QUANTITATIVE DETECTION METHOD FOR TARGET BASED ON AIR PRESSURE DETECTION

Title:

QUANTITATIVE DETECTION METHOD FOR TARGET BASED ON AIR PRESSURE DETECTION

Document Type and Number:

WIPO Patent Application WO/2015/188512

Kind Code:

A1

Abstract:

Disclosed is a quantitative analysis method based on air pressure detection, which can be used for the high-sensitivity quantitative detection of various targets having inorganic ions, micromolecules and biological macromolecules such as proteins, DNA, and even viruses, bacteria, cells, etc. The present invention catalyzes hydrogen peroxide to generate a large amount of gas using enzymes or nanometer particles, etc.; converts a target molecule detection signal into a gas pressure intensity signal; achieves signal amplification; and finally converts the pressure intensity change into an electrical signal to conduct a reading through a barometer, thereby achieving high-sensitivity quantitative detection. In the present invention, the feasibility, wide applicability and reliability of the present invention are certified through three different detection systems, i.e. an ELISA system, a DNA hydrogel and a functional DNA sensor, respectively, using a barometer.

Inventors:

YANG CHAOYONG (CN)
GUAN ZHICHAO (CN)
JIA SHASHA (CN)
ZHU ZHI (CN)
LIU DAN (CN)
ZHANG MINGXIA (CN)
LIN SHUICHAO (CN)
LI JIUXING (CN)
ZHUANG ZHIXIA (CN)

Application Number:

PCT/CN2014/087243

Publication Date:

December 17, 2015

Filing Date:

September 24, 2014

Export Citation:

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Assignee:

UNIV XIAMEN (CN)

International Classes:

G01N33/50; G01N7/18

Foreign References:

CN103994946A	2014-08-20
CN101446538A	2009-06-03
CN101571540A	2009-11-04
CN103913542A	2014-07-09
CN103889556A	2014-06-25
CN101553579A	2009-10-07
US4311794A	1982-01-19
US4065357A	1977-12-27

Other References: