The title compound (286.6 mg, 66.5 %) was prepared in the same fashion as Step 1 of

Reference Example 52, except that 5-bromo-3-fluoro-quinoline (282.2 mg, 1.25 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 346.2 (M + H)+

[0519] Step 2: (S)-3-fluoro-N-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0520] The title compound (233.8 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification. [0521] Reference Example 67. (S)-7-fluoro-N-metliyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0522] Step 1: tert-butyl (S)-3-((7-fluoroquinolin-5-yl)(methyl)amino)pyrrolidine-1-ca rboxylate

[0523] The title compound (223.7 mg, 51.9 %) was prepared in the same fashion as Step 1 of

Reference Example 52, except that 5-bromo-7-fluoro-quinoline (282.2 mg, 1.25 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 346.2 (M + H)+

[0524] Step 2: (S)-7-fluoro-N-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0525] The title compound (182.5 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0526] Reference Example 68. (S)-8-fluoro-N-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0527] Step 1: tert-butyl (S)-3-((8-fluoroquinolin-5-yl)(methyl)amino)pyrrolidine-1-ca rboxylate

[0528] The title compound (209.9 mg, 48.7 %) was prepared in the same fashion as Step 1 of

Reference Example 52, except that 5-bromo-8-fluoro-quinoline (282.2 mg, 1.25 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 346.2 (M + H)+

[0529] Step 2: (S)-8-fluoro-N-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0530] The title compound (171.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0531] Reference Example 69. (S)-N-methyl-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin -5- amine hydrochloride

[0532] Step 1: tert-butyl (S)-3-(methyl(8-(trifluoromethyl)quinolin-5-yl)amino)pyrroli dine-1- carboxylate

[0533] The title compound (416.4 mg, 84.4 %) was prepared in the same fashion as Step 1 of

Reference Example 52, except that 5-bromo-8-(trifluoromethyl)quinoline (344.6 mg, 1.25 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 396.2 (M + H)+

[0534] Step 2: (S)-N-methyl-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin -5-amine hydrochloride

[0535] The title compound (349.4 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0536] Reference Example 70. (S)-N-methyl-N-(pyrrolidin-3-yl)-8-(trifluoromethoxy)quinoli n-5- amine hydrochloride [0537] Step 1: tert-butyl (S)-3-(methyl(8-(trifluoromethoxy)quinolin-5-yl)amino)pyrrol idine-1- carboxylate

[0538] The title compound (432.5 mg, 84.2 %) was prepared in the same fashion as Step 1 of

Reference Example 52, except that 5-bromo-8-trifluoromethoxyquinoline (364.6 mg, 1.25 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 412.2 (M + H)+

[0539] Step 2: (S)-N-methyl-N-(pyrrolidin-3-yl)-8-(trifluoromethoxy)quinoli n-5-amine hydrochloride

[0540] The title compound (365.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0541] Reference Example 71. (R)-N-(pyrrolidin-3-yl)quinolin-5 -amine hydrochloride

[0542] Step 1: tert-butyl (R)-3-(quinolin-5-ylamino)pyrrolidine-1-carboxylate

[0543] The title compound (589.6 mg, 70.0 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 5-bromoquinoline (558.5 mg, 2.68 mmol) was used instead of 3- bromoquinoline. MS (ESI) m/z= 314.2 (M + H)+

[0544] Step 2: (R)-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0545] The title compound (466.5 mg, 86.6 %) was prepared in the same fashion as Step 2 of

Reference Example 5. MS (ESI) m/z= 214.2 (M + H)+

[0546] Reference Example 72. (R)-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0547] Step 1: tert-butyl (R)-3-(quinolin-6-ylamino)pyrrolidine-1-carboxylate

[0548] The title compound (389.3 mg, 46.3 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 6-bromoquinoline (558.5 mg, 2.68 mmol) was used instead of 3- bromoquinoline. MS (ESI) m/z= 314.2 (M + H)+

[0549] Step 2: (R)-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0550] The title compound (298.1 mg, 94.1 %) was prepared in the same fashion as Step 2 of

Reference Example 5. MS (ESI) m/z= 214.2 (M + H)+

[0551] Reference Example 73. (R)-N-(pyrrolidin-3-yl)quinolin-7-amine hydrochloride

[0552] Step 1: tert-butyl (R)-3-(quinolin-7-ylamino)pyrrolidine-1-carboxylate

[0553] The title compound (356.5 mg, 42.4 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 7-bromoquinoline (558.5 mg, 2.68 mmol) was used instead of 3- bromoquinoline. MS (ESI) m/z= 314.2 (M + H)+ [0554] Step 2: (R)-N-(pyrrolidin-3-yl)quinolin-7-amine hydrochloride

[0555] The title compound (261.9 mg, 82.6 %) was prepared in the same fashion as Step 2 of

Reference Example 5. MS (ESI) m/z= 214.2 (M + H)+

[0556] Reference Example 74. (R)-N-(pyrrolidin-3-yl)quinolin-8-amine hydrochloride

[0557] Step 1: tert-butyl (R)-3-(quinolin-8-ylamino)pyrrolidine-1-carboxylate

[0558] The title compound (692.6 mg, 82.3 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 8-bromoquinoline (558.5 mg, 2.68 mmol) was used instead of 3- bromoquinoline. MS (ESI) m/z= 314.2 (M + H)+

[0559] Step 2: (R)-N-(pyrrolidin-3-yl)quinolin-8-amine hydrochloride

[0560] The title compound (589.4 mg, 93.1 %) was prepared in the same fashion as Step 2 of

Reference Example 5. MS (ESI) m/z= 214.2 (M + H)+

[0561] Reference Example 75. (R)-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0562] Step 1: tert-butyl (R)-3-(quinolin-4-ylamino)pyrrolidine-1-carboxylate

[0563] The title compound (490.9 mg, 58.4 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 4-bromoquinoline (558.5 mg, 2.68 mmol) was used instead of 3- bromoquinoline. MS (ESI) m/z= 314.2 (M + H)+

[0564] Step 2: (R)-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0565] The title compound (195.4 mg, 43.6 %) was prepared in the same fashion as Step 2 of

Reference Example 5. MS (ESI) m/z= 214.2 (M + H)+

[0566] Reference Example 76. (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3-amine hydrochloride

[0567] Step 1: tert-butyl (R)-3-((1,8-naphthyridin-3-yl)amino)pyrrolidine-1-carboxylat e

[0568] The title compound (278.2 mg, 65.9 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 3-bromo-1,8-naphthyridine (280.6 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 315.1 (M + H)+

[0569] Step 2: (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3-amine hydrochloride

[0570] The title compound (254.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0571] Reference Example 77. (R)-7-chloro-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3-amine hydrochloride

[0572] Step 1: tert-butyl (R)-3-((7-chloro-1,8-naphthyridin-3-yl)amino)pyrrolidine-1-c arboxylate [0573] The title compound (174.8 mg, 37.3 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 6-bromo-2-chloro-1,8-naphthyridine (326.8 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 349.1 (M + H)+

[0574] Step 2: (R)-7-chloro-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3-amine hydrochloride

[0575] The title compound (161.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0576] Reference Example 78. (R)-6-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0577] Step 1: tert-butyl (R)-3-((6-fluoroquinolin-5-yl)amino)pyrrolidine-1-carboxylat e

[0578] The title compound (187.1 mg, 42.1 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 5-bromo-6-fluoroquinoline (303.4 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 332.2 (M + H)+

[0579] Step 2: (R)-6-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0580] The title compound (171.9 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0581] Reference Example 79. (R)-7-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0582] Step 1: tert-butyl (R)-3-((7-fluoroquinolin-5-yl)amino)pyrrolidine-1-carboxylat e

[0583] The title compound (129.4 mg, 29.1 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 5-bromo-7-fluoroquinoline (303.4 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 332.2 (M + H)+

[0584] Step 2: (R)-7-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0585] The title compound (118.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0586] Reference Example 80. (R)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0587] Step 1: tert-butyl (R)-3-((8-fluoroquinolin-5-yl)amino)pyrrolidine-1-carboxylat e

[0588] The title compound (330.4 mg, 74.3 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 5-bromo-8-fluoroquinoline (303.4 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 332.2 (M + H)+

[0589] Step 2: (R)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0590] The title compound (303.3 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification. [0591] Reference Example 81. (R)-6-metlioxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0592] Step 1: tert-butyl (R)-3-((6-methoxyquinolin-5-yl)amino)pyrrolidine-1-carboxyla te

[0593] The title compound (427.4 mg, 92.7 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 5 -bromo-6-methoxy quinoline (319.6 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 344.2 (M + H)+

[0594] Step 2: (R)-6-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0595] The title compound (393.7 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0596] Reference Example 82. (R)-7-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0597] Step 1: tert-butyl (R)-3-((7-methoxyquinolin-5-yl)amino)pyrrolidine-1-carboxyla te

[0598] The title compound (460.9 mg, 100.0 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 5 -bromo-7-methoxy quinoline (319.6 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 344.2 (M + H)+

[0599] Step 2: (R)-7-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0600] The title compound (424.4 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0601] Reference Example 83. (R)-8-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0602] Step 1: tert-butyl (R)-3-((8-methoxyquinolin-5-yl)amino)pyrrolidine-1-carboxyla te

[0603] The title compound (180.0 mg, 39.1 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 5-bromo-8-methoxyquinoline (319.6 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 344.2 (M + H)+

[0604] Step 2: (R)-8-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0605] The title compound (165.7 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0606] Reference Example 84. (R)-3-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0607] Step 1: tert-butyl (R)-3-((3-methylquinolin-5-yl)amino)pyrrolidine-1-carboxylat e

[0608] The title compound (314.2 mg, 71.5 %) was prepared in the same fashion as Step 1 of Reference Example 17, except that 5-bromo-3-methylquinoline (298.1 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 328.2 (M + H)+

[0609] Step 2: (R)-3-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0610] The title compound (288.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0611] Reference Example 85. (R)-6-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0612] Step 1: tert-butyl (R)-3-((6-methylquinolin-5-yl)amino)pyrrolidine-1-carboxylat e

[0613] The title compound (419.0 mg, 95.3 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 5-bromo-6-methylquinoline (298.1 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 328.2 (M + H)+

[0614] Step 2: (R)-6-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0615] The title compound (384.3 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0616] Reference Example 86. (R)-8-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0617] Step 1: tert-butyl (R)-3-((8-methylquinolin-5-yl)amino)pyrrolidine-1-carboxylat e

[0618] The title compound (290.5 mg, 66.1 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 5-bromo-8-methylquinoline (298.1 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 328.2 (M + H)+

[0619] Step 2: (R)-8-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0620] The title compound (266.3 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0621] Reference Example 87. (R)-N-(pyrrolidin-3-yl)-7-(trifluoromethyl)quinolin-5-amine hydrochloride

[0622] Step 1: tert-butyl (R)-3-((7-(trifluoromethyl)quinolin-5-yl)amino)pyrrolidine-1 - carboxylate

[0623] The title compound (424.1 mg, 82.8 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 5-bromo-7-(trifluoromethyl)quinoline (370.5 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 382.2 (M + H)+

[0624] Step 2: (R)-N-(pyrrolidin-3-yl)-7-(trifluoromethyl)quinolin-5-amine hydrochloride

[0625] The title compound (393.9 mg, 100.0 %) was prepared in the same fashion as Step 2 of Reference Example 5 and used in the next step without any further purification.

[0626] Reference Example 88. (R)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-5-amine hydrochloride

[0627] Step 1: tert-butyl (R)-3-((8-(trifluoromethyl)quinolin-5-yl)amino)pyrrolidine-1 - carboxylate

[0628] The title compound (186.5 mg, 36.4 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 5-bromo-8-(trifluoromethyl)quinoline (370.5 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 382.2 (M + H)+

[0629] Step 2: (R)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-5-amine hydrochloride

[0630] The title compound (173.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0631] Reference Example 89. (R)-3-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0632] Step 1: tert-butyl (R)-3-((3-fluoroquinolin-5-yl)amino)pyrrolidine-1-carboxylat e

[0633] The title compound (251.6 mg, 56.6 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 5-bromo-3-fluoro-quinoline (303.4 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 332.2 (M + H)+

[0634] Step 2: (R)-3-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0635] The title compound (230.9 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0636] Reference Example 90. (R)-3-chloro-N-(pyrrolidin-3-yl)quinolin-5 -amine hydrochloride

[0637] Step 1: tert-butyl (R)-3-((3-chloroquinolin-5-yl)amino)pyrrolidine-1-carboxylat e

[0638] The title compound (320.1 mg, 68.6 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 5-bromo-3-chloro-quinoline (325.5 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 348.2 (M + H)+

[0639] Step 2: (R)-3-chloro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0640] The title compound (295.0 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0641] Reference Example 91. (R)-N-(pyrrolidin-3-yl)-8-(trifluoromethoxy)quinolin-5-amine hydrochloride

[0642] Step 1: tert-butyl (R)-3-((8-(trifluoromethoxy)quinolin-5-yl)amino)pyrrolidine- 1- carboxylate

[0643] The title compound (470.2 mg, 88.2 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 5-bromo-8-trifluoromethoxyquinoline (392.0 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 398.2 (M + H)+

[0644] Step 2: (R)-N-(pyrrolidin-3-yl)-8-(trifluoromethoxy)quinolin-5-amine hydrochloride

[0645] The title compound (438.0 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0646] Reference Example 92. (R)-6-methyl-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0647] Step 1: tert-butyl (R)-3-((6-methylquinolin-4-yl)amino)pyrrolidine-l -carboxylate

[0648] The title compound (47.1 mg, 10.7 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 4-bromo-6-methylquinoline (298.1 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 328.2 (M + H)+

[0649] Step 2: (R)-6-methyl-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0650] The title compound (43.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0651] Reference Example 93. (R)-6-methoxy-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0652] Step 1: tert-butyl (R)-3-((6-methoxyquinolin-4-yl)amino)pyrrolidine-1-carboxyla te

[0653] The title compound (326.8 mg, 70.9 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 4-bromo-6-methoxyquinoline (319.6 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 344.1 (M + H)+

[0654] Step 2: (R)-6-methoxy-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0655] The title compound (301.1 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0656] Reference Example 94. (R)-8-chloro-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0657] Step 1: tert-butyl (R)-3-((8-chloroquinolin-4-yl)amino)pyrrolidine-1-carboxylat e

[0658] The title compound (126.9 mg, 27.2 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 4-bromo-8-chloroquinoline (325.5 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 348.2 (M + H)+

[0659] Step 2: (R)-8-chloro-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride [0660] The title compound (117.0 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0661] Reference Example 95. (R)-8-methyl-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0662] Step 1: tert-butyl (R)-3-((8-methylquinolin-4-yl)amino)pyrrolidine-1-carboxylat e

[0663] The title compound (425.0 mg, 96.7 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 4-bromo-8-methylquinoline (298.1 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 328.2 (M + H)+

[0664] Step 2: (R)-8-methyl-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0665] The title compound (406.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0666] Reference Example 96. (R)-6-fluoro-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0667] Step 1: tert-butyl (R)-3-((6-fluoroquinolin-4-yl)amino)pyrrolidine-1-carboxylat e

[0668] The title compound (367.7.0 mg, 82.7 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 4-bromo-6-fluoro-quinoline (303.4 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 332.2 (M + H)+

[0669] Step 2: (R)-6-fluoro-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0670] The title compound (337.7 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0671] Reference Example 97. (R)-6-chloro-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0672] Step 1: tert-butyl (R)-3-((6-chloroquinolin-4-yl)amino)pyrrolidine-1-carboxylat e

[0673] The title compound (298.1 mg, 63.9 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 4-bromo-6-chloro-quinoline (325.5 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 348.1 (M + H)+

[0674] Step 2: (R)-6-chloro-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0675] The title compound (274.8 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0676] Reference Example 98. (R)-7-methoxy-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0677] Step 1: tert-butyl (R)-3-((7-methoxyquinolin-4-yl)amino)pyrrolidine-1-carboxyla te

[0678] The title compound (423.5 mg, 91.9 %) was prepared in the same fashion as Step 1 of Reference Example 17, except that 4-bromo-7-methoxy-quinoline (319.6 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 344.2 (M + H)+

[0679] Step 2: (R)-7-methoxy-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0680] The title compound (358.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0681] Reference Example 99. (R)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0682] Step 1: tert-butyl (R)-3-((8-fluoroquinolin-4-yl)amino)pyrrolidine-1-carboxylat e

[0683] The title compound (444.7 mg, 100.0 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 4-bromo-8-fluoroquinoline (303.4 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 332.2 (M + H)+

[0684] Step 2: (R)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0685] The title compound (418.0 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0686] Reference Example 100. (R)-8-methoxy-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0687] Step 1: tert-butyl (R)-3-((8-methoxyquinolin-4-yl)amino)pyrrolidine-1-carboxyla te

[0688] The title compound (348.5 mg, 75.6 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 4-bromo-8-methoxyquinoline (319.6 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 344.2 (M + H)+

[0689] Step 2: (R)-8-methoxy-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride

[0690] The title compound (316.4 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0691] Reference Example 101. (R)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-4-amine hydrochloride

[0692] Step 1: tert-butyl (R)-3-((8-(trifluoromethyl)quinolin-4-yl)amino)pyrrolidine-1 - carboxylate

[0693] The title compound (511.8 mg, 100.0 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 4-bromo-8-(trifluoromethyl)quinoline (370.5 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 382.2 (M + H)+

[0694] Step 2: (R)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-4-amine hydrochloride [0695] The title compound (499.4 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0696] Reference Example 102. (R)-3-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0697] Step 1: tert-butyl (R)-3-((3-methoxyquinolin-5-yl)amino)pyrrolidine-1-carboxyla te

[0698] The title compound (662.7 mg, 71.9 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 5 -bromo-3-methoxy quinoline (639.1 mg, 2.69 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 344.2 (M + H)+

[0699] Step 2: (R)-3-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0700] The title compound (610.3 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0701] Reference Example 103. (R)-2-chloro-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0702] Step 1: tert-butyl (R)-3-((2-chloroquinolin-6-yl)amino)pyrrolidine-1-carboxylat e

[0703] The title compound (720.5 mg, 77.2 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 2-chloro-6-bromoquinoline (651.0 mg, 2.69 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 348.2 (M + H)+

[0704] Step 2: (R)-2-chloro-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0705] The title compound (618.5 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0706] Reference Example 104. (R)-N-(pyrrolidin-3-yl)-2-(trifluoromethyl)quinolin-6-amine hydrochloride

[0707] Step 1: tert-butyl (R)-3-((2-(trifluoromethyl)quinolin-6-yl)amino)pyrrolidine-1 - carboxylate

[0708] The title compound (408.1 mg, 79.7 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 6-bromo-2-(trifluoromethyl)quinoline (370.5 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 382.2 (M + H)+

[0709] Step 2: (R)-N-(pyrrolidin-3-yl)-2-(trifluoromethyl)quinolin-6-amine hydrochloride

[0710] The title compound (379.0 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0711] Reference Example 105. (R)-3-methyl-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride [0712] Step 1: tert-butyl (R)-3-((3-methylquinolin-6-yl)amino)pyrrolidine-1-carboxylat e

[0713] The title compound (328.0 mg, 74.6 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 6-bromo-3-methylquinoline (298.1 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 328.2 (M + H)+

[0714] Step 2: (R)-3-methyl-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0715] The title compound (300.8 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0716] Reference Example 106. (R)-4-methoxy-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0717] Step 1: tert-butyl (R)-3-((4-methoxyquinolin-7-yl)amino)pyrrolidine-1-carboxyla te

[0718] The title compound (239.7 mg, 52.0 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 6-bromo-4-methoxy-quinoline (319.6 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 344.2 (M + H)+

[0719] Step 2: (R)-4-methoxy-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0720] The title compound (220.7 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0721] Reference Example 107. (R)-8-methyl-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0722] Step 1: tert-butyl (R)-3-((8-methylquinolin-6-yl)amino)pyrrolidine-1-carboxylat e

[0723] The title compound (258.6 mg, 58.8 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 6-bromo-8-methylquinoline (298.1 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 328.2 (M + H)+

[0724] Step 2: (R)-8-methyl-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0725] The title compound (238.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0726] Reference Example 108. (R)-8-chloro-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0727] Step 1: tert-butyl (R)-3-((8-chloroquinolin-6-yl)amino)pyrrolidine-1-carboxylat e

[0728] The title compound (311.9 mg, 66.8 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 6-bromo-8-chloro-quinoline (325.5 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 348.1 (M + H)+

[0729] Step 2: (R)-8-chloro-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride [0730] The title compound (287.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0731] Reference Example 109. (R)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-6-amine hydrochloride

[0732] Step 1: tert-butyl (R)-3-((8-(trifluoromethyl)quinolin-6-yl)amino)pyrrolidine-1 - carboxylate

[0733] The title compound (424.5 mg, 82.9 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 6-bromo-8-(trifluoromethyl)quinoline (370.5 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 382.1 (M + H)+

[0734] Step 2: (R)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-6-amine hydrochloride

[0735] The title compound (394.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0736] Reference Example 110. (R)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0737] Step 1: tert-butyl (R)-3-((8-fluoroquinolin-6-yl)amino)pyrrolidine-1-carboxylat e

[0738] The title compound (167.0 mg, 37.5 %) was prepared in the same fashion as Step 1 of

Reference Example 17, except that 6-bromo-8-fluoroquinoline (303.4 mg, 1.34 mmol) was used instead of 3-bromoquinoline. MS (ESI) m/z= 332.2 (M + H)+

[0739] Step 2: (R)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0740] The title compound (153.3 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0741] Reference Example 111. (S)-N-(pynOlidin-3-yl)quinolin-6-amine hydrochloride

[0742] Step 1: tert-butyl (S)-3-(quinolin-6-ylamino)pyrrolidin-1-carboxylate

[0743] The title compound (610.0 mg, 72.5 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 6-bromoquinoline (558.5 mg, 2.68 mmol) was used instead of 4- bromoquinoline. MS (ESI) m/z= 314.2 (M + H)+

[0744] Step 2: (S)-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0745] The title compound (102.0 mg, 61.3 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification. MS (ESI) m/z= 214.2 (M + H)+

[0746] Reference Example 112. (S)-5-(pyrrolidin-3-ylamino)quinolin-2-carbonitrile hydrochloride

[0747] Step 1: tert-butyl (S)-3-((2-cyanoquinolin-5-yl)amino)pyrrolidine-1-carboxylate

[0748] The title compound (818 mg, 90.0 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 5-bromoquinoline-2-carbonitrile (625.6 mg, 2.68 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 339.2 (M + H)+

[0749] Step 2: (S)-5-(pyrrolidin-3-ylamino)quinolin-2-carbonitrile hydrochloride

[0750] The title compound (200.2 mg, 66.5 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0751] Reference Example 113. (S)-5-(pyrrolidin-3-ylamino)quinolin-3-carbonitrile hydrochloride

[0752] Step 1: tert-butyl (S)-3-((3-cyanoquinolin-5-yl)amino)pyrrolidine-1-carboxylate

[0753] The title compound (698.0 mg, 76.8 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 5-bromoquinoline-3-carbonitrile (625.6 mg, 2.68 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 339.2 (M + H)+

[0754] Step 2: (S)-5-(pyrrolidin-3-ylamino)quinolin-3-carbonitrile hydrochloride

[0755] The title compound (143.2 mg, 47.9 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification

[0756] Reference Example 114. (S)-3-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0757] Step 1: tert-butyl (S)-3-((3-methoxyquinolin-5-yl)amino)pyrrolidine-1-carboxyla te

[0758] The title compound (446.2 mg, 48.4 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 5-bromo-3-methoxyquinoline (639.2 mg, 2.68 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 344.2 (M + H)+

[0759] Step 2: (S)-3-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0760] The title compound (187.5 mg, 59.3 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification

[0761] Reference Example 115. (S)-3-fluoro-N-(pynOlidin-3-yl)quinolin-5-amine hydrochloride

[0762] Step 1: tert-butyl (S)-3-((3-fluoroquinolin-5-yl)amino)pyrrolidine-1-carboxylat e

[0763] The title compound (668.7 mg, 75.1 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 5-bromo-3-fluoroquinoline (606.8 mg, 2.68 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 333.1 (M + H)+

[0764] Step 2: . (S)-3-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0765] The title compound (392.5 mg, 63.9 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification

[0766] Reference Example 116. (S)-3-chloro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0767] Step 1: tert-butyl (S)-3-((3-chloroquinolin-5-yl)amino)pyrrolidine-1-carboxylat e

[0768] The title compound (613.0 mg, 65.6 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 5-bromo-3-chloroquinoline (651.0 mg, 2.68 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 348.2 (M + H)+

[0769] Step 2: (S)-3-chloro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0770] The title compound (163.7 mg, 28.9 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification

[0771] Reference Example 117. (S)-7-fluoro-N-(pynOlidin-3-yl)quinolin-5-amine hydrochloride

[0772] Step 1: tert-butyl (S)-3-((7-fluoroquinolin-5-yl)amino)pyrrolidine-1-carboxylat e

[0773] The title compound (875.0 mg, 98.3 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 5-bromo-7-fluoroquinoline (606.8 mg, 2.68 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 333.1 (M + H)+

[0774] Step 2: (S)-7-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0775] The title compound (250.0 mg, 38.3 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification

[0776] Reference Example 118. (S)-7-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0777] Step 1: tert-butyl (S)-3-((7-methoxyquinolin-5-yl)amino)pyrrolidine-1-carboxyla te

[0778] The title compound (848.2 mg, 92.0 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 5-bromo-7-methoxyquinoline (639.2 mg, 2.68 mmol) was used instead of 4-bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.78 (d, 1H), 8.02 (d, 1H), 7.19 (dd, 1H), 6.89 (s, 1H), 6.28 (s, 1H), 4.43 (d, 1H), 4.15 (s, 1H), 3.93 (s, 3H), 3.79 (s, 1H), 3.56-3.36 (m, 3H), 2.27 (s, 1H), 2.05-1.93 (m, 1H), 1.47 (s, 9H).

[0779] Step 2: (S)-7-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0780] The title compound (485.0 mg, 62.0 %) was prepared in the same fashion as Step 2 of Reference Example 5 and used in the next step without any further purification

[0781] Reference Example 119. (S)-N-(pynOlidin-3-yl)-7-(trifluoromethyl)quinolin-5-amine hydrochloride

[0782] Step 1: tert-butyl (S)-3-((7-(trifluoromethyl)quinolin-5-yl)amino)pyrrolidine-1 - carboxylate

[0783] The title compound (888.5 mg, 86.7 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 5-bromo-7-(trifluoromethyl)quinoline (741.1 mg, 2.68 mmol) was used instead of 4-bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.97 (d, 1H), 8.19 (d, 1H), 7.80 (s, 1H), 7.47- 7.44 (m, 1H), 6.73 (s, 1H), 4.63 (s, 1H), 4.26 (s, 1H), 3.83 (s, 1H), 3.59-3.39 (m, 3H), 2.37- 2.29 (m, 1H) 2.07-2.05 (m, 1H), 1.49 (s, 9H).

[0784] Step 2: (S)-N-(pyrrolidin-3-yl)-7-(trifluoromethyl)quinolin-5-amine hydrochloride

[0785] The title compound (325.0 mg, 39.3 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification

[0786] Reference Example 120. (S)-6-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0787] Step 1: tert-butyl (S)-3-((6-methylquinolin-5-yl)amino)pyrrolidine-1-carboxylat e

[0788] The title compound (683.2 mg, 77.7 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 5-bromo-6-methylquinoline (596.2 mg, 2.68 mmol) was used instead of 4-bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.84 (d, 1H), 8.39 (d, 1H), 7.75 (d, 1H), 7.52 (d, 1H), 7.37 (d, 1H), 3.97 (s, 1H), 3.64-3.29 (m, 5H), 2.43 (s, 3H), 2.15-1.97 (m, 2H), 1.48-1.45 (s, 9H)

[0789] Step 2: (S)-6-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0790] The title compound (495.0 mg, 79.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification

[0791] Reference Example 121. (S)-6-methoxy-N-(pynOlidin-3-yl)quinolin-5-amine hydrochloride

[0792] Step 1: tert-butyl (S)-3-((6-methoxyquinolin-5-yl)amino)pyrrolidine-1-carboxyla te

[0793] The title compound (378.5 mg, 41.0 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 5-bromo-6-methoxyquinoline (639.2 mg, 2.68 mmol) was used instead of 4-bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.78 (s, 1H), 8.39 (d, 1H), 7.83 (d, 1H), 7.48 (d, 1H), 7.35-7.33 (m, 1H), 4.00-3.97 (m, 4H), 3.61-3.31 (m, 5H), 2.05-1.90 (m, 2H), 1.48-1.46 (s, 9H). [0794] Step 2: (S)-6-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0795] The title compound (248.5 mg, 71.2 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification

[0796] Reference Example 122. (S)-6-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0797] Step 1: tert-butyl (S)-3-((6-fluoroquinolin-5-yl)amino)pyrrolidine-1-carboxylat e

[0798] The title compound (344.7 mg, 38.7 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 5-bromo-6-fluoroquinoline (606.8 mg, 2.68 mmol) was used instead of 4-bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.88 (s, 1H), 8.37 (d, 1H), 7.75 (s, 1H), 7.49 (t, 1H), 7.41 (dd, 1H), 4.14 (d, 1H), 3.69-3.36 (m, 5H), 2.16-2.08 (m, 1H), 1.93 (s, 1H), 1.48- 1.46 (m, 9H).

[0799] Step 2: (S)-6-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0800] The title compound (251.7 mg, 79.5 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification

[0801] Reference Example 123. (S)-8-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0802] Step 1: tert-butyl (S)-3-((8-methylquinolin-5-yl)amino)pyrrolidine-1-carboxylat e

[0803] The title compound (631.0 mg, 71.7 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 5-bromo-8-methylquinoline (596.2 mg, 2.68 mmol) was used instead of 4-bromoquinoline. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.94 (d, 1H), 8.17 (d, 1H), 7.41 (s, 1H), 7.36 (dd, 1H), 6.60 (d, 1H), 4.19 (s, 2H), 3.83-3.78 (m, 1H), 3.56-3.48 (m, 2H), 3.40-3.32 (m, 1H), 2.69 (s, 3H), 2.27 (m, 1H), 2.04 (s, 1H), 1.47 (s, 9H).

[0804] Step 2: (S)-8-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0805] The title compound (489.3 mg, 84.5 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification

[0806] Reference Example 124. (S)-8-methoxy-N-(pynOlidin-3-yl)quinolin-5-amine hydrochloride

[0807] Step 1: tert-butyl (S)-3-((8-methoxyquinolin-5-yl)amino)pyrrolidine-1-carboxyla te

[0808] The title compound (712.0 mg, 77.2 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 5-bromo-8-methoxyquinoline (639.2 mg, 2.68 mmol) was used instead of 4-bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.94 (d, 1H), 8.20 (d, 1H), 7.42 (dd, 1H), 6.96 (s, 1H), 6.64 (d, 1H), 4.15-4.13 (m, 1H), 4.04 (s, 3H), 3.88-3.76 (m, 2H), 3.49-3.33 (m, 3H), 2.29-2.24 (m, 1H), 2.05-2.02 (m, 1H), 1.47 (s, 9H). [0809] Step 2: (S)-8-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0810] The title compound (505.5 mg, 77.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification

[0811] Reference Example 125. (S)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0812] Step 1: tert-butyl (S)-3-((8-fluoroquinolin-5-yl)amino)pyrrolidine-1-carboxylat e

[0813] The title compound (852.0 mg, 95.7 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 5-bromo-8-fluoroquinoline (606.8 mg, 2.68 mmol) was used instead of 4-bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.95 (d, 1H), 8.20 (d, 1H), 7.43 (dd, 1H), 7.29 (t, 1H), 6.55 (dd, 1H), 4.17 (s, 2H), 3.81 (s, 1H), 3.56-3.35 (m, 3H), 2.28 (s, 1H), 2.04 (s, 1H), 1.47 (s, 9H).

[0814] Step 2: (S)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0815] The title compound (556.6 mg, 71.2 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification

[0816] Reference Example 126. (S)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-5-amine hydrochloride

[0817] Step 1: tert-butyl (S)-3-((8-(trifluoromethyl)quinolin-5-yl)amino)pyrrolidine-1 - carboxylate

[0818] The title compound (318.0 mg, 31.0 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 5-bromo-8-(trifluoromethyl)quinoline (741.1 mg, 2.68 mmol) was used instead of 4-bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 9.04 (d, 1H), 8.16 (d, 1H), 7.92 (d, 1H), 7.43 (dd, 1H), 6.59 (d, 1H), 4.78 (s, 1H), 4.26 (s, 1H), 3.84 (s, 1H), 3.59-3.37 (m, 3H), 2.37-2.32 (m, 1H), 2.07-2.05 (m, 1H), 1.48 (s, 9H).

[0819] Step 2: (S)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-5-amine hydrochloride

[0820] The title compound (175.0 mg, 59.3 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification

[0821] Reference Example 127. (S)-N-(pyrrolidin-3-yl)-8-(trifluoromethoxy)quinolin-5-amine hydrochloride

[0822] Step 1: tert-butyl (S)-3-((8-(trifluoromethoxy)quinolin-5-yl)amino)pyrrolidine- 1- carboxylate

[0823] The title compound (844.5 mg, 79.1 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 5-bromo-8-(trifluoromethoxy)quinoline (784.0 mg, 2.68 mmol) was used instead of 4-bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.99 (d, 1H), 8.19 (d, 1H), 7.51 (d, 1H), 7.42 (dd, 1H), 6.56 (d, 1H), 4.50 (s, 1H), 4.19 (s, 1H), 3.81 (s, 1H), 3.56-3.36 (m, 2H), 2.35-2.25 (m, 1H), 2.05 (s, 1H), 1.48 (s, 9H).

[0824] Step 2: (S)-N-(pyrrolidin-3-yl)-8-(trifluoromethoxy)quinolin-5-amine hydrochloride

[0825] The title compound (505.3 mg, 64.2 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification

[0826] Reference Example 128. (S)-5-(pynOlidin-3-ylamino)quinoline-8-carbonitrile hydrochloride

[0827] Step 1: tert-butyl (S)-3-((8-cyanoquinolin-5-yl)amino)pyrrolidine-1-carboxylate

[0828] The title compound (800.0 mg, 88.0 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 5-bromoquinoline-8-carbonitrile (625.6 mg, 2.68 mmol) was used instead of 4-bromoquinoline. ¹ H-NMR (CDCI ₃ , 400MHz) δ 9.01 (d, 1H), 8.26 (d, 1H), 7.94 (d, 1H), 7.42 (dd, 1H), 6.60 (d, 1H), 5.30-5.25 (m, 1H), 4.27 (s, 1H), 3.83 (s, 1H), 3.57-3.40 (m, 3H), 2.38- 2.31 (m, 1H), 2.11 (s, 1H), 1.48 (s, 9H).

[0829] Step 2: (S)-5-(pyrrolidin-3-ylamino)quinoline-8-carbonitrile hydrochloride

[0830] The title compound (525.0 mg, 71.3 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification

[0831] Reference Example 129. (S)-8-(benzyloxy)-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0832] Step 1: tert-butyl (S)-3-((8-(benzyloxy)quinolin-5-yl)amino)pyrrolidine-1-carbo xylate

[0833] The title compound (1120.0 mg, 99.6 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 8-(benzyloxy)-5-bromoquinoline (843.4 mg, 2.68 mmol) was used instead of 4-bromoquinoline. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.97 (d, 1H), 8.19 (d, 1H), 7.50 (d, 2H), 7.41 (dd, 1H), 7.35 (t, 2H), 7.29 (d, 1H), 6.93 (d, 1H), 6.51 (d, 1H), 5.38 (s, 2H), 4.13-4.08 (m, 1H), 3.93 (s, 1H), 3.76-3.72 (m, 1H), 3.53-3.47 (m, 2H), 3.39-3.28 (m, 1H), 2.25-2.20 (m, 1H), 1.99 (s, 1H), 1.46 (s, 9H).

[0834] Step 2: (S)-8-(benzyloxy)-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0835] The title compound (1000.0 mg, 79.7 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification

[0836] Reference Example 130. (S)-N-(pyrrolidin-3-yl)quinolin-7-amine hydrochloride

[0837] Step 1: tert-butyl (S)-3-(quinolin-7-ylamino)pyrrolidin-1-carboxylate [0838] The title compound (631.5 mg, 75.0 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 7-bromoquinoline (558.5 mg, 2.68 mmol) was used instead of 4- bromoquinoline. MS (ESI) m/z= 314.2 (M + H)+

[0839] Step 2: (S)-N-(pyrrolidin-3-yl)quinolin-7-amine hydrochloride

[0840] The title compound (110.0 mg, 64.1 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification. MS (ESI) m/z= 214.2 (M + H)+

[0841] Reference Example 131. (S)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-4-amine hydrochloride

[0842] Step 1: tert-butyl (S)-3-((8-(trifluoromethyl)quinolin-4-yl)amino)pyrrolidin-1- carboxylate

[0843] The title compound (384.0 mg, 37.5 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 4-bromo-8-(trifluoromethyl)quinoline (741.0 mg, 2.68 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 382.2 (M + H)+

[0844] Step 2: (S)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-4-amine hydrochloride

[0845] The title compound (254.0 mg, 71.2 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification. MS (ESI) m/z= 282.2 (M + H)+

[0846] Reference Example 132. (S)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3-amine hydrochloride

[0847] Step 1: tert-butyl (S)-3-((1,8-naphthyridin-3-yl)amino)pyrrolidine-1-carboxylat e

[0848] The title compound (215.3 mg, 51.0 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 3-bromo-1,8-naphthyridine (280.6 mg, 1.34 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 315.1 (M + H)+

[0849] Step 2: (S)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3-amine hydrochloride

[0850] The title compound (196.7 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0851] Reference Example 133. (S)-7-chloro-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3-amine hydrochloride

[0852] Step 1: tert-butyl (S)-3-((7-chloro-1,8-naphthyridin-3-yl)amino)pyrrolidine-1-c arboxylate

[0853] The title compound (168.8 mg, 36.1 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 6-bromo-2-chloro-1,8-naphthyridine (326.8 mg, 1.34 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 349.2 (M + H)+ [0854] Step 2: (S)-7-chloro-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3-amine hydrochloride

[0855] The title compound (155.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0856] Reference Example 134. (S)-8-ethoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0857] Step 1: tert-butyl (S)-3-((8-ethoxyquinolin-5-yl)amino)pyrrolidin-1-carboxylate

[0858] The title compound (561.0 mg, 58.4 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 5-bromo-8-ethoxyquinoline (676.8 mg, 2.68 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 358.2 (M + H)+

[0859] Step 2: (S)-8-ethoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride

[0860] The title compound (321.0 mg, 61.9 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification. MS (ESI) m/z= 258.2 (M + H)+

[0861] Reference Example 135. (S)-5-(pynOlidin-3-ylamino)quinolin-8-ol hydrochloride

[0862] Step 1: 5-bromo-8-((tert-butyldimethylsilyl)oxy)quinoline

[0863] 5-Bromoquinolin-8-ol (1 g, 4.46 mmol) was dissolved in anhydrous dichloromethane. To the resulting solution, tert-butyldimethylsilyl chloride (0.74 g, 4.91 mmol), and imidazole (0.67 g, 9.819 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, the reaction mixture was was diluted with dichloromethane, and then added with water to extract an aqueous layer. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (n-hexane only) to give a title compound (1.16 g, 76.8 %). ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.88 (dd, 1H), 8.47 (dd, 1H), 7.68 (d, 1H), 7.49 (q, 1H), 7.07 (d, 1H), 1.07 (s, 9H), 0.27 (s, 6H)

[0864] Step 2: tert-butyl (S)-3-((8-((tert-butyldimethylsilyl)oxy)quinolin-5-yl)amino) pyrrolidine-

1-carboxylate

[0865] 5-Bromo-8-((tert-butyldimethylsilyl)oxy)quinoline (500 mg, 1.47 mmol) prepared in Step 1 was dissolved in anhydrous toluene. To the resulting solution, tert-butyl (S)-3-aminopyrrolidine-1- carboxylate (275.3 mg, 1.47 mmol), cesium carbonate (578 mg, 1.77 mmol), tris(dibenzylideneacetone)dipalladium(0) (162.4 mg, 0.17 mmol), and Ruphos (82.7mg, 0.17 mmol) were added. The reaction mixture was refluxed and stirred at 100 °C overnight. After the completion of the reaction, the reaction mixture was cooled to room temperature and then added with water to extract an aqueous layer with ethyl acetate. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 1/1, v/v) to give a title compound (655.3 mg,

99.9 %). MS (ESI) m/z= 444.3 (M + H)+

[0866] Step 3: (S)-5-(pyrrolidin-3-ylamino)quinolin-8-ol hydrochloride

[0867] tert-Butyl (S)-3-((8-((tert-butyldimethylsilyl)oxy)quinolin-5-yl)amino) pyrrolidine-1- carboxylate (655.3 mg, 1.47 mmol) prepared in Step 2 was dissolved in dichloromethane (8.4 ml) and then 4.0 M of hydrochloric acid dissolved in 1,4-dioxane was added thereto in excess drops. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, the solid compound obtained therefrom was filtered, washed, and vacuum dried to prepare a title compound (405.2 mg, 91.2 %). ¹ H-NMR ((CD ₃ ) ₂ SO, 400MHz) δ 9.55 (d, 1H), 8.99 (d, 1H), 7.98 (q, 1H), 7.43 (d, 1H), 6.96 (d, 1H), 4.48-4.46 (m, 1H), 3.67-3.61 (m, 2H), 3.53-3.47 (m, 2H), 2.53-2.44 (m, 1H), 2.36-2.29 (m, 1H).

[0868] Reference Example 136. (S)-8-methyl-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0869] Step 1: tert-butyl (S)-3-((8-methylquinolin-6-yl)amino)pyrrolidine-1-carboxylat e

[0870] The title compound (261.6 mg, 59.5 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 6-bromo-8-methylquinoline (298.1 mg, 1.34 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 328.2 (M + H)+

[0871] Step 2: (S)-8-methyl-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0872] The title compound (238.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0873] Reference Example 137. (S)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0874] Step 1: tert-butyl (S)-3-((8-fluoroquinolin-6-yl)amino)pyrrolidine-1-carboxylat e

[0875] The title compound (391.1 mg, 87.9 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 6-bromo-8-fluoroquinoline (303.4 mg, 1.34 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 332.2 (M + H)+

[0876] Step 2: (S)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0877] The title compound (294.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0878] Reference Example 138. (S)-8-chloro-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride

[0879] Step 1: tert-butyl (S)-3-((8-chloroquinolin-6-yl)amino)pyrrolidine-1-carboxylat e

[0880] The title compound (316.5 mg, 67.8 %) was prepared in the same fashion as Step 1 of Reference Example 5, except that 6-bromo-8-chloro-quinoline (325.5 mg, 1.34 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 348.1 (M + H)+

[0881] Step 2: (S)-8-chloro-N-(pyrrolidin-3-yl)quinobn-6-amine hydrochloride

[0882] The title compound (287.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0883] Reference Example 139. (S)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-6-amine hydrochloride

[0884] Step 1: tert-butyl (S)-3-((8-(trifluoromethyl)quinolin-6-yl)amino)pyrrolidine-1 - carboxylate

[0885] The title compound (232.4 mg, 45.4 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that 6-bromo-8-(trifluoromethyl)quinoline (370.5 mg, 1.34 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 382.1 (M + H)+

[0886] Step 2: (S)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-6-amine hydrochloride

[0887] The title compound (394.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification.

[0888] Reference Example 140. (S)-N-methyl-5-(pynOlidin-3-ylamino)quinoline-8-carboxamide hydrochloride

[0889] Step 1: 5-bromo-N-methylquinoline-8-carboxamide

[0890] 5-Bromoquinolin-8-carboxylic acid (300 mg, 1.19 mmol) was dissolved in dimethylformamide. To the resulting solution, methanamine (0.06 ml, 1.42 mmol), HATU (O-(7- azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluranium hexafluorophosphate) (633.6 mg, 1.67 mmol), and diisopropylethylamine (769.16 mg, 5.95 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 1/1, v/v) to give a title compound (229.8 mg, 72.8 %). ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.96 (dd, 1H), 8.74 (d, 1H), 8.72 (dd, 1H), 7.99 (d, 1H), 7.60 (q, 1H), 3.16 (d, 3H).

[0891] Step 2: tert-butyl (S)-3-((8-(methylcarbamoyl)quinolin-5-yl)amino)pyrrolidine-1 - carboxylate

[0892] 5-Bromo-N-methylquinoline-8-carboxamide (192 mg, 0.72 mmol) prepared in Step 1 was dissolved in anhydrous toluene. To the resulting solution, tert-butyl (S)-3-aminopyrrolidine-1- carboxylate (135.0 mg, 0.72 mmol), cesium carbonate (192.4 mg, 0.591 mmol), tris(dibenzylideneacetone)dipalladium(0) (49.2 mg, 0.05 mmol), and (+/-)-2,2'- bis(diphenylphospino)-1,1'-binaphthalene (40.1mg, 0.06 mmol) were added. The reaction mixture was refluxed and stirred at 100 °C overnight. After the completion of the reaction, the reaction mixture was cooled to room temperature and then added with water to extract an aqueous layer with ethyl acetate. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n- hexane= 1/1, v/v) to give a title compound (106.3 mg, 39.6 %). MS (ESI) m/z= 371.2 (M + H)+

[0893] Step 3: (S)-N-methyl-5-(pyrrolidin-3-ylamino)quinoline-8-carboxamide hydrochloride

[0894] tert-Butyl (S)-3-((8-(methylcarbamoyl)quinolin-5-yl)amino)pyrrolidine-1 -carboxylate

(106.3 mg, 0.287 mmol) prepared in Step 2 was dissolved in dichloromethane (8.4 ml) and then 4.0 M of hydrochloric acid dissolved in 1,4-dioxane was added thereto in excess drops. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, the solid compound obtained therefrom was filtered, washed, and vacuum dried to prepare a title compound (12.5 mg,

16.1 %). MS (ESI) m/z= 271.1 (M + H)+

[0895] Reference Example 141. (S)-N,N-dimethyl-5-(pyrrolidin-3-ylamino)quinoline-8- carboxamide hydrochloride

[0896] Step 1: 5-bromo-N,N-dimethylquinoline-8-carboxamide

[0897] The title compound (179.1 mg, 53.9 %) was prepared in the same fashion as Step 1 of

Reference Example 140, except that dimethylamine (64.1 mg, 1.42 mmol) was used instead of methanamine. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.95 (dd, 1H), 8.55 (dd, 1H), 7.86 (d, 1H), 7.56-7.52 (m, 2H), 3.24 (s, 3H), 2.77 (s, 3H).

[0898] Step 2: tert-butyl (S)-3-((8-(dimethylcarbamoyl)quinolin-5-yl)amino)pyrrolidine -1- carboxylate

[0899] The title compound (86.2 mg, 23.2 %) was prepared in the same fashion as Step 2 of

Reference Example 140. MS (ESI) m/z= 385.2 (M + H)+

[0900] Step 3: (S)-N,N-dimethyl-5-(pyrrolidin-3-ylamino)quinoline-8-carboxa mide hydrochloride

[0901] The title compound (57.0 mg, 71.4 %) was prepared in the same fashion as Step 3 of

Reference Example 140 and used in the next step without any further purification. MS (ESI) m/z=

285.1 (M + H)+

[0902] Reference Example 142. (S)-N-phenyl-5-(pyrrolidin-3-ylamino)quinoline-8-carboxamide hydrochloride

[0903] Step 1: 5-bromo-N-phenylquinoline-8-carboxamide

[0904] The title compound (348.12mg, 89.4 %) was prepared in the same fashion as Step 1 of

Reference Example 140, except that aniline (132.2 mg, 1.42 mmol) was used instead of methanamine. 1H-NMR (CDC13, 400MHz) δ 13.41 (s, 1H), 9.03 (d, 1H), 8.80 (d, 1H), 8.75 (d, 1H), 8.02 (d, 1H), 7.87 (d, 2H), 7.64 (q, 1H), 7.42 (t, 2H), 7.16 (t, 1H).

[0905] Step 2: tert-butyl (S)-3-((8-(pheny1carbamoyl)quinolin-5-yl)amino)pyrrolidine-1 - carboxylate

[0906] The title compound (54.0 mg, 38.3 %) was prepared in the same fashion as Step 2 of

Reference Example 140. MS (ESI) m/z= 433.2 (M + H)+

[0907] Step 3: (S)-N-phenyl-5-(pyrrolidin-3-ylamino)quinoline-8-carboxamide hydrochloride

[0908] The title compound (47.4 mg, 93.6 %) was prepared in the same fashion as Step 3 of

Reference Example 140 and used in the next step without any further purification. MS (ESI) m/z=

333.2 (M + H)+

[0909] Reference Example 143. (S)-N-methyl-N-phenyl-5-(pynOlidin-3-ylamino)quinoline-8- carboxamide hydrochloride

[0910] Step 1: 5-bromo-N-methyl-N-phenylquinoline-8-carboxamide

[0911] The title compound (365.2 mg, 89.9 %) was prepared in the same fashion as Step 1 of

Reference Example 140, except that N-methylaniline (152.2 mg, 1.42 mmol) was used instead of methanamine. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.95 (dd, 1H), 8.35 (dd, 1H), 7.56 (d, 1H), 7.41 (q, 1H), 7.30 (d, 1H), 7.05 (d, 2H), 6.97-6.91 (m, 3H), 3.59 (s, 3H)

[0912] Step 2: tert-butyl (S)-3-((8-(methyl(phenyl)carbamoyl)quinolin-5-yl)amino)pyrro lidine-1- carboxylate

[0913] The title compound (92.2 mg, 36.4 %) was prepared in the same fashion as Step 2 of

Reference Example 140. MS (ESI) m/z= 447.2 (M + H)+

[0914] Step 3: (S)-N-methyl-N-phenyl-5-(pyrrolidin-3-ylamino)quinoline-8-ca rboxamide hydrochloride

[0915] The title compound (85 mg, 98.5 %) was prepared in the same fashion as Step 3 of

Reference Example 140 and used in the next step without any further purification. MS (ESI) m/z=

347.2 (M + H)+

[0916] Reference Example 144. (S)-N-benzyl-N-methyl-5-(pyrrolidin-3-ylamino)quinoline-8- carboxamide hydrochloride

[0917] Step 1: N-benzyl-5-bromo-N-methylquinoline-8-carboxamide

[0918] The title compound (382.5 mg, 90.4 %) was prepared in the same fashion as Step 1 of

Reference Example 140, except that N-methyl-1-phenylmethanamine (172.0 mg, 1.42 mmol) was used instead of methanamine. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.99-8.98 (m, 1H), 8.54-8.49 (m, 1H), 7.86-7.78 (m, 1H), 7.59-7.50 (m, 3H), 7.41-7.37 (m, 1H), 7.31-7.19 (m, 3H), 4.93 (s, 1H), 4.29 (s, 1H), 3.15 (s, 1.5H), 2.65 (s, 1.5H)

[0919] Step 2: tert-butyl (S)-3-((8-(benzyl(methyl)carbamoyl)quinolin-5-yl)amino)pyrro lidine-1- carboxylate

[0920] The title compound (90.1 mg, 31.2 %) was prepared in the same fashion as Step 2 of

Reference Example 140. MS (ESI) m/z= 461.2 (M + H)+

[0921] Step 3: (S)-N-benzyl-N-methyl-5-(pyrrolidin-3-ylamino)quinoline-8-ca rboxamide hydrochloride

[0922] The title compound (76.0 mg, 89.2 %) was prepared in the same fashion as Step 3 of

Reference Example 140 and used in the next step without any further purification. MS (ESI) m/z= 361.2 (M + H)+

[0923] Reference Example 145. (S)-N-(pyridin-3-yl)-5-(pyrrolidin-3-ylamino)quinoline-8- carboxamide hydrochloride

[0924] Step 1: 5-bromo-N-(pyrid-3-yl)quinoline-8-carboxamide

[0925] The title compound (259.5 mg, 66.4 %) was prepared in the same fashion as Step 1 of

Reference Example 140, except that 3-aminopyridine (123.2 mg, 1.42 mmol) was used instead of methanamine. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 9.09 (dd, 1H), 8.88 (d, 1H), 8.83-8.79 (m, 2H), 8.51 (dt, 1H), 8.41 (dd, 1H), 8.07 (d, 1H), 7.70 (dd, 1H), 7.36 (dd, 1H).

[0926] Step 2: tert-butyl (S)-3-((8-(pyridin-3-y1carbamoyl)quinolin-5-yl)amino)pyrroli dine-1- carboxylate

[0927] The title compound (273.9 mg, 79.9 %) was prepared in the same fashion as Step 2 of

Reference Example 140. MS (ESI) m/z= 434.2 (M + H)+

[0928] Step 3: (S)-N-(pyridin-3-yl)-5-(pyrrolidin-3-ylamino)quinoline-8-car boxamide hydrochloride

[0929] The title compound (221.4 mg, 86.2 %) was prepared in the same fashion as Step 3 of

Reference Example 140 and used in the next step without any further purification. [0930] Reference Example 146. (S)-N-(pyridin-4-yl)-5-(pyrrolidin-3-ylamino)quinoline-8- carboxamide hydrochloride

[0931] Step 1: 5-bromo-N-(pyrid-4-yl)quinoline-8-carboxamide

[0932] The title compound (325.5 mg, 83.3 %) was prepared in the same fashion as Step 1 of

Reference Example 140, except that 4-aminopyridine (123.2 mg, 1.42 mmol) was used instead of methanamine. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 9.07 (dd, 1H), 8.81 (d, 2H), 8.59 (d, 2H), 8.07 (d, 1H), 7.79 (d, 2H), 7.71 (dd, 1H).

[0933] Step 2: tert-butyl (S)-3-((8-(pyridin-4-y1carbamoyl)quinolin-5-yl)amino)pyrroli dine-1- carboxylate

[0934] The title compound (363.6 mg, 84.5 %) was prepared in the same fashion as Step 2 of

Reference Example 140. MS (ESI) m/z= 434.2 (M + H)+

[0935] Step 3: (S)-N-(pyridin-4-yl)-5-(pyrrolidin-3-ylamino)quinoline-8-car boxamide hydrochloride

[0936] The title compound (330.0 mg, 96.7 %) was prepared in the same fashion as Step 3 of

Reference Example 140 and used in the next step without any further purification.

[0937] Reference Example 147. (S)-N-(pyridin-2-yl)-5-(pyrrolidin-3-ylamino)quinoline-8- carboxamide hydrochloride

[0938] Step 1: 5-bromo-N-(pyrid-2-yl)quinoline-8-carboxamide

[0939] The title compound (262.5 mg, 67.2 %) was prepared in the same fashion as Step 1 of

Reference Example 140, except that 2-aminopyridine (123.2 mg, 1.42 mmol) was used instead of methanamine. ¹ H-NMR (CDCI ₃ , 400MHz) δ 9.19 (dd, 1H), 8.82 (d, 1H), 8.77 (dd ,1H), 8.52 (d, 1H), 8.44 (dd, 1H), 8.05 (d, 1H), 7.78 (td, 1H), 7.68 (dd, 1H), 7.10 (dd, 1H).

[0940] Step 2: tert-butyl (S)-3-((8-(pyridin-2-y1carbamoyl)quinolin-5-yl)amino)pyrroli dine-1- carboxylate

[0941] The title compound (318.9 mg, 91.9 %) was prepared in the same fashion as Step 2 of

Reference Example 140. MS (ESI) m/z= 434.2 (M + H)+

[0942] Step 3: (S)-N-(pyridin-2-yl)-5-(pyrrolidin-3-ylamino)quinoline-8-car boxamide hydrochloride

[0943] The title compound (287.1 mg, 96.0 %) was prepared in the same fashion as Step 3 of

Reference Example 140 and used in the next step without any further purification.

[0944] Reference Example 148. (S)-N-(5-(pyrrolidin-3-ylamino)quinolin-8-yl)acetamide hydrochloride

[0945] Step 1: tert-butyl (S)-3-((8-acetamidoquinolin-5-yl)amino)pyrrolidin-1-carboxyl ate

[0946] The title compound (434.0 mg, 43.6 %) was prepared in the same fashion as Step 1 of

Reference Example 5, except that N-(5-bromoquinolin-8-yl)acetamide (711.7 mg, 2.68 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 371.2 (M + H)+

[0947] Step 2: (S)-N-(5-(pyrrolidin-3-ylamino)quinolin-8-yl)acetamide hydrochloride

[0948] The title compound (300.5 mg, 74.6 %) was prepared in the same fashion as Step 2 of

Reference Example 5 and used in the next step without any further purification. MS (ESI) m/z= 271.2 (M + H)+

[0949] Reference Example 149. (S)-N-(5-(pyrrolidin-3-ylamino)quinolin-8-yl)benzamide hydrochloride

[0950] Step 1: N-(5-bromo-quinolin-8-yl)benzamide

[0951] 5-Bromoquinolin-8-amine (500 mg, 2.24 mmol) was dissolved in dimethylformamide. To the resulting solution, benzoic acid (355.8 mg, 2.91 mmol), HATU (O-(7-azabenzotriazol-1-yl)- N,N,N',N'-tetramethyluranium hexafluorophosphate) (1278.4 mg, 8.96 mmol), and diisopropylethylamine (11158.83 mg, 8.96 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 1/1, v/v) to give a title compound (88.3 mg, 12.0 %). MS (ESI) m/z= 327.0 (M + H)+

[0952] Step 2: tert-butyl (S)-3-((8-(pheny1carbamoyl)quinolin-5-yl)amino)pyrrolidine-1 - carboxylate

[0953] The title compound (72.4 mg, 31.1 %) was prepared in the same fashion as Step 2 of

Reference Example 140 except that N-(5-bromo-quinolin-8-yl)benzamide (192 mg, 0.58 mmol) was used instead of 5-bromo-N-methylquinoline-8-carboxamide. MS (ESI) m/z= 433.2 (M + H)+

[0954] Step 3: (S)-N-(5-(pyrrolidin-3-ylamino)quinolin-8-yl)benzamide hydrochloride

[0955] The title compound (60.1 mg, 88.6 %) was prepared in the same fashion as Step 3 of

Reference Example 140 and used in the next step without any further purification. MS (ESI) m/z= 333.1 (M + H)+

[0956] Reference Example 150. (S)-6-methoxy-4-(pyrrolidin-3-yloxy)quinoline hydrochloride [0957] Step 1: tert-butyl (S)-3-((6-methoxyquinolin-4-yl)oxy)pyrrolidine-1-carboxylate

[0958] tert-Butyl (R)-3-hydroxypyrrolidine-1-carboxylate (187 mg, 1.0 mmol) was dissolved in tetrahydrofuran (5.0 mL). To the resulting solution, 4-hydroxy-6-methoxyquinoline (175 mg, 1.0 mmol) and triphenylphosphine (393 mg, 1.5 mmol) were added. The mixture solution was cooled to 0 °C. And then, DIAD (diisopropyl azodicarboxylate) (0.29 mL, 1.5 mmol) was slowly added dropwise for 15 minutes. The reaction mixture was stirred for 12 hours at room temperature. After the completion of the reaction, the reaction mixture was evaporated in vacuo and then purified with silica gel column chromatography (ethyl acetate/methanol= 10/1, v/v) to give a title compound (275 mg, 80%). MS (ESI) m/z= 345.2 (M + H)+

[0959] Step 2: (S)-6-methoxy-4-(pyrrolidin-3-yloxy)quinoline hydrochloride

[0960] tert-Butyl (S)-3-((6-methoxyquinolin-4-yl)oxy)pyrrolidine-1-carboxylate (275 mg, 0.80 mmol) prepared in Step 1 was dissolved in dichloromethane (2.4 mL) and methanol (0.2 mL). To the resulting solution, 4.0 M of hydrochloric acid dissolved in 1,4-dioxane was added in excess drops.

The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, the solid produced therefrom was filtered and dried to give a title compound (224 mg, 100%). The title compound was used in the next step without any further purification.

[0961] Reference Example 151. (S)-6,7-dimethoxy-4-(pyrrolidin-3-yloxy)quinoline hydrochloride

[0962] Step 1: tert-butyl (S)-3-((6,7-dimethoxyquinolin-4-yl)oxy)pyrrolidine-1-carboxy late

[0963] tert-Butyl (R)-3-hydroxypyrrolidine-1-carboxylate (187 mg, 1.0 mmol) was dissolved in tetrahydrofuran (5.0 mL). To the resulting solution, 4-hydroxy-6,7-dimethoxyquinoline (205 mg, 1.0 mmol) and triphenylphosphine (393 mg, 1.5 mmol) were added. The mixture solution was cooled to 0 °C. And then, DIAD (diisopropyl azodicarboxylate) (0.29 mL, 1.5 mmol) was slowly added dropwise for 15 minutes. The reaction mixture was stirred for 12 hours at room temperature. After the completion of the reaction, the reaction mixture was evaporated in vacuo and then purified with silica gel column chromatography (ethyl acetate/methanol= 10/1, v/v) to give a title compound (138 mg, 37%). MS (ESI) m/z= 375.2 (M + H)+

[0964] Step 2: (S)-6,7-dimethoxy-4-(pyrrolidin-3-yloxy)quinoline hydrochloride

[0965] tert-Butyl (S)-3-((6,7-dimethoxyquinolin-4-yl)oxy)pyrrolidine-1-carboxy late (138 mg,

0.37 mmol) prepared in Step 1 was dissolved in dichloromethane (1.0 mL) and methanol (0.1 mL). To the resulting solution, 4.0 M of hydrochloric acid dissolved in 1,4-dioxane was added in excess drops. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, the solid produced therefrom was filtered and dried to give a title compound (114 mg, 100%). The title compound was used in the next step without any further purification.

[0966] Reference Example 152. N-(piperidin-4-yl)quinoline-8-carboxamide hydrochloride

[0967] Step 1: tert-butyl 4-(quinoline-8-carboxamido)piperidine-1-carboxylate

[0968] 4-Amino- 1-tert-butoxycarbonylpiperidine (250.0 mg, 1.25 mmol) was dissolved in dichloromethane. To the resulting solution, 8-quinolinecarboxylic acid (216.2 mg, 1.25 mmol), 1- hydroxybenzotriazole hydrate (337.3 mg, 2.50 mmol), EDAC ( A-(3-di methylami nopropyl )-N'- ethy1carbodiimide hydrochloride) (478.6 mg, 2.50 mmol), and triethylamine (0.35 ml, 2.50 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 1/1, v/v) to give a title compound (443.6 mg, 99.9 %). MS (ESI) m/z= 356.2 (M + H)+

[0969] Step 2: N-(piperidin-4-yl)quinoline-8-carboxamide hydrochloride

[0970] tert-Butyl 4-(quinoline-8-carboxamido)piperidine-1-carboxylate (443.6 mg, 1.25 mmol) prepared in Step 1 was dissolved in dichloromethane (5.0 ml) and then 4.0 M of hydrochloric acid dissolved in 1,4-dioxane was added thereto in excess drops. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, the solid produced therefrom was filtered and dried to give a title compound (409.7 mg, 100.0 %). The title compound was used in the next step without any further purification.

[0971] Reference Example 153. N-ethyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[0972] Step 1: tert-butyl 4-(ethyl(quinolin-3-yl)amino)piperidine-1-carboxylate

[0973] tert-Butyl 4-(ethylamino)piperidine-1-carboxylate (109.7 mg, 0.48 mmol) was dissolved in anhydrous toluene. To the resulting solution, 3-bromoquinoline (100.0 mg, 0.48 mmol), cesium carbonate (187.9 mg, 0.58 mmol), tris(dibenzylideneacetone)dipalladium(0) (44.0 mg, 0.04 mmol), and (+/-)-2,2'-bis(diphenylphospino)-1,1'-binaphthalene (29.9 mg, 0.27 mmol) were added. The reaction mixture was refluxed and stirred at 100 °C overnight. After the completion of the reaction, the reaction mixture was cooled to room temperature and then added with water to extract an aqueous layer with ethyl acetate. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 1/1, v/v) to give a title compound (120.0 mg, 70.2 %). MS (ESI) m/z= 356.2 (M + H)+

[0974] Step 2: N-ethyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride [0975] tert-Butyl 4-(ethyl(quinolin-3-yl)amino)piperidine-1-carboxylate (120 mg, 0.34 mmol) prepared in Step 1 was dissolved in dichloromethane (2.2 ml) and then 4.0 M of hydrochloric acid dissolved in 1,4-dioxane was added thereto in excess drops. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, the solid compound obtained therefrom was filtered, washed, and vacuum dried to prepare a title compound (72.7 mg, 65.6 %) and used in the next step without any further purification.

[0976] Reference Example 154. N-ethyl-N-(piperidin-4-yl)quinolin-6-amine hydrochloride

[0977] Step 1: tert-butyl 4-(ethyl(quinolin-6-yl)amino)piperidine-1-carboxylate

[0978] The title compound (97.3 mg, 56.9 %) was prepared in the same fashion as Step 1 of

Reference Example 153, except that 6-bromoquinoline (100.0 mg, 0.48 mmol) was used instead of 3- bromoquinoline. MS (ESI) m/z= 356.2 (M + H)+

[0979] Step 2: N-ethyl-N-(piperidin-4-yl)quinolin-6-amine hydrochloride

[0980] The title compound (67.0 mg, 77.8 %) was prepared in the same fashion as Step 2 of

Reference Example 153 and used in the next step without any further purification.

[0981] Reference Example 155. N-ethyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[0982] Step 1: tert-butyl 4-(ethyl(quinolin-5-yl)amino)piperidine-1-carboxylate

[0983] The title compound (93.1 mg, 54.4 %) was prepared in the same fashion as Step 1 of

Reference Example 153, except that 5-bromoquinoline (100.0 mg, 0.48 mmol) was used instead of 3- bromoquinoline. MS (ESI) m/z= 356.2 (M + H)+

[0984] Step 2: N-ethyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[0985] The title compound (68.6 mg, 76.5 %) was prepared in the same fashion as Step 2 of

Reference Example 153 and used in the next step without any further purification.

[0986] Reference Example 156. N-methyl-N-(piperidin-4-yl)quinolin-2-amine hydrochloride

[0987] Step 1: tert-butyl 4-(methyl(quinolin-2-yl)amino)piperidine-1-carboxylate

[0988] tert-Butyl 4-(methylamino)piperidine-1-carboxylate (500.0 mg, 2.33 mmol) was dissolved in anhydrous toluene. To the resulting solution, 2-bromoquinoline (485.4 mg, 2.33 mmol), cesium carbonate (910. mg, 2.80 mmol), tris(dibenzylideneacetone)dipalladium(0) (210.0 mg, 0.23 mmol), and (+/-)-2,2'-bis(diphenylphospino)-1,1'-binaphthalene (170.0 mg, 0.28 mmol) were added. The reaction mixture was refluxed and stirred at 100 °C overnight. After the completion of the reaction, the reaction mixture was cooled to room temperature and then added with water to extract an aqueous layer with ethyl acetate. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 1/1, v/v) to give a title compound (203.8 mg, 25.5 %). MS (ESI) m/z= 342.3 (M + H)+

[0989] Step 2: N-methyl-N-(piperidin-4-yl)quinolin-2 -amine hydrochloride

[0990] tert-Butyl 4-(methyl(quinolin-2-yl)amino)piperidine-1-carboxylate (203.8 mg, 0.34 mmol) prepared in Step 1 was dissolved in dichloromethane (3.1 ml) and then 4.0 M of hydrochloric acid dissolved in 1,4-dioxane was added thereto in excess drops. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, the solid compound obtained therefrom was filtered, washed, and vacuum dried to prepare a title compound (128.8 mg, 68.6 %) and used in the next step without any further purification.

[0991] Reference Example 157. N-methyl-N-(piperidin-4-yl)quinolin-4-amine hydrochloride

[0992] Step 1: tert-butyl 4-(methyl(quinolin-4-yl)amino)piperidine-1-carboxylate

[0993] The title compound (85.2 mg, 15.5 %) was prepared in the same fashion as Step 1 of

Reference Example 156, except that 4-bromoquinoline (485.4 mg, 2.33 mmol) was used instead of 2- bromoquinoline. MS (ESI) m/z= 342.3 (M + H)+

[0994] Step 2: N-methyl-N-(piperidin-4-yl)quinolin-4-amine hydrochloride

[0995] The title compound (85.2 mg, 74.6 %) was prepared in the same fashion as Step 2 of

Reference Example 156 and used in the next step without any further purification.

[0996] Reference Example 158. N-methyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[0997] Step 1: tert-butyl 4-(methyl(quinolin-5-yl)amino)piperidine-1-carboxylate

[0998] The title compound (154.7 mg, 19.4 %) was prepared in the same fashion as Step 1 of

Reference Example 156, except that 5-bromoquinoline (485.4 mg, 2.33 mmol) was used instead of 2- bromoquinoline. MS (ESI) m/z= 342.3 (M + H)+

[0999] Step 2: N-methyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[1000] The title compound (141.0 mg, 99.1 %) was prepared in the same fashion as Step 2 of

Reference Example 156 and used in the next step without any further purification.

[1001] Reference Example 159. N-methyl-N-(piperidin-4-yl)quinolin-6-amine hydrochloride

[1002] Step 1: tert-butyl 4-(methyl(quinolin-6-yl)amino)piperidine-1-carboxylate

[1003] The title compound (184.2 mg, 23.1 %) was prepared in the same fashion as Step 1 of

Reference Example 156, except that 6-bromoquinoline (485.4 mg, 2.33 mmol) was used instead of 2- bromoquinoline. MS (ESI) m/z= 342.3 (M + H)+ [1004] Step 2: N-methyl-N-(piperidin-4-yl)quinolin-6-amine hydrochloride

[1005] The title compound (155.4 mg, 91.6 %) was prepared in the same fashion as Step 2 of

Reference Example 156 and used in the next step without any further purification.

[1006] Reference Example 160. N-methyl-N-(piperidin-4-yl)quinolin-7-amine hydrochloride

[1007] Step 1: tert-butyl 4-(methyl(quinolin-7-yl)amino)piperidine-1-carboxylate

[1008] The title compound (220.4 mg, 27.6 %) was prepared in the same fashion as Step 1 of

Reference Example 156, except that 7-bromoquinoline (485.4 mg, 2.33 mmol) was used instead of 2- bromoquinoline. MS (ESI) m/z= 342.3 (M + H)+

[1009] Step 2: N-methyl-N-(piperidin-4-yl)quinolin-7-amine hydrochloride

[1010] The title compound (198.0 mg, 97.6 %) was prepared in the same fashion as Step 2 of

Reference Example 156 and used in the next step without any further purification.

[1011] Reference Example 161. N-methyl-N-(piperidin-4-yl)furo[3,2-c]pyridin-7-amine hydrochloride

[1012] Step 1: tert-butyl 4-(furo[3,2-c]pyridin-7-yl(methyl)amino)piperidine-1-carboxy late

[1013] The title compound (230.8 mg, 29.8 %) was prepared in the same fashion as Step 1 of

Reference Example 156, except that 7-bromoftiro[3,2-c]pyridine (462.0 mg, 2.33 mmol) was used instead of 2-bromoquinoline. MS (ESI) m/z= 332.3 (M + H)+

[1014] Step 2: N-methyl-N-(piperidin-4-yl)furo[3,2-c]pyridin-7-amine hydrochloride

[1015] The title compound (153.7 mg, 72.5 %) was prepared in the same fashion as Step 2 of

Reference Example 156 and used in the next step without any further purification.

[1016] Reference Example 162. N-methyl-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride

[1017] Step 1: tert-butyl 4-(methyl(isoquinolin-5-yl)amino)piperidine-1-carboxy late

[1018] The title compound (228.0 mg, 28.6 %) was prepared in the same fashion as Step 1 of

Reference Example 156, except that 5-bromoisoquinoline (485.4 mg, 2.33 mmol) was used instead of 2-bromoquinoline. MS (ESI) m/z= 342.3 (M + H)+

[1019] Step 2: N-methyl-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride

[1020] The title compound (126.6 mg, 60.4 %) was prepared in the same fashion as Step 2 of

Reference Example 156 and used in the next step without any further purification.

[1021] Reference Example 163. N-methyl-N-(piperidin-4-yl)isoquinolin-6-amine hydrochloride

[1022] Step 1: tert-butyl 4-(methyl(isoquinolin-6-yl)amino)piperidine-1-carboxy late [1023] The title compound (165.0 mg, 20.7 %) was prepared in the same fashion as Step 1 of

Reference Example 156, except that 6-bromoisoquinoline (485.4 mg, 2.33 mmol) was used instead of 2-bromoquinoline. MS (ESI) m/z= 342.3 (M + H)+

[1024] Step 2: N-methyl-N-(piperidin-4-yl)isoquinolin-6-amine hydrochloride

[1025] The title compound (119.0 mg, 47.6 %) was prepared in the same fashion as Step 2 of

Reference Example 156 and used in the next step without any further purification.

[1026] Reference Example 164. N-methyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1027] Step 1: tert-butyl 4-(methyl(quinolin-3-yl)amino)piperidine-1-carboxylate

[1028] The title compound (124.0 mg, 15.5 %) was prepared in the same fashion as Step 1 of

Reference Example 156, except that 3-bromoquinoline (485.4 mg, 2.33 mmol) was used instead of 2- bromoquinoline. MS (ESI) m/z= 342.3 (M + H)+

[1029] Step 2: N-methyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1030] The title compound (69.5 mg, 60.9 %) was prepared in the same fashion as Step 2 of

Reference Example 156 and used in the next step without any further purification.

[1031] Reference Example 165. N-methyl-N-(piperidin-4-yl)isoquinolin-3-amine hydrochloride

[1032] Step 1: tert-butyl 4-(methyl(isoquinolin-3-yl)amino)piperidine-1-carboxy late

[1033] The title compound (406.5 mg, 51.0 %) was prepared in the same fashion as Step 1 of

Reference Example 156, except that 3-bromoisoquinoline (485.4 mg, 2.33 mmol) was used instead of 2-bromoquinoline. MS (ESI) m/z= 342.3 (M + H)+

[1034] Step 2: N-methyl-N-(piperidin-4-yl)isoquinolin-3-amine hydrochloride

[1035] The title compound (238.6 mg, 63.8 %) was prepared in the same fashion as Step 2 of

Reference Example 156 and used in the next step without any further purification.

[1036] Reference Example 166. N-methyl-N-(piperidin-4-yl)isoquinolin-7-amine hydrochloride

[1037] Step 1: tert-butyl 4-(methyl(isoquinolin-7-yl)amino)piperidine-1-carboxy late

[1038] The title compound (245.0 mg, 30.7 %) was prepared in the same fashion as Step 1 of

Reference Example 156, except that 7-bromoisoquinoline (485.4 mg, 2.33 mmol) was used instead of 2-bromoquinoline. MS (ESI) m/z= 342.3 (M + H)+

[1039] Step 2: N-methyl-N-(piperidin-4-yl)isoquinolin-7-amine hydrochloride

[1040] The title compound (132.7 mg, 58.3 %) was prepared in the same fashion as Step 2 of

Reference Example 156 and used in the next step without any further purification. [1041] Reference Example 167. N-methyl-N-(piperidm-4-yl)isoquinolin-8-amine hydrochloride

[1042] Step 1: tert-butyl 4-(methyl(isoquinolin-8-yl)amino)piperidine-1-carboxylate

[1043] The title compound (514.5 mg, 64.6 %) was prepared in the same fashion as Step 1 of

Reference Example 156, except that 8-bromoisoquinoline (485.4 mg, 2.33 mmol) was used instead of 2-bromoquinoline. MS (ESI) m/z= 342.3 (M + H)+

[1044] Step 2: N-methyl-N-(piperidin-4-yl)isoquinolin-8-amine hydrochloride

[1045] The title compound (303.3 mg, 64.1 %) was prepared in the same fashion as Step 2 of

Reference Example 156 and used in the next step without any further purification.

[1046] Reference Example 168. N, 6-dimethyl -N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1047] Step 1: tert-butyl 4-(methyl(6-methylquinolin-3-yl)amino)piperidine-1-carboxyla te

[1048] The title compound (750.3 mg, 45.2 %) was prepared in the same fashion as Step 1 of

Reference Example 156, except that 3-bromo-6-methylquinoline (518.1 mg, 2.33 mmol) was used instead of 2-bromoquinoline. MS (ESI) m/z= 356.2 (M + H)+

[1049] Step 2: N,6-dimethyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1050] The title compound (525.6 mg, 75.8 %) was prepared in the same fashion as Step 2 of

Reference Example 156 and used in the next step without any further purification. MS (ESI) m/z= 256.2 (M + H)+

[1051] Reference Example 169. 6-methoxy-N-methyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1052] Step 1: tert-butyl 4-(methyl(6-methyoxyquinolin-3-yl)amino)piperidine-1-carboxy late

[1053] The title compound (781.3 mg, 45.2 %) was prepared in the same fashion as Step 1 of

Reference Example 156, except that 3-bromo-6-methoxylquinoline (555.5 mg, 2.33 mmol) was used instead of 2-bromoquinoline. MS (ESI) m/z= 372.2 (M + H)+

[1054] Step 2: 6-methoxy-N-methyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1055] The title compound (420.3 mg, 57.8 %) was prepared in the same fashion as Step 2 of

Reference Example 156 and used in the next step without any further purification. MS (ESI) m/z= 272.1 (M + H)+

[1056] Reference Example 170. 7-methoxy-N-methyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1057] Step 1: tert-butyl 4-(methyl(7-methyoxyquinolin-3-yl)amino)piperidine-1-carboxy late [1058] The title compound (778.3 mg, 44.9 %) was prepared in the same fashion as Step 1 of

Reference Example 156, except that 3-bromo-7-methoxylquinoline (555.5 mg, 2.33 mmol) was used instead of 2-bromoquinoline. MS (ESI) m/z= 372.2 (M + H)+

[1059] Step 2: 7-methoxy-N-methyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1060] The title compound (303.3 mg, 41.7 %) was prepared in the same fashion as Step 2 of

Reference Example 156 and used in the next step without any further purification. MS (ESI) m/z= 272.2 (M + H)+

[1061] Reference Example 171. 8-methoxy-N-methyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1062] Step 1: tert-butyl 4-(methyl(8-methyoxyquinolin-3-yl)amino)piperidine-1-carboxy late

[1063] The title compound (780.0 mg, 45.0 %) was prepared in the same fashion as Step 1 of

Reference Example 156, except that 3-bromo-8-methoxylquinoline (555.5 mg, 2.33 mmol) was used instead of 2-bromoquinoline. MS (ESI) m/z= 372.2 (M + H)+

[1064] Step 2: 8-methoxy-N-methyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1065] The title compound (645.5 mg, 88.8 %) was prepared in the same fashion as Step 2 of

Reference Example 156 and used in the next step without any further purification. MS (ESI) m/z= 272.2 (M + H)+

[1066] Reference Example 172. 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[1067] Step 1: tert-butyl 4-((8-fluoroquinolin-5-yl)amino)piperidine-1-carboxylate

[1068] The title compound (163.9 mg, 38.0 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 5-bromo-8-fluoroquinoline (282.2 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 293.1 (M + H)+

[1069] Step 2: 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[1070] The title compound (151.0 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1071] Reference Example 173. 8-methyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[1072] Step 1: tert-butyl 4-((8-methylquinolin-5-yl)amino)piperidine-1-carboxylate

[1073] The title compound (66.5 mg, 15.6 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 5-bromo-8-methylquinoline (277.2 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 342.3 (M + H)+ [1074] Step 2: 8-methyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[1075] The title compound (61.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1076] Reference Example 174. 8-(benzyloxy)-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[1077] Step 1: tert-butyl 4-((8-(benzyloxy)quinolin-5-yl)amino)piperidine-1-carboxylat e

[1078] The title compound (143.2 mg, 26.5 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 8-(benzyloxy)-5-bromoquinoline (392.2 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 434.3 (M + H)+

[1079] Step 2: 8-(benzyloxy)-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[1080] The title compound (134.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1081] Reference Example 175. 3-chloro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[1082] Step 1: tert-butyl 4-((3-chloroquinolin-5-yl)amino)piperidine-1-carboxy late

[1083] The title compound (261.0 mg, 57.8 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 5-bromo-3-chloro-quinoline (302.7 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 262.1 (M + H)+

[1084] Step 2: 3-chloro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[1085] The title compound (241.4 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1086] Reference Example 176. N-(piperidin-4-yl)-8-(trifluoromethyl)quinolin-5-amine hydrochloride

[1087] Step 1: tert-butyl 4-((8-(trifluoromethyl)quinolin-5-yl)amino)piperidine-1-carb oxylate

[1088] The title compound (147.6 mg, 29.9 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 5-bromo-8-(trifluoromethyl)quinoline (344.6 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 396.2 (M + H)+

[1089] Step 2: N-(piperidin-4-yl)-8-(trifluoromethyl)quinolin-5-amine hydrochloride

[1090] The title compound (137.5 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1091] Reference Example 177. 5 -(piperidin-4-ylamino)quinoline-2-carbonitrile hydrochloride

[1092] Step 1: tert-butyl 4-((8-(trifluoromethyl)quinolin-5-yl)amino)piperidine-1-carb oxylate [1093] The title compound (324.8 mg, 73.8 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 5-bromoquinoline-2-carbonitrile (290.9 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 353.2 (M + H)+

[1094] Step 2: 5-(piperidin-4-ylamino)quinoline-2-carbonitrile hydrochloride

[1095] The title compound (299.7 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1096] Reference Example 178. N-(benzo[b]thiophen-4-yl)piperidin-4-amine hydrochloride

[1097] Step 1: tert-butyl 4-(benzo[b]thiophen-4-ylamino)piperidine-1-carboxylate

[1098] The title compound (220.4 mg, 53.1 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 4-bromobenzo[b]thiophene (266.0 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 333.1 (M + H)+

[1099] Step 2: N-(benzo[b]thiophen-4-yl)piperidin-4-amine hydrochloride

[1100] The title compound (220.4 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1101] Reference Example 179. 6-fluoro-N-(piperidin-4-yl)quinolin-4-amine hydrochloride

[1102] Step 1: tert-butyl 4-((6-fluoroquinolin-4-yl)amino)piperidine-1-carboxylate

[1103] The title compound (167.7 mg, 38.9 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 4-bromo-6-fluoroquinoline (282.2 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 346.2 (M + H)+

[1104] Step 2: 6-fluoro-N-(piperidin-4-yl)quinolin-4-amine hydrochloride

[1105] The title compound (154.5 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1106] Reference Example 180. 6-chloro-N-(piperidin-4-yl)quinolin-4-amine hydrochloride

[1107] Step 1: tert-butyl 4-((6-chloroquinolin-4-yl)amino)piperidine-1-carboxylate

[1108] The title compound (210.0 mg, 46.5 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 4-bromo-6-chloroquinoline (302.7 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 362.2 (M + H)+

[1109] Step 2: 6-chloro-N-(piperidin-4-yl)quinolin-4-amine hydrochloride

[1110] The title compound (194.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification. [1111] Reference Example 181. N-(piperidin-4-yl)isoquinolin-4-amine hydrochloride

[1112] Step 1: tert-butyl 4-(isoquinohn-4-ylamino)piperidine-1-carboxylate

[1113] The title compound (381.7 mg, 93.4 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 4-bromoisoquinoline (259.7 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 328.2 (M + H)+

[1114] Step 2: N-(piperidin-4-yl)isoquinolin-4-amine hydrochloride

[1115] The title compound (350.0 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1116] Reference Example 182. N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride

[1117] Step 1: tert-butyl 4-(isoquinolin-5-ylamino)piperidine-1-carboxylate

[1118] The title compound (333.6 mg, 81.6 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 5-bromoisoquinoline (259.7 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 328.2 (M + H)+

[1119] Step 2: N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride

[1120] The title compound (305.9 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1121] Reference Example 183. N-(piperidin-4-yl)isoquinolin-6-amine hydrochloride

[1122] Step 1: tert-butyl 4-(isoquinolin-6-ylamino)piperidine-1-carboxylate

[1123] The title compound (150.1 mg, 36.7 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 6-bromoisoquinoline (259.7 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 328.2 (M + H)+

[1124] Step 2: N-(piperidin-4-yl)isoquinolin-6-amine hydrochloride

[1125] The title compound (137.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1126] Reference Example 184. N-(piperidin-4-yl)isoquinolin-8-amine hydrochloride

[1127] Step 1: tert-butyl 4-(isoquinolin-8-ylamino)piperidine-1-carboxylate

[1128] The title compound (408.7 mg, 99.9 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 8-bromoisoquinoline (259.7 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 328.2 (M + H)+

[1129] Step 2: N-(piperidin-4-yl)isoquinolin-8-amine hydrochloride [1130] The title compound (374.8 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1131] Reference Example 185. 8-methyl-N-(piperidin-4-yl)quinolin-4-amine hydrochloride

[1132] Step 1: tert-butyl 4-((8-methylquinolin-4-yl)amino)piperidine-1-carboxylate

[1133] The title compound (336.4 mg, 78.9 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 4-bromo-8-methyl-quinoline (277.2 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 342.2 (M + H)+

[1134] Step 2: 8-methyl-N-(piperidin-4-yl)quinolin-4-amine hydrochloride

[1135] The title compound (309.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1136] Reference Example 186. 8-chloro-N-(piperidin-4-yl)quinolin-4-amine hydrochloride

[1137] Step 1: tert-butyl 4-((8-chloroquinolin-4-yl)amino)piperidine-1-carboxylate

[1138] The title compound (75.8 mg, 16.8 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 4-bromo-8-chloro-quinoline (302.7 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 362.2 (M + H)+

[1139] Step 2: 8-chloro-N-(piperidin-4-yl)quinolin-4-amine hydrochloride

[1140] The title compound (70.1 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1141] Reference Example 187. 8-fluoro-N-(piperidin-4-yl)quinolin-4-amine hydrochloride

[1142] Step 1: tert-butyl 4-((8-fluoroquinolin-4-yl)amino)piperidine-1-carboxylate

[1143] The title compound (209.4 mg, 48.6 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 4-bromo-8- fluoro-quinoline (282.2 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 346.2 (M + H)+

[1144] Step 2: 8-fluoro-N-(piperidin-4-yl)quinolin-4-amine hydrochloride

[1145] The title compound (192.9 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1146] Reference Example 188. N-(piperidin-4-yl)-8-(trifluoromethyl)quinolin-4-amine hydrochloride

[1147] Step 1: tert-butyl 4-((8-(trifluoromethyl)quinolin-4-yl)amino)piperidine-1-carb oxylate

[1148] The title compound (366.5 mg, 74.3 %) was prepared in the same fashion as Step 1 of Reference Example 28, except that 4-bromo-8-(trifluoromethyl)quinoline (344.6 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 396.2 (M + H)+

[1149] Step 2: N-(piperidin-4-yl)-8-(trifluoromethyl)quinolin-4-amine hydrochloride

[1150] The title compound (341.3 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1151] Reference Example 189. N-(piperidin-4-yl)-8-(trifluoromethoxy)quinolin-5-amine hydrochloride

[1152] Step 1: tert-butyl 4-((8-(trifluoromethoxy)quinolin-5-yl)amino)piperidine-1-car boxylate

[1153] The title compound (437.1 mg, 85.1 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 5-bromo-8-trifluoromethoxyquinoline (364.6 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 412.2 (M + H)+

[1154] Step 2: N-(piperidin-4-yl)-8-(trifluoromethoxy)quinolin-5-amine hydrochloride

[1155] The title compound (408.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1156] Reference Example 190. 3-methoxy-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[1157] Step 1: tert-butyl 4-((3-methoxyquinolin-5-yl)amino)piperidine-1-carboxylate

[1158] The title compound (327.6 mg, 73.4 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 5 -bromo-3-methoxy quinoline (297.2 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 358.2 (M + H)+

[1159] Step 2: 3-methoxy-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[1160] The title compound (302.7 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1161] Reference Example 191. 3-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[1162] Step 1: tert-butyl 4-((3-fluoroquinolin-5-yl)amino)piperidine-1-carboxylate

[1163] The title compound (343.7 mg, 79.7 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 5-bromo-3-fluoro-quinoline (282.2 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 346.2 (M + H)+

[1164] Step 2: 3-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[1165] The title compound (316.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification. [1166] Reference Example 192. 1-chloro-N-(piperidin-4-yl)isoquinolin-5 -amine hydrochloride

[1167] Step 1: tert-butyl 4-((1-chloroisoquinolin-5-yl)amino)piperidine-1-carboxylate

[1168] The title compound (49.5 mg, 11.0 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 5-bromo-1-chloro-isoquinoline (302.7 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 362.2 (M + H)+

[1169] Step 2: 1-chloro-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride

[1170] The title compound (45.8 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1171] Reference Example 193. 3-chloro-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride

[1172] Step 1: tert-butyl 4-((3-chloroisoquinolin-5-yl)amino)piperidine-1-carboxylate

[1173] The title compound (332.2 mg, 73.5 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 5-bromo-3-chloro-isoquinoline (302.7 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 362.2 (M + H)+

[1174] Step 2: 3-chloro-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride

[1175] The title compound (307.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1176] Reference Example 194. 8-chloro-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride

[1177] Step 1: tert-butyl 4-((8-chloroisoquinolin-5-yl)amino)piperidine-1-carboxylate

[1178] The title compound (265.1 mg, 58.7 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 5-bromo-8-chloro-isoquinoline (302.7 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 362.2 (M + H)+

[1179] Step 2: 8-chloro-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride

[1180] The title compound (245.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1181] Reference Example 195. 4-methyl-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride

[1182] Step 1: tert-butyl 4-((4-methylisoquinolin-5-yl)amino)piperidine-1-carboxylate

[1183] The title compound (284.3 mg, 66.7 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 5-bromo-4-methyl-isoquinoline (277.2 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 342.2 (M + H)+

[1184] Step 2: 4-methyl-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride [1185] The title compound (261.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1186] Reference Example 196. 3-methoxy-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride

[1187] Step 1: tert-butyl 4-((3-methoxyisoquinolin-5-yl)amino)piperidine-1-carboxylate

[1188] The title compound (407.0 mg, 91.2 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 5-bromo-3-methoxy-isoquinoline (297.2 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 358.2 (M + H)+

[1189] Step 2: 3-methoxy-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride

[1190] The title compound (376.0 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1191] Reference Example 197. 8-nitro-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride

[1192] Step 1: tert-butyl 4-((8-nitroisoquinolin-5-yl)amino)piperidine-1-carboxylate

[1193] The title compound (178.1 mg, 38.2 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 5-bromo-8-nitro-isoquinoline (315.9 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 373.2 (M + H)+

[1194] Step 2: 8-nitro-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride

[1195] The title compound (165.1 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1196] Reference Example 198. 8-fluoro-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride

[1197] Step 1: tert-butyl 4-((8-fluoroisoquinolin-5-yl)amino)piperidine-1-carboxylate

[1198] The title compound (377.9 mg, 87.7 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 5-bromo-8-fluoro-isoquinoline (282.2 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 346.2 (M + H)+

[1199] Step 2: 8-fluoro-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride

[1200] The title compound (348.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1201] Reference Example 199. 2-chloro-6,7-dimethoxy-N-(piperidin-4-yl)quinazolin-4-amine hydrochloride

[1202] Step 1: tert-butyl 4-((2-chloro-6,7-dimethoxyquinazolin-4-yl)amino)piperidine-1 - carboxylate [1203] tert-Butyl 4-aminopiperidine-1-carboxylate (250.0 mg, 1.25 mmol) was dissolved in anhydrous dichloromethane. To the resulting solution, 2,4-dichloro-6,7-dimethoxyquinazoline (291.1 mg, 1.12 mmol), and triethylamine (0.35 ml, 2.50 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, saturated NH ₄ CI aqueous solution was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 1/1, v/v) to give a title compound (204.0 mg, 38.6 %). MS (ESI) m/z= 423.2 (M + H)+

[1204] Step 2: 2-chloro-6,7-dimethoxy-N-(piperidin-4-yl)quinazolin-4-amine hydrochloride

[1205] tert-Butyl 4-((2-chloro-6,7-dimethoxyquinazolin-4-yl)amino)piperidine-1 -carboxylate

(204.0 mg, 0.48 mmol) prepared in Step 1 was dissolved in dichloromethane (5.0 ml). To the resulting solution, 4.0 M of hydrochloric acid dissolved in 1,4-dioxane was added in excess drops. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, the solid produced therefrom was filtered and dried to give a title compound (190.9 mg, 100.0 %). The title compound was used in the next step without any further purification.

[1206] Reference Example 200. 2-chloro-8-methyl-N-(piperidin-4-yl)quinazolin-4-amine hydrochloride

[1207] Step 1: tert-butyl 4-((2-chloro-8-methylquinazolin-4-yl)amino)piperidine-1-carb oxylate

[1208] The title compound (138.7 mg, 29.5 %) was prepared in the same fashion as Step 1 of

Reference Example 199, except that 2,4-dichloro-8-methylquinazoline (239.4 mg, 1.25 mmol) was used instead of 2,4-dichloro-6,7-dimethoxyquinazoline. MS (ESI) m/z= 377.2 (M + H)+

[1209] Step 2: 2-chloro-8-methyl-N-(piperidin-4-yl)quinazolin-4-amine hydrochloride

[1210] The title compound (128.7 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 199 and used in the next step without any further purification.

[1211] Reference Example 201. N-(piperidin-4-yl)-2,6-naphthyridin-4-amine hydrochloride

[1212] Step 1: tert-butyl 4-((2,6-naphthyridin-4-yl)amino)piperidine-1-carboxylate

[1213] The title compound (113.5 mg, 27.7 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 4-bromo-2,6-naphthyridine (260.9 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 329.2 (M + H)+

[1214] Step 2: N-(piperidin-4-yl)-2,6-naphthyridin-4-amine hydrochloride

[1215] The title compound (104.1 mg, 100.0 %) was prepared in the same fashion as Step 2 of Reference Example 28 and used in the next step without any further purification.

[1216] Reference Example 202. 8-chloro-N-(piperidin-4-yl)isoquinolin-4-amine hydrochloride

[1217] Step 1: tert-butyl 4-((8-chloroisoquinolin-4-yl)amino)piperidine-1-carboxylate

[1218] The title compound (102.8 mg, 22.8 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 4-bromo-8-chloro-isoquinoline (302.7 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 362.2 (M + H)+

[1219] Step 2: 8-chloro-N-(piperidin-4-yl)isoquinolin-4-amine hydrochloride

[1220] The title compound (95.1 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1221] Reference Example 203. N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1222] Step 1: tert-butyl 4-(quinolin-3-ylamino)piperidine-1-carboxylate

[1223] The title compound (212.5 mg, 99.9 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 3-bromoquinoline (135.0 mg, 0.65 mmol) was used instead of 4- bromoquinoline. MS (ESI) m/z= 328.2 (M + H)+

[1224] Step 2: N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1225] The title compound (202.9 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1226] Reference Example 204. 6-methyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1227] Step 1: tert-butyl 4-((6-methylquinolin-3-yl)amino)piperidine-1-carboxylate

[1228] The title compound (143.3 mg, 64.7 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 3-bromo-6-methylquinoline (144.2 mg, 0.65 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 342.3 (M + H)+

[1229] Step 2: 6-methyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1230] The title compound (131.9 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1231] Reference Example 205. 6-fluoro-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1232] Step 1: tert-butyl 4-((6-fluoroquinolin-3-yl)amino)piperidine-1-carboxy late

[1233] The title compound (218.3 mg, 64.7 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 3-bromo-6-fluoroquinoline (146.7 mg, 0.65 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 346.2 (M + H)+ [1234] Step 2: 6-fluoro-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1235] The title compound (201.1 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1236] Reference Example 206. 6-methoxy-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1237] Step 1: tert-butyl 4-((6-methoxyquinolin-3-yl)amino)piperidine-1-carboxylate

[1238] The title compound (194.2 mg, 83.7 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 3 -bromo-6-methoxy quinoline (154.5 mg, 0.65 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 358.2 (M + H)+

[1239] Step 2: 6-methoxy-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1240] The title compound (179.4 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1241] Reference Example 207. 7-methoxy-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1242] Step 1: tert-butyl 4-((7-methoxyquinolin-3-yl)amino)piperidine-1-carboxylate

[1243] The title compound (66.0 mg, 28.5 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 3 -bromo-7-methoxy quinoline (154.5 mg, 0.65 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 358.2 (M + H)+

[1244] Step 2: 7-methoxy-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1245] The title compound (61.0 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1246] Reference Example 208. 8-methoxy-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1247] Step 1: tert-butyl 4-((8-methoxyquinolin-3-yl)amino)piperidine-1-carboxylate

[1248] The title compound (78.5 mg, 33.8 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 3-bromo-8-methoxyquinoline (154.5 mg, 0.65 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 358.1 (M + H)+

[1249] Step 2: 8-methoxy-N-(piperidin-4-yl)quinolin-3-amine hydrochloride

[1250] The title compound (72.5 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1251] Reference Example 209. N-(piperidin-4-yl)-1,8-naphthyridin-3-amine hydrochloride

[1252] Step 1: tert-butyl 4-((1,8-naphthyridin-3-yl)amino)piperidine-1-carboxylate [1253] The title compound (189.1 mg, 46.1 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 3-bromo-1,8-naphthyridine (260.9 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 329.2 (M + H)+

[1254] Step 2: N-(piperidin-4-yl)-1,8-naphthyridin-3-amine hydrochloride

[1255] The title compound (173.4 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1256] Reference Example 210. 7-chloro-N-(piperidin-4-yl)-1,8-naphthyridin-3-amine hydrochloride

[1257] Step 1: tert-butyl 4-((7-chloro-1,8-naphthyridin-3-yl)amino)piperidine-1-carbox ylate

[1258] The title compound (57.9 mg, 12.8 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 6-bromo-2-chloro-1,8-naphthyridine (303.9 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 363.2 (M + H)+

[1259] Step 2: 7-chloro-N-(piperidin-4-yl)-1,8-naphthyridin-3-amine hydrochloride

[1260] The title compound (53.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1261] Reference Example 211. 8-ethoxy-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[1262] Step 1: tert-butyl 4-((8-ethoxyquinolin-5-yl)amino)piperidine-1-carboxylate

[1263] The title compound (776.4 mg, 83.7 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 5-bromo-8-ethoxyquinoline (629.4 mg, 2.49 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 372.2 (M + H)+

[1264] Step 2: 8-ethoxy-N-(piperidin-4-yl)quinolin-5-amine hydrochloride

[1265] The title compound (432.6 mg, 60.1 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification. MS (ESI) m/z= 272.2 (M + H)+

[1266] Reference Example 212. N-(piperidin-4-yl)benzo[d]thiazol-7-amine hydrochloride

[1267] Step 1: tert-butyl 4-(1,3-benzothiazol-7-ylamino)piperidine-1-carboxylate

[1268] 1-Boc-4-piperidone (250.0 mg, 1.26 mmol) was dissolved in anhydrous dichloromethane. To the resulting solution, benzo[d]thiazol-7-amine (188.5 mg, 1.26 mmol), acetic acid (0.15 ml), and sodium triacetoxyborohydride (265.9 mg, 1.26 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, saturated sodium carbonate aqueous solution was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 1/1, v/v) to give a title compound (375.3 mg, 89.7 %). MS (ESI) m/z= 334.2 (M + H)+

[1269] Step 2: N-(piperidin-4-yl)benzo[d]thiazol-7-amine hydrochloride

[1270] tert-butyl 4-(1,3-benzothiazol-7-ylamino)piperidine-1-carboxylate (375.3 mg, 1.13 mmol) prepared in Step 1 was dissolved in dichloromethane (5.0 ml). To the resulting solution, 4.0 M of hydrochloric acid dissolved in 1,4-dioxane was added in excess drops. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, the solid produced therefrom was filtered and dried to give a title compound (344.8 mg, 100.0 %). The title compound was used in the next step without any further purification.

[1271] Reference Example 213. 1-(4-(piperidin-4-ylamino)indolin-1-yl)ethan-1-one hydrochloride

[1272] Step 1: tert-butyl 4-[(1-acetylindolin-4-yl)amino]piperidine-1-carboxylate

[1273] The title compound (310.2 mg, 68.8 %) was prepared in the same fashion as Step 1 of

Reference Example 212, except that 1-(4-aminoindolin-1-yl)ethanone (221.1 mg, 1.26 mmol) was used instead of benzo[d]thiazol-7-amine. MS (ESI) m/z= 360.2 (M + H)+

[1274] Step 2: 1-(4-(piperidin-4-ylamino)indolin-1-yl)ethan-1-one hydrochloride

[1275] The title compound (286.8 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 212 and used in the next step without any further purification.

[1276] Reference Example 214. 8-methyl-N-(piperidin-4-yl)quinolin-6-amine hydrochloride

[1277] Step 1: tert-butyl 4-((8-methylquinolin-6-yl)amino)piperidine-1-carboxylate

[1278] The title compound (196.4 mg, 50.1 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 6-bromo-8-methylquinoline (255.0 mg, 1.15 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 342.2 (M + H)+

[1279] Step 2: 8-methyl-N-(piperidin-4-yl)quinolin-6-amine hydrochloride

[1280] The title compound (180.7 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1281] Reference Example 215. 8-fluoro-N-(piperidin-4-yl)quinolin-6-amine hydrochloride

[1282] Step 1: tert-butyl 4-((8-fluoroquinolin-6-yl)amino)piperidine-1-carboxylate

[1283] The title compound (317.3 mg, 80.0 %) was prepared in the same fashion as Step 1 of Reference Example 28, except that 6-bromo-8-fluoroquinoline (259.6 mg, 1.15 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 346.2 (M + H)+

[1284] Step 2: 8-fluoro-N-(piperidin-4-yl)quinolin-6-amine hydrochloride

[1285] The title compound (292.4 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1286] Reference Example 216. 8-chloro-N-(piperidin-4-yl)quinolin-6-amine hydrochloride

[1287] Step 1: tert-butyl 4-((8-chloroquinolin-6-yl)amino)piperidine-1-carboxylate

[1288] The title compound (369.5 mg, 88.9 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 6-bromo-8-chloro-quinoline (278.5 mg, 1.15 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 362.2 (M + H)+

[1289] Step 2: 8-chloro-N-(piperidin-4-yl)quinolin-6-amine hydrochloride

[1290] The title compound (341.7 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1291] Reference Example 217. N-(piperidin-4-yl)-8-(trifluoromethyl)quinolin-6-amine hydrochloride

[1292] Step 1: tert-butyl 4-((8-(trifluoromethyl)quinolin-6-yl)amino)piperidine-1-carb oxylate

[1293] The title compound (284.3 mg, 62.6 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 6-bromo-8-(trifluoromethyl)quinoline (317.0 mg, 1.15 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 396.2 (M + H)+

[1294] Step 2: N-(piperidin-4-yl)-8-(trifluoromethyl)quinolin-6-amine hydrochloride

[1295] The title compound (264.8 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1296] Reference Example 218. 4-(piperidin-4-ylamino)-2H-chromen-2-one hydrochloride

[1297] Step 1: tert-butyl 4-((2-oxo-2H-chromen-4-yl)amino)piperidine-1-carboxylate

[1298] Tert-butyl 4-aminopiperidine-1-carboxylate (300 mg, 1.4 mmol) was dissolved in anhydrous DMF. To the resulting solution, 4-bromo-2H-chromen-2-one (337.1 mg, 1.49 mmol) and potassium carbonate (414.0 mg, 2.996 mmol) were added. The reaction mixture was stirred at 100 °C overnight. After the completion of the reaction, the reaction mixture was cooled to room temperature and then added with water to extract an aqueous layer with ethyl acetate. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 1/1, v/v) to give a title compound (300 mg, 58.1 %). ¹ H-NMR (MeOD ₄ , 400MHz) δ 8.01 (dd, 1H), 7.61 (td, 1H), 7.36-7.32 (m, 2H), 5.41 (s, 1H), 4.16 (d, 2H), 3.77-3.75 (m, 1H), 2.97 (s, 2H), 2.07-2.02 (m, 2H), 1.60-1.53 (m, 2H), 1.49 (s, 9H).

[1299] Step 2: 4-(piperidin-4-ylamino)-2H-chromen-2-one hydrochloride

[1300] The title compound (210.0 mg, 76.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification. ¹ H-NMR (McOD ₄ , 400MHz) δ 8.05 (dd, 1H), 7.63 (td, 1H), 7.38-7.33 (m, 2H), 5.45 (s, 1H), 3.95-3.89 (m, 1H), 3.53 (d, 2H), 3.22 (td, 2H), 2.33 (d, 2H), 2.00-1.89 (m, 2H).

[1301] Reference Example 219. N-(piperidin-4-yl)-1,5-naphthyridin-4-aminehydrochloride

[1302] Step 1: tert-butyl 4-((1,5-naphthyridin-4-yl)amino)piperidine-1-carboxylate

[1303] The title compound (700.0 mg, 85.4 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 4-bromo-1,5-naphthyridine (521.8 mg, 2.49 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 329.2 (M + H)+

[1304] Step 2: N-(piperidin-4-yl)-1,5-naphthyridin-4-aminehydrochloride

[1305] The title compound (321.0 mg, 80.6 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification. MS (ESI) m/z= 229.2 (M + H)+

[1306] Reference Example 220. N-(piperidin-4-yl)-2-(trifluoromethyl)quinolin-6-amine hydrochloride

[1307] Step 1: tert-butyl 4-((2-(trifluoromethyl)quinolin-6-yl)amino)piperidine-1-carb oxylate

[1308] The title compound (462.5 mg, 93.7 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 6-bromo-2-(trifluoromethyl)quinoline (344.6 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 396.2 (M + H)+

[1309] Step 2: N-(piperidin-4-yl)-2-(trifluoromethyl)quinolin-6-amine hydrochloride

[1310] The title compound (430.8 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1311] Reference Example 221. 4-methoxy-N-(piperidin-4-yl)quinolin-6-amine hydrochloride

[1312] Step 1: tert-butyl 4-((4-methoxyquinolin-6-yl)amino)piperidine-1-carboxylate

[1313] The title compound (110.2 mg, 24.7 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 6-bromo-4-methoxy-quinoline (297.2 mg, 1.25 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 358.2 (M + H)+ [1314] Step 2: 4-methoxy-N-(piperidin-4-yl)quinolin-6-amine hydrochloride

[1315] The title compound (101.7 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1316] Reference Example 222. 2-bromo-N-(piperidin-4-yl)pyridin-4-amine hydrochloride

[1317] Step 1: tert-butyl 4-[(2-bromo-4-pyridyl)amino]piperidine-1-carboxylate

[1318] The title compound (257.0 mg, 57.5 %) was prepared in the same fashion as Step 1 of

Reference Example 212, except that 4-amino-2-bromopyridine (217.1 mg, 1.26 mmol) was used instead of benzo[d]thiazol-7-amine. MS (ESI) m/z= 358.1 (M + H)+

[1319] Step 2: 2-bromo-N-(piperidin-4-yl)pyridin-4-amine hydrochloride

[1320] The title compound (237.3 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 212 and used in the next step without any further purification.

[1321] Reference Example 223. N-(piperidin-4-yl)-1H-benzo[d]imidazol-7-amine hydrochloride

[1322] Step 1: tert-butyl 4-((1H-benzo[d]imidazol-7-yl)amino)piperidine-1-carboxylate

[1323] The title compound (319.6 mg, 80.5 %) was prepared in the same fashion as Step 1 of

Reference Example 212, except that 3H-benzo[d]imidazol-4-amine (167.1 mg, 1.26 mmol) was used instead of benzo[d]thiazol-7-amine. MS (ESI) m/z= 317.2 (M + H)+

[1324] Step 2: N-(piperidin-4-yl)-1H-benzo[d]imidazol-7-amine hydrochloride

[1325] The title compound (292.1 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 212 and used in the next step without any further purification.

[1326] Reference Example 224. 2-chloro-6-morpholino-N-(piperidin-4-yl)pyridin-4-amine hydrochloride

[1327] Step 1: tert-butyl 4-((2-chloro-6-morpholinopyridin-4-yl)amino)piperidine-1-car boxylate

[1328] The title compound (463.1 mg, 93.0 %) was prepared in the same fashion as Step 1 of

Reference Example 212, except that 4-amino-2-chloro-6-morpholinopyridine (268.1 mg, 1.26 mmol) was used instead of benzo[d]thiazol-7-amine. MS (ESI) m/z= 397.2 (M + H)+

[1329] Step 2: 2-chloro-6-morpholino-N-(piperidin-4-yl)pyridin-4-amine hydrochloride

[1330] The title compound (431.5 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 212 and used in the next step without any further purification.

[1331] Reference Example 225. N-(piperidin-4-yl)-3-(trifluoromethyl)quinolin-5-amine hydrochloride [1332] Step 1: tert-butyl 4-((3-(trifluoromethyl)quinolin-5-yl)amino)piperidine-1-carb oxylate

[1333] The title compound (853.9 mg, 86.5 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that 5-bromo-3-(trifluoromethyl)quinoline (689.2 mg, 2.50 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 396.1 (M + H)+

[1334] Step 2: N-(piperidin-4-yl)-3-(trifluoromethyl)quinolin-5-amine hydrochloride

[1335] The title compound (795.0 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification.

[1336] Reference Example 226. N-phenyl-5-(piperidin-4-ylamino)quinoline-3-carboxamide hydrochloride

[1337] Step 1: 5-bromo-N-phenylquinoline-3-carboxamide

[1338] 5 -Bromoquinolin-3 -carboxylic acid (300 mg, 1.19 mmol) was dissolved in dimethylformamide. To the resulting solution, aniline (133.0 mg, 1.42 mmol), HATU (O-(7- azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluranium hexafluorophosphate) (633.6 mg, 1.67 mmol), and diisopropylethylamine (769.16 mg, 5.95 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 1/1, v/v) to give a title compound (144.0 mg, 36.9 %). ¹ H-NMR (CDCI ₃ , 400MHz) δ 9.40 (dd, 1H), 8.97 (d, 1H), 8.16 (d, 1H), 7.99 (s, 1H), 7.94 (d, 1H), 7.73-7.68 (m, 3H)m 7.44 (t, 2H), 7.23 (t, 1H).

[1339] Step 2: tert-butyl 4-((3-(pheny1carbamoyl)quinolin-5-yl)amino)piperidine-1-carb oxylate

[1340] 5-Bromo-N-phenylquinoline-3-carboxamide (144.0 mg, 0.44 mmol) prepared in Step 1 was dissolved in anhydrous toluene. To the resulting solution, tert-butyl 4-aminopiperidine-1- carboxylate (88.2 mg, 0.44 mmol), cesium carbonate (103.1 mg, 0.53 mmol), tris(dibenzylideneacetone)dipalladium(0) (36.6 mg, 0.04 mmol), and (+/-)-2,2'- bis(diphenylphospino)-1,1'-binaphthalene (32.9 mg, 0.05 mmol) were added. The reaction mixture was refluxed and stirred at 100 °C overnight. After the completion of the reaction, the reaction mixture was cooled to room temperature and then added with water to extract an aqueous layer with ethyl acetate. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/n-hexane= 1/1, v/v) to give atitle compound (81.4 mg, 41.4 %). MS (ESI) m/z= 447.2 (M + H)+

[1341] Step 3: N-phenyl-5-(piperidin-4-ylamino)quinoline-3-carboxamide hydrochloride [1342] Tert-butyl 4-((3-(pheny1carbamoyl)quinolin-5-yl)amino)piperidine-1-carb oxylate (81.4 mg, 0.18 mmol) prepared in Step 2 was dissolved in dichloromethane (8.4 ml) and then 4.0 M of hydrochloric acid dissolved in 1,4-dioxane was added thereto in excess drops. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, the solid compound obtained therefrom was filtered, washed, and vacuum dried to prepare a title compound (5.4 mg,

7.2 %).

[1343] Reference Example 227. N-benzyl-N-methyl-5-(piperidin-4-ylamino)quinoline-3- carboxamide hydrochloride

[1344] Step 1: N-benzyl-5-bromo-N-methylquinoline-3-carboxamide

[1345] The title compound (172.2 mg, 40.7 %) was prepared in the same fashion as Step 1 of

Reference Example 226, except that N-methyl-1-phenylmethanamine (173.0 mg, 1.42 mmol) was used instead of aniline. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 9.04 (s, 1H), 8.64 (s, 1H), 8.11 (s, 1H),

7.89.7.86 (m, 1H), 7.64-7.62 (m, 1H), 7.43-7.35 (m, 4H), 7.24-7.23 (m, 1H), 4.73 (d, 2H), 3.08 (d, 3H).

[1346] Step 2: tert-butyl 4-((3 -(benzyl (methyl)carbamoyl)quinolin-5-yl)amino)piperidine-1- carboxylate

[1347] The title compound (120.8 mg, 52.4 %) was prepared in the same fashion as Step 2 of

Reference Example 226. MS (ESI) m/z= 475.3 (M + H)+

[1348] Step 3: N-benzyl-N-methyl-5-(piperidin-4-ylamino)quinoline-3-carboxa mide hydrochloride

[1349] The title compound (105.3 mg, 92.4 %) was prepared in the same fashion as Step 3 of

Reference Example 226 and used in the next step without any further purification.

[1350] Reference Example 228. N-methyl-5-(piperidin-4-ylamino)quinoline-8-carboxamide hydrochloride

[1351] Step 1: tert-butyl 4-((8-(methylcarbamoyl)quinolin-5-yl)amino)piperidine-1-carb oxylate

[1352] The title compound (116.9 mg, 42.0 %) was prepared in the same fashion as Step 2 of Example 140, except that tert-butyl 4-aminopiperidine-1-carboxylate (144.2 mg, 0.72 mmol) was used instead of tert-butyl (S)-3-aminopyrrolidine-1-carboxylate. MS (ESI) m/z= 385.2 (M + H)+

[1353] Step 2: N-methyl-5-(piperidin-4-ylamino)quinoline-8-carboxamide hydrochloride

[1354] The title compound (71.3 mg, 85.6 %) was prepared in the same fashion as Step 3 of

Reference Example 140 and used in the next step without any further purification. [1355] Reference Example 229. N,N-dimethyl-5-(piperidm-4-ylamino)quinoline-8-carboxamide hydrochloride

[1356] Step 1: tert-butyl 4-((8-(dimethylcarbamoyl)quinolin-5-yl)amino)piperidine-1-ca rboxylate

[1357] The title compound (84.9 mg, 32.9 %) was prepared in the same fashion as Step 2 of Example 141, except that tert-butyl 4-aminopiperidine-1-carboxylate (128.0 mg, 0.64 mmol) was used instead of tert-butyl (S)-3-aminopyrrolidine-1-carboxylate. MS (ESI) m/z= 399.2 (M + H)+

[1358] Step 2: N,N-dimethyl-5-(piperidin-4-ylamino)quinoline-8-carboxamide hydrochloride

[1359] The title compound (60.1 mg, 23.4 %) was prepared in the same fashion as Step 3 of

Reference Example 141 and used in the next step without any further purification

[1360] Reference Example 230. N-methyl-N-phenyl-5-(piperidin-4-ylamino)quinoline-8- carboxamide hydrochloride

[1361] Step 1: tert-butyl 4-((8-(methyl(phenyl)carbamoyl)quinolin-5-yl)amino)piperidin e-1- carboxylate

[1362] The title compound (196.6 mg, 39.9 %) was prepared in the same fashion as Step 2 of Example 143, except that tert-butyl 4-aminopiperidine-1-carboxylate (128.0 mg, 0.64 mmol) was used instead of tert-butyl (S)-3-aminopyrrolidine-1-carboxylate. MS (ESI) m/z= 461.3 (M + H)+

[1363] Step 2: N-methyl-N-phenyl-5-(piperidin-4-ylamino)quinoline-8-carboxa mide hydrochloride

[1364] The title compound (134.2 mg, 72.6 %) was prepared in the same fashion as Step 3 of

Reference Example 143 and used in the next step without any further purification

[1365] Reference Example 231. N-benzyl-N-methyl-5-(piperidin-4-ylamino)quinoline-8- carboxamide hydrochloride

[1366] Step 1: tert-butyl 4-((8-(benzyl(methyl)carbamoyl)quinolin-5-yl)amino)piperidin e-1- carboxylate

[1367] The title compound (218.1 mg, 32.4 %) was prepared in the same fashion as Step 2 of Example 144, except that tert-butyl 4-aminopiperidine-1-carboxylate (215.0 mg, 1.07 mmol) was used instead of tert-butyl (S)-3-aminopyrrolidine-1-carboxylate. MS (ESI) m/z= 475.3 (M + H)+

[1368] Step 2: N-methyl-N-phenyl-5-(piperidin-4-ylamino)quinoline-8-carboxa mide hydrochloride

[1369] The title compound (179.2 mg, 87.1 %) was prepared in the same fashion as Step 3 of

Reference Example 144 and used in the next step without any further purification [1370] Reference Example 232. N-phenyl-5-(piperidin-4-ylamino)quinoline-8-carboxamide hydrochloride

[1371] Step 1: tert-butyl 4-((8-(pheny1carbamoyl)quinolin-5-yl)amino)piperidine-1-carb oxylate

[1372] The title compound (196.9 mg, 41.6 %) was prepared in the same fashion as Step 2 of Example 142, except that tert-butyl 4-aminopiperidine-1-carboxylate (213.0 mg, 1.06 mmol) was used instead of tert-butyl (S)-3-aminopyrrolidine-1-carboxylate. MS (ESI) m/z= 447.2 (M + H)+

[1373] Step 2: N-phenyl-5-(piperidin-4-ylamino)quinoline-8-carboxamide hydrochloride

[1374] The title compound (100.8 mg, 54.4 %) was prepared in the same fashion as Step 3 of

Reference Example 142 and used in the next step without any further purification

[1375] Reference Example 233. 5-(piperidin-4-ylamino)-N-(pyridin-2-yl)quinoline-8- carboxamide hydrochloride

[1376] Step 1: tert-butyl 4-((8-(pyridin-2-y1carbamoyl)quinolin-5-yl)amino)piperidine- 1- carboxylate

[1377] The title compound (100.6 mg, 45.0 %) was prepared in the same fashion as Step 2 of Example 147, except that tert-butyl 4-aminopiperidine-1-carboxylate (100.0 mg, 0.50 mmol) was used instead of tert-butyl (S)-3-aminopyrrolidine-1-carboxylate. MS (ESI) m/z= 448.2 (M + H)+

[1378] Step 2: 5-(piperidin-4-ylamino)-N-(pyridin-2-yl)quinoline-8-carboxam ide hydrochloride

[1379] The title compound (63.5 mg, 67.6 %) was prepared in the same fashion as Step 3 of

Reference Example 147 and used in the next step without any further purification. ¹ H-NMR (MeOD ₄ , 400MHz) δ 9.40 (s, 1H), 9.18 (dd, 1H), 8.84 (d, 1H), 8.53-8.49 (m, 2H), 8.02 (d, 1H), 7.89 (dd, 1H), 7.67 (t, 1H), 7.17 (d, 1H), 4.22-4.17 (m, 1H), 3.58 (d, 2H), 3.30-3.26 (m, 2H), 2.39 (d, 2H), 2.12-2.02 (m, 2H).

[1380] Reference Example 234. 5-(piperidin-4-ylamino)-N-(pyridin-3-yl)quinoline-8- carboxamide hydrochloride

[1381] Step 1: tert-butyl 4-((8-(pyridin-3-y1carbamoyl)quinolin-5-yl)amino)piperidine- 1- carboxylate

[1382] The title compound (210.3 mg, 94.1 %) was prepared in the same fashion as Step 2 of Example 145, except that tert-butyl 4-aminopiperidine-1-carboxylate (100.0 mg, 0.50 mmol) was used instead of tert-butyl (S)-3-aminopyrrolidine-1-carboxylate. MS (ESI) m/z= 448.2 (M + H)+

[1383] Step 2: 5-(piperidin-4-ylamino)-N-(pyridin-3-yl)quinoline-8-carboxam ide hydrochloride

[1384] The title compound (195.3 mg, 98.9 %) was prepared in the same fashion as Step 3 of Reference Example 145 and used in the next step without any further purification. ¹ H-NMR (MeOD ₄ , 400MHz) δ 9.66 (d, 1H), 9.16 (dd, 1H), 8.88 (d, 1H), 8.80 (d, 1H), 8.73-8.71 (m, 1H), 8.63 (d, 1H), 8.14-8.11 (m, 1H), 7.94-7.88 (m, 1H), 7.17 (d, 1H), 4.22-4.17 (m, 1H), 3.58 (d, 2H), 3.27 (d, 2H),

2.40 (d, 2H), 2.03 (d, 2H).

[1385] Reference Example 235. 5-(piperidin-4-ylamino)-N-(pyridin-4-yl)quinoline-8- carboxamide hydrochloride

[1386] Step 1: tert-butyl 4-((8-(pyridin-4-y1carbamoyl)quinolin-5-yl)amino)piperidine- 1- carboxylate

[1387] The title compound (130.1 mg, 58.2 %) was prepared in the same fashion as Step 2 of Example 146, except that tert-butyl 4-aminopiperidine-1-carboxylate (100.0 mg, 0.50 mmol) was used instead of tert-butyl (S)-3-aminopyrrolidine-1-carboxylate. MS (ESI) m/z= 448.2 (M + H)+

[1388] Step 2: 5-(piperidin-4-ylamino)-N-(pyridin-4-yl)quinoline-8-carboxam ide hydrochloride

[1389] The title compound (68.6 mg, 56.0 %) was prepared in the same fashion as Step 3 of

Reference Example 146 and used in the next step without any further purification. ¹ H-NMR (MeOD ₄ , 400MHz) δ 9.34-9.24 (m, 1H), 9.15 (d, 1H), 8.79 (d, 1H), 8.67 (d, 2H), 8.47 (d, 2H), 7.84 (s, 1H),

7.12 (d, 1H), 4.18-4.12 (m, 1H), 3.57 (d, 2H), 3.26 (d, 2H), 2.40 (d, 2H), 2.07-1.96 (m, 2H).

[1390] Reference Example 236. 5-(piperidin-4-ylamino)-N-(pyrimidin-2-yl)quinoline-8- carboxamide hydrochloride

[1391] Step 1: 5-bromo-N-(pyrimidin-2-yl)quinoline-8-carboxamide

[1392] The title compound (140.1 mg, 21.4 %) was prepared in the same fashion as Step 1 of

Reference Example 140, except that pyrimidin-2 -amine (188.6 mg, 1.98 mmol) was used instead of methanamine. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 9.16 (dd, 1H), 8.87 (d, 1H), 8.79 (dd, 1H), 8.76 (d, 2H), 8.07 (d, 1H), 7.69 (dd, 1H), 7.08 (t, 1H).

[1393] Step 2: tert-butyl 4-((8-(pyrimidin-2-y1carbamoyl)quinolin-5-yl)amino)piperidin e-1- carboxylate

[1394] The title compound (144.4 mg, 75.8 %) was prepared in the same fashion as Step 1 of

Reference Example 233, except 5-bromo-N-(pyrimidin-2-yl)quinoline-8-carboxamide (140.3 mg,

0.43 mmol) prepared in Step 1 was used instead of 5-bromo-N-(pyridin-2-yl)quinoline-8- carboxamide. MS (ESI) m/z= 449.2 (M + H)+

[1395] Step 3: (S)-N-(pyridin-4-yl)-5-(pyrrolidin-3-ylamino)quinoline-8-car boxamide hydrochloride

[1396] The title compound (63.6 mg, 46.9 %) was prepared in the same fashion as Step 2 of Reference Example 233 and used in the next step without any further purification. ¹ H-NMR (MeOD ₄ , 400MHz) δ 9.32 (d, 1H), 9.13 (d, 1H), 9.01 (d, 2H), 8.78 (d, 1H), 7.85 (dd, 1H), 7.61 (t, 1H), 7.14 (d, 1H), 4.20-4.14 (m, 1H), 3.58 (d, 2H), 3.27 (d, 2H), 2.40 (d, 2H), 2.10-2.00 (m, 2H).

[1397] Reference Example 237. N-(isoxazol-3-yl)-5-(piperidin-4-ylamino)quinoline-8- carboxamide hydrochloride

[1398] Step 1: 5-bromo-N-(isoxazol-3-yl)quinoline-8-carboxamide

[1399] The title compound (158.8 mg, 41.9 %) was prepared in the same fashion as Step 1 of

Reference Example 140, except that isoxazol-3 -amine (118.0 mg, 1.42 mmol) was used instead of methanamine. ¹ H-NMR (CDCI ₃ , 400MHz) δ 9.09 (dd, 1H), 8.78 (dd, 2H), 8.38 (dd, 1H), 8.05 (dd ,1H), 7.69 (dd, 1H), 7.29 (d, 1H).

[1400] Step 2: tert-butyl 4-((8-(isoxazol-3-y1carbamoyl)quinolin-5-yl)amino)piperidine -1- carboxylate

[1401] The title compound (84.9 mg, 38.8 %) was prepared in the same fashion as Step 1 of

Reference Example 233, except 5-bromo-N-(isoxazol-3-yl)quinoline-8-carboxamide (158.8 mg, 0.5 mmol) prepared in Step 1 was used instead of 5-bromo-N-(pyridin-2-yl)quinoline-8-carboxamide. MS (ESI) m/z= 438.2 (M + H)+

[1402] Step 3: N-(isoxazol-3-yl)-5-(piperidin-4-ylamino)quinoline-8-carboxa mide hydrochloride

[1403] The title compound (21.6 mg, 27.3 %) was prepared in the same fashion as Step 2 of

Reference Example 233 and used in the next step without any further purification.

[1404] Reference Example 238. N-(5-(piperidin-4-ylamino)quinolin-8-yl)acetamide hydrochloride

[1405] Step 1: tert-butyl 4-((8-acetamidoquinolin-5-yl)amino)piperidine-1-carboxylate

[1406] The title compound (264.2 mg, 27.5 %) was prepared in the same fashion as Step 1 of

Reference Example 28, except that N-(5-bromoquinolin-8-yl)acetamide (661.8 mg, 2.49 mmol) was used instead of 4-bromoquinoline. MS (ESI) m/z= 385.2 (M + H)+

[1407] Step 2: N-(5-(piperidin-4-ylamino)quinolin-8-yl)acetamide hydrochloride

[1408] The title compound (204.2 mg, 83.1 %) was prepared in the same fashion as Step 2 of

Reference Example 28 and used in the next step without any further purification. ¹ H-NMR (MeOD ₄ , 400MHz) δ 9.54 (dd, 1H), 9.00 (dd, 1H), 7.95 (dd, 1H), 7.79 (d, 1H), 7.08 (d, 1H), 4.02-3.05 (m, 1H), 3.55 (m, 2H), 3.25 (td, 2H), 2.39 (d, 2H), 2.30 (s, 3H), 2.00-1.89 (m, 2H).

[1409] Reference Example 239. 6-methyl-4-(piperidin-4-yloxy)quinoline hydrochloride [1410] Step 1: tert-butyl 4-((6-methylquinolin-4-yl)oxy)piperidine-1-carboxylate

[1411] The title compound (425.3 mg, 100.0 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 4-hydroxy-6-methyl quinoline (197.7 mg, 1.24 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 343.2 (M + H)+

[1412] Step 2: 6-methyl-4-(piperidin-4-yloxy)quinoline hydrochloride

[1413] The title compound (346.3 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1414] Reference Example 240. 4-(piperidin-4-yloxy)-6-(trifluoromethyl)quinoline hydrochloride

[1415] Step 1: tert-butyl 4-((6-(trifluoromethyl)quinolin-4-yl)oxy)piperidine-1-carbox ylate

[1416] The title compound (492.3 mg, 100.0 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 6-(trifluoromethyl)quinolin-4-ol (264.8 mg, 1.24 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 397.1 (M + H)+

[1417] Step 2: 4-(piperidin-4-yloxy)-6-(trifluoromethyl)quinoline hydrochloride

[1418] The title compound (413.3 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1419] Reference Example 241. 4-(piperidin-4-yloxy)-2-(trifluoromethyl)quinoline hydrochloride

[1420] Step 1: tert-butyl 4-((2-(trifluoromethyl)quinolin-4-yl)oxy)piperidine-1-carbox ylate

[1421] The title compound (347.0 mg, 70.5 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 4-hydroxy-2-trifluoromethylquinoline (264.8 mg, 1.24 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 397.1 (M + H)+

[1422] Step 2: 4-(piperidin-4-yloxy)-2-(trifluoromethyl)quinoline hydrochloride

[1423] The title compound (291.3 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1424] Reference Example 242. 6-fluoro-4-(piperidin-4-yloxy)-2-(trifluoromethyl)quinoline hydrochloride

[1425] Step 1: tert-butyl 4-((6-fluoro-2-(trifluoromethyl)quinolin-4-yl)oxy)piperidine -1- carboxylate

[1426] The title compound (302.0 mg, 58.7 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 6-fluoro-2-(trifluoromethyl)quinolin-4-ol (287.1 mg, 1.24 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 415.2 (M + H)+ [1427] Step 2: 6-fluoro-4-(piperidin-4-yloxy)-2-(trifluoromethyl)quinoline hydrochloride

[1428] The title compound (255.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1429] Reference Example 243. 6-methoxy-4-(piperidin-4-yloxy)-2-(trifluoromethyl)quinoline hydrochloride

[1430] Step 1: tert-butyl 4-((6-methoxy-2-(trifluoromethyl)quinolin-4-yl)oxy)piperidin e-1- carboxylate

[1431] The title compound (357.1 mg, 67.4 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 6-methoxy-2-(trifluoromethyl)quinolin-4-ol (302.1 mg, 1.24 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 427.2 (M + H)+

[1432] Step 2: 6-methoxy-4-(piperidin-4-yloxy)-2-(trifluoromethyl)quinoline hydrochloride

[1433] The title compound (303.8 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1434] Reference Example 244. 8-methoxy-4-(piperidin-4-yloxy)-2-(trifluoromethyl)quinoline hydrochloride

[1435] Step 1: tert-butyl 4-((8-methoxy-2-(trifluoromethyl)quinolin-4-yl)oxy)piperidin e-1- carboxylate

[1436] The title compound (487.7 mg, 92.1 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 8-methoxy-2-(trifluoromethyl)quinolin-4-ol (302.1 mg, 1.24 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 427.2 (M + H)+

[1437] Step 2: 8-methoxy-4-(piperidin-4-yloxy)-2-(trifluoromethyl)quinoline hydrochloride

[1438] The title compound (414.9 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1439] Reference Example 245. 3-fluoro-5-(piperidin-4-yloxy)quinoline hydrochloride

[1440] Step 1: tert-butyl 4-((3-fluoroquinolin-5-yl)oxy)piperidine-1-carboxylate

[1441] The title compound (430.2 mg, 100.0 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 3-fluoroquinolin-5-ol (202.7 mg, 1.24 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 347.2 (M + H)+

[1442] Step 2: 3-fluoro-5-(piperidin-4-yloxy)quinoline hydrochloride

[1443] The title compound (442.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of Reference Example 31 and used in the next step without any further purification.

[1444] Reference Example 246. 5-(piperidin-4-yloxy)quinoline-2-carbonitrile hydrochloride

[1445] Step 1: tert-butyl 4-((2-cyanoquinolin-5-yl)oxy)piperidine-1-carboxylate

[1446] The title compound (438.9 mg, 100.0 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 5-hydroxyquinoline-2-carbonitrile (211.4 mg, 1.24 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 354.2 (M + H)+

[1447] Step 2: 5-(piperidin-4-yloxy)quinoline-2-carbonitrile hydrochloride

[1448] The title compound (381.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1449] Reference Example 247. 8-chloro-5-(piperidin-4-yloxy)quinoline hydrochloride

[1450] Step 1: tert-butyl 4-((8-chloroquinolin-5-yl)oxy)piperidine-1-carboxylate

[1451] The title compound (450.7 mg, 100.0 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 8-chloroquinolin-5-ol (223.1 mg, 1.24 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 363.2 (M + H)+

[1452] Step 2: 8-chloro-5-(piperidin-4-yloxy)quinoline hydrochloride

[1453] The title compound (660.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1454] Reference Example 248. l-(piperidin-4-yloxy)isoquinoline hydrochloride

[1455] Step 1: tert-butyl 4-(isoquinolin-1-yloxy)piperidine-1-carboxylate

[1456] The title compound (155.8 mg, 38.2 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 1-hydroxyisoquinoline (180.3 mg, 1.24 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 329.2 (M + H)+

[1457] Step 2: 1-(piperidin-4-yloxy)isoquinoline hydrochloride

[1458] The title compound (125.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1459] Reference Example 249. 4-(piperidin-4-yloxy)isoquinoline hydrochloride

[1460] Step 1: tert-butyl 4-(isoquinolin-4-yloxy)piperidine-1-carboxylate

[1461] The title compound (407.9 mg, 100.0 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 4-hydroxyisoquinoline (180.3 mg, 1.24 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 329.1 (M + H)+ [1462] Step 2: 4-(piperidin-4-yloxy)isoquinoline hydrochloride

[1463] The title compound (328.8 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1464] Reference Example 250. 5-(piperidin-4-yloxy)isoquinoline hydrochloride

[1465] Step 1: tert-butyl 4-(isoquinolin-5-yloxy)piperidine-1-carboxylate

[1466] The title compound (194.1 mg, 47.6 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 5-hydroxyisoquinoline (180.3 mg, 1.24 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 329.1 (M + H)+

[1467] Step 2: 5-(piperidin-4-yloxy)isoquinoline hydrochloride

[1468] The title compound (156.5 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1469] Reference Example 251. 6-(piperidin-4-yloxy)isoquinoline hydrochloride

[1470] Step 1: tert-butyl 4-(isoquinolin-6-yloxy)piperidine-1-carboxylate

[1471] The title compound (407.9 mg, 100.0 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 6-hydroxyisoquinoline (180.3 mg, 1.24 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 329.2 (M + H)+

[1472] Step 2: 6-(piperidin-4-yloxy)isoquinoline hydrochloride

[1473] The title compound (328.9 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1474] Reference Example 252. 7-(piperidin-4-yloxy)isoquinoline hydrochloride

[1475] Step 1: tert-butyl 4-(isoquinolin-7-yloxy)piperidine-1-carboxylate

[1476] The title compound (407.9 mg, 100.0 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 7-hydroxyisoquinoline (180.3 mg, 1.24 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 329.2 (M + H)+

[1477] Step 2: 7-(piperidin-4-yloxy)isoquinoline hydrochloride

[1478] The title compound (328.9 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1479] Reference Example 253. 6-methoxy-2-methyl-4-(piperidin-4-yloxy)quinoline hydrochloride

[1480] Step 1: tert-butyl 4-((6-methoxy-2-methylquinolin-4-yl)oxy)piperidine-1-carboxy late [1481] The title compound (462.6 mg, 100.0 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 6-methoxy-2-methylquinolin-4-ol (235.0 mg, 1.24 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 373.2 (M + H)+

[1482] Step 2: 6-methoxy-2-methyl-4-(piperidin-4-yloxy)quinoline hydrochloride

[1483] The title compound (383.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1484] Reference Example 254. 7-chloro-4-(piperidin-4-yloxy)quinoline hydrochloride

[1485] Step 1: tert-butyl 4-((7-chloroquinolin-4-yl)oxy)piperidine-1-carboxylate

[1486] The title compound (450.7 mg, 99.9 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 7-chloroquinolin-4-ol (223.1 mg, 1.24 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 363.2 (M + H)+

[1487] Step 2: 7-chloro-4-(piperidin-4-yloxy)quinoline hydrochloride

[1488] The title compound (371.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1489] Reference Example 255. 7-methyl-4-(piperidin-4-yloxy)quinoline hydrochloride

[1490] Step 1: tert-butyl 4-((7-methylquinolin-4-yl)oxy)piperidine-1-carboxylate

[1491] The title compound (500.0 mg, 98.0 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 7-methylquinolin-4-ol (237.3 mg, 1.49 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 343.3 (M + H)+

[1492] Step 2: 7-methyl-4-(piperidin-4-yloxy)quinoline hydrochloride

[1493] The title compound (407.0 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1494] Reference Example 256. 7-fluoro-4-(piperidin-4-yloxy)quinoline hydrochloride

[1495] Step 1: tert-butyl 4-((7-fluoroquinolin-4-yl)oxy)piperidine-1-carboxylate

[1496] The title compound (516.0 mg, 99.9 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 7-fluoroquinolin-4-ol (243.2 mg, 1.49 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 347.2 (M + H)+

[1497] Step 2: 7-fluoro-4-(piperidin-4-yloxy)quinoline hydrochloride

[1498] The title compound (421.3 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification. [1499] Reference Example 257. 4-(piperidin-4-yloxy)quinoline-8-carbonitrile hydrochloride

[1500] Step 1: tert-butyl 4-((8-cyanoquinolin-4-yl)oxy)piperidine-1-carboxylate

[1501] The title compound (505.4 mg, 95.9 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 4-hydroxyquinoline-8-carbonitrile (253.7 mg, 1.49 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 354.2 (M + H)+

[1502] Step 2: 4-(piperidin-4-yloxy)quinoline-8-carbonitrile hydrochloride

[1503] The title compound (414.4 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1504] Reference Example 258. 8-fluoro-4-(piperidin-4-yloxy)quinoline hydrochloride

[1505] Step 1: tert-butyl 4-((8-fluoroquinolin-4-yl)oxy)piperidine-1-carboxylate

[1506] The title compound (516.0 mg, 99.9 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 8-fluoro-4-hydroxyquinoline (243.2 mg, 1.49 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 347.1 (M + H)+

[1507] Step 2: 8-fluoro-4-(piperidin-4-yloxy)quinoline hydrochloride

[1508] The title compound (421.3 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1509] Reference Example 259. 2-methyl-4-(piperidin-4-yloxy)quinoline hydrochloride

[1510] Step 1: tert-butyl 4-((2-methylquinolin-4-yl)oxy)piperidine-1-carboxylate

[1511] The title compound (510.0 mg, 99.9 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 4-hydroxy-2-methyl-quinoline (237.3 mg, 1.49 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 343.2 (M + H)+

[1512] Step 2: 2-methyl-4-(piperidin-4-yloxy)quinoline hydrochloride

[1513] The title compound (415.4 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1514] Reference Example 260. 6-fluoro-2-methyl-4-(piperidin-4-yloxy)quinoline hydrochloride

[1515] Step 1: tert-butyl 4-((6-fluoro-2-methylquinolin-4-yl)oxy)piperidine-1-carboxyl ate

[1516] The title compound (537.0 mg, 99.9 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 6-fluoro-2-methyl-quinolin-4-ol (264.1 mg, 1.49 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 361.2 (M + H)+

[1517] Step 2: 6-fluoro-2-methyl-4-(piperidin-4-yloxy)quinoline hydrochloride [1518] The title compound (442.2 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1519] Reference Example 261. 8-methoxy-4-(piperidin-4-yloxy)quinoline hydrochloride

[1520] Step 1: tert-butyl 4-((8-methoxyquinolin-4-yl)oxy)piperidine-1-carboxylate

[1521] The title compound (118.7 mg, 22.2 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 4-hydroxy-8-methoxyquinoline (261.1 mg, 1.49 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 359.2 (M + H)+

[1522] Step 2: 8-methoxy-4-(piperidin-4-yloxy)quinoline hydrochloride

[1523] The title compound (97.6 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1524] Reference Example 262. 8-methyl-4-(piperidin-4-yloxy)quinoline hydrochloride

[1525] Step 1: tert-butyl 4-((8-methylquinolin-4-yl)oxy)piperidine-1-carboxylate

[1526] The title compound (510.0 mg, 99.9 %) was prepared in the same fashion as Step 1 of

Reference Example 31, except that 8-methylquinolin-4-ol (237.3 mg, 1.49 mmol) was used instead of 3-quinolinol. MS (ESI) m/z= 343.2 (M + H)+

[1527] Step 2: 8-methyl-4-(piperidin-4-yloxy)quinoline hydrochloride

[1528] The title compound (415.4 mg, 100.0 %) was prepared in the same fashion as Step 2 of

Reference Example 31 and used in the next step without any further purification.

[1529] Example 1. (R)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)-N-(quinoli n-3- yl)pyrrolidine-3-carboxamide

[1530] (S)-1-(2-Chloroacetyl)pyrrolidine-2-carbonitrile (30 mg, 0.17 mmol) prepared in

Reference Example 1 was dissolved in anhydrous dichloromethane (1.2 ml). To the resulting solution, (R)-N-(quinolin-3-yl)pyrrolidine-3-carboxamide hydrochloride (54.6 mg, 0.19 mmol) prepared in

Reference Example 24 and potassium carbonate (96.0 mg, 0.69 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/methanol= 10/1, v/v) to give the title compound (31.6 mg). ¹ H-NMR (CDCI ₃ , 400MHz) δ 10.45 (s, 1H), 9.12 (s, 1H), 8.75 (s, 1H), 7.97 (d, 1H), 7.73 (d, 1H), 7.55-7.46 (m, 2H), 4.78 (s, 1H), 3.74-3.18 (m, 7H), 2.82 (s, 1H), 2.59-2.18 (m, 8H) [1531] Example 2. (R)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)-N-(quinoli n-4- yl)pyrrolidine-3-carboxamide

[1532] The title compound (33.3 mg) was prepared in the same fashion as Example 1, except that

(R)-N-(quinolin-4-yl)pyrrolidine-3-carboxamide hydrochloride (54.6 mg, 0.19 mmol) prepared in

Reference Example 23 was used instead of (R)-N-(quinolin-3-yl)pyrrolidine-3-carboxamide hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 10.62 (s, 1H), 8.78 (s, 1H), 8.31-8.26 (m, 2H), 8.05 (d, 1H), 7.71-7.62 (m, 2H), 4.78 (d, 1H), 3.71-3.19 (m, 8H), 2.82 (s, 1H), 2.62-2.05 (m, 8H)

[1533] Example 3. (S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)-N-(quinoli n-3- yl)pyrrolidine-3-carboxamide

[1534] The title compound (6.3 mg) was prepared in the same fashion as Example 1, except that

(S)-N-(quinolin-3-yl)pyrrolidine-3-carboxamide hydrochloride (54.6 mg, 0.19 mmol) prepared in

Reference Example 22 was used instead of (R)-N-(quinolin-3-yl)pyrrolidine-3-carboxamide hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 10.36 (d, 1H), 9.11 (d, 1H), 8.84 (d, 1H), 8.02 (t, 1H), 7.81 (d, 1H), 7.60-7.47 (m, 2H), 4.83 (d, 1H), 3.69-3.04 (m, 7H), 2.56-2.05 (m, 10H)

[1535] Example 4. (S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)-N-(quinoli n-4- yl)pyrrolidine-3-carboxamide

[1536] The title compound (14.3 mg) was prepared in the same fashion as Example 1, except that (S)-N-(quinolin-4-yl)pyrrolidine-3-carboxamide hydrochloride (54.6 mg, 0.19 mmol) prepared in

Reference Example 21 was used instead of (R)-N-(quinolin-3-yl)pyrrolidine-3-carboxamide hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 10.42 (s, 1H), 8.77 (d, 1H), 8.35-8.03 (m, 3H), 7.71- 7.58 (m, 2H), 4.79 (d, 1H), 3.70-3.35 (m, 6H), 3.22 (s, 1H), 2.48-2.05 (m, 9H)

[1537] Example 5. (S)-1-(2-((S)-3-(quinolin-4-ylamino)pyrrolidin-1-yl)acetyl)p yrrolidine-2- carbonitrile

[1538] (S)-1-(2-Chloroacetyl)pyrrolidine-2-carbonitrile (20 mg, 0.12 mmol) prepared in

Reference Example 1 was dissolved in anhydrous dichloromethane (4 ml). To the resulting solution, (S)-N-(pynOlidin-3-yl)quinolin-4-amine hydrochloride (39.8 mg, 0.14 mmol) prepared in Reference Example 5 and potassium carbonate (48.0 mg, 0.35 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol= 10/1, v/v) to give the title compound (11.4 mg). ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.65-8.72 (m, 1H), 8.00 (d, 1H), 7.87-7.93 (m, 1H), 7.77-7.82 (m, 1H), 7.67-7.75 (m, 1H), 7.58-7.62 (m, 1H), 4.75-4.82 (m, 1H), 4.25-4.31 (m, 1H), 3.63-3.70 (m, 1H), 3.44-3.52 (m, 2H), 3.12- 3.22 (m, 1H), 3.08 (d, 1H), 2.80 (dd, 0.3H), 2.64-2.71 (m, 0.5H), 2.26-2.36 (m, 2H), 2.13-2.25 (m,

2H)

[1539] Example 6. (S)-4,4-difluoro-1-(2-((S)-3-(quinolin-3-ylamino)pyrrolidin- 1- yl)acetyl)pyrrolidine-2-carbonitrile

[1540] (2S)-1-(2-Chloroacetyl)-4,4-difluoro-pyrrolidine-2-carbonitr ile (25.0 mg 0.12 mmol) prepared in Reference Example 3 was dissolved in anhydrous dichloromethane (4 ml). To the resulting solution, (S)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride (34.3 mg 0.12 mmol) prepared in Reference Example 6 and potassium carbonate (48.0 mg, 0.35 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol= 10/1, v/v) to give the title compound (6.1 mg). ¹ H- NMR (CDCI ₃ , 400MHz) δ 8.42 (s, 1H), 7.85-7.96 (m, 1H), 7.50-7.64 (m, 1H), 7.36-7.46 (m, 2H),

6.96 (s, 1H), 5.17-5.28 (m, 0.4H), 4.89-5.01 (m, 0.6H), 4.32-4.56 (m, 1H), 4.05-4.19 (m, 2H), 3.37- 3.55 (m, 1H), 3.30-3.36 (m, 1H), 2.91-3.11 (m, 2H), 2.68-2.86 (m, 3H), 2.33-2.53 (m, 2H), 1.78-1.99 (m, 2H)

[1541] Example 7. (S)-4,4-difluoro-1-(2-((S)-3-(quinolin-4-ylamino)pyrrolidin- 1- yl)acetyl)pyrrolidine-2-carbonitrile

[1542] The title compound (5.3 mg) was prepared in the same fashion as Example 6, except that (S)-N-(pynOlidin-3-yl)quinolin-4-amine hydrochloride (34.3 mg 0.12 mmol) prepared in Reference Example 5 was used instead of (S)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.68 (s, 1H), 7.90 (d, 1H), 7.85 (d, 1H), 7.76-7.83 (m, 1H), 7.62-7.72 (m, 1H), 7.54-7.62 (m, 1H), 5.13-5.21 (m, 0.4H), 4.91-5.02 (m, 0.6H), 4.65-4.80 (m, 1H), 4.26 (m, 1H), 3.98- 4.15 (m, 2H), 3.78-3.91 (m, 0.5H), 3.47-3.59 (m, 0.5H), 3.34-3.43 (m, 1.5H), 2.87-3.14 (m, 3H), 2.59- 2.81 (m, 3H), 2.35-2.59 (m, 1.5H)

[1543] Example 8. (S)-4,4-difluoro-1-(2-((S)-3-(isoquinolin-4-ylamino)pyrrolid in-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1544] The title compound (16.3 mg) was prepared in the same fashion as Example 6, except that

(S)-N-(pyrrolidin-3-yl)isoquinolin-4-amine hydrochloride (34.3 mg, 0.12 mmol) prepared in

Reference Example 7 was used instead of (S)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.51 (d, 1H), 7.96 (d, 1H), 7.83-7.91 (m, 1H), 7.59-7.65 (m, 1H), 7.42

(t, 1H), 5.92 (d, 0.6H), 5.69 (d, 0.4H), 5.11 (dd, 0.4H), 4.91 (t, 0.6H), 4.16-4.28 (m, 1H), 3.72-4.12 (m, 2H), 3.59 (d, 0.5H), 3.37 (s, 1H), 3.31 (d, 0.5H), 3.07-3.18 (m, 0.5H), 2.99-3.07 (m, 1H), 2.89- 2.95 (m, 1H), 2.55-2.76 (m, 5H), 2.44-2.55 (m, 0.5H), 2.30-2.41 (m, 1H), 1.92 (m, 1H)

[1545] Example 9. (S)-1-(2-((S)-3-(benzo[b]thiophen-4-ylamino)pyrrolidin-1-yl) acetyl)-4,4- difluoropyrrolidine-2-carbonitrile

[1546] The title compound (5.62 mg) was prepared in the same fashion as Example 6, except that (S)-N-(benzo[b]thiophen-4-yl)pyrrolidin-3-amine hydrochloride (26.2 mg, 0.12 mmol) prepared in

Reference Example 8 was used instead of (S)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.23-7.36 (m, 3H), 7.16-7.23 (m, 1H), 6.35 (s, 1H), 5.15 (d, 0.4H), 4.88 (t, 0.6H), 4.18 (s, 1H), 3.94-4.05 (m, 1.6H), 3.81-3.91 (m, 0.5H), 3.44-3.53 (m, 0.5H), 3.31-3.40 (m, 0.5H), 3.25-3.31 (m, 1H), 3.03-3.11 (m, 0.4H), 2.86-2.99 (m, 1.6H), 2.76-2.82 (m, 0.5H), 2.29-2.72 (m, 5H), 1.79-1.98 (m, 1H)

[1547] Example 10. (S)-1-(2-((S)-3-(benzo[b]thiophen-7-ylamino)pyrrolidin-1-yl) acetyl)-4,4- difluoropyrrolidine-2-carbonitrile

[1548] The title compound (10.9 mg) was prepared in the same fashion as Example 6, except that (S)-N-(benzo[b]thiophen-7-yl)pyrrolidin-3-amine hydrochloride (26.2 mg, 0.12 mmol) prepared in

Reference Example 9 was used instead of (S)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.34-7.41 (m, 1H), 7.20-7.34 (m, 4H), 6.47-6.57 (m, 1H), 5.37 (d, 0.5H), 4.89 (t, 0.7H), 4.17-4.28 (m, 1H), 3.75-4.08 (m, 3H), 3.35-3.55 (m, 1H), 3.21-3.35 (m, 1H), 2.78-3.13 (m, 2H), 2.55-2.73 (m, 4H), 2.31-2.53 (m, 2H), 1.78-1.97 (m, 1H)

[1549] Example 11. (S)-1-(2-((S)-3-(benzofuran-7-ylamino)pyrrolidin-1-yl)acetyl )-4,4- difluoropyrrolidine-2-carbonitrile

[1550] The title compound (6.91 mg) was prepared in the same fashion as Example 6, except that (S)-N-(benzofuran-7-yl)pyrrolidin-3-amine hydrochloride (24.27 mg, 0.12 mmol) prepared in

Reference Example 10 was used instead of (S)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.55 (s, 1H), 7.08 (t, 1H), 6.97 (d, 1H), 6.72 (s, 1H), 6.45-6.50 (m, 1H), 5.44 (d, 0.3H), 4.90 (dd, 0.6H), 4.13-4.29 (m, 1H), 3.75-4.08 (m, 2H), 3.32-3.59 (m, 1H), 3.23-3.32 (m, 1H), 2.85-3.08 (m, 2H), 2.55-2.78 (m, 4H), 2.32-2.53 (m, 1H), 1.72-1.93 (m, 1H)

[1551] Example 12. (S)-4,4-difluoro-1-(2-((S)-3-(furo[3,2-c]pyridin-7-ylamino)p yrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1552] The title compound (4.59 mg) was prepared in the same fashion as Example 6, except that (S)-N-(pynOlidin-3-yl)furo[3,2-c]pyridin-7-amine hydrochloride (24.4 mg, 0.12 mmol) prepared in

Reference Example 11 was used instead of (S)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.39 (s, 1H), 7.87 (s, 1H), 7.61-7.72 (m, 1H), 6.82-6.98 (m, 1H), 5.31- 5.41 (m, 0.4H), 4.92-5.03 (m, 0.7H), 4.21-4.45 (m, 2H), 3.49-3.62 (m, 1H), 3.33-3.40 (m, 1H), 2.94- 3.12 (m, 2H), 2.58-2.87 (m, 6H), 2.37-2.54 (m, 2H), 1.77-2.00 (m, 1H)

[1553] Example 13. (S)-1-(2-((S)-3-([1,1'-biphenyl]-2-ylamino)pyrrolidin-1-yl)a cetyl)-4,4- difluoropyrrolidine-2-carbonitrile

[1554] The title compound (14.9 mg) was prepared in the same fashion as Example 6, except that (S)-N-([1,1'-biphenyl]-2-yl)pyrrolidin-3-amine hydrochloride (28.6 mg, 0.12 mmol) prepared in

Reference Example 12 was used instead of (S)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.32-7.48 (m, 5H), 7.19-7.26 (m, 1H), 7.09 (d, 1H), 6.78 (br t, 1H),

6.64 (d, 1H), 5.36 (br d, 0.3H), 4.87-4.92 (m, 0.6H), 3.75-4.22 (m, 4H), 3.57 (br d, 0.5H), 3.20-3.30 (m, 1.6H), 2.79-2.93 (m, 1H), 2.62-2.76 (m, 2H), 2.43-2.61 (m, 2H), 2.24-2.35 (m, 1H), 1.56-1.70 (m, 1H)

[1555] Example 14. (S)-1-(2-((S)-3-([1,1'-biphenyl]-4-ylamino)pyrrolidin-1-yl)a cetyl)-4,4- difluoropyrrolidine-2-carbonitrile

[1556] The title compound (13.3 mg) was prepared in the same fashion as Example 6, except that (S)-N-([1,1'-biphenyl]-4-yl)pyrrolidin-3-amine hydrochloride (28.6 mg, 0.12 mmol) prepared in

Reference Example 13 was used instead of (S)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.53 (d, 2H), 7.41-7.51 (m, 2H), 7.39 (t, 2H), 7.22-7.30 (m, 1H), 6.65 (d, 2H), 5.31-5.48 (s, 0.4H), 4.95-5.12 (m, 0.6H), 3.96-4.17 (m, 3H), 3.33-3.52 (m, 1H), 2.84-3.08 (m, 2H), 2.56-2.81 (m, 4H), 2.27-2.53 (m, 2H), 1.78-1.85 (m, 1H)

[1557] Example 15. (S)-4,4-difluoro-1-(2-((S)-3-(naphthalen-2-ylamino)pyrrolidi n-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1558] The title compound (13.8 mg) was prepared in the same fashion as Example 6, except that (S)-N-(naphthalen-2-yl)pyrrolidin-3-amine (25.5 mg, 0.12 mmol) prepared in Reference Example 14 was used instead of (S)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.58-7.68 (m, 3H), 7.36 (t, 1H), 7.16-7.26 (m, 1H), 6.85 (d, 1H), 6.73-6.81 (m, 1H), 5.26 (d, 0.3H), 4.89 (t, 0.6H), 4.08-4.19 (m, 1H), 3.82-3.92 (m, 0.5H), 3.37-3.57 (m, 1H), 3.23-3.33 (m, 1H), 2.87-3.04 (m, 1.5H), 2.55-2.83 (m, 4H), 2.30-2.47 (m, 1H), 1.81-1.93 (m, 1H)

[1559] Example 16. (S)-1-(2-((S)-3-([1,1'-biphenyl]-3-ylamino)pyrrolidin-1-yl)a cetyl)-4,4- difluoropyrrolidine-2-carbonitrile

[1560] The title compound (8.3 mg) was prepared in the same fashion as Example 6, except that (S)-N-([1,1'-biphenyl]-3-yl)pyrrolidin-3-amine hydrochloride (28.6 mg, 0.12 mmol) prepared in

Reference Example 15 was used instead of (S)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.55 (d, 2H), 7.41 (t, 2H), 7.30-7.35 (m, 1H), 7.20-7.25 (m, 1H), 6.93 (d, 1H), 6.77 (s, 1H), 6.54-6.62 (m, 1H), 5.28 (m, 0.3H), 4.90 (m, 0.6H), 3.95-4.14 (m, 3H), 3.77-3.87 (m, 0.4H), 3.42-3.52 (m, 0.6H), 3.23-3.29 (m, 1H), 2.84-3.01 (m, 1H), 2.54-2.78 (m, 4H), 2.29-2.44 (m, 1H), 1.68-1.84 (m, 1H)

[1561] Example 17. (S)-1-(2-((R)-3-(quinolin-3-ylamino)pyrrolidin-1-yl)acetyl)p yrrolidine-2- carbonitrile

[1562] (S)-1-(2-Chloroacetyl)pyrrolidine-2-carbonitrile (25.0 mg 0.12 mmol) prepared in

Reference Example 1 was dissolved in anhydrous dichloromethane (4 ml). To the resulting solution,

(R)-N-(pynOlidin-3-yl)quinolin-3-amine hydrochloride (34.3 mg 0.12 mmol) prepared in Reference Example 17 and potassium carbonate (48.0 mg, 0.35 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol= 10/1, v/v) to give the title compound (6.1 mg). ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.39-8.54 (m, 1H), 7.93 (d, 1H), 7.60-7.72 (m, 1H), 7.37-7.44 (m, 2.0H), 6.96 (s, 1H), 4.83-4.91 (m, 0.4H), 4.76-4.81 (m, 1H), 4.50 (m, 0.6H), 3.67-3.81 (m, 1H), 3.46-3.56 (m, 1H), 3.28- 3.43 (m, 2H), 3.02-3.12 (m, 2H), 2.80-2.90 (m, 1H), 2.52-2.73 (m, 1H), 2.10-2.48 (m, 5H), 1.81 (m, 2H)

[1563] Example 18. (S)-1-(2-((R)-3-([1,1'-biphenyl]-3-ylamino)pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1564] The title compound (6.92 mg) was prepared in the same fashion as Example 17, except that (R)-N-([1,1'-biphenyl]-3-yl)pyrrolidin-3-amine hydrochloride (28.6 mg, 0.12 mmol) prepared in

Reference Example 18 was used instead of (R)-N-(pyrrolidin-3-yl)quinolin-3 -amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.56 (d, 2H), 7.41 (t, 2H), 7.33 (d, 1H), 7.20-7.32 (m, 1H), 6.92 (d,

1H), 6.79 (s, 1H), 6.58 (d, 1H), 4.89-4.96 (m, 0.3H), 4.74 (m, 0.7H), 4.11-4.24 (m, 1H), 3.65-3.72 (m, 1H), 3.29-3.56 (m, 4H), 2.88-3.02 (m, 2H), 2.79-2.85 (m, 1H), 2.63-2.71 (m, 1H), 2.04-2.45 (m, 5H), 1.78-1.85 (m, 1H)

[1565] Example 19. (S)-1-(2-((S)-3-(isoquinolin-4-ylamino)pyrrolidin-1-yl)acety l)pyrrolidine-2- carbonitrile

[1566] The title compound (4.78 mg) was prepared in the same fashion as Example 5, except that

(S)-N-(pyrrolidin-3-yl)isoquinolin-4-amine hydrochloride (25.6 mg, 0.12 mmol) prepared in

Reference Example 7 was used instead of (S)-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.41-8.54 (m, 1H), 7.93-8.22 (m, 2H), 7.65-7.75 (m, 1H), 7.39-7.53 (m,

1H), 7.36-7.41 (m, 0.5H), 6.34-6.44 (m, 1H), 6.22-6.32 (m, 0.4H), 4.72-4.86 (m, 1H), 4.24-4.34 (m, 1H), 3.43-3.72 (m, 4H), 3.24-3.36 (m, 1H), 2.98-3.17 (m, 2H), 2.83-2.94 (m, 1H), 1.93-2.54 (m, 7H)

[1567] Example 20. (S)-1-(2-((S)-3-(benzo[b]thiophen-4-ylamino)pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1568] The title compound (8.46 mg) was prepared in the same fashion as Example 5, except that (S)-N-(benzo[b]thiophen-4-yl)pyrrolidin-3-amine hydrochloride (26.2 mg, 0.12 mmol) prepared in

Reference Example 8 was used instead of (S)-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.33-7.42 (m, 1H), 7.28-7.32 (m, 1H), 7.20-7.25 (m, 1H), 7.17-7.20 (m, 1H), 6.44 (dd, 1H), 4.63-4.88 (m, 1H), 4.19-4.24 (m, 1H), 3.26-3.69 (m, 4H), 2.98-3.18 (m, 1H), 2.82- 2.95 (m, 1H), 2.62-2.76 (m, 1H), 1.83-2.52 (m, 7H)

[1569] Example 21. (S)-1-(2-((S)-3-(benzo[b]thiophen-7-ylamino)pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1570] The title compound (6.55 mg) was prepared in the same fashion as Example 5, except that (S)-N-(benzo[b]thiophen-7-yl)pyrrolidin-3-amine hydrochloride (26.2 mg, 0.12 mmol) prepared in

Reference Example 9 was used instead of (S)-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.24-7.37 (m, 4H), 6.50-6.72 (m, 1H), 4.99-5.02 (m, 0.4H), 4.74-4.82 (m, 0.6H), 4.24-4.32 (m, 1H), 3.97-4.13 (m, 1H), 3.57-3.68 (m, 1H), 3.24-3.52 (m, 3H), 3.03-3.10 (m, 1H), 2.95-3.01 (m, 0.6H), 2.75-2.84 (m, 1H), 2.62-2.71 (m, 0.6H), 2.34-2.46 (m, 1H), 1.75-2.28 (m, 5H)

[1571] Example 22. (S)-1-(2-((S)-3-(benzofuran-7-ylamino)pyrrolidin-1-yl)acetyl )pyrrolidine-2- carbonitrile

[1572] The title compound (22.5 mg) was prepared in the same fashion as Example 5, except that (S)-N-(benzofuran-7-yl)pyrrolidin-3-amine hydrochloride (24.27 mg, 0.12 mmol) prepared in

Reference Example 10 was used instead of (S)-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.54-7.65 (m, 1H), 7.08 (t, 1H), 6.96 (d, 1H), 6.72 (s, 1H), 6.47-6.52 (m, 1H), 5.06-5.12 (m, 0.3H), 4.74-4.85 (m, 0.6H), 4.18-4.30 (m, 1H), 3.58-3.68 (m, 1H), 3.44-3.56 (m, 1H), 3.24-3.42 (m, 2H), 2.89-3.14 (m, 2H), 2.65-2.87 (m, 2H), 2.33-2.51 (m, 1H), 1.94-2.29 (m, 4H), 1.78-1.91 (m, 1H)

[1573] Example 23. (S)-1-(2-((S)-3-(furo[3,2-c]pyridin-7-ylamino)pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1574] The title compound (14.0 mg) was prepared in the same fashion as Example 5, except that (S)-N-(pynOlidin-3-yl)furo[3,2-c]pyridin-7-amine hydrochloride (24.4 mg, 0.12 mmol) prepared in

Reference Example 11 was used instead of (S)-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.37 (s, 1H), 7.87 (s, 1H), 7.60-7.71 (m, 1H), 6.80-6.95 (m, 1H), 4.96- 5.02 (m, 0.3H), 4.77-4.88 (m, 0.7H), 4.37-4.51 (m, 1H), 4.30-4.35 (m, 1H), 3.59-3.72 (m, 1H), 3.40- 3.55 (m, 1H), 2.97-3.11 (m, 2H), 2.80-2.89 (m, 1.4H), 2.60-2.68 (m, 0.6H), 2.04-2.55 (m, 6H), 1.81- 1.95 (m, 1H)

[1575] Example 24. (S)-1-(2-((R)-3-(isoquinolin-4-ylamino)pyrrolidin-1-yl)acety l)pyrrolidine-2- carbonitrile

[1576] The title compound (12.4 mg) was prepared in the same fashion as Example 17, except that (R)-N-(pyrrolidin-3-yl)isoquinolin-4-amine hydrochloride prepared in Reference Example 19 was used instead of (R)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) d 8.52-8.62 (m, 1H), 7.94-8.01 (m, 2H), 7.59-7.65 (m, 1H), 7.39-7.47 (m, 1H), 6.38-6.54 (m, 1H), 4.76-4.84 (m, 1H), 4.14-4.35 (m, 1H), 3.60-3.68 (m, 1H), 3.33-3.58 (m, 3H), 3.13-3.23 (m, 1H), 2.90- 3.10 (m, 2H), 2.58-2.78 (m, 1H), 2.08-2.50 (m, 5H), 1.87-2.00 (m, 1H)

[1577] Example 25. (S)-1-(2-((R)-3-(benzo[b]thiophen-7-ylamino)pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1578] The title compound (4.5 mg) was prepared in the same fashion as Example 17, except that (R)-N-(benzo[b]thiophen-7-yl)pyrrolidin-3-amine hydrochloride (26.2 mg, 0.12 mmol) prepared in

Reference Example 20 was used instead of (R)-N-(pyrrolidin-3-yl)quinolin-3 -amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.24-7.38 (m, 4H), 6.54-6.72 (m, 1H), 5.02-5.12 (m, 0.4H), 4.75-4.82 (m, 0.7H), 4.25-4.32 (m, 1H), 3.61-3.88 (m, 1H), 3.47-3.58 (m, 1H), 3.28-3.46 (m, 2H), 2.87-3.10 (m, 2H), 2.79-2.87 (m, 1H), 2.59-2.73 (m, 1H), 2.32-2.50 (m, 1H), 2.04-2.30 (m, 4H), 1.79-1.93 (m, 1H)

[1579] Example 26. (1S,3S,5S)-2-(2-((S)-3-(furo[3,2-c]pyridin-7-ylamino)pyrroli din-1- yl)acetyl)-2-azabicyclo[3.1.0]hexane-3-carbonitrile

[1580] (1S,3S,5S)-2-(2-Chloroacetyl)-2-azabicyclo[3.1.0]hexane-3-ca rbonitrile (22.2 mg, 0.12 mmol) prepared in Reference Example 4 was dissolved in anhydrous acetonitrile (4 ml). To the resulting solution, (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7-amine hydrochloride (33.1 mg, 0.12 mmol) prepared in Reference Example 11 and potassium carbonate (48.0 mg, 0.35 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol= 10/1, v/v) to give the title compound (1.8 mg). ¹ H- NMR (CDCI ₃ , 400MHz) δ 8.84 (m, 1H), 8.37 (d, 0.6H), 8.28 (d, 0.4H), 7.54-7.72 (m, 1H), 7.46-7.52 (m, 1H), 7.33-7.42 (m, 1H), 6.56-6.72 (m, 1H), 5.42-5.51 (m, 0.6H), 5.09-5.24 (m, 0.4H), 4.75-4.92 (m, 1H), 4.20-4.31 (m, 1H), 3.62-3.78 (m, 1H), 3.24-3.57 (m, 3H), 2.92-3.20 (m, 3H), 2.59-2.76 (m, 1H), 1.86-2.49 (m, 6H) [1581] Example 27. (S)-1-(2-((S)-3-(quinolin-5-ylamino)pyrrolidin-1-yl)acetyl)p yrrolidine-2- carbonitrile

[1582] The title compound (18.1 mg) was prepared in the same fashion as Example 5, except that (S)-N-(pynOlidin-3-yl)quinolin-5-amine hydrochloride (34.3 mg, 0.12 mmol) prepared in Reference Example 16 was used instead of (S)-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.84 (m, 1H), 8.37 (d, 0.6H), 8.28 (d, 0.4H), 7.54-7.72 (m, 1H), 7.46-7.52 (m, 1H), 7.33-7.42 (m, 1H), 6.56-6.72 (m, 1H), 5.42-5.51 (m, 0.6H), 5.09-5.24 (m, 0.4H), 4.75-4.92 (m, 1H), 4.20-4.31 (m, 1H), 3.62-3.78 (m, 1H), 3.24-3.57 (m, 3H), 2.92-3.20 (m, 3H), 2.59-2.76 (m, 1H), 1.86-2.49 (m, 6H)

[1583] Example 28. (2S,4S)-4-fluoro-1-(2-((S)-3-(furo[3,2-c]pyridin-7-ylamino)p yrrolidin-1- yl)acetyl)pyrrolidin-2-carbonitrile;

[1584] (2S,4S)-1-(2-Chloroacetyl)-4-fluoro-pyrrolidin-2-carbonitril e (20.0 mg, 0.10 mmol) prepared in Reference Example 2 was dissolved in anhydrous dichloromethane (4 ml). To the resulting solution, (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7-amine hydrochloride (31.8 mg 0.12 mmol) prepared in Reference Example 11 and potassium carbonate (29.0 mg, 0.21 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol = 10/1, v/v) to give the title compound (27.7 mg). ¹ H NMR (CD ₃ OD, 400 MHz) δ 8.36 (s, 1H), 7.89 (d, 1H), 7.13 (s, 1H), 6.98 (d, 1H), 5.51-5.38 (m, 1H), 5.00 (dd, 1H), 4.60 (s, 1H), 4.03 (dd, 1H), 3.85-3.73 (m, 1H), 3.58-3.39 (m, 2H), 3.07-3.04 (m, 1H) 2.92-2.84 (m, 2H), 2.69-2.31 (m, 4H), 1.88-1.86 (m, 1H)

[1585] Example 29. N-((S)-1-(2-((2S,4S)-2-cyano-4-fluoropyrrolidin-1-yl)-2- oxoethyl)pyrrolidin-3 -yl)-6-fluorobenzofuran-3 -carboxamide ;

[1586] (2S,4S)-1-(2-Chloroacetyl)-4-fluoro-pyrrolidin-2-carbonitril e (20.0 mg, 0.10 mmol) prepared in Reference Example 2 was dissolved in anhydrous acetonitrile (1 ml). To the resulting solution, (S)-6-fluoro-N-(pyrrolidin-3-yl)benzofuran-3-carboxamide hydrochloride (32.8 mg 0.12 mmol) prepared in Reference Example 45 and potassium carbonate (29.0 mg, 0.21 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol = 10/1, v/v) to give the title compound (4.3 mg). ¹ H NMR (CD ₃ OD, 400 MHz) 8 δ.36 (s, 1H), 8.03 (s, 1H), 7.35 (d, 1H), 7.15 (t, 1H), 5.51-5.38 (m, 1H), 5.00 (dd, 1H), 4.60 (s, 1H), 4.03 (dd, 1H), 3.85-3.73 (m, 1H), 3.64-3.36 (m, 2H), 3.07-3.04 (m, 1H) 2.92-2.86 (m, 2H), 2.69-2.32 (m, 4H), 1.88-1.86 (m, 1H)

[1587] Example 30. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3- yl)quinoline-4-carboxamide

[1588] (S)-1-(2-((S)-3-Aminopyrrolidin-1-yl)acetyl)pyrrolidine-2-ca rbonitrile trifluoroacetate (20 mg, 0.09 mmol) prepared in Reference Example 25 was dissolved in dimethylformamide (2 ml). To the resulting solution, quinoline-4-carboxylic acid (31.2 mg, 0.18 mmol), HATU (0(7- azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluranium hexafluorophosphate) (24.9 mg, 0.18 mmol), and diisopropylethylamine(47uL, 0.27 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with ethyl acetate. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol= 20/1, v/v) to give the title compound (7 mg). ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.92-9.01 (m, 1H), 8.33 (d, 1H), 8.14 (d, 1H), 7.76 (t, 1H), 7.49-7.69 (m, 2H), 4.77-7.89 (m, 1H), 4.68-4.75 (m, 1H), 3.55-3.67 (m, 1H),

3.43 (q, 2H), 3.18 (m, 1H), 2.99 (m, 2H), 2.58-2.83 (m, 1H), 2.07-2.51 (m, 5H), 1.78-2.02 (m, 3H)

[1589] Example 31. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3- yl)quinoline-3 -carboxamide

[1590] The title compound (7.23 mg) was prepared in the same fashion as Example 30, except that quinoline-3 -carboxylic acid (31.2 mg, 0.18 mmol) was used instead of quinoline-4-carboxylic acid. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 9.41-9.50 (m, 1H), 8.73-8.85 (m, 1H), 8.12-8.21 (m, 1H), 7.97- 8.11 (m, 1H), 7.78-7.95 (m, 1H), 7.60-7.72 (m, 1H), 4.74-4.88 (m, 2H), 3.36-3.78 (m, 4H), 3.26 -3.32 (m, 2H), 3.02-3.12 (m, 1H), 2.73-2.82 (m, 1H), 2.13-2.45 (m, 5H), 2.05-2.12 (m, 2H)

[1591] Example 32. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3- yl)quinoline-5 -carboxamide

[1592] The title compound (11.7 mg) was prepared in the same fashion as Example 30, except that quinoline-5 -carboxylic acid (31.2 mg, 0.18 mmol) was used instead of quinoline-4-carboxylic acid. ¹ H-NMR (CD ₃ OD, 400MHz): Shift = 9.23 (d, 0.3H), 8.83-8.94 (m, 1H), 8.76 (d, 0.7H), 8.12 (d, 1H), 7.81-7.92 (m, 2H), 7.61-7.80 (m, 1H), 5.07-5.15 (m, 0.2H), 4.78-4.87 (m, 0.5H), 4.67-4.77 (m, 0.7H), 3.66-3.75 (m, 1H), 3.46-3.65 (m, 2H), 3.33-3.42 (m, 1H), 2.72-3.23 (m, 4H), 2.44-2.52 (m, 1H), 1.89-2.26 (m, 4H), 1.24-1.40 (m, 2H)

[1593] Example 33. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3- yl)quinoline-2 -carboxamide

[1594] The title compound (14.4 mg) was prepared in the same fashion as Example 30, except that quinoline-2 -carboxylic acid (31.2 mg, 0.18 mmol) was used instead of quinoline-4-carboxylic acid. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.67-8.81 (m, 1H), 8.29-8.42 (m, 2H), 8.12-8.24 (m, 1H), 7.87- 8.10 (m, 1H), 7.77-7.86 (m, 1H), 7.62-7.72 (m, 1H), 5.11-5.19 (m, 0.3H), 4.78-7.85 (m, 1.7H), 3.60- 3.77 (m, 1H), 3.54-3.59 (m, 1H), 3.42-3.51 (m, 2H), 3.27-3.42 (m, 1H), 3.01-3.11 (m, 1H), 2.82-2.92 (m, 1H), 2.62-2.72 (m, 1H), 2.54-2.60 (m, 1H), 2.17-2.42 (m, 4H), 1.94-2.02 (m, 1H), 1.44-1.59 (m, 1H)

[1595] Example 34. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3- yl)quinoline-8-carboxamide

[1596] The title compound (4.82 mg) was prepared in the same fashion as Example 30, except that quinoline-8-carboxylic acid (31.2 mg, 0.18 mmol) was used instead of quinoline-4-carboxylic acid. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.92-9.02 (m, 1H), 8.75-8.88 (m, 1H), 8.21-8.34 (m, 1H), 7.92- 8.03 (m, 1H), 7.61-7.73 (m, 1H), 7.45-7.59 (m, 1H), 4.61-4.91 (m, 1.7H), 3.33-3.82 (m, 4H), 2.73- 3.24 (m, 3H), 2.41-2.63 (m, 1H), 1.87-2.34 (m, 6H)

[1597] Example 35. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3- yl)isoquinoline- 1 -carboxamide

[1598] The title compound (4.9 mg) was prepared in the same fashion as Example 30, except that isoquinoline-1 -carboxylic acid (31.2 mg, 0.18 mmol) was used instead of quinoline-4-carboxylic acid. ¹ H-NMR (CDCI ₃ , 400MHz) δ 9.55-9.68 (m, 1H), 8.45-8.58 (m, 2H), 7.83-7.98 (m, 1H), 7.75-7.81 (m, 1H), 7.61-7.74 (m, 2H), 5.18-5.26 (m, 0.3H), 4.77-4.87 (m, 0.7H), 4.64-4.74 (m, 1H), 3.57-3.71 (m, 2H), 3.40-3.55 (m, 1H), 3.10-3.28 (m, 1H), 2.85-2.96 (m, 2H), 2.39-2.72 (m, 2H), 2.17-2.35 (m, 3H), 1.84-2.10 (m, 2H)

[1599] Example 36. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3- yl)benzo[b]thiophene-2 -carboxamide

[1600] The title compound (8.0 mg) was prepared in the same fashion as Example 30, except that benzo[b]thiophene-2-carboxylic acid (32.6 mg, 0.18 mmol) was used instead of quinoline-4- carboxylic acid. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.20-8.29 (m, 0.5H), 7.97-8.12 (m, 0.6H), 7.80-7.89 (m, 2H), 7.39-7.52 (m, 2H), 4.77-4.89 (m, 1H), 4.67-4.72 (m, 1H), 3.39-3.73 (m, 3H), 3.06-3.25 (m, 2H), 2.93-3.00 (m, 1H), 2.71-2.82 (m, 1H), 2.13-2.47 (m, 5H), 1.95 (m, 1H)

[1601] Example 37. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3- yl)benzofuran-3 -carboxamide

[1602] The title compound (6.4 mg) was prepared in the same fashion as Example 30, except that benzofuran-3 -carboxylic acid (29.2 mg, 0.18 mmol) was used instead of quinoline-4-carboxylic acid. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.26-8.36 (m, 0.7H), 8.14-8.21 (m, 1.4H), 7.81-7.92 (m, 0.6H), 7.52- 7.68 (m, 1H), 7.35-7.51 (m, 2H), 7.05-7.13 (m, 0.3H), 4.75-4.82 (m, 2H), 3.64-3.82 (m, 2H), 3.39- 3.52 (m, 3H), 3.00-3.26 (m, 2H), 2.94-3.00 (m, 1H), 2.69-2.85 (m, 1H), 2.11-2.46 (m, 6H), 1.87-2.02 (m, 1H)

[1603] Example 38. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3-yl)-2-

(naphthalen- 1 -yl)acetamide

[1604] The title compound (6.6 mg) was prepared in the same fashion as Example 30, except that 2-(naphthalen-1-yl)acetic acid (31.2 mg, 0.18 mmol) was used instead of quinoline-4-carboxylic acid. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.95-8.03 (m, 1H), 7.79-7.90 (m, 2H), 7.41-7.57 (m, 4H), 6.06-6.33 (m, 1H), 4.62 (m, 0.6H), 4.45 (m, 1H), 3.92-4.07 (m, 2H), 3.36-3.52 (m, 1H), 3.18-3.30 (m, 2H), 2.64- 3.08 (m, 2H), 2.32-2.55 (m, 2H), 2.07-2.30 (m, 4H), 1.48-1.58 (m, 1H)

[1605] Example 39. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3-yl)-[1,1'- biphenyl]-2-carboxamide

[1606] The title compound (7.6 mg) was prepared in the same fashion as Example 30, except that [1,1'-biphenyl] -2 -carboxylic acid (35.7 mg, 0.18 mmol) was used instead of quinoline-4-carboxylic acid. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.66-7.76 (m, 1H), 7.34-7.50 (m, 8H), 5.87-6.07 (m, 1H), 4.40- 4.78 (m, 2H), 3.56-3.72 (m, 2H), 3.06-3.30 (m, 2H), 2.65-2.81 (m, 1H), 2.58-2.64 (m, 1H), 2.19-2.39 (m, 3H), 2.03-2.18 (m, 4H), 1.25-1.35 (m, 2H)

[1607] Example 40. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3- yl)benzofuran-2 -carboxamide

[1608] The title compound (8.4 mg) was prepared in the same fashion as Example 30, except that benzofuran-2 -carboxylic acid (29.2 mg, 0.18 mmol) was used instead of quinoline-4-carboxylic acid. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.66-7.75 (m, 1H), 7.49-7.59 (m, 1H), 7.37-7.49 (m, 2H), 7.27-7.33 (m, 1H), 4.91 (m, 0.3H), 4.79 (m, 0.7H), 4.71-4.78 (m, 1H), 3.62-3.71 (m, 1H), 3.41-3.60 (m, 2H), 3.08- 3.28 (m, 1H), 2.88-2.98 (m, 2H), 2.52-2.83 (m, 1H), 2.03-2.42 (m, 5H), 1.79-1.98 (m, 1H)

[1609] Example 41. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3- yl)benzo [b]thiophene -3 -carboxamide

[1610] The title compound (10.4 mg) was prepared in the same fashion as Example 30, except that benzo[b]thiophene -3 -carboxylic acid (32.1 mg, 0.18 mmol) was used instead of quinoline-4- carboxylic acid. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.49-8.58 (m, 1H), 8.08 (s, 0.6H), 7.96 (s, 0.4H), 7.85-7.95 (m, 1H), 7.68-7.82 (m, 0.6H), 7.34-7.48 (m, 2H), 7.10 (m, 0.4H), 4.72-4.82 (m, 2H), 3.39- 3.75 (m, 4H), 3.10-3.26 (m, 1H), 2.88-3.07 (m, 2H), 2.56-2.77 (m, 1H), 2.09-2.50 (m, 5H), 1.86-2.02 (m, 1H)

[1611] Example 42. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3-yl)-2- methylbenzofuran-3 -carboxamide

[1612] The title compound (14.4 mg) was prepared in the same fashion as Example 30, except that 2-methylbenzofuran-3 -carboxylic acid (31.7 mg, 0.18 mmol) was used instead of quinoline-4- carboxylic acid. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 7.68-7.82 (m, 1H), 7.43-7.52 (m, 1H), 7.27-7.33 (m, 1H), 6.97-7.12 (m, 0.7H), 6.58-6.71 (m, 0.3H), 4.69-4.91 (m, 2H), 3.60-3.73 (m, 1H), 3.32-3.59 (m, 3H), 3.08-3.24 (m, 1H), 2.92-3.05 (m, 2H), 2.78-2.90 (m, 1H), 2.71-2.75 (m, 3H), 2.65-2.71 (m, 1H), 2.08-2.49 (m, 5H), 1.86-2.00 (m, 1H)

[1613] Example 43. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3-yl)-5- fluorobenzofuran-3 -carboxamide

[1614] The title compound (6.5 mg) was prepared in the same fashion as Example 30, except that

5 -fluorobenzofuran-3 -carboxylic acid (32.4 mg, 0.18 mmol) was used instead of quinoline-4- carboxylic acid. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.33 (s, 0.6H), 8.16-8.22 (m, 0.3H), 8.12-8.14 (m, 0.7H), 7.74-7.92 (m, 1H), 7.43-7.52 (m, 1H), 6.95-7.17 (m, 1H), 4.64-4.86 (m, 2H), 3.49-3.72 (m, 3H), 3.43 (m, 1H), 3.21-3.35 (m, 1H), 3.16 (m, 1H), 2.91-3.10 (m, 1H), 2.69-2.86 (m, 1H), 2.13-2.40 (m, 5H)

[1615] Example 44. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3-yl)-6- fluorobenzofuran-3 -carboxamide

[1616] The title compound (9.8 mg) was prepared in the same fashion as Example 30, except that

6-fluorobenzofuran-3 -carboxylic acid (32.4 mg, 0.18 mmol) was used instead of quinoline-4- carboxylic acid. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.29 (s, 1H), 8.01-8.18 (m, 2H), 7.21-7.40 (m, 1H), 7.05-7.17 (m, 1H), 4.73-4.85 (m, 2H), 3.48-3.71 (m, 3H), 3.36-3.47 (m, 1H), 3.06-3.29 (m, 2H), 2.94- 3.05 (m, 1H), 2.66-2.80 (m, 1H), 2.11-2.39 (m, 5H), 1.84-2.03 (m, 1H)

[1617] Example 45. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3-yl)-7- methylbenzofuran-3 -carboxamide

[1618] The title compound (8.5 mg) was prepared in the same fashion as Example 30, except that

7-methylbenzofuran-3 -carboxylic acid (31.7 mg, 0.18 mmol) was used instead of quinobne-4- carboxylic acid. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.29 (s, 0.7H), 8.17 (s, 0.4H), 7.80-8.00 (m, 2H), 7.20-7.25 (m, 1H), 7.14-7.18 (m, 1H), 4.69-4.84 (m, 2H), 3.53-3.69 (m, 3H), 3.27-3.49 (m, 2H), 3.11- 3.25 (m, 1H), 2.77-2.97 (m, 1H), 2.52-2.62 (s, 3H), 2.10-2.42 (m, 5H), 1.99-2.04 (m, 1H)

[1619] Example 46. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3-yl)-5- methoxybenzofuran-3 -carboxamide [1620] The title compound (5.9 mg) was prepared in the same fashion as Example 30, except that

5 -methoxybenzofuran-3 -carboxylic acid (34.6 mg, 0.18 mmol) was used instead of quinoline-4- carboxylic acid. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.27 (s, 0.7H), 8.12 (s, 0.4H), 8.00 (d, 0.7H), 7.60- 7.69 (m, 1H), 7.38 (d, 1H), 7.09-7.24 (m, 0.4H), 6.93 (d, J=9.1, 1H), 4.68-4.81 (m, 2H), 3.88 (s, 3H), 3.50-3.70 (m, 3H), 3.25-3.49 (m, 2H), 2.98-3.23 (m, 2H), 2.68-2.79 (m, 1H), 2.11-2.41 (m, 5H), 1.97- 2.03 (m, 1H)

[1621] Example 47. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3-yl)-7- methoxybenzofuran-3 -carboxamide

[1622] The title compound (1.1 mg) was prepared in the same fashion as Example 30, except that 7 -methoxybenzofuran-3 -carboxylic acid (34.6 mg, 0.18 mmol) was used instead of quinoline-4- carboxylic acid. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.28 (s, 0.6H), 8.16 (s, 0.5H), 7.79-8.15 (m, 0.5H), 7.61-7.74 (m, 1H), 7.28-7.33 (m, 0.6H), 6.86 (d, 1.), 4.76-4.87 (m, 2H), 4.01-4.21 (s, 3H), 3.52-3.72 (m, 3H), 3.44-3.51 (m, 1H), 3.33-3.42 (m, 1H), 3.16-3.32 (m, 1H), 3.08-3.15 (m, 1H), 2.73-2.86 (m, 1H), 2.11-2.45 (m, 5H), 1.90-2.04 (m, 1H)

[1623] Example 48. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3-yl)-4- hydroxybenzofuran-3 -carboxamide

[1624] The title compound (1.8 mg) was prepared in the same fashion as Example 30, except that 4-hydroxybenzofuran-3-carboxylic acid (32.1 mg, 0.18 mmol) was used instead of quinoline-4- carboxylic acid. ¹ H-NMR (CDCI ₃ , 400MHz) δ 9.15-9.21 (m, 0.8H), 8.36 (s, 0.7H), 8.13 (s, 0.3H), 7.88 (m, 0.2H), 7.19-7.25 (m, 1H), 6.98-7.12 (m, 1H), 6.76 (d, 1H), 4.77-4.88 (m, 1H), 4.67-4.76 (m, 1H), 3.54-3.75 (m, 3H), 3.35-3.51 (m, 1H), 3.15-3.28 (m, 2H), 3.02 (m, 1H), 2.67-2.90 (m, 1H), 2.14- 2.42 (m, 5H), 2.05 (s, 1H)

[1625] Example 49. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3-yl)-6- hydroxybenzofuran-3 -carboxamide

[1626] The title compound (7.6 mg) was prepared in the same fashion as Example 30, except that

6-hydroxybenzofuran-3-carboxylic acid (32.1 mg, 0.18 mmol) was used instead of quinoline-4- carboxylic acid. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.05-8.12 (m, 1H), 7.80-7.98 (m, 1H), 7.64-7.78 (m, 1H), 6.99-7.12 (m, 1H), 6.82-6.98 (m, 1H), 4.76-4.82 (m, 2H), 3.48-3.84 (m, 3H), 3.41-3.47 (m, 1H), 3.31-3.40 (m, 1H), 2.91-3.23 (m, 2H), 2.62-2.81 (m, 1H), 2.11-2.40 (m, 5H), 1.86-2.01 (m, 1H)

[1627] Example 50. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3-yl)-7- fluorobenzofuran-3 -carboxamide

[1628] The title compound (9.6 mg) was prepared in the same fashion as Example 30, except that

7-fluorobenzofuran-3 -carboxylic acid (32.4 mg, 0.18 mmol) was used instead of quinoline-4- carboxylic acid. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.32 (s, 0.6H), 8.18 (s, 0.3H), 8.04-8.12 (m, 0.6H), 7.88-7.98 (m, 1.0H), 7.18-7.34 (m, 1.4H), 7.03-7.14 (m, 1H), 4.67-4.85 (m, 2H), 3.35-3.78 (m, 4H), 3.05-3.27 (m, 2H), 2.98-3.03 (m, 1H), 2.66-2.79 (m, 1H), 2.12-2.48 (m, 5H), 1.87-2.03 (m, 1H)

[1629] Example 51. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3-yl)-5,7- difluorobenzofuran-3 -carboxamide

[1630] The title compound (12 mg) was prepared in the same fashion as Example 30, except that 5, 7-difluorobenzofuran-3 -carboxylic acid (35.6 mg, 0.18 mmol) was used instead of quinoline-4- carboxylic acid. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.38 (s, 0.7H), 8.31 (d, 0.6H), 8.21 (s, 0.3H), 7.67- 7.77 (m, 1H), 7.36 (d, 0.3H), 6.88 (t, 1H), 4.77-4.89 (m, 1H), 4.65-4.73 (m, 1H), 3.36-3.74 (m, 4H), 3.08-3.28 (m, 2H), 3.00-3.07 (m, 1H), 2.69-2.82 (m, 1H), 2.13-2.41 (m, 5H), 1.86-2.05 (m, 1H)

[1631] Example 52. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3-yl)-5- methylbenzofuran-3 -carboxamide

[1632] The title compound (6.2 mg) was prepared in the same fashion as Example 30, except that

5 -methylbenzofuran-3 -carboxylic acid (31.7 mg, 0.18 mmol) was used instead of quinoline-4- carboxylic acid. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.22 (s, 0.6H), 8.10 (s, 0.4H), 7.91 (s, 1H), 7.69 (m, 0.7H), 7.38 (d, 1H), 7.15 (d, 1H), 7.03 (m, 0.3H), 4.67-4.82 (m, 2H), 3.37-3.77 (m, 4H), 2.89-3.32 (m, 3H), 2.63-2.85 (m, 1H), 2.45-2.50 (m, 3H), 2.12-2.39 (m, 5H), 1.97-2.11 (m, 1H)

[1633] Example 53. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3-yl)-6- methylbenzofuran-3 -carboxamide

[1634] The title compound (3.3 mg) was prepared in the same fashion as Example 30, except that

6-methylbenzofuran-3 -carboxylic acid (31.7 mg, 0.18 mmol) was used instead of quinoline-4- carboxylic acid. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.20 (s, 0.7H), 8.08 (s, 0.3H), 7.88-8.04 (m, 1H),

7.75 (m, 0.7H), 7.28-7.36 (m, 1H), 7.10-7.21 (m, 1H), 7.04 (m, 0.3H), 4.67-4.81 (m, 2H), 3.52-3.74 (m, 3H), 3.38-3.52 (m, 1H), 3.22-3.36 (m, 1H), 2.96-3.21 (m, 2H), 2.72-2.83 (m, 1H), 2.48 (s, 3H), 2.12-2.39 (m, 5H), 2.00-2.11 (m, 1H)

[1635] Example 54. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3-yl)-6- methoxybenzofuran-3 -carboxamide

[1636] The title compound (1.8 mg) was prepared in the same fashion as Example 30, except that 6-methoxybenzofuran-3-carboxylic acid (34.6 mg, 0.18 mmol) was used instead of quinoline-4- carboxylic acid. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.16 (s, 0.7H), 7.93-8.08 (m, 1.4H), 7.75 (m, 0.7H), 6.94-7.08 (m, 2.4H), 4.67-4.83 (m, 2H), 3.86 (s, 3H), 3.50-3.71 (m, 3H), 3.44-3.49 (m, 1H), 2.94-3.32 (m, 3H), 2.73-2.91 (m, 1H), 2.12-2.40 (m, 5H), 1.99-2.11 (m, 1H)

[1637] Example 55. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3- yl)benzofuran-4-carboxamide

[1638] The title compound (7.0 mg) was prepared in the same fashion as Example 30, except that benzofuran-4-carboxylic acid (29.2 mg, 0.18 mmol) was used instead of quinoline-4-carboxylic acid of Example 28. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 7.50-7.74 (m, 3H), 7.29-7.44 (m, 2H), 4.75-4.86 (m, 2H), 3.35-3.71 (m, 4H), 2.93-3.34 (m, 3H), 2.62-2.80 (m, 1H), 2.32-2.52 (m, 3H), 2.17-2.31 (m, 3H), 1.95-2.13 (m, 2H)

[1639] Example 56. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3- yl)benzofuran-5 -carboxamide

[1640] The title compound (10.6 mg) was prepared in the same fashion as Example 30, except that benzofuran-5 -carboxylic acid (29.2 mg, 0.18 mmol) was used instead of quinoline-4-carboxylic acid. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.11-8.24 (m, 1H), 7.76-7.99 (m, 1.7H), 7.66-7.75 (m, 1H), 7.53-7.62 (m, 1H), 7.06-7.24 (m, 0.4H), 6.80-6.90 (m, 1H), 4.75 (m, 2H), 3.39-3.73 (m, 4H), 2.84- 3.31 (m, 4H), 2.70-2.82 (m, 1H), 2.09-2.47 (m, 5H), 1.94-2.08 (m, 1H)

[1641] Example 57. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3- yl)benzofuran-6-carboxamide

[1642] The title compound (15.0 mg) was prepared in the same fashion as Example 30, except that benzofuran-6-carboxylic acid (29.2 mg, 0.18 mmol) was used instead of quinoline-4-carboxylic acid. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.00-8.15 (m, 1H), 7.57-7.87 (m, 3.7H), 7.15-7.24 (m, 0.4H), 6.80-6.92 (m, 1H), 4.76-4.86 (m, 2H), 3.38-3.75 (m, 4H), 3.10-3.27 (m, 1H), 2.80-3.05 (m, 2H), 2.56- 2.78 (m, 1H), 2.07-2.47 (m, 6H), 1.93-2.05 (m, 1H)

[1643] Example 58. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3- yl)benzofuran-7 -carboxamide

[1644] The title compound (11.2 mg) was prepared in the same fashion as Example 30, except that benzofuran-7-carboxylic acid (29.2 mg, 0.18 mmol) was used instead of quinoline-4-carboxylic acid. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.08 (m, 1H), 7.68-7.95 (m, 3H), 7.30-7.41 (m, 1H), 6.87 (s, 1H), 5.06 (m, 0.4H), 4.78-4.89 (m, 1.6H), 3.59-3.78 (m, 1H), 3.30-3.55 (m, 3H), 3.14-3.28 (m, 1H), 2.77-3.03 (m, 2H), 2.38-2.69 (m, 2H), 2.01-2.35 (m, 5H), 1.93-2.00 (m, 1H)

[1645] Example 59. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3- yl)benzofuran-2-sulfonamide

[1646] (S)-1-(2-((S)-3-Aminopyrrolidin-1-yl)acetyl)pyrrolidine-2-ca rbonitrile trifluoroacetate (30 mg, 0.13 mmol) prepared in Reference Example 25 was dissolved in anhydrous dichloromethane (2 ml). To the resulting solution, triethylamine (60 uL, 0.40 mmol) and benzofuran 2-sulfonyl chloride

(32.2 mg, 0.15 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (ethyl acetate/methanol= 20/1, v/v) to give the title compound (7.3 mg). ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.68-7.79 (m, 1H), 7.56-7.62 (m, 1H), 7.46-7.55 (m, 1H), 7.31-7.40 (m, 2H), 4.68-4.80 (m, 1H), 4.09-4.12 (m, 1H), 3.55-3.77 (m, 1H), 3.21-3.53 (m, 3H), 2.74- 3.16 (m, 3H), 2.42-2.69 (m, 1H), 2.02-2.40 (m, 7H), 1.76-1.86 (m, 1H)

[1647] Example 60. N-((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrrolid in-3- yl)benzo[b]thiophene-2-sulfonamide

[1648] The title compound (8.7 mg) was prepared in the same fashion as Example 59, except that benzo[b]thiophene-2-sulfonyl chloride (34.6 mg, 0.15 mmol) was used instead of benzofuran 2- sulfonyl chloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.87-7.99 (m, 3H), 7.46-7.58 (m, 2H), 4.73-4.82 (m, 1H), 4.03-4.12 (m, 1H), 3.57-3.62 (m, 1H), 3.24-3.42 (m, 2H), 3.09-3.21 (m, 1H), 2.53-2.88 (m, 2H), 2.41-2.51 (m, 1H), 2.09-2.31 (m, 6H), 1.67-1.85 (m, 1H)

[1649] Example 61. (S)-1-(2-((S)-3-(quinolin-4-yloxy)pyrrolidin-1-yl)acetyl)pyr rolidine-2- carbonitrile

[1650] (S)-1-(2-((R)-3-Hydroxypyrrolidin-1-yl)acetyl)pyrrolidine-2- carbonitrile (242 mg, 1.08 mmol) prepared in Reference Example 26 was dissolved in tetrahydrofuran (9.03 ml). To the resulting solution, 4-quinolinol (157.34 mg, 1.08 mmol) and triphenylphosphine (568.6 mg, 2.17 mmol) were added. The mixture solution was cooled to 0°C. And then, DIAD (diisopropyl azodicarboxylate) (0.43 mL, 2.17 mmol) was slowly added dropwise for 15 minutes. The reaction mixture was stirred for 12 hours at room temperature. After the completion of the reaction, a saturated ammonium chloride solution and a saturated sodium hydrogen carbonate solution were added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with ethyl acetate. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol= 10/1, v/v) to give the title compound (18.5 mg). ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.73 (d, 1H), 8.19 (t, 1H), 8.04 (d, 1H), 7.70 (t, 1H), 7.51 (m, 1H), 6.64 (d, 1H), 5.16-5.09 (m, 1.3H), 4.76 (d, 0.7H), 3.71-3.63 (m, 2H), 3.53- 3.51 (m, 1H), 3.41-3.37 (m, 1H), 3.15-2.72 (m, 3H), 2.46-2.29 (m, 1H), 2.27-1.98 (m, 6H)

[1651] Example 62. (S)-1-(2-((S)-3-(naphthalen-1-yloxy)pyrrolidin-1-yl)acetyl)p yrrolidine-2- carbonitrile

[1652] The title compound (68 mg) was prepared in the same fashion as Example 61, except that naphthalen-1-ol (160 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) d 8.23 (t, 1H), 7.80 (d, 1H), 7.46-7.40 (m, 3H), 7.32 (t, 1H), 6.72 (d, 1H), 5.27 (d, 0.3H), 5.05 (m, 1H), 4.76 (m, 0.7H), 3.72-3.31 (m, 5H), 3.05-3.03 (m, 1H), 2.95-2.91 (m, 2H), 2.41-2.38 (m, 1H), 2.25-2.14 (m, 4H), 2.02-1.98 (m, 1H), 1.89 (m, 1H)

[1653] Example 63. (S)-1-(2-((S)-3-(quinolin-6-yloxy)pyrrolidin-1-yl)acetyl)pyr rolidine-2- carbonitrile

[1654] The title compound (87 mg) was prepared in the same fashion as Example 61, except that 6-quinolinol (160 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.76 (s, 1H), 8.04-7.98 (m, 2H), 7.35-7.27 (m, 2H), 6.97 (s, 1H), 5.24-4.76 (m, 2H), 3.68-3.34 (m, 5H), 3.01-2.89 (m, 3H), 2.44-2.10 (m, 7H)

[1655] Example 64. (S)-1-(2-((S)-3-(quinolin-8-yloxy)pyrrolidin-1-yl)acetyl)pyr rolidine-2- carbonitrile

[1656] The title compound (57 mg) was prepared in the same fashion as Example 61, except that 8-quinolinol (160 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.93 (s, 1H), 8.14 (d, 1H), 7.44-7.38 (m, 3H), 7.00-6.96 (m, 1H), 5.47 (d, 0.4H), 5.14 (s, 1H), 4.75 (d, 0.6H), 3.79-3.66 (m, 2H), 3.64-3.30 (m, 3H), 3.09-2.84 (m, 3H), 2.47-2.43 (m, 1H), 2.28-2.03 (m,

6H)

[1657] Example 65. (S)-1-(2-((S)-3-(isoquinolin-5-yloxy)pyrrolidin-1-yl)acetyl) pyrrolidine-2- carbonitrile

[1658] The title compound (62 mg) was prepared in the same fashion as Example 61, except that 5-isoquinolinol (160 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) d 9.20 (s, 1H), 8.51 (s, 1H), 8.00-7.99 (m, 1H), 7.54-7.46 (m, 2H), 6.91 (d, 1H), 5.19 (d, 0.3H), 5.05 (s, 1H), 4.76 (d, 0.7H), 3.71-3.63 (m, 2H), 3.53-3.51 (m, 1H), 3.42-3.38 (m, 2H), 3.13-2.91 (m, 3H), 2.48-2.41 (m, 1H), 2.34-1.99 (m, 6H)

[1659] Example 66. (S)-1-(2-((S)-3-(quinolin-5-yloxy)pyrrolidin-1-yl)acetyl)pyr rolidine-2- carbonitrile

[1660] The title compound (89 mg) was prepared in the same fashion as Example 61, except that 5-quinolinol (160 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.90 (s, 1H), 8.59 (t, 1H), 7.69 (d, 1H), 7.58 (t, 1H), 7.37 (s, 1H), 6.76 (d, 1H), 5.15 (m, 0.3H), 5.05 (m, 1H), 4.76 (m, 0.7H), 3.71-3.61 (m, 2H), 3.50 (m, 1H), 3.41-3.37 (m, 1H), 3.00-2.86 (m,3H), 2.45- 2.40 (m, 1H), 2.28-2.07 (m, 6H)

[1661] Example 67. (S)-1-(2-((S)-3-(benzo[d][1,3]dioxol-5-yloxy)pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1662] The title compound (76 mg) was prepared in the same fashion as Example 61, except that benzo[d][1,3]dioxol-5-ol (150 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 6.69 (d, 1H), 6.44 (d, 1H), 6.24 (t, 1H), 5.90 (s, 2H), 5.28 (d, 0.3H), 4.94-4.92 (m, 1H), 4.75 (m, 0.7H), 3.72-3.63 (m, 2H), 3.52-3.50 (m, 1H), 3.37-3.21 (m, 1H), 2.94-2.74 (m,3H), 2.33-2.00 (m, 7H)

[1663] Example 68. (S)-1-(2-((S)-3-(thieno[2,3-d]pyrimidin-4-yloxy)pyrrolidin-1 - yl)acetyl)pyrrolidine-2-carbonitrile

