RAMAN QUANTIFICATION METHOD OF CANCER-RELATED SUBSTANCE

Title:

RAMAN QUANTIFICATION METHOD OF CANCER-RELATED SUBSTANCE

Document Type and Number:

WIPO Patent Application WO/2014/181816

Kind Code:

A1

Abstract:

[Problem] To provide a Raman quantification method for measuring cancer cell-derived free DNA. [Solution] This Raman quantification method is characterized by involving a step for preparing a biochip having a mesocrystal region of a silver oxide containing peroxide silver, adding blood serum or biological sample solution dropwise onto the mesocrystal region of said biochip, selectively adsorbing the cancer-related substance having a positive charge in the sample, irradiating the adsorbed cancer-related substance with a laser and detecting Raman scattering therefrom, wherein the cancer illness is determined on the basis of the intensity of the surface-enhanced Raman spectroscopy (SERS). In the carbon-specific D band and G band in the Raman scattering spectrum, a characteristic peak spectrum of the cancer-related substance can be detected in the proximity of the methyl group-characteristic 2900cm-1.

Inventors:

ITO HIROAKI (JP)
HASEGAWA YUKI (JP)
HASEGAWA KATSUYUKI (JP)

Application Number:

PCT/JP2014/062318

Publication Date:

November 13, 2014

Filing Date:

May 08, 2014

Export Citation:

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Assignee:

MYTECH CO LTD (JP)
ITO HIROAKI (JP)

International Classes:

G01N21/65; G01N33/543; G01N33/68

Domestic Patent References:

WO2013065747A1	2013-05-10
WO2012033097A1	2012-03-15
WO2010101209A1	2010-09-10
WO2008090930A1	2008-07-31

Foreign References:

JP2013133475A	2013-07-08
JP2007525662A	2007-09-06
JP2009014491A	2009-01-22
US6143529A	2000-11-07
US20040259101A1	2004-12-23
JP2011081001A	2011-04-21

Other References: