R1 PROTEIN FROM WHEAT AND THE USE THEREOF FOR THE PRODUCTION OF MODIFIED STRACH

Title:

R1 PROTEIN FROM WHEAT AND THE USE THEREOF FOR THE PRODUCTION OF MODIFIED STRACH

Document Type and Number:

WIPO Patent Application WO/2000/077229

Kind Code:

A2

Abstract:

Nucleic acid molecules are described encoding an R1-protein from wheat and methods and recombinant DNA molecules for the production of transgenic plant cells and plants synthesizing a modified starch. Additionally, the plant cells and plants resulting from those methods as well as the starch obtainable therefrom are described.

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Inventors:

ABEL GERNOT
LOERZ HORST
LUETTICKE STEPHANIE
SCHMIDT RALF-CHRISTIAN

Application Number:

PCT/EP2000/005064

Publication Date:

December 21, 2000

Filing Date:

June 02, 2000

Export Citation:

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Assignee:

AVENTIS CROPSCIENCE GMBH (DE)

International Classes:

A01H5/00; A01H5/10; C07K14/415; C08B30/00; C08B31/00; C12N5/10; C12N9/00; C12N15/09; C12N15/29; C12N15/54; C12N15/82; C12P21/08; C12Q1/68; (IPC1-7): C12N15/82; A01H5/00; C07K14/415; C07K16/16; C08B30/00; C12N5/10; C12N9/00; C12N15/52; C12Q1/68

Domestic Patent References:

WO1998027212A1	1998-06-25
WO1997011188A1	1997-03-27
WO1999053072A1	1999-10-21
WO2000028052A2	2000-05-18

Other References:

LORBERTH ET AL: "inhibiton of a starch-granule-bound protein leads to modified starch and repression of cold sweetening" NATURE BIOTECHNOLOGY,NATURE PUBLISHING,US, vol. 16, May 1998 (1998-05), pages 473-477, XP002111459 ISSN: 1087-0156 -& DATABASE EMBL [Online] ACCESSION NO: Y09533, 30 June 1998 (1998-06-30) LORBERTH R., ET AL.: "S.tuberosum mRNA for protein involved in starch metabolism" XP002136857
DATABASE EMBL [Online] ACCESSION NO: C71741, 19 September 1997 (1997-09-19) SASAKI, T.: "Rice cDNA, partial sequence (E0169_1A)." XP002111458
RAHMAN S ET AL: "THE MAJOR PROTEINS OF WHEAT ENDOSPERM STARCH GRANULES" AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY,AU,CSIRO, MELBOURNE, vol. 22, 1995, pages 793-803, XP002043127 ISSN: 0310-7841

Attorney, Agent or Firm:

VOSSIUS & PARTNER GBR (Patentanwälte European Patent Attorneys Siebertstrasse 4 München, DE)

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Claims:

1.

A nucleic acid molecule encoding an R1protein or part or derivative of R1 protein selected from the group consisting of: (a) a nucleic acid molecule encoding an R1 protein comprising a polypeptide selected from the group consisting of Seq. ID No. 2 and Seq. ID No. 10 or part or derivative thereof; (b) a nucleic acid molecule selected from the group consisting of Seq. ID No. 1 and Seq. ID No. 9 or part or derivative thereof; (c) a nucleic acid molecule comprising the coding region of the cDNA insert selected from the group consisting of plasmid pTa R111 according to DSM No. 12810 and plasmid RS2688 according to DSM No. 13511 or part or derivative thereof; (d) a nucleic acid molecule encoding a polypeptide comprising the polypeptide encoded by the cDNA insert selected from the group consisting of plasmid pTa R111 according to DSM No. 12810 and plasmid RS2688 according to DSM No. 13511 or part or derivative thereof.

2.	A nucleic acid molecule according to claim 1 comprising one or more regulatory elements which ensure transcription and/or translation in a cell.

3.	A nucleic acid molecule according to one or more of claims 1 to 2, which is a DNAmolecule.

4.	A nucleic acid molecule according to one or more of claims 1 to 2, which is an RNA molecule.

5.	A vector comprising a nucleic acid molecule according to one or more of claims 1 to 4.

6.	The vector according to claim 5, comprising one or more regulatory elements which ensures transcription and/or translation in a bacterial and/or a plant cell.

7.	A transgenic host cell, comprising a nucleic acid molecule according to one or more of claims 1 to 4 and/or a vector according to one or more of claims 5 to 6.

8.	The host cell according to claim 7, which is a plant cell.

9.