[1664] The title compound (63 mg) was prepared in the same fashion as Example 61, except that thieno[2,3-d]pyrimidin-4-ol (160 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.62 (s, 1H), 7.38 (s, 2H), 5.72 (m, 1H), 5.20 (d, 0.3H), 4.76 (m, 0.7H), 3.71-3.62 (m, 2H), 3.53-3.52 (m, 1H), 3.45-3.35 (m, 1H), 3.27-3.22 (m, 1H), 3.04 (d, 2H), 2.49-2.44 (m, 1H), 2.42-2.04 (m, 6H)

[1665] Example 69. (S)-1-(2-((S)-3-(isoquinolin-3-yloxy)pyrrolidin-1-yl)acetyl) pyrrolidine-2- carbonitrile

[1666] The title compound (123 mg) was prepared in the same fashion as Example 61, except that 3-isoquinolinol (151 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) d 8.89 (s, 1H), 7.84 (d, 1H), 7.65 (d, 1H), 7.54 (m, 1H), 7.34 (m, 1H), 6.96 (m, 1H), 5.47 (m, 1H),

4.71 (m, 1H), 3.68-3.37 (m, 4H), 3.07-2.89 (m, 3H), 2.41-2.01 (m, 7H)

[1667] Example 70. (S)-1-(2-((S)-3-((3-bromothieno[3,2-c]pyridin-4-yl)oxy)pyrro lidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1668] The title compound (70.2 mg) was prepared in the same fashion as Example 61, except that 3-bromothieno[3,2-c]pyridin-4-ol (151 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H- NMR (CDCI ₃ , 400MHz) δ 7.92 (s, 1H), 7.34 (m, 2H), 5.62 (m, 1H), 4.75 (m, 1H), 3.72-3.39 (m, 4H), 3.04-2.91 (m, 3H), 2.42 (m, 1H), 2.27-2.19 (m, 6H)

[1669] Example 71. (S)-1-(2-((S)-3-((4-chloronaphthalen-1-yl)oxy)pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1670] The title compound (94.0 mg) was prepared in the same fashion as Example 61, except that 4-chloronaphthalen-1-ol (194 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.27 (d, 1H), 8.16 (d, 1H), 7.58 (m, 1H), 7.52 (m, 1H), 7.40 (d, 1H), 6.60 (d, 1H), 4.90 (m, 1H), 4.70 (m, 1H), 3.61-3.27 (m, 4H), 2.97-2.69 (m, 3H), 2.39-1.93 (m, 7H)

[1671] Example 72. (S)-1-(2-((S)-3-(2-(1H-pyrazol-3-yl)phenoxy)pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1672] The title compound (81 mg) was prepared in the same fashion as Example 61 , except that 2-(1H-pyrazol-3-yl)phenol (173 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.63 (d, 1H), 7.56 (s, 1H), 7.25 (m, 1H), 7.04 (m, 1H), 6.97 (d, 1H), 6.58 (s, 1H), 5.03 (s, 1H), 4.72 (s, 1H), 3.59 (d, 2H), 3.44-3.40 (m, 2H), 3.22-3.02 (m, 3H), 2.31-2.30 (m, 1H), 2.20-1.98 (m, 6H)

[1673] Example 73. (S)-1-(2-((S)-3-((4-chloroquinazolin-8-yl)oxy)pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1674] The title compound (72 mg) was prepared in the same fashion as Example 61, except that 4-chloroquinazolin-8-ol (194 mg, 1.08 mmol) was used instead of 4-quinolinol of Example 59. ¹ H- NMR (CDCI ₃ , 400MHz) δ 7.85-7.47 (m, 3H), 7.20 (m, 1H), 5.03 (s, 1H), 4.76 (s, 1H), 3.66-3.54 (m, 4H), 3.18-2.96 (m, 4H), 2.49-2.05 (m, 6H)

[1675] Example 74. (S)-1-(2-((S)-3-((4-chloroquinazolin-6-yl)oxy)pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1676] The title compound (90.7 mg) was prepared in the same fashion as Example 61, except that 4-chloroquinazolin-6-ol (194 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.97 (m, 1H), 7.62-7.54 (m, 2H), 7.37 (d, 1H), 5.06 (s, 1H), 4.75 (s, 1H), 3.69- 3.36 (m, 4H), 3.10-2.92 (m, 4H), 2.49-2.18 (m, 6H)

[1677] Example 75. (S)-1-(2-((S)-3-((2-oxo-1,2-dihydroquinolin-5-yl)oxy)pyrroli din-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1678] The title compound (93.9 mg) was prepared in the same fashion as Example 61, except that 5-hydroxyquinolin-2(lH)-one (176 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 11.68 (m, 1H), 8.17 (m, 1H), 7.37-7.26 (m, 1H), 6.61 (m, 1H), 6.49 (m, 1H), 5.10 (s, 2H), 3.70-3.30 (m, 4H), 2.96-2.85 (m, 3H), 2.38-1.92 (m, 7H)

[1679] Example 76. (S)-1-(2-((S)-3-((2-oxo-1,2,3,4-tetrahydroquinolin-5-yl)oxy) pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1680] The title compound (112.3 mg) was prepared in the same fashion as Example 61, except that 5-hydroxy-3,4-dihydroquinolin-2(1H)-one (176 mg, 1.08 mmol) was used instead of 4- quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 9.71 (s, 0.5H), 8.49 (s, 0.5H), 7.07 (t, 1H), 6.44 (m, 2H), 4.87 (s, 1H), 4.76 (s, 1H), 3.69-3.30 (m, 4H), 2.92-2.79 (m, 5H), 2.70-2.57 (m, 2H), 2.33-2.06 (m,

7H)

[1681] Example 77. (S)-1-(2-((S)-3-((2-oxo-1,2,3,4-tetrahydroquinolin-6-yl)oxy) pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1682] The title compound (94.0 mg) was prepared in the same fashion as Example 61, except that 6-hydroxy-3,4-dihydroquinolin-2(lH)-one (176 mg, 1.08 mmol) was used instead of 4- quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 9.02 (s, 1H), 6.76 (d, 1H), 6.64 (m, 2H), 4.79-4.75 (m, 2H), 3.68-3.24 (m, 5H), 2.90-2.59 (m, 7H), 2.27-2.02 (m, 6H)

[1683] Example 78. (S)-1-(2-((S)-3-((2-methylquinolin-8-yl)oxy)pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1684] The title compound (74.5 mg) was prepared in the same fashion as Example 61, except that 2-methylquinolin-8-ol (171.7 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.28 (s, 1H), 7.52 (s, 1H), 7.34 (d, 2H), 6.89 (d, 1H), 5.09 (s, 1H), 4.98 (s, 1H),

3.78-3.53 (m, 4H), 3.42-3.16 (m, 3H), 2.80-2.75 (m, 4H), 2.43-2.04 (m, 6H)

[1685] Example 79. (S)-1-(2-((S)-3-((7-chloroquinolin-4-yl)oxy)pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1686] The title compound (50.7 mg) was prepared in the same fashion as Example 61, except that 7-chloroquinolin-4-ol (194 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.69 (s, 1H), 8.13 (d, 1H), 8.00 (s, 1H), 7.43 (d, 1H), 6.62 (s, 1H), 5.07 (s, 1.3H), 4.75 (s, 0.7H), 3.69-3.27 (m, 4H), 3.09-2.86 (m, 3H), 2.46-2.45 (m, 1H), 2.28-2.00 (m, 6H)

[1687] Example 80. (S)-1-(2-((S)-3-((7-bromoquinolin-4-yl)oxy)pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1688] The title compound (48.6 mg) was prepared in the same fashion as Example 61, except that 7-bromoquinolin-4-ol (237 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.67 (s, 1H), 8.17 (s, 1H), 8.05 (d, 1H), 7.55 (d, 1H), 6.63 (s, 1H), 5.06 (s, 1.3H), 4.75 (s, 0.7H), 3.71-3.26 (m, 4H), 3.03-2.85 (m, 3H), 2.45-2.44 (m, 1H), 2.31-1.99 (m, 6H)

[1689] Example 81. (S)-1-(2-((S)-3-((5-chloroquinolin-8-yl)oxy)pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1690] The title compound (41 mg) was prepared in the same fashion as Example 61, except that 5-chloroquinolin-8-ol (194 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.95 (s, 1H), 8.53 (d, 1H), 7.53-7.49 (m, 2H), 6.92 (d, 1H), 5.11 (s, 1H), 4.75 (s, 1H),

3.79-3.35 (m, 5H), 3.16-2.93 (m, 2H), 2.45 (m, 1H), 2.22-2.04 (m, 6H)

[1691] Example 82. (S)- 1 -(2-((S)-3-([ 1 , 1 '-biphenyl] -3 -yloxy)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2- carbonitrile

[1692] The title compound (81 mg) was prepared in the same fashion as Example 61 , except that [1,1'-biphenyl]-3-ol (184 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.56-7.48 (m, 2H), 7.42-7.27 (m, 4H), 7.17-7.06 (m, 2H), 6.90-6.67 (m, 1H), 4.91 (s, 1H), 4.72 (s, 1H), 3.74-3.27 (m, 5H), 2.88-2.83 (m, 2H), 2.31 (m, 1H), 2.30-2.11 (m, 6H)

[1693] Example 83. (S)- 1 -(2-((S)-3-([ 1,1'-biphenyl]-4-yloxy)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2- carbonitrile

[1694] The title compound (63.7 mg) was prepared in the same fashion as Example 61, except that [1,1'-biphenyl]-4-ol (184 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.53-7.48 (m, 4H), 7.39 (t, 2H), 7.28 (t, 1H), 6.90 (d, 2H), 4.88 (s, 1H), 4.72 (s, 1H), 3.74- 3.28 (m, 5H), 2.87-2.80 (m, 2H), 2.31 (m, 1H), 2.12-1.97 (m, 6H)

[1695] Example 84. (S)-1-(2-((S)-3-((6-methoxyquinolin-4-yl)oxy)pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1696] The title compound (70.2 mg) was prepared in the same fashion as Example 61, except that 6-methoxyquinolin-4-ol (183 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.57 (s, 1H), 7.94 (d, 1H), 7.43 (s, 1H), 7.34 (d, 1H), 6.60 (s, 1H), 5.06 (s, 1.4H), 4.75 (s, 0.6H), 3.93 (s, 3H), 3.71-3.69 (m, 2H), 3.63-3.25 (m, 3H), 3.04-2.84 (m, 2H), 2.45 (m, 1H), 2.27-1.98 (m, 6H)

[1697] Example 85. (S)-1-(2-((S)-3-((7,8-difluoroquinolin-4-yl)oxy)pyrrolidin-1 - yl)acetyl)pyrrolidine-2-carbonitrile

[1698] The title compound (62.6 mg) was prepared in the same fashion as Example 61, except that 7,8-difluoroquinolin-4-ol (194 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.75 (s, 1H), 7.94 (s, 1H), 7.33 (d, 1H), 6.67 (s, 1H), 5.09 (s, 1.4H), 4.76 (s, 0.6H), 3.93 (s, 3H), 3.71-3.66 (m, 2H), 3.56-3.27 (m, 3H), 3.06-2.86 (m, 2H), 2.48-2.45 (m, 1H), 2.29-2.00 (m, 6H)

[1699] Example 86. Methyl 6-(((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrroli din-3- yl)oxy)-2,3-dihydrobenzofuran-2-carboxylate

[1700] The title compound (85.3 mg) was prepared in the same fashion as Example 61, except that methyl 6-hydroxy-2,3-dihydrobenzofuran-2-carboxylate (205 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.02 (d, 1H), 6.41-6.36 (m, 2H), 5.23 (t, 1H), 4.80 (s, 1H), 4.72 (s, 1H), 4.08 (m, 1H), 3.80 (s, 3H), 3.74-3.65 (m, 2H), 3.51-3.39 (m, 3H), 3.33-3.25 (m,

2H), 2.91-2.86 (m, 2H), 2.25-2.04 (m, 6H)

[1701] Example 87. (S)-1-(2-((S)-3-(4-phenoxyphenoxy)pyrrolidin-1-yl)acetyl)pyr rolidine-2- carbonitrile

[1702] The title compound (50.7 mg) was prepared in the same fashion as Example 61, except that 4-phenoxyphenol (205 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.29-2.27 (m, 2H), 7.03 (s, 1H), 6.94 (s, 4H), 6.82 (d, 2H), 4.81-4.69 (m, 2H), 3.74-3.62 (m, 2H), 3.51-3.43 (m, 1H), 3.39-3.35 (m, 1H), 3.32-3.24 (m, 1H), 2.87-2.82 (t, 2H), 2.29-2.04 (m, 7H)

[1703] Example 88. (S)-1-(2-((S)-3-(3-phenoxyphenoxy)pyrrohdin-1-yl)acetyl)pyrr ohdine-2- carbonitrile

[1704] The title compound (95 mg) was prepared in the same fashion as Example 61, except that 3-phenoxyphenol (205 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.34 (t, 2H), 7.19 (t, 1H), 7.13 (t, 1H), 7.02 (d, 2H), 6.57 (d, 2H), 6.49 (s, 1H), 4.81 (s, 1H), 4.74 (s, 1H), 3.71-3.63 (m, 1H), 3.51-3.49 (m, 1H), 3.38-3.23 (m, 2H), 2.86-2.62 (m, 3H), 2.27- 2.02 (m, 7H)

[1705] Example 89. (S)-1-(2-((S)-3-(4-benzylphenoxy)pyrrolidin-1-yl)acetyl)pyrr olidine-2- carbonitrile

[1706] The title compound (96.1 mg) was prepared in the same fashion as Example 61, except that 4-benzylphenol (194 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.26 (s, 2H), 7.17 (t, 3H), 7.08 (d, 2H), 6.76 (d, 2H), 4.81 (s, 1H), 4.74 (s, 1H), 3.90 (s, 2H), 3.73-3.67 (m, 2H), 3.51-3.27 (m, 2H), 2.85-2.76 (m, 3H), 2.26-2.02 (m, 7H)

[1707] Example 90. (S)-1-(2-((S)-3-(2-benzylphenoxy)pyrrolidin-1-yl)acetyl)pyrr olidine-2- carbonitrile

[1708] The title compound (69.1 mg) was prepared in the same fashion as Example 61, except that 2-benzylphenol (194 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.26-7.09 (m, 7H), 6.87 (t, 1H), 6.74 (d, 1H), 4.93 (s, 1H), 4.73 (s, 1H), 3.94 (s, 2H), 3.71-3.64 (m, 1H), 3.57-3.48 (m, 1H), 3.32-3.24 (m, 2H), 2.84-2.64 (m, 3H), 2.23-1.97 (m, 7H)

[1709] Example 91. (S)-1-(2-((S)-3-(3-benzoylphenoxy)pynOlidin-1-yl)acetyl)pyrr olidine-2- carbonitrile

[1710] The title compound (93.9 mg) was prepared in the same fashion as Example 61, except that 3-hydroxybenzophenone (216 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.79 (d, 2H), 7.59 (d, 1H), 7.50 (t, 2H), 7.37-7.27 (m, 3H), 7.09 (d, 1H), 4.93 (s, 1H), 4.47 (s, 1H), 3.73-3.65 (m, 2H), 3.56-3.30 (m, 2H), 2.97-2.86 (m, 3H), 2.34-1.98 (m, 7H)

[1711] Example 92. (S)-1-(2-((S)-3-(4-benzoylphenoxy)pyrrolidin-1-yl)acetyl)pyr rolidine-2- carbonitrile

[1712] The title compound (49.7 mg) was prepared in the same fashion as Example 61, except that 4-hydroxybenzophenone (216 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.80 (d, 2H), 7.74 (d, 2H), 7.56 (d, 1H), 7.48 (d, 2H), 6.91 (d, 2H), 4.97 (s, 1H), 4.73 (s, 1H), 3.75-3.65 (m, 2H), 3.57-3.40 (m, 2H), 3.00-2.88 (m, 3H), 2.38-2.36 (m, 1H), 2.18-1.99 (m, 6H)

[1713] Example 93. (S)-1-(2-((S)-3-((4-(benzyloxy)pyridin-2-yl)oxy)pyrrolidin-1 - yl)acetyl)pyrrolidine-2-carbonitrile

[1714] The title compound (95.0 mg) was prepared in the same fashion as Example 61, except that 4-(benzyloxy)pyridin-2-ol (216 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.92 (d, 1H), 7.38-7.34 (m, 6H), 6.51 (s, 1H), 5.43 (s, 1H), 5.03 (s, 2H), 4.72 (s, 1H), 3.72-3.44 (m, 2H), 3.38-3.28 (m, 2H), 3.17-3.13 (m, 1H), 2.96-2.87 (m, 1H), 2.80-2.71 (m, 1H), 2.35-2.30 (m, 1H), 2.19-1.97 (m, 6H)

[1715] Example 94. (S)-1-(2-((S)-3-((7-(trifluoromethyl)quinolin-4-yl)oxy)pyrro lidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1716] The title compound (97.2 mg) was prepared in the same fashion as Example 61, except that 7-(trifluoromethyl)quinolin-4-ol (227 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H- NMR (CDCI ₃ , 400MHz) δ 8.78 (s, 1H), 8.30 (s, 2H), 7.64 (d, 1H), 6.74 (d, 1H), 5.10 (s, 1H), 4.76 (s, 1H), 3.72-3.24 (m, 4H), 3.13-2.96 (m, 2H), 2.86 (m, 1H), 2.50-2.45 (m, 1H), 2.18-1.99 (m, 6H)

[1717] Example 95. (S)-1-(2-((S)-3-((8-(trifluoromethyl)quinolin-4-yl)oxy)pyrro lidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1718] The title compound (57.2 mg) was prepared in the same fashion as Example 61, except that 8-(trifluoromethyl)quinolin-4-ol (227 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H- NMR (CDC1 ₃ , 400MHz) δ 8.84 (d, 1H), 8.43 (d, 1H), 8.03 (d, 1H), 7.53 (t, 1H), 6.74 (s, 1H), 5.08 (s, 1H), 4.75 (s, 1H), 3.72-3.38 (m, 4H), 3.29-2.82 (m, 3H), 2.47-2.45 (m, 1H), 2.26-1.98 (m, 6H)

[1719] Example 96. (S)-1-(2-((S)-3-(acridin-4-yloxy)pyrrolidin-1-yl)acetyl)pyrr olidine-2- carbonitrile

[1720] The title compound (51.8 mg) was prepared in the same fashion as Example 61, except that acridin-4-ol (205 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) d 8.67 (s, 1H), 8.31 (d, 1H), 7.92 (d, 1H), 7.69 (m, 1H), 7.52-7.48 (t, 2H), 7.36 (t, 1H), 6.92 (d, 1H), 5.01 (s, 1H), 4.76 (s, 1H), 3.76-3.27 (m, 6H), 2.85 (m, 1H), 2.45 (m, 1H), 2.21-1.96 (m, 6H)

[1721] Example 97. (S)-1-(2-((S)-3-(2-(benzo[d]oxazol-2-yl)phenoxy)pyrrolidin-1 - yl)acetyl)pyrrolidine-2-carbonitrile

[1722] The title compound (71.3 mg) was prepared in the same fashion as Example 61, except that 2-(benzo[d]oxazol-2-yl)-phenol (227 mg, 1.08 mmol) was used instead of 4-quinolinol of Example 61. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.09 (d, 1H), 7.77 (s, 1H), 7.58 (s, 1H), 7.44 (t, 1H),

7.34 (m, 2H), 7.06 (t, 1H), 6.96 (d, 1H), 4.99 (s, 1H), 4.69 (s, 1H), 3.69-3.54 (m, 1H), 3.48-3.27 (m, 3H), 3.03-2.84 (m, 3H), 2.37-2.32 (m, 1H), 2.17-1.88 (m, 6H)

[1723] Example 98. (S)-1-(2-((S)-3-(4-(4-acetylpiperazin-1-yl)phenoxy)pyrrolidi n-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1724] The title compound (85.3 mg) was prepared in the same fashion as Example 61, except that 4-(4-acetylpiperazin-1-yl)phenol (238 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H- NMR (CDCI ₃ , 400MHz) δ 6.88 (d, 2H), 6.79 (d, 2H), 4.78 (s, 1.4H), 4.47 (s, 0.6H), 3.74-3.68 (m,

3H), 3.60-3.50 (m, 3H), 3.36-3.34 (m, 1H), 3.17-3.15 (m, 1H), 3.04-3.01 (m, 5H), 2.84-2.77 (m, 2H), 2.28-1.99 (m, 10H)

[1725] Example 99. 2-(((S)-1-(2-((S)-2-cyanopyrrolidin-1-yl)-2-oxoethyl)pyrroli din-3-yl)oxy)-N- pheny1benzamide

[1726] The title compound (40.0 mg) was prepared in the same fashion as Example 61, except that 2-hydroxy-N-pheny1benzamide (227 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H- NMR (CDCI ₃ , 400MHz) δ 10.06-9.94 (m, 1H), 8.21 (d, 1H), 7.73 (d, 2H), 7.41 (t, 1H), 7.33 (m, 2H), 7.09 (d, 2H), 6.91 (d, 1H), 5.06 (s, 1H), 4.58 (s, 1H), 3.61-3.26 (m, 4H), 3.07-2.99 (m, 2H), 2.69-2.67 (m, 1H), 2.40-2.38 (m, 1H), 2.10-1.80 (m, 6H)

[1727] Example 100. (S)-1-(2-((S)-3-((7-methoxyquinolin-4-yl)oxy)pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1728] The title compound (58.3 mg) was prepared in the same fashion as Example 61, except that 7-methoxyquinolin-4-ol (183 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.60 (s, 1H), 8.07 (d, 1H), 7.34 (s, 1H), 7.12 (d, 1H), 6.51 (s, 1H), 5.02 (m,

1.2H), 4.72 (s, 0.8H), 3.91 (s, 4H), 3.64-3.57 (m, 1H), 3.46-3.28 (m, 3H), 2.99-2.80 (m, 2H), 2.40 (m, 1H), 2.12-2.10 (m, 6H)

[1729] Example 101. (S)-1-(2-((S)-3-((6,7-dimethoxyquinohn-4-yl)oxy)pyrrolidin-1 - yl)acetyl)pyrrolidine-2-carbonitrile

[1730] The title compound (73.4 mg) was prepared in the same fashion as Example 61, except that 6,7-dimethoxyquinolin-4-ol (216 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.52 (d, 1H), 7.40 (s, 1H), 7.35 (s, 1H), 6.54 (s, 1H), 5.04-5.03 (m, 1H), 4.73 (s, 1H), 4.02 (d, 6H), 3.80-3.51 (m, 1H), 3.48-3.26 (m, 3H), 3.05-2.93 (m, 2H), 2.80-2.79 (m, 1H), 2.47- 2.42 (m, 1H), 2.24-1.99 (m, 6H)

[1731] Example 102. (S)-1-(2-((S)-3-((3-(4-bromophenyl)isoxazol-5-yl)oxy)pyrroli din-1- yl)acetyl)pyrrolidine-2-carbonitrile [1732] The title compound (79.9 mg) was prepared in the same fashion as Example 61, except that (3-(4-bromophenyl)isoxazol-5-ol (258 mg, 1.08 mmol) was used instead of 4-quinolinol. ¹ H- NMR (CDC1 ₃ , 400MHz) δ 7.61-7.50 (m, 5H), 5.04 (s, 1H), 4.74 (s, 1H), 3.65-3.61 (m, 1H), 3.50-3.41 (m, 2H), 3.17-2.86 (m, 4H), 2.38 (m, 1H), 2.26-2.08 (m, 6H)

[1733] Example 103. (S)-4,4-difluoro-1-(2-((S)-3-((6-methoxyquinolin-4-yl)oxy)py rrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1734] (S)-4,4-Difluoro-1-(2-((R)-3-hydroxypyrrolidin-1-yl)-2-oxoet hyl)pyrrolidine-2- carbonitrile(260 mg, 1.00 mmol) prepared in Reference Example 27 was dissolved in tetrahydrofuran (10 ml). To the resulting solution, 6-methoxyquinolin-4-ol (180 mg, 1.0 mmol) and triphenylphosphine (520 mg, 2.0 mmol) were added. The mixture solution was cooled to 0°C. And then, DIAD (diisopropyl azodicarboxylate) (0.39 mL, 2.0 mmol) was slowly added dropwise for 15 minutes. The reaction mixture was stirred for 12 hours at room temperature. After the completion of the reaction, a saturated ammonium chloride solution and a saturated sodium hydrogen carbonate solution were added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with ethyl acetate. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol= 10/1, v/v) to give the title compound (45 mg). ¹ H- NMR (CDC1 ₃ , 400MHz) δ 8.54 (d, 1H), 7.92 (d, 1H), 7.43-7.38 (m, 1H), 7.33-7.26 (m, 1H), 6.55 (s, 1H), 4.99-4.95 (m, 2H), 4.13-.98 (m, 1H), 3.91-3.85 (m, 4H), 3.70-3.58 (m, 1H), 3.50-3.35 (m, 1H), 3.11-2.96 (m, 3H), 2.70-2.66 (m, 3H), 2.44-.40 (m, 1H), 2.12-2.09 (m 1H)

[1735] Example 104. (S)-4,4-difluoro-1-(2-((S)-3-((8-(trifluoromethyl)quinolin-4 - yl)oxy)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2-carbonitrile

[1736] The title compound (87 mg) was prepared in the same fashion as Example 103, except that 8-(trifluoromethyl)quinolin-4-ol (210 mg, 1.0 mmol) was used instead of 6-methoxyquinolin-4-ol. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.82 (s, 1H), 8.41 (d, 1H), 8.02 (d, 1H), 6.71 (s, 1H), 5.11-4.98 (m,

2H), 4.16-4.06 (m, 2H), 3.65-3.42 (m, 2H), 3.16-2.99 (m, 3H), 2.73-2.72 (m, 3H), 2.43 (m, 1H), 2.12- 2.01 (m, 1H)

[1737] Example 105. (S)-4,4-difluoro-1-(2-((S)-3-(quinohn-5-yloxy)pyrrolidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1738] The title compound (68 mg) was prepared in the same fashion as Example 103, except that quinolin-5-ol (150 mg, 1.0 mmol) was used instead of 6-methoxyquinolin-4-ol. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.87 (s, 1H), 8.55 (d, 1H), 7.68 (d, 1H), 7.56 (t, 1H), 7.36 (s, 1H), 6.73 (d, 1H), 4.97 (m, 2H), 4.13-3.97 (m, 2H), 3.68-3.36 (m, 2H), 3.09 (m, 1H), 2.99-2.91 (m, 2H), 2.68 (m, 3H), 2.39-2.36 (m, 1H), 2.21-2.03 (m, 1H) [1739] Example 106. (S)-4,4-difluoro-1-(2-((S)-3-(isoquinolin-5-yloxy)pyrrolidin -1- yl)acetyl)pyrrolidine-2-carbonitrile

[1740] The title compound (59 mg) was prepared in the same fashion as Example 103, except that isoquinolin-5-ol (150 mg, 1.0 mmol) was used instead of 6-methoxyquinolin-4-ol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 9.17 (s, 1H), 8.49 (d, 1H), 7.98 (d, 1H), 7.50-7.44 (m, 2H), 6.89 (d, 1H), 4.99 (m, 2H), 4.12-3.69 (m, 2H), 3.44-3.39 (m, 2H), 3.16-2.93 (m, 3H), 2.72-2.70 (m, 3H), 2.41-2.38 (m, 1H), 2.24- 2.13 (m, 1H)

[1741] Example 107. (S)-1-(2-((S)-3-((7-chloroquinolin-4-yl)oxy)pyrrolidin-1-yl) acetyl)-4,4- difluoropyrrolidine-2-carbonitrile

[1742] The title compound (37 mg) was prepared in the same fashion as Example 103, except that 7-chloroquinolin-4-ol (180 mg, 1.0 mmol) was used instead of 6-methoxyquinolin-4-ol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.65 (s, 1H), 8.06 (d, 1H), 7.96 (s, 1H), 7.47-7.31 (m, 1H), 6.58 (d, 1H), 5.01- 4.97 (m, 2H), 4.17-3.99 (m, 2H), 3.86-3.35 (m, 2H), 3.12-3.11 (m, 1H), 3.02-2.97 (m, 2H), 2.77-2.71 (m, 3H), 2.42-2.41 (m, 1H), 2.12-2.10 (m, 1H)

[1743] Example 108. (S)-4,4-difluoro-1-(2-((S)-3-((2-oxo-1,2-dihydroquinolin-5- yl)oxy)pyrrolidin- 1 -yl)acetyl)pyrrolidine-2-carbonitrile

[1744] The title compound (64 mg) was prepared in the same fashion as Example 103, except that 5-hydroxyquinolin-2(lH)-one (160 mg, 1.0 mmol) was used instead of 6-methoxyquinolin-4-ol. ¹ H- NMR (CDC1 ₃ , 400MHz) δ 12.24 (s, 1H), 8.18 (d, 1H), 7.37 (m, 1H), 7.01 (s, 1H), 6.62 (d, 1H), 6.50 (d, 1H), 4.97 (s, 2H), 4.15-4.00 (m, 2H), 3.39 (m, 2H), 3.12-3.03 (m, 3H), 2.92-2.72 (m, 3H), 2.53- 2.36 (m, 1H), 2.09 (m, 1H)

[1745] Example 109. (S)-1-(2-((S)-3-((5-chloroquinolin-8-yl)oxy)pyrrolidin-1-yl) acetyl)-4,4- difluoropyrrolidine-2-carbonitrile

[1746] The title compound (81 mg) was prepared in the same fashion as Example 103, except that 5-chloroquinolin-8-ol (180 mg, 1.0 mmol) was used instead of 6-methoxyquinolin-4-ol. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.91 (s, 1H), 8.50 (d, 1H), 7.52-7.46 (m, 2H), 6.86 (d, 1H), 5.06-4.99 (m, 2H), 4.10-3.84 (m, 3H), 3.51-3.32 (m, 1H), 3.09-3.07 (m, 3H), 2.87-2.57 (m, 3H), 2.39 (m, 1H), 2.21 (m, 1H)

[1747] Example 110. (S)-1-(2-(4-(quinolin-5-ylamino)piperidin-1-yl)acetyl)pyrrol idine-2- carbonitrile

[1748] (S)-1-(2-Chloroacetyl)pyrrolidine-2-carbonitrile (28.75 mg, 0.16 mmol) prepared in

Reference Example 1 was dissolved in anhydrous dichloromethane (4 ml). To the resulting solution,

N-(piperidin-4-yl)quinolin-5-amine hydrochloride (50.0 mg, 0.16 mmol) prepared in Reference Example 29 and potassium carbonate (115.0 mg, 0.83 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography

(dichloromethane/methanol= 10/1, v/v) to give the title compound (12 mg). ¹ H-NMR (CDCft, 400 MHz) d 8.83 (d, 1H), 8.25 (d, 1H), 7.54-7.42 (m, 2H), 7.28 (s, 1H), 6.52 (d, 1H), 5.15 (d, 0.3H), 4.80 (s, 0.7H), 4.12 (d, 1H), 3.69-2.90 (m, 8H), 2.37-2.04 (m, 8H), 1.79-1.64 (m, 2H)

[1749] Example 111. (S)-l -(2-(4-(quinolin-4-ylamino)piperidin- 1 -yl)acetyl)pyrrolidine-2- carbonitrile

[1750] The title compound (19.8 mg) was prepared in the same fashion as Example 110, except thatN-(piperidin-4-yl)quinolin-4-amine hydrochloride (50.0 mg, 0.16 mmol) prepared in Reference Example 28 was used instead of N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CD ₃ OD, 400MHz) δ 8.35-8.32 (m, 2H), 7.81 (s, 2H), 7.59 (s, 1H), 6.79 (d, 1H), 4.80 (s, 1H), 3.78 (s, 2H), 3.60-3.35 (m, 2H), 3.08 (d, 2H), 2.42-1.82 (m, 11H), 1.28 (s, 1H)

[1751] Example 112. (1S,3S,5S)-2-(2-(4-(quinolin-4-ylamino)piperidin-1-yl)acetyl )-2- azabicyclo[3.1.0]hexane-3-carbonitrile

[1752] (1S,3S,5S)-2-(2-Chloroacetyl)-2-azabicyclo[3.1.0]hexane-3-ca rbonitrile (25 mg, 0.14 mmol) prepared in Reference Example 4 was dissolved in anhydrous acetonitrile (3.5 ml). To the resulting solution, N-(piperidin-4-yl)quinolin-4-amine hydrochloride (50.0 mg, 0.16 mmol) prepared in Reference Example 28 and potassium carbonate (115.0 mg, 0.83 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol= 10/1, v/v) to give the title compound (11.9 mg). ¹ H- NMR (CD ₃ OD, 400 MHz) δ 8.39 (d, 2H), 7.84 (s, 2H), 7.64 (s, 1H), 6.88 (s, 1H), 5.04 (s, 1H), 3.84 (s, 2H), 3.57-3.46 (m, 2H), 3.10 (s, 2H), 2.63 (s, 1H), 2.43 (d, 2H), 2.30 (d, 1H), 2.03-2.18 (m, 1H), 2.10-1.75 (m, 4H), 1.25-0.92 (s, 3H)

[1753] Example 113. (2S,4S)-4-fluoro-1-(2-(4-(quinolin-5-ylamino)piperidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1754] The title compound (29.9 mg) was prepared in the same fashion as Example 110, except that (2S,4S)-1-(2-chloroacetyl)-4-fluoro-pyrrolidine-2-carbonitri le (25.4 mg, 0.14 mmol) prepared in

Reference Example 2 was used instead of (S)-1-(2-chloroacetyl)pyrrolidine-2-carbonitrile. ¹ H-NMR (CD ₃ OD, 400 MHz) δ 8.72 (s, 1H), 8.63 (d, 1H), 7.56 (t, 1H), 7.42 (s, 1H), 7.28 (d, 1H), 6.73 (d, 1H), 5.45 (d, 1H), 4.99 (d, 1H), 3.91-3.78 (m, 1H), 3.61-3.43 (m, 3H), 3.16-3.08 (m, 2H), 2.61-2.45 (m, 4H), 2.18 (q, 2H), 2.01-1.78 (m, 4H)

[1755] Example 114. (S)-4,4-difluoro-1-(2-(4-(quinolin-5-ylamino)piperidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1756] The title compound (12.6 mg) was prepared in the same fashion as Example 110, except that (2S)-1-(2-chloroacetyl)-4,4-difluoro-pyrrolidine-2-carbonitr ile (27.8 mg, 0.14 mmol) prepared in

Reference Example 3 was used instead of (S)-1-(2-chloroacetyl)pyrrolidine-2-carbonitrile. ¹ H-NMR (CD ₃ OD, 400 MHz) δ 8.72 (d, 1H), 8.62 (d, 1H), 7.54 (t, 1H), 7.40 (d, 1H), 7.27 (d, 1H), 6.71 (d, 1H), 5.58-5.05 (m, 1H), 4.25-4.09 (m, 2H), 3.53-3.24 (m, 3H), 3.00 (d, 2H), 2.90-2.74 (m, 2H), 2.39 (t,

2H), 2.13 (d, 2H), 1.72 (d, 2H), 1.28 (s, 1H)

[1757] Example 115. (1S,3S,5S)-2-(2-(4-(quinolin-5-ylamino)piperidin-1-yl)acetyl )-2- azabicyclo[3.1 0]hexane-3-carbonitrile

[1758] The title compound (33.6 mg) was prepared in the same fashion as Example 112, except thatN-(piperidin-4-yl)quinolin-5-amine hydrochloride (50.0 mg, 0.16 mmol) prepared in Reference Example 29 was used instead of N-(piperidin-4-yl)quinolin-4-amine hydrochloride. ¹ H-NMR (CD ₃ OD, 400 MHz) δ 8.72 (s, 1H), 8.66 (d, 1H), 7.56 (t, 1H), 7.41 (d, 1H), 7.29 (d, 1H), 6.73 (d, 1H), 5.56-5.05 (m, 1H), 3.81 (s, 1H), 3.77 (s, 1H), 3.65 (d, 2H), 3.22-3.06 (m, 2H), 2.64 (t, 3H), 2.47-1.81 (m, 7H), 1.28-1.07 (m, 2H)

[1759] Example 116. (S)-1-(2-(4-(furo[3,2-c]pyridin-7-ylamino)piperidin-1-yl)ace tyl)pyrrolidine-

2-carbonitrile

[1760] The title compound (21.6 mg) was prepared in the same fashion as Example 110, except thatN-(piperidin-4-yl)furo[3,2-c]pyridin-7-amine hydrochloride (40.6 g, 0.16 mmol) prepared in

Reference Example 30 was used instead of N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H- NMR (CDCI ₃ , 400 MHz) δ 8.35 (s, 1H), 7.91 (s, 1H), 7.61 (s, 1H), 6.81 (s, 1H), 4.76 (s, 1H), 3.94 (s, 1H), 3.74 (s, 1H), 3.57 (d, 2H), 3.43-3.36 (m, 1H), 3.24 (s, 1H), 2.84-3.03 (m, 2H), 2.44-2.14 (m, 7H), 1.87-1.52 (m, 2H)

[1761] Example 117. (S)-1-(2-(4-(quinolin-3-yloxy)piperidin-1-yl)acetyl)pyrrolid ine-2- carbonitrile

[1762] (S)-1-(2-Chloroacetyl)pyrrolidine-2-carbonitrile (60 mg, 0.35 mmol) prepared in

Reference Example 1 was dissolved in anhydrous dichloromethane (2.4 ml). To the resulting solution,

3-(piperidin-4-yloxy)quinoline hydrochloride (104.7 mg, 0.35 mmol) prepared in Reference Example 31 and potassium carbonate (192.2 mg, 1.39 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol= 10/1, v/v) to give the title compound (22.1 mg). ¹ H-NMR (CDCft, 400 MHz 8).6 δ6 (d, 1H), 8.04 (d, 1H), 7.71 (d, 1H), 7.57-7.49 (m, 2H) 7.40 (s, 1H), 5.20 (d, 0.3H), 4.76 (d, 0.6H), 4.52 (s, 1H), 3.75-3.54 (m, 2H), 3.44-3.24 (m, 2H) 2. 82-2.51 (m, 5H) 2.28-1.94 (m, 8H)

[1763] Example 118. (S)-1-(2-(4-(quinolin-4-yloxy)piperidin-1-yl)acetyl)pyrrolid ine-2- carbonitrile

[1764] The title compound (23.6 mg) was prepared in the same fashion as Example 117, except that 4-(piperidin-4-yloxy)quinoline hydrochloride (104.7 mg, 0.35 mmol) prepared in Reference Example 32 was used instead of 3-(piperidin-4-yloxy)quinoline hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.72 (d, 1H), 8.23 (d, 1H), 8.03 (d, 1H), 7.70 (t, 1H), 7.52 (t, 1H), 6.72 (d, 1H) 5.17 (d, 0.3H), 4.76 (d, 0.6H), 4.69 (s, 1H), 3.75-3.55 (m, 2H), 3.43-3.24 (m, 2H) 2. 93-2.36 (m, 5H) 2.28- 2.05 (m, 8H)

[1765] Example 119. (S)-1-(2-(4-(quinolin-5-yloxy)piperidin-1-yl)acetyl)pyrrolid ine-2- carbonitrile

[1766] The title compound (20.2 mg) was prepared in the same fashion as Example 117, except that 5-(piperidin-4-yloxy)quinoline hydrochloride (104.7 mg, 0.35 mmol) prepared in Reference Example 33 was used instead of 3-(piperidin-4-yloxy)quinoline hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.90 (s, 1H), 8.61 (d, 1H), 7.68 (d, 1H), 7.60 (t, 1H), 7.39 (q, 1H), 6.88 (d, 1H) 5.21 (d, 0.3H), 4.76 (s, 0.6H), 4.61 (s, 1H), 3.76-3.54 (m, 2H), 3.44-3.23 (m, 2H) 2. 83-2.58 (m, 5H) 2.28-2.01 (m, 8H)

[1767] Example 120. (S)-1-(2-(4-(quinolin-6-yloxy)piperidin-1-yl)acetyl)pyrrolid ine-2- carbonitrile

[1768] The title compound (24.4 mg) was prepared in the same fashion as Example 117, except that 6-(piperidin-4-yloxy)quinoline hydrochloride (104.7 mg, 0.35 mmol) prepared in Reference Example 34 was used instead of 3-(piperidin-4-yloxy)quinoline hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.76 (d, 1H), 8.05-8.00 (m, 2H), 7.38-7.34 (m, 2H), 7.10 (s, 1H), 5.24 (d, 0.3H), 4.76 (s, 0.6H), 4.53 (s, 1H), 3.75-3.54 (m, 2H), 3.46-3.23 (m, 2H) 2. 86 (S, 2H) 2.62-2.50 (m, 3H), 2.35-1.96 (m, 8H)

[1769] Example 121. (S)-1-(2-(4-((3-methylquinolin-5-yl)amino)piperidin-1- yl)acetyl)pyrrolidine-2-carbonitrile [1770] (S)-1-(2-Chloroacetyl)pyrrolidine-2-carbonitrile (152.05 mg, 0.88 mmol) prepared in

Reference Example 1 was dissolved in anhydrous acetonitrile (4.4 ml). To the resulting solution, 3- methyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride (308.94 mg, 0.88 mmol) prepared in

Reference Example 35, potassium carbonate (487.0 mg, 3.52 mmol), and potassium iodide (1.46 mg, 0.01 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol= 10/1, v/v) to give the title compound (72 mg). ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.70 (s, 1H), 7.93 (s, 1H), 7.47-7.41 (m, 2H), 6.62 (d, 1H), 5.15 (d,

1H), 4.74 (s, 1H), 3.72-3.18 (m, 4H), 2.98-2.89 (m, 2H), 2.65 (s, 1H), 2.50 (s, 3H), 2.39-2.04 (m, 8H), 1.68-1.66 (m, 2H)

[1771] Example 122. (S)-1-(2-(4-((6-methylquinolin-5-yl)amino)piperidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1772] The title compound (21.8 mg) was prepared in the same fashion as Example 121, except that 6-methyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride (268.4 mg, 0.96 mmol) prepared in

Reference Example 36 was used instead of 3-methyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.82 (s, 1H), 8.38 (d, 1H), 7.70 (d, 1H), 7.49 (d, 1H), 7.35 (s, 1H), 4.74 (s, 1H), 3.72-3.52 (m, 2H), 3.34-2.93 (m, 5H), 2.44 (s, 3H), 2.27-1.63 (m, 9H), 1.26 (m, 2H)

[1773] Example 123. (S)-1-(2-(4-((7-fluoroquinolin-5-yl)amino)piperidin-1-yl)ace tyl)pyrrolidine-

2-carbonitrile

[1774] The title compound (36.4 mg) was prepared in the same fashion as Example 121, except that 7-fluoro-N-(piperidin-4-yl)quinolin-5 -amine hydrochloride (329.36 mg, 0.93 mmol) prepared in

Reference Example 37 was used instead of 3-methyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.82 (s, 1H), 8.10 (d, 1H), 7.26 (s, 1H), 7.05 (d, 1H), 6.37 (d, 1H), 4.75 (s, 1H), 4.59 (s, 1H), 3.74-3.22 (m, 5H), 3.00-2.90 (m, 2H), 2.39-1.67 (m, 8H), 1.26 (m, 2H)

[1775] Example 124. (S)-1-(2-(4-((7-methoxyquinolin-5-yl)amino)piperidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1776] The title compound (4.6 mg) was prepared in the same fashion as Example 121, except that 7-methoxy-N-(piperidin-4-yl)quinolin-5-amine hydrochloride (291.0 mg, 0.88 mmol) prepared in

Reference Example 38 was used instead of 3-methyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.76 (s, 1H), 8.04 (s, 1H), 7.19 (s, 1H), 6.87 (s, 1H), 6.28 (s, 1H), 4.77 (s, 1H), 3.75 (s, 3H), 3.65-3.15 (m, 5H), 2.99-2.90 (m, 2H), 2.40-1.46 (m, 9H), 1.26 (m, 2H)

[1777] Example 125. (S)-1-(2-(4-((8-methoxyquinolin-5-yl)amino)piperidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1778] The title compound (6.1 mg) was prepared in the same fashion as Example 121, except that 8-methoxy-N-(piperidin-4-yl)quinolin-5-amine hydrochloride (291.0 mg, 0.88 mmol) prepared in

Reference Example 39 was used instead of 3-methyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.93 (s, 1H), 8.24 (s, 1H), 7.41 (s, 1H), 6.94 (s, 1H), 6.65 (s, 1H), 4.76 (s, 1H), 4.03 (s, 3H), 3.73-3.14 (m, 7H), 3.02-2.05 (m, 9H), 1.63 (m, 2H)

[1779] Example 126. (2S,4S)-4-fluoro-1-(2-(4-((6-fluoroquinolin-4-yl)oxy)piperid in-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1780] (2S,4S)-1-(2-Chloroacetyl)-4-fluoro-pyrrolidine-2-carbonitri le (214 mg, 1.12 mmol) prepared in Reference Example 2 was dissolved in anhydrous acetonitrile (4 ml). To the resulting solution, 6-fluoro-4-(piperidin-4-yloxy)quinoline hydrochloride (400 mg, 1.12 mmol) prepared in

Reference Example 40, potassium carbonate (621.8 mg, 5.00 mmol), and potassium iodide (1.87 mg, 0.011 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol= 10/1, v/v) to give the title compound (20 mg). ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.69 (s, 1H), 8.02 (d, 1H), 7.80 (d, 1H), 7.47 (t, 1H), 6.73 (s, 1H), 5.50-5.30 (m, 1H), 4.95 (d, 1H), 4.69 (s, 1H), 4.24-3.77 (m, 2H), 3.51-3.22 (m, 2H), 2.80-2.15 (m, 10H)

[1781] Example 127. (2S,4S)-1-(2-(4-((6-chloroquinolin-4-yl)oxy)piperidin-1-yl)a cetyl)-4- fluoropyrrolidine-2-carbonitrile

[1782] The title compound (17.0 mg) was prepared in the same fashion as Example 126, except that 6-chloro-4-(piperidin-4-yloxy)quinoline hydrochloride (336.7 mg, 1.12 mmol) prepared in

Reference Example 41 was used instead of 6-fluoro-4-(piperidin-4-yloxy)quinoline hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.71 (s, 1H), 8.17 (s, 1H), 7.96 (s, 1H), 7.62 (s, 1H), 6.74 (s, 1H), 5.43 (t, 1H), 4.95 (s, 1H), 4.68 (s, 1H), 4.24-3.70 (m, 2H), 3.53-3.27 (m, 2H), 2.82-1.84 (m, 10H)

[1783] Example 128. (2S,4S)-4-fluoro-1-(2-(4-((6-methoxyquinolin-4-yl)oxy)piperi din-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1784] The title compound (24.1 mg) was prepared in the same fashion as Example 126, except that 6-methoxy-4-(piperidin-4-yloxy)quinoline hydrochloride (410.0 mg, 1.12 mmol) prepared in

Reference Example 42 was used instead of 6-fluoro-4-(piperidin-4-yloxy)quinoline hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.95 (d, 1H), 7.94 (d, 1H), 7.47 (s, 1H), 7.35 (d, 1H), 6.70 (d, 1H), 5.49-5.30 (m, 1H), 4.94 (d, 1H), 4.68 (s, 1H), 4.25-4.10 (m, 1H), 3.95 (s, 3H), 3.92-3.21 (m, 3H), 2.86-2.13 (m, 10H)

[1785] Example 129. (2S,4S)-4-fluoro-1-(2-(4-((7-methoxyquinolin-4-yl)oxy)piperi din-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1786] The title compound (4.5 mg) was prepared in the same fashion as Example 126, except that 7-methoxy-4-(piperidin-4-yloxy)quinoline hydrochloride (331.7 mg, 1.12 mmol) prepared in

Reference Example 43 was used instead of 6-fluoro-4-(piperidin-4-yloxy)quinoline hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.64 (s, 1H), 8.10 (d, 1H), 7.37 (s, 1H), 7.15 (d, 1H), 6.62 (s, 1H), 5.49-5.30 (m, 1H), 4.95 (d, 1H), 4.68 (s, 1H), 4.25-4.11 (m, 1H), 3.95 (s, 3H), 3.89-3.71 (m, 1H), 3.52-3.25 (m, 2H), 2.80-2.05 (m, 10H)

[1787] Example 130. (2S,4S)-1-(2-(4-((6,7-dimethoxyquinolin-4-yl)oxy)piperidin-1 -yl)acetyl)-4- fluoropyrrolidine-2-carbonitrile

[1788] The title compound (4.7 mg) was prepared in the same fashion as Example 126, except that 6,7-dimethoxy-4-(piperidin-4-yloxy)quinoline hydrochloride (407.1 mg, 1.12 mmol) prepared in

Reference Example 44 was used instead of 6-fluoro-4-(piperidin-4-yloxy)quinoline hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.55 (s, 1H), 7.43 (d, 1H), 7.39 (s, 1H), 6.65 (s, 1H), 5.49-5.37 (m,

1H), 4.95 (d, 1H), 4.68 (s, 1H), 4.28-4.11 (m, 1H), 4.00 (s, 6H), 3.87-3.78 (m, 1H), 3.52-3.22 (m, 2H), 2.88-2.01 (m, 10H)

[1789] Example 131. (2S,4S)-4-fluoro-1-(2-(4-((3-methylquinolin-5-yl)amino)piper idin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1790] (2S,4S)-1-(2-Chloroacetyl)-4-fluoro-pyrrolidine-2-carbonitri le (20.5 mg, 0.11 mmol) prepared in Reference Example 2 was dissolved in anhydrous acetonitrile (4 ml). To the resulting solution, 3-methyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride (37.7 mg, 0.11 mmol) prepared in Reference Example 35, potassium carbonate (59.38 mg, 0.43 mmol), and potassium iodide (0.18 mg, 0.001 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol= 10/1, v/v) to give the title compound (3 mg). ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.73 (s, 1H), 7.89 (s, 1H), 7.48-7.45 (m, 2H), 6.63

(d, 1H), 5.49-5.29 (m, 1H), 4.96 (d, 1H), 4.28-3.25 (m, 5H), 2.98-2.65 (m, 3H), 2.54 (s, 3H), 2.41- 2.05 (m, 5H), 1.88-1.26 (m, 3H)

[1791] Example 132. (2S,4S)-4-fluoro-1-(2-(4-((7-fluoroquinolin-5-yl)amino)piper idin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1792] The title compound (14 mg) was prepared in the same fashion as Example 131, except that 7-fluoro-N-(piperidin-4-yl)quinolin-5 -amine hydrochloride (30.12 mg, 0.11 mmol) prepared in

Reference Example 37 was used instead of 3-methyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) δ 8.85 (s, 1H), 8.08 (d, 1H), 7.07 (d, 1H), 6.39 (d, 1H), 5.49-5.37 (m, 1H), 4.95 (d, 1H), 4.50-3.74 (m, 3H), 3.47-3.20 (m, 3H), 3.00-2.64 (m, 3H), 2.44-2.05 (m, 6H), 1.85-1.64 (m, 2H)

[1793] Example 133. (2S,4S)-4-fluoro-1-(2-(4-((7-methoxyquinolin-5-yl)amino)pipe ridin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1794] The title compound (4 mg) was prepared in the same fashion as Example 131, except that 7-methoxy-N-(piperidin-4-yl)quinolin-5-amine hydrochloride (35.3 mg, 0.11 mmol) prepared in

Reference Example 38 was used instead of 3-methyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) δ 8.78 (s, 1H), 8.01 (d, 1H), 7.20-7.18 (m, 1H), 6.86 (s, 1H), 6.27 (s, 1H), 5.49-5.36 (m, 1H), 4.95 (d, 1H), 4.26-3.98 (m, 1H), 3.93 (s, 3H), 3.87-3.70 (m, 1H), 3.38-3.16 (m, 3H), 2.94-2.64 (m, 3H), 2.39-2.05 (m, 6H), 1.82-1.64 (m, 2H)

[1795] Example 134. (2S,4S)-4-fluoro-1-(2-(4-((6-methylquinolin-5-yl)amino)piper idin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1796] The title compound (14.1 mg) was prepared in the same fashion as Example 131, except that 6-methyl-N-(piperidin-4-yl)quinolin-5-amine(34.6 mg, 0.11 mmol) prepared in Reference Example 36 was used instead of 3-methyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H- NMR (CDC1 ₃ , 400 MHz) δ 8.83 (s, 1H), 8.38 (d, 1H), 7.71 (d, 1H), 7.50 (d, 1H), 7.37-7.35 (m, 1H),

5.48-5.35 (m, 1H), 4.93 (d, 1H), 4.22-3.72 (m, 2H), 3.42-3.15 (m, 3H), 2.96-2.63 (m, 3H), 2.44 (s, 3H), 2.36-1.26 (m, 8H)

[1797] Example 135. (2S,4S)-4-fluoro-1-(2-(4-((8-methoxyquinolin-5-yl)amino)pipe ridin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1798] The title compound (1.67 mg) was prepared in the same fashion as Example 131, except that 8-methoxy-N-(piperidin-4-yl)quinolin-5-amine hydrochloride (35.3 mg, 0.11 mmol) prepared in

Reference Example 39 was used instead of 3-methyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) δ 8.94 (s, 1H), 8.20 (d, 1H), 7.42 (d, 1H), 6.96 (d, 1H), 6.65 (d, 1H), 5.48-5.36 (m, 1H), 4.95 (d, 1H), 4.27-4.13 (m, 1H), 4.03 (s, 3H), 3.99-3.77 (m, 1H),

3.49-2.64 (m, 7H), 2.38-1.26 (m, 7H). [1799] Example 136. 6-methoxy-N-[(3S)-1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrro lidin-1- yl]ethyl]pyrrolidin-3-yl]benzofuran-3-carboxamide

[1800] The title compound (10.0 mg, 23.1 %) was prepared in the same fashion as Example 29, except that (S)-6-methoxy-N-(pyrrolidin-3-yl)benzofuran-3-carboxamide hydrochloride (37.9 mg,

0.12 mmol) in Reference Example 46 was used instead of (S)-6-fluoro-N-(pyrrolidin-3-yl)benzofuran- 3 -carboxamide hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) δ 8.24 (s, 1H), 7.89 (d, 1H), 7.13 (s, 1H), 6.97 (d, 1H), 5.51-4.98 (m, 2H), 4.60 (s, 1H), 4.07-3.99 (m, 1H), 3.85 (s, 3H), 3.82-3.39 (m, 3H), 3.07 (s, 1H), 2.87 (s, 2H), 2.61-2.36 (m, 3H), 1.87 (s, 1H)

[1801] Example 137. (2S)-1-[2-[(3R)-3-[methyl(2-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1802] The title compound (24.0 mg, 38.3 %) was prepared in the same fashion as Example 17, except that (R)-N-methyl-N-(pynOlidin-3-yl)quinolin-2-amine hydrochloride (45.5 mg, 0.17 mmol) prepared in Reference Example 47 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 7.88 (d, 1H), 7.68 (d, 1H), 7.60 (d, 1H), 7.53 (t, 1H), 7.19 (t, 1H), 6.91 (d, 1H), 5.70-5.50 (m, 1H), 5.25-4.79 (m, 1H), 3.76-3.56 (m, 2H), 3.44-3.28 (m,

2H), 3.16 (s, 3H), 3.13-2.97 (m, 2H), 2.88-2.58 (m, 2H), 2.40-2.20 (m, 3H), 2.20-2.13 (m, 2H), 1.94- 1.89 (m, 1H)

[1803] Example 138. (2S)-1-[2-[(3R)-3-[methyl(3-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1804] The title compound (109.3 mg, 47.0 %) was prepared in the same fashion as Example 17, except that (R)-N-methyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride (168.8 mg, 0.64 mmol) prepared in Reference Example 48 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.77 (s, 1H), 7.96 (d, 1H), 7.64 (d, 1H), 7.45-7.28 (m, 2H), 7.23 (s, 1H), 5.15-4.77 (m, 1H), 4.70-4.50 (m, 1H), 3.73-3.70 (m, 1H), 3.60-3.52 (m, 1H), 3.48- 3.26 (m, 2H), 3.04 (s, 3H), 3.03-2.88 (m, 1H), 2.86-2.77 (m, 2H), 2.64 (dd, 1H), 2.40-2.20 (m, 3H), 2.20-2.05 (m, 2H), 1.97-1.91 (m, 1H)

[1805] Example 139. (2S)-1-[2-[(3R)-3-[methyl(5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1806] The title compound (1.5 mg, 12.0 %) was prepared in the same fashion as Example 17, except that (R)-N-methyl-N-(pynOlidin-3-yl)quinolin-5-amine hydrochloride (88.1 mg, 0.33 mmol) prepared in Reference Example 49 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.91 (d, 1H), 8.63 (d, 1H), 7.84 (d, 1H), 7.64 (t, 1H),

7.41 (t, 1H), 7.21 (d, 1H), 5.20-4.70 (m, 1H), 4.10-3.90 (m, 1H), 3.80-3.66 (m, 1H), 3.60-3.40 (m, 2H), 3.38-3.20 (m, 2H), 3.00-2.88 (m, 1H), 2.81 (s, 3H), 2.81-2.70 (m, 2H), 2.40-2.20 (m, 2H), 2.20- 2.00 (m, 3H), 2.00-1.80 (m, 1H)

[1807] Example 140. (2S)-1-[2-[(3R)-3-[methyl(6-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1808] The title compound (16.5 mg, 19.9 %) was prepared in the same fashion as Example 17, except that (R)-N-methyl-N-(pynOlidin-3-yl)quinolin-6-amine hydrochloride (59.5 mg, 0.23 mmol) prepared in Reference Example 50 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.64 (d, 1H), 8.00-7.94 (m, 2H), 7.44 (d, 1H), 7.30-7.20 (m, 1H), 6.88 (d, 1H), 5.15-4.73 (m, 1H), 4.73-4.58 (m, 1H), 3.80-3.62 (m, 1H), 3.62-3.45 (m, 2H), 3.46-3.20 (m, 2H), 3.04 (s, 3H), 3.03-2.78 (m, 2H), 2.78-2.58 (m, 1H), 2.48-2.22 (m, 3H), 2.22-2.10 (m, 2H), 2.01-1.82 (m, 1H)

[1809] Example 141. (2S)-1-[2-[(3R)-3-[methyl(7-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1810] The title compound (74.9 mg, 51.1 %) was prepared in the same fashion as Example 17, except that (R)-N-methyl-N-(pyrrolidin-3-yl)quinolin-7-amine hydrochloride (106.3 mg, 0.40 mmol) prepared in Reference Example 51 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.75 (s, 1H), 7.97 (d, 1H), 7.65 (d, 1H), 7.28-7.23 (m, 2H), 7.12 (t, 1H), 5.20-4.74 (m, 1H), 4.74-4.61 (m, 1H), 3.82-3.64 (m, 1H), 3.64-3.50 (m, 1H), 3.46- 3.24 (m, 2H), 3.07 (s, 3H), 3.07-2.83 (m, 2H), 2.83-2.66 (m, 1H), 2.60 (q, 1H), 2.46-2.22 (m, 3H), 2.22-2.10 (m, 2H), 2.00-1.92 (m, 1H)

[1811] Example 142. (2S)-1-[2-[(3S)-3-[methyl(3-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1812] The title compound (165.8 mg, 59.1 %) was prepared in the same fashion as Example 5, except that (S)-N-methyl-N-(pynOlidin-3-yl)quinolin-3-amine hydrochloride (203.4 mg, 0.77 mmol) prepared in Reference Example 52 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.75 (s, 1H), 7.94 (d 1H), 7.63 (t, 1H), 7.44-7.41 (m, 2H), 7.22 (s, 1H), 5.16-4.70 (m, 1H), 4.70-4.52 (m, 1H), 3.80-3.60 (m, 1H), 3.60-3.48 (m, 1H), 3.48- 3.18 (m, 2H), 3.02 (s, 3H), 3.02-2.70 (m, 2H), 2.60 (dd, 1H), 2.50-2.34 (m, 1H), 2.34-2.20 (m, 3H), 2.20-2.04 (m, 2H), 2.04-1.88 (m, 1H)

[1813] Example 143. (2S)-1-[2-[(3S)-3-[methyl(4-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1814] The title compound (60.3 mg, 73.1 %) was prepared in the same fashion as Example 5, except that (S)-N-methyl-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride (59.8 mg, 0.23 mmol) prepared in Reference Example 53 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.69 (d, 1H), 8.06-8.04 (m, 2H), 7.66 (t, 1H), 7.48 (t, 1H), 6.84 (d, 1H), 5.18-4.76 (m, 1H), 4.48-4.30 (m, 1H), 3.82-3.60 (m, 1H), 3.60-3.44 (m, 1H), 3.44- 3.20 (m, 2H), 3.01 (s, 3H), 3.01-2.90 (m, 2H), 2.90-2.80 (m, 1H), 2.70-2.58 (m, 1H), 2.40-2.30 (m, 1H), 2.30-2.20 (m, 3H), 2.20-1.94 (m, 2H)

[1815] Example 144. (2S)-1-[2-[(3S)-3-[methyl(5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1816] The title compound (22.9 mg, 16.6 %) was prepared in the same fashion as Example 5, except that (S)-N-methyl-N-(pynOlidin-3-yl)quinolin-5-amine hydrochloride (100.1 mg, 0.38 mmol) prepared in Reference Example 54 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.90 (d, 1H), 8.62 (d, 1H), 7.83 (d, 1H), 7.63 (t, 1H), 7.41 (t, 1H), 7.21 (d, 1H), 5.20-4.70 (m, 1H), 4.12-3.98 (m, 1H), 3.80-3.60 (m, 1H), 3.60-3.40 (m, 1H), 3.40-3.20 (m, 2H), 3.02-2.90 (m, 1H), 2.79 (s, 3H), 2.78-2.60 (m, 3H), 2.40-2.20 (m, 2H), 2.20- 2.00 (m, 3H), 2.00-1.84 (m, 1H)

[1817] Example 145. (2S)-1-[2-[(3S)-3-[methyl(6-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1818] The title compound (85.9 mg, 64.1 %) was prepared in the same fashion as Example 5, except that (S)-N-methyl-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride (97.1 mg, 0.37 mmol) prepared in Reference Example 55 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.62 (d, 1H), 8.08-7.90 (m, 2H), 7.42 (d, 1H), 7.28-7.25 (m, 1H), 6.87 (d, 1H), 5.20-4.74 (m, 1H), 4.74-4.50 (m, 1H), 3.80-3.60 (m, 1H), 3.60-3.48 (m, 1H), 3.48-3.20 (m, 2H), 3.10 (s, 3H), 3.10-2.90 (m, 2H), 2.90-2.72 (m, 1H), 2.70-2.50 (m, 1H), 2.50-2.23 (m, 2H), 2.23-2.05 (m, 3H), 2.05-1.80 (m, 1H)

[1819] Example 146. (2S)-1-[2-[(3S)-3-[methyl(7-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1820] The title compound (142.4 mg, 49.4 %) was prepared in the same fashion as Example 5, except that (S)-N-methyl-N-(pynOlidin-3-yl)quinolin-7-amine hydrochloride (209.5 mg, 0.79 mmol) prepared in Reference Example 56 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.73 (s, 1H), 7.98 (d, 1H), 7.65 (d, 1H), 7.28-7.23 (m, 2H), 7.14-7.10 (m, 1H), 5.20-4.74 (m, 1H), 4.74-4.60 (m, 1H), 3.80-3.60 (m, 1H), 3.60-3.48 (m, 1H), 3.48-3.20 (m, 2H), 3.07 (s, 3H), 3.07-2.90 (m, 1H), 2.90-2.72 (m, 1H), 2.70-2.50 (m, 1H), 2.50-2.20 (m, 3H), 2.20-2.00 (m, 3H), 2.00-1.85 (m, 1H)

[1821] Example 147. (2S)-1-[2-[(3S)-3-[4-isoquinolyl(methyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1822] The title compound (2.4 mg, 3.3 %) was prepared in the same fashion as Example 5, except that (S)-N-methyl-N-(pyrrolidin-3-yl)isoquinolin-4-amine hydrochloride (55.6 mg, 0.21 mmol) prepared in Reference Example 57 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.98 (s, 1H), 8.24 (s, 1H), 8.19 (d, 1H), 7.97 (d, 1H), 7.70 (t, 1H), 7.61 (t, 1H), 5.25-4.74 (m, 1H), 4.23-4.10 (m, 1H), 3.80-3.60 (m, 1H), 3.60-3.40 (m,

1H), 3.40-3.20 (m, 2H), 3.05-2.90 (m, 1H), 2.86 (s, 3H), 2.86-2.78 (m, 2H), 2.78-2.66 (m, 1H), 2.40- 2.24 (m, 2H), 2.24-2.10 (m, 3H), 2.05-1.90 (m, 1H)

[1823] Example 148. (2S)-1-[2-[(3S)-3-[5-isoquinolyl(methyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1824] The title compound (10.9 mg, 11.2 %) was prepared in the same fashion as Example 5, except that (S)-N-methyl-N-(pyrrolidin-3-yl)isoquinolin-5 -amine hydrochloride (70.7 mg, 0.27 mmol) prepared in Reference Example 58 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 9.22 (s, 1H), 8.53 (d, 1H), 8.00 (d, 1H), 7.68 (d, 1H), 7.52 (t, 1H), 7.33 (d, 1H), 5.20-4.70 (m, 1H), 4.18-4.00 (m, 1H), 3.80-3.60 (m, 1H), 3.60-3.42 (m,

1H), 3.42-3.20 (m, 2H), 3.00-2.88 (m, 1H), 2.80 (s, 3H), 2.80-2.70 (m, 2H), 2.70-2.60 (m, 1H), 2.40-

2.20 (m, 2H), 2.20-2.05 (m, 3H), 2.00-1.85 (m, 1H)

[1825] Example 149. (2S)-1-[2-[(3S)-3-[methyl-(4-methyl-3-quinolyl)amino]pyrroli din-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1826] The title compound (37.4 mg, 55.9 %) was prepared in the same fashion as Example 5, except that (S)-N, 4-dimethyl -N-(pyrrolidin-3-yl)quinolin-3 -amine hydrochloride (49.1 mg, 0.18 mmol) prepared in Reference Example 59 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.79 (s, 1H), 8.05 (d, 1H), 7.99 (d, 1H), 7.64 (t, 1H), 7.55 (t, 1H), 5.20-4.70 (m, 1H), 4.10-3.96 (m, 1H), 3.78-3.60 (m, 1H), 3.60-3.44 (m, 1H), 3.40-

3.20 (m, 2H), 3.04-2.90 (m, 1H), 2.90-2.78 (m, 1H), 2.74 (s, 3H), 2.74-2.70 (m, 1H), 2.68 (s, 3H), 2.68-2.50 (m, 1H), 2.34-2.20 (m, 2H), 2.20-2.00 (m, 3H), 2.00-1.74 (m, 1H)

[1827] Example 150. (2S)-1-[2-[(3S)-3-[methyl-(6-methyl-3-quinolyl)amino]pyrroli din-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1828] The title compound (224.5 mg, 83.2 %) was prepared in the same fashion as Example 5, except that (S)-N,6-dimethyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride (198.5 mg, 0.72 mmol) prepared in Reference Example 60 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.67 (s, 1H), 7.83 (d, 1H), 7.40 (s, 1H), 7.28 (d, 1H), 7.15 (s, 1H), 5.18-4.74 (m, 1H), 4.70-4.50 (m, 1H), 3.80-3.60 (m, 1H), 3.60-3.48 (m, 1H), 3.48- 3.20 (m, 2H), 3.01 (s, 3H), 3.01-2.90 (m, 2H), 2.90-2.72 (m, 1H), 2.68-2.52 (m, 1H), 2.50 (s, 3H),

2.45-2.24 (m, 2H), 2.24-2.08 (m, 3H), 2.08-1.88 (m, 1H)

