Title:
QUANTUM DOT FLUORESCENCE ENHANCED IMMUNOASSAY
Document Type and Number:
WIPO Patent Application WO/2015/122391
Kind Code:
A1
Abstract:
Provided is a method for detecting a target substance in a sample, including: an incubation step in which a first probe and a second probe are added to the sample and to a negative contrast that does not include the target substance and incubation is performed, said first probe being bonded to metal nanoparticles fixed in a carbon nanotube and said second probe being bonded to quantum dots; a fluorescent light measurement step in which the fluorescent light intensity of the sample and the quantum dots in the negative contrast, after the incubation step, is measured; and a determination step in which a determination is made that the target substance is present in the sample if the fluorescent light intensity of the quantum dots in the sample is greater than the fluorescent light intensity of the quantum dots in the negative contrast. The first and second probes bond to the target substance but do not bond to each other. The metal nanoparticles and the quantum dots move closer as a result of the bonding of the first and second probes to the target substance, and the fluorescent light intensity of the quantum dots increases.
Inventors:
PARK ENOCH Y (JP)
LEE JAE WOOK (JP)
LEE JAE BEOM (KR)
LEE JAE WOOK (JP)
LEE JAE BEOM (KR)
Application Number:
PCT/JP2015/053572
Publication Date:
August 20, 2015
Filing Date:
February 10, 2015
Export Citation:
Assignee:
UNIV SHIZUOKA NAT UNIV CORP (JP)
PUSAN NAT UNIVERSITY (KR)
PUSAN NAT UNIVERSITY (KR)
International Classes:
G01N33/543; G01N21/64; G01N33/553
Foreign References:
| JP2014021016A | 2014-02-03 | |||
| JP2007234962A | 2007-09-13 | |||
| JP2003322653A | 2003-11-14 | |||
| US20120178640A1 | 2012-07-12 | |||
| US20090004117A1 | 2009-01-01 |
Other References:
DU, D.: "Covalent coupling of organophosphorus hydrolase loaded quantum dots to carbon nanotube/Au nanocomposite for enhanced detection of methyl parathion", BIOSENSORS AND BIOELECTRONICS, vol. 25, no. 6, 15 February 2010 (2010-02-15), pages 1370 - 1375, XP026833914
GUIFEN JIE: "Electrochemiluminescence immunosensor based on nanocomposite film of CdS quantum dots-carbon nanotubes combined with gold nanoparticles-chitosan", ELECTROCHEMISTRY COMMUNICATIONS, vol. 12, no. 1, 2010, pages 22 - 26, XP026813075
LEE, J.: "A plasmon-assisted fluoro-immunoassay using gold nanoparticle-decorated carbon nanotubes for monitoring the influenza virus", BIOSENSORS AND BIOELECTRONICS, vol. 64, 16 September 2014 (2014-09-16), pages 311 - 317, XP055219167, DOI: doi:10.1016/j.bios.2014.09.021
GUIFEN JIE: "Electrochemiluminescence immunosensor based on nanocomposite film of CdS quantum dots-carbon nanotubes combined with gold nanoparticles-chitosan", ELECTROCHEMISTRY COMMUNICATIONS, vol. 12, no. 1, 2010, pages 22 - 26, XP026813075
LEE, J.: "A plasmon-assisted fluoro-immunoassay using gold nanoparticle-decorated carbon nanotubes for monitoring the influenza virus", BIOSENSORS AND BIOELECTRONICS, vol. 64, 16 September 2014 (2014-09-16), pages 311 - 317, XP055219167, DOI: doi:10.1016/j.bios.2014.09.021
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
Yoshiki Hasegawa (JP)
Yoshiki Hasegawa (JP)
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