A process for the preparation of a transgenic cell according to one or more of claims 7 to 8 comprising the step of introducing a nucleic acid molecule according to one or more of claims 1 to 4 and/or a vector according to one or more of claims 5 to 6 into the genome of a procaryotic or eucaryotic cell.

10.	The process according to claim 9 wherein said cell is a plant cell.

11.	A transgenic plant comprising the host cell according to claim 8.

12.	A process for the production of a plant according to claim 11 comprising the steps of introducing into a plant cell a nucleic acid molecule according to one or more of claims 1 to 4 and/or a vector according to one or more of claims 5 to 6 and regenerating a whole plant from the plant cell.

13.	A propagation material from the plant according to claim 11.

14.	A seed from the transgenic plant according to claim 11.

15.	A process for the production of a starch comprising the step of introducing a plant cell according to claim 8, a plant according to claim 11, a propagation material according to claim 13 and/or a seed according to claim 14 into a process for the production of starch.

16.	A starch obtainable from a plant cell according to claim 8, a plant according to claim 11, a propagation material according to claim 13 and/or a seed according to claim 14 or by the process according to claim 15.

17.	A process for the production of a modified starch comprising the step of introducing a starch according to claim 16 into a process of chemical and/or physical modification of starch.

18.

A process for the production of an R1polypeptide according to Seq. ID No. 2 and/or Seq. ID No. 10 or derivatives or parts thereof comprising the steps of cultivating the host cell according to one or more of claims 7 to 8 under conditions allowing for the expression of the protein and isolating said R1 polypeptide from said cells and/or the culture medium.

19.	An R1polypeptide encoded by the nucleic acid molecule according to one or more of claims 1 to 4 or derivative or part thereof.

20.	The use of an R1polypeptide according to claim 19 for the production of a monoclonal or polyclonal antibody.

21.	The use of a nucleic acid molecule or derivative or part thereof according to one or more of claims 1 to 4 for the screening of nucleic acid libraries and/or as a probe for hybridisation, said nucleic acid molecule or derivative or part thereof being optionally labelle.

22.	The use of a nucleic acid molecule or derivative or part thereof according to one or more of claims 1 to 4 for the preparation of a transgenic cell or transgenic plant.

Description:

INTERNATIONAL SEARCH REPORT lnte lonal Application No PCT/EP 00/05064 C.(Continuation) DOCUMENTS CONSIDERED TO BE RELEVANT Category Citation of document, with indication, where appropriate, of the relevant passages Relevant to claim No. P, X WO 00 28052 A (PLANTTEC BIOTECHNOLOGIE 1-22 GMBH) 18 May 2000 (2000-05-18) the whole document X LORBERTH ET AL :"inhibiton of a 1-22 starch-granule-bound protein leads to modified starch and repression of cold sweetening" NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 16, May 1998 (1998-05), pages 473-477, XP002111459 ISSN: 1087-0156 the whole document -& DATABASE EMBL'Online ! ACCESSION NO : Y09533, 30 June 1998 (1998-06-30) LORBERTH R., ET AL.:"S. tuberosum mRNA for protein involved in starch metabolism" XP002136857 abstract X DATABASE EMBL'Online ! 1 ACCESSION NO : C71741, 19 September 1997 (1997-09-19) SASAKI, T.:"Rice cDNA, partial sequence (E0169_lA)." XP002111458 the whole document A RAHMAN S ET AL:"THE MAJOR PROTEINS OF 1-22 WHEAT ENDOSPERM STARCH GRANULES" AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY, AU, CSIRO, MELBOURNE, vol. 22,1995, pages 793-803, XP002043127 ISSN: 0310-7841 the whole document 1 INTERNATIONAL SEARCH REPORT | Inte ionalApplicationNo ! nte tona) App!! cat! on No Information on patent family members PCT/EP 00/05064 Patent document Publication Patent family Publication cited in search report date member (s) date WO 9827212 A 25-06-1998 DE 19653176 A 25-06-1998 AU 5857798 A 15-07-1998 EP 0950107 A 20-10-1999 WO 9711188 A 27-03-1997 DE 19534759 A 20-03-1997 DE 19547733 A 26-06-1997 AU 715944 B 10-02-2000 AU 7131396 A 09-04-1997 CA 2231774 A 27-03-1997 EP 0851934 A 08-07-1998 HU 9900510 A 28-05-1999 JP 11512286 T 26-10-1999 WO 9953072 A 21-10-1999 AU 3478499 A 01-11-1999 WO 0028052 A 18-05-2000 AU 1380800 A 29-05-2000

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