[1829] Example 151. (S)-1-(2-((S)-3-((6-fluoroquinolin-3-yl)(methyl)amino)pyrrol idin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1830] The title compound (273.7 mg, 78.8 %) was prepared in the same fashion as Example 5, except that (S)-6-fluoro-N-methyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride (256.7 mg, 0.91 mmol) prepared in Reference Example 61 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.69 (s, 1H), 7.91 (d, 1H), 7.22-7.13 (m, 2H),

7.10 (s, 1H), 5.12-4.72 (m, 1H), 4.72-4.58 (m, 1H), 3.82-3.60 (m, 1H), 3.60-3.48 (m, 1H), 3.48-3.20 (m, 2H), 3.04 (s, 3H), 3.04-2.90 (m, 2H), 2.90-2.70 (m, 1H), 2.68-2.52 (m, 1H), 2.45-2.24 (m, 2H), 2.24-2.06 (m, 3H), 2.06-1.85 (m, 1H)

[1831] Example 152. (S)-1-(2-((S)-3-((6-methoxyquinolin-3-yl)(methyl)amino)pyrro lidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1832] The title compound (196.9 mg, 67.7 %) was prepared in the same fashion as Example 5, except that (S)-6-methoxy-N-methyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride (217.2 mg, 0.74 mmol) prepared in Reference Example 62 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline- 4-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.59 (s, 1H), 7.83 (d, 1H), 7.14 (s, 1H), 7.08 (d, 1H), 6.93 (s, 1H), 5.16-4.74 (m, 1H), 4.70-4.55 (m, 1H), 3.91 (s, 3H), 3.88-3.62 (m, 1H), 3.62-3.48 (m, 1H), 3.48-3.20 (m, 2H), 3.02 (s, 3H), 3.02-2.90 (m, 2H), 2.90-2.73 (m, 1H), 2.68-2.55 (m, 1H), 2.42-2.24 (m, 2H), 2.24-2.08 (m, 3H), 2.08-1.86 (m, 1H)

[1833] Example 153. (S)-1-(2-((S)-3-((7-methoxyquinolin-3-yl)(methyl)amino)pyrro lidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1834] The title compound (61.5 mg, 31.5 %) was prepared in the same fashion as Example 5, except that (S)-7-methoxy-N-methyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride (145.5 mg, 0.50 mmol) prepared in Reference Example 63 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline- 4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.70 (s, 1H), 7.55 (d, 1H), 7.33 (s, 1H), 7.28 (s, 1H), 7.14 (d, 1H), 5.20-4.72 (m, 1H), 4.62-4.49 (m, 1H), 3.93 (s, 3H), 3.88-3.62 (m, 1H), 3.62-3.50 (m, 1H), 3.50-3.20 (m, 2H), 2.99 (s, 3H), 2.99-2.90 (m, 2H), 2.90-2.73 (m, 1H), 2.73-2.56 (m, 1H),

2.46-2.05 (m, 5H), 2.05-1.82 (m, 1H)

[1835] Example 154. (S)-1-(2-((S)-3-((8-methoxyquinolin-3-yl)(methyl)amino)pyrro lidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[1836] The title compound (180.2 mg, 45.5 %) was prepared in the same fashion as Example 5, except that (S)-8-methoxy-N-methyl-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride (295.8 mg, 1.01 mmol) prepared in Reference Example 64 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline- 4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.73 (s, 1H), 7.35 (t, 1H), 7.22-7.19 (m, 2H), 6.79 (d, 1H), 5.18-4.72 (m, 1H), 4.70-4.50 (m, 1H), 4.06 (s, 3H), 3.82-3.60 (m, 1H), 3.60-3.49 (m, 1H), 3.49-3.18 (m, 2H), 3.03 (s, 3H), 3.03-2.90 (m, 2H), 2.90-2.70 (m, 1H), 2.66-2.52 (m, 1H), 2.40- 2.22 (m, 2H), 2.22-2.08 (m, 3H), 2.05-1.82 (m, 1H)

[1837] Example 155. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[methyl-(3-methyl-5- quinolyl)amino]pyrrolidin- 1 -yl]acetyl]pyrrolidine-2-carbonitrile

[1838] The title compound (38.9 mg, 29.1 %) was prepared in the same fashion as Example 28, except that (S)-N,3-dimethyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (93.6 mg, 0.34 mmol) prepared in Reference Example 65 was used instead of (S)-N-(pyrrolidin-3 -yl)furo[3,2- c]pyridin-7 -amine hydrochloride. ¹ H-NMR (CDCI ₃ , 600MHz) δ 8.72 (s, 1H), 8.34 (s, 1H), 7.77 (d, 1H), 7.53 (t, 1H), 7.15 (d, 1H), 5.46-4.90 (m, 2H), 4.20-3.98 (m, 2H), 3.80-3.60 (m, 1H), 3.40-3.25 (m, 2H), 3.02-2.90 (m, 1H), 2.90-2.75 (m, 1H), 2.75 (s, 3H), 2.75-2.60 (m, 3H), 2.53 (s, 3H), 2.40- 2.20 (m, 1H), 2.15-2.05 (m, 1H), 1.98-1.80 (m, 1H)

[1839] Example 156. (2S,4S)-4-fluoro-1-(2-((S)-3-((3-fluoroquinolin-5- yl)(methyl)amino)pyrrolidin-1-yl)acetyl)pyrrolidine-2-carbon itrile

[1840] The title compound (264.4 mg, 79.8 %) was prepared in the same fashion as Example 28, except that (S)-3-fluoro-N-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (233.8 mg, 0.83 mmol) prepared in Reference Example 66 was used instead of (S)-N-(pyrrolidin-3 -yl)furo[3,2- c]pyridin-7 -amine hydrochloride. ¹ H-NMR (CDCI ₃ , 600MHz) δ 8.77 (s, 1H), 8.20 (d, 1H), 7.82 (d, 1H), 7.58 (t, 1H), 7.24 (d, 1H), 5.50-4.90 (m, 2H), 4.20-3.98 (m, 2H), 3.80-3.68 (m, 1H), 3.60-3.18 (m, 2H), 2.98-2.76 (m, 2H), 2.76 (s, 3H), 2.76-2.50 (m, 3H), 2.40-2.20 (m, 1H), 2.18-2.05 (m, 1H), 1.94-1.78 (m, 1H)

[1841] Example 157. (2S,4S)-4-fluoro-1-(2-((S)-3-((7-fluoroquinolin-5- yl)(methyl)amino)pyrrolidin-1-yl)acetyl)pyrrolidine-2-carbon itrile

[1842] The title compound (238.2 mg, 92.0 %) was prepared in the same fashion as Example 28, except that (S)-7-fluoro-N-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (182.5 mg, 0.65 mmol) prepared in Reference Example 67 was used instead of (S)-N-(pyrrolidin-3 -yl)furo[3,2- c]pyridin-7 -amine hydrochloride. ¹ H-NMR (CDCI ₃ , 600MHz) δ 8.85 (d, 1H), 8.47 (d, 1H), 7.39 (t, 1H), 7.33 (s, 1H), 6.92 (s, 1H), 5.48-4.90 (m, 2H), 4.20-3.88 (m, 2H), 3.80-3.68 (m, 1H), 3.60-3.18 (m, 2H), 2.98-2.80 (m, 2H), 2.76 (s, 3H), 2.76-2.60 (m, 3H), 2.40-2.20 (m, 1H), 2.18-2.08 (m, 1H), 2.00-1.88 (m, 1H)

[1843] Example 158. (2S,4S)-4-fluoro-1-(2-((S)-3-((8-fluoroquinolin-5- yl)(methyl)amino)pyrrolidin-1-yl)acetyl)pyrrolidine-2-carbon itrile

[1844] The title compound (196.6 mg, 80.9 %) was prepared in the same fashion as Example 28, except that (S)-8-fluoro-N-methyl-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (171.2 mg,

0.61 mmol) prepared in Reference Example 68 was used instead of (S)-N-(pyrrolidin-3 -yl)furo[3,2- c]pyridin-7-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 600MHz) δ 8.92 (s, 1H), 8.61 (d, 1H), 7.45 (d, 1H), 7.29 (t, 1H), 7.12 (d, 1H), 5.48-4.90 (m, 2H), 4.20-3.84 (m, 2H), 3.78-3.64 (m, 1H), 3.60-3.14 (m, 2H), 3.00-2.72 (m, 2H), 2.72 (s, 3H), 2.72-2.50 (m, 3H), 2.38-2.20 (m, 1H), 2.12-2.00 (m, 1H), 1.90-1.80 (m, 1H)

[1845] Example 159. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[methy1-[8-(trifluoromethyl)-5 - quinolyl]amino]pyrrolidin- 1 -yl]acetyl]pyrrolidine-2-carbonitrile

[1846] The title compound (324.1 mg, 68.5 %) was prepared in the same fashion as Example 28, except that (S)-N-methyl-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin -5-amine hydrochloride (349.4 mg, 1.05 mmol) prepared in Reference Example 69 was used instead of S)-N-(pyrrolidin-3 - yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 600MHz) δ 9.02 (d, 1H), 8.54 (d,

1H), 7.95 (d, 1H), 7.46 (t, 1H), 7.10 (d, 1H), 5.50-4.90 (m, 2H), 4.26-3.90 (m, 2H), 3.82-3.68 (m, 1H), 3.70-3.16 (m, 2H), 3.00-2.85 (m, 1H), 2.85 (s, 3H), 2.85-2.78 (m, 2H), 2.78-2.60 (m, 2H), 2.40-2.24 (m, 1H), 2.20-2.10 (m, 1H), 2.00-1.90 (m, 1H)

[1847] Example 160. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[methy1-[8-(trifluoromethoxy)- 5- quinolyl]amino]pyrrolidin- 1 -yl]acetyl]pyrrolidine-2-carbonitrile

[1848] The title compound (271.0 mg, 55.4 %) was prepared in the same fashion as Example 28, except that (S)-N-methyl-N-(pyrrolidin-3-yl)-8-(trifluoromethoxy)quinoli n-5-amine hydrochloride (365.6 mg, 1.05 mmol) prepared in Reference Example 70 was used instead of S)-N-(pyrrolidin-3 - yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 600MHz) δ 9.00 (d, 1H), 8.59 (d,

1H), 7.52 (d, 1H), 7.47 (t, 1H), 7.13 (d, 1H), 5.48-4.90 (m, 2H), 4.24-3.88 (m, 2H), 3.80-3.68 (m, 1H), 3.60-3.16 (m, 2H), 3.05-2.78 (m, 2H), 2.78 (s, 3H), 2.78-2.60 (m, 3H), 2.40-2.22 (m, 1H), 2.20-2.10 (m, 1H), 1.98-1.85 (m, 1H)

[1849] Example 161. (S)-1-(2-((R)-3-(quinolin-5-ylamino)pynOlidin-1-yl)acetyl)py rrolidine-2- carbonitrile

[1850] The title compound (2.5 mg, 5.19 %) was prepared in the same fashion as Example 17, except that (R)-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride prepared (25.6 mg, 0.12 mmol) in

Reference Example 71 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.87 (s, 1H), 8.31 (dd, 1H), 7.55 (t, 1H), 7.47 (d, 1H), 7.36-7.30 (m, 1H), 6.57 (d, 1H), 4.82 (dd, 1H), 4.21 (s, 1H), 3.69-3.35 (m, 4H), 3.14-2.47 (m, 4H), 2.41-1.90 (m, 7H)

[1851] Example 162. (2S)-1-[2-[(3R)-3-(6-quinolylamino)pyrrolidin-1-yl]acetyl]py rrolidine-2- carbonitrile

[1852] The title compound (11.4 mg, 22.5 %) was prepared in the same fashion as Example 17, except that (R)-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride (25.6 mg, 0.12 mmol) prepared in

Reference Example 72 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.57 (d, 1H), 7.86 (dd, 2H), 7.25-7.22 (m, 1H), 7.07 (dd, 1H), 6.62 (s,lH), 4.87-4.62 (m, 2H), 4.11 (s, 1H), 3.66-3.28 (m, 4H), 3.01-2.82 (m, 3H), 2.64-2.09 (m, 6H), 1.83-1.79 (m, 1H)

[1853] Example 163. (2S)-1-[2-[(3R)-3-(7-quinolylamino)pyrrolidin-1-yl]acetyl]py rrolidine-2- carbonitrile

[1854] The title compound (3.4 mg, 6.72 %) was prepared in the same fashion as Example 17, except that (R)-N-(pyrrolidin-3-yl)quinolin-7-amine hydrochloride (25.6 mg, 0.12 mmol) prepared in

Reference Example 73 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.71 (d, 1H), 7.94 (d, 1H), 7.57-7.54 (m,lH), 7.10 (q, 1H), 6.99 (d,lH), 6.93-6.90 (m, 1H), 4.93-4.73 (m, 2H), 4.18 (s, 1H), 3.69-3.31 (m, 4H), 3.09-2.85 (m, 3H), 2.60-2.16 (m, 6H), 1.86 (m, 1H)

[1855] Example 164. (2S)-1-[2-[(3R)-3-(8-quinolylamino)pyrrolidin-1-yl]acetyl]py rrolidine-2- carbonitrile

[1856] The title compound (9.5 mg, 18.7 %) was prepared in the same fashion as Example 17, except that (R)-N-(pyrrolidin-3-yl)quinolin-8-amine hydrochloride(25.6 mg, 0.12 mmol) prepared in Reference Example 74 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 8 δ.70-8.69 (m, 1H), 8.05 (d, 1H), 7.39-7.35 (m,2H), 7.08-7.05 (m, 1H), 6.64 (d,lH), 6.29 (d, 1H), 5.24-4.71 (m, 1H), 4.23 (s, 1H), 3.75-3.52 (m, 2.3H), 3.36-3.28 (m, 1.7H), 3.15-3.11 (m, 1H), 2.91-2.74 (m, 3H), 2.49-2.07 (m, 5H), 1.93-1.190 (m, 1H)

[1857] Example 165. (2S,4S)-4-fluoro-1-[2-[(3R)-3-(3-quinolylamino)pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1858] The title compound (15.3 mg, 39.7 %) was prepared in the same fashion as Example 28, except that (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride (25.6 mg, 0.12 mmol) prepared in

Reference Example 17 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.42-8.39 (m, 1H), 7.88 (d, 1H), 7.57 (d, 1H), 7.40-7.34 (m, 2H), 6.90-6.89 (m, 1H), 5.36-5.20 (m, 1H), 5.06-4.83 (m, 2H), 4.07-3.54 (m, 3H), 3.40-3.23 (m, 1H), 2.96-2.95 (m, 2H), 2.88-2.80 (m, 2H), 2.67-2.51 (m, 2H), 2.40-2.10 (m, 2H), 1.77-1.76 (m, 1H) [1859] Example 166. (2S,4S)-4-fluoro-1-[2-[(3R)-3-(5-quinolylamino)pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1860] The title compound (16.9 mg, 44.0 %) was prepared in the same fashion as Example 28, except that (R)-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (25.6 mg, 0.12 mmol) prepared in

Reference Example 71 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) δ 8.53 (d, 1H), 7.85 (d, 1H), 7.79 (d, 1H), 7.21-7.18 (m, 1H), 7.05-7.03 (m, 1H), 6.58-6.56 (m, 1H), 5.34-5.18 (m, 1H), 5.07-4.72 (m, 2H), 4.09-3.20 (m, 5H), 2.90-2.73 (m, 3H), 2.64-2.47 (m, 2H), 2.37-2.09 (m, 2H), 1.73-1.70 (m, 1H)

[1861] Example 167. (2S,4S)-4-fluoro-1-[2-[(3R)-3-(6-quinolylamino)pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1862] The title compound (1.2 mg, 3.1 %) was prepared in the same fashion as Example 28, except that (R)-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride (25.6 mg, 0.12 mmol) prepared in

Reference Example 72 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H NMR (CD ₃ OD, 400 MHz) δ 8.75 (dd, 1H), 8.67-8.60 (m, 1H), 7.60-7.56 (m, 1H), 7.46-7.43 (m, 1H), 7.33-7.31 (d, 1H), 6.69-6.66 (m, 1H), 5.51 (s, 1H), 5.39-5.31 (m, 1H), 5.00 (d, 1H), 4.25-4.24 (m, 1H), 4.09-4.00 (m, 1H), 3.87-3.74 (m, 1H), 3.59-3.42 (m, 1H), 3.09-3.04 (m, 2H), 2.95-2.88 (m, 1H), 2.71-2.39 (m, 4H), 1.97-1.91 (m, 1H).

[1863] Example 168. (2S,4S)-4-fluoro-1-[2-[(3R)-3-(7-quinolylamino)pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1864] The title compound (1.5 mg, 3.9 %) was prepared in the same fashion as Example 28, except that (R)-N-(pyrrolidin-3-yl)quinolin-7-amine hydrochloride (25.6 mg, 0.12 mmol) prepared in

Reference Example 73 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) δ 8.72-8.71 (m, 1H), 7.96 (d, 1H), 7.58-7.55 (m, 1H), 7.13-7.10 (m, 1H), 6.99 (s, 1H), 6.92-6.90 (m, 1H), 5.48-5.29 (m, 1H), 5.18-4.93 (m, 1H), 4.77-4.61 (m, 1H), 4.23-4.18 (m, 1H), 4.11-3.79 (m, 2H), 3.48-3.32 (m, 2H), 3.04-2.22 (m, 7H), 1.90-1.85 (m, 1H)

[1865] Example 169. (2S,4S)-4-fluoro-1-[2-[(3R)-3-(8-quinolylamino)pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1866] The title compound (16.7 mg, 43.2 %) was prepared in the same fashion as Example 28, except that (R)-N-(pyrrolidin-3-yl)quinolin-8-amine hydrochloride (25.6 mg, 0.12 mmol) prepared in

Reference Example 74 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.69-8.67 (m, 1H), 8.05-8.03 (m, 1H), 7.39-7.34 (m, 2H), 7.08-7.04 (m, 1H), 6.64-6.61 (m, 1H), 6.25 (d, 1H), 5.44-5.19 (m, 1.3H), 4.83 (d, 0.7H), 4.19- 4.17 (m, 1H), 4.05-3.65 (m, 2H), 3.39-3.07 (m, 2H), 3.00-2.02 (m, 7H), 1.92-1.85 (m, 1H)

[1867] Example 170. (2S,4S)-4-fluoro-1-[2-[(3R)-3-(4-quinolylamino)pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1868] The title compound (1.1 mg, 2.91 %) was prepared in the same fashion as Example 28, except that (R)-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride (25.6 mg, 0.12 mmol) prepared in

Reference Example 75 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H NMR (CDOD3, 400 MHz) 8 δ.38 (dd, 1H), 8.22-8.15 (m, 1H), 7.83 (d, 1H), 7.66 (t, 1H), 7.49-7.45 (m, 1H), 6.53 (d, 1H), 5.51-5.00 (m, 2H), 4.35-4.31 (m, 1H), 4.08-4.00 (m, 1H), 3.86- 3.74 (m, 1H), 3.62-3.57 (m, 1H), 3.45 (d, 1H), 3.13-2.92 (m, 3H), 2.67-2.36 (m, 4H), 1.97-1.90 (m, 1H)

[1869] Example 171. (2S,4S)-4-fluoro-1-[2-[(3R)-3-(1,8-naphthyridin-3-ylamino)py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1870] (2S)-1-(2-chloroacetyl)-4-fluoro-pyrrolidin-2-carbonitrile (168.7 mg, 0.89 mmol) prepared in Reference Example 2 was dissolved in anhydrous dichloromethane (4 ml). To the resulting solution, (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3-amine hydrochloride (254.1 mg 0.89 mmol) prepared in Reference Example 76 and potassium carbonate (492.0 mg, 3.56 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol = 10/1, v/v) to give atitle compound (50.1 mg, 15.4 %). ¹ H-NMR (CDCI ₃ , 600 MHz) δ 8.79 (s, 1H), 8.61 (d, 1H), 7.96 (d, 1H), 7.35 (t, 1H), 6.90 (d, 1H), 5.50-5.28 (m, 1H), 5.10-4.70 (m, 1H), 4.20-4.00 (m, 2H), 3.98-3.70 (m, 1H), 3.60-3.30 (m, 2H), 3.10- 2.98 (m, 2H), 2.95-2.82 (m, 2H), 2.80-2.55 (m, 2H), 2.50-2.20 (m, 2H), 1.90-1.80 (m, 1H)

[1871] Example 172. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(7-chloro-1,8-naphthyridin-3- yl)amino]pyrrolidin- 1 -yl]acetyl]pyrrolidine-2-carbonitrile

[1872] The title compound (8.5 mg, 4.2 %) was prepared in the same fashion as Example 171, except that (R)-7-chloro-N-(pynOlidin-3-yl)-1,8-naphthyridin-3-amine hydrochloride (161.2 mg, 0.50 mmol) prepared in Reference Example 77 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8- naphthyridin-3 -amine hydrochloride. ¹ H-NMR (CDCT,. 600 MH 8z).5 δ8 (s, 1H), 7.91 (d, 1H), 7.35 (d, 1H), 6.91 (s, 1H), 5.50-5.30 (m, 1H), 5.08-4.75 (m, 1H), 4.20-4.00 (m, 2H), 3.96-3.68 (m, 1H), 3.65-3.30 (m, 2H), 3.14-3.00 (m, 2H), 2.98-2.72 (m, 2H), 2.72-2.50 (m, 2H), 2.50-2.25 (m, 2H), 1.90- 1.80 (m, 1H) [1873] Example 173. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(6-fluoro-5-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1874] The title compound (138.8 mg, 63.7 %) was prepared in the same fashion as Example 171, except that (R)-6-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (171.9 mg, 0.57 mmol) prepared in Reference Example 78 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 8 δ.86 (d, 1H), 8.43 (d, 1H), 7.68 (d, 1H), 7.47- 7.39 (m, 2H), 5.52-5.35 (m, 1H), 5.35-4.94 (m, 1H), 4.30-4.06 (m, 2H), 4.04-3.70 (m, 1H), 3.70-3.38 (m, 2H), 3.18-3.00 (m, 1H), 2.98-2.85 (m, 1H), 2.82-2.60 (m, 2H), 2.60-2.40 (m, 2H), 2.40-2.20 (m, 2H), 2.00-1.85 (m, 1H)

[1875] Example 174. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(7-fluoro-5-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1876] The title compound (54.9 mg, 36.4 %) was prepared in the same fashion as Example 171, except that (R)-7-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (118.6 mg, 0.39 mmol) prepared in Reference Example 79 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 8 δ.84 (d, 1H), 8.29 (d, 1H), 7.31-7.25 (m, 1H), 7.08 (s, 1H), 6.33 (s, 1H), 5.70-5.34 (m, 1H), 5.10-4.93 (m, 1H), 4.30-3.70 (m, 3H), 3.70-3.40 (m, 2H), 3.20-3.00 (m, 2H), 2.98-2.42 (m, 4H), 2.42-2.22 (m, 2H), 2.00-1.85 (m, 1H)

[1877] Example 175. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(8-fluoro-5-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1878] The title compound (45.6 mg, 11.8 %) was prepared in the same fashion as Example 171, except that (R)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (303.3 mg, 1.00 mmol) prepared in Reference Example 80 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 8 δ.95 (d, 1H), 8.32 (d, 1H), 7.42 (t, 1H), 7.32- 7.22 (m, 1H), 6.46 (d, 1H), 5.50-5.30 (m, 1H), 5.10-4.90 (m, 1H), 4.30-3.74 (m, 3H), 3.74-3.35 (m, 2H), 3.15-2.85 (m, 2H), 2.80-2.58 (m, 4H), 2.58-2.20 (m, 2H), 2.00-1.85 (m, 1H)

[1879] Example 176. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(6-methoxy-5-quinolyl)amino]p yrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1880] The title compound (132.2 mg, 26.7 %) was prepared in the same fashion as Example 171, except that (R)-6-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (393.7 mg, 1.25 mmol) prepared in Reference Example 81 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 8 δ.76 (d, 1H), 8.39 (d, 1H), 7.79 (d, 1H), 7.46 (d, 1H), 7.33 (t, 1H), 5.50-5.24 (m, 1H), 5.24-4.90 (m, 1H), 4.25-4.00 (m, 2H), 3.96 (s, 3H), 3.96-3.70 (m, 1H), 3.70-3.30 (m, 2H), 3.10-3.00 (m, 1H), 3.00-2.82 (m, 1H), 2.80-2.32 (m, 4H), 2.32-1.95 (m, 2H), 1.90-1.78 (m, 1H)

[1881] Example 177. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(7-methoxy-5-quinolyl)amino]p yrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1882] The title compound (107.8 mg, 20.2 %) was prepared in the same fashion as Example 171, except that (R)-7-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (424.4 mg, 1.34 mmol) prepared in Reference Example 82 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) 8 δ.78 (d, 1H), 8.14 (d, 1H), 7.19 (t, 1H), 6.86 (s, 1H), 6.21 (s, 1H), 5.50-5.28 (m, 1H), 5.12-4.92 (m, 1H), 4.28-3.95 (m, 2H), 3.93 (s, 3H), 3.90-3.62 (m, 1H), 3.50-3.33 (m, 2H), 3.10-2.90 (m, 2H), 2.80-2.42 (m, 4H), 2.42-2.20 (m, 2H), 2.00-1.85 (m, 1H)

[1883] Example 178. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(8-methoxy-5-quinolyl)amino]p yrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1884] The title compound (11.1 mg, 5.3 %) was prepared in the same fashion as Example 171, except that (R)-8-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (165.7 mg, 0.52 mmol) prepared in Reference Example 83 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 8 δ.94 (d, 1H), 8.27 (d, 1H), 7.43 (t, 1H), 6.96 (d, 1H), 6.54 (d, 1H), 5.49-5.28 (m, 1H), 5.19-4.92 (m, 1H), 4.28-4.10 (m, 2H), 4.06 (s, 3H), 4.00-3.80 (m, 1H), 3.78-3.33 (m, 2H), 3.10-3.00 (m, 2H), 3.00-2.96 (m, 1H), 2.96-2.60 (m, 3H), 2.60-2.30 (m, 2H), 1.95-1.85 (m, 1H)

[1885] Example 179. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(3-methyl-5-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1886] The title compound (258.9 mg, 70.7 %) was prepared in the same fashion as Example 171, except that (R)-3-methyl-N-(pynOlidin-3-yl)quinolin-5-amine hydrochloride (288.2 mg, 0.96 mmol) prepared in Reference Example 84 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) 8 δ.72 (d, 1H), 8.06 (s, 1H), 7.50-7.42 (m, 2H), 6.54 (d, 1H), 5.48-5.28 (m, 1H), 5.15-4.98 (m, 1H), 4.30-3.62 (m, 3H), 3.60-3.32 (m, 2H), 3.12-3.00 (m, 2H), 3.00-2.90 (m, 1H), 2.80-2.60 (m, 2H), 2.53 (s, 3H), 2.53-2.40 (m, 1H), 2.40-2.18 (m, 2H), 2.00-1.85 (m, 1H)

[1887] Example 180. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(6-methyl-5-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1888] The title compound (206.3 mg, 42.3 %) was prepared in the same fashion as Example 171, except that (R)-6-methyl-N-(pynOlidin-3-yl)quinolin-5-amine hydrochloride (384.3 mg, 1.28 mmol) prepared in Reference Example 85 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.82 (d, 1H), 8.43 (d, 1H), 7.70 (d, 1H), 7.50 (d, 1H), 7.37 (t, 1H), 5.50-5.28 (m, 1H), 5.20-4.90 (m, 1H), 4.20-4.00 (m, 2H), 4.00-3.70 (m, 2H), 3.54- 3.28 (m, 2H), 3.20-3.00 (m, 1H), 2.90-2.64 (m, 2H), 2.64-2.50 (m, 2H), 2.46 (s, 3H), 2.46-2.15 (m, 2H), 1.98-1.80 (m, 1H)

[1889] Example 181. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(8-methyl-5-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1890] The title compound (106.3 mg, 31.5 %) was prepared in the same fashion as Example 171, except that (R)-8-methyl-N-(pynOlidin-3-yl)quinolin-5-amine hydrochloride (266.3 mg, 0.89 mmol) prepared in Reference Example 86 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) 8 δ.93 (d, 1H), 8.25 (d, 1H), 7.42-7.33 (m, 2H), 6.51 (d, 1H), 5.49-5.28 (m, 1H), 5.18-4.90 (m, 1H), 4.30-4.00 (m, 2H), 4.00-3.64 (m, 2H), 3.60-3.34 (m, 2H), 3.10-3.00 (m, 2H), 3.00-2.90 (m, 1H), 2.80-2.69 (m, 1H), 2.69 (s, 3H), 2.69-2.18 (m, 3H), 1.98-1.85 (m, 1H)

[1891] Example 182. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[[7-(trifluoromethyl)-5- quinolyl]amino]pyrrolidin- 1 -yl]acetyl]pyrrolidine-2-carbonitrile

[1892] The title compound (184.3 mg, 38.0 %) was prepared in the same fashion as Example 171, except that (R)-N-(pyrrolidin-3-yl)-7-(trifluoromethyl)quinolin-5-arnine hydrochloride (393.9 mg,

1.11 mmol) prepared in Reference Example 87 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8- naphthyridin-3 -amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MH 8z).9 δ5 (d, 1H), 8.40 (d, 1H), 7.74 (s, 1H), 7.44 (t, 1H), 6.64 (s, 1H), 5.78-5.40 (m, 1H), 5.03-4.97 (m, 1H), 4.38-4.20 (m, 1H), 4.10-3.74 (m, 2H), 3.70-3.35 (m, 2H), 3.22-2.85 (m, 3H), 2.82-2.42 (m, 3H), 2.42-2.22 (m, 2H), 2.05-1.88 (m, 1H)

[1893] Example 183. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[[8-(trifluoromethyl)-5- quinolyl]amino]pyrrolidin- 1 -yl]acetyl]pyrrolidine-2-carbonitrile

[1894] The title compound (116.0 mg, 54.4 %) was prepared in the same fashion as Example 171, except that (R)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-5-arnine hydrochloride (173.2 mg,

0.49 mmol) prepared in Reference Example 88 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8- naphthyridin-3 -amine hydrochloride. ¹ H-NMR (CDCI ₃ . 400 MH 9z).0 δ1 (d, 1H), 8.41 (d, 1H), 7.90 (d, 1H), 7.41 (t, 1H), 6.49 (d, 1H), 5.52-5.28 (m, 1H), 5.05-4.93 (m, 1H), 4.38-4.20 (m, 1H), 4.10-3.94 (m, 1H), 3.94-3.70 (m, 1H), 3.70-3.35 (m, 2H), 3.25-2.95 (m, 3H), 2.95-2.55 (m, 3H), 2.55-2.22 (m, 2H), 2.05-1.85 (m, 1H)

[1895] Example 184. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(3-fluoro-5-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile [1896] The title compound (255.8 mg, 87.5 %) was prepared in the same fashion as Example 171, except that (R)-3-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (230.9 mg, 0.76 mmol) prepared in Reference Example 89 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) 8 δ.77 (s, 1H), 7.99 (d, 1H), 7.52-7.49 (m, 2H), 6.61 (d, 1H), 5.54-5.28 (m, 1H), 5.10-4.95 (m, 1H), 4.30-4.14 (m, 1H), 4.10-3.94 (m, 1H), 3.94-3.65 (m, 1H), 3.65-3.37 (m, 2H), 3.18-2.88 (m, 3H), 2.85-2.58 (m, 2H), 2.55-2.20 (m, 3H), 2.00-1.95 (m, 1H)

[1897] Example 185. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(3-chloro-5-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1898] The title compound (27.4 mg, 7.4 %) was prepared in the same fashion as Example 171, except that (R)-3-chloro-N-(pynOlidin-3-yl)quinolin-5-amine hydrochloride (295.0 mg, 0.92 mmol) prepared in Reference Example 90 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 8 δ.78 (s, 1H), 8.36 (s, 1H), 7.54 (t, 1H), 7.45 (d, 1H), 6.61 (d, 1H), 5.54-5.25 (m, 1H), 5.10-4.95 (m, 1H), 4.30-4.15 (m, 1H), 4.10-3.84 (m, 1H), 3.84- 3.65 (m, 1H), 3.65-3.40 (m, 2H), 3.20-2.90 (m, 3H), 2.84-2.40 (m, 3H), 2.40-2.20 (m, 2H), 2.00-1.86 (m, 1H)

[1899] Example 186. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[[8-(trifluoromethoxy)-5- quinolyl]amino]pyrrolidin- 1 -yl]acetyl]pyrrolidine-2-carbonitrile

[1900] The title compound (200.3 mg, 37.5 %) was prepared in the same fashion as Example 171, except that (R)-N-(pyrrolidin-3-yl)-8-(trifluoromethoxy)quinolin-5-amine hydrochloride (438.0 mg, 1.18 mmol) prepared in Reference Example 91 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8- naphthyridin-3 -amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz 9).0 δ0 (d, 1H), 8.35 (d, 1H), 7.50- 7.41 (m, 2H), 6.48 (d, 1H), 5.52-5.24 (m, 1H), 5.10-4.92 (m, 1H), 4.30-4.15 (m, 1H), 4.15-3.98 (m, 1H), 3.98-3.70 (m, 1H), 3.70-3.38 (m, 2H), 3.20-2.88 (m, 3H), 2.84-2.40 (m, 3H), 2.40-2.20 (m, 2H), 2.00-1.85 (m, 1H)

[1901] Example 187. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(6-methyl-4-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1902] The title compound (3.7 mg, 6.7 %) was prepared in the same fashion as Example 171, except that (R)-6-methyl-N-(pynOlidin-3-yl)quinolin-4-amine hydrochloride (43.2 mg, 0.14 mmol) prepared in Reference Example 92 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) 8 δ.27-8.23 (m, 3H), 7.57 (d, 1H), 6.43 (d, 1H), 5.60-5.40 (m, 1H), 5.14-5.06 (m, 1H), 4.30-4.20 (m, 1H), 4.05-3.90 (m, 1H), 3.90-3.80 (m, 1H), 3.80- 3.60 (m, 2H), 3.54-3.45 (m, 1H), 3.32-3.05 (m, 3H), 3.05-2.70 (m, 2H), 2.59 (s, 3H), 2.59-2.38 (m, 1H), 2.28-2.16 (m, 1H), 2.08-1.98 (m, 1H) [1903] Example 188. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(6-methoxy-4-quinolyl)amino]p yrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1904] The title compound (37.8 mg, 10.0 %) was prepared in the same fashion as Example 171, except that (R)-6-methoxy-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride (301.1 mg, 0.95 mmol) prepared in Reference Example 93 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 8 δ.19 (d, 1H), 8.13 (d, 1H), 7.48 (s, 1H), 7.30- 7.20 (m, 1H), 6.39 (d, 1H), 5.58-5.40 (m, 1H), 5.20-5.10 (m, 1H), 4.34-4.20 (m, 1H), 4.06 (s, 3H), 4.00-3.88 (m, 1H), 3.88-3.70 (m, 1H), 3.70-3.60 (m, 2H), 3.49-3.40 (m, 1H), 3.38-3.00 (m, 3H), 3.00- 2.84 (m, 1H), 2.84-2.72 (m, 1H), 2.72-2.30 (m, 1H), 2.30-2.10 (m, 1H), 2.10-1.95 (m, 1H)

[1905] Example 189. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(8-chloro-4-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1906] The title compound (60.4 mg, 41.1 %) was prepared in the same fashion as Example 171, except that (R)-8-chloro-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride (117.0 mg, 0.37 mmol) prepared in Reference Example 94 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 8 δ.55 (d, 1H), 7.48-7.41 (m, 3H), 6.67 (d, 1H), 5.54-5.38 (m, 1H), 5.30-4.88 (m, 1H), 4.30-4.18 (m, 1H), 4.18-3.90 (m, 1H), 3.90-3.70 (m, 1H), 3.50- 3.30 (m, 2H), 3.20-3.00 (m, 1H), 3.00-2.85 (m, 1H), 2.85-2.58 (m, 3H), 2.58-2.30 (m, 2H), 2.30-2.10 (m, 1H), 2.00-1.86 (m, 1H)

[1907] Example 190. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(8-methyl-4-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1908] The title compound (140.6 mg, 27.3 %) was prepared in the same fashion as Example 171, except that (R)-8-methyl-N-(pynOlidin-3-yl)quinolin-4-amine hydrochloride (406.2 mg, 1.35 mmol) prepared in Reference Example 95 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 8 δ.61 (d, 1H), 7.74 (d, 1H), 7.50 (d, 1H), 7.35 (t, 1H), 6.41 (d, 1H), 5.50-5.30 (m, 1H), 5.10-4.90 (m, 1H), 4.34-4.18 (m, 1H), 4.18-3.82 (m, 1H), 3.82- 3.60 (m, 1H), 3.60-3.40 (m, 2H), 3.18-2.94 (m, 3H), 2.77 (s, 3H), 2.77-2.42 (m, 3H), 2.42-2.18 (m, 2H), 2.00-1.88 (m, 1H)

[1909] Example 191. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(6-fluoro-4-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1910] The title compound (65.2 mg, 15.2 %) was prepared in the same fashion as Example 171, except that (R)-6-fluoro-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride (337.7 mg, 1.11 mmol) prepared in Reference Example 96 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 8 δ.51 (s, 1H), 7.96 (d, 1H), 7.66 (d, 1H), 7.40 (d, 1H), 6.40 (d, 1H), 5.52-5.30 (m, 1H), 5.05-4.95 (m, 1H), 4.34-4.18 (m, 1H), 4.10-3.94 (m, 1H), 3.94- 3.74 (m, 1H), 3.70-3.40 (m, 2H), 3.25-3.10 (m, 1H), 3.10-2.95 (m, 2H), 2.85-2.60 (m, 2H), 2.52-2.24 (m, 3H), 2.00-1.90 (m, 1H)

[1911] Example 192. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(6-chloro-4-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1912] The title compound (109.4 mg, 31.7 %) was prepared in the same fashion as Example 171, except that (R)-6-chloro-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride (274.8 mg, 0.86 mmol) prepared in Reference Example 97 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) 8 δ.51 (d, 1H), 8.07 (s, 1H), 7.90 (d, 1H), 7.55 (d, 1H), 6.38 (d, 1H), 5.55-5.30 (m, 1H), 5.08-4.95 (m, 1H), 4.30-4.15 (m, 1H), 4.08-3.70 (m, 1H), 3.70- 3.40 (m, 2H), 3.30-3.20 (m, 1H), 3.15-3.00 (m, 2H), 2.85-2.65 (m, 2H), 2.52-2.20 (m, 3H), 2.00-1.90 (m, 1H)

[1913] Example 193. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(7-methoxy-4-quinolyl)amino]p yrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1914] The title compound (159.2 mg, 35.4 %) was prepared in the same fashion as Example 171, except that (R)-7-methoxy-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride (358.2 mg, 1.13 mmol) prepared in Reference Example 98 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 8 δ.47 (d, 1H), 7.81 (d, 1H), 7.33 (s, 1H), 7.11 (d, 1H), 6.31 (d, 1H), 5.50-5.30 (m, 1H), 5.10-4.94 (m, 1H), 4.35-4.20 (m, 1H), 4.10-3.94 (m, 1H), 3.94 (s, 3H), 3.94-3.66 (m, 2H), 3.66-3.40 (m, 2H), 3.15-2.90 (m, 2H), 2.80-2.40 (m, 2H), 2.40-2.20 (m, 3H), 2.00-1.85 (m, 1H)

[1915] Example 194. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(8-fluoro-4-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1916] The title compound (29.9 mg, 5.7 %) was prepared in the same fashion as Example 171, except that (R)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride (418.0 mg, 1.37 mmol) prepared in Reference Example 99 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (MeOD, 400 MHz) 8 δ.41 (d, 1H), 8.03 (d, 1H), 7.44-7.39 (m, 2H), 6.62 (d, 1H), 5.55-5.30 (m, 1H), 5.05-4.96 (m, 1H), 4.40-4.30 (m, 1H), 4.10-4.00 (m, 1H), 3.90-3.70 (m, 1H), 3.65-3.40 (m, 2H), 3.16-3.00 (m, 3H), 2.75-2.60 (m, 2H), 2.60-2.35 (m, 3H), 2.05-1.90 (m, 1H)

[1917] Example 195. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(8-methoxy-4-quinolyl)amino]p yrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1918] The title compound (13.2 mg, 3.3 %) was prepared in the same fashion as Example 171, except that (R)-8-methoxy-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride (316.4 mg, 1.00 mmol) prepared in Reference Example 100 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) 8 δ.57 (d, 1H), 7.67 (d, 1H), 7.41 (t, 1H), 7.05 (d, 1H), 6.44 (d, 1H), 5.55-5.30 (m, 1H), 5.20-5.00 (m, 1H), 4.35-4.20 (m, 1H), 4.07 (s, 3H), 4.07-3.90 (m, 1H), 3.90-3.70 (m, 1H), 3.70-3.45 (m, 2H), 3.25-3.15 (m, 1H), 3.15-2.90 (m, 3H), 2.84-2.50 (m, 2H), 2.50-2.25 (m, 2H), 2.06-1.90 (m, 1H)

[1919] Example 196. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[[8-(trifluoromethyl)-4- quinolyl]amino]pyrrolidin- 1 -yl]acetyl]pyrrolidine-2-carbonitrile

The title compound (30.8 mg, 5.0 %) was prepared in the same fashion as Example 171, except that (R)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-4-amine hydrochloride (499.4 mg, 1.41 mmol) prepared in Reference Example 101 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) 8 δ.64 (d, 1H), 8.22 (d, 1H), 7.97 (d, 1H), 7.44 (t, 1H), 6.43 (d, 1H), 5.52-5.28 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.15 (m, 1H), 4.04-3.84 (m, 1H), 3.80- 3.60 (m, 1H), 3.60-3.35 (m, 2H), 3.20-2.85 (m, 3H), 2.80-2.40 (m, 3H), 2.40-2.30 (m, 1H), 2.30-2.20 (m, 1H), 2.00-1.85 (m, 1H)

[1920] Example 197. (2S)-1-[2-[(3R)-3-[(7-fluoro-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1921] The title compound (207.1 mg, 90.3 %) was prepared in the same fashion as Example 17, except that (R)-7-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (189.8 mg, 0.62 mmol) prepared in Reference Example 79 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.81 (d, 1H), 8.33 (d, 1H), 7.27 (t, 1H), 7.04 (s, 1H),

6.31 (s, 1H), 5.95-5.50 (m, 1H), 4.85-4.75 (m, 1H), 4.24-4.08 (m, 1H), 3.70-3.55 (m, 2H), 3.55-3.30 (m, 2H), 3.20-2.80 (m, 3H), 2.75-2.50 (m, 1H), 2.50-2.20 (m, 4H), 2.20-2.00 (m, 2H), 2.00-1.85 (m, 1H)

[1922] Example 198. (2S)-1-[2-[(3R)-3-[(7-methoxy-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1923] The title compound (287.5 mg, 59.0 %) was prepared in the same fashion as Example 17, except that (R)-7-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (406.4 mg, 1.29 mmol) prepared in Reference Example 82 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.76 (d, 1H), 8.20 (d, 1H), 7.18 (t, 1H), 6.84 (s, 1H), 6.20 (s, 1H), 5.35-5.10 (m, 1H), 4.90-4.70 (m, 1H), 4.25-4.10 (m, 1H), 3.92 (s, 3H), 3.70-3.60 (m,

1H), 3.60-3.30 (m, 3H), 3.15-2.85 (m, 3H), 2.70-2.40 (m, 1H), 2.40-2.20 (m, 2H), 2.20-2.00 (m, 2H), 2.00-1.85 (m, 1H) [1924] Example 199. (2S)-1-[2-[(3R)-3-[(8-fluoro-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1925] The title compound (91.5 mg, 23.6 %) was prepared in the same fashion as Example 17, except that (R)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (320.6 mg, 1.05 mmol) prepared in Reference Example 80 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.93 (d, 1H), 8.36 (d, 1H), 7.42 (d, 1H), 7.29-7.24 (m, 1H), 6.44 (s, 1H), 5.18-4.90 (m, 1H), 4.90-4.70 (m, 1H), 4.30-4.10 (m, 1H), 3.70-3.60 (m, 1H), 3.60- 3.30 (m, 3H), 3.20-2.80 (m, 3H), 2.75-2.50 (m, 1H), 2.50-2.10 (m, 4H), 2.00-1.80 (m, 1H)

[1926] Example 200. (2S)-1-[2-[(3R)-3-[(8-methyl-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1927] The title compound (172.5 mg, 51.9 %) was prepared in the same fashion as Example 17, except that (R)-8-methyl-N-(pynOlidin-3-yl)quinolin-5-amine hydrochloride (275.0 mg, 0.92 mmol) prepared in Reference Example 86 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.92 (d, 1H), 8.31 (d, 1H), 7.40 (d, 1H), 7.35 (t, 1H), 6.51 (d, 1H), 4.95-4.74 (m, 1H), 4.30-4.15 (m, 1H), 3.70-3.60 (m, 1H), 3.60-3.35 (m, 3H), 3.15-2.85 (m, 3H), 2.68 (s, 3H), 2.68-2.40 (m, 2H), 2.40-2.30 (m, 1H), 2.30-2.20 (m, 1H), 2.20-2.00 (m, 2H), 2.00-1.80 (m, 1H)

[1928] Example 201. (2S)-1-[2-[(3R)-3-[[8-(trifluoromethyl)-5-quinolyl]amino]pyr rolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1929] The title compound (118.4 mg, 62.7 %) was prepared in the same fashion as Example 17, except that (R)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-5-arnine hydrochloride (160.5 mg,

0.45 mmol) prepared in Reference Example 88 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline- 3 -amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 9.00 (d, 1H), 8.48 (d, 1H), 7.88 (d, 1H), 7.42 (t, 1H), 6.47 (d, 1H), 6.30-5.70 (m, 1H), 4.85-4.75 (m, 1H), 4.30-4.15 (m, 1H), 3.70-3.60 (m, 1H), 3.60- 3.30 (m, 3H), 3.28-3.15 (m, 1H), 3.15-2.80 (m, 2H), 2.80-2.50 (m, 1H), 2.50-2.10 (m, 4H), 2.10-1.85 (m, 1H)

[1930] Example 202. (2S)-1-[2-[(3R)-3-[[8-(trifluoromethoxy)-5-quinolyl]amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1931] The title compound (97.5 mg, 17.9 %) was prepared in the same fashion as Example 17, except that (R)-N-(pyrrolidin-3-yl)-8-(trifluoromethoxy)quinolin-5-amine hydrochloride (464.2 mg, 1.25 mmol) prepared in Reference Example 91 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline- 3 -amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.98 (d, 1H), 8.42 (d, 1H), 7.49 (d, 1H), 7.43 (t, 1H), 6.45 (d, 1H), 5.70-5.30 (m, 1H), 4.85-4.75 (m, 1H), 4.30-4.15 (m, 1H), 3.70-3.56 (m, 1H), 3.56- 3.30 (m, 3H), 3.20-3.00 (m, 3H), 2.90-2.70 (m, 1H), 2.60-2.40 (m, 1H), 2.40-2.08 (m, 3H), 2.05-1.88 (m, 1H)

[1932] Example 203. (2S)-1-[2-[(3R)-3-[(3-chloro-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1933] The title compound (265.9 mg, 76.4 %) was prepared in the same fashion as Example 17, except that (R)-3-chloro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (290.8 mg, 0.91 mmol) prepared in Reference Example 90 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.75 (d, 1H), 8.43 (s, 1H), 7.53 (d, 1H), 7.43 (t, 1H), 6.59 (s, 1H), 5.60-5.20 (m, 1H), 4.85-4.75 (m, 1H), 4.30-4.15 (m, 1H), 3.70-3.60 (m, 1H), 3.60-3.30 (m, 3H), 3.20-3.10 (m, 1H), 3.10-2.95 (m, 2H), 2.80-2.50 (m, 1H), 2.50-2.20 (m, 3H), 2.20-2.00 (m, 1H), 2.00-1.85 (m, 1H)

[1934] Example 204. (2S)-1-[2-[(3R)-3-[(3-methoxy-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1935] The title compound (338.5 mg, 92.4 %) was prepared in the same fashion as Example 17, except that (R)-3-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (305.2 mg, 0.97 mmol) prepared in Reference Example 102 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.62 (d, 1H), 7.65 (s, 1H), 7.42-7.38 (m, 2H), 6.57 (d, 1H), 5.55-5.10 (m, 1H), 4.85-4.70 (m, 1H), 4.30-4.15 (m, 1H), 4.01 (s, 3H), 3.65-3.55 (m, 1H), 3.55- 3.40 (m, 3H), 3.35-3.15 (m, 1H), 3.10-2.95 (m, 2H), 2.85-2.60 (m, 1H), 2.40-2.20 (m, 2H), 2.20-2.05 (m, 2H), 2.05-1.85 (m, 1H)

[1936] Example 205. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(3-methoxy-5-quinolyl)amino]p yrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1937] The title compound (349.0 mg, 91.0 %) was prepared in the same fashion as Example 171, except that (R)-3-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (305.2 mg, 0.97 mmol) prepared in Reference Example 102 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.65 (d, 1H), 7.59 (s, 1H), 7.46-7.38 (m, 2H),

6.59 (d, 1H), 5.55-5.25 (m, 1H), 5.10-4.90 (m, 1H), 4.35-4.20 (m, 1H), 4.02 (s, 3H), 4.00-3.80 (m, 3H), 3.80-3.60 (m, 1H), 3.60-3.35 (m, 2H), 3.25-3.00 (m, 2H), 2.90-2.60 (m, 2H), 2.60-2.20 (m, 2H), 2.05-1.90 (m, 1H)

[1938] Example 206. (2S)-1-[2-[(3R)-3-[(2-chloro-6-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1939] The title compound (61.1 mg, 20.3 %) was prepared in the same fashion as Example 17, except that (R)-2-chloro-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride (251.8 mg, 0.79 mmol) prepared in Reference Example 103 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 7.86 (d, 1H), 7.78 (d, 1H), 7.27-7.23 (m, 1H), 7.09 (d, 1H), 6.64 (s, 1H), 4.90-4.75 (m, 1H), 4.20-4.10 (m, 1H), 3.75-3.60 (m, 1H), 3.60-3.30 (m, 3H), 3.10- 3.00 (m, 2H), 2.90-2.65 (m, 2H), 2.60-2.20 (m, 3H), 2.20-2.10 (m, 2H), 1.88-1.78 (m, 1H)

[1940] Example 207. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(2-chloro-6-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1941] The title compound (54.3 mg, 17.2 %) was prepared in the same fashion as Example 171, except that (R)-2-chloro-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride (251.8 mg, 0.79 mmol) prepared in Reference Example 103 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.86 (d, 1H), 7.79 (d, 1H), 7.27-7.23 (m, 1H), 7.09 (d, 1H), 6.64 (s, 1H), 5.54-5.30 (m, 1H), 5.10-4.90 (m, 1H), 4.22-4.04 (m, 1H), 4.00-3.70(m, 1H), 3.60-3.30 (m, 3H), 3.10-2.90 (m, 2H), 2.90-2.58 (m, 3H), 2.55-2.20 (m, 3H), 1.90-1.75 (m, 1H)

[1942] Example 208. (2S)-1-[2-[(3R)-3-[(3-methyl-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1943] The title compound (4.3 mg, 4.0 %) was prepared in the same fashion as Example 17, except that (R)-3-methyl-N-(pynOlidin-3-yl)quinolin-5-amine hydrochloride (89.8 mg, 0.30 mmol) prepared in Reference Example 84 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.73 (s, 1H), 8.10 (s, 1H), 7.50-7.42 (m, 2H), 6.56 (d, 1H), 5.25-4.90 (m, 1H), 4.90-4.75 (m, 1H), 4.30-4.15 (m, 1H), 3.74-3.60(m, 1H), 3.60-3.38 (m, 3H),

3.20-2.90 (m, 3H), 2.75-2.60 (m, 1H), 2.72 (s, 3H), 2.40-2.26 (m, 2H), 2.26-2.00 (m, 2H), 2.00-1.85 (m, 1H)

[1944] Example 209. (2S)-1-[2-[(3R)-3-[(3-fluoro-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1945] The title compound (73.0 mg, 66.5 %) was prepared in the same fashion as Example 17, except that (R)-3-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (120.5 mg, 0.40 mmol) prepared in Reference Example 89 was used instead of (R)-N-(pyrrolidin-3-yl)quinoline-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.77 (s, 1H), 8.04 (d, 1H), 7.52-7.46 (m, 2H), 6.62 (d, 1H), 5.30-5.05 (m, 1H), 4.88-4.78 (m, 1H), 4.30-4.15 (m, 1H), 3.74-3.60(m, 1H), 3.60-3.30 (m, 3H),

3.20-2.85 (m, 3H), 2.80-2.50 (m, 1H), 2.40-2.24 (m, 2H), 2.24-2.10 (m, 2H), 2.00-1.85 (m, 1H)

[1946] Example 210. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[[2-(trifluoromethyl)-6- quinolyl]amino]pyrrolidin- 1 -yl]acetyl]pyrrolidine-2-carbonitrile

[1947] The title compound (84.0 mg, 18.0 %) was prepared in the same fashion as Example 171, except that (R)-N-(pyrrolidin-3-yl)-2-(trifluoromethyl)quinolin-6-amine hydrochloride (379.0 mg, 1.07 mmol) prepared in Reference Example 104 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8- naphthyridin-3 -amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.03 (d, 1H), 7.96 (d, 1H), 7.58 (d, 1H), 7.16 (d, 1H), 6.66 (s, 1H), 5.52-5.30 (m, 1H), 5.10-4.90 (m, 1H), 4.30-4.10 (m, 1H), 4.10- 3.65(m, 3H), 3.65-3.34 (m, 2H), 3.12-3.00 (m, 2H), 3.00-2.80 (m, 1H), 2.80-2.58 (m, 2H), 2.54-2.20 (m, 2H), 1.98-1.80 (m, 1H)

[1948] Example 211. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(3-methyl-6-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1949] The title compound (98.8 mg, 25.9 %) was prepared in the same fashion as Example 171, except that (R)-3-methyl-N-(pynOlidin-3-yl)quinolin-6-amine hydrochloride (300.8 mg, 1.00 mmol) prepared in Reference Example 105 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.47 (s, 1H), 7.82 (d, 1H), 7.68 (s, 1H), 7.00 (d, 1H), 6.59 (s, 1H), 5.50-5.25 (m, 1H), 5.15-4.90 (m, 1H), 4.40-4.15 (m, 1H), 4.15-3.70(m, 3H), 3.54- 3.30 (m, 2H), 3.05-2.94 (m, 2H), 2.94-2.60 (m, 3H), 2.45 (s, 3H), 2.45-2.20 (m, 2H), 1.90-1.75 (m, 1H)

[1950] Example 212. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(4-methoxy-6-quinolyl)amino]p yrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1951] The title compound (44.8 mg, 16.2 %) was prepared in the same fashion as Example 171, except that (R)-4-methoxy-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride (220.7 mg, 0.70 mmol) prepared in Reference Example 106 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.50 (d, 1H), 7.82 (d, 1H), 7.08-7.03 (m, 2H), 6.66 (d, 1H), 5.50-5.30 (m, 1H), 5.20-4.90 (m, 1H), 4.30-4.18 (m, 1H), 4.18-4.05(m, 1H), 4.03 (s,

3H), 4.03-3.70 (m, 2H), 3.05-2.95 (m, 2H), 2.95-2.80 (m, 2H), 2.80-2.58 (m, 2H), 2.55-2.20 (m, 3H), 1.90-1.75 (m, 1H)

[1952] Example 213. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(8-methyl-6-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1953] The title compound (95.3 mg, 31.6 %) was prepared in the same fashion as Example 171, except that (R)-8-methyl-N-(pynOlidin-3-yl)quinolin-6-amine hydrochloride (237.2 mg, 0.79 mmol) prepared in Reference Example 107 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.65 (d, 1H), 7.88 (d, 1H), 7.29-7.24 (m, 1H), 6.96 (s, 1H), 6.52 (s, 1H), 5.50-5.28 (m, 1H), 5.20-4.90 (m, 1H), 4.25-4.15 (m, 1H), 4.15-3.70 (m, 3H), 3.55-3.30 (m, 2H), 3.05-2.95 (m, 2H), 2.95-2.75 (m, 2H), 2.72 (s, 3H), 2.72-2.55 (m, 1H), 2.50- 2.20 (m, 2H), 1.90-1.75 (m, 1H)

[1954] Example 214. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(8-fluoro-6-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1955] The title compound (220.7 mg, 59.3 %) was prepared in the same fashion as Example 171, except that (R)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride (294.2 mg, 0.97 mmol) prepared in Reference Example 110 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.63 (s, 1H), 7.90 (d, 1H), 7.32-7.27 (m, 1H),

6.82 (d, 1H), 6.45 (s, 1H), 5.50-5.30 (m, 1H), 5.14-4.90 (m, 1H), 4.20-4.00 (m, 2H), 4.00-3.65 (m, 2H), 3.60-3.30 (m, 2H), 3.10-2.90 (m, 2H), 2.90-2.70 (m, 2H), 2.70-2.50 (m, 1H), 2.50-2.20 (m, 2H), 1.90-1.70 (m, 1H)

[1956] Example 215. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[(8-chloro-6-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1957] The title compound (59.4 mg, 16.5 %) was prepared in the same fashion as Example 171, except that (R)-8-chloro-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride (287.6 mg, 0.90 mmol) prepared in Reference Example 108 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.73 (s, 1H), 7.92 (d, 1H), 7.32 (t, 1H), 7.27-7.25 (m, 1H), 6.59 (s, 1H), 5.52-5.28 (m, 1H), 5.10-4.90 (m, 1H), 4.25-4.00 (m, 2H), 4.00-3.68 (m, 2H), 3.60-3.30 (m, 2H), 3.10-3.00 (m, 2H), 2.95-2.55 (m, 3H), 2.50-2.20 (m, 2H), 1.90-1.70 (m, 1H)

[1958] Example 216. (2S,4S)-4-fluoro-1-[2-[(3R)-3-[[8-(trifluoromethyl)-6- quinolyl]amino]pyrrolidin- 1 -yl]acetyl]pyrrolidine-2-carbonitrile

[1959] The title compound (18.0 mg, 3.7 %) was prepared in the same fashion as Example 171, except that (R)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-6-amine hydrochloride (394.2 mg,

1.11 mmol) prepared in Reference Example 109 was used instead of (R)-N-(pyrrolidin-3-yl)-1,8- naphthyridin-3 -amine hydrochloride. ¹ H-NMR (CDCT,. 400MHz) δ 8.77 (d, 1H), 7.95 (d, 1H), 7.46 (s, 1H), 7.35 (t, 1H), 6.79 (s, 1H), 5.55-5.30 (m, 1H), 5.10-4.90 (m, 1H), 4.30-4.02 (m, 2H), 4.02-3.65 (m, 2H), 3.60-3.30 (m, 2H), 3.12-2.98 (m, 2H), 2.95-2.60 (m, 3H), 2.55-2.20 (m, 2H), 1.90-1.80 (m, 1H)

[1960] Example 217. (2S)-1-[2-[(3R)-3-[[2-(trifluoromethyl)-6-quinolyl]amino]pyr rolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1961] The title compound (17.6 mg, 4.6 %) was prepared in the same fashion as Example 17, except that (R)-N-(pyrrolidin-3-yl)-2-(trifluoromethyl)quinolin-6-amine hydrochloride (324.5 mg,

0.92 mmol) prepared in Reference Example 104 was used instead of (R)-N-(pyrrolidin-3-yl)quinolin- 3 -amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.04 (d, 1H), 7.96 (d, 1H), 7.58 (d, 1H), 7.17 (d, 1H), 6.67 (s, 1H), 4.90-4.75 (m, 1H), 4.25-4.15 (m, 1H), 3.75-3.60 (m, 1H), 3.60-3.45 (m, 2H), 3.45-3.30 (m, 2H), 3.10-3.00 (m, 2H), 2.95-2.55 (m, 2H), 2.50-2.10 (m, 4H), 1.95-1.80 (m, 1H) [1962] Example 218. (2S)-1-[2-[(3R)-3-[(3-methyl-6-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1963] The title compound (224.2 mg, 55.4 %) was prepared in the same fashion as Example 17, except that (R)-3-methyl-N-(pynOlidin-3-yl)quinolin-6-amine hydrochloride (334.2 mg, 1.11 mmol) prepared in Reference Example 105 was used instead of (R)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.45 (s, 1H), 7.82 (d, 1H), 7.67 (s, 1H), 7.01 (d, 1H),

6.58 (s, 1H), 4.95-4.70 (m, 1H), 4.25-4.10 (m, 1H), 3.75-3.40 (m, 3H), 3.40-3.30 (m, 2H), 3.10-2.95 (m, 2H), 2.90-2.55 (m, 2H), 2.44 (s, 3H), 2.44-2.20 (m, 2H), 2.20-1.90 (m, 2H), 1.90-1.75 (m, 1H)

[1964] Example 219. (2S)-1-[2-[(3R)-3-[(4-methoxy-6-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1965] The title compound (143.4 mg, 44.5 %) was prepared in the same fashion as Example 17, except that (R)-4-methoxy-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride (268.8 mg, 0.85 mmol) prepared in Reference Example 106 was used instead of (R)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.48 (d, 1H), 7.81 (d, 1H), 7.07-7.02 (m, 2H), 6.65 (d, 1H), 4.95-4.75 (m, 1H), 4.30-4.15 (m, 1H), 4.02 (s, 3H), 3.75-3.45 (m, 3H), 3.45-3.30 (m, 2H), 3.10- 2.95 (m, 2H), 2.90-2.50 (m, 2H), 2.50-2.30 (m, 2H), 2.30-2.00 (m, 2H), 1.90-1.75 (m, 1H)

[1966] Example 220. (2S)-1-[2-[(3R)-3-[(8-methyl-6-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1967] The title compound (200.6 mg, 97.0 %) was prepared in the same fashion as Example 17, except that (R)-8-methyl-N-(pynOlidin-3-yl)quinolin-6-amine hydrochloride (170.8 mg, 0.57 mmol) prepared in Reference Example 107 was used instead of (R)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.64 (d, 1H), 7.88 (d, 1H), 7.26-7.24 (m, 1H), 6.95 (s, 1H), 6.52 (s, 1H), 4.95-4.75 (m, 1H), 4.25-4.10 (m, 1H), 3.75-3.45 (m, 3H), 3.45-3.30 (m, 2H), 3.10- 2.95 (m, 2H), 2.90-2.80 (m, 1H), 2.72 (s, 3H), 2.70-2.50 (m, 1H), 2.50-2.30 (m, 1H), 2.30-2.10 (m, 3H), 1.90-1.75 (m, 1H)

[1968] Example 221. (2S)-1-[2-[(3R)-3-[(8-fluoro-6-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1969] The title compound (121.2 mg, 65.5 %) was prepared in the same fashion as Example 17, except that (R)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride (153.3 mg, 0.50 mmol) prepared in Reference Example 110 was used instead of (R)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.62 (s, 1H), 7.89 (d, 1H), 7.29 (t, 1H), 6.82 (d, 1H), 6.45 (s, 1H), 4.90-4.75 (m, 1H), 4.25-4.05 (m, 1H), 3.75-3.60 (m, 1H), 3.60-3.44 (m, 2H), 3.44-3.30 (m, 2H), 3.10-2.95 (m, 2H), 2.95-2.50 (m, 2H), 2.50-2.10 (m, 4H), 1.90-1.75 (m, 1H) [1970] Example 222. (2S)-1-[2-[(3R)-3-[(8-chloro-6-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1971] The title compound (77.7 mg, 57.4 %) was prepared in the same fashion as Example 17, except that (R)-8-chloro-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride (112.9 mg, 0.35 mmol) prepared in Reference Example 108 was used instead of (R)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.72 (d, 1H), 7.91 (d, 1H), 7.33-7.25 (m, 2H), 6.58 (s, 1H), 4.90-4.75 (m, 1H), 4.20-4.10 (m, 1H), 3.74-3.60 (m, 1H), 3.60-3.42 (m, 2H), 3.42-3.30 (m, 2H), 3.10-2.95 (m, 2H), 2.90-2.50 (m, 2H), 2.50-2.20 (m, 2H), 2.20-2.05 (m, 2H), 1.90-1.75 (m, 1H)

[1972] Example 223. (2S)-1-[2-[(3R)-3-[(6-methyl-4-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1973] The title compound (233.0 mg, 64.8 %) was prepared in the same fashion as Example 17, except that (R)-6-methyl-N-(pynOlidin-3-yl)quinolin-4-amine hydrochloride (297.2 mg, 0.99 mmol) prepared in Reference Example 92 was used instead of (R)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.43 (d, 1H), 7.99 (d, 1H), 7.91 (s, 1H), 7.50 (d, 1H),

6.38 (d, 1H), 4.90-4.75 (m, 1H), 4.35-4.20 (m, 1H), 3.72-3.60 (m, 2H), 3.60-3.40 (m, 3H), 3.35-3.25 (m, 1H), 3.20-2.98 (m, 2H), 2.85-2.75 (m, 1H), 2.57 (s, 3H), 2.50-2.10 (m, 4H), 2.10-1.90 (m, 1H)

[1974] Example 224. (2S)-1-[2-[(3R)-3-[(6-methoxy-4-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1975] The title compound (340.7 mg, 73.1 %) was prepared in the same fashion as Example 17, except that (R)-6-methoxy-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride (388.7 mg, 1.23 mmol) prepared in Reference Example 93 was used instead of (R)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.39 (d, 1H), 7.99 (d, 1H), 7.42 (s, 1H), 7.32 (d, 1H),

6.39 (d, 1H), 4.85-4.75 (m, 1H), 4.35-4.20 (m, 1H), 4.04 (s, 3H), 3.75-3.50 (m, 3H), 3.50-3.30 (m, 2H), 3.25-2.85 (m, 3H), 2.85-2.60 (m, 1H), 2.50-2.10 (m, 4H), 2.10-1.90 (m, 1H)

[1976] Example 225. (2S)-1-[2-[(3R)-3-[(6-fluoro-4-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1977] The title compound (267.8 mg, 64.0 %) was prepared in the same fashion as Example 17, except that (R)-6-fluoro-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride (346.5 mg, 1.14 mmol) prepared in Reference Example 96 was used instead of (R)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.50 (d, 1H), 7.95 (d, 1H), 7.72 (d, 1H), 7.38 (s, 1H),

6.39 (d, 1H), 4.90-4.75 (m, 1H), 4.35-4.15 (m, 1H), 3.75-3.60 (m, 1H), 3.60-3.35 (m, 3H), 3.25-3.15 (m, 1H), 3.15-2.90 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.35 (m, 1H), 2.35-2.10 (m, 4H), 2.00-1.85 (m, 1H) [1978] Example 226. (2S)-1-[2-[(3R)-3-[(6-chloro-4-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1979] The title compound (335.8 mg, 70.7 %) was prepared in the same fashion as Example 17, except that (R)-6-chloro-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride (397.0 mg, 1.24 mmol) prepared in Reference Example 97 was used instead of (R)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.50 (d, 1H), 8.14 (s, 1H), 7.88 (d, 1H), 7.54 (d, 1H), 6.38 (d, 1H), 4.90-4.75 (m, 1H), 4.30-4.15 (m, 1H), 3.75-3.55 (m, 2H), 3.55-3.35 (m, 3H), 3.30-3.20 (m, 1H), 3.18-2.90 (m, 2H), 2.85-2.60 (m, 1H), 2.50-2.10 (m, 4H), 2.00-1.85 (m, 1H)

[1980] Example 227. (2S)-1-[2-[(3R)-3-[(8-fluoro-4-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1981] The title compound (59.4 mg, 12.1 %) was prepared in the same fashion as Example 17, except that (R)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride (407.6 mg, 1.34 mmol) prepared in Reference Example 99 was used instead of (R)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.59 (d, 1H), 7.81 (d, 1H), 7.38-7.31 (m, 2H), 6.44 (d, 1H), 4.85-4.75 (m, 1H), 4.40-4.20 (m, 1H), 3.75-3.60 (m, 2H), 3.60-3.35 (m, 3H), 3.30-3.20 (m, 1H), 3.18-2.90 (m, 2H), 2.85-2.60 (m, 1H), 2.55-2.15 (m, 4H), 2.00-1.85 (m, 1H)

[1982] Example 228. (2S,4S)-4-fluoro-1-[2-[(3S)-3-(5-quinolylamino)pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1983] (2S,4S)-1-(2-chloroacetyl)-4-fluoro-pyrrolidin-2-carbonitril e (20.0 mg, 0.10 mmol) prepared in Reference Example 2 was dissolved in anhydrous dichloromethane (4 ml). To the resulting solution, (S)-N-(pyrrolidin-3-yl)quinoline-5-amine hydrochloride (36.0 mg 0.12 mmol) prepared in Reference Example 16 and potassium carbonate (29.0 mg, 0.21 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol = 10/1, v/v) to give atitle compound (5.3 mg, 13.7 %). ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.86 (m, 1H), 8.30 (m, 1H), 7.48-7.54 (m, 2H), 7.33 (m, 1H), 6.55 (m, 1H), 5.25-5.49 (m, 1H), 4.97 (m, 1H), 4.31 (m, 1H), 3.82-3.92 (m, 1H), 3.62-3.80 (m, 1H), 3.32-3.60 (m, 2H), 3.01-3,25 (m, 2H), 2.62-2.82 (m, 2H), 2.21-2.42 (m, 2H), 1.96-2.19 (m, 3H)

[1984] Example 229. (2S)-1-[2-[(3S)-3-(3-quinolylamino)pyrrolidin-1-yl]acetyl]py rrolidine-2- carbonitrile

[1985] The title compound (7.7 mg, 38.3 %) was prepared in the same fashion as Example 5, except that (S)-N-(pyrrolidin-3-yl)quinolin-3-amine hydrochloride (25.6 mg, 0.12 mmol) prepared in

Reference Example 6 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.44 (d, 1H), 7.93 (d, 1H), 7.60 (d, 1H), 7.41-7.39 (m, 2H), 6.96 (d, 1H), 4.87-4.67 (m, 2H), 4.12-4.11 (m, 1H), 3.65-3.62 (m, 1H), 3.55-3.41 (m, 2H), 3.09-3.00 (m, 2H), 2.86-2.79 (m, 1H), 2.67-2.05 (m, 7H), 1.88-1.79 (m, 1H).

