Title:
REACTION SOLUTION FOR CELL-FREE PROTEIN SYNTHESIS, METHOD OF PREPARING THE SAME AND PROTEIN SYNTHESIS METHOD USING THE SAME
Document Type and Number:
WIPO Patent Application WO/2003/072796
Kind Code:
A1
Abstract:
It is intended to provide a reaction solution for cell-free protein synthesis which is suitable for folding a protein having a weakly reduced state, and a cell-free protein synthesis method characterized by performing a weakly reduced synthesis reaction, preferably by adding a substance catalyzing a disulfide bond exchange reaction at the early stage of a translation reaction and then further continuing the translation reaction. According to this method, a protein having a properly formed disulfide bond in its molecule and being comparable in function to the original one can be provided at a high efficiency. More specifically speaking, an antibody protein binding specifically to an antigen is provided.
Inventors:
ENDO YAETA (JP)
KAWASAKI TAKAYASU (JP)
SAWASAKI TATSUYA (JP)
KAWASAKI TAKAYASU (JP)
SAWASAKI TATSUYA (JP)
Application Number:
PCT/JP2003/002313
Publication Date:
September 04, 2003
Filing Date:
February 28, 2003
Export Citation:
Assignee:
ENDO YAETA (JP)
KAWASAKI TAKAYASU (JP)
SAWASAKI TATSUYA (JP)
KAWASAKI TAKAYASU (JP)
SAWASAKI TATSUYA (JP)
International Classes:
C07K16/12; C12P21/00; C12P21/02; (IPC1-7): C12P21/00; C07K16/12
Domestic Patent References:
| WO2000068412A1 | 2000-11-16 |
Foreign References:
| JP2003116590A | 2003-04-22 | |||
| JPH05336986A | 1993-12-21 | |||
| JPH07194A | 1995-01-06 | |||
| JPH1080295A | 1998-03-31 |
Other References:
YILLA M. ET AL.: "Early disulfide bond formation prevents heterotypic aggregation of membrane proteins in a cell-free translation system", J. CELL. BIOL., vol. 118, no. 2, 1992, pages 245 - 252, XP002969531
RYABOVA L.A. ET AL.: "Functional antibody production using cell-free translation: effects of protein disulfide isomerase and chaperones", NAT. BIOTECHNOL., vol. 15, no. 1, 1997, pages 79 - 84, XP002041753
JOHN D.C. ET AL.: "Cell-free synthesis and assembly of prolyl 4-hydroxylase: the role of the beta-subunit (PDI) in preventing misfolding and aggregation of the alpha-subunit", EMBO J., vol. 12, no. 4, 1993, pages 1587 - 1595, XP002969533
CHAUDHURI A. ET AL.: "A novel invertase from a thermophilic fungus thermomyces lanuginosus: its requirement of thiol and protein for activation", ARCH. BIOCHEM. BIOPHYS., vol. 327, no. 1, 1996, pages 98 - 106, XP002969534
See also references of EP 1489188A4
RYABOVA L.A. ET AL.: "Functional antibody production using cell-free translation: effects of protein disulfide isomerase and chaperones", NAT. BIOTECHNOL., vol. 15, no. 1, 1997, pages 79 - 84, XP002041753
JOHN D.C. ET AL.: "Cell-free synthesis and assembly of prolyl 4-hydroxylase: the role of the beta-subunit (PDI) in preventing misfolding and aggregation of the alpha-subunit", EMBO J., vol. 12, no. 4, 1993, pages 1587 - 1595, XP002969533
CHAUDHURI A. ET AL.: "A novel invertase from a thermophilic fungus thermomyces lanuginosus: its requirement of thiol and protein for activation", ARCH. BIOCHEM. BIOPHYS., vol. 327, no. 1, 1996, pages 98 - 106, XP002969534
See also references of EP 1489188A4
Attorney, Agent or Firm:
Shoji, Takashi (2-10 Iwamotocho 3-chom, Chiyoda-ku Tokyo, JP)
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