Title:
QUICK DETECTION METHOD FOR FOOD ALLERGENS BASED ON QUANTUM DOT FLUORESCENCE
Document Type and Number:
WIPO Patent Application WO/2020/199501
Kind Code:
A1
Abstract:
Disclosed is a quick detection method for food allergens based on quantum dot fluorescence, wherein same falls within the fields of food safety and biotechnology. The above-mentioned method comprises: step 1: using a carbodiimide method to perform covalent coupling of a rabbit anti-shrimp polyclonal antibody to the surface of a quantum dot to form a quantum dot fluorescence probe; step 2: constructing a fluorescent immunochromatographic test strip; and step 3: mixing the quantum dot fluorescence probe in step 1 with a sample to be detected, and then carrying out a chromatographic detection. In the present invention, quantum dot have the advantages of a uniform appearance, stable characteristics and a modifiable surface, and same can greatly optimize the state of a reaction system, thereby providing higher sensitivity, repeatability and stability, and can effectively lower the detection limit thereof, thus making the results more reliable.
Inventors:
LI ZHENXING (CN)
WANG YAQING (CN)
LIN HONG (CN)
WANG YAQING (CN)
LIN HONG (CN)
Application Number:
PCT/CN2019/104656
Publication Date:
October 08, 2020
Filing Date:
September 06, 2019
Export Citation:
Assignee:
OCEAN UNIV CHINA (CN)
International Classes:
G01N33/533; G01N21/64; G01N33/558; G01N33/68
Foreign References:
| CN109884293A | 2019-06-14 | |||
| CN101881769A | 2010-11-10 | |||
| CN106093322A | 2016-11-09 | |||
| CN109212208A | 2019-01-15 | |||
| CN105548556A | 2016-05-04 |
Attorney, Agent or Firm:
QINGDAO ZHIJIA INTELLECTUAL PROPERTY AGENCY FIRM (GENERAL PARTNERSHIP) (CN)
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