[1986] Example 230. (2S)-1-[2-[(3S)-3-(6-quinolylamino)pyrrolidin-1-yl]acetyl]py rrolidine-2- carbonitrile

[1987] The title compound (11.4 mg, 56.5 %) was prepared in the same fashion as Example 5, except that (S)-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride (25.6mg, 0.12 mmol) prepared in

Reference Example 111 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.58 (d, 1H), 7.90 (d, 1H), 7.84 (d, 1H), 7.26-7.23 (m, 1H), 7.10-7.08 (m, 1H), 6.63 (s, 1H), 4.90-4.65 (m, 2H), 4.12-4.11 (m, 1H), 3.62-3.32 (m, 3H), 3.02-3.01 (m, 2H), 2.82-2.79 (m, 2H), 2.66-2.04 (m, 6H), 1.85-1.80 (m, 1H).

[1988] Example 231. 5-[[(3S)-1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1-yl]ethyl]pyrro lidin-3- yl]amino]quinoline-2-carbonitrile

[1989] The title compound (3.0 mg, 13.8 %) was prepared in the same fashion as Example 5, except that (S)-5-(pynOlidin-3-ylamino)quinoline-2-carbonitrile hydrochloride (37.3 mg, 0.12 mmol) prepared in Reference Example 112 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) 8 δ.62-8.42 (m, 1H), 7.66-7.58 (m, 2H), 7.49-7.43 (m, 1H), 6.67-6.63 (m, 1H), 6.06 (d, 0.6H), 5.34 (d, 0.4H), 4.79-4.71 (m, 1H), 4.25-4.20 (m, 1H), 3.70- 3.43 (m, 3H), 3.28-2.99 (m, 3H), 2.79-2.73 (m, 1H), 2.48-2.13 (m, 5H), 2.05-1.95 (m, 2H).

[1990] Example 232. 5-[[(3S)-1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1-yl]ethyl]pyrro lidin-3- yl]amino]quinoline-3-carbonitrile

[1991] The title compound (1.9 mg, 13.8 %) was prepared in the same fashion as Example 5, except that (S)-5-(pynOlidin-3-ylamino)quinoline-3-carbonitrile hydrochloride (37.3 mg, 0.12 mmol) prepared in Reference Example 113 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) δ 8.95-8.93 (m, 1.6H), 8.79 (s, 0.4H), 7.71-7.65 (m, 1H), 7.45-7.39 (m, 1H), 6.64-6.61 (m, 1H), 6.45 (d, 0.7H), 5.79 (d, 0.3H), 4.87-4.70 (m, 1H), 4.25-4.16 (m, 1H), 3.72-3.64 (m, 1H), 3.58-3.54 (m, 1.3H), 3.49-3.43 (m, 0.7H), 3.35-3.28 (m, 1H), 3.15-3.09 (m, 1H), 3.05-3.00 (m, 1H), 2.93-2.77 (m, 1H), 2.42-2.11 (m, 5H), 2.03-1.96 (m, 1H), 1.85-1.82 (m, 1H).

[1992] Example 233. (2S)-1-[2-[(3S)-3-[(3-methoxy-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1993] The title compound (10.6 mg, 24.2 %) was prepared in the same fashion as Example 5, except that (S)-3-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (37.9 mg, 0.12 mmol) prepared in Reference Example 114 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 8 δ.63 (d, 1H), 7.64-7.53 (m, 1H), 7.45-7.37 (m, 2H), 6.57 (q, 1H), 5.39-5.38 (m, 0.5H), 5.02-5.00 (m, 0.5H), 4.72 (t, 1H), 4.21 (s, 1H), 4.01 (s, 1.7H), 3.94 (s, 1.3H), 3.64-3.39 (m, 3H), 3.23-3.14 (m, 2H), 3.03-2.98 (m, 1H), 2.73-2.68 (m, 1H), 2.48-2.00 (m, 7H).

[1994] Example 234. (2S)-1-[2-[(3S)-3-[(3-fluoro-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1995] The title compound (5.7 mg, 13.3 %) was prepared in the same fashion as Example 5, except that (S)-3-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (36.5 mg, 0.12 mmol) prepared in Reference Example 115 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) 8 δ.76 (s, 1H), 7.99 (dd, 1H), 7.52-7.46 (m, 2H), 6.60 (t, 1H), 4.97-4.79 (m, 1H), 4.21-4.19 (m, 1H), 3.67-3.43 (m, 4H), 3.31-3.07 (m, 2H), 2.96 (d, 1H), 2.81- 2.71 (m, 1H), 2.48-1.94 (m, 7H).

[1996] Example 235. (2S)-1-[2-[(3S)-3-[(3-chloro-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1997] The title compound (13.7 mg, 34.4 %) was prepared in the same fashion as Example 5, except that (S)-3-chloro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (38.4 mg, 0.12 mmol) prepared in Reference Example 116 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) 8 δ.75 (s, 1H), 8.41-8.30 (m, 1H), 7.55-7.50 (m, 1H), 7.44-7.40 (m, 1H), 6.57 (d, 1H), 5.53 (d, 0.7H), 5.12 (d, 0.3H), 4.79 (d, 1H), 4.21-4.16 (m, 1H), 3.67- 3.41 (m, 3H), 3.34-3.08 (m, 2H), 2.94 (d, 1H), 2.84-2.68 (m, 1H), 2.45-1.92 (m, 7H).

[1998] Example 236. (2S)-1-[2-[(3S)-3-[(7-fluoro-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[1999] The title compound (6.8 mg, 16.1 %) was prepared in the same fashion as Example 5, except that (S)-7-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (36.5 mg, 0.12 mmol) prepared in Reference Example 117 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) 8 δ.82 (s, 1H), 8.37-8.21 (m, 1H), 7.30-7.24 (m, 1H), 7.06-7.02 (m, 1H), 6.32-6.27 (m, 1H), 5.94 (d, 0.7H), 5.40 (d, 0.3H), 4.75 (dd, 1H), 4.18-4.13 (m, 1H), 3.67-3.43 (m, 3H), 3.28-2.98 (m, 3H), 2.77-2.67 (m, 1H), 2.52-1.92 (m, 7H).

[2000] Example 237. (2S)-1-[2-[(3S)-3-[(7-methoxy-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2001] The title compound (10.1 mg, 16.1 %) was prepared in the same fashion as Example 5, except that (S)-7-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (37.9 mg, 0.12 mmol) prepared in Reference Example 118 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 8 δ.75 (s, 1H), 8.25-8.14 (m, 1H), 7.20-7.16 (m, 1H), 6.83 (s, 1H), 6.18 (d, 1H), 5.45 (d, 0.6H), 5.11 (d, 0.3H), 4.78-4.75 (m, 1H), 4.14-4.13 (m, 1H), 3.92 (s, 3H), 3.65-3.27 (m, 4H), 3.13-2.95 (m, 2H), 2.74-2.60 (m, 1H), 2.37-1.94 (m, 7H).

[2002] Example 238. (2S)-1-[2-[(3S)-3-[[7-(trifluoromethyl)-5-quinolyl]amino]pyr rolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2003] The title compound (15.0 mg, 31.0 %) was prepared in the same fashion as Example 5, except that (S)-N-(pyrrolidin-3-yl)-7-(trifluoromethyl)quinolin-5-amine hydrochloride (42.5 mg, 0.12 mmol) prepared in Reference Example 119 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 8 δ.93-8.90 (m, 1H), 8.51-8.33 (m, 1H), 7.70 (d, 1H), 7.45-7.40 (m, 1H), 6.61 (d, 1H), 6.15 (d, 0.7H), 5.51 (d, 0.3H), 4.77-4.72 (m, 1H), 4.25-4.20 (m, 1H), 3.69-3.24 (m, 4H), 3.18-3.01 (m, 2H), 2.79-2.65 (m, 1H), 2.57-1.95 (m, 7H).

[2004] Example 239. (2S)-1-[2-[(3S)-3-[(6-methyl-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2005] The title compound (3.3 mg, 7.9 %) was prepared in the same fashion as Example 5, except that (S)-6-methyl-N-(pynOlidin-3-yl)quinolin-5-amine hydrochloride (36.0 mg, 0.12 mmol) prepared in Reference Example 120 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) 8 δ.82 (s, 1H), 8.47-8.41 (m, 1H), 7.69 (d, 1H), 7.50 (d, 1H), 7.36 (q, 1H), 4.98-4.77 (m, 1H), 4.02 (s, 1H), 3.73-3.66 (m, 1H), 3.57-3.29 (m, 3H), 3.19-3.11 (m, 1H), 2.87-2.79 (m, 1H), 2.60-2.39 (m, 5H), 2.29-2.15 (m, 5H), 1.93-1.84 (m, 2H).

[2006] Example 240. (2S)-1-[2-[(3S)-3-[(6-methoxy-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2007] The title compound (5.1 mg, 11.6 %) was prepared in the same fashion as Example 5, except that (S)-6-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (37.9 mg, 0.12 mmol) prepared in Reference Example 121 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) 8 δ.77 (s, 1H), 8.43-8.38 (m, 1H), 7.77 (d, 1H), 7.47 (d, 1H), 7.34 (q, 1H), 5.13-4.76 (m, 1H), 4.11-4.05 (m, 1H), 3.98 (s, 3H), 3.75-3.65 (m, 1H), 3.58-3.35 (m, 3H), 3.04-2.98 (m, 1H), 2.81-2.78 (m, 1H), 2.70-2.36 (m, 2H), 2.32-2.05 (m, 5H), 1.97-1.82 (m, 2H).

[2008] Example 241. (2S)-1-[2-[(3S)-3-[(6-fluoro-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2009] The title compound (12.8 mg, 30.1 %) was prepared in the same fashion as Example 5, except that (S)-6-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (36.5 mg, 0.12 mmol) prepared in Reference Example 122 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 8 δ.86-8.83 (m, 1H), 8.55-8.42 (m, 1H), 7.70-7.63 (m, 1H), 7.48-7.38 (m, 2H), 5.03-4.76 (m, 1H), 4.65-4.17 (m, 2H), 3.73-3.64 (m, 1H), 3.58-3.37 (m, 3H), 3.16-3.09 (m, 1H), 2.94-2.81 (m, 1H), 2.59-2.47 (m, 1H), 2.38-2.11 (m, 6H), 2.02-1.83 (m, 1H).

[2010] Example 242. (2S)-1-[2-[(3S)-3-[(8-methyl-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2011] The title compound (15.6 mg, 37.2 %) was prepared in the same fashion as Example 5, except that (S)-8-methyl-N-(pynOlidin-3-yl)quinolin-5-amine hydrochloride (36.0 mg, 0.12 mmol) prepared in Reference Example 123 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 8 δ.91 (s, 1H), 8.34-8.26 (m, 1H), 7.40-7.33 (m, 2H), 6.51-6.47 (m, 1H), 5.03-4.74 (m, 2H), 4.22-4.14 (m, 1H), 3.69-3.28 (m, 4H), 3.10-3.05 (m, 1H),

2.94-2.92 (m, 1H), 2.76-2.63 (m, 4H), 2.47-1.91 (m, 7H)

[2012] Example 243. (2S)-1-[2-[(3S)-3-[(8-methoxy-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2013] The title compound (3.7 mg, 8.5 %) was prepared in the same fashion as Example 5, except that (S)-8-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (37.9 mg, 0.12 mmol) prepared in Reference Example 124 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) 8 δ.93 (s, 1H), 8.34-8.27 (m, 1H), 7.43-7.40 (m, 1H), 6.95 (d, 1H), 6.55-6.50 (m, 1H), 4.86-4.77 (m, 1H), 4.20-4.13 (m, 1H), 4.02 (s, 3H), 3.69-3.32 (m, 4H), 3.14-3.11 (m, 1H), 2.95-2.92 (m, 1H), 2.79-2.68 (m, 1H), 2.43-1.83 (m, 8H)

[2014] Example 244. (2S)-1-[2-[(3S)-3-[(8-fluoro-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2015] The title compound (1.9 mg, 4.3 %) was prepared in the same fashion as Example 5, except that (S)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (36.5 mg, 0.12 mmol) prepared in Reference Example 125 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) 8 δ.93 (s, 1H), 8.42-8.29 (m, 1H), 7.44-7.41 (m, 1H), 7.29-7.24 (m, 1H), 6.46-6.41 (m, 1H), 4.79-4.77 (m, 1H), 4.17 (s, 1H), 3.74-3.31 (m, 4H), 3.31-3.11 (m, 2H), 3.03-2.95 (m, 1H), 2.78-2.73 (m, 1H), 2.49-1.92 (m, 7H)

[2016] Example 245. (2S)-1-[2-[(3S)-3-[[8-(trifluoromethyl)-5-quinolyl]amino]pyr rolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2017] The title compound (9.9 mg, 20.4 %) was prepared in the same fashion as Example 5, except that (S)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-5-amine hydrochloride (42.5 mg, 0.12 mmol) prepared in Reference Example 126 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline- 4-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 8 δ.99 (d, 1H), 8.51-8.31 (m, 1H), 7.89-7.86 (m, 1H), 7.44-7.38 (m, 1H), 6.49-6.45 (m, 1H), 6.37 (d, 0.6H), 6.65 (d, 0.4H), 4.78-4.69 (m, 1H), 4.25- 4.19 (m, 1H), 3.68-3.41 (m, 3H), 3.28-2.98 (m, 3H), 2.79-2.71 (m, 1H), 2.49-1.92 (m, 7H)

[2018] Example 246. (2S)-1-[2-[(3S)-3-[[8-(trifluoromethoxy)-5-quinolyl]amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2019] The title compound (23.6 mg, 46.9 %) was prepared in the same fashion as Example 5, except that (S)-N-(pyrrolidin-3-yl)-8-(trifluoromethoxy)quinolin-5-amine hydrochloride (44.4 mg, 0.12 mmol) prepared in Reference Example 127 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline- 4-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) 8 δ.97-8.95 (m, 1H), 8.45-8.30 (m, 1H), 7.46 (d, 1H), 7.42-7.38 (m, 1H), 6.43 (t, 1H), 5.76 (d, 0.6H), 5.25 (d, 0.4H), 4.76 (s, 1H), 4.17 (s, 1H), 3.63- 3.24 (m, 3H), 3.13-2.95 (m, 3H), 2.77-2.66 (m, 1H), 2.47-1.92 (m, 7H)

[2020] Example 247. 5-[[(3S)-1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1-yl]ethyl]pyrro lidin-3- yl]amino]quinoline-8-carbonitrile

[2021] The title compound (1.2 mg, 2.8 %) was prepared in the same fashion as Example 5, except that (S)-5-(pyrrolidin-3-ylamino)quinoline-8-carbonitrile hydrochloride (37.3 mg, 0.12 mmol) prepared in Reference Example 128 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) 9 δ.02-9.00 (m, 1H), 8.62-8.34 (m, 1H), 7.92 (t, 1H), 7.14 (d, 1H), 6.51-6.48 (m, 1H), 4.80-4.67 (m, 1H), 4.26-4.22 (m, 1H), 3.75-3.43 (m, 4H), 3.31-3.14 (m, 3H), 2.85-2.80 (m, 1H), 2.44-2.01 (m, 7H)

[2022] Example 248. (2S)-1-[2-[(3S)-3-[(8-benzyloxy-5-quinolyl)amino]pyrrolidin- 1- yl]acetyl]pyrrolidine-2-carbonitrile

[2023] The title compound (19.5 mg, 29.3 %) was prepared in the same fashion as Example 5, except that (S)-8-(benzyloxy)-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (47.1 mg, 0.12 mmol) prepared in Reference Example 129 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400 MHz) 8 δ.95 (s, 1H), 8.33-8.26 (m, 1H), 7.50 (d, 2H), 7.41-7.33 (m, 3H), 7.29-7.26 (m, 1H), 6.91 (d, 1H), 6.42-6.37 (m, 1H), 5.35 (s, 2H), 4.82-4.73 (m,

1H), 4.08 (s, 1H), 3.61-3.60 (m, 1H), 3.50-3.27 (m, 3H), 3.05-3.01 (m, 1.6H), 2.89-2.86 (m, 1H), 2.72-2.61 (m, 1H), 2.42-1.86 (m, 7.4H)

[2024] Example 249. (2S,4S)-4-fluoro-1-[2-[(3S)-3-(3-quinolylamino)pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2025] The title compound (10.2 mg, 26.6 %) was prepared in the same fashion as Example 28, except that (S)-N-(pyrrolidin-3-yl)quinolin-4-amine hydrochloride (25.6 mg, 0.12 mmol) prepared in Reference Example 5 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz) δ 8.40 (d, 1H), 7.80 (d, 1H), 7.67 (d, 1H), 7.43-7.38 (m, 2H), 7.12 (s, 1H), 5.49-4.96 (m, 2H), 4.17-3.73 (m, 3H), 3.61-3.37 (m, 2H), 3.10-2.91 (m, 2H), 2.74- 2.35 (m, 5H), 1.84-1.76 (m, 1H).

[2026] Example 250. (2S,4S)-4-fluoro-1-[2-[(3S)-3-(6-quinolylamino)pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2027] The title compound (4.9 mg, 12.8 %) was prepared in the same fashion as Example 28, except that (S)-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride (25.6 mg, 0.12 mmol) prepared in Reference Example 111 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz) δ 8.60 (d, 1H), 7.91-7.84 (m, 2H), 7.27-7.25 (m, 1H), 7.09 (t, 1H), 6.63 (s, 1H), 5.46-5.13 (m, 1H), 5.15=4.90 (m, 1H), 4.59-4.47 (m, 1H), 4.17-3.66 (m, 3H), 3.52-3.32 (m, 2H), 3.10-2.99 (m, 1H), 2.88-2.61 (m, 3H), 2.48-2.21 (m, 3H), 1.88-1.81 (m, 1H).

[2028] Example 251. (2S,4S)-4-fluoro-1-[2-[(3S)-3-(7-quinolylamino)pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2029] The title compound (12.2 mg, 31.6 %) was prepared in the same fashion as Example 28, except that (S)-N-(pyrrolidin-3-yl)quinolin-7-amine hydrochloride (25.6 mg, 0.12 mmol) prepared in Reference Example 130 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz) δ 8.56 (s, 1H), 8.07 (d, 1H), 7.62 (d, 1H), 7.16-7.13 (m, 1H), 7.05 (d, 1H), 6.84 (s, 1H), 5.49-4.95 (m, 2H), 4.19-3.71 (m, 3H), 3.59-3.37 (m, 2H), 3.05-2.95 (m, 2H), 2.81-2.35 (m, 5H), 1.87-1.78 (m, 1H).

[2030] Example 252. 5-[[(3S)-1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrrolidin-1- yl]ethyl]pyrrolidin-3-yl]amino]quinoline-2-carbonitrile

[2031] The title compound (3.8 mg, 9.2 %) was prepared in the same fashion as Example 28, except that (S)-5-(pyrrolidin-3-ylamino)quinoline-2-carbonitrile hydrochloride (37.3 mg, 0.12 mmol) prepared in Reference Example 112 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz) δ 8.77-8.71 (m, 1H), 7.72-7.64 (m, 2H), 7.34 (d, 1H), 6.73 (d, 1H), 5.49-4.99 (m, 2H), 4.20-3.73 (m, 3H), 3.66-3.40 (m, 2H), 3.05-2.87 (m, 3H), 2.70- 2.39 (m, 4H), 1.94-1.86 (m, 1H).

[2032] Example 253. 5-[[(3S)-1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrrolidin-1- yl] ethyl]py rrolidin-3 -yl] amino] quinoline-3 -carbonitrile

[2033] The title compound (4.4 mg, 10.7 %) was prepared in the same fashion as Example 28, except that (S)-5-(pyrrolidin-3-ylamino)quinoline-3-carbonitrile hydrochloride (37.3 mg, 0.12 mmol) prepared in Reference Example 113 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz) 9 δ.12 (d, 1H), 8.92 (s, 1H), 7.73 (t, 1H), 7.33 (d, 1H), 6.74 (d, 1H), 5.52-4.99 (m, 2H), 4.30-3.74 (m, 3H), 3.66-3.42 (m, 2H), 3.11-2.89 (m, 3H), 2.70- 2.37 (m, 4H), 1.97-1.89 (m, 1H).

[2034] Example 254. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(3-methoxy-5-quinolyl)amino]p yrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2035] The title compound (2.3 mg, 5.4 %) was prepared in the same fashion as Example 28, except that (S)-3-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (37.9 mg, 0.12 mmol) prepared in Reference Example 114 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz) 8 δ.51 (s, 1H), 7.97 (t, 1H), 7.43-7.28 (m, 2H), 6.76-6.68 (m, 1H), 5.50-4.99 (m, 2H), 4.30-3.92 (m, 5H), 3.82-3.42 (m, 4H), 3.14-2.91 (m, 2H), 2.70- 2.31 (m, 4H), 2.23-1.92 (m, 1H).

[2036] Example 255. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(3-fluoro-5-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2037] The title compound (4.7 mg, 11.7 %) was prepared in the same fashion as Example 28, except that (S)-3-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (36.5 mg, 0.12 mmol) prepared in Reference Example 115 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz) 8 δ.70 (d, 1H), 8.39-8.32 (m, 1H), 7.51 (t, 1H), 7.34 (d, 1H), 6.64 (d, 1H), 5.49-4.97 (m, 2H), 4.23-4.15 (m, 1H), 4.05-3.38 (m, 4H), 3.07-2.85 (m, 3H), 2.67-2.35 (m, 4H), 1.92-1.85 (m, 1H).

[2038] Example 256. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(3-chloro-5-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2039] The title compound (4.9 mg, 11.8 %) was prepared in the same fashion as Example 28, except that (S)-3-chloro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (38.4 mg, 0.12 mmol) prepared in Reference Example 116 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz) 8 δ.69-8.67 (m, 2H), 7.55 (t, 1H), 7.29 (d, 1H), 6.64 (d, 1H), 5.49-4.97 (m, 2H), 4.23-4.15 (m, 1H), 4.05-3.71 (m, 2H), 3.63-3.38 (m, 2H), 3.08-2.89 (m, 3H), 2.66-2.36 (m, 4H), 1.90-1.87 (m, 1H).

[2040] Example 257. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(7-methoxy-5-quinolyl)amino]p yrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2041] The title compound (3.0 mg, 7.2 %) was prepared in the same fashion as Example 28, except that (S)-7-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (37.9 mg, 0.12 mmol) prepared in Reference Example 118 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz) 8 δ.60 (d, 1H), 8.48-8.42 (m, 1H), 7.24 (q, 1H), 6.70 (s, 1H), 6.19 (s, 1H), 5.49-4.95 (m, 2H), 4.13-4.09 (m, 1H), 4.00-3.91 (dd, 1H), 3.89 (s, 3H), 3.81-3.66 (m, 1H), 3.61-3.36 (m, 2H), 3.04-2.88 (m, 3H), 2.60-2.32 (m, 4H), 1.88-1.85 (m, 1H).

[2042] Example 258. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[[7-(trifluoromethyl)-5- quinolyl]amino]pyrrolidin- 1 -yl]acetyl]pyrrolidine-2-carbonitrile

[2043] The title compound (3.5 mg, 7.7 %) was prepared in the same fashion as Example 28, except that (S)-N-(pyrrolidin-3-yl)-7-(trifluoromethyl)quinolin-5-amine hydrochloride (42.5 mg, 0.12 mmol) prepared in Reference Example 119 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2- c]pyridin-7-amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz) δ 8.87 (d, 1H), 8.74-8.67 (m, 1H), 7.58-7.55 (m, 2H), 6.68 (s, 1H), 5.51-4.99 (m, 2H), 4.29-4.24 (m, 1H), 4.07-3.98 (dd, 1H), 3.85- 3.73(m, 1H), 3.68-3.42 (m, 2H), 3.12-2.92 (m, 3H), 2.71-2.37 (m, 4H), 1.97-1.90 (m, 1H).

[2044] Example 259. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(6-methyl-5-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2045] The title compound (5.3 mg, 13.3 %) was prepared in the same fashion as Example 28, except that (S)-6-methyl-N-(pynOlidin-3-yl)quinolin-5-amine hydrochloride (36.0 mg, 0.12 mmol) prepared in Reference Example 120 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz) δ 8.71 (d, 1H), 8.67-8.61 (m, 1H), 7.57 (q, 2H), 7.48 (q, 1H), 5.49-4.96 (m, 2H), 4.08-3.99 (m, 2H), 3.83-3.71 (m, 1H), 3.59-3.42(m, 1H), 3.32-3.28 (m, 1H), 3.12-3.05 (m, 1H), 2.86-2.80 (m, 1H), 2.70-2.32 (m, 7H), 2.25-2.16 (m, 1H), 1.92-1.81 (m, 1H).

[2046] Example 260. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(6-methoxy-5-quinolyl)amino]p yrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2047] The title compound (2.5 mg, 5.9 %) was prepared in the same fashion as Example 28, except that (S)-6-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (37.9 mg, 0.12 mmol) prepared in Reference Example 121 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz) δ 8.67 (d, 1H), 8.58-8.53 (m, 1H), 7.70 (d, 1H), 7.65-7.62 (m, 1H), 7.45 (dd, 1H), 5.49-4.96 (m, 2H), 4.12-4.00 (m, 5H), 3.87-3.75 (m, 1H), 3.62- 3.32(m, 2H), 3.04-2.99 (m, 1H), 2.85-2.36 (m, 5H), 2.23-2.09 (m, 1H), 1.95-1.76 (m, 1H).

[2048] Example 261. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(6-fluoro-5-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2049] The title compound (4.6 mg, 11.3 %) was prepared in the same fashion as Example 28, except that (S)-6-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (36.5 mg, 0.12 mmol) prepared in Reference Example 122 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz) δ 8.75-8.64 (m, 2H), 7.54-7.50 (m, 3H), 5.49-4.98 (m, 2H), 4.35 (br s, 1H), 4.06-3.72 (m, 2H), 3.61-3.32 (m, 2H), 3.12-3.06 (m, 1H), 2.95-2.68 (m,

2H), 2.64-2.19 (m, 4H), 1.94-1.82 (m, 1H).

[2050] Example 262. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(8-methyl-5-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2051] The title compound (8.6 mg, 21.6 %) was prepared in the same fashion as Example 28, except that (S)-8-methyl-N-(pynOlidin-3-yl)quinolin-5-amine hydrochloride (36.0 mg, 0.12 mmol) prepared in Reference Example 123 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz) 8 δ.78 (s, 1H), 8.60-8.58 (m, 1H), 7.41 (br s, 2H), 6.56 (d, 1H), 5.49-4.96 (m, 2H), 4.17 (br s, 1H), 4.06-3.71 (m, 2H), 3.58-3.38 (m, 2H), 3.00-2.88

(m, 3H), 2.64-2.39 (m, 7H), 1.94-1.84 (m, 1H).

[2052] Example 263. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(8-methoxy-5-quinolyl)amino]p yrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2053] The title compound (11.5 mg, 27.6 %) was prepared in the same fashion as Example 28, except that (S)-8-methoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (37.9 mg, 0.12 mmol) prepared in Reference Example 124 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400 MHz 8).9 δ3 (s, 1H), 8.29 (d, 1H), 7.43-7.40 (m, 1H), 6.95 (d, 1H), 6.52 (dd, 1H), 5.49-4.95 (m, 2H), 4.19-3.67 (m, 6H), 3.55-3.36 (m, 2H), 3.17-2.89 (m, 2H), 2.77-2.61 (m, 2H), 2.52-2.13 (m, 3H), 2.05-1.86 (m, 2H).

[2054] Example 264. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(8-fluoro-5-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2055] The title compound (1.8 mg, 4.5 %) was prepared in the same fashion as Example 28, except that (S)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (36.5 mg, 0.12 mmol) prepared in Reference Example 125 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz) 8 δ.82 (d, 1H), 8.69-8.63 (m, 1H), 7.54 (dd, 1H), 7.33 (t, 1H), 6.57 (dd, 1H), 5.50-5.38 (m, 1H), 5.32-4.98 (m, 1H), 4.24-4.17 (m, 1H), 4.14-3.99 (m, 1H), 3.86-3.73 (m, 1H), 3.67-3.44 (m, 2H), 3.09-2.88 (m, 3H), 2.75-2.33 (m, 4H), 1.93-1.88 (m, 1H).

[2056] Example 265. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[[8-(trifluoromethyl)-5- quinolyl]amino]pyrrolidin- 1 -yl]acetyl]pyrrolidine-2-carbonitrile

[2057] The title compound (3.9 mg, 8.6 %) was prepared in the same fashion as Example 28, except that (S)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-5-amine hydrochloride (42.5 mg, 0.12 mmol) prepared in Reference Example 126 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2- c]pyridin-7-amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz 8).8 δ5 (d, 1H), 8.69-8.62 (m, 1H), 7.88 (d, 1H), 7.49 (dd, 1H), 6.58 (d, 1H), 5.49-4.98 (m, 2H), 4.24 (br s, 1H), 4.06-3.97 (m, 1H), 3.83- 3.40 (m, 3H), 3.09-2.90 (m, 3H), 2.68-2.36 (m, 4H), 1.98-1.88 (m, 1H).

[2058] Example 266. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[[8-(trifluoromethoxy)-5- quinolyl]amino]pyrrolidin- 1 -yl]acetyl]pyrrolidine-2-carbonitrile

[2059] The title compound (5.5 mg, 11.7 %) was prepared in the same fashion as Example 28, except that (S)-N-(pyrrolidin-3-yl)-8-(trifluoromethoxy)quinolin-5-amine hydrochloride (44.4 mg, 0.12 mmol) prepared in Reference Example 127 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2- c]pyridin-7-amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz 8). δ84 (d, 1H), 8.69-8.63 (m, 1H), 7.53-7.50 (m, 2H), 6.56 (d, 1H), 5.49-5.37 (m, 1H), 5.30-4.98 (m, 1H), 4.19 (br s, 1H), 4.06-3.97 (m, 1H), 3.83-3.72 (m, 1H), 3.66-3.40 (m, 2H), 3.08-2.88 (m, 3H), 2.69-2.36 (m, 4H), 1.91-1.88 (m, 1H).

[2060] Example 267. 5-[[(3S)-1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrrolidin-1- yl] ethyl]py rrolidin-3 -yl] amino] quinoline-8 -carbonitrile

[2061] The title compound (2.6 mg, 6.3 %) was prepared in the same fashion as Example 28, except that (S)-5-(pyrrolidin-3-ylamino)quinoline-8-carbonitrile hydrochloride (37.3 mg, 0.12 mmol) prepared in Reference Example 128 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz) 8 δ.89 (d, 1H), 8.77-8.69 (m, 1H), 7.98 (d, 1H), 7.53 (dd, 1H), 6.68 (d, 1H), 5.50-5.38 (m, 1H), 5.31-5.00 (m, 1H), 4,38-4.30 (m, 1H), 4.09-4.00 (m, 1H), 3.86-3.43 (m, 3H), 3.13-2.92 (m, 3H), 2.72-2.41 (m, 4H), 2.02-1.96 (m, 1H).

[2062] Example 268. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(8-benzyloxy-5-quinolyl)amino ]pyrrolidin- l-yl]acetyl]pyrrolidine-2 -carbonitrile

[2063] The title compound (3.2 mg, 6.4 %) was prepared in the same fashion as Example 28, except that (S)-8-(benzyloxy)-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (47.1 mg, 0.12 mmol) prepared in Reference Example 129 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2- c]pyridin-7-amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz 8).7 δ8 (d, 1H), 8.58-8.53 (m,

1H), 7.51-7.45 (m, 3H), 7.35-7.25 (m, 3H), 7.03 (d, 1H), 6.47 (d, 1H), 5.49-5.25 (m, 3.2H), 4.95-

4.93 (m, 0.8H), 4.04-3.64 (m, 3H), 3.58-3.29 (m, 2H), 2.97-2.80 (m, 3H), 2.58-2.28 (m, 4H), 1.87- 1.80 (m, 1H).

[2064] Example 269. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(7-fluoro-5-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2065] The title compound (7.2 mg, 15.9 %) was prepared in the same fashion as Example 28, except that (S)-7-fluoro-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (36.5 mg, 0.12 mmol) prepared in Reference Example 117 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) 8 δ.79 (t, 1H), 8.30-8.22 (m, 1H), 7.26-7.23 (m, 1H), 7.04-7.00 (m, 1H), 6.28 (m, 1H), 5.71-5.46 (m, 1H), 5.38-5.25 (m, 1H), 4.89 (t, 1H), 4.15-3.44 (m, 3H), 3.34-3.26 (m, 1H), 3.17-2.94 (m, 2H), 2.73-2.22 (m, 6H), 2.03-1.91 (m, 1H).

[2066] Example 270. (2S)-1-[2-[(3S)-3-[[8-(trifluoromethyl)-4-quinolyl]amino]pyr rolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2067] The title compound (12.2 mg, 24.3 %) was prepared in the same fashion as Example 5, except that (S)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-4-amine hydrochloride (42.5 mg, 0.12 mmol) prepared in Reference Example 131 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4- amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.68 (t, 1H), 8.18 (dd, 1H), 8.00 (t, 1H), 7.47 (t, 1H), 6.45 (t, 1H), 6.21 (dd, 1H), 5.52-5.30 (m, 1H), 4.93 (dd, 1H), 4.26-3.54 (m, 4H), 3.45-3.02 (m, 3.5H), 2.78-2.26 (m, 4.5H), 2.10-1.88 (m, 1H).

[2068] Example 271. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[[8-(trifluoromethyl)-4- quinolyl]amino]pyrrolidin- 1 -yl]acetyl]pyrrolidine-2-carbonitrile

[2069] The title compound (8.0 mg, 15.4 %) was prepared in the same fashion as Example 28, except that (S)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-4-amine hydrochloride (42.5 mg, 0.12 mmol) prepared in Reference Example 131 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2- c]pyridin-7-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.68 (t, 1H), 8.22 (dd, 1H), 8.00 (t, 1H), 7.47 (dd, 1H), 6.47 (t, 1H), 6.42 (dd, 1H), 4.82-4.69 (m, 1H), 4.24 (d, 1H), 3.66-3.45 (m, 3H), 3.30-3.01 (m, 3H), 2.82-2.76 (m, 1H), 2.50-2.13 (m, 5H), 2.06-1.99 (m, 1H).

[2070] Example 272. (2S,4S)-4-fluoro-1-[2-[(3S)-3-(1,8-naphthyridin-3-ylamino)py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2071] The title compound (67.7 mg, 26.6 %) was prepared in the same fashion as Example 28, except that (S)-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3-amine hydrochloride (196.7 mg, 0.69 mmol) prepared in Reference Example 132 was used instead of (S)-N-(pyrrolidin-3 -yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 600MHz) δ 8.79 (d, 1H), 8.62 (s, 1H), 7.96 (d, 1H), 7.35 (t, 1H), 6.90 (s, 1H), 5.50-5.30 (m, 1H), 5.10-4.70 (m, 1H), 4.20-4.05 (m, 1H), 3.95-3.70 (m, 2H), 3.60- 3.35 (m, 2H), 3.15-3.00 (m, 2H), 2.90-2.65 (m, 3H), 2.50-2.25 (m, 3H), 1.95-1.80 (m, 1H)

[2072] Example 273. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(7-chloro-1,8-naphthyridin-3- yl)amino]pyrrolidin- 1 -yl]acetyl]pyrrolidine-2-carbonitrile

[2073] The title compound (10.1 mg, 5.2 %) was prepared in the same fashion as Example 28, except that (S)-7-chloro-N-(pyrrolidin-3-yl)-1,8-naphthyridin-3-amine hydrochloride (155.6 mg, 0.48 mmol) prepared in Reference Example 133 was used instead of (S)-N-(pyrrolidin-3 -yl)furo[3,2- c]pyridin-7-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 600MHz) δ 8.58 (s, 1H), 7.90 (d, 1H), 7.34 (d, 1H), 6.90 (s, 1H), 5.50-5.30 (m, 1H), 5.10-4.90 (m, 1H), 4.20-4.00 (m, 2H), 4.00-3.65 (m, 1H), 3.60- 3.30 (m, 2H), 3.20-3.00 (m, 2H), 2.90-2.60 (m, 3H), 2.50-2.25 (m, 3H), 1.95-1.80 (m, 1H) [2074] Example 274. (2S)-1-[2-[(3S)-3-[(8-ethoxy-5-quinolyl)amino]pyrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2075] The title compound (3.4 mg, 7.5 %) was prepared in the same fashion as Example 5, except that (S)-8-ethoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (39.6 mg, 0.12 mmol) prepared in Reference Example 134 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4-amine hydrochloride. ¹ H NMR (CDC1 ₃ , 400 MHz) 8 δ.93-8.91 (m, 1H), 8.31-8.24 (m, 1H), 7.39-7.35 (m, 1H), 6.94 (d, 1H), 6.49 (dd, 1H), 4.85-4.72 (m, 1H), 4.66-4.45 (m, 1H), 4.23 (q, 2H), 4.13-4.09 (m, 1H), 3.65-3.28 (m, 4H), 3.09-2.99 (m, 1H), 2.89-2.86 (m, 1H), 2.75-2.63 (m, 1H), 2.45-1.83 (m, 7H), 1.56 (t, 3H).

[2076] Example 275. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(8-ethoxy-5-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2077] The title compound (3.5 mg, 8.0 %) was prepared in the same fashion as Example 28, except that (S)-8-ethoxy-N-(pyrrolidin-3-yl)quinolin-5-amine hydrochloride (39.6 mg, 0.12 mmol) prepared in Reference Example 134 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) 8 δ.90 (t, 1H), 8.26 (d, 1H), 7.37-7.33 (m, 1H), 6.93 (d, 1H), 6.46 (dd, 1H), 5.41-5.17 (m, 1H), 5.07-4.84 (m, 1H), 4.54-4.39 (m, 1H), 4.20 (q, 2H), 4.13-3.99 (m, 1.5H), 3.89-3.58 (m, 1.5H), 3.50-3.25 (m, 2H), 3.09-2.81 (m, 2H), 2.72-2.08 (m, 5H), 1.98-1.80 (m, 1H), 1.54 (td, 3H).

[2078] Example 276. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(8-hydroxy-5-quinolyl)amino]p yrrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2079] The title compound (2.5 mg, 5.4 %) was prepared in the same fashion as Example 28, except that (S)-5-(pynOlidin-3-ylamino)quinolin-8-ol hydrochloride (36.2 mg, 0.12 mmol) prepared in Reference Example 135 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.78 (s, 1H), 8.31 (d, 1H), 7.69 (br s, 1H), 7.42 (dd, 1H), 7.06 (d, 1H), 6.53 (dd, 1H), 5.50-5.24 (m, 1H), 5.07-4.95 (m, 1H), 4.19-4.11 (m, 2H), 3.96-3.69 (m, 2H), 3.56-3.34 (m, 2H), 3.50-3.25 (m, 2H), 3.16-2.89 (m, 2H), 2.76-2.16 (m, 5H), 2.05-1.87 (m, 1H).

[2080] Example 277. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(8-methyl-6-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2081] The title compound (122.9 mg, 40.3 %) was prepared in the same fashion as Example 28, except that (S)-8-methyl-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride (239.9 mg, 0.80 mmol) prepared in Reference Example 136 was used instead of (S)-N-(pyrrolidin-3 -yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 600MHz) δ 8.63 (s, 1H), 7.88 (d, 1H), 7.27-7.24 (m, 1H), 6.95 (d, 1H), 6.50 (s, 1H), 5.50-5.25 (m, 1H), 5.20-4.90 (m, 1H), 4.20-4.05 (m, 2H), 4.00-3.70 (m, 1H), 3.55-3.30 (m, 2H), 3.15-3.00 (m, 2H), 2.95-2.75 (m, 2H), 2.71 (s, 3H), 2.71-2.60 (m, 1H), 2.50- 2.20 (m, 3H), 1.95-1.80 (m, 1H)

[2082] Example 278. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(8-fluoro-6-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2083] The title compound (222.3 mg, 48.9 %) was prepared in the same fashion as Example 28, except that (S)-8-fluoro-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride (358.9 mg, 1.18 mmol) prepared in Reference Example 137 was used instead of (S)-N-(pyrrolidin-3 -yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 600MHz) δ 8.62 (s, 1H), 7.89 (d, 1H), 7.29 (d, 1H), 6.80 (t, 1H), 6.44 (s, 1H), 5.50-5.35 (m, 1H), 5.10-4.90 (m, 1H), 4.20-4.05 (m, 2H), 3.95-3.70 (m, 1H), 3.60- 3.30 (m, 2H), 3.15-2.95 (m, 2H), 2.90-2.60 (m, 3H), 2.50-2.20 (m, 3H), 1.90-1.75 (m, 1H)

[2084] Example 279. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(8-chloro-6-quinolyl)amino]py rrolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2085] The title compound (314.5 mg, 86.0 %) was prepared in the same fashion as Example 28, except that (S)-8-chloro-N-(pyrrolidin-3-yl)quinolin-6-amine hydrochloride (291.8 mg, 0.91 mmol) prepared in Reference Example 138 was used instead of (S)-N-(pyrrolidin-3 -yl)furo[3,2-c]pyridin-7- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 600MHz) δ 8.68 (s, 1H), 7.88 (d, 1H), 7.29-7.22 (m, 2H), 6.54 (s, 1H), 5.50-5.25 (m, 1H), 5.10-4.85 (m, 1H), 4.20-4.00 (m, 2H), 3.95-3.65 (m, 1H), 3.55-3.25 (m, 2H), 3.10-2.90 (m, 2H), 2.90-2.55 (m, 3H), 2.45-2.20 (m, 3H), 1.90-1.75 (m, 1H)

[2086] Example 280. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[[8-(trifluoromethyl)-6- quinolyl]amino]pyrrolidin- 1 -yl]acetyl]pyrrolidine-2-carbonitrile

[2087] The title compound (72.1 mg, 27.3 %) was prepared in the same fashion as Example 28, except that (S)-N-(pyrrolidin-3-yl)-8-(trifluoromethyl)quinolin-6-amine hydrochloride (215.7 mg, 0.61 mmol) prepared in Reference Example 139 was used instead of (S)-N-(pyrrolidin-3 -yl)furo[3,2- c]pyridin-7-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 600MHz) δ 8.75 (s, 1H), 7.94 (d, 1H), 7.45 (d, 1H), 7.34 (t, 1H), 6.77 (s, 1H), 5.50-5.35 (m, 1H), 5.10-4.90 (m, 1H), 4.20-4.05 (m, 2H), 3.95-3.65 (m, 1H), 3.60-3.30 (m, 2H), 3.15-3.00 (m, 2H), 2.90-2.60 (m, 3H), 2.50-2.20 (m, 3H), 1.95-1.80 (m, 1H)

[2088] Example 281. N-methyl-5-[[(3S)-1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrro lidin-1- yl] ethyl]py rrolidin-3 -yl] amino] quinoline -8 -carboxamide

[2089] The title compound (8.6 mg, 16.9 %) was prepared in the same fashion as Example 28, except that (S)-N-methyl-5-(pyrrolidin-3-ylamino)quinoline-8-carboxamide hydrochloride (41.0 mg, 0.12 mmol) prepared in Reference Example 140 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2- c]pyridin-7 -amine hydrochloride. Ή NMR (CDCI ₃ , 400 MHz) 10 δ.95 (t, 1H), 8.87-8.85 (m, 1H), 8.72 (dd, 1H), 8.42 (dd, 1H), 7.41-7.36 (m, 1H), 6.61 (dd, 1H), 5.93-5.62 (m, 1H), 5.53-5.27 (m, 1H), 4.94 (dd, 1H), 4.30-4.22 (m, 1H), 4.16-4.07 (m, 1H), 3.92-3.69 (m, 2H), 3.67-3.38 (m, 2H), 3.29-3.03 (m, 6H), 2.75-2.63 (m, 2H), 2.54-2.20 (m, 1H), 2.10-1.95 (m, 1H).

[2090] Example 282. N,N-dimethyl-5-[[(3S)-1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-p yrrolidin-1- yl] ethyl]py rrolidin-3 -yl] amino] quinoline -8 -carboxamide

[2091] The title compound (10.0 mg, 19.0 %) was prepared in the same fashion as Example 28, except that (S)-N,N-dimethyl-5-(pyrrolidin-3-ylamino)quinoline-8-carboxa mide hydrochloride (42.7 mg, 0.12 mmol) prepared in Reference Example 141 was used instead of (S)-N-(pyrrolidin-3- yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MH 8z.)8 δ6-8.84 (m, 1H), 8.31- 8.26 (m, 1H), 7.46 (d, 1H), 7.30-7.28 (m, 1H), 6.49-6.44 (m, 1H), 5.62-4.80 (m, 3H), 4.16-4.14 (m, 1H), 3.96-3.63 (m, 2H), 3.60-3.25 (m, 2H), 3.22 (d, 3H), 3.17-2.87 (m, 2H), 2.80 (d, 3H), 2.62-2.20 (m, 5H), 2.03-1.95 (m, 1H).

[2092] Example 283. N-phenyl-5-[[(3S)-1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrro lidin-1- yl] ethyl]py rrolidin-3 -yl] amino] quinoline-8 -carboxamide

[2093] The title compound (12.0 mg, 21.2 %) was prepared in the same fashion as Example 28, except that (S)-N-phenyl-5-(pynOlidin-3-ylamino)quinoline-8-carboxamide hydrochloride (48.5 mg, 0.12 mmol) prepared in Reference Example 142 was used instead of (S)-N-(pyrrolidin-3- yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MH 8z.)8 δ8 (t, 1H), 8.74-8.71 (m, 1H), 7.43 (d, 1H), 7.85 (d, 2H), 7.39-7.35 (m, 3H), 7.11-7.07 (m, 1H), 6.57 (dd, 1H), 6.28-5.81 (m, 1H), 5.43-5.24 (m, 1H), 4.91 (dd, 1H), 4.23-4.15 (m, 1H), 4.03-3.15 (m, 5H), 3.08-2.99 (m, 2H), 2.75-2.18 (m, 5H), 2.03-1.95 (m, 1H).

[2094] Example 284. N-methyl-N-phenyl-5-[[(3S)-1-[2-oxo-2-[(2S,4S)-2-cyano-4-flu oro- pyrrolidin-1-yl]ethyl]pynOlidin-3-yl]amino]quinoline-8-carbo xamide

[2095] The title compound (17.6 mg, 28.6 %) was prepared in the same fashion as Example 28, except that (S)-N-methyl-N-phenyl-5-(pyrrolidin-3-ylamino)quinoline-8-ca rboxamide hydrochloride (50.21 mg, 0.12 mmol) prepared in Reference Example 143 was used instead of (S)-N-(pyrrolidin-3- yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MH 8z.)8 δ5-8.81 (m, 1H), 8.21- 8.15 (m, 1H), 7.29-7.00 (m, 7H), 6.27-6.22 (m, 1H), 5.38-5.15 (m, 1H), 4.89 (dd, 1H), 4.02-3.66 (m, 2H), 3.59-3.50 (m, 2H), 3.42 (s, 3H), 3.35-3.22 (m, 2H), 3.06-2.79 (m, 2H), 2.61-1.98 (m, 5H), 1.89- 1.78 (m, 1H).

[2096] Example 285. N-benzyl-N-methyl-5-[[(3S)-1-[2-oxo-2-[(2S,4S)-2-cyano-4-flu oro- pyrrolidin-1-yl]ethyl]pyrrolidin-3-yl]amino]quinoline-8-carb oxamide [2097] The title compound (11.0 mg, 17.8 %) was prepared in the same fashion as Example 28, except that (S)-N-benzyl-N-methyl-5-(pyrrolidin-3-ylamino)quinoline-8-ca rboxamide hydrochloride (51.89 mg, 0.12 mmol) prepared in Reference Example 144 was used instead of (S)-N-(pyrrolidin-3- yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz 8).8 δ8-8.85 (m, 1H), 8.35- 8.27 (m, 1H), 7.51 (t, 2H), 7.37 (t, 1H), 7.29-7.17 (m, 4H), 6.48-6.40 (m, 1H), 5.79-5.13 (m, 3H), 5.05-4.76 (m, 1H), 4.40-4.11 (m, 2H), 3.84-3.58 (m, 2H), 3.42 (s, 3H), 3.35-3.20 (m, 2H), 3.08 (s, 2H), 3.02-2.16 (m, 5H), 1.98-1.86 (m, 1H).

[2098] Example 286. N-methyl-5-[[(3S)-1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1- yl] ethyl]py rrolidin-3 -yl] amino] quinoline -8 -carboxamide

[2099] The title compound (10.1 mg, 29.7 %) was prepared in the same fashion as Example 5, except that (S)-N-methyl-5-(pyrrolidin-3-ylamino)quinoline-8-carboxamide hydrochloride (29.0 mg, 0.084 mmol) prepared in Reference Example 140 was used instead of (S)-N-(pyrrolidin-3- yl)quinoline-4-amine hydrochloride. ¹ H NMR (CDCI ₃ . 400 MHz) 10 δ.98-10.94 (m, 1H), 8.85-8.83 (m, 1H), 8.70 (dd, 1H), 8.48 (dd, 1H), 7.40-7.34 (m, 1H), 6.60 (dd, 1H), 6.39-5.65 (m, 1H), 4.78-4.73 (m, 1H), 4.27-4.24 (m, 1H), 3.66-3.42 (m, 3H), 3.29-3.01 (m, 5H), 2.79-2.70 (m, 1H), 2.49-2.36 (m, 1H), 2.33-1.96 (m, 7H)

[2100] Example 287. N-phenyl-5-[[(3S)-1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1- yl] ethyl]py rrolidin-3 -yl] amino] quinoline -8 -carboxamide

[2101] The title compound (4.4 mg, 12.1 %) was prepared in the same fashion as Example 5, except that (S)-N-phenyl-5-(pynOlidin-3-ylamino)quinoline-8-carboxamide hydrochloride (30.0 mg, 0.074 mmol) prepared in Reference Example 142 was used instead of (S)-N-(pyrrolidin-3- yl)quinoline-4-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) 8. δ88 (t, 1H), 8.72 (dd, 1H), 8.43 (dd, 1H), 7.85 (d, 2H), 7.39-7.35 (m, 3H), 7.09 (t, 1H), 6.57 (dd, 1H), 6.28-5.81 (m, 1H), 5.44-5.24 (m, 1H), 4.91 (dd, 1H), 4.23-4.15 (m, 1H), 4.04-3.17 (m, 5H), 3.08-2.99 (m, 2H), 2.75-2.60 (m, 2H), 2.39-2.18 (m, 2H), 2.05-1.93 (m, 1H)

[2102] Example 288. N-benzyl-N-methyl-5-[[(3S)-1-[2-oxo-2-[(2S)-2-cyanopyrrolidi n-1- yl] ethyl]py rrolidin-3 -yl] amino] quinoline -8 -carboxamide

[2103] The title compound (6.89 mg, 11.5 %) was prepared in the same fashion as Example 5, except that (S)-N-benzyl-N-methyl-5-(pyrrolidin-3-ylamino)quinoline-8-ca rboxamide hydrochloride

(52.0 mg, 0.12 mmol) prepared in Reference Example 144 was used instead of (S)-N-(pyrrolidin-3- yl)quinoline-4-amine hydrochloride. ¹ H NMR (CDCI ₃ . 400 MHz) 8. δ91-8.88 (m, 1H), 8.33 (dt, 1H),

7.53 (dd, 2H), 7.40 (t, 1H), 7.33-7.25 (m, 2H), 7.23-7.19 (m, 2H), 6.52-6.43(m, 1H), 5.80-5.32 (m,

1H), 4.99-4.74 (m, 1H), 4.38-4.32 (m, 1H), 4.22-4.13 (m, 1H), 3.63-3.43 (m, 4H), 3.10 (s, 3H), 2.99-

2.94 (m, 1H), 2.70-2.66 (m, 3H), 2.45-1.92 (m, 7H). [2104] Example 289. N-methyl-N-phenyl-5-[[(3S)-1-[2-oxo-2-[(2S)-2-cyanopyrrolidi n-1- yl] ethyl]py rrolidin-3 -yl] amino] quinoline -8 -carboxamide

[2105] The title compound (5.9 mg, 10.2 %) was prepared in the same fashion as Example 5, except that (S)-N-methyl-N-phenyl-5-(pyrrolidin-3-ylamino)quinoline-8-ca rboxamide hydrochloride (50.3 mg, 0.12 mmol) prepared in Reference Example 143 was used instead of (S)-N-(pyrrolidin-3- yl)quinoline-4-amine hydrochloride. ¹ H NMR (CDCI ₃ ,. 400 MHz 8).8 δ6-8.82 (dd, 1H), 8.38-8.28 (m, 2H), 7.29-7.25 (m, 2H), 7.05-7.00 (m, 4H), 6.18 (d, 1H), 4.76 (d, 1H), 4.09 (br s, 1H), 3.98-3.70 (m, 2H), 3.66- 3.15 (m, 7H), 3.03 (br s, 1H), 2.32-2.07 (m, 4H), 2.02-1.96 (m, 1H).

[2106] Example 290. N-(3-pyridyl)-5-[[(3S)-1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1- yl] ethyl]py rrolidin-3 -yl] amino] quinoline -8 -carboxamide

[2107] The title compound (21.8 mg, 50.5 %) was prepared in the same fashion as Example 5, except that (S)-N-(pyridin-3-yl)-5-(pyrrolidin-3-ylamino)quinoline-8-car boxamide hydrochloride (36.9 mg, 0.09 mmol) prepared in Reference Example 145 was used instead of (S)-N-(pyrrolidin-3- yl)quinoline-4-amine hydrochloride. ¹ H NMR (CDCI ₃ ,. 400 MHz 8).9 δ1-8.88 (m, 1H), 8.86-8.84 (m, 1H), 8.75-8.71 (m, 1H), 8.67 (d, 0.7H), 8.46-8.44 (m, 1.3H), 8.31 (d, 1H), 7.44-7.39 (m, 1H), 7.31- 7.28 (m, 1H), 6.87 (d, 0.7H), 6.62 (d, 1H), 5.95 (d, 0.3H), 4.78-4.70 (m, 1H), 4.24 (s, 1H), 3.67-3.40 (m, 3H), 3.17-3.11 (m, 2H), 2.81-2.69 (m, 1H), 2.49-2.12 (m, 8H), 2.04-1.97 (m, 1H).

[2108] Example 291. N-(4-pyridyl)-5-[[(3S)-1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1- yl] ethyl]py rrolidin-3 -yl] amino] quinoline -8 -carboxamide

[2109] The title compound (11.2 mg, 19.8 %) was prepared in the same fashion as Example 5, except that (S)-N-(pyridin-4-yl)-5-(pyrrolidin-3-ylamino)quinoline-8-car boxamide hydrochloride (48.7 mg, 0.12 mmol) prepared in Reference Example 146 was used instead of (S)-N-(pyrrolidin-3- yl)quinoline-4-amine hydrochloride. ¹ H NMR (CDCl _’ ,. 400 MHz 8).9 δ1-8.87 (m, 1H), 8.74-8.67 (m, 1.7H), 8.49-8.44 (dd, 2.3H), 7.76 (d, 2H), 7.45-7.39 (m, 1H), 7.00 (d, 0.7H), 6.62 (d, 1H), 6.01 (d, 0.3H), 4.78-4.70 (m, 1H), 4.25 (s, 1H), 3.67-3.40 (m, 3H), 3.19-3.12 (m, 2H), 2.82-2.70 (m, 1H), 2.49-2.08 (m, 8H), 2.04-1.97 (m, 1H).

[2110] Example 292. N-(4-pyridyl)-5-[[(3S)-1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro- pyrrolidin-1- yl] ethyl]pyrrolidin-3 -yl] amino] quinoline -8 -carboxamide

[2111] The title compound (8.4 mg, 14.3 %) was prepared in the same fashion as Example 28, except that (S)-N-(pyridin-4-yl)-5-(pyrrolidin-3-ylamino)quinoline-8-car boxamide hydrochloride (48.7 mg, 0.12 mmol) prepared in Reference Example 146 was used instead of (S)-N-(pyrrolidin-3- yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz 8).9 δ2-8.89 (m, 1H), 8.72 (t, 1H), 8.59-8.43 (m, 3H), 7.76-7.74 (m, 2H), 7.45-7.41 (m, 1H), 6.63 (d, 1H), 6.54 (d, 0.6H), 5.97 (d, 0.4H), 5.52-5.28 (m, 1H), 4.93 (dd, 1H), 4.30-4.25 (m, 1H), 4.01-3.39 (m, 4H), 3.29-3.06 (m, 3H), 2.79-2.65 (m, 2H), 2.53-2.24 (m, 2H), 2.11-1.99 (m, 1H).

[2112] Example 293. N-(2-pyridyl)-5-[[(3S)-1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1- yl] ethyl]py rrolidin-3 -yl] amino] quinoline -8 -carboxamide

[2113] The title compound (4.3 mg, 7.6 %) was prepared in the same fashion as Example 5, except that (S)-N-(pyridin-2-yl)-5-(pyrrolidin-3-ylamino)quinoline-8-car boxamide hydrochloride (48.7 mg, 0.12 mmol) prepared in Reference Example 147 was used instead of (S)-N-(pyrrolidin-3- yl)quinoline-4-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) 9. δ06 (td, 1H), 8.80 (dd, 1H), 8.64 (dd, 0.6H), 8.54 (d, 1H), 8.44-8.40 (m, 1.4H), 7.76-7.71 (m, 1H), 7.48-7.41 (m, 1H), 7.04-7.00 (m, 1H), 6.74-6.65 (m, 1.6H), 5.85 (d, 0.4H), 4.81-4.70 (m, 1H), 4.30-4.28 (m, 1H), 3.68-3.43 (m, 4H),

3.28-3.04 (m, 3H), 2.82-2.76 (m, 1H), 2.51-2.11 (m, 6H), 2.03-2.00 (m, 1H).

[2114] Example 294. N-(2-pyridyl)-5-[[(3S)-1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro- pyrrolidin-1- yl] ethyl]pyrrolidin-3 -yl] amino] quinoline -8 -carboxamide

[2115] The title compound (1.7 mg, 2.9 %) was prepared in the same fashion as Example 28, except that (S)-N-(pyridin-2-yl)-5-(pyrrolidin-3-ylamino)quinoline-8-car boxamide hydrochloride (48.7 mg, 0.12 mmol) prepared in Reference Example 147 was used instead of (S)-N-(pyrrolidin-3- yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MH 9z.)0 δ6 (td, 1H), 8.79 (dd, 1H), 8.54-8.40 (m, 3H), 7.76-7.71 (m, 1H), 7.45-7.41 (m, 1H), 7.04-7.00 (m, 1H), 6.65 (t, 1H), 6.28 (d, 0.5H), 5.85 (d, 0.5H), 5.51-5.28 (m, 1H), 4.93 (dd, 1H), 4.32-4.26 (m, 1H), 4.13-3.39 (m, 4H),

3.29-3.05 (m, 3H), 2.78-2.64 (m, 2H), 2.57-2.21 (m, 3H), 2.12-1.97 (m, 1H).

[2116] Example 295. N-(3-pyridyl)-5-[[(3S)-1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro- pyrrolidin-1- yl] ethyl]pyrrolidin-3 -yl] amino] quinoline -8 -carboxamide

[2117] The title compound (2.6 mg, 5.5 %) was prepared in the same fashion as Example 28, except that (S)-N-(pyridin-3-yl)-5-(pyrrolidin-3-ylamino)quinoline-8-car boxamide hydrochloride (36.9 mg, 0.09 mmol) prepared in Reference Example 145 was used instead of (S)-N-(pyrrolidin-3- yl)furo[3,2-c]pyridin-7-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz 8).9 δ6-8.95 (m, 1H), 8.86 (t, 1H), 8.80-8.77 (m, 1H), 8.59-8.45 (m, 2H), 8.33-8.32 (m, 1H), 7.49-7.44 (m, 1H), 7.34-7.30 (m, 1H), 6.67 (t, 1H), 6.38 (d, 0.5H), 5.89 (d, 0.5H), 5.54-5.30 (m, 1H), 4.94 (dd, 1H), 4.34-4.28 (m, 1H), 4.15-3.39 (m, 4H), 3.29-3.07 (m, 3H), 2.80-2.67 (m, 2H), 2.58-2.23 (m, 3H), 2.13-1.99 (m, 1H).

[2118] Example 296. N-[5-[[(3S)-1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1-yl]ethyl]py rrolidin-3- yl] amino] - 8-quinolyl] acetamide

[2119] The title compound (5.9 mg, 12.2 %) was prepared in the same fashion as Example 5, except that (S)-N-(5-(pynOlidin-3-ylamino)quinolin-8-yl)acetamide hydrochloride (41.2 mg, 0.12 mmol) prepared in Reference Example 148 was used instead of (S)-N-(pyrrolidin-3-yl)quinoline-4- amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) 9 δ.47 (s, 1H), 8.76-8.74 (m, 1H), 8.59-8.57 (m, 1H), 8.39-8.28 (m, 1H), 7.40-7.36 (m, 1H), 6.52 (t, 1H), 4.79-4.72 (m, 1H), 4.17-4.09 (m, 1H), 3.64- 3.58 (m, 1H), 3.54-3.23 (m, 3H), 3.09-2.91 (m, 3H), 2.74-2.59 (m, 1H), 2.47-2.02 (m, 9H), 1.96-1.86 (m, 1H).

[2120] Example 297. N-[5-[[(3S)-1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrrolidin- 1- yl] ethy l]pyrrolidin-3 -yl] amino] - 8 -quinolyl] acetamide

[2121] The title compound (3.9 mg, 7.6 %) was prepared in the same fashion as Example 28, except that (S)-N-(5-(pynOlidin-3-ylamino)quinolin-8-yl)acetamide hydrochloride (41.2 mg, 0.12 mmol) prepared in Reference Example 148 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2- c]pyridin-7-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) 9. δ47-9.46 (m, 1H), 8.77-8.74 (m,

1H), 8.56 (d, 1H), 8.33-8.29 (m, 1H), 7.39-7.36 (m, 1H), 6.52-6.48 (m, 1H), 5.45-5.21 (m, 1H), 5.01-

4.88 (m, 2H), 4.17-4.12 (m, 1H), 4.08-3.38 (m, 3H), 3.31-3.26 (m, 1H), 3.14-3.01 (m, 1H), 2.96-

2.89 (m, 1H), 2.71-2.54 (m, 2H), 2.47-2.18 (m, 5H), 2.00-1.83 (m, 1H).

[2122] Example 298. N-[5-[[(3S)-1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrrolidin- 1- yl] ethyl]py rrolidin-3 -yl] amino] -8 -quinoly l]benzamide

[2123] The title compound (9.8 mg, 16.8 %) was prepared in the same fashion as Example 28, except that (S)-N-(5-(pyrrolidin-3-ylamino)quinolin-8-yl)benzamide hydrochloride (48.5 mg, 0.12 mmol) prepared in Reference Example 149 was used instead of (S)-N-(pyrrolidin-3-yl)furo[3,2- c]pyridin-7-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) 10 δ.46 (s, 1H), 8.83-8.78 (m, 2H), 8.36-8.33 (m, 1H), 8.06-8.02 (m, 2H), 7.60-7.48 (m, 3H), 7.43-7.40 (m, 1H), 6.59 (t, 1H), 5.47-5.23 (m, 1H), 5.02-4.91 (m, 2H), 4.22-4.18 (m, 1H), 4.15-3.55 (m, 3H), 3.48-3.13 (m, 1H), 3.10-2.93 (m, 2H), 2.75-2.59 (m, 2H), 2.53-2.16 (m, 2.5H), 2.05-1.88 (m, 1.5H).

[2124] Example 299. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(6-methoxy-4-quinolyl)oxy]pyr rolidin-1- yl]acetyl]pyrrolidine-2-carbonitrile

[2125] (2S,4S)-1-(2-chloroacetyl)-4-fluoro-pyrrolidine-2-carbonitri le (152 mg, 0.80 mmol) prepared in Reference Example 2 was dissolved in anhydrous acetonitrile (4.0 mL). To the resulting solution, (S)-6-methoxy-4-(pyrrolidin-3-yloxy)quinoline hydrochloride (224 mg, 0.80 mmol) prepared in Reference Example 150, potassium carbonate (441 mg, 3.2 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol= 10/1, v/v) to give a title compound (76 mg, 24%). ¹ H NMR (CDCI ₃ , 600 MHz) δ 8.54 (d, 1H), 7.89 (d, 1H), 7.41 (s, 1H), 7.32 (d, 1H), 6.56 (d, 1H), 5.41- 5.17 (m, 1.3H), 5.12-5.00 (m, 1H), 4.91 (d, 0.7H), 4.17-4.04 (m, 1H), 3.91 (s, 3H), 3.79-3.29 (m, 3H), 3.23-3.09 (m, 1H), 3.07-2.89 (m, 2H), 2.75-2.54 (m, 2H), 2.48-2.40 (m, 1H), 2.32-2.09 (m, 2H)

[2126] Example 300. (2S,4S)-4-fluoro-1-[2-[(3S)-3-[(6,7-dimethoxy-4-quinolyl)oxy ] pyrrolidin- l-yl]acetyl]pyrrolidine-2-carbonitrile

[2127] (2S,4S)-1-(2-chloroacetyl)-4-fluoro-pyrrolidine-2-carbonitri le (71 mg, 0.37 mmol) prepared in Reference Example 2 was dissolved in anhydrous acetonitrile (2.0 mL). To the resulting solution, (S)-6,7-dimethoxy-4-(pyrrolidin-3-yloxy)quinoline hydrochloride (114 mg, 0.37 mmol) prepared in Reference Example 151, potassium carbonate (204 mg, 1.5 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with dichloromethane. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol= 10/1, v/v) to give a title compound (40 mg, 25%). ¹ H NMR (CDCI ₃ , 600 MHz) 8 δ.52 (d, 1H), 7.39 (s, 1H), 7.35 (s, 1H), 6.53 (d, 1H), 5.46-5.20 (m, 1.4H), 5.14-5.01 (m, 1H), 4.94 (d, 0.6H), 4.23-4.12 (m, 1H), 4.01 (d, 6H), 3.96-3.60 (m, 2H), 3.47-2.91 (m, 4H), 2.80-2.57 (m, 2H), 2.50-2.40 (m, 1H), 2.37-2.10 (m, 2H)

[2128] Example 301. N-[1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1-yl]ethyl]-4-piperidy l]quinoline-8- carboxamide

[2129] The title compound (424.3 mg, 86.9 %) was prepared in the same fashion as Example 110, except that N-(piperidin-4-yl)quinoline-8-carboxamide hydrochloride (409.7 mg, 1.25 mmol) prepared in Reference Example 152 was used instead ofN-(piperidin-4-yl)quinoline-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 11.47 (s, 1H), 8.92 (d, 1H), 8.83 (d, 1H), 8.28 (d, 1H), 7.96 (d, 1H), 7.66 (t, 1H), 7.49 (t, 1H), 5.40-4.70 (m, 1H), 4.30-4.10 (m, 1H), 3.85-3.50 (m, 2H), 3.50-3.10 (m, 2H), 3.00-2.75 (m, 2H), 2.55-2.20 (m, 4H), 2.20-2.00 (m, 4H), 2.00-1.70 (m, 2H)

[2130] Example 302. (2S)-1-[2-[4-[ethyl(3-quinolyl)amino]-1-piperidyl]acetyl]pyr rolidine-2- carbonitrile

[2131] The title compound (132.5 mg, 38.4 %) was prepared in the same fashion as Example 121, except that N-ethyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride (230.4 mg, 0.88 mmol) prepared in Reference Example 153 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) 8. δ67 (d, 1H), 7.92-7.90 (m, 1H), 7.61-7.59 (m, 1H), 7.41-7.36 (m, 2H), 7.14 (d, 1H), 5.07-4.73 (m, 1H), 3.74-3.61 (m, 2H), 3.56-3.47 (m, 1H), 3.44- 3.38 (m, 2H), 3.36-3.19 (m, 2H), 3.08-2.96 (m, 2H), 2.33-2.03 (m, 7H), 1.90-1.85 (m, 3H), 1.21 (t, 3H). [2132] Example 303. (2S)-1-[2-[4-[ethyl(6-quinolyl)amino]-1-piperidyl]acetyl]pyr rolidine-2- carbonitrile

[2133] The title compound (274.8 mg, 79.7 %) was prepared in the same fashion as Example 121, except that N-ethyl-N-(piperidin-4-yl)quinolin-6-amine hydrochloride (230.4 mg, 0.88 mmol) prepared in Reference Example 154 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDC1 ₃ , 400 MHz) 8. δ54 (dd, 1H), 7.90-7.86 (m, 2H), 7.30 (dd, 1H), 7.19 (q, 1H), 6.77 (d, 1H), 5.06-4.79 (m, 1H), 3.71-3.58 (m, 2H), 3.53-3.43 (m, 1H), 3.39-3.33 (m, 2H), 3.29-3.12 (m, 2H), 3.04-2.93 (m, 2H), 2.31-1.96 (m, 6H), 1.86-1.78 (m, 4H), 1.18 (t, 3H).

[2134] Example 304. (2S,4S)-1-[2-[4-[ethyl(3-quinolyl)amino]-1-piperidyl]acetyl] -4-fluoro- pyrrolidine-2-carbonitrile

[2135] The title compound (12.4 mg, 27.5 %) was prepared in the same fashion as Example 131, except that N-ethyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride (36.1 mg, 0.11 mmol) prepared in Reference Example 153 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5-amine hydrochloride. ¹ H NMR (CDC1 ₃ , 400 MHz 8). δ69 (d, 1H), 7.95-7.92 (m, 1H), 7.63-7.61 (m, 1H), 7.44-7.40 (m, 2H), 7.17 (s, 1H), 5.51-4.94 (m, 2H), 4.21-4.12 (m, 1H), 4.02-3.71 (m, 2H), 3.47-3.40 (m, 3H), 3.30-3.19 (m, 2H), 3.12-2.99 (m, 2H), 2.80-2.64 (m, 1H), 2.50-2.24 (m, 3H), 1.89-1.88 (m, 3H), 1.23 (t, 3H).

[2136] Example 305. (2S,4S)-1-[2-[4-[ethyl(5-quinolyl)amino]-1-piperidyl]acetyl] -4-fluoro- pyrrolidine-2-carbonitrile

[2137] The title compound (39.8 mg, 88.5 %) was prepared in the same fashion as Example 131, except that N-ethyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride (36.1 mg, 0.11 mmol) prepared in Reference Example 155 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) 8 δ.84 (d, 1H), 8.63 (m, 1H), 7.83 (d, 1H), 7.61 (t, 1H), 7.35 (q, 1H), 7.26 (d 1H), 5.44-4.83 (m, 2H), 4.10-3.83 (m, 1H), 3.79-3.66 (m, 1H), 3.37-3.06 (m,

4H), 2.99-2.94 (m, 1H), 2.90-2.76 (m, 2H), 2.69-2.20 (m, 2H), 2.07-2.00 (m, 2H), 1.81-1.59 (m, 4H), 0.86 (t, 3H).

[2138] Example 306. (2S,4S)-1-[2-[4-[ethyl(6-quinolyl)amino]-1-piperidyl]acetyl] -4-fluoro- pyrrolidine-2-carbonitrile

[2139] The title compound (35.4 mg, 78.6 %) was prepared in the same fashion as Example 131, except that N-ethyl-N-(piperidin-4-yl)quinolin-6-amine hydrochloride (36.1 mg, 0.11 mmol) prepared in Reference Example 154 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) 8 δ.54 (dd, 1H), 7.90 (s, 1H), 7.87 (s, 1H), 7.32-7.29 (m, 1H), 7.20 (q, 1H), 6.78-6.77 (m, 1H), 5.44-4.87 (m, 2H), 4.13-4.04 (m, 1H), 3.95-3.64 (m, 2H), 3.39- 3.34 (m, 2H), 3.26-3.13 (m, 2H), 3.06-2.94 (m, 2H), 2.36-2.22 (m, 3H), 1.86-1.82 (m, 4H), 1.18 (t, 3H).

[2140] Example 307. (2S)-1-[2-[4-[methyl(2-quinolyl)amino]-1-piperidyl]acetyl]py rrolidine-2- carbonitrile

[2141] The title compound (45.7 mg, 13.7 %) was prepared in the same fashion as Example 121, except that N-methyl-N-(piperidin-4-yl)quinolin-2-amine hydrochloride (276.5 mg, 0.88 mmol) prepared in Reference Example 156 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) 7 δ.86 (d, 1H), 7.67 (d, 1H), 7.58 (d, 1H), 7.51 (t, 1H), 7.17 (t, 1H), 6.88 (d, 1H), 5.19-4.71 (m, 2H), 3.81-3.73 (m, 2H), 3.59-3.55 (m, 1H), 3.42-3.22 (m, 2H), 3.08-3.04 (m, 4H), 2.39 (t, 2H), 2.29-2.11 (m, 4H), 1.96-1.90 (m, 2H), 1.76 (d, 2H).

[2142] Example 308. (2S)-1-[2-[4-[methyl(4-quinolyl)amino]-1-piperidyl]acetyl]py rrolidine-2- carbonitrile

[2143] The title compound (79.5 mg, 23.9 %) was prepared in the same fashion as Example 121, except that N-methyl-N-(piperidin-4-yl)quinolin-4-amine hydrochloride (276.5 mg, 0.88 mmol) prepared in Reference Example 157 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDC1 ₃ , 400 MHz) 8. δ67 (d, 1H), 8.04 (d, 1H), 7.94 (d, 1H), 7.65 (t, 1H), 7.46 (t, 1H), 6.85 (d, 1H), 5.09-4.75 (m, 1H), 3.70-3.48 (m, 3H), 3.31-3.12 (m, 4H), 2.89 (s, 3H), 2.29-2.01 (m, 8H), 1.80 (d, 2H).

[2144] Example 309. (2S)-1-[2-[4-[methyl(5-quinolyl)amino]-1-piperidyl]acetyl]py rrolidine-2- carbonitrile

[2145] The title compound (220.8 mg, 66.4 %) was prepared in the same fashion as Example 121, except that N-methyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride (276.5 mg, 0.88 mmol) prepared in Reference Example 158 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDC1 ₃ , 400 MHz) 8. δ88 (d, 1H), 8.51 (d, 1H), 7.80 (d, 1H), 7.62 (t, 1H), 7.38 (q, 1H), 7.20 (d, 1H), 5.18-4.72 (m, 1H), 3.72-3.48 (m, 2H), 3.33-3.02 (m, 3H), 2.95-2.81 (m, 5H), 2.34-2.06 (m, 6H), 2.01-1.80 (m, 4H).

[2146] Example 310. (2S)-1-[2-[4-[methyl(6-quinolyl)amino]-1-piperidyl]acetyl]py rrolidine-2- carbonitrile

[2147] The title compound (171.0 mg, 51.4 %) was prepared in the same fashion as Example 121, except that N-methyl-N-(piperidin-4-yl)quinolin-6-amine hydrochloride (276.5 mg, 0.88 mmol) prepared in Reference Example 159 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDC1 ₃ , 400 MHz) 8. δ62 (d, 1H), 7.93 (d, 2H), 7.42 (d, 1H), 7.26 (q, 1H), 6.85 (d, 1H), 5.11-4.75 (m, 1H), 3.79-3.63 (m, 2H), 3.58-3.49 (m, 1H), 3.38-3.20 (m, 2H), 3.16- 2.97 (m, 2H), 3.29 (s, 3H), 2.35-2.08 (m, 7H), 1.99-1.88 (m, 1H), 1.78-1.75 (m, 2H).

[2148] Example 311. (2S)-1-[2-[4-[methyl(7-quinolyl)amino]-1-piperidyl]acetyl]py rrolidine-2- carbonitrile

[2149] The title compound (35.8 mg, 10.7 %) was prepared in the same fashion as Example 121, except that N-methyl-N-(piperidin-4-yl)quinolin-7-amine hydrochloride (276.5 mg, 0.88 mmol) prepared in Reference Example 160 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.73 (d, 1H), 7.96 (d, 1H), 7.64 (d, 1H), 7.22 (d, 2H), 7.10 (q, 1H), 5.14-4.77 (m, 1H), 3.86-3.55 (m, 3H), 3.39-3.00 (m, 4H), 2.94 (s, 3H), 2.45-2.09 (m, 6H), 2.03-1.89 (m, 2H), 1.81-1.78 (m, 2H).

[2150] Example 312. (2S)-1-[2-[4-[furo[3,2-c]pyridin-7-yl(methyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2151] The title compound (139.4 mg, 43.1 %) was prepared in the same fashion as Example 121, except that N-methyl-N-(piperidin-4-yl)furo[3,2-c]pyridin-7-amine hydrochloride (267.7 mg, 0.88 mmol) prepared in Reference Example 161 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline- 5 -amine hydchloride. ¹ H NMR (CDC1 ₃ , 400 MHz) δ 8.46 (s, 1H), 7.98 (s, 1H), 7.63 (s, 1H), 6.81 (s, 1H), 5.17-4.75 (m, 1H), 3.96-3.90 (m, 1H), 3.76-3.64 (m, 1H), 3.57-3.51 (m, 1H), 3.39-3.19 (m, 2H), 3.04-2.95 (m, 5H), 2.36-1.92 (m, 8H), 1.81-1.78 (m, 2H).

[2152] Example 313. (2S)-1-[2-[4-[5-isoquinolyl(methyl)amino]-1-piperidyl]acetyl ]pyrrolidine-

2-carbonitrile

[2153] The title compound (28.4 mg, 8.5 %) was prepared in the same fashion as Example 121, except that N-methyl-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride (267.7 mg, 0.88 mmol) prepared in Reference Example 162 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydchloride. ¹ H NMR (MeOD ₄ , 400 MHz) δ 9.18 (s, 1H), 8.42 (d, 1H), 8.06 (d, 1H), 7.75 (d, 1H), 7.64-7.51 (m, 2H), 4.74-4.64 (m, 1H), 3.73-3.50 (m, 2H), 3.26-2.85 (m, 8H), 2.22-2.09 (m, 6H), 1.95-1.80 (m, 4H).

[2154] Example 314. (2S)-1-(2-(4-(isoquinolin-6-yl(methyl)amino)piperidin-1- yl)acetyl)pyrrolidine-2-carbonitrile

[2155] The title compound (146.1 mg, 44.0 %) was prepared in the same fashion as Example 121, except that N-methyl-N-(piperidin-4-yl)isoquinolin-6-amine hydrochloride (267.7 mg, 0.88 mmol) prepared in Reference Example 163 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydchloride. ¹ H NMR (MeOD ₄ , 400 MHz) δ 8.84 (s, 1H), 8.13 (d, 1H), 7.86 (d, 1H), 7.51 (d, 1H), 7.41-7.38 (m, 1H), 6.92 (s, 1H), 5.17-4.76 (m, 1H), 3.96-3.90 (m, 1H), 3.78-3.73 (m, 1H), 3.61- 3.53 (m, 1H), 3.45-3.22 (m, 3H), 3.10-3.08 (m, 2H), 2.96 (s, 3H), 2.39-2.33 (m, 2H), 2.28-2.12 (m, 4H), 2.05-1.96 (m, 1H), 1.74-1.71 (m, 2H).

[2156] Example 315. (2S,4S)-4-fluoro-1-[2-[4-[methyl(6-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2157] The title compound (4.4 mg, 10.2 %) was prepared in the same fashion as Example 131, except that N-methyl-N-(piperidin-4-yl)quinolin-6-amine hydrochloride (34.5 mg, 0.11 mmol) prepared in Reference Example 159 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.62 (d, 1H), 7.94 (d, 2H), 7.41 (d, 1H), 7.27 (t, 1H), 6.85 (s, 1H), 5.49-4.93 (m, 2H), 4.21-3.91 (m, 1H), 3.87-3.75 (m, 2H), 3.46-3.18 (m, 2H), 3.11- 2.98 (m, 2H), 2.91 (s, 3H), 2.80-2.63 (m, 1H), 2.40-2.24 (m, 3H), 2.15-1.90 (m, 2H), 1.79-1.76 (m, 2H).

[2158] Example 316. (2S,4S)-4-fluoro-1-[2-[4-[methyl(3-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2159] The title compound (12.0 mg, 27.5 %) was prepared in the same fashion as Example 131, except that N-methyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride (34.5 mg, 0.11 mmol) prepared in Reference Example 164 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDC1 ₃ , 400 MHz) δ 8.76 (d, 1H), 7.97-7.95 (m, 1H), 7.66-7.64 (m, 1H), 7.46-7.43 (m, 2H), 7.22 (d, 1H), 5.51-4.95 (m, 2H), 4.22-3.92 (m, 1H), 3.88-3.75 (m, 2H), 3.46- 3.19 (m, 2H), 3.12-3.00 (m, 2H), 2.92 (s, 3H), 2.81-2.65 (m, 1H), 2.41-2.24 (m, 3H), 2.18-1.79 (m, 4H).

[2160] Example 317. (2S,4S)-4-fluoro-1-[2-[4-[3-isoquinolyl(methyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2161] The title compound (18.7 mg, 43.1 %) was prepared in the same fashion as Example 131, except that N-methyl-N-(piperidin-4-yl)isoquinolin-3-amine hydrochloride (34.5 mg, 0.11 mmol) prepared in Reference Example 165 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDC1 ₃ , 400 MHz) δ 8.75 (d, 1H), 7.95 (q, 1H), 7.65-7.63 (m, 1H), 7.43 (q, 2H), 7.21 (d, 1H), 5.49-4.93 (m, 2H), 4.20-3.91 (m, 1H), 3.87-3.73 (m, 2H), 3.45-3.17 (m, 2H), 3.11-2.99 (m, 2H), 2.91 (s, 3H), 2.76-2.63 (m, 1H), 2.40-2.24 (m, 3H), 2.13-1.77 (m, 4H).

[2162] Example 318. (2S,4S)-4-fluoro-1-[2-[4-[5-isoquinolyl(methyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2163] The title compound (28.4 mg, 65.4 %) was prepared in the same fashion as Example 131, except that N-methyl-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride (34.5 mg, 0.11 mmol) prepared in Reference Example 162 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDC1 ₃ , 400 MHz) δ 9.21 (s, 1H), 8.51 (dd, 1H), 7.91 (d, 1H), 7.65 (d, 1H), 7.52 (t, 1H), 7.33 (d, 1H), 5.47-4.91 (m, 2H), 4.18-3.90 (m, 1H), 3.85-3.72 (m, 1H), 3.42-3.13 (m, 3H), 2.99-2.86 (m, 2H), 2.82 (s, 3H), 2.78-2.61 (m, 1H), 2.38-1.82 (m, 7H).

[2164] Example 319. (2S,4S)-4-fluoro-1-[2-[4-[7-isoquinolyl(methyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2165] The title compound (14.0 mg, 32.1 %) was prepared in the same fashion as Example 131, except that N-methyl-N-(piperidin-4-yl)isoquinolin-7-amine hydrochloride (34.5 mg, 0.11 mmol) prepared in Reference Example 166 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 9.0 6(s, 1H), 8.27 (dd, 1H), 7.69 (d, 1H), 7.49 (d, 1H), 7.40 (d, 1H), 7.02 (s, 1H), 5.51-4.95 (m, 2H), 4.23-3.92 (m, 1H), 3.88-3.75 (m, 2H), 3.47-3.23 (m, 2H), 3.12-3.00 (m, 2H), 2.92 (s, 3H), 2.77-2.65 (m, 1H), 2.41-2.29 (m, 3H), 2.05-1.89 (m, 2H), 1.80-1.77 (m, 2H).

[2166] Example 320. (2S,4S)-4-fluoro-1-[2-[4-[8-isoquinolyl(methyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2167] The title compound (20.4 mg, 46.9 %) was prepared in the same fashion as Example 131, except that N-methyl-N-(piperidin-4-yl)isoquinolin-8-amine hydrochloride (34.5 mg, 0.11 mmol) prepared in Reference Example 166 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDC1 ₃ , 400 MHz) δ 9.51 (s, 1H), 8.45 (dd, 1H), 7.59-7.43 (m, 3H),

7.17 (d, 1H), 5.45-4.87 (m, 2H), 4.11-3.70 (m, 2H), 3.47-3.09 (m, 3H), 2.92-2.89 (m, 2H), 2.82 (s, 3H), 2.74-2.57 (m, 2H), 2.37-2.22 (m, 2H), 2.09-2.03 (m, 2H), 1.88-1.81 (m, 2H).

[2168] Example 321. (2S)-1-[2-[4-[3-isoquinolyl(methyl)amino]-1-piperidyl]acetyl ]pyrrolidine-

2-carbonitrile

[2169] The title compound (97.5 mg, 28.0 %) was prepared in the same fashion as Example 121, except that N-methyl-N-(piperidin-4-yl)isoquinolin-3-amine hydrochloride (276.5 mg, 0.88 mmol) prepared in Reference Example 165 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.75 (d, 1H), 7.96-7.93 (m, 1H), 7.65-7.63 (m, 1H), 7.44-7.42 (m, 2H), 7.20 (d, 1H), 5.10-4.76 (m, 1H), 3.79-3.65 (m, 2H), 3.57-3.51 (m, 1H), 3.38-

3.17 (m, 2H), 3.12-3.07 (m, 2H), 2.91 (s, 3H), 2.37-1.79 (m, 10H).

[2170] Example 322. (2S)-1-[2-[4-[methyl-(6-methyl-3-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2171] The title compound (75.9 mg, 22.0 %) was prepared in the same fashion as Example 121, except that N,6-dimethyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride (344.5 mg, 0.88 mmol) prepared in Reference Example 168 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.65 (d, 1H), 7.82 (d, 1H), 7.39 (s, 1H), 7.24 (d, 1H), 7.11 (d, 1H), 5.09-4.75 (m, 1H), 4.21-3.96 (m, 2H), 3.67-3.50 (m, 3H), 3.33-3.18 (m, 2H), 3.06- 2.96 (m, 2H), 2.88 (s, 3H), 2.47 (s, 3H), 2.30-2.06 (m, 5H), 1.98-1.87 (m, 3H).

[2172] Example 323. (S)-1-(2-(4-((6-methoxyquinolin-3-yl)(methyl)amino)piperidin -1- yl)acetyl)pyrrolidine-2-carbonitrile

[2173] The title compound (55.8 mg, 15.5 %) was prepared in the same fashion as Example 121, except that 6-methoxy-N-methyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride (344.5 mg, 0.88 mmol) prepared in Reference Example 169 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline- 5 -amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.54 (d, 1H), 7.80 (d, 1H), 7.09 (d, 1H), 7.04 (dd, 1H), 6.69 (d, 1H), 5.06-4.72 (m, 1H), 3.87 (s, 3H), 3.75-3.60 (m, 2H), 3.55-3.46 (m, 1H), 3.34- 3.13 (m, 2H), 3.05-2.94 (m, 2H), 2.87 (s, 3H), 2.36-1.86 (m, 8H), 1.74 (d, 2H).

[2174] Example 324. (2S)-1-[2-[4-[(7-methoxy-3-quinolyl)-methyl-amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2175] The title compound (59.0 mg, 16.4 %) was prepared in the same fashion as Example 121, except that 7-methoxy-N-methyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride (344.5 mg, 0.88 mmol) prepared in Reference Example 170 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline- 5 -amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8. 68 (d, 1H), 7.55 (d, 1H), 7.32 (d, 1H), 7.24 (d, 1H), 7.13 (d, 1H), 5.13-4.78 (m, 1H), 4.15-3.96 (m, 1H), 3.92 (s, 3H), 3.71-3.49 (m, 3H), 3.35- 3.17 (m, 2H), 3.07-2.97 (m, 2H), 2.88 (s, 3H), 2.47 (s, 3H), 2.33-1.87 (m, 10H).

[2176] Example 325. (2S)-1-[2-[4-[methyl-(8-methoxy-3-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2177] The title compound (53.3 mg, 14.8 %) was prepared in the same fashion as Example 121, except that 8-methoxy-N-methyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride (344.5 mg, 0.88 mmol) prepared in Reference Example 171 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline- 5 -amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.65 (d, 1H), 7.27 (t, 1H), 7.14 (d, 1H), 7.10 (d, 1H), 6.71 (d, 1H), 5.02-4.67 (m, 1H), 3.97 (s, 3H), 3.71-3.40 (m, 3H), 3.29-3.08 (m, 2H), 3.00- 2.90 (m, 2H), 2.83 (s, 3H), 2.31-1.97 (m, 6H), 1.92-1.82 (m, 2H), 1.71-1.68 (m, 2H).

[2178] Example 326. (2S,4S)-4-fluoro-1-[2-[4-[methyl-(6-methyl-3-quinolyl)amino] -1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2179] The title compound (13.0 mg, 28.9 %) was prepared in the same fashion as Example 131, except that N,6-dimethyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride (36.1 mg, 0.11 mmol) prepared in Reference Example 168 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.66 (d, 1H), 7.83 (d, 1H), 7.40 (s, 1H), 7.27- 7.24 (m, 1H), 7.12 (d, 1H), 5.49-4.93 (m, 2H), 4.19-4.01 (m, 1H), 3.81-3.70 (m, 2H), 3.29-3.15 (m, 2H), 3.09-2.93 (m,2H), 2.88 (s, 3H), 2.47 (s, 3H), 2.24-1.89 (m, 6H), 1.79-1.76 (m, 2H).

[2180] Example 327. (2S,4S)-4-fluoro-1-(2-(4-((6-methoxyquinolin-3- yl)(methyl)amino)piperidin- 1 -yl)acetyl)pyrrolidine-2-carbonitrile

[2181] The title compound (7.3 mg, 15.5 %) was prepared in the same fashion as Example 131, except that 6-methoxy-N-methyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride (37.8 mg, 0.11 mmol) prepared in Reference Example 169 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline- 5 -amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.5 4 (d, 1H), 7.80 (d, 1H), 7.09 (d, 1H), 7.04 (dd, 1H), 6.90 (d, 1H), 5.46-5.24 (m, 2H), 4.14-3.90 (m, 1H), 3.87 (s, 3H), 3.84-3.68 (m, 2H), 3.40- 3.13 (m, 2H), 3.07-2.92 (m, 2H), 2.86 (s, 3H), 2.75-2.57 (m, 1H), 2.37-2.20 (m, 3H), 2.10-1.84 (m, 2H), 1.76-1.63 (m, 2H).

[2182] Example 328. (2S,4S)-4-fluoro-1-(2-(4-((7-methoxyquinolin-3- yl)(methyl)amino)piperidin- 1 -yl)acetyl)pyrrolidine-2-carbonitrile

[2183] The title compound (11.6 mg, 24.7 %) was prepared in the same fashion as Example 131, except that 7-methoxy-N-methyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride (37.8 mg, 0.11 mmol) prepared in Reference Example 170 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline- 5 -amine hydrochloride. ¹ H NMR (CDC1 ₃ , 400 MHz) δ 8.68 (s, 1H), 7.55 (d, 1H), 7.32 (d, 1H), 7.24 (d, 1H), 7.13 (dd, 1H), 5.50-4.94 (m, 2H), 4.21-4.12 (m, 1H), 3.87 (s, 3H), 3.78-3.64 (m, 2H), 3.46- 3.17 (m, 2H), 3.10-2.97 (m, 2H), 2.88 (s, 3H), 2.80-2.64 (m, 1H), 2.49-2.24 (m, 3H), 2.12-2.02 (m, 1H), 1.97-1.84 (m, 3H).

[2184] Example 329. (2S,4S)-4-fluoro-1-[2-[4-[(8-methoxy-3-quinolyl)-methyl-amin o]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2185] The title compound (3.8 mg, 8.0 %) was prepared in the same fashion as Example 131, except that 8-methoxy-N-methyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride (37.8 mg, 0.11 mmol) prepared in Reference Example 171 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline- 5 -amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.72 (d, 1H), 7.35 (t, 1H), 7.22 (d, 1H), 7.18 (d, 1H), 6.80 (d, 1H), 5.50-4.94 (m, 2H), 4.21-4.12 (m, 1H), 4.06 (s, 3H), 3.88-3.73 (m, 2H), 3.46- 3.21 (m, 2H), 3.11-2.96 (m, 2H), 2.91 (s, 3H), 2.80-2.65 (m, 1H), 2.50-2.22 (m, 3H), 2.15-1.86 (m, 2H), 1.81-1.78 (m, 3H).

[2186] Example 330. (2S,4S)-4-fluoro-1-[2-[4-[(8-fluoro-5-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2187] (2S,4S)-1-(2-chloroacetyl)-4-fluoro-pyrrolidin-2-carbonitril e (90.4 mg, 0.47 mmol) prepared in Reference Example 2 was dissolved in anhydrous acetonitrile (4 ml). To the resulting solution, 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride (151.0 mg, 0.47 mmol) prepared in Reference Example 172 and potassium carbonate (262.3 mg, 1.90 mmol) were added. The reaction mixture was stirred overnight at room temperature. After the completion of the reaction, water was added to the reaction mixture. The aqueous layer of the reaction mixture was extracted with ethyl acetate. The extracted organic layer was dried and filtered over anhydrous magnesium sulfate. The filtered solution was concentrated and then purified with silica gel column chromatography (dichloromethane/methanol= 10/1, v/v) to give a title compound (76.2 mg, 40.2 %). ¹ H-NMR (CD ₃ OD, 400 MHz) δ 8.94 (d, 1H), 8.18 (d, 1H), 7.42 (d, 1H), 7.30-7.26 (m, 1H), 6.55 (d, 1H), 5.50- 5.30 (m, 1H), 5.10-4.80 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.85 (m, 1H), 3.85-3.70 (m, 2H), 3.55-3.15 (m, 2H), 3.05-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.30 (m, 2H), 2.20-2.05 (m, 2H), 1.90-1.55 (m, 3H)

[2188] Example 331. (2S,4S)-4-fluoro-1-[2-[4-[(8-methyl-5-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2189] The title compound (46.7 mg, 24.9 %) was prepared in the same fashion as Example 330, except that 8-methyl-N-(piperidin-4-yl)quinolin-5-amine hydrochloride (61.2 mg, 0.20 mmol) prepared in Reference Example 173 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.93 (d, 1H), 8.17 (d, 1H), 7.42-7.33 (m, 2H), 6.60 (d, 1H), 5.55-5.30 (m, 1H), 5.05-4.90 (m, 1H), 4.30-4.10 (m, 1H), 4.05-3.65 (m, 2H), 3.55-3.16 (m, 3H), 3.05-2.85 (m, 2H), 2.80-2.68 (m, 1H), 2.68 (s, 3H), 2.50-2.25 (m, 2H), 2.25-2.05 (m, 2H), 1.95-1.50 (m, 3H)

[2190] Example 332. (2S,4S)-1-[2-[4-[(8-benzyloxy-5-quinolyl)amino]-1-piperidyl] acetyl]-4- fluoro-pyrrolidine-2-carbonitrile

[2191] The title compound (109.7 mg, 47.4 %) was prepared in the same fashion as Example 330, except that 8-(benzyloxy)-N-(piperidin-4-yl)quinolin-5-amine hydrochloride (134.2 mg, 0.33 mmol) prepared in Reference Example 174 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.97 (d, 1H), 8.19 (d, 1H), 7.51-7.49 (m, 2H), 7.41-7.26 (m, 4H), 6.92 (d, 1H), 6,53 (d, 1H), 5.55-5.30 (m, 1H), 5.48 (s, 2H), 5.00-4.85 (m, 1H), 4.30-4.10 (m, 1H), 4.05-3.70 (m, 2H), 3.55-3.20 (m, 3H), 3.05-2.80 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.25 (m, 2H), 2.25-2.05 (m, 2H), 1.80-1.50 (m, 3H)

[2192] Example 333. (2S,4S)-1-[2-[4-[(3-chloro-5-quinolyl)amino]-1-piperidyl]ace tyl]-4-fluoro- pyrrolidine-2-carbonitrile

[2193] The title compound (191.7 mg, 97.1 %) was prepared in the same fashion as Example 330, except that 3-chloro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride (241.4 mg, 0.72 mmol) prepared in Reference Example 175 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.80 (s, 1H), 8.13 (s, 1H), 7.55 (t, 1H), 7.45 (d, 1H), 6.68 (d, 1H), 5.50-5.30 (m, 1H), 5.00-4.85 (m, 1H), 4.30-4.15 (m, 1H), 4.10-3.70 (m, 2H), 3.60-3.10 (m, 3H), 3.10-2.85 (m, 2H), 2.85-2.60 (m, 1H), 2.50-2.30 (m, 2H), 2.30-2.10 (m, 2H), 1.90-1.50 (m, 3H)

[2194] Example 334. (2S,4S)-4-fluoro-1-[2-[4-[[8-(trifluoromethyl)-5-quinolyl]am ino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2195] The title compound (116.6 mg, 54.7 %) was prepared in the same fashion as Example 330, except that N-(piperidin-4-yl)-8-(trifluoromethyl)quinolin-5-amine hydrochloride (137.5 mg, 0.37 mmol) prepared in Reference Example 176 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 9.01 (d, 1H), 8.16 (d, 1H), 7.89 (d, 1H), 7.41 (t, 1H), 6.56 (d, 1H), 5.55-5.30 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.10 (m, 1H), 4.05-3.70 (m, 2H), 3.70- 3.15 (m, 3H), 3.10-2.85 (m, 2H), 2.80-2.55 (m, 1H), 2.50-2.35 (m, 2H), 2.35-2.10 (m, 2H), 1.95-1.55 (m, 3H)

[2196] Example 335. 5-[[1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrrolidin-1-yl]eth yl]-4- piperidyl]amino]quinoline-2-carbonitrile

[2197] The title compound (42.1 mg, 21.8 %) was prepared in the same fashion as Example 330, except that 5-(piperidin-4-ylamino)quinoline-2-carbonitrile hydrochloride (299.7 mg, 0.92 mmol) prepared in Reference Example 177 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.29 (d, 1H), 7.68-7.59 (m, 2H), 7.50 (d, 1H), 6.76 (d, 1H), 5.60-5.30 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.10 (m, 1H), 4.10-3.70 (m, 2H), 3.60-3.20 (m, 3H), 3.10-2.90 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.30 (m, 2H), 2.30-2.10 (m, 2H), 1.90-1.55 (m, 3H)

[2198] Example 336. (2S,4S)-1-(2-(4-(benzo[b]thiophen-4-ylamino)piperidin-1-yl)a cetyl)-4- fluoropyrrolidine-2-carbonitrile

[2199] The title compound (239.9 mg, 93.6 %) was prepared in the same fashion as Example 330, except that N-(benzo[b]thiophen-4-yl)piperidin-4-amine hydrochloride (202.4 mg, 0.66 mmol) prepared in Reference Example 178 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.31 (s, 1H), 7.31-7.17 (m, 3H), 6.52 (d, 1H), 5.50-5.25 (m, 1H), 4.95-4.85 (m, 1H), 4.25-4.15 (m, 1H), 4.15-3.70 (m, 2H), 3.55-3.10 (m, 3H), 3.00-2.80 (m, 2H), 2.75-2.60 (m, 1H), 2.40-2.25 (m, 2H), 2.20-2.05 (m, 2H), 1.85-1.50 (m, 3H)

[2200] Example 337. (2S,4S)-4-fluoro-1-[2-[4-[(6-fluoro-4-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2201] The title compound (22.9 mg, 23.2 %) was prepared in the same fashion as Example 330, except that 6-fluoro-N-(piperidin-4-yl)quinolin-4-amine hydrochloride (154.5 mg, 0.49 mmol) prepared in Reference Example 179 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.53 (d, 1H), 7.97 (d, 1H), 7.40 (s, 1H), 7.33 (d, 1H), 6.46 (d, 1H), 5.55-5.35 (m, 1H), 5.05-4.95 (m, 1H), 4.30-4.10 (m, 1H), 3.90-3.50 (m, 3H), 3.50-3.10 (m, 2H), 3.05-2.90 (m, 2H), 2.85-2.70 (m, 1H), 2.50-2.35 (m, 2H), 2.25-2.10 (m, 2H), 1.80-1.60 (m, 3H)

[2202] Example 338. (2S,4S)-1-[2-[4-[(6-chloro-4-quinolyl)amino]-1-piperidyl]ace tyl]-4-fluoro- pyrrolidine-2-carbonitrile

[2203] The title compound (35.0 mg, 34.0 %) was prepared in the same fashion as Example 330, except that 6-chloro-N-(piperidin-4-yl)quinolin-4-amine hydrochloride (194.2 mg, 0.58 mmol) prepared in Reference Example 180 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.54 (d, 1H), 7.92 (d, 1H), 7.69 (s, 1H), 7.57 (d, 1H), 6.46 (d, 1H), 5.55-5.30 (m, 1H), 5.00-4.90 (m, 1H), 4.35-4.20 (m, 1H), 4.05-3.70 (m, 2H), 3.65-3.20 (m, 3H), 3.05-2.90 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.30 (m, 2H), 2.20-2.10 (m, 2H), 1.90-1.60 (m, 3H)

[2204] Example 339. (2S,4S)-4-fluoro-1-[2-[4-(4-isoquinolylamino)-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2205] The title compound (180.2 mg, 40.5 %) was prepared in the same fashion as Example 330, except that N-(piperidin-4-yl)isoquinolin-4-amine hydrochloride (350.0 mg, 1.17 mmol) prepared in

Reference Example 181 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.68 (s, 1H), 7.92-7.88 (m, 2H), 7.78 (d, 1H), 7.66 (t, 1H), 7.58 (t, 1H), 5.55-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.15 (m, 1H), 4.15-3.70 (m, 2H), 3.65- 3.20 (m, 3H), 3.10-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.30 (m, 2H), 2.30-2.15 (m, 2H), 1.90-1.65 (m, 3H)

[2206] Example 340. (2S,4S)-4-fluoro-1-[2-[4-(5-isoquinolylamino)-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2207] The title compound (153.2 mg, 34.5 %) was prepared in the same fashion as Example 330, except that N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride (305.9 mg, 1.02 mmol) prepared in

Reference Example 182 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 9.15 (s, 1H), 8.47 (d, 1H), 7.54 (d, 1H), 7.45 (t, 1H),

7.31 (d, 1H), 6.78 (d, 1H), 5.55-5.25 (m, 1H), 5.00-4.85 (m, 1H), 4.30-4.15 (m, 1H), 4.10-3.70 (m, 2H), 3.60-3.15 (m, 3H), 3.05-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.30 (m, 2H), 2.30-2.10 (m, 2H), 1.90-1.55 (m, 3H)

[2208] Example 341. (2S,4S)-4-fluoro-1-[2-[4-(6-isoquinolylamino)-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile [2209] The title compound (28.4 mg, 6.4 %) was prepared in the same fashion as Example 330, except that N-(piperidin-4-yl)isoquinolin-6-amine hydrochloride (137.6 mg, 0.46 mmol) prepared in

Reference Example 183 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.93 (s, 1H), 8.31 (d, 1H), 7.71 (d, 1H), 7.37 (d, 1H), 6.89 (d, 1H), 6.65 (s, 1H), 5.55-5.25 (m, 1H), 5.00-4.85 (m, 1H), 4.35-4.15 (m, 1H), 4.15-3.70 (m, 2H), 3.55-3.15 (m, 3H), 3.05-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.30 (m, 2H), 2.25-2.05 (m, 2H), 1.75-1.50 (m, 3H)

[2210] Example 342. (2S,4S)-4-fluoro-1-[2-[4-(8-isoquinolylamino)-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2211] The title compound (40.8 mg, 9.2 %) was prepared in the same fashion as Example 330, except that N-(piperidin-4-yl)isoquinolin-8-amine hydrochloride (374.8 mg, 1.25 mmol) prepared in

Reference Example 184 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 9.32 (s, 1H), 8.45 (d, 1H), 7.57-7.50 (m, 2H), 7.12 (d, 1H), 6.68 (d, 1H), 5.60-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.70 (m, 2H), 3.60- 3.20 (m, 3H), 3.05-2.85 (m, 2H), 2.85-2.60 (m, 1H), 2.55-2.30 (m, 2H), 2.30-2.10 (m, 2H), 1.90-1.60 (m, 3H)

[2212] Example 343. (2S,4S)-4-fluoro-1-[2-[4-[(8-methyl-4-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2213] The title compound (205.7 mg, 52.8 %) was prepared in the same fashion as Example 330, except that 8-methyl-N-(piperidin-4-yl)quinolin-4-amine hydrochloride (309.6 mg, 0.99 mmol) prepared in Reference Example 185 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.58 (d, 1H), 7.58 (d, 1H), 7.50 (d, 1H), 7.32 (t, 1H), 6.46 (d, 1H), 5.50-5.25 (m, 1H), 5.00-4.80 (m, 1H), 4.30-4.10 (m, 1H), 4.05-3.70 (m, 2H), 3.65-3.15 (m, 3H), 3.05-2.85 (m, 2H), 2.76 (s, 3H), 2.76-2.55 (m, 1H), 2.45-2.30 (m, 2H), 2.20-2.05 (m, 2H), 1.85-1.55 (m, 3H)

[2214] Example 344. (2S,4S)-1-[2-[4-[(8-chloro-4-quinolyl)amino]-1-piperidyl]ace tyl]-4-fluoro- pyrrolidine-2-carbonitrile

[2215] The title compound (16.6 mg, 4.1 %) was prepared in the same fashion as Example 330, except that 8-chloro-N-(piperidin-4-yl)quinolin-4-amine hydrochloride (70.1 mg, 0.21 mmol) prepared in Reference Example 186 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.54 (d, 1H), 7.46-7.37 (m, 3H), 6.71 (d, 1H), 5.70-5.20 (m, 1H), 5.05-4.85 (m, 1H), 4.30-4.10 (m, 1H), 4.05-3.70 (m, 2H), 3.60-3.15 (m, 3H), 3.00-2.80 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.30 (m, 2H), 2.20-2.05 (m, 2H), 1.90-1.50 (m, 3H) [2216] Example 345. (2S,4S)-4-fluoro-1-[2-[4-[(8-fluoro-4-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2217] The title compound (18.7 mg, 4.8 %) was prepared in the same fashion as Example 330, except that 8-fluoro-N-(piperidin-4-yl)quinolin-4-amine hydrochloride (192.9 mg, 0.61 mmol) prepared in Reference Example 187 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.59 (d, 1H), 7.49 (d, 1H), 7.36-7.33 (m, 2H), 6.50 (d, 1H), 5.55-5.25 (m, 1H), 5.05-4.90 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.70 (m, 2H), 3.65-3.20 (m, 3H), 3.10-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.30 (m, 2H), 2.20-2.05 (m, 2H), 1.90-1.50 (m, 3H)

[2218] Example 346. (2S,4S)-4-fluoro-1-[2-[4-[[8-(trifluoromethyl)-4-quinolyl]am ino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2219] The title compound (347.4 mg, 78.5 %) was prepared in the same fashion as Example 330, except that N-(piperidin-4-yl)-8-(trifluoromethyl)quinolin-4-amine hydrochloride (341.3 mg, 0.93 mmol) prepared in Reference Example 188 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.65 (d, 1H), 7.99-7.95 (m, 2H), 7.44 (t, 1H),

6.51 (d, 1H), 5.50-5.20 (m, 1H), 5.10-4.85 (m, 1H), 4.30-4.10 (m, 1H), 4.00-3.65 (m, 2H), 3.60-3.10 (m, 3H), 3.05-2.80 (m, 2H), 2.75-2.55 (m, 1H), 2.50-2.25 (m, 2H), 2.25-2.05 (m, 2H), 1.90-1.55 (m, 3H)

[2220] Example 347. (2S,4S)-4-fluoro-1-[2-[4-[[8-(trifluoromethoxy)-5-quinolyl]a mino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2221] The title compound (125.5 mg, 82.2 %) was prepared in the same fashion as Example 330, except that N-(piperidin-4-yl)-8-(trifluoromethoxy)quinolin-5-amine hydrochloride (408.2 mg, 1.06 mmol) prepared in Reference Example 189 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 9.00 (d, 1H), 8.18 (d, 1H), 7.50 (d, 1H), 7.43 (t, 1H), 6.56 (d, 1H), 5.55-5.25 (m, 1H), 5.05-4.90 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.70 (m, 2H), 3.60- 3.20 (m, 3H), 3.10-2.90 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.35 (m, 2H), 2.35-2.10 (m, 2H), 1.90-1.60 (m, 3H)

[2222] Example 348. (2S,4S)-4-fluoro-1-[2-[4-[(3-methoxy-5-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2223] The title compound (284.9 mg, 75.5 %) was prepared in the same fashion as Example 330, except that 3-methoxy-N-(piperidin-4-yl)quinolin-5-amine hydrochloride (302.7 mg, 0.92 mmol) prepared in Reference Example 190 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.65 (s, 1H), 7.47-7.38 (m, 2H), 7.34 (s, 1H), 6.68 (d, 1H), 5.50-5.20 (m, 1H), 5.00-4.85 (m, 1H), 4.30-4.15 (m, 1H), 3.90 (s, 3H), 4.10-3.70 (m, 2H), 3.55- 3.10 (m, 3H), 3.05-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.45-2.30 (m, 2H), 2.30-2.10 (m, 2H), 1.95-1.55

(m, 3H)

[2224] Example 349. (2S,4S)-4-fluoro-1-[2-[4-[(3-fluoro-5-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2225] The title compound (147.1 mg, 37.0 %) was prepared in the same fashion as Example 330, except that 3-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride (316.6 mg, 1.00 mmol) prepared in Reference Example 191 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.75 (s, 1H), 7.81 (d, 1H), 7.54-7.47 (m, 2H), 6.69 (d, 1H), 5.50-5.20 (m, 1H), 5.00-4.80 (m, 1H), 4.30-4.10 (m, 1H), 4.10-3.70 (m, 2H), 3.60-3.10 (m, 3H), 3.00-2.80 (m, 2H), 2.80-2.55 (m, 1H), 2.50-2.30 (m, 2H), 2.30-2.10 (m, 2H), 1.85-1.50 (m, 3H)

[2226] Example 350. (2S,4S)-4-fluoro-1-[2-[4-[furo[3,2-c]pyridin-7-yl(methyl)ami no]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2227] The title compound (8.9 mg, 21.0 %) was prepared in the same fashion as Example 131, except that N-methyl-N-(piperidin-4-yl)furo[3,2-c]pyridin-7-amine hydrochloride (33.4 mg, 0.11 mmol) prepared in Reference Example 161 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline- 5 -amine hydrochloride. ¹ H NMR (MeOD ₄ , 400 MHz) 8. δ35 (s, 1H), 7.87 (d, 2H), 6.96 (d, 1H), 5.51- 4.96 (m, 2H), 4.15-3.75 (m, 3H), 3.42-3.20 (m, 2H), 3.06-3.01 (m, 2H), 2.95 (s, 3H), 2.66-2.40 (m, 2H), 2.29-2.23 (m, 2H), 2.04-1.95 (m, 2H), 1.75-1.73 (m, 2H).

[2228] Example 351. (2S,4S)-1-[2-[4-[(1-chloro-5-isoquinolyl)amino]-1-piperidyl] acetyl]-4- fluoro-pyrrolidine-2-carbonitrile

[2229] The title compound (34.6 mg, 60.8 %) was prepared in the same fashion as Example 330, except that 1-chloro-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride (45.8 mg, 0.14 mmol) prepared in Reference Example 192 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.21 (d, 1H), 8.06 (d, 1H), 7.88-7.49 (m, 2H), 6.83 (d, 1H), 5.55-5.25 (m, 1H), 5.00-4.85 (m, 1H), 4.30-4.15 (m, 1H), 4.15-3.70 (m, 2H), 3.60-3.20 (m, 3H), 3.05-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.30 (m, 2H), 2.30-2.10 (m, 2H), 1.85-1.55 (m, 3H)

[2230] Example 352. (2S,4S)-1-[2-[4-[(3-chloro-5-isoquinolyl)amino]-1-piperidyl] acetyl]-4- fluoro-pyrrolidine-2-carbonitrile

[2231] The title compound (194.4 mg, 50.9 %) was prepared in the same fashion as Example 330, except that 3-chloro-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride (307.2 mg, 0.92 mmol) prepared in Reference Example 193 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.95 (s, 1H), 7.66 (s, 1H), 7.43 (t, 1H), 7.28 (d, 1H),

6.76 (d, 1H), 5.55-5.35 (m, 1H), 5.00-4.85 (m, 1H), 4.30-4.10 (m, 1H), 4.00-3.70 (m, 2H), 3.55-3.15 (m, 3H), 3.05-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.25 (m, 2H), 2.25-2.10 (m, 2H), 1.90-1.50 (m, 3H)

[2232] Example 353. (2S,4S)-1-[2-[4-[(8-chloro-5-isoquinolyl)amino]-1-piperidyl] acetyl]-4- fluoro-pyrrolidine-2-carbonitrile

[2233] The title compound (193.7 mg, 63.6 %) was prepared in the same fashion as Example 330, except that 8-chloro-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride (245.2 mg, 0.73 mmol) prepared in Reference Example 194 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 9.58 (s, 1H), 8.57 (d, 1H), 7.54 (d, 1H), 7.45 (d, 1H), 6.67 (d, 1H), 5.55-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.10 (m, 1H), 4.00-3.65 (m, 2H), 3.60-3.20 (m, 3H), 3.10-2.85 (m, 2H), 2.85-2.60 (m, 1H), 2.50-2.30 (m, 2H), 2.30-2.10 (m, 2H), 1.90-1.55 (m, 3H)

[2234] Example 354. (2S,4S)-4-fluoro-1-[2-[4-[(4-methyl-5-isoquinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2235] The title compound (307.2 mg, 93.3 %) was prepared in the same fashion as Example 330, except that 4-methyl-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride (261.6 mg, 0.83 mmol) prepared in Reference Example 195 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.93 (s, 1H), 8.11 (s, 1H), 7.39 (t, 1H), 7.27-7.23 (m, 1H), 6.73 (d, 1H), 5.50-5.20 (m, 1H), 5.00-4.80 (m, 1H), 4.30-4.10 (m, 1H), 4.05-3.70 (m, 2H), 3.55- 3.15 (m, 3H), 2.91 (s, 3H), 2.91-2.80 (m, 2H), 2.80-2.60 (m, 1H), 2.55-2.30 (m, 2H), 2.30-2.10 (m, 2H), 1.85-1.50 (m, 3H)

[2236] Example 355. (2S,4S)-4-fluoro-1-[2-[4-[(3-methoxy-5-isoquinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2237] The title compound (76.0 mg, 16.2 %) was prepared in the same fashion as Example 330, except that 3-methoxy-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride (376.0 mg, 1.14 mmol) prepared in Reference Example 196 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.86 (s, 1H), 7.48-7.21 (m, 2H), 6.91 (s, 1H), 6.66 (d, 1H), 5.60-5.30 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.10 (m, 1H), 4.05 (s, 3H), 4.05-3.70 (m, 2H), 3.60- 3.20 (m, 3H), 3.10-2.90 (m, 2H), 2.80-2.60 (m, 1H), 2.60-2.30 (m, 2H), 2.30-2.10 (m, 2H), 1.85-1.50 (m, 3H)

[2238] Example 356. (2S,4S)-4-fluoro-1-[2-[4-[(8-nitro-5-isoquinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2239] The title compound (95.6 mg, 19.7 %) was prepared in the same fashion as Example 330, except that 8-nitro-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride (165.1 mg, 0.48 mmol) prepared in Reference Example 197 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.66 (d, 1H), 8.50 (d, 1H), 7.58 (d, 1H), 7.26 (s, 1H), 6.66 (d, 1H), 5.60-5.20 (m, 1H), 5.00-4.85 (m, 1H), 4.30-4.10 (m, 1H), 4.00-3.70 (m, 2H), 3.70-3.15 (m, 3H), 3.10-2.90 (m, 2H), 2.85-2.60 (m, 1H), 2.55-2.30 (m, 2H), 2.30-2.10 (m, 2H), 1.85-1.50 (m, 3H)

[2240] Example 357. (2S,4S)-4-fluoro-1-[2-[4-[(8-fluoro-5-isoquinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2241] The title compound (225.9 mg, 49.7 %) was prepared in the same fashion as Example 330, except that 8-fluoro-N-(piperidin-4-yl)isoquinolin-5-amine hydrochloride (348.2 mg, 1.09 mmol) prepared in Reference Example 198 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 9.45 (s, 1H), 8.56 (d, 1H), 7.57 (d, 1H), 7.10 (d, 1H), 6.66 (d, 1H), 5.55-5.25 (m, 1H), 5.00-4.85 (m, 1H), 4.30-4.10 (m, 1H), 4.05-3.70 (m, 2H), 3.55-3.15 (m, 3H), 3.05-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.55-2.30 (m, 2H), 2.25-2.05 (m, 2H), 1.85-1.55 (m, 3H)

[2242] Example 358. (2S)-1-[2-[4-[(2-chloro-6,7-dimethoxy-quinazolin-4-yl)amino] -1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2243] The title compound (210.7 mg, 95.2 %) was prepared in the same fashion as Example 110, except that 2-chloro-6,7-dimethoxy-N-(piperidin-4-yl)quinazolin-4-amine hydrochloride (190.9 mg, 0.48 mmol) prepared in Reference Example 199 was used instead of N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.07-7.02 (m, 2H), 6.20-6.10 (m, 1H), 5.00-4.70 (m, 1H), 4.40-4.20 (m, 1H), 3.95 (s, 3H), 3.93 (s, 3H), 3.75-3.40 (m, 2H), 3.35-3.10 (m, 2H), 3.05- 2.85 (m, 2H), 2.45-2.25 (m, 3H), 2.25-2.00 (m, 4H), 1.90-1.60 (m, 3H)

[2244] Example 359. (2S)-1-[2-[4-[(2-chloro-8-methyl-quinazolin-4-yl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2245] The title compound (148.0 mg, 74.3 %) was prepared in the same fashion as Example 110, except that 2-chloro-8-methyl-N-(piperidin-4-yl)quinazolin-4-amine hydrochloride (128.7 mg, 0.37 mmol) prepared in Reference Example 200 was used instead of N-(piperidin-4-yl)quinoline-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.59-7.54 (m, 2H), 7.34 (t, 1H), 5.90-5.75 (m, 1H), 5.10-4.70 (m, 1H), 4.40-4.20 (m, 1H), 3.80-3.50 (m, 2H), 3.40-3.10 (m, 2H), 3.10-2.85 (m, 2H), 2.64 (s, 3H), 2.50-2.20 (m, 3H), 2.20-2.00 (m, 4H), 1.90-1.60 (m, 3H)

[2246] Example 360. (2S,4S)-4-fluoro-1-[2-[4-(2,6-naphthyridin-4-ylamino)-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2247] The title compound (55.6 mg, 42.1 %) was prepared in the same fashion as Example 330, except that N-(piperidin-4-yl)-2,6-naphthyridin-4-amine hydrochloride (104.1 mg, 0.35 mmol) prepared in Reference Example 201 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 9.10 (s, 1H), 8.58 (d, 1H), 8.11 (d, 1H), 7.54 (s, 1H), 7.00 (s, 1H), 5.55-5.25 (m, 1H), 5.05-4.90 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.70 (m, 2H), 3.50-3.15 (m, 3H), 3.05-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.30 (m, 2H), 2.30-2.10 (m, 2H), 1.95-1.60 (m, 3H)

[2248] Example 361. (2S,4S)-1-[2-[4-[(8-chloro-4-isoquinolyl)amino]-1-piperidyl] acetyl]-4- fluoro-pyrrolidine-2-carbonitrile

[2249] The title compound (91.6 mg, 63.7 %) was prepared in the same fashion as Example 330, except that 8-chloro-N-(piperidin-4-yl)isoquinolin-4-amine hydrochloride (95.1 mg, 0.28 mmol) prepared in Reference Example 202 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.29 (d, 1H), 7.81-7.66 (m, 3H), 7.61 (s, 1H), 5.55-5.30 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.15 (m, 1H), 4.15-3.70 (m, 2H), 3.60-3.20 (m, 3H), 3.10-2.90 (m, 2H), 2.85-2.65 (m, 1H), 2.50-2.35 (m, 2H), 2.35-2.15 (m, 2H), 1.90-1.60 (m, 3H)

[2250] Example 362. (2S,4S)-4-fluoro-1-[2-[4-(3-quinolylamino)-1-piperidyl]acety l]pyrrolidine-

2-carbonitrile

[2251] The title compound (154.9 mg, 60.1 %) was prepared in the same fashion as Example 330, except that N-(piperidin-4-yl)quinolin-3-amine hydrochloride (202.9 mg, 0.68 mmol) prepared in

Reference Example 203 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.44 (s, 1H), 7.95 (d, 1H), 7.62 (d, 1H), 7.44-7.43 (m, 2H), 7.03 (s, 1H), 5.50-5.30 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.15 (m, 1H), 4.15-3.70 (m, 2H), 3.55- 3.20 (m, 3H), 3.10-2.90 (m, 2H), 2.85-2.60 (m, 1H), 2.55-2.35 (m, 3H), 2.35-2.10 (m, 2H), 1.80-1.50 (m, 2H)

[2252] Example 363. (2S,4S)-4-fluoro-1-[2-[4-[(6-methyl-3-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2253] The title compound (102.5 mg, 38.4 %) was prepared in the same fashion as Example 330, except that 6-methyl-N-(piperidin-4-yl)quinolin-3-amine hydrochloride (131.9 mg, 0.42 mmol) prepared in Reference Example 204 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.35 (s, 1H), 7.82 (d, 1H), 7.38 (s, 1H), 7.24 (d, 1H), 6.95 (s, 1H), 5.55-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.10 (m, 1H), 4.05-3.70 (m, 2H), 3.55-3.20 (m, 3H), 3.05-2.80 (m, 2H), 2.80-2.60 (m, 1H), 2.45 (s, 3H), 2.45-2.20 (m, 3H), 2.20-2.10 (m, 2H), 1.80-1.50 (m, 2H)

[2254] Example 364. (2S,4S)-4-fluoro-1-[2-[4-[(6-fluoro-3-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2255] The title compound (167.9 mg, 62.2 %) was prepared in the same fashion as Example 330, except that 6-fluoro-N-(piperidin-4-yl)quinolin-3-amine hydrochloride (201.1 mg, 0.63 mmol) prepared in Reference Example 205 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.35 (s, 1H), 7.90 (d, 1H), 7.23-7.13 (m, 2H), 6.93 (s, 1H), 5.55-5.30 (m, 1H), 5.05-4.95 (m, 1H), 4.30-4.10 (m, 1H), 4.05-3.75 (m, 2H), 3.55-3.20 (m, 3H), 3.10-2.90 (m, 2H), 2.85-2.60 (m, 1H), 2.50-2.25 (m, 3H), 2.25-2.10 (m, 2H), 1.85-1.50 (m, 2H)

[2256] Example 365. (2S,4S)-4-fluoro-1-[2-[4-[(6-methoxy-3-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2257] The title compound (85.7 mg, 30.8 %) was prepared in the same fashion as Example 330, except that 6-methoxy-N-(piperidin-4-yl)quinolin-3-amine hydrochloride (179.4 mg, 0.54 mmol) prepared in Reference Example 206 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.26 (s, 1H), 7.82 (d, 1H), 7.06 (d, 1H), 6.93 (s, 1H), 6.91 (s, 1H), 5.50-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.10 (m, 1H), 3.90 (s, 3H), 4.05-3.70 (m, 2H), 3.55-3.20 (m, 3H), 3.05-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.30 (m, 3H), 2.30-2.10 (m, 2H), 1.85-1.50 (m, 2H)

[2258] Example 366. (2S,4S)-4-fluoro-1-[2-[4-[(7-methoxy-3-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2259] The title compound (39.6 mg, 14.2 %) was prepared in the same fashion as Example 330, except that 7-methoxy-N-(piperidin-4-yl)quinolin-3-amine hydrochloride (61.0 mg, 0.19 mmol) prepared in Reference Example 207 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.38 (s, 1H), 7.52 (d, 1H), 7.31 (s, 1H), 7.13 (d, 1H), 7.05 (s, 1H), 5.55-5.30 (m, 1H), 5.00-4.90 (m, 1H), 4.25-4.10 (m, 1H), 3.90 (s, 3H), 4.05-3.70 (m, 2H), 3.55-3.20 (m, 3H), 3.05-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.25 (m, 3H), 2.25-2.05 (m, 2H), 1.80-1.50 (m, 2H)

[2260] Example 367. (2S,4S)-4-fluoro-1-[2-[4-[(8-methoxy-3-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2261] The title compound (29.5 mg, 10.6 %) was prepared in the same fashion as Example 330, except that 8-methoxy-N-(piperidin-4-yl)quinolin-3-amine hydrochloride (72.5 mg, 0.22 mmol) prepared in Reference Example 208 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.42 (s, 1H), 7.36 (t, 1H), 7.19 (d, 1H), 7.00 (s, 1H),

6.79 (d, 1H), 5.55-5.30 (m, 1H), 5.05-4.90 (m, 1H), 4.30-4.10 (m, 1H), 4.06 (s, 3H), 4.06-3.70 (m, 2H), 3.60-3.25 (m, 3H), 3.10-2.90 (m, 2H), 2.85-2.60 (m, 1H), 2.60-2.35 (m, 3H), 2.35-2.10 (m, 2H), 1.80-1.55 (m, 2H)

[2262] Example 368. (2S,4S)-4-fluoro-1-[2-[4-(1,8-naphthyridin-3-ylamino)-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2263] The title compound (109.4 mg, 49.7 %) was prepared in the same fashion as Example 330, except that N-(piperidin-4-yl)-1,8-naphthyridin-3-amine hydrochloride (173.4 mg, 0.58 mmol) prepared in Reference Example 209 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 600MHz) δ 8.79 (d, 1H), 8.59 (s, 1H), 7.96 (d, 1H), 7.35 (t, 1H),

6.95 (s, 1H), 5.50-5.30 (m, 1H), 5.00-4.90 (m, 1H), 4.25-4.15 (m, 1H), 4.00-3.70 (m, 2H), 3.50-3.20 (m, 3H), 3.05-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.30 (m, 3H), 2.20-2.10 (m, 2H), 1.80-1.55 (m, 2H)

[2264] Example 369. (2S,4S)-1-[2-[4-[(7-chloro-1,8-naphthyridin-3-yl)amino]-1- piperidyl]acetyl]-4-fluoro-pyrrolidine-2-carbonitrile

[2265] The title compound (13.8 mg, 20.6 %) was prepared in the same fashion as Example 330, except that 7-chloro-N-(piperidin-4-yl)-1,8-naphthyridin-3-amine hydrochloride (53.6 mg, 0.16 mmol) prepared in Reference Example 210 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 600MHz) δ 8.55 (s, 1H), 7.90 (d, 1H), 7.35 (d, 1H), 6.95 (s, 1H), 5.50-5.30 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.10 (m, 1H), 4.00-3.70 (m, 2H), 3.50-3.20 (m, 3H), 3.05-2.90 (m, 2H), 2.80-2.65 (m, 1H), 2.50-2.30 (m, 3H), 2.20-2.10 (m, 2H), 1.80-1.55 (m, 2H)

[2266] Example 370. (2S)-1-[2-[4-[(8-ethoxy-5-quinolyl)amino]-1-piperidyl]acetyl ]pyrrolidine-

2-carbonitrile

[2267] The title compound (35.4 mg, 9.8 %) was prepared in the same fashion as Example 121, except that 8-ethoxy-N-(piperidin-4-yl)quinolin-5-amine hydrochloride (302.9 mg, 0.88 mmol) prepared in Reference Example 211 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. 1H NMR (CDC13, 400 MHz) δ 8.90 (dd, 1H), 8.18 (dd, 1H), 7.34 (q, 1H), 6.93 (d, 1H), 6.59 (d, 1H), 5.17-4.69 (m, 1H), 4.21 (q, 2H), 3.73-3.58 (m, 2H), 3.53-3.46 (m, 1H), 3.37- 3.13 (m, 3H), 2.93-2.83 (m, 2H), 2.35-2.00 (m, 8H), 1.73-1.56 (m, 2H), 1.53 (t, 3H).

[2268] Example 371. (2S,4S)-1-[2-[4-[(8-ethoxy-5-quinolyl)amino]-1-piperidyl]ace tyl]-4-fluoro- pyrrolidine-2-carbonitrile

[2269] The title compound (2.4 mg, 5.0 %) was prepared in the same fashion as Example 131, except that 8-ethoxy -N-(piperidin-4-yl)quinolin-5-amine hydrochloride (37.8 mg, 0.11 mmol) prepared in Reference Example 211 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. 1H NMR (CDC13, 400 MHz) δ 8.90 (dd, 1H), 8.18 (dd, 1H), 7.36 (q, 1H), 6.94 (d, 1H), 6.60 (d, 1H), 5.46-4.87 (m, 2H), 4.22 (q, 2H), 4.17-3.67 (m, 1H), 3.83-3.68 (m, 2H), 3.44- 3.21 (m, 2H), 3.18 (s, 1H), 2.96-2.84 (m, 2H), 2.73-2.56 (m, 1H), 2.35-2.10 (m, 5H), 1.75-1.53 (m, 5H).

[2270] Example 372. (2S,4S)-1-[2-[4-(1,3-benzothiazol-7-ylamino)-1-piperidyl]ace tyl]-4-fluoro- pyrrolidine-2-carbonitrile

[2271] The title compound (266.8 mg, 61.2 %) was prepared in the same fashion as Example 330, except that N-(piperidin-4-yl)benzo[d]thiazol-7-amine hydrochloride (344.8 mg, 1.13 mmol) prepared in Reference Example 212 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 600MHz) δ 8.92 (s, 1H), 7.56 (d, 1H), 7.38 (t, 1H), 6.66 (d, 1H), 5.50-5.30 (m, 1H), 5.00-4.90 (m, 1H), 4.25-4.15 (m, 1H), 4.00-3.70 (m, 2H), 3.60-3.15 (m, 3H), 3.00- 2.80 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.20 (m, 3H), 2.20-2.10 (m, 2H), 1.85-1.50 (m, 2H)

[2272] Example 373. (2S,4S)-1-[2-[4-[(l-acetylindolin-4-yl)amino]-1-piperidyl]ac etyl]-4-fluoro- pyrrolidine-2-carbonitrile

[2273] The title compound (45.8 mg, 12.8 %) was prepared in the same fashion as Example 330, except that 1-(4-(piperidin-4-ylamino)indolin-1-yl)ethan-1-one hydrochloride (286.8 mg, 0.86 mmol) prepared in Reference Example 213 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 7.63 (d, 1H), 7.10 (t, 1H), 6.36 (d, 1H), 5.50-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.10 (m, 1H), 4.09 (t, 2H), 4.00-3.70 (m, 2H), 3.50-3.15 (m, 3H), 3.00- 2.80 (m, 4H), 2.80-2.60 (m, 1H), 2.45-2.25 (m, 3H), 2.21 (s, 3H), 2.20-2.00 (m, 2H), 1.75-1.45 (m, 2H)

[2274] Example 374. (2S,4S)-4-fluoro-1-[2-[4-[(8-methyl-6-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2275] The title compound (149.1 mg, 65.6 %) was prepared in the same fashion as Example 330, except that 8-methyl-N-(piperidin-4-yl)quinolin-6-amine hydrochloride (180.7 mg, 0.58 mmol) prepared in Reference Example 214 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.64 (d, 1H), 7.88 (d, 1H), 7.28-7.24 (m, 1H), 6.94 (s, 1H), 6.57 (s, 1H), 5.50-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.70 (m, 2H), 3.50- 3.20 (m, 3H), 3.00-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.72 (s, 3H), 2.50-2.20 (m, 3H), 2.20-2.10 (m, 2H), 1.80-1.50 (m, 2H)

[2276] Example 375. (2S,4S)-4-fluoro-1-[2-[4-[(8-fluoro-6-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2277] The title compound (305.3 mg, 83.1 %) was prepared in the same fashion as Example 330, except that 8-fluoro-N-(piperidin-4-yl)quinolin-6-amine hydrochloride (292.4 mg, 0.92 mmol) prepared in Reference Example 215 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.63 (s, 1H), 7.89 (d, 1H), 7.32-7.28 (m, 1H), 6.80 (d, 1H), 6.50 (s, 1H), 5.50-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.70 (m, 2H), 3.50- 3.20 (m, 3H), 3.05-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.20 (m, 3H), 2.20-2.05 (m, 2H), 1.80-1.50 (m, 2H)

[2278] Example 376. (2S,4S)-1-[2-[4-[(8-chloro-6-quinolyl)amino]-1-piperidyl]ace tyl]-4-fluoro- pyrrolidine-2-carbonitrile

[2279] The title compound (265.9 mg, 62.6 %) was prepared in the same fashion as Example 330, except that 8-chloro-N-(piperidin-4-yl)quinolin-6-amine hydrochloride (341.7 mg, 1.02 mmol) prepared in Reference Example 216 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.80 (d, 1H), 8.71 (d, 1H), 7.32 (t, 1H), 7.24 (s, 1H),

6.62 (s, 1H), 5.50-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.10 (m, 1H), 4.05-3.70 (m, 2H), 3.50-3.20 (m, 3H), 3.00-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.20 (m, 3H), 2.20-2.05 (m, 2H), 1.80-1.50 (m, 2H)

[2280] Example 377. (2S,4S)-4-fluoro-1-[2-[4-[[8-(trifluoromethyl)-6-quinolyl]am ino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2281] The title compound (270.6 mg, 83.7 %) was prepared in the same fashion as Example 330, except that N-(piperidin-4-yl)-8-(trifluoromethyl)quinolin-6-amine hydrochloride (264.8 mg, 0.72 mmol) prepared in Reference Example 217 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.75 (d, 1H), 7.95 (d, 1H), 7.44 (s, 1H), 7.35 (t, 1H), 6.83 (s, 1H), 5.50-5.30 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.10 (m, 1H), 4.05-3.70 (m, 2H), 3.55- 3.20 (m, 3H), 3.05-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.20 (m, 3H), 2.20-2.05 (m, 2H), 1.80-1.50 (m, 2H)

[2282] Example 378. (2S)-1-[2-[4-[(2-oxochromen-4-yl)amino]-1-piperidyl]acetyl]p yrrolidine-2- carbonitrile

[2283] The title compound (25.9 mg, 72.0 %) was prepared in the same fashion as Example 121, except that 4-(piperidin-4-ylamino)-2H-chromen-2-one hydrochloride (30.0 mg, 0.09 mmol) prepared in Reference Example 218 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 7.55-7.47 (m, 2H), 7.33 (d, 1H), 7.28-7.24 (m, 1H),

5.34 (d, 1H), 5.22-5.15 (m, 1H), 5.03-4.75 (m, 1H), 3.75-3.63 (m, 1H), 3.60-3.42 (m, 2H), 3.32-3.17 (m, 2H), 3.04-2.91 (m, 2H), 2.39-2.06 (m, 8H), 1.92-1.66 (m, 2H).

