Title:
REACTION MIXTURE FOR CELL-FREE PROTEIN SYNTHESIS, CELL-FREE PROTEIN SYNTHESIS METHOD IN WHICH SAME IS USED, AND KIT FOR CELL-FREE PROTEIN SYNTHESIS
Document Type and Number:
WIPO Patent Application WO/2018/198542
Kind Code:
A1
Abstract:
The present invention pertains to a reaction mixture for cell-free protein synthesis, said reaction mixture including a cell extract obtained from Escherichia coli and glycerol. The present invention also pertains to a cell-free protein synthesis method in which said reaction mixture is used. The present invention furthermore pertains to a kit for cell-free protein synthesis, said kit including a cell extract obtained from Escherichia coli, glycerol, a template nucleic acid, and a substrate and/or energy source for synthesis of a target protein.
Inventors:
HOMMA TOSHIMASA (JP)
Application Number:
PCT/JP2018/008793
Publication Date:
November 01, 2018
Filing Date:
March 07, 2018
Export Citation:
Assignee:
SPIBER INC (JP)
International Classes:
C12P21/00; C07K14/00; C12N1/20; C12N15/09
Domestic Patent References:
| WO2004111203A1 | 2004-12-23 |
Foreign References:
| JP2005295914A | 2005-10-27 | |||
| JP2014097056A | 2014-05-29 | |||
| JP2004208640A | 2004-07-29 |
Other References:
CHEN, H. ET AL.: "Preliminary study on preparation of E. coli cell -free system for protein expression.", FRONT. CHEM. ENG. CHINA, vol. 2, no. 2, 2008, pages 224 - 229
LIAN, Q. ET AL.: "The cost-efficiency realization in the escherichia coli-based cell -free protein synthesis systems.", APPL BIOCHEM BIOTECHNOL, vol. 174, 2014, pages 2351 - 2367
LIAN, Q. ET AL.: "The cost-efficiency realization in the escherichia coli-based cell -free protein synthesis systems.", APPL BIOCHEM BIOTECHNOL, vol. 174, 2014, pages 2351 - 2367
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
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