Title:
REACTION METHOD, REACTION APPARATUS AND ENZYME
Document Type and Number:
WIPO Patent Application WO/2001/031066
Kind Code:
A1
Abstract:
A reaction method wherein a mediator in an activated state is poured into a reaction system containing a substrate. In this method, it is preferable to activate the mediator under mild conditions with the use of an enzyme as a preliminary step. It is particularly preferable that a highly active mediator, which has been improved in the activity and/or stability, is poured into the above-described reaction system. A method of enzymatically decomposing a substrate comprising immobilizing an oxidizing enzyme peroxidase in a structural unit which has almost the same size as the enzyme size and a structural stability, and decomposing the substrate in the presence of an oxidizing agent at such a concentration as being accepted by the immobilization. A reaction promoter which is to be used in a reaction system of decomposing a substrate with an oxidizing enzyme, has a diketone structure allowing radicalization due to the function of the oxidizing enzyme and is soluble in water. A manganese peroxidase liquor and manganese peroxidase obtained by culturing a white rot fungus variant SC26 strain.
Inventors:
KAJINO TSUTOMU (JP)
TAKAHASHI HARUO (JP)
SUGIYAMA HIDEHIKO (JP)
ASAMI OSAMU (JP)
SASAKI TOSHIYA (JP)
LI BO (JP)
TAKAHASHI HARUO (JP)
SUGIYAMA HIDEHIKO (JP)
ASAMI OSAMU (JP)
SASAKI TOSHIYA (JP)
LI BO (JP)
Application Number:
PCT/JP2000/007618
Publication Date:
May 03, 2001
Filing Date:
October 30, 2000
Export Citation:
Assignee:
TOYODA CHUO KENKYUSHO KK (JP)
KAJINO TSUTOMU (JP)
TAKAHASHI HARUO (JP)
SUGIYAMA HIDEHIKO (JP)
ASAMI OSAMU (JP)
SASAKI TOSHIYA (JP)
LI BO (JP)
KAJINO TSUTOMU (JP)
TAKAHASHI HARUO (JP)
SUGIYAMA HIDEHIKO (JP)
ASAMI OSAMU (JP)
SASAKI TOSHIYA (JP)
LI BO (JP)
International Classes:
C12M1/40; C12N9/08; C12N11/14; C12S3/12; D21C5/00; D21C9/10; (IPC1-7): C12S3/12; C12M1/40; C12N9/12; C12N11/14; D21C3/00; D21C9/10
Domestic Patent References:
| WO1996018770A2 | 1996-06-20 |
Foreign References:
| JP5085677B2 | 2012-11-28 | |||
| JPH1112972A | 1999-01-19 | |||
| JPH0598481A | 1993-04-20 | |||
| JPH0598482A | 1993-04-20 | |||
| JPH1090214A | 1998-04-10 | |||
| JPH06148383A | 1994-05-27 | |||
| EP0937776A2 | 1999-08-25 | |||
| EP0406617A2 | 1991-01-09 |
Other References:
T. LALOT ET AL.: "A kinetic approach to acrylamide radical polymerization by horse radish peroxidase-mediated initiation", POLYM. INT., vol. 48, no. 4, April 1999 (1999-04-01), pages 288 - 292, XP002937049
S. KAWAI ET AL.: "Fluorometric determination of hydrogen peroxidase with catalase-hantzsch reaction system using methyl acetoacetate", BUNSEKI KAGAKU, vol. 33, no. 2, 1984, pages E73 - E76, XP002937050
GLENN J K ET AL.: "Purification and Characterization of an Extracellular Mn(II)-Dependent Peroxidase from the Lignin-Degrading Basidiomycete, Phanerochaete chrysosporium", ARCH. BIOCHEM. BIOPHYS., vol. 242, no. 2, 1985, pages 329 - 341, XP001009503
S. KAWAI ET AL.: "Fluorometric determination of hydrogen peroxidase with catalase-hantzsch reaction system using methyl acetoacetate", BUNSEKI KAGAKU, vol. 33, no. 2, 1984, pages E73 - E76, XP002937050
GLENN J K ET AL.: "Purification and Characterization of an Extracellular Mn(II)-Dependent Peroxidase from the Lignin-Degrading Basidiomycete, Phanerochaete chrysosporium", ARCH. BIOCHEM. BIOPHYS., vol. 242, no. 2, 1985, pages 329 - 341, XP001009503
Attorney, Agent or Firm:
Kitagawa, Osamu (Fujimigaoka Meito-ku Nagoya-shi, Aichi, JP)
Download PDF: