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Title:
QUANTIFICATION METHOD FOR 25-HYDROXYVITAMIN D, HYDROXYLASE, COMPOSITION FOR QUANTIFICATION, KIT FOR QUANTIFICATION, ELECTRODE, SENSOR CHIP, AND SENSOR
Document Type and Number:
WIPO Patent Application WO/2022/264946
Kind Code:
A1
Abstract:
Provided are: a new quantification method for measuring 25-hydroxyvitamin D; a hydroxylase; a composition for quantification; a kit for quantification; an electrode; a sensor chip; and a sensor. According to the present invention, it is possible to provide a quantification method for 25-hydroxyvitamin D, the method comprising adding a mediator and a hydroxylase to a sample. The method may comprise quantifying an oxidized-form mediator generated by the action of the hydroxylase, or quantifying a reduced-form mediator or oxygen being consumed.

Inventors:
HAYASHI KANAKO (JP)
ICHIYANAGI ATSUSHI (JP)
Application Number:
PCT/JP2022/023533
Publication Date:
December 22, 2022
Filing Date:
June 10, 2022
Export Citation:
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Assignee:
KIKKOMAN CORP (JP)
International Classes:
C12M1/34; C12N9/14; C12Q1/00; C12Q1/34; G01N27/327; G01N27/416
Domestic Patent References:
WO2007138894A12007-12-06
WO2010014978A12010-02-04
Other References:
ANONYMOUS: "Report KAKENHI-PROJECT-25292062", 10 May 2017 (2017-05-10), XP093015946, Retrieved from the Internet [retrieved on 20230120]
ISHII AKIO, SEIYA TSUJIMURA, KENJI KANO: "Dependence of Steady-State Catalytic Current on the Thickness of an Enzyme-Mediator-Immobilized Layer Fabricated by Layer-by-Layer Method", BUNSEKI KAGAKU, vol. 56, no. 6, 30 June 2007 (2007-06-30), pages 419 - 424, XP093015955, DOI: 10.2116/bunsekikagaku.56.419
MIE YASUHIRO; YASUTAKE YOSHIAKI; TAKAYAMA HARUKA; TAMURA TOMOHIRO: "Electrochemically boosted cytochrome P450 reaction that efficiently produces 25-hydroxyvitamin D3", JOURNAL OF CATALYSIS, ACADEMIC PRESS, DULUTH, MN., US, vol. 384, 28 February 2020 (2020-02-28), US , pages 30 - 36, XP086128173, ISSN: 0021-9517, DOI: 10.1016/j.jcat.2020.02.012
Attorney, Agent or Firm:
TAKAHASHI, HAYASHI AND PARTNER PATENT ATTORNEYS, INC. (JP)
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