[2284] Example 379. (2S,4S)-4-fluoro-1-[2-[4-[(2-oxochromen-4-yl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2285] The title compound (20.3 mg, 53.8 %) was prepared in the same fashion as Example 131, except that 4-(piperidin-4-ylamino)-2H-chromen-2-one hydrochloride (30.0 mg, 0.09 mmol) prepared in Reference Example 218 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 7. 47 (m, 2H), 7.33 (d, 1H), 7.28-7.24 (m, 1H), 5.50-5.25 (m, 2.4H), 5.20-5.14 (m, 1H), 4.95 (d, 0.6H), 4.22-4.16 (m, 1.5H), 4.00-3.72 (m, 1.5H), 3.49-3.41 (m, 1H), 3.37-3.17 (m, 2H), 3.03-2.92 (m, 2H), 2.79-2.64 (m, 1H), 2.41-2.31 (m, 3H), 2.17- 2.07 (m, 2H), 1.94-1.64 (m, 2H).

[2286] Example 380. (2S,4S)-4-fluoro-1-[2-[4-(1,5-naphthyridin-4-ylamino)-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2287] The title compound (4.3 mg, 10.2 %) was prepared in the same fashion as Example 131, except that N-(piperidin-4-yl)-1,5-naphthyridin-4-amine hydrochloride (33.1 mg, 0.11 mmol) prepared in Reference Example 219 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDC1 ₃ , 400 MHz) δ 8.69 (dd, 1H), 8.54 (d, 1H), 8.22 (dd, 1H), 7.57 (q, 1H), 6.65-6.62 (m, 1H), 6.53 (d, 1H), 5.51-4.94 (m, 2H), 4.26-3.93 (m, 1H), 3.90-3.73 (m, 1H), 3.59-3.26 (m, 2H), 3.25 (s, 1H), 3.03-2.90 (m, 2H), 2.80-2.64 (m, 1H), 2.49-2.16 (m, 4H), 1.89-1.67 (m, 3H).

[2288] Example 381. (2S,4S)-4-fluoro-1-[2-[4-[[2-(trifluoromethyl)-6-quinolyl]am ino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2289] The title compound (429.7 mg, 81.7 %) was prepared in the same fashion as Example 330, except that N-(piperidin-4-yl)-2-(trifluoromethyl)quinolin-6-amine hydrochloride (430.8 mg, 1.17 mmol) prepared in Reference Example 220 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.02 (d, 1H), 7.95 (d, 1H), 7.57 (d, 1H), 7.13 (d, 1H), 6.70 (s, 1H), 5.50-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.10 (m, 1H), 4.05-3.70 (m, 2H), 3.55- 3.20 (m, 3H), 3.05-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.20 (m, 3H), 2.20-2.10 (m, 2H), 1.85-1.55 (m, 2H)

[2290] Example 382. (2S,4S)-4-fluoro-1-[2-[4-[(4-methoxy-6-quinolyl)amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2291] The title compound (25.9 mg, 20.5 %) was prepared in the same fashion as Example 330, except that 4-methoxy-N-(piperidin-4-yl)quinolin-6-amine hydrochloride (101.7 mg, 0.31 mmol) prepared in Reference Example 221 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.49 (d, 1H), 7.83 (d, 1H), 7.08-7.03 (m, 2H), 6.66 (d, 1H), 5.50-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.15 (m, 1H), 4.03 (s, 3H), 4.00-3.70 (m, 2H), 3.55- 3.20 (m, 3H), 3.05-2.85 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.25 (m, 3H), 2.20-2.10 (m, 2H), 1.85-1.50 (m, 2H) [2292] Example 383. (2S,4S)-1-[2-[4-[(2-bromo-4-pyridyl)amino]-1-piperidyl]acety l]-4-fluoro- pyrrolidine-2-carbonitrile

[2293] The title compound (65.4 mg, 22.1 %) was prepared in the same fashion as Example 330, except that 2-bromo-N-(piperidin-4-yl)pyridin-4-amine hydrochloride (237.3 mg, 0.72 mmol) prepared in Reference Example 222 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 7.91 (d, 1H), 6.59 (s, 1H), 6.36 (d, 1H), 5.50-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.25-4.10 (m, 1H), 4.05-3.70 (m, 2H), 3.45-3.15 (m, 3H), 3.05-2.80 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.25 (m, 3H), 2.05-2.00 (m, 2H), 1.80-1.50 (m, 2H)

[2294] Example 384. (2S,4S)-1-[2-[4-(3H-benzimidazol-4-ylamino)-1-piperidyl]acet yl]-4-fluoro- pyrrolidine-2-carbonitrile

[2295] The title compound (363.5 mg, 97.2 %) was prepared in the same fashion as Example 330, except that N-(piperidin-4-yl)-1H-benzo[d]imidazol-7-amine hydrochloride (292.1 mg, 1.01 mmol) prepared in Reference Example 223 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (MeOD, 400MHz) δ 7.99 (s, 1H), 7.08 (t, 1H), 6.85 (d, 1H), 6.45 (d, 1H), 5.55-5.30 (m, 1H), 5.00-4.90 (m, 1H), 4.20-4.05 (m, 1H), 3.95-3.70 (m, 2H), 3.60-3.20 (m, 3H), 3.00- 2.90 (m, 2H), 2.70-2.55 (m, 1H), 2.55-2.30 (m, 3H), 2.20-2.05 (m, 2H), 1.85-1.60 (m, 2H)

[2296] Example 385. (2S,4S)-1-[2-[4-[(2-chloro-6-morpholino-4-pyridyl)amino]-1- piperidyl]acetyl]-4-fluoro-pyrrolidine-2-carbonitrile

[2297] The title compound (157.9 mg, 29.9 %) was prepared in the same fashion as Example 330, except that 2-chloro-6-morpholino-N-(piperidin-4-yl)pyridin-4-amine hydrochloride (431.5 mg, 1.17 mmol) prepared in Reference Example 224 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 5.95 (s, 1H), 5.56 (s, 1H), 5.50-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.25-4.05 (m, 1H), 4.00-3.70 (m, 6H), 3.50-3.40 (m, 4H), 3.40-3.15 (m, 3H), 3.00- 2.80 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.20 (m, 3H), 2.10-1.95 (m, 2H), 1.70-1.45 (m, 2H)

[2298] Example 386. (2S,4S)-4-fluoro-1-[2-[4-[[3-(trifluoromethyl)-5-quinolyl]am ino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2299] The title compound (450.5 mg, 92.9 %) was prepared in the same fashion as Example 330, except that N-(piperidin-4-yl)-3-(trifluoromethyl)quinolin-5-amine hydrochloride (397.5 mg, 1.08 mmol) prepared in Reference Example 225 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5- amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 9.03 (s, 1H), 8.43 (s, 1H), 7.67 (t, 1H), 7.50 (d, 1H), 6.73 (d, 1H), 5.55-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.70 (m, 2H), 3.60- 3.20 (m, 3H), 3.10-2.90 (m, 2H), 2.80-2.60 (m, 1H), 2.50-2.25 (m, 3H), 2.25-2.10 (m, 2H), 1.90-1.60 (m, 2H) [2300] Example 387. (2S)-1-[2-[4-[[3-(trifluoromethyl)-5-quinolyl]amino]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2301] The title compound (324.7 mg, 69.7 %) was prepared in the same fashion as Example 110, except that N-(piperidin-4-yl)-3-(trifluoromethyl)quinolin-5-amine hydrochloride (397.5 mg, 1.08 mmol) prepared in Reference Example 225 was used instead of N-(piperidin-4-yl)quinoline-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 9.03 (s, 1H), 8.42 (s, 1H), 7.67 (t, 1H), 7.51 (d, 1H),

6.73 (d, 1H), 5.20-4.70 (m, 1H), 4.50-4.35 (m, 1H), 3.85-3.60 (m, 1H), 3.60-3.45 (m, 3H), 3.45-3.20 (m, 2H), 3.10-2.90 (m, 2H), 2.50-2.35 (m, 2H), 2.35-2.10 (m, 5H), 1.90-1.60 (m, 2H)

[2302] Example 388. 5-[[1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrrolidin-1-yl]eth yl]-4- piperidyl]amino]-N-phenyl-quinoline-3-carboxamide

[2303] The title compound (29.3 mg, 53.3 %) was prepared in the same fashion as Example 131, except that N-phenyl-5-(piperidin-4-ylamino)quinoline-3-carboxamide hydrochloride (46.0 mg, 0.11 mmol) prepared in Reference Example 226 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline- 5 -amine hydrochloride. 1HNMR (CDC13, 400MHz) δ 9.33 (d, 1H), 8.91 (d, 1H), 8.69 (d, 1H), 7.72- 7.70 (m, 2H), 7.64-7.59 (m, 1H), 7.46-7.43 (m, 1H), 7.38 (q, 2H), 7.18 (q, 1H), 6.67-6.62 (m, 1H), 5.48-4.92 (m, 2H), 4.03-3.70 (m, 2H), 3.47-3.23 (m, 2H), 3.19 (d, 1H), 3.00-2.61 (m, 3H), 2.40-2.28 (m, 3H), 2.17-2.01 (m, 2H), 1.65-1.46 (m, 3H).

[2304] Example 389. N-benzyl-N-methyl-5-[[1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-p yrrolidin-1- yl]ethyl]-4-piperidyl]amino]quinoline-3-carboxamide

[2305] The title compound (42.1 mg, 72.4 %) was prepared in the same fashion as Example 131, except that N-benzyl-N-methyl-5-(piperidin-4-ylamino)quinoline-3-carboxa mide hydrochloride (50.2 mg, 0.11 mmol) prepared in Reference Example 227 was used instead of 3-methyl-N-(piperidin-4- yl)quinoline-5 -amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.90 (s, 1H), 8.37 (d, 1H), 7.55 (s, 1H), 7.36-7.19 (m, 6H), 6.60 (s, 1H), 5.45-4.89 (m, 2H), 4.76-3.73 (m, 5H), 3.44-2.88 (m, 8H), 2.73- 2.60 (m, 1H), 2.47-2.22 (m, 3H), 2.09-2.01 (m, 2H), 1.75-1.48 (m, 2H).

[2306] Example 390. N-methyl-5-[[1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrrolidin -1-yl]ethyl]-

4-piperidyl]amino]quinoline-8-carboxamide

[2307] The title compound (11.7 mg, 24.4 %) was prepared in the same fashion as Example 131, except that N-methyl-5-(piperidin-4-ylamino)quinoline-8-carboxamide hydrochloride (39.2 mg, 0.11 mmol) prepared in Reference Example 228 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-

5 -amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 10.92 (d, 1H), 8.83 (dd, 1H), 8.68 (d, 1H), 8.26 (d, 1H), 7.36-7.33 (m, 1H), 6.65 (dd, 1H), 5.47-4.91 (m, 2H), 4.89-4.85 (m, 1H), 4.21-3.71 (m, 2H), 3.60-3.50 (m, 1H), 3.42-3.16 (m, 3H), 3.09 (d, 3H), 3.00-2.89 (m, 2H), 2.74-2.60 (m, 1H), 2.40-1.58 (m, 6H).

[2308] Example 391. N,N-dimethyl-5-[[1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrrol idin-1- yl]ethyl]-4-piperidyl]amino]quinoline-8-carboxamide

[2309] The title compound (24.2 mg, 48.7 %) was prepared in the same fashion as Example 131, except that N,N-dimethyl-5-(piperidin-4-ylamino)quinoline-8-carboxamide hydrochloride (40.7 mg, 0.11 mmol) prepared in Reference Example 229 was used instead of 3-methyl-N-(piperidin-4- yl)quinoline-5 -amine hydrochloride. ¹ H NMR (CDCI ₃ , 400MHz) δ 8.82 (dd, 1H), 8.16 (d, 1H), 7.45 (d, 1H), 7.27-7.23 (m, 1H), 6.54 (d, 1H), 5.44-4.86 (m, 2H), 4.63 (d, 1H), 4.19-3.71 (m, 2H), 3.46- 3.47 (m, 3H), 3.28-3.13 (m, 4H), 2.91-2.86 (m, 2H), 2.78 (s, 3H), 2.70-2.54 (m, 1H), 2.74-2.60 (m, 1H), 2.46-2.02 (m, 5H).

[2310] Example 392. N-methyl-5-[[1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrrolidin -1-yl]ethyl]-

4-piperidyl]amino]-N-phenyl-quinoline-8-carboxamide

[2311] The title compound (11.4 mg, 20.3 %) was prepared in the same fashion as Example 131, except that N-methyl-N-phenyl-5-(piperidin-4-ylamino)quinoline-8-carboxa mide hydrochloride (47.5 mg, 0.11 mmol) prepared in Reference Example 230 was used instead of 3-methyl-N-(piperidin-4- yl)quinoline-5 -amine hydrochloride. ¹ H NMR (CDCI ₃ , 400MHz) δ 8.84-8.83 (m, 1H), 8.04-8.02 (d, 1H), 7.27-6.99 (m, 7H), 6.34 (s, 1H), 5.41-5.20 (m, 2H), 4.48 (s, 1H), 4.14-3.82 (m, 1H), 3.80-3.65 (m, 1H), 3.63-3.41 (m, 3H), 3.37-3.21 (m, 2H), 3.17-3.10 (m, 1H), 2.91-2.79 (m, 2H), 2.66-2.51 (m, 1H), 2.36-2.00 (m, 5H), 1.74-1.51 (m, 2H).

[2312] Example 393. N-benzyl-N-methyl-5-[[1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-p yrrolidin-1- yl]ethyl]-4-piperidyl]amino]quinoline-8-carboxamide

[2313] The title compound (12.5 mg, 21.5 %) was prepared in the same fashion as Example 131, except that N-benzyl-N-methyl-5-(piperidin-4-ylamino)quinoline-8-carboxa mide hydrochloride (49.1 mg, 0.11 mmol) prepared in Reference Example 231 was used instead of 3-methyl-N-(piperidin-4- yl)quinoline-5 -amine hydrochloride. ¹ H NMR (CDCT,. 400 MHz) δ 8.81 (s, 1H), 8.22-8.16 (m, 1H), 7.49 (t, 2H), 7.34 (t, 1H), 7.27-7.14 (m, 4H), 6.50 (q, 1H), 5.39-4.89 (m, 2H), 4.30 (q, 1H), 4.08- 4.01 (m, 1H), 3.88-3.61 (m, 2H), 3.40-3.20 (m, 2H), 3.16-3.05 (m, 3H), 2.83-2.82 (m, 2H), 2.65-2.45 (m, 3H), 2.39-2.00 (m, 5H), 1.77-1.58 (m, 2H).

[2314] Example 394. 5-[[1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrrolidin-1-yl]eth yl]-4- piperidyl]amino]-N-phenyl-quinoline-8-carboxamide

[2315] The title compound (29.6 mg, 53.7 %) was prepared in the same fashion as Example 131, except that N-phenyl-5-(piperidin-4-ylamino)quinoline-8-carboxamide hydrochloride (54.0 mg, 0.11 mmol) prepared in Reference Example 232 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline- 5-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 13.47 (s, 1H), 8.87-8.85 (m, 1H), 8.71 (dd,

1H), 8.28 (dd, 1H), 7.83 (d, 2H), 7.38-7.32 (m, 3H), 7.09 (t, 1H), 6.64 (t, 1H), 5.42-4.88 (m, 3H),

4.14-3.86 (m, 1H), 3.84-3.65 (m, 1H), 3.58-3.47 (m, 1H), 3.34-3.12 (m, 2H), 2.99-2.87 (m, 2H), 2.69-

2.56 (m, 1H), 2.43-2.10 (m, 5H), 1.90-1.63 (m, 2H).

[2316] Example 395. N-methyl-5-[[1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1-yl]ethyl]- 4- piperidyl]amino]quinoline-8-carboxamide

[2317] The title compound (243.0 mg, 65.6 %) was prepared in the same fashion as Example 121, except that N-methyl-5-(piperidin-4-ylamino)quinoline-8-carboxamide hydrochloride (313.6 mg, 0.88 mmol) prepared in Reference Example 228 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline- 5 -amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 10.91 (d, 1H), 8.84 (dd, 1H), 8.70 (d, 1H), 8.25 (d, 1H), 7.37-7.34 (m, 1H), 6.67 (d, 1H), 5.10-4.74 (m, 2H), 3.76-3.49 (m, 3H), 3.37-3.17 (m, 2H), 3.09 (d, 3H), 3.01-2.89 (m, 2H), 2.42-2.07 (m, 8H), 1.88-1.63 (m, 2H)

[2318] Example 396. 5-[[1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1-yl]ethyl]-4-piperid yl]amino]-N- phenyl-quinoline-8-carboxamide

[2319] The title compound (185.5 mg, 43.6 %) was prepared in the same fashion as Example 121, except that N-phenyl-5-(piperidin-4-ylamino)quinoline-8-carboxamide hydrochloride (369.0 mg, 0.88 mmol) prepared in Reference Example 232 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline- 5 -amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 13.466s, 1H), 8.89 (d, 1H), 8.75 (d, 1H), 8.27 (d, 1H), 7.85 (d, 2H), 7.39-7.35 (m, 3H), 7.09 (t, 1H), 6.70-6.66 (m, 1H), 5.08-4.74 (m, 2H), 3.73-3.47 (m, 3H), 3.36-3.16 (m, 2H), 2.99-2.89 (m, 2H), 2.41-2.13 (m, 8H), 1.90-1.64 (m, 2H)

[2320] Example 397. N-benzyl-N-methyl-5-[[1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1-y l]ethyl]-4- piperidyl]amino]quinoline-8-carboxamide

[2321] The title compound (256.2 mg, 57.0 %) was prepared in the same fashion as Example 121, except that N-benzyl-N-methyl-5-(piperidin-4-ylamino)quinoline-8-carboxa mide hydrochloride (393.7 mg, 0.88 mmol) prepared in Reference Example 231 was used instead of 3-methyl-N- (piperidin-4-yl)quinoline-5-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.89-8.86 (m, 1H), 8.17-8.12 (m, 1H), 7.53 (d, 2H), 7.38 (t, 1H), 7.30-7.18 (m, 4H), 6.56 (dd, 1H), 5.17-4.73 (m, 2H),

4.56 (s, 1H), 4.34 (dd, 1H), 3.73-3.47 (m, 3H), 3.39-3.15 (m, 2H), 3.10 (s, 1.5H), 2.99-2.85 (m, 2H), 2.68 (s, 1.5H), 2.42-2.03 (m, 9H), 1.80-1.59 (m, 2H).

[2322] Example 398. N-methyl-5-[[1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1-yl]ethyl]- 4- piperidyl]amino]-N-phenyl-quinoline-8-carboxamide

[2323] The title compound (45.5 mg, 10.4 %) was prepared in the same fashion as Example 121, except that N-methyl-N-phenyl-5-(piperidin-4-ylamino)quinoline-8-carboxa mide hydrochloride (381.3 mg, 0.88 mmol) prepared in Reference Example 230 was used instead of 3 -methyl -N- (piperidin-4-yl)quinoline-5-amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.88 (dd, 1H), 8.00 (d, 1H), 7.30-6.95 (m, 7H), 6.38 (s, 1H), 5.14-4.73 (m, 1H), 3.76-3.70 (m, 1H), 3.66-3.50 (m, 4H), 3.40-3.15 (m, 3H), 2.93-2.84 (m, 2H), 2.37-1.99 (m, 9H), 1.76-1.57 (m, 2H)

[2324] Example 399. 5-[[1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1-yl]ethyl]-4-piperid yl]amino]-N-

(2-pyridyl)quinoline-8-carboxamide

[2325] The title compound (340.4 mg, 80.0 %) was prepared in the same fashion as Example 121, except that 5-(piperidin-4-ylamino)-N-(pyridin-2-yl)quinoline-8-carboxam ide hydrochloride (369.9 mg, 0.88 mmol) prepared in Reference Example 233 was used instead of 3-methyl-N-(piperidin-4- yl)quinoline-5 -amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 9.04 (d, 1H), 8.78 (d, 1H), 8.53 (d, 1H), 8.40 (d, 1H), 8.26 (d, 1H), 7.72 (t, 1H), 7.41 (q, 1H), 7.01 (dd, 1H), 6.72 (d, 1H), 5.09-4.75 (m, 2H), 3.76-3.51 (m, 3H), 3.38-3.18 (m, 2H), 3.02-2.88 (m, 2H), 2.45-2.35 (m, 2H), 2.31-2.07 (m, 6H), 1.75-1.65 (m, 2H).

[2326] Example 400. 5-[[1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1-yl]ethyl]-4-piperid yl]amino]-N-

(3-pyridyl)quinoline-8-carboxamide

[2327] The title compound (316.8 mg, 74.4 %) was prepared in the same fashion as Example 121, except that 5-(piperidin-4-ylamino)-N-(pyridin-3-yl)quinoline-8-carboxam ide hydrochloride (369.9 mg, 0.88 mmol) prepared in Reference Example 234 was used instead of 3-methyl-N-(piperidin-4- yl)quinoline-5 -amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.92 (d, 1H), 8.84 (s, 1H), 8.84 (d, 1H), 8.46 (d, 1H), 8.31-8.29 (m, 2H), 7.41 (q, 1H), 7.29 (dd, 1H), 6.71 (dd, 1H), 5.07-4.74 (m, 2H), 3.76-3.49 (m, 3H), 3.37-3.18 (m, 2H), 3.01-2.91 (m, 2H), 2.44-2.35 (m, 2H), 2.31-2.07 (m, 6H), 1.91- 1.65 (m, 2H).

[2328] Example 401. 5-[[1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1-yl]ethyl]-4-piperid yl]amino]-N-

(4-pyridyl)quinoline-8-carboxamide

[2329] The title compound (85.1 mg, 20.0 %) was prepared in the same fashion as Example 121, except that 5-(piperidin-4-ylamino)-N-(pyridin-4-yl)quinoline-8-carboxam ide hydrochloride (369.9 mg, 0.88 mmol) prepared in Reference Example 235 was used instead of 3-methyl-N-(piperidin-4- yl)quinoline-5 -amine hydrochloride. ¹ H NMR (CDCI ₃ . 400 MHz) δ 8.97 (dd, 1H), 8.78 (d, 1H), 8.52 (d, 2H), 8.29 (d, 1H), 7.78 (d, 2H), 7.47 (q, 1H), 6.76 (d, 1H), 5.06-4.77 (m, 2H), 3.78-3.50 (m, 3H), 3.39-3.21 (m, 2H), 3.07-2.94 (m, 2H), 2.47-2.37 (m, 2H), 2.34-2.09 (m, 6H), 1.94-1.76 (m, 2H).

[2330] Example 402. 5-[[1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1-yl]ethyl]-4-piperid yl]amino]-N- pyrimidin-2-yl-quinoline-8-carboxamide

[2331] The title compound (324.4 mg, 76.0 %) was prepared in the same fashion as Example 121, except that 5-(piperidin-4-ylamino)-N-(pyrimidin-2-yl)quinoline-8-carbox amide hydrochloride (370.7 mg, 0.88 mmol) prepared in Reference Example 236 was used instead of 3-methyl-N-(piperidin-4- yl)quinoline-5 -amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 9.00 (t, 1H), 8.81 (d, 1H), 8.68 (dd, 2H), 8.29 (d, 1H), 7.41-7.39 (m, 1H), 6.98 (t, 1H), 6.70 (d, 1H), 5.09-4.99 (m, 1.4H), 5.76-4.75 (m, 0.6H), 3.72-3.51 (m, 3H), 3.48 (s, 1H), 3.34-3.17 (m, 2H), 3.00-2.87 (m, 2H), 2.43-2.34 (m, 2H), 2.30-2.08 (m, 6H), 1.75-1.65 (m, 2H).

[2332] Example 403. 5-[[1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrrolidin-1-yl]eth yl]-4- piperidyl]amino]-N-pyrimidin-2-yl-quinoline-8-carboxamide.

[2333] The title compound (16.3 mg, 29.5 %) was prepared in the same fashion as Example 131, except that 5-(piperidin-4-ylamino)-N-(pyrimidin-2-yl)quinoline-8-carbox amide hydrochloride (46.3 mg, 0.11 mmol) prepared in Reference Example 236 was used instead of 3-methyl-N-(piperidin-4- yl)quinoline-5 -amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.99 (d, 1H), 8.79 (d, 1H), 8.68 (d, 2H), 8.32-8.29 (m, 1H), 7.39 (q, 1H), 6.98 (t, 1H), 6.70-6.67 (m, 1H), 5.48-5.27 (m, 1.4H), 5.09- 4.92 (m, 1.6H), 4.20-3.72 (m, 2H), 3.62-3.56 (m, 1H), 3.48 (s, 1H), 3.43-3.17 (m, 2H), 3.01-2.87 (m, 2H), 2.49-1.83 (m, 6H), 1.75-1.55 (m, 2H).

[2334] Example 404. 5-[[1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrrolidin-1-yl]eth yl]-4- piperidyl]amino]-N-(2-pyridyl)quinoline-8-carboxamide

[2335] The title compound (45.5 mg, 82.6 %) was prepared in the same fashion as Example 131, except that 5-(piperidin-4-ylamino)-N-(pyridin-2-yl)quinoline-8-carboxam ide hydrochloride (46.3 mg, 0.11 mmol) prepared in Reference Example 233 was used instead of 3-methyl-N-(piperidin-4- yl)quinoline-5 -amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.99 (d, 1H), 8.72 (d, 1H), 8.50 (d, 1H), 8.38 (d, 1H), 8.30-8.27 (m, 1H), 7.71 (t, 1H), 7.36 (q, 1H), 7.02-6.99 (m, 1H), 6.69-6.56 (m, 1H), 5.45-5.25 (m, 1.4H), 5.12-4.90 (m, 1.6H), 4.18-3.69 (m, 2H), 3.57-3.55 (m, 1H), 3.41-3.14 (m, 2H), 3.00-2.88 (m, 2H), 2.74-2.59 (m, 1H), 2.38-2.12 (m, 7H), 1.68-1.63 (m, 2H).

[2336] Example 405. 5-[[1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrrolidin-1-yl]eth yl]-4- piperidyl]amino]-N-(3-pyridyl)quinoline-8-carboxamide

[2337] The title compound (6.8 mg, 12.3 %) was prepared in the same fashion as Example 131, except that 5-(piperidin-4-ylamino)-N-(pyridin-3-yl)quinoline-8-carboxam ide hydrochloride (46.3 mg, 0.11 mmol) prepared in Reference Example 234 was used instead of 3-methyl-N-(piperidin-4- yl)quinoline-5 -amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.92 (d, 1H), 8.85 (s, 1H), 8.44 (d, 1H), 8.31-8.29 (m, 2H), 7.41 (q, 1H), 7.30 (q, 1H), 6.72-6.68 (m, 1H), 5.49-5.29 (m, 1.4H), 5.06-4.93 (m, 1,6H), 4.23-4.13 (dd, 0.6H), 3.95-3.71 (m, 1.4H), 3.61-3.58 (m, 1H), 3.42-3.18 (m, 2H), 3.03-2.92 (m, 2H), 2.77-2.62 (m, 1H), 2.48-2.15 (m, 5H), 1,88-1.67 (m, 2H) [2338] Example 406. 5-[[1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrrolidin-1-yl]eth yl]-4- piperidyl]amino]-N-(4-pyridyl)quinoline-8-carboxamide

[2339] The title compound (1.3 mg, 2.3 %) was prepared in the same fashion as Example 131, except that 5-(piperidin-4-ylamino)-N-(pyridin-4-yl)quinoline-8-carboxam ide hydrochloride (46.3 mg, 0.11 mmol) prepared in Reference Example 235 was used instead of 3-methyl-N-(piperidin-4- yl)quinoline-5 -amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 8.98 (d, 1H), 8.80 (d, 1H), 8.53 (d, 2H), 8.28 (d, 1H), 7.78 (d, 1H), 7.48 (q, 1H), 6.77 (d, 1H), 5.50-5.30 (m, 1H), 5.29 (d, 0.4H), 4.97 (d, 0.6H), 4.86 (t, 1H), 4.23-3.66 (m, 3H), 3.44-3.21 (m, 2H), 3.06-2.95 (m, 2H), 2.81-2.66 (m,lH), 2.49-2.23 (m, 5H), 2.05-2.00 (m, 1H) 1.94-1.67 (m, 1H).

[2340] Example 407. N-isoxazol-3-yl-5-[[1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyr rolidin-1- yl]ethyl]-4-piperidyl]amino]quinoline-8-carboxamide

[2341] The title compound (20.4 mg, 37.7 %) was prepared in the same fashion as Example 131, except that N-(isoxazol-3-yl)-5-(piperidin-4-ylamino)quinoline-8-carboxa mide hydrochloride (45.3 mg, 0.11 mmol) prepared in Reference Example 237 was used instead of 3-methyl-N-(piperidin-4- yl)quinoline-5 -amine hydrochloride. ¹ H NMR (CDCI ₃ . 400 MHz) δ 8.91 (d, 1H), 8.71 (d, 1H), 8.33 (s, 1H), 8.27 (d, 1H), 7.42-7.39 (m, 1H), 7.29 (s, 1H), 6.70 (d, 1H), 5.49-5.29 (m, 1.4H), 5.03 (t, 1H),

4.94 (d, 0.6H), 4.20 (dd, 0.5H), 4.00-3.72 (m, 1.5H), 3.62-3.61 (m, 1H), 3.44-3.19 (m, 2H), 2.98 (q, 2H), 2.78-2.63 (m, 1H), 2.48-2.16 (m, 4H), 1.80-1.66 (m, 4H)

[2342] Example 408. N-[5-[[1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1-yl]ethyl]-4-pipe ridyl]amino]-

8 -quinolyl] acetamide

[2343] The title compound (33.6 mg, 9.0 %) was prepared in the same fashion as Example 121, except that N-(5-(piperidin-4-ylamino)quinolin-8-yl)acetamide hydrochloride (314.4 mg, 0.88 mmol) prepared in Reference Example 238 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) 8 δ.91 (d, 1H), 8.78 (dd, 1H), 8.60 (d, 1H), 8.20 (dd, 1H), 7.40 (q, 1H), 6.64(d, 1H) 5.49-5.27 (m, 1.4H), 4.93 (d, 0.6H), 4.23-3.73 (m, 3H), 3.48-3.42 (m, 1.4H), 3.29-3.17 (m, 1.6H), 2.99-2.87 (m, 2H), 2.77-2.61 (m, 1H), 2.47-2.33 (m, 3H), 2.30 (s,

3H), 2.21-2.13 (m, 2.5H), 1.80-1.55 (m, 2.5H)

[2344] Example 409. N-[5-[[1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrrolidin-1-yl] ethyl]-4- piperidyl]amino]-8-quinolyl]acetamide

[2345] The title compound (3.7 mg, 7.7%) was prepared in the same fashion as Example 131, except that N-(5-(piperidin-4-ylamino)quinolin-8-yl)acetamide hydrochloride (39.3 mg, 0.11 mmol) prepared in Reference Example 238 was used instead of 3-methyl-N-(piperidin-4-yl)quinoline-5- amine hydrochloride. ¹ H NMR (CDCI ₃ , 400 MHz) δ 9.49 (s, 1H), 8.78 (dd, 1H), 8.60 (d, 1H), 8.20 (dd, 1H), 7.40 (q, 1H), 6.64(d, 1H) 5.49-5.27 (m, 1.4H), 4.93 (d, 0.6H), 4.23-3.73 (m, 2.5H), 3.48- 3.25 (m, 2.5H), 3.21 (d, 1H), 2.99-2.87 (m, 2H), 2.77-2.61 (m, 1H), 2.40-2.33 (m, 2.6H), 2.30 (s, 3H), 2.21-2.13 (m, 2H), 1.83-1.55 (m, 2.4H)

[2346] Example 410. (2S,4S)-4-fluoro-1-[2-[4-[(6-methyl-4-quinolyl)oxy]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2347] The title compound (190.9 mg, 38.8 %) was prepared in the same fashion as Example 330, except that 6-methyl-4-(piperidin-4-yloxy)quinoline hydrochloride (346.3 mg, 1.24 mmol) prepared in Reference Example 239 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.65 (d, 1H), 7.97 (s, 1H), 7.92 (d, 1H), 7.53 (d, 1H), 6.69 (d, 1H), 5.50-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.75-4.60 (m, 1H), 4.30-4.10 (m, 1H), 4.05-3.70 (m, 2H), 3.60-3.20 (m, 2H), 2.90-2.80 (m, 2H), 2.80-2.65 (m, 1H), 2.65-2.55 (m, 2H), 2.55 (s, 3H), 2.40-2.05 (m, 2H), 2.05-1.95 (m, 2H)

[2348] Example 411. (2S,4S)-4-fluoro-1-[2-[4-[[6-(trifluoromethyl)-4-quinolyl]ox y]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2349] The title compound (245.4 mg, 43.9 %) was prepared in the same fashion as Example 330, except that 4-(piperidin-4-yloxy)-6-(trifluoromethyl)quinoline hydrochloride (413.3 mg, 1.24 mmol) prepared in Reference Example 240 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.83 (d, 1H), 8.51 (s, 1H), 8.13 (d, 1H), 7.87 (d, 1H), 6.81 (d, 1H), 5.55-5.35 (m, 1H), 5.00-4.90 (m, 1H), 4.75-4.65 (m, 1H), 4.30-4.10 (m, 1H), 4.05-3.70 (m, 2H), 3.55-3.20 (m, 2H), 3.00-2.80 (m, 2H), 2.80-2.60 (m, 1H), 2.60-2.50 (m, 2H), 2.30-2.10 (m, 2H), 2.10-1.95 (m, 2H)

[2350] Example 412. (2S,4S)-4-fluoro-1-[2-[4-[[2-(trifluoromethyl)-4-quinolyl]ox y]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2351] The title compound (312.7 mg, 55.9 %) was prepared in the same fashion as Example 330, except that 4-(piperidin-4-yloxy)-2-(trifluoromethyl)quinoline hydrochloride (291.3 mg, 0.88 mmol) prepared in Reference Example 241 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.27 (d, 1H), 8.14 (d, 1H), 7.80 (t, 1H), 7.62 (t, 1H),

7.02 (s, 1H), 5.50-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.85-4.70 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.70 (m, 2H), 3.55-3.15 (m, 2H), 2.95-2.80 (m, 2H), 2.80-2.65 (m, 1H), 2.65-2.55 (m, 2H), 2.30-2.10 (m, 2H), 2.10-1.95 (m, 2H)

[2352] Example 413. (2S,4S)-4-fluoro-1-[2-[4-[[6-fluoro-2-(trifluoromethyl)-4-qu inolyl]oxy]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2353] The title compound (243.7 mg, 41.9 %) was prepared in the same fashion as Example 330, except that 6-fluoro-4-(piperidin-4-yloxy)-2-(trifluoromethyl)quinoline hydrochloride (255.6 mg, 0.73 mmol) prepared in Reference Example 242 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5- amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.15 (d, 1H), 7.84 (d, 1H), 7.56 (s, 1H), 7.03 (s, 1H), 5.50-5.20 (m, 1H), 5.00-4.90 (m, 1H), 4.85-4.70 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.70 (m, 2H), 3.55-3.20 (m, 2H), 2.95-2.80 (m, 2H), 2.80-2.65 (m, 1H), 2.65-2.55 (m, 2H), 2.30-2.10 (m, 2H), 2.10- 1.95 (m, 2H)

[2354] Example 414. (2S,4S)-4-fluoro-1-[2-[4-[[6-methoxy-2-(trifluoromethyl)-4-q uinolyl]oxy]- 1-piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2355] The title compound (266.4 mg, 44.6 %) was prepared in the same fashion as Example 330, except that 6-methoxy-4-(piperidin-4-yloxy)-2-(trifluoromethyl)quinoline hydrochloride (303.8 mg, 0.84 mmol) prepared in Reference Example 243 was used instead of 8-fluoro-N-(piperidin-4- yl)quinolin-5 -amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.04 (d, 1H), 7.48-7.42 (m, 2H), 7.00 (s, 1H), 5.55-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.85-4.70 (m, 1H), 4.35-4.20 (m, 1H), 3.98 (s,

3H), 3.98-3.70 (m, 2H), 3.50-3.20 (m, 2H), 2.95-2.85 (m, 2H), 2.85-2.60 (m, 1H), 2.70-2.55 (m, 2H), 2.30-2.10 (m, 2H), 2.10-2.00 (m, 2H)

[2356] Example 415. (2S,4S)-4-fluoro-1-[2-[4-[[8-methoxy-2-(trifluoromethyl)-4-q uinolyl]oxy]- l-piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2357] The title compound (429.0 mg, 71.9 %) was prepared in the same fashion as Example 330, except that 8-methoxy-4-(piperidin-4-yloxy)-2-(trifluoromethyl)quinoline hydrochloride (414.9 mg, 1.14 mmol) prepared in Reference Example 244 was used instead of 8-fluoro-N-(piperidin-4- yl)quinolin-5 -amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 7.81 (d, 1H), 7.54 (t, 1H), 7.14 (d, 1H), 7.05 (s, 1H), 5.55-5.35 (m, 1H), 5.00-4.90 (m, 1H), 4.85-4.70 (m, 1H), 4.30-4.15 (m, 1H), 4.09 (s, 3H), 4.05-3.70 (m, 2H), 3.55-3.20 (m, 2H), 2.90-2.80 (m, 2H), 2.80-2.70 (m, 1H), 2.70-2.55 (m, 2H), 2.35-2.10 (m, 2H), 2.10-2.00 (m, 2H)

[2358] Example 416. (2S,4S)-4-fluoro-1-[2-[4-[(3-fluoro-5-quinolyl)oxy]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2359] The title compound (179.0 mg, 36.0 %) was prepared in the same fashion as Example 330, except that 3-fluoro-5-(piperidin-4-yloxy)quinoline hydrochloride (351.2 mg, 1.24 mmol) prepared in

Reference Example 245 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.79 (s, 1H), 8.17 (d, 1H), 7.68 (s, 1H), 7.56 (t, 1H),

6.91 (d, 1H), 5.55-5.30 (m, 1H), 5.05-4.85 (m, 1H), 4.70-4.50 (m, 1H), 4.30-4.10 (m, 1H), 4.00-3.70 (m, 2H), 3.55-3.20 (m, 2H), 2.90-2.75 (m, 2H), 2.75-2.60 (m, 1H), 2.60-2.50 (m, 2H), 2.35-2.10 (m, 2H), 2.10-1.90 (m, 2H) [2360] Example 417. 5-[[1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrrolidin-1-yl]eth yl]-4- piperidyl]oxy]quinoline-2-carbonitrile

[2361] The title compound (70.2 mg, 13.9 %) was prepared in the same fashion as Example 330, except that 5-(piperidin-4-yloxy)quinoline-2-carbonitrile hydrochloride (359.9 mg, 1.24 mmol) prepared in Reference Example 246 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.73 (d, 1H), 7.73-7.64 (m, 3H), 6.99 (d, 1H), 5.55-5.35 (m, 1H), 5.00-4.90 (m, 1H), 4.70-4.55 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.70 (m, 2H), 3.55-3.20 (m, 2H), 2.90-2.80 (m, 2H), 2.80-2.65 (m, 1H), 2.65-2.50 (m, 2H), 2.35-2.05 (m, 2H), 2.05-1.95 (m, 2H)

[2362] Example 418. (2S,4S)-1-[2-[4-[(8-chloro-5-quinolyl)oxy]-1-piperidyl]acety l]-4-fluoro- pyrrolidine-2-carbonitrile

[2363] The title compound (304.0 mg, 58.7 %) was prepared in the same fashion as Example 330, except that 8-chloro-5-(piperidin-4-yloxy)quinoline hydrochloride (371.7 mg, 1.24 mmol) prepared in

Reference Example 247 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.99 (d, 1H), 8.53 (d, 1H), 7.53-7.50 (m, 2H), 7.04 (d, 1H), 5.75-5.20 (m, 1H), 5.00-4.90 (m, 1H), 4.70-4.50 (m, 1H), 4.30-4.10 (m, 1H), 4.10-3.65 (m, 2H), 3.65-3.20 (m, 2H), 3.10-2.80 (m, 2H), 2.80-2.55 (m, 1H), 2.55-2.40 (m, 2H), 2.40-2.15 (m, 2H), 2.15- 1.90 (m, 2H)

[2364] Example 419. (2S,4S)-4-fluoro-1-[2-[4-(l-isoquinolyloxy)-1-piperidyl]acet yl]pyrrolidine-

2-carbonitrile

[2365] The title compound (156.9 mg, 86.5 %) was prepared in the same fashion as Example 330, except that l-(piperidin-4-yloxy)isoquinoline hydrochloride (125.6 mg, 0.47 mmol) prepared in

Reference Example 248 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.25 (d, 1H), 7.96 (d, 1H), 7.73 (d, 1H), 7.66 (t, 1H), 7.54 (t, 1H), 7.19 (d, 1H), 5.75-5.25 (m, 2H), 5.00-4.90 (m, 1H), 4.30-4.15 (m, 1H), 4.10-3.70 (m, 2H), 3.60-3.20 (m, 2H), 2.90-2.80 (m, 2H), 2.80-2.65 (m, 1H), 2.65-2.50 (m, 2H), 2.35-2.10 (m, 2H), 2.10-1.90 (m, 2H)

[2366] Example 420. (2S,4S)-4-fluoro-1-[2-[4-(4-isoquinolyloxy)-1-piperidyl]acet yl]pyrrolidine-

2-carbonitrile

[2367] The title compound (39.9 mg, 22.0 %) was prepared in the same fashion as Example 330, except that 4-(piperidin-4-yloxy)isoquinoline hydrochloride (328.9 mg, 1.24 mmol) prepared in

Reference Example 249 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.90 (s, 1H), 8.22 (d, 1H), 8.10 (s, 1H), 7.94 (d, 1H), 7.71 (t, 1H), 7.63 (t, 1H), 5.60-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.80-4.60 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.70 (m, 2H), 3.60-3.15 (m, 2H), 2.95-2.80 (m, 2H), 2.80-2.60 (m, 1H), 2.60-2.50 (m, 2H),

2.30-2.10 (m, 2H), 2.10-1.90 (m, 2H)

[2368] Example 421. (2S,4S)-4-fluoro-1-[2-[4-(5-isoquinolyloxy)-1-piperidyl]acet yl]pyrrolidine-

2-carbonitrile

[2369] The title compound (107.7 mg, 59.4 %) was prepared in the same fashion as Example 330, except that 5-(piperidin-4-yloxy)isoquinoline hydrochloride (156.5 mg, 0.59 mmol) prepared in

Reference Example 250 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 9.20 (s, 1H), 8.53 (d, 1H), 8.03 (d, 1H), 7.55-7.47 (m, 2H), 7.02 (d, 1H), 5.55-5.20 (m, 1H), 5.00-4.85 (m, 1H), 4.70-4.50 (m, 1H), 4.30-4.15 (m, 1H), 4.05- 3.70 (m, 2H), 3.60-3.20 (m, 2H), 2.90-2.80 (m, 2H), 2.80-2.60 (m, 1H), 2.60-2.50 (m, 2H), 2.30-2.10 (m, 2H), 2.10-1.90 (m, 2H)

[2370] Example 422. (2S,4S)-4-fluoro-1-[2-[4-(6-isoquinolyloxy)-1-piperidyl]acet yl]pyrrolidine-

2-carbonitrile

[2371] The title compound (73.3 mg, 40.4 %) was prepared in the same fashion as Example 330, except that 6-(piperidin-4-yloxy)isoquinoline hydrochloride (328.9 mg, 1.24 mmol) prepared in

Reference Example 251 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 9.10 (s, 1H), 8.43 (d, 1H), 7.87 (d, 1H), 7.52 (d, 1H), 7.23 (d, 1H), 7.06 (s, 1H), 5.55-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.65-4.50 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.70 (m, 2H), 3.60-3.15 (m, 2H), 2.90-2.80 (m, 2H), 2.80-2.60 (m, 1H), 2.60-2.45 (m, 2H),

2.30-2.10 (m, 2H), 2.00-1.80 (m, 2H)

[2372] Example 423. (2S,4S)-4-fluoro-1-[2-[4-(7-isoquinolyloxy)-1-piperidyl]acet yl]pyrrolidine-

2-carbonitrile

[2373] The title compound (140.7 mg, 77.6 %) was prepared in the same fashion as Example 330, except that 7-(piperidin-4-yloxy)isoquinoline hydrochloride (328.9 mg, 1.24 mmol) prepared in

Reference Example 252 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 9.15 (s, 1H), 8.41 (d, 1H), 7.75 (d, 1H), 7.58 (d, 1H), 7.35 (d, 1H), 7.23 (s, 1H), 5.55-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.60-4.45 (m, 1H), 4.25-4.10 (m, 1H), 4.05-3.70 (m, 2H), 3.65-3.20 (m, 2H), 2.90-2.80 (m, 2H), 2.80-2.60 (m, 1H), 2.60-2.45 (m, 2H),

2.30-2.05 (m, 2H), 2.00-1.85 (m, 2H)

[2374] Example 424. (2S,4S)-4-fluoro-1-[2-[4-[(6-methoxy-2-methyl-4-quinolyl)oxy ]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2375] The title compound (141.2 mg, 69.8 %) was prepared in the same fashion as Example 330, except that 6-methoxy-2-methyl-4-(piperidin-4-yloxy)quinoline hydrochloride (383.6 mg, 1.24 mmol) prepared in Reference Example 253 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 7.85 (d, 1H), 7.42 (s, 1H), 7.32 (d, 1H), 6.59 (s, 1H), 5.55-5.20 (m, 1H), 5.00-4.90 (m, 1H), 4.70-4.60 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.70 (m, 2H), 3.94 (s, 3H), 3.55-3.20 (m, 2H), 2.90-2.80 (m, 2H), 2.80-2.65 (m, 1H), 2.65 (s, 3H), 2.65-2.50 (m, 2H), 2.30-2.10 (m, 2H), 2.10-1.95 (m, 2H)

[2376] Example 425. (2S,4S)-4-fluoro-1-[2-[4-(5-quinolyloxy)-1-piperidyl]acetyl] pyrrolidine-2- carbonitrile

[2377] The title compound (161.4 mg, 37.0 %) was prepared in the same fashion as Example 330, except that 5-(piperidin-4-yloxy)quinoline hydrochloride (383.6 mg, 1.24 mmol) prepared in

Reference Example 33 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.91 (d, 1H), 8.60 (d, 1H), 7.69 (d, 1H), 7.60 (t, 1H), 7.40 (t, 1H), 6.89 (d, 1H), 5.55-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.80-4.70 (m, 1H), 4.30-4.15 (m,

1H), 4.05-3.70 (m, 2H), 3.55-3.20 (m, 2H), 2.95-2.80 (m, 2H), 2.80-2.65 (m, 1H), 2.65-2.50 (m, 2H), 2.30-2.10 (m, 2H), 2.10-1.95 (m, 2H)

[2378] Example 426. (2S)-1-[2-[4-[(6-methoxy-4-quinolyl)oxy]-1-piperidyl]acetyl] pyrrolidine-2- carbonitrile

[2379] The title compound (127.1 mg, 25.9 %) was prepared in the same fashion as Example 110, except that 6-methoxy-4-(piperidin-4-yloxy)quinoline hydrochloride (366.1 mg, 1.24 mmol) prepared in Reference Example 42 was used instead of N-(piperidin-4-yl)quinoline-5-amine hydrochloride. ¹ H- NMR (CDCI ₃ , 400MHz) δ 8.59 (d, 1H), 7.93 (d, 1H), 7.47 (s, 1H), 7.34 (d, 1H), 6.70 (d, 1H), 5.20- 4.70 (m, 1H), 4.70-4.60 (m, 1H), 3.95 (s, 3H), 3.80-3.60 (m, 1H), 3.60-3.50 (m, 1H), 3.45-3.20 (m, 2H), 2.95-2.75 (m, 2H), 2.70-2.50 (m, 2H), 2.40-2.10 (m, 6H), 2.10-1.95 (m, 2H)

[2380] Example 427. (2S)-1-[2-[4-[(6-fluoro-4-quinolyl)oxy]-1-piperidyl]acetyl]p yrrolidine-2- carbonitrile

[2381] The title compound (51.1 mg, 10.8 %) was prepared in the same fashion as Example 110, except that 6-fluoro-4-(piperidin-4-yloxy)quinoline hydrochloride (351.2 mg, 1.24 mmol) prepared in

Reference Example 40 was used instead of N-(piperidin-4-yl)quinoline-5-amine hydrochloride. ¹ H- NMR (CDCI ₃ , 400MHz) δ 8.69 (d, 1H), 8.02 (d, 1H), 7.81 (d, 1H), 7.46 (s, 1H), 6.74 (d, 1H), 5.20- 4.75 (m, 1H), 4.75-4.60 (m, 1H), 3.80-3.60 (m, 1H), 3.60-3.50 (m, 1H), 3.50-3.20 (m, 2H), 2.95-2.75 (m, 2H), 2.70-2.50 (m, 2H), 2.40-2.10 (m, 6H), 2.10-1.95 (m, 2H)

[2382] Example 428. (2S)-1-[2-[4-[(7-chloro-4-quinolyl)oxy]-1-piperidyl]acetyl]p yrrolidine-2- carbonitrile

[2383] The title compound (272.5 mg, 55.0 %) was prepared in the same fashion as Example 110, except that 7-chloro-4-(piperidin-4-yloxy)quinoline hydrochloride (371.6 mg, 1.24 mmol) prepared in

Reference Example 254 was used instead of N-(piperidin-4-yl)quinoline-5-amine hydrochloride. ¹ H- NMR (CDC1 ₃ , 400MHz) δ 8.70 (d, 1H), 8.14 (d, 1H), 8.00 (s, 1H), 7.43 (d, 1H), 6.70 (d, 1H), 5.20- 4.75 (m, 1H), 4.75-4.60 (m, 1H), 3.80-3.60 (m, 1H), 3.60-3.50 (m, 1H), 3.45-3.15 (m, 2H), 2.90-2.75 (m, 2H), 2.70-2.50 (m, 2H), 2.40-2.10 (m, 6H), 2.10-1.95 (m, 2H)

[2384] Example 429. (2S)-1-[2-[4-[(3-fluoro-5-quinolyl)oxy]-1-piperidyl]acetyl]p yrrolidine-2- carbonitrile

[2385] The title compound (419.4 mg, 88.3 %) was prepared in the same fashion as Example 110, except that 3-fluoro-5-(piperidin-4-yloxy)quinoline hydrochloride (351.2 mg, 1.24 mmol) prepared in

Reference Example 245 was used instead of N-(piperidin-4-yl)quinoline-5-amine hydrochloride. ¹ H- NMR (CDC1 ₃ , 400MHz) δ 8.79 (s, 1H), 8.19 (s, 1H), 7.68 (d, 1H), 7.55 (t, 1H), 6.91 (d, 1H), 5.25- 4.70 (m, 1H), 4.65-4.55 (m, 1H), 3.80-3.60 (m, 1H), 3.60-3.50 (m, 1H), 3.50-3.10 (m, 2H), 2.90-2.75 (m, 2H), 2.65-2.50 (m, 2H), 2.45-2.10 (m, 6H), 2.10-1.95 (m, 2H)

[2386] Example 430. 5-[[1-[2-oxo-2-[(2S)-2-cyanopyrrolidin-1-yl]ethyl]-4- piperidyl]oxy]quinoline-2-carbonitrile

[2387] The title compound (198.9 mg, 41.1 %) was prepared in the same fashion as Example 110, except that 5-(piperidin-4-yloxy)quinoline-2-carbonitrile hydrochloride (359.9 mg, 1.24 mmol) prepared in Reference Example 246 was used instead of N-(piperidin-4-yl)quinoline-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.74 (d, 1H), 7.72-7.66 (m, 3H), 7.01 (d, 1H), 5.20-4.75 (m, 1H), 4.70-4.60 (m, 1H), 3.80-3.60 (m, 1H), 3.60-3.50 (m, 1H), 3.45-3.20 (m, 2H), 2.90-2.80 (m, 2H), 2.70-2.50 (m, 2H), 2.40-2.10 (m, 6H), 2.10-1.95 (m, 2H)

[2388] Example 431. (2S)-1-[2-[4-[(8-chloro-5-quinolyl)oxy]-1-piperidyl]acetyl]p yrrolidine-2- carbonitrile

[2389] The title compound (383.5 mg, 77.4 %) was prepared in the same fashion as Example 110, except that 8-chloro-5-(piperidin-4-yloxy)quinoline hydrochloride (371.6 mg, 1.24 mmol) prepared in

Reference Example 247 was used instead of N-(piperidin-4-yl)quinoline-5-amine hydrochloride. ¹ H- NMR (CDC1 ₃ , 400MHz) δ 9.00 (d, 1H), 8.54 (d, 1H), 7.56-7.51 (m, 2H), 7.05 (d, 1H), 5.45-4.70 (m, 1H), 4.65-4.55 (m, 1H), 3.85-3.65 (m, 1H), 3.65-3.55 (m, 1H), 3.55-3.20 (m, 2H), 3.05-2.85 (m, 2H), 2.60-2.40 (m, 2H), 2.40-2.00 (m, 8H)

[2390] Example 432. (2S,4S)-4-fluoro-1-[2-[4-[(7-methyl-4-quinolyl)oxy]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2391] The title compound (129.3 mg, 22.3 %) was prepared in the same fashion as Example 330, except that 7-methyl-4-(piperidin-4-yloxy)quinoline hydrochloride (407.0 mg, 1.46 mmol) prepared in Reference Example 255 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.68 (d, 1H), 8.11 (d, 1H), 7.80 (s, 1H), 7.34 (d, 1H), 6.67 (d, 1H), 5.50-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.75-4.65 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.70 (m, 2H), 3.55-3.20 (m, 2H), 2.95-2.75 (m, 2H), 2.75-2.65 (m, 1H), 2.65-2.56 (m, 2H), 2.56 (s, 3H), 2.30-2.10 (m, 2H), 2.10-1.95 (m, 2H)

[2392] Example 433. (2S,4S)-4-fluoro-1-[2-[4-[(7-fluoro-4-quinolyl)oxy]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2393] The title compound (239.1 mg, 40.9 %) was prepared in the same fashion as Example 330, except that 7-fluoro-4-(piperidin-4-yloxy)quinoline hydrochloride (421.3 mg, 1.49 mmol) prepared in

Reference Example 256 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.71 (d, 1H), 8.22 (d, 1H), 7.64 (d, 1H), 7.27 (s, 1H), 6.69 (d, 1H), 5.50-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.75-4.65 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.70 (m, 2H), 3.50-3.20 (m, 2H), 2.95-2.80 (m, 2H), 2.80-2.65 (m, 1H), 2.65-2.50 (m, 2H), 2.30-2.10 (m, 2H), 2.10-1.95 (m, 2H)

[2394] Example 434. 4-[[1-[2-oxo-2-[(2S,4S)-2-cyano-4-fluoro-pyrrolidin-1-yl]eth yl]-4- piperidyl]oxy] quinoline- 8 -carbonitrile

[2395] The title compound (277.0 mg, 46.6 %) was prepared in the same fashion as Example 330, except that 4-(piperidin-4-yloxy)quinoline-8-carbonitrile hydrochloride (414.4 mg, 1.43 mmol) prepared in Reference Example 257 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.87 (d, 1H), 8.46 (d, 1H), 8.09 (d, 1H), 7.54 (t, 1H), 6.84 (d, 1H), 5.55-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.80-4.65 (m, 1H), 4.30-4.15 (m, 1H), 4.00-3.70 (m, 2H), 3.50-3.20 (m, 2H), 2.90-2.80 (m, 2H), 2.80-2.65 (m, 1H), 2.65-2.50 (m, 2H), 2.30-2.10 (m, 2H), 2.10-1.95 (m, 2H)

[2396] Example 435. (2S,4S)-4-fluoro-1-[2-[4-[(8-fluoro-4-quinolyl)oxy]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2397] The title compound (301.2 mg, 50.5 %) was prepared in the same fashion as Example 330, except that 8-fluoro-4-(piperidin-4-yloxy)quinoline hydrochloride (421.3 mg, 1.49 mmol) prepared in

Reference Example 258 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDCI ₃ , 400MHz) δ 8.77 (d, 1H), 8.00 (d, 1H), 7.44-7.37 (m, 2H), 6.78 (d, 1H), 5.55-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.80-4.65 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.70 (m, 2H), 3.55-3.20 (m, 2H), 2.95-2.75 (m, 2H), 2.75-2.65 (m, 1H), 2.65-2.50 (m, 2H), 2.30-2.10 (m, 2H), 2.10- 1.95 (m, 2H)

[2398] Example 436. (2S,4S)-4-fluoro-1-[2-[4-[(2-methyl-4-quinolyl)oxy]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2399] The title compound (79.9 mg, 13.5 %) was prepared in the same fashion as Example 330, except that 2-methyl -4-(piperidin-4-yloxy)quinoline hydrochloride (415.4 mg, 1.49 mmol) prepared in Reference Example 259 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.16 (d, 1H), 7.94 (d, 1H), 7.66 (t, 1H), 7.44 (t, 1H),

6.61 (s, 1H), 5.50-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.75-4.65 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.70 (m, 2H), 3.55-3.20 (m, 2H), 2.95-2.80 (m, 2H), 2.80-2.69 (m, 1H), 2.69 (s, 3H), 2.69-2.55 (m, 2H), 2.30-2.10 (m, 2H), 2.10-1.95 (m, 2H)

[2400] Example 437. (2S,4S)-4-fluoro-1-[2-[4-[(6-fluoro-2-methyl-4-quinolyl)oxy] -1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2401] The title compound (403.6 mg, 65.4 %) was prepared in the same fashion as Example 330, except that 6-fluoro-2-methyl-4-(piperidin-4-yloxy)quinoline hydrochloride (442.2 mg, 1.49 mmol) prepared in Reference Example 260 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 7.90 (d, 1H), 7.72 (d, 1H), 7.39 (s, 1H), 6.60 (s, 1H), 5.50-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.70-4.60 (m, 1H), 4.30-4.15 (m, 1H), 4.05-3.70 (m, 2H), 3.50- 3.20 (m, 2H), 2.90-2.75 (m, 2H), 2.75-2.65 (m, 1H), 2.65 (s, 3H), 2.65-2.55 (m, 2H), 2.30-2.10 (m, 2H), 2.10-1.95 (m, 2H)

[2402] Example 438. (2S,4S)-4-fluoro-1-[2-[4-[(8-methoxy-4-quinolyl)oxy]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2403] The title compound (65.9 mg, 10.7 %) was prepared in the same fashion as Example 330, except that 8-methoxy-4-(piperidin-4-yloxy)quinoline hydrochloride (97.6 mg, 0.33 mmol) prepared in Reference Example 261 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.74 (d, 1H), 7.79 (d, 1H), 7.43 (t, 1H), 7.06 (d, 1H), 6.76 (d, 1H), 5.50-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.75-4.65 (m, 1H), 4.30-4.15 (m, 1H), 4.08 (s, 3H), 4.08-3.70 (m, 2H), 3.55-3.20 (m, 2H), 2.90-2.75 (m, 2H), 2.75-2.65 (m, 1H), 2.65-2.55 (m, 2H), 2.30-2.10 (m, 2H), 2.10-1.95 (m, 2H)

[2404] Example 439. (2S,4S)-4-fluoro-1-[2-[4-[(8-methyl-4-quinolyl)oxy]-1- piperidyl]acetyl]pyrrolidine-2-carbonitrile

[2405] The title compound (450.4 mg, 76.3 %) was prepared in the same fashion as Example 330, except that 8-methyl -4-(piperidin-4-yloxy)quinoline hydrochloride (415.4 mg, 1.49 mmol) prepared in Reference Example 262 was used instead of 8-fluoro-N-(piperidin-4-yl)quinolin-5-amine hydrochloride. ¹ H-NMR (CDC1 ₃ , 400MHz) δ 8.75 (d, 1H), 8.08 (d, 1H), 7.54 (d, 1H), 7.38 (t, 1H), 6.72 (d, 1H), 5.50-5.25 (m, 1H), 5.00-4.90 (m, 1H), 4.75-4.65 (m, 1H), 4.25-4.10 (m, 1H), 4.05-3.70 (m, 2H), 3.50-3.20 (m, 2H), 2.90-2.78 (m, 2H), 2.78 (s, 3H), 2.78-2.60 (m, 1H), 2.60-2.50 (m, 2H), 2.30-2.10 (m, 2H), 2.10-1.95 (m, 2H)

[2406] Compound structures are provided in Table 1. Chemical names are as determined by ChemDraw.

[2407] Table 1.

[2408] Experimental Example 1 : Evaluation of the inhibitory effect on the activity of dipeptidyl peptidases including FAP

[2409] The compounds according to the present technology were evaluated in terms of the inhibitory effect on the activity of dipeptidyl peptidases including FAP by using a substrate producing fluorescent substances upon enzymatic decomposition. The test was conducted at 25 °C to get a final reaction volume of 100 μL in a 96 black well plate. Each of the dipeptidyl peptidases was reacted with test compounds (compounds according to the present technology) or Talabostat for 10 minutes and then decomposed by enzymes for 10 minutes to measure the level of the fluorescent substances released therefrom. Briefly, the test compounds were dissolved in dimethyl sulfoxide (DMSO) at a concentration of 20 mM and then subjected to serial dilution, thereby creating an 8 point curve. As for the control group, a 6 point curve created by serial dilution of Talabostat in the same buffer solution was used. Fluorescence intensity was measured using a microplate reader (Flexstation3, Molecular Devices) under the wavelength condition exciting at 380 nm and reading the emission at 460 nm. The inhibitory effect of the test compounds was measured as a decrease (%) in the signal rate as compared to the control group without any inhibitor. Data was fixed to a logistic model with four variables and IC50 value was calculated using GraphPad Prism program.

[2410] recombinant human FAP (R&D systems) was diluted with 50 mM tris (pH 7.5) buffer comprising 1 M of sodium chloride and 1 mg/ml of bovine serum a1bumin (BSA) to be 10 ng per final reaction. The test compounds were subjected to serial dilution in a buffer under the condition containing up to 0.1 % of DMSO. Z-Gly-Pro-AMC (Bachem) was used as a substrate. By dissolving in DMSO and then diluting in the same buffer, the substrate was added to be 50 pm per final reaction.

[2411] As for recombinant human DPP4 (R&D Systems), 25 mM tris (pH 8.0) buffer as a buffer solution and H-Gly-Pro-AMC (Bachem) as a substrate were used. As for recombinant human DPP7 (R&D Systems), 25 mM MES (pH 6.0) buffer as a buffer solution and Lys-Pro-AMC (Bachem) as a substrate were used. As for recombinant human DPP8 (PBS Bioscience), as a buffer solution, a 10 mM tris (pH 7.4) buffer comprising 10 mM of magnesium chloride and 0.05 % of Tween 20, and Z- Ala-Pro-AMC (BPS Bioscience) as a substrate were used. As for recombinant human DPP9 (R&D Systems), a 25 mM tris (pH 8.0) buffer as a buffer solution and H-Gly-Pro-AMC (Bachem) as a substrate were used. As for recombinant human PREP (R&D System), as a buffer solution, a 25 mM tris (pH 7.5) buffer comprising 250 mM sodium chloride and 2.5 mM DTT (Dithiolthreitol), and Z- Gly-Pro-AMC (Bachem) as a substrate were used. The evaluation was made by using 10 ng of DPP4, PDD7, and PREP, 20 ng of DPP8, and 50 ng of DPP9 based on the final reaction. In the evaluation of DPP4, the test compounds were subjected to serial dilution in a buffer under the condition containing up to 0.1 % of DMSO. In the evaluation of DPP7, DPP8, DPP9, and PREP, the test compounds were subjected to serial dilution in each buffer under the condition containing up to 0.5 % of DMSO. [2412] For the FAP FRET assay, recombinant human FAP (R&D systems) was diluted with 50 mM HEPES (pH 7.2) buffer comprising 150 mM of sodium chloride, 0.1 mg/ml of bovine serum a1bumin (BSA) and 1 mM EDTAto be 10 ng per final reaction. The test compounds were subjected to serial dilution in a buffer under the condition containing up to 0.1 % of DMSO. FRET peptides containing human FGF21 sequence (VGPSQGK) conjugated with HyLite Fluor 488 and dark quencher QXL 520 (Anaspec) was used as a substrate. By dissolving in DMSO and then diluting in the same buffer, the substrate was added to be 6 mM per final reaction. All reactions of FRET assay was conducted at 37 °C.

[2413] The results obtained by evaluating the inhibitory effect on the activity of dipeptidyl peptidase including FAP as above are shown in Tables 2-8 below.

[2414] Table 2.

*IC ₅₀ value is between 1 and 30 nM.; ** is inhibition% at ImM [2415] Table 3.

*IC ₅₀ value is between 1 and 30 nM.; ** is inhibition% at ImM

[2416] Table 4.

*IC ₅₀ value is between 1 and 30 nM.; ** is inhibition% at ImM

[2417] Table 5.

*IC ₅₀ value is between 1 and 30 nM.; ** is inhibition% at ImM [2418] Table 6.

*IC ₅₀ value is between 1 and 30 nM.; ** is inhibition% at ImM

[2419] Table 7.

[2420] Table 8.