Title:
QUANTIFYING MRNA OF PROTEINS OF INTEREST USING A REAL-TIME RT-PCR REACTION
Document Type and Number:
WIPO Patent Application WO2006024737
Kind Code:
A3
Abstract:
The invention concerns the field of quantification of RNA's in a biological sample, in particular quantification of messenger RNA's coding for cytokines. The invention enables the level of messenger RNA's of human cytokines present in a biological sample to be absolutely quantified, through a real-time RT-PCR technique based on the use of highly specific primers, the detection of amplifications by incorporating a fluorescent intercalator, and the absolute quantification by using internal (constitutive genes) and external reference RNA standard range) norms. The invention concerns the simultaneous quantification of different RNA's, in the same experimental conditions.
Inventors:
BEHR CHARLOTTE (FR)
BOEUF PHILIPPE (AU)
BOEUF PHILIPPE (AU)
Application Number:
PCT/FR2005/001904
Publication Date:
August 31, 2006
Filing Date:
July 22, 2005
Export Citation:
Assignee:
CENTRE NAT RECH SCIENT (FR)
PASTEUR INSTITUT (FR)
BEHR CHARLOTTE (FR)
BOEUF PHILIPPE (AU)
PASTEUR INSTITUT (FR)
BEHR CHARLOTTE (FR)
BOEUF PHILIPPE (AU)
International Classes:
C12Q1/68
Domestic Patent References:
| WO1999054510A2 | 1999-10-28 | |||
| WO2000020629A2 | 2000-04-13 | |||
| WO2003060119A2 | 2003-07-24 | |||
| WO1996039536A1 | 1996-12-12 |
Foreign References:
| FR2842211A1 | 2004-01-16 |
Other References:
STORDEUR PATRICK ET AL: "Cytokine mRNA quantification by real-time PCR", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 259, no. 1-2, 1 January 2002 (2002-01-01), pages 55 - 64, XP002322464, ISSN: 0022-1759
BOEUF PHILIPPE ET AL: "CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use.", BMC IMMUNOLOGY [ELECTRONIC RESOURCE]. 2005, vol. 6, no. 1, 2005, pages 5, XP009067336, ISSN: 1471-2172
BOEUF PHILIPPE ET AL: "CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use.", BMC IMMUNOLOGY [ELECTRONIC RESOURCE]. 2005, vol. 6, no. 1, 2005, pages 5, XP009067336, ISSN: 1471-